WO2013094819A1 - Dispositif de séparation du sang - Google Patents

Dispositif de séparation du sang Download PDF

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Publication number
WO2013094819A1
WO2013094819A1 PCT/KR2012/001235 KR2012001235W WO2013094819A1 WO 2013094819 A1 WO2013094819 A1 WO 2013094819A1 KR 2012001235 W KR2012001235 W KR 2012001235W WO 2013094819 A1 WO2013094819 A1 WO 2013094819A1
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WO
WIPO (PCT)
Prior art keywords
kit
blood
space
cap
opening
Prior art date
Application number
PCT/KR2012/001235
Other languages
English (en)
Korean (ko)
Inventor
황석현
신봉근
Original Assignee
(주) 레보메드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주) 레보메드 filed Critical (주) 레보메드
Priority to CN201280063492.8A priority Critical patent/CN104010672B/zh
Publication of WO2013094819A1 publication Critical patent/WO2013094819A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B01L3/5635—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors connecting two containers face to face, e.g. comprising a filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/10—Apparatus for enzymology or microbiology rotatably mounted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/24—Apparatus for enzymology or microbiology tube or bottle type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components

Definitions

  • the present invention relates to a blood separation device, and more particularly to a blood separation device for extracting PRP by centrifugation.
  • Blood is composed of plasma, platelets, and blood cells, among which platelets contain the components necessary for the regeneration and activation of human tissue.
  • the platelets thus separated are plasma-rich plasma, namely, platelet rich plasma (PRP) and platelets that are not rich in plasma, That is, it consists of Platelet Poor Plasma (PPP).
  • PRP platelet rich plasma
  • PPP Platelet Poor Plasma
  • Platelets contain several growth factors and play an important role in wound healing and skin regeneration. It is reported that such platelets are contained in high concentrations in PRP, and various growth factors stimulate the proliferation of surrounding cells and stimulate abundant synthesis of collagen and other components. Accordingly, PRP has been used in various fields such as pain treatment such as low back pain, hair loss treatment, skin diseases such as skin regeneration or burn treatment.
  • the collected blood is centrifuged to separate blood cell components and platelet poor plasma (PPP, Platelet Poor Plasma) and centrifuged again to extract only PRP.
  • PPP platelet poor plasma
  • the conventional separation method using the blood separation device has a problem that the blood may be exposed to the atmosphere during the transfer to the centrifuge vessel, the transfer to the separation vessel including the filter after the blood collection.
  • the present invention has been made to solve the above-mentioned problems of the prior art, and an object of the present invention is to provide a blood separation device capable of two centrifugal separations without exposing the blood to the atmosphere. In addition, an object of the present invention is to provide a blood separation device that can be easily separated by concentrating more PRP. It is also an object of the present invention to provide a blood separation device capable of extracting exactly the desired amount with one kit.
  • one embodiment of the present invention includes a first space for receiving blood therein, the first space is a first kit, which is separated vertically through the first opening, the blood inside And a second space accommodating the second space, wherein the second space is disposed between the second kit, the first kit, and the second kit, which are vertically separated through the second opening, and the first space and the first kit.
  • a stopper having a second bar, a first cap covering an upper end of the first kit, and a second cap covering a lower end of the second kit, wherein the stopper includes the first opening and the second opening.
  • a blood separation device characterized in that a plurality of blood separations are possible by opening and closing each part.
  • the first space and the second space may be maintained in a vacuum state prior to the injection of blood.
  • the stopper is rotatably screwed with the first kit and the second kit can be moved up and down.
  • the first kit or the second kit may be provided with an opening for injecting or extracting blood.
  • the opening may be provided with a filter for preventing contamination.
  • the first cap is rotatably screwed with the first kit can be moved up and down.
  • the second cap is screwed rotatably with the second kit can be moved up and down.
  • the first cap or the second cap may comprise a member made of silicone or rubber.
  • At least one of the first kit, the second kit, the stopper, the first cap, and the second cap may be made of a transparent material.
  • a scale line may be displayed on the first cap or the second cap.
  • the first kit or the second kit may be in the form of an hourglass separated into an upper space and a lower space.
  • a sealing ring of silicon or rubber material may be disposed between the stopper and the first kit and between the stopper and the second kit.
  • the present invention it is possible to provide a blood separation device capable of two centrifugal separations without exposing the blood to the atmosphere.
  • a blood separation device that can be easily separated by concentrating more PRP.
