WO2013141436A1 - Récipient pour la centrifugation du sang - Google Patents

Récipient pour la centrifugation du sang Download PDF

Info

Publication number
WO2013141436A1
WO2013141436A1 PCT/KR2012/002920 KR2012002920W WO2013141436A1 WO 2013141436 A1 WO2013141436 A1 WO 2013141436A1 KR 2012002920 W KR2012002920 W KR 2012002920W WO 2013141436 A1 WO2013141436 A1 WO 2013141436A1
Authority
WO
WIPO (PCT)
Prior art keywords
blood
cap
upper case
case
lower case
Prior art date
Application number
PCT/KR2012/002920
Other languages
English (en)
Korean (ko)
Inventor
맹성호
김창용
Original Assignee
(주)세원메디텍
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주)세원메디텍 filed Critical (주)세원메디텍
Publication of WO2013141436A1 publication Critical patent/WO2013141436A1/fr

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/05—Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/14—Details; Accessories therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2221/00—Applications of separation devices
    • B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/028—Graduation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0605—Valves, specific forms thereof check valves

Definitions

  • the present invention relates to a container for blood centrifugation.
  • Plasma is a neutral, pale yellow liquid component from which blood cells are separated from blood, and contains water, platelets, growth factors, proteins, sugars, lipids, inorganic salts, and metabolites in addition to water. It keeps ions constant.
  • PRP platelet-rich plasma
  • the blood centrifuge container for separating the PRP from the blood is used a lot.
  • FIG. 1 is a cross-sectional view schematically showing a conventional blood centrifuge container.
  • the conventional blood centrifuge container 11 has a cylindrical shape, the first storing part 13 having an upper end blocked by a closing cap 15 and an opening hole 21 formed at a lower end thereof. ), A second storage part 23 which is screwed to the lower end of the first storage part 13 and has a flow path opening and closing part 25 therein, and a water level for sealing the lower end of the second storage part 23. It consists of an adjusting unit (19).
  • a rubber member 17 is provided at the center of the sealing cap 15.
  • the rubber member 17 is a rubber stopper for sealing the first storage part 13 and passes the injection needle C as shown in FIG. 2D.
  • Conventional centrifuge container 11 can not insert the needle (C) without the rubber member (17).
  • the flow path opening and closing part 25 is supported by an inner circumferential surface of the second storage part 23 and provided with a cradle 25c for providing a flow path 25b therebetween, and a blocking plug 25a fixed to the cradle 25c. It is composed.
  • the blocking plug 25a moves toward the opening hole 21 to block the opening hole 21 as the second housing part 23 moves toward the first storage part 13.
  • the water level control unit 19, the lifting cap (19a) for screwing the position adjustable to the second housing 23, and the inner side of the lifting cap (19a) of the second storage unit (23) It consists of a piston 19b inserted into the inside and a rubber member 17 located at the center of the piston 19b.
  • the piston 19b is moved up and down inside the second storage unit 23 by moving the lifting cap 19a up and down by turning the lifting cap 19a to adjust the internal volume of the second storage unit 23. do.
  • the rubber member 17 serves to pass the injection needle (C) as shown in Figure 2a.
  • FIGS. 3A to 3C are various problems of the centrifugal container 11 used as described above.
  • Figure is for explaining the problem of one of the drawings.
  • the injection needle (C) of the syringe (A) with the blood collected through the water level control unit 19 side rubber member (17) Inject into the container (11). If all the blood (B) is injected into the container 11, the needle (C) is removed, and the container 11 is mounted on a centrifuge (not shown) to proceed with centrifugation. After the centrifugation, plasma P and red blood cells R are separated up and down as shown in FIG. 2B.
  • the centrifuge separates the plasma P into platelet-poor plasma (PPP) and platelet deficient plasma (PPP) as shown in FIG. 2D. .
  • the PRP is heavier than the PPP and sinks to the lower portion of the first storage unit 13. Accordingly, in order to collect the PRP, the needle C of the syringe A is passed through the rubber member 17 of the sealing cap 15 to reach the lower portion of the first storage part 13 via the PPP layer. It should be pulled up by force.
  • the conventional blood centrifuge container 11 is configured as described above, inconvenient to use the needle (C) to inject blood or to extract the PRP, and in particular the fatal disadvantage by using the needle (C) Has That is, as illustrated in FIGS. 3A to 3C, the rubber debris 17a may be separated from the rubber member 17 during the process of puncturing the injection needle C through the rubber member 17. .
  • the rubber member 17 moves in the direction of the arrow a with respect to the tip portion of the needle C while the needle C is passed through the rubber member 17.
  • Some tissue of the member 17 may be torn and separated in the direction of the arrow b.
  • the rubber member which begins to tear as described above, is completely removed from the rubber member 17 at the moment when the tip portion of the injection needle C passes completely through the rubber member 17, thereby becoming rubber fragments 17a.
  • the rubber debris 17a may enter the inside of the container 11.
  • the size of the rubber debris (17a) is fine, it is difficult to find it is mixed in the blood or PRP, if the fine rubber debris (17a) is mixed in the finally obtained PRP, rubber debris when injecting PRP into the subcutaneous Since it is injected with PRP, serious side effects occur at the site.