  • the present invention can provide a blood separation device that can accurately extract the desired amount with one kit.
  • FIG. 1 is a perspective view of a blood separation apparatus according to an embodiment of the present invention.
  • FIG. 2 is an exploded perspective view of the blood separation device of FIG. 1.
  • FIG. 2 is an exploded perspective view of the blood separation device of FIG. 1.
  • FIG. 3 and 4 are cross-sectional views of the blood separation device of FIG.
  • FIG. 5 is a partial cross-sectional view schematically showing the operation of the stopper of the blood separation device of FIG.
  • FIG. 6 is a partial perspective view showing the installation form of the filter for preventing contamination.
  • FIG 7 and 8 are photographs showing the actual appearance of the blood separation apparatus according to an embodiment of the present invention.
  • FIG. 1 is a perspective view of a blood separation device according to an embodiment of the present invention
  • Figure 2 is an exploded perspective view of the blood separation device of Figure 1
  • Figure 3 and Figure 4 is a cross-sectional view of the blood separation device of Figure 1
  • Figure 5 is Figure 1
  • Partial cross-sectional view schematically showing the operation of the stopper of the blood separation device of Figure 6 is a partial perspective view showing the installation form of the filter for preventing contamination.
  • the blood separation device 1 may include a first cap 10, a first kit 20, a stopper 30, a second kit 40, and a second cap 50. Include. These components form a total of three chambers (spaces) used for the separation of blood, which are open or closed with each other.
  • the first cap 10 is provided to cover the upper end of the first kit 20.
  • the first cap 10 is the outer side 11 in contact with the outer diameter of the upper cylindrical portion 21 of the first kit 20 to be described later, and the outer side 11 is fixedly coupled to the upper cylinder of the first kit 20
  • An inner portion 12 in contact with the inner diameter of the portion 21 is included.
  • a screw portion 13 is provided at an inner diameter of the outer portion 11 of the first cap 10, and the screw portion 13 is provided with a screw portion 26 provided at an upper cylindrical portion 21 of the first kit 20, which will be described later. Screwed rotatably. By rotating the first cap 10 with respect to the first kit 20, the first cap 10 is movable up and down with respect to the first kit 20. Thereby, the amount of blood accommodated in the inner space (first space) of the first kit 20 can be adjusted.
  • the inner side 12 of the first cap 10 is fixedly coupled to the outer side 11.
  • the protrusion provided on the side inner circumferential surface of the outer portion 11 is fitted to the groove provided on the side outer circumferential surface of the inner portion 13, so that the inner portion 12 and the outer portion 11 may be fixedly coupled. .
  • the inner portion 12 may be a member having a flexible material, such as silicone or rubber.
  • a flexible material such as silicone or rubber.
  • the first kit 20 is disposed below the first cap 10 and includes a first space for receiving blood therein.
  • the first kit 20 has an inner side between the upper cylindrical portion 21, the lower cylindrical portion 22, the upper cylindrical portion 21, and the lower cylindrical portion 22 integrally connected to the upper cylindrical portion 21.
  • Inner conical portion 23 is formed to protrude into, and includes a neck portion 24 formed on the upper side of the inner conical portion (23).
  • the neck part 24 is provided with the 1st opening part 24a, and a 1st space is divided up and down into the upper space 20a and the lower space 20b by this 1st opening part 24a.
  • An upper threaded portion 26 coupled to the threaded portion 13 of the first cap 10 is provided at an upper end of the outer surface of the upper cylindrical portion 21.
  • the lower thread part 27 which engages with the upper thread part 35 of the stopper 30 mentioned later is provided in the lower end part of the outer side surface of the lower cylindrical part 22. Screw coupling between the lower cylindrical portion 22 and the stopper 30 will be described later.
  • the inner conical part 23 is formed in the shape of a cone whose diameter decreases upward toward the inner space (first space) at the portion where the upper cylindrical portion 21 and the lower cylindrical portion 22 contact each other.
  • the upper end of the inner cone 23 is provided with a tubular neck 24 formed to extend in the upward direction.
  • the first space is separated up and down through the neck 24, and the components of the blood are freely moved up and down during centrifugation of the blood.
  • the neck 24 is closed to spatially separate the blood.
  • the side portion of the inner cone 23 is provided with an opening 25 for injecting or extracting blood.
  • a cap (not shown) can be provided in close contact with the opening 25.
  • the cap may be made of silicone or rubber material, thereby allowing the blood in the syringe to be injected into the blood separation apparatus 1 or the blood having been separated can be extracted outside through the syringe.
  • the opening 25 may be provided with a filter for preventing contamination.
  • the pollution prevention filter 251 is installed in close contact with the opening 25, and the sealing ring 252 is fixedly coupled to the filter 251.
  • an opening cap 253 is provided on the sealing ring 252.
  • the stopper 30 includes an outer cylindrical portion 31, a plate 32 located at the inner diameter center portion of the outer cylindrical portion 31, a first bar 33 protruding upward from an upper surface of the central portion of the plate 32, And a second bar 34 protruding downward from the lower surface of the central portion of the plate 32.
  • the upper threaded portion 35 is engaged with the lower threaded portion 27 of the first kit 20 described above.
  • the first kit 20 can be moved up and down with respect to the stopper 30, thereby allowing the first kit 20 to be moved.
  • the first opening portion 24a can be opened and closed.
  • the second kit 40 can be moved up and down with respect to the stopper 30, thereby allowing the second kit 40 to be moved.
  • the second opening 43a can be opened and closed.
  • the first opening 24a of the first kit 20 is opened by moving the first kit 20 upward with respect to the stopper 30, and the second opening with respect to the stopper 30.
  • the kit 40 downward By moving the kit 40 downward, the state in which the second opening 43a of the second kit 40 is opened can be confirmed.
  • the sealing ring 60 may be disposed between the stopper 30 and the first kit 20.
  • the sealing ring 60 is a member made of silicone or rubber, thereby sealing the fluid so that fluid does not leak between the stopper 30 and the first kit 20.
  • sealing ring 70 may be disposed between the stopper 30 and the second kit 40.
  • the sealing ring 70 is a member made of silicon or rubber, thereby sealing the fluid so that fluid does not leak between the stopper 30 and the second kit 40.
  • the plate 32 is in the form of a thin plate, and the plate 32 is provided with a communication hole 32a penetrated up and down.
  • the communication hole 32a may have a spherical shape, a fan shape, or a half moon shape when viewed from the top, but is not limited to this shape.
  • the inner space (first space) of the first kit 20 described above and the inner space (second space) of the second kit 40 described later are in fluid communication with the communication hole 32a. That is, the lower space 20b of the first kit 20 functions substantially integrally with the upper space 40a of the second kit 40 to be described later.
  • the first bar 33 and the second bar 34 are members of a long cylindrical shape having a constant diameter, but are not limited to such a shape. End portions of the first bar 33 and the second bar 34 are in contact with the first opening 24a and the second opening 43a to prevent blood flow, and the first bar 33 and the second bar 34 may prevent the flow of blood. End portions of the bar 33 and the second bar 34 may be formed in a hemispherical shape.
  • the second kit 40 is disposed below the stopper 30 and includes a second space for receiving blood therein.
  • the second kit 20 is integrally connected to the upper cylindrical portion 41, the upper cylindrical portion 41, and the upper connecting portion 42, the diameter of which becomes smaller toward the lower side, integral with the lower end of the upper connecting portion 42.
  • Tube-shaped neck portion 43 connected to the lower, the lower connection portion 44, which is integrally connected to the neck portion 43 and the diameter increases gradually toward the lower side, the lower cylindrical portion 45 integrally connected to the lower connection portion 44 ).
  • a second opening 43a is provided in the neck 43, and the second opening 43a separates the second space into the upper space 40a and the lower space 40b up and down. That is, the second kit 40 has an hourglass shape that is divided into an upper space 40a and a lower space 40b.
  • An upper threaded portion 47 coupled to the threaded portion 36 of the stopper 30 is provided at the upper end of the outer surface of the upper cylindrical portion 41.
  • a lower threaded portion 48 is provided at the lower end of the outer surface of the lower cylindrical portion 45 to engage with the threaded portion 52 of the second cap 50 described later. Screw coupling between the lower cylindrical portion 45 and the second cap 50 will be described later.
  • the neck 43 of the second kit 40 While the second space is separated up and down through the neck 43 of the second kit 40, the components of the blood are freely moved up and down during centrifugal separation of the blood. In addition, when the centrifugation of blood is completed, the neck 43 is closed to spatially separate the blood.
  • the side portion of the upper connection portion 42 is provided with an opening 46 for injecting or extracting blood.
  • a cap (not shown) can be provided in close contact with the opening 46.
  • the cap may be made of silicone or rubber material, thereby allowing the blood in the syringe to be injected into the blood separation apparatus 1 or the blood having been separated can be extracted outside through the syringe.
  • the second cap 50 is positioned below the second kit 40 and is provided to cover the lower end of the second kit 40.
  • the second cap 50 includes an outer portion 51 in contact with the outer diameter of the lower cylindrical portion 45 of the second kit 40 described above.
  • a screw portion 52 is provided at an inner diameter of the outer portion 51 of the second cap 50, and the screw portion 52 is provided with a screw portion 48 provided in the lower cylindrical portion 45 of the second kit 40 described above. Screwed rotatably.
  • the second cap 50 By rotating the second cap 50 with respect to the second kit 40, the second cap 50 is movable up and down with respect to the second kit 40.