  • the needle C in order for the needle C to reach the PRP layer, the needle C must pass through the PPP layer. In this way, since the needle C must pass through the PPP layer, the PPP is transferred to the PRP layer in a state of being stuck on the needle, and the purity of the PRP is reduced by that amount.
  • the tip portion of the needle must pass through the PPP layer, so that the PPP may penetrate into the needle during the extraction of the needle. Due to this problem, the needle C removed from the container 11 may be replaced with a new needle which is not used.
  • injection needle (C) since the injection needle (C) must be used as described above, there is a high risk of infection. For example, in order to separate and analyze blood of an AIDS patient, an AIDS virus is transmitted to an operator if a needle is accidentally stuck in the process of adding blood into the container 11.
  • the centrifugal container 11 having the above structure, since the first storage part 13 and the second storage part 23 are completely enclosed, and the atmospheric pressure does not reach, the infusion of blood and the inhalation of PRP are not smooth. According to the model, there is a container for centrifugation in which an air passage is formed, but the air passage serves as a passage for bacteria.
  • the present invention has been created to solve the above problems, it is possible to inject and inhale blood without using a needle, so that there is no risk of infection can be used with confidence, and can maintain the pressure in the sealed inner space at atmospheric pressure
  • the purpose of the present invention is to provide a container for blood centrifugation that allows for smooth infusion and inhalation of blood.
  • the blood centrifugal container of the present invention is centrifugally rotated by a centrifugal separator in a state in which the blood to be centrifuged is accommodated therein, thereby separating and accommodating the internal blood into parts other than plasma and plasma.
  • An upper case is closed by an airtight cap and a lower end is opened downwardly through a communication hole, and an upper case accommodating the separated plasma therein;
  • a lower case coupled to the lower end of the upper case so as to be adjustable in position with respect to the upper case and whose lower end is opened;
  • a lifting cap mounted on the lower end of the lower case to adjust a position of the lower case through position adjustment with respect to the lower case;
  • a first check valve mounted on the elevating cap and opened by an external force and passing the collected blood into the lower case;
  • An opening / closing part for opening or blocking a communication port of the upper case by following the position adjustment of the lower case with respect to the upper case;
  • the second check valve is opened by an external force, and installed in the inner space of the upper case, the upper end It characterized in that it comprises a suction tube is mounted to the second check valve and extends in the longitudinal direction downward.
  • the suction tube and the second check valve is formed in one set and a plurality are provided, any one of the suction tube of the plurality of suction tube extending relatively longer than the other suction tube, the lower end of the communication port It is characterized by reaching.
  • connection portion is coupled to the suction means for extracting the plasma from the upper case through the second check valve to the outside.
  • the second check valve Located in the lower portion of the sealing cap and opened through the through-hole to the upper cap, the valve receiving portion for communicating with the upper end of the suction tube in the lower sealing cap, the valve receiving portion is installed to be liftable inside the, And an elevating member that is pushed by the draft means to open the through hole when the draft means is coupled to the connecting portion, and elastic means for supporting the elevating member upward to allow the elevating member to block the through hole.
  • the lifting cap A through passage is formed to open the inner space of the lower case downward, wherein the first check valve;
  • An elevating member which is installed to the elevating passage, and is lifted by the blood injecting means to open the passage by fixing the blood injecting means used to inject the collected blood to the lower case side; It characterized in that it comprises an elastic means for elastically supporting the elevating member to cause the elevating member to block the passage when the external force is removed.
  • the closure cap When the blood is injected or drawn out, it is characterized in that the pressure control unit for adjusting the pressure in the container to the atmospheric pressure is further provided.
  • Ring-type filter holder is installed through the sealing cap and opened up and down, and is mounted to the inside of the filter holder, characterized in that it comprises an antibacterial filter for passing air in the thickness direction.
  • the upper portion of the sealing cap characterized in that the protective cap for blocking the contamination of the connection portion is detachably mounted.
  • the blood centrifugal container of the present invention as described above can be used safely and safely without injecting blood without using a needle, so it can be used safely without any concern about infection. It can maintain blood infusion and inhalation smoothly.
  • FIG. 1 is a cross-sectional view schematically showing a conventional blood centrifuge container.
  • FIG. 3 are diagrams for explaining the problem of the centrifugal container shown in FIG.
  • Figure 4 is a perspective view of a blood centrifuge container according to an embodiment of the present invention.
  • FIG. 5 is a cutaway exploded perspective view illustrating the sealing cap and the first and second suction tubes supported therein as shown in FIG. 4 separately.
  • 6 and 7 are diagrams for explaining the principle of plasma aspiration in the blood centrifuge container according to an embodiment of the present invention.
  • FIG. 8 is a cross-sectional view showing the overall internal structure of the blood centrifuge container according to an embodiment of the present invention.
  • FIG. 9 is a perspective view illustrating the lifting member shown in FIG. 8 separately.
  • 10 to 12 are diagrams for explaining the process of obtaining the PRP using the blood centrifuge container according to an embodiment of the present invention.
  • FIG. 4 is a perspective view of a blood centrifuge container 31 according to an embodiment of the present invention.
  • the blood centrifuge container 31 As shown, the blood centrifuge container 31 according to the present embodiment, the upper case 33 and the upper case 33 having a cylindrical shape and the sealing cap 35 is fixed to the upper end, Screwed to the lower end of the lower case 47 that can adjust the relative position with respect to the upper case 33 through the axial rotation, screwed to the lower end of the lower case 47 and three-way valve 51 Lifting cap 49 leading to the lower case 47 by passing the blood through, and the protective cap 37 is fitted to the upper portion of the sealing cap 35 to protect the connection portion 35b and the pressure regulator 39 It includes.
  • the upper case 33 and the lower case 47 is made of translucent acrylic or glass so as to check the state of the inside from the outside.
  • the upper case 33 is an upper and lower open type tube having male threads (33f and 33e in FIG. 8) formed at upper and lower ends thereof, and screwed to the sealing cap 35 and the lower case 47.
  • the sealing cap 35 is coupled to the male screw part 33f of the upper case to seal the inner space of the upper case 33.
  • the lower end of the upper case 33 is provided with a communication port (33c of FIG. 8) and the close contact portion (33g). This will be described with reference to FIG. 8.
  • the lower case 47 is a cylindrical member having a female screw portion 47e formed on an inner circumferential surface of an upper end portion thereof and a male screw portion 47c formed on a lower end portion thereof, and having the passage opening and closing portion 25 therein.
  • the flow path opening and closing part 25 serves to open and close the communication port 33c.
  • the three-way valve 51 which is a component of the blood injection means used to inject blood into the lower case 47, collects the blood passing through the blood collection needle 53 and the tube 53a, It is for supplying into the centrifugal container 31 through the lifting cap 49.
  • the three-way valve 51 has a first port 51c to which the syringe cylinder 55 is coupled, a second port 51d connected to a first check valve (49r of FIG. 8) to be described later, and the tube.
  • a body 51a having a third port (not shown) to which the 53a is coupled, and is installed at the side of the body 51a to connect the first port 51c and the third port, or a first port.
  • the switching lever 51b connects the 51c and the second port 51d.
  • Reference numeral 51e denotes a port fixing nut for fixing the second port 51d to the first check valve 49r.
  • the blood collection needle 53 is inserted into the blood vessel in the human body, and the first port 51 and the third Communicate the port. Thereafter, the syringe cylinder 55 is fixed to the first port 51, and then a piston (not shown) of the syringe is pulled out to provide a vacuum in the body 51a and the tube 53a to draw blood from the blood vessel.
  • the switching lever 51b is turned to block the third port, and the first port 51c and the second port 51d communicate with each other. Let's do it. In this state, when the blood in the syringe cylinder 55 is pushed into the first port 51c, the blood is injected into the container 31 through the second port 51d and the lifting cap 49.
  • the blood is supplied to the inside of the centrifugal container 31 without using an injection needle.
  • the blood collection needle 53 is not an injection needle that is plugged into the container 31.
  • the sealing cap 35 is provided with a connecting portion 35b and the pressure adjusting portion 39.
  • the connecting portion 35b is a cylindrical portion having a constant diameter, and the syringe cylinder 55 is inserted and fixed therein as shown in FIG. 6.
  • the type of the connection part 35b may vary depending on the type of the syringe cylinder 55.
  • each said connection part 35b is equipped with the 2nd check valve (45 of FIG. 6), respectively.
  • the second check valve 45 is normally closed and is opened when the syringe cylinder 55 is coupled to the first connecting portion 35b.
  • first suction tube 41 and the second suction tube 43 are fixed to the check valves 45, respectively.
  • the first and second suction tubes 41 and 43 extend downward as a pipe-shaped member having a constant diameter, so that the first suction tube 41 reaches the communication port 33c.
  • the second suction tube 43 has a relatively shorter length than the first suction tube 41.
  • the pressure adjusting unit 39 is for adjusting the internal pressure of the upper case 33 and the lower case 35 to atmospheric pressure, and has an antibacterial filter (39b of FIG. 6) through which air can pass.
  • the structure of the pressure regulator 39 will be described later with reference to FIG. 6.
  • the upper teburi portion of the sealing cap 35 is formed with a groove (35a).
  • the groove 35a is a portion into which the lower end of the protective cap 37 is fitted.
  • an internal thread 35n is formed on the inner circumferential surface of the sealing cap 35 to be coupled to the upper case 33.