  • the amount of blood accommodated in the inner space of the second kit 40 can be adjusted. For example, in FIG. 3, since the second cap 50 is moved downward with respect to the second kit 40, the volume of the lower space 40b is increased, and in FIG. 4, the second cap 50 is second. Since the state moves upward with respect to the kit 40, the volume of the lower space 40b becomes small.
  • the sealing ring 80 may be disposed between the second cap 50 and the second kit 40.
  • the sealing ring 80 is a member made of silicone or rubber, thereby sealing the fluid so that fluid does not leak between the second cap 50 and the second kit 40.
  • the blood separation device 1 includes three space parts, that is, the upper space 20a of the first kit 20, the lower space 40b of the second kit 40, and the first kit which is an integral space. It is divided into the lower space 20b of 20 and the upper space 40a of the second kit 40.
  • the upper space 20a and the lower space 20b of the first kit 20 are separated by the first opening 24a
  • the upper space 40a and the lower space 40b of the second kit 40 are separated. Is separated by the second opening 43a.
  • the stopper 30 opens and closes the first opening portion 24a and the second opening portion 43a, respectively, so that a plurality of blood separations can be performed. This will be described later.
  • the first space inside the first kit 20 and the second space inside the second kit 40 may be maintained in a vacuum state before the blood is injected.
  • the phenomenon that injection becomes difficult due to internal air pressure when supplying blood in the blood separation device 1 is prevented.
  • the vacuum in the blood separation apparatus 1 may be gradually destroyed.
  • each component of the blood separation device 1 that is, the first cap 10, the first kit 20, the stopper 30, the second kit 40, and the second cap 50.
  • Silver may be formed of a transparent material. As a result, the user can easily observe the amount or separation of blood.
  • grid lines may be displayed on the first cap 10 or the second cap 50, whereby the upper space 20a of the first kit 20 and the lower space 40b of the second kit 40 may be displayed. It is possible to accurately measure or adjust the volume of.
  • the openings 25 and 46 for injecting and extracting blood are provided in both the first kit 20 and the second kit 40, but any one of these openings 25 and 46 is provided. Only one may be provided. In addition, two or more openings may be provided in only one of the first kit 20 or the second kit 40.
  • the shapes of the first kit 20 and the second kit 40 are different from each other, but the shapes of the first kit 20 and the second kit 40 may be the same.
  • the shapes may be opposite to each other.
  • the first kit 20 may be formed in an hourglass shape.
  • the shapes of the first cap 10 and the second cap 50 are different from each other, but the shapes of the first cap 10 and the second cap 50 may be the same.
  • the shapes may be opposite to each other.
  • a member made of silicon or rubber may be provided in the second cap 50.
  • the upper threaded portion 35 and the lower threaded portion 36 are respectively provided on the inner surface of the outer cylindrical portion 31, but the upper threaded portion 35 and the lower side of the outer cylindrical portion 32 are described. It is also possible for either or both of the threads 36 to be provided on the outer side of the outer cylindrical portion 32.
  • the position of the lower thread part 27 of the 1st kit 20 and the upper thread part 47 of the 2nd kit 40 is also the upper thread part 35 and the lower thread part 36 of the outer cylindrical part 31, and It should be changed to be a joinable position.
  • FIG. 7 and 8 are photographs showing the actual appearance of the blood separation device 1 according to an embodiment of the present invention.
  • the centrifugal separation of blood is made through FIG. 7, and the blood is injected into the blood separation device 1 through FIG. 8.
  • the collected blood is injected into the blood separation device 1 through the opening 25 of the first kit 20 or the opening 46 of the second kit 40.
  • the first opening 24a of the first kit 20 is closed by the first bar 33 of the stopper 30, and the second opening 43a of the second kit 40 is open.
  • the centrifugation is performed first at.
  • the plasma and buffy coat in the blood are located in the upper space 40a of the second kit 40, and the blood cells are located in the lower space 40b.
  • the second cap 50 is rotated to appropriately adjust the volume of the lower space 40b, thereby accurately separating blood components.
  • the second opening 43a of the second kit 40 is closed by the second bar 34 of the stopper 30.
  • the blood separation device 1 is turned over, that is, the first kit 20 is positioned below the second kit 40, the first opening 24a of the first kit 20 is opened. Secondary centrifugation is performed in an open state. As a result, multiplatelet plasma (PRP) and anemia platelet plasma (PPP) are formed by dividing the layer.
  • PRP multiplatelet plasma
  • PPP anemia platelet plasma
  • the stopper 30 is rotated with respect to the first kit 20. 1 Close the first opening 24a of the kit 20. In this way, the PRP in the separated blood is extracted using a syringe through the inner portion 12 of the first cap 10.
  • the blood can be centrifuged twice in the blood separation apparatus 10 without being exposed to the outside, highly concentrated PRP can be extracted without fear of infection due to the air exposure of the blood.