  • FIG. 5 is a cutaway exploded perspective view separately illustrating the sealing cap 35 and the first and second suction tubes 41 and 43 supported therefrom.
  • two connecting portions 35b are formed side by side at the top of the sealing cap 35, and second check valves 45 are provided at the lower portions of the connecting portions 35b, respectively.
  • the second check valves positioned below the respective connecting portions 35b have the same structure.
  • the second check valve 45 has a cylindrical shape and is upwardly opened to the connecting portion 35b through the through hole 35g, and has a through passage 35e and a coupling portion 35m formed at the lower end thereof.
  • the elevating member 45a which can be lifted up and down in the valve accommodating part 35d, and the spring support 35f formed in the valve accommodating part 35d.
  • a spring 45b for upwardly supporting the elevating member 45a.
  • the elevating member 45a extends upwardly into the internal space of the connecting portion 35b through the through hole 35g, and a pressing portion 45e having a slit 45d formed at an upper end thereof, and the pressing portion 45e. It consists of a disk portion 45c integrally formed at the bottom of the) and having a predetermined thickness and diameter, and a spring support portion 45f which is positioned below the disk portion 45c and is inserted into the spring 45d.
  • the disk portion 45c is positioned below the through hole 35g and is elastically pressed upward by the action of the spring 45b to block the through hole 35g.
  • the outer circumferential surface of the pressing portion 45e is spaced apart from the inner circumferential surface of the through hole 35g. Therefore, when the disk portion 45c descends and opens from the through hole 35g, the sealing cap 35 is opened up and down.
  • the first suction tube 41 is a holder (41a) is fixed to the coupling portion (35m) of the lower end of the valve receiving portion (35d), the upper end of the hollow coupled to the holder (41a) It consists of a tube body 41b.
  • the tube body 41b communicates with the inside of the valve accommodation portion 35d through the holder 41a, extends in the longitudinal direction, and reaches a lower end thereof through the communication port 33c.
  • the tube body 41b of FIG. 5 is bent in the middle so that the lower end of the tube body 41b can be as close as possible to the communication port 33c.
  • the second suction tube 43 is composed of a holder 43a coupled to the lower end of the connecting portion 35b, and a tube body 43b having an upper end fixed to the holder 43a and extending vertically downward. .
  • the second suction tube 43 has the same configuration as the first suction tube 41, but the length of the tube body 43b is shorter than that of the tube body 41b of the first suction tube 41.
  • the second suction tube 43 is also a suction means used to pull out the PPP or PRP contained in the upper case 33 to the outside.
  • 6 and 7 are diagrams for explaining the principle of plasma aspiration in the blood centrifuge container according to an embodiment of the present invention.
  • the lifting member 45a accommodated in the valve accommodation portion 35d is elastically supported upward by the action of the spring 45b to block the through hole 35g.
  • the disk portion 45c is in close contact with the lower portion of the through hole 35g to block the through hole 35g.
  • the spout portion 55a is brought into close contact with the inner circumferential surface of the connecting portion 35b as its outer circumferential surface tapers and enters the inside of the connecting portion 35b.
  • the size of the spout portion 55a is designed so that the spout portion 55a can be in close contact with the inner circumferential surface of the connection portion 35b when the spout portion 55a is press-fitted into the connection portion 35b.
  • the pressure control unit 39 guides the outside air into the upper case 33 to maintain the pressure inside the upper case 33 to atmospheric pressure.
  • the pressure regulating portion 39 is in the form of a cylinder or a ring is fixed to the closure cap 35 and the filter holder (39a) to provide an internal passage, and installed inside the filter holder (39a) filter It is composed of an antibacterial filter (39b) for blocking the passage of the holder (39a), and a fixing ring (39c) for fixing the antibacterial filter (39b) to the filter holder (39a).
  • the antimicrobial filter 39b maintains a flat unfolded state by the fixing ring 39c and passes air in the thickness direction thereof. Since the antimicrobial filter 39b has an antimicrobial treatment, bacteria do not pass through.
  • FIG. 8 is a cross-sectional view showing the overall internal structure of the blood centrifuge container according to an embodiment of the present invention.
  • the male screw portion 33e of the upper case 33 and the female screw portion 47e of the lower case 47 are screwed together, whereby the lower case 47 is coupled to the upper case 33. It can be seen.
  • the upper case 33 and the lower case 47 are screwed, for example, when the lower case 47 is axially rotated with respect to the upper case 33, the lower case 47 for the upper case 33 The relative position of can be adjusted. That is, the stopper 25a provided in the lower case 47 can be moved forward and backward with respect to the communication port 33c.
  • the blocking plug 25a is made of silicon and is supported by a plurality of holders 25c extending in the radial direction.
  • a flow path 25b is formed around the blocking plug 25a.
  • a close contact portion 33g is formed at the lower end of the upper case 33.
  • the close contact portion 33g has a seal ring between the outer circumferential surface and the inner circumferential surface of the lower case 47 as a portion in close contact with the inner circumferential surface.
  • the lifting cap 49, the coupling part 49a for screwing to the lower end of the lower case 47, the coupling part 49a is integrally housed in the lower case 47
  • a piston portion 49b having a through passage 49c in the central shaft portion is provided.
  • the outer circumferential surface of the piston 49b is in close contact with the inner circumferential surface of the lower case 47 so that blood does not leak therebetween.
  • the first check valve 49r is provided in the through passage 49c so as to be openable and openable.
  • the first check valve 49r is opened when the port fixing nut 51e of the blood injection means is filled in the adapter 49m, and is automatically shut off when the port fixing nut 51e is removed.
  • the first check valve 49r is embedded in the through passage 49c and fixed to the adapter 49m having a locking protrusion 49n formed on an outer circumferential surface of the lower end thereof, and capable of lifting up and down the upper portion of the adapter 49m.
  • a lifting member 49f provided, and a spring 49d for elastically pressing the lifting member 49f toward the adapter 49m side.
  • the spring 49d elastically supports the elevating member 49f in a state where the upper end thereof is caught by a spring support jaw 49e formed on the inner circumferential surface of the through passage 49c.
  • the adapter 49m is a hollow tube that provides a passage 49p for passing blood therein, and the inner diameter of the passage 49p narrows toward the spring 49d. Further, the locking projection 49n is a protrusion that is caught by a female thread formed on the inner circumferential surface of the port fixing nut 51e.
  • the tip end of the second port 51e is inside the adapter 49m. Further, the lifting member 49f is pushed upwards, and is embedded in the upper end portion of the passage 49p and fixed. The first check valve 49r is opened.
  • FIG. 9 is a perspective view separately showing the elevating member 49f shown in FIG. 8.
  • the elevating member 49f has a constant diameter and thickness and extends downwardly integrally with the disk portion 49g for sealing the upper end of the adapter 49m and integrally with the lower portion of the disk portion 49g. But it is made of a tapered rod portion (49k) becomes thinner toward the bottom.
  • the taper rod part 49k is made thinner toward the lower part to separate the taper rod part 49k from the inner peripheral surface of the adapter 49m. In this manner, the tapered rod portion 49k should be spaced apart from the inner circumferential surface of the adapter 49m so that the flow passage of blood is secured when the disk portion 49g is pushed upward.
  • the lower end portion of the tapered rod portion 49k is straight, longer than the diameter of the second port 51d, and is supported by the upper end portion of the second port 51d without entering the second port 51d.
  • 10 to 12 are diagrams for explaining the process of obtaining a PRP using the blood centrifuge container 31 according to an embodiment of the present invention.
  • the injection plug 25a is spaced apart from the communication port 33c so that the internal spaces 33b and 47b of the upper case 33 and the lower case 47 communicate with each other.
  • the three-way valve 51 of the means is filled in the adapter 49m. As the three-way valve 51 is filled in the adapter 49m, the first check valve 49r is automatically opened.
  • the syringe cylinder 55 is fixed to the first port 51c, the blood collection needle 53 is inserted into the blood vessel, and then the syringe cylinder 55 The internal piston (not shown) is pulled out to collect blood in the syringe cylinder 55. At this time, the first port 51c and the second port 51d should be blocked.
  • the switching lever (51b in FIG. 4) is rotated to connect the first and second ports 51c and 51d to the inside of the syringe cylinder 55.
  • Blood is injected into the centrifugal separation container 31 through the second port 51d and the first check valve 49r.
  • the injected blood fills the inner space 47b of the lower case 47 and then goes up to the inner space 33b of the upper case 33 through the communication port 33c.
  • the centrifuge container 31 If the blood B is filled in the centrifuge container 31 as shown in Fig. 11, the three-way valve 51 is removed from the adapter 49m, and then the container 31 is mounted in the centrifuge. Centrifuge is performed.
  • the elevating cap 49 is rotated so that the interface between the plasma and red blood cells is communicated with the communication port 33c. ), The relative position of the lower case 47 with respect to the upper case 33 is also adjusted to block the communication port 33c with the blocking plug 25a.
  • the centrifugal container 31 in the above state is once again subjected to a centrifuge to carry out the second centrifugation.
  • the plasma inside the upper case 33 is separated into PPP and PRP. Since PRP is heavier than PPP, it accumulates at the bottom of the upper case 33.
  • the PPP of the boundary layer portion between the PPP and the PRP is clearly smaller in platelets than the PRP, but still has a certain amount of platelets, so if the amount of separated PRP is expected to be insufficient (before drawing the PRP)
  • the second suction tube 43 Through the second suction tube 43, the lower end of the PPP, that is, the PPP of the boundary layer portion with the PRP may be sucked out for some time.
  • the blood centrifugal container 31 of the present invention includes the first and second check valves, the first and second suction tubes 41 and 43, and the like. It can be sucked out.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Ecology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Vascular Medicine (AREA)
  • Anesthesiology (AREA)
  • External Artificial Organs (AREA)