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Abstract

Selon l'un de ses modes de réalisation, la présente invention concerne un dispositif de séparation du sang comprenant : un premier ensemble qui comprend un premier espace pour recevoir du sang à l'intérieur de celui-ci, le premier espace étant séparé entre une partie supérieure et une partie inférieure au moyen d'une première partie d'ouverture ; un second ensemble qui comprend un second espace pour recevoir du sang à l'intérieur de celui-ci, le second espace étant séparé entre une partie supérieure et une partie inférieure au moyen d'une seconde partie d'ouverture ; une plaque qui est disposée entre le premier ensemble et le second ensemble et qui comporte un trou de raccordement traversant pour établir une liaison traversante de fluide du premier espace et du second espace ; un bouchon ayant une première barre qui est disposée de façon à se projeter vers le haut à partir de la plaque et ouvre et ferme la première partie d'ouverture, et une seconde barre qui est disposée de façon à se projeter vers le bas à partir de la plaque et ouvre et ferme la seconde partie d'ouverture ; un premier capuchon qui couvre la partie d'extrémité supérieure du premier ensemble ; et un second capuchon qui couvre la partie d'extrémité inférieure du second ensemble. Le bouchon permet au sang d'être séparé plusieurs fois par ouverture et fermeture respectives de la première partie d'ouverture et de la seconde partie d'ouverture respectivement.
PCT/KR2012/001235 2011-12-20 2012-02-20 Dispositif de séparation du sang WO2013094819A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201280063492.8A CN104010672B (zh) 2011-12-20 2012-02-20 血液分离装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0138589 2011-12-20
KR1020110138589A KR101170028B1 (ko) 2011-12-20 2011-12-20 혈액 분리 장치

Publications (1)

Publication Number Publication Date
WO2013094819A1 true WO2013094819A1 (fr) 2013-06-27

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ID=46886831

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Application Number Title Priority Date Filing Date
PCT/KR2012/001235 WO2013094819A1 (fr) 2011-12-20 2012-02-20 Dispositif de séparation du sang

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KR (1) KR101170028B1 (fr)
CN (1) CN104010672B (fr)
WO (1) WO2013094819A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
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