Abstract

La présente invention concerne un récipient pour la centrifugation du sang. L'invention comprend : un boîtier supérieur dont l'extrémité supérieure est scellée au moyen d'un capuchon d'étanchéité et l'extrémité inférieure s'ouvre vers le bas par l'intermédiaire d'une ouverture au travers du lien ; un boîtier inférieur qui est lié au boîtier supérieur et dont l'extrémité inférieure est ouverte ; un bouchon ascendant et descendant qui est fixé de manière à permettre l'ajustement de la position dans le boîtier inférieur, et qui ajuste le volume à l'intérieur du boîtier inférieur via l'ajustement de position par rapport au boîtier inférieur ; un premier clapet anti-retour qui est monté sur le bouchon ascendant et descendant et permet au sang récolté de passer à travers de manière à être reçu dans le boîtier inférieur ; une partie d'ouverture et fermeture qui s'ouvre ou bloque l'ouverture au travers du lien du boîtier inférieur ; un second clapet anti-retour pour aspirer le plasma sanguin maintenu dans le boîtier supérieur ; et un tube d'aspiration qui est fourni dans l'espace interne du boîtier supérieur, est fixé à l'extrémité supérieure de celui-ci au second clapet anti-retour, et s'étend vers le bas dans le sens de la longueur. Le récipient pour la centrifugation du sang de la présente invention, qui est configuré comme décrit ci-dessus, permet au sang d'être injecté et aspiré même sans utiliser d'aiguille de seringue et donc l'invention peut être utilisée avec une plus grande tranquillité d'esprit puisque le risque d'infection est considérablement éliminé, et la pression de l'espace interne scellé peut être maintenue à la pression atmosphérique de telle sorte que l'injection et l'aspiration du sang se déroulent en douceur.
PCT/KR2012/002920 2012-03-19 2012-04-17 Récipient pour la centrifugation du sang WO2013141436A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020120027545A KR101162750B1 (ko) 2012-03-19 2012-03-19 혈액 원심분리용 용기
KR10-2012-0027545 2012-03-19

Publications (1)

Publication Number Publication Date
WO2013141436A1 true WO2013141436A1 (fr) 2013-09-26

Family

ID=46716298

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/002920 WO2013141436A1 (fr) 2012-03-19 2012-04-17 Récipient pour la centrifugation du sang

Country Status (2)

Country Link
KR (1) KR101162750B1 (fr)
WO (1) WO2013141436A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016022451A (ja) * 2014-07-23 2016-02-08 山川 勇 体液収容器
CN106075956A (zh) * 2016-05-27 2016-11-09 腾科宝迪（厦门）生物科技有限公司 一种富血小板血浆prp提取离心分离装置及其使用方法
CN106669875A (zh) * 2017-02-13 2017-05-17 深圳市邦沃科技有限公司 一种富血小板血浆离心管及其提取方法
CN112972783A (zh) * 2019-12-13 2021-06-18 周菁 一种恶性胸腹水中分离纯化肿瘤细胞装置
CN114423395A (zh) * 2019-09-18 2022-04-29 株式会社美迪爱 用于提取富血小板血浆的装置及利用其的提取方法
FR3120795A1 (fr) * 2021-03-19 2022-09-23 Maco Pharma Dispositif pour séparer le sang en composants sanguins
CN115350509A (zh) * 2022-09-02 2022-11-18 杭州锐健马斯汀医疗器材有限公司 分离装置
EP3912654A4 (fr) * 2019-11-11 2023-01-18 Jun Seok Lee Dispositif de séparation de composant de fluide corporel
WO2023197040A1 (fr) * 2022-04-13 2023-10-19 Skin2Neuron Pty Ltd Dispositif de traitement

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101406574B1 (ko) 2012-08-02 2014-06-27 조희민 혈액의 혈소판 농축 혈장 분리용 수집용구
KR101504819B1 (ko) * 2013-05-03 2015-03-23 동국대학교 산학협력단 원심분리용 용기
KR101557190B1 (ko) * 2013-05-31 2015-10-02 김홍 원심분리키트 및 이를 이용한 목표물질 추출방법
KR101585857B1 (ko) 2014-05-23 2016-01-15 주식회사 라온 Prp 추출키트
KR101930290B1 (ko) * 2018-01-12 2018-12-18 (주)레보메드 체액 분리 장치
KR101928399B1 (ko) * 2018-04-13 2018-12-12 이정민 줄기세포 분리키트
KR101979382B1 (ko) * 2018-11-30 2019-05-17 (주)레보메드 줄기세포를 포함하는 체액세포 분리 및 농축키트
CN113164836A (zh) * 2018-11-30 2021-07-23 H-W·海因里希 分离系统、装置及使用方法
KR102365747B1 (ko) * 2019-12-23 2022-02-22 (주)글로핀 원심분리기용 목표물질 추출키트 및 이를 이용한 목표물질 추출방법
KR20210088174A (ko) 2020-01-06 2021-07-14 주식회사 클리노믹스 혈액 성분 분리기 및 이를 이용한 혈액 성분 분리 방법
KR102425547B1 (ko) * 2020-02-24 2022-07-26 주식회사 싸이토딕스 분리 챔버, 이를 포함하는 ctc 분리 장치 및 ctc 분리 방법
KR102538325B1 (ko) * 2020-09-09 2023-06-08 주식회사 싸이토딕스 원심 분리용 챔버 및 이를 이용한 원심 분리 방법
KR102302773B1 (ko) * 2021-03-08 2021-09-15 주식회사 싸이토딕스 원심 분리용 챔버 및 이를 이용한 원심 분리 방법
KR102661299B1 (ko) * 2021-09-03 2024-04-26 티라클 주식회사 혈소판 풍부 혈장(prp)추출키트

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005524451A (ja) * 2002-05-03 2005-08-18 ハヌマン・エルエルシー 血小板を血液から単離するための方法および装置
KR20110009651A (ko) * 2010-12-31 2011-01-28 주식회사 메디사랑 Ｐｒｐ 추출을 위한 혈액분리용기, 혈액농축용기 및 이를 이용한 ｐｒｐ 추출방법
KR101026599B1 (ko) * 2010-12-30 2011-04-04 문상호 자가 혈소판 농축물질 분리용 용기
KR20110080245A (ko) * 2010-01-05 2011-07-13 방시열 일체형 분리장치
KR101110576B1 (ko) * 2009-03-16 2012-02-15 주식회사 메디사랑 피알피 및 필러 추출과 피브린 생성을 위한 혈액분리 및 농축용기

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005524451A (ja) * 2002-05-03 2005-08-18 ハヌマン・エルエルシー 血小板を血液から単離するための方法および装置
KR101110576B1 (ko) * 2009-03-16 2012-02-15 주식회사 메디사랑 피알피 및 필러 추출과 피브린 생성을 위한 혈액분리 및 농축용기
KR20110080245A (ko) * 2010-01-05 2011-07-13 방시열 일체형 분리장치
KR101026599B1 (ko) * 2010-12-30 2011-04-04 문상호 자가 혈소판 농축물질 분리용 용기
KR20110009651A (ko) * 2010-12-31 2011-01-28 주식회사 메디사랑 Ｐｒｐ 추출을 위한 혈액분리용기, 혈액농축용기 및 이를 이용한 ｐｒｐ 추출방법

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016022451A (ja) * 2014-07-23 2016-02-08 山川 勇 体液収容器
CN106075956B (zh) * 2016-05-27 2017-05-17 腾科宝迪（厦门）生物科技有限公司 一种富血小板血浆prp提取离心分离装置及其使用方法
CN106075956A (zh) * 2016-05-27 2016-11-09 腾科宝迪（厦门）生物科技有限公司 一种富血小板血浆prp提取离心分离装置及其使用方法
CN106669875B (zh) * 2017-02-13 2022-07-01 深圳市邦沃科技有限公司 一种富血小板血浆离心管及其提取方法
CN106669875A (zh) * 2017-02-13 2017-05-17 深圳市邦沃科技有限公司 一种富血小板血浆离心管及其提取方法
CN114423395A (zh) * 2019-09-18 2022-04-29 株式会社美迪爱 用于提取富血小板血浆的装置及利用其的提取方法
EP4032565A4 (fr) * 2019-09-18 2023-10-04 Medisarang Co., Ltd. Dispositif d'extraction de plasma riche en plaquettes et procédé d'extraction l'utilisant
EP3912654A4 (fr) * 2019-11-11 2023-01-18 Jun Seok Lee Dispositif de séparation de composant de fluide corporel
CN112972783A (zh) * 2019-12-13 2021-06-18 周菁 一种恶性胸腹水中分离纯化肿瘤细胞装置
CN112972783B (zh) * 2019-12-13 2024-04-05 陕西省人民医院 一种恶性胸腹水中分离纯化肿瘤细胞装置
FR3120795A1 (fr) * 2021-03-19 2022-09-23 Maco Pharma Dispositif pour séparer le sang en composants sanguins
WO2023197040A1 (fr) * 2022-04-13 2023-10-19 Skin2Neuron Pty Ltd Dispositif de traitement
CN115350509A (zh) * 2022-09-02 2022-11-18 杭州锐健马斯汀医疗器材有限公司 分离装置
CN115350509B (zh) * 2022-09-02 2023-11-24 杭州锐健马斯汀医疗器材有限公司 分离装置

Also Published As

Publication number Publication date
KR101162750B1 (ko) 2012-07-05

Similar Documents

Publication Publication Date Title
WO2013141436A1 (fr) Récipient pour la centrifugation du sang
WO2011052927A2 (fr) Bio-dispositif d'extraction de cellules souches hématopoïétiques et de cellules souches mésenchymateuses dans le sang périphérique
WO2013100700A1 (fr) Kit intégré de séparation de sang et de concentration du prp et procédé d'extraction du prp l'utilisant
WO2013094819A1 (fr) Dispositif de séparation du sang
WO2014168409A1 (fr) Récipient pour séparation du sang permettant d'extraire les plaquettes en vue d'une auto-transfusion
WO2013133529A1 (fr) Séparateur de composants
WO2019103313A2 (fr) Dispositif pour centrifugation de composants sanguins
WO2011052948A2 (fr) Unité d'extraction de cellules régénératives et système d'extraction de cellules régénératives
CN108654141B (zh) 一种富血小板血浆制备装置
KR101170146B1 (ko) 혈소판이 풍부한 성장인자 추출을 위한 혈액분리 및 농축장치
WO2013085151A1 (fr) Récipient pour séparateur centrifuge
WO2014112794A1 (fr) Dispositif destiné à extraire les cellules régénératives, système permettant d'extraire les cellules régénératives et procédé permettant d'extraire les cellules régénératives qui utilise ces derniers
WO2017119693A1 (fr) Seringue jetable pourvue de fonction de filtre de sécurité
WO2021210809A1 (fr) Connecteur multi-flacons
WO2020085688A1 (fr) Dispositif de séparation de cellules souches adipeuses
KR101406574B1 (ko) 혈액의 혈소판 농축 혈장 분리용 수집용구
WO2017164528A1 (fr) Seringue à filtre
CN210935419U (zh) 一种富血小板血浆离心装置
WO2021054667A1 (fr) Dispositif d'extraction de plasma riche en plaquettes et procédé d'extraction l'utilisant
WO2022211293A1 (fr) Bio-kit pour extraire un fluide corporel contenant des cellules souches
CN110856775A (zh) 医用分离提取装置和借此分离和提取富血小板血浆的方法
WO2016195126A1 (fr) Appareil d'extraction et de séparation du sang pour extraire des plaquettes riches en facteur de croissance
CN206534913U (zh) 一种血液采集管
WO2015037845A1 (fr) Séparateur de constituants
WO2013055070A2 (fr) Trousse pour l'extraction de plasma

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12872196

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205 DATED 25/11/2014)

122 Ep: pct application non-entry in european phase

Ref document number: 12872196

Country of ref document: EP

Kind code of ref document: A1