WO2013066014A1 - Récipient pour séparation centrifuge permettant une séparation centrifuge rapide - Google Patents

Récipient pour séparation centrifuge permettant une séparation centrifuge rapide Download PDF

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Publication number
WO2013066014A1
WO2013066014A1 PCT/KR2012/008959 KR2012008959W WO2013066014A1 WO 2013066014 A1 WO2013066014 A1 WO 2013066014A1 KR 2012008959 W KR2012008959 W KR 2012008959W WO 2013066014 A1 WO2013066014 A1 WO 2013066014A1
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WO
WIPO (PCT)
Prior art keywords
chamber
valve body
centrifugation
centrifugal
guide
Prior art date
Application number
PCT/KR2012/008959
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English (en)
Korean (ko)
Inventor
박현정
Original Assignee
Park Hyun Jeong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Park Hyun Jeong filed Critical Park Hyun Jeong
Priority to SG11201403715SA priority Critical patent/SG11201403715SA/en
Priority to CN201280060174.6A priority patent/CN103974777A/zh
Publication of WO2013066014A1 publication Critical patent/WO2013066014A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B1/00—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Definitions

  • the present invention relates to a centrifuge container mounted on a centrifuge, and more particularly, to depositing a centrifuged material by centrifugation by applying a centrifugal force to a physiological complex fluid such as blood or bone marrow by a difference in specific gravity for each component.
  • the valve element that separates the boundary layer between the suspended solids and the suspended solids can be reciprocated in the centrifugal process, but the rotation is prevented, and the component layer desired to be obtained can be quickly obtained by securing a wide passage through which the suspended solids are separated from the sediment.
  • the present invention relates to a centrifugal container for separating and recovering.
  • centrifuge is a device that separates materials by using centrifugal force generated when an object rotates.
  • centrifuges are used for the purpose of separating by weight the material mixed with the liquid or cells having a higher specific gravity and adhesion than the liquid. Centrifugation of a complex fluid, such as blood, using a centrifuge separates the fluid into several layers due to differences in specific gravity.
  • Blood is used as a major indicator for determining various diseases and health conditions
  • platelets containing abundant growth factors in the blood are used for therapeutic purposes.
  • Blood is composed of red blood cells, white blood cells, platelets, etc.
  • platelets are mainly present in plasma, and plasma is classified into a platelet rich plasma (PRP) layer and a platelet plasma layer (PPP) layer.
  • PRP platelet rich plasma
  • PPP platelet plasma layer
  • concentrated platelets (PRP) have been used for therapeutic purposes because they play a role in stimulating stem cells around the transplanted part, especially when it is implanted in the pain area, especially in the knee seat, ligaments and muscles.
  • a centrifuge is used for this purpose.
  • Centrifuges are divided into various types according to the amount of sample to be centrifuged, the rotational speed, and the type of the rotor.
  • the centrifuge accommodates the centrifuge material and separates them into layers by the difference in specific gravity due to centrifugal force. And a plurality of chambers for decanting and recovering a portion of the separated layer.
  • Patent No. 10-0435264 discloses a plurality of chambers for receiving a material to be centrifuged, means for rotating the chambers for centrifuging the material, and a first chamber of the chambers. Means for securing the chambers to a predetermined position to discharge the suspended solids into the second chamber, wherein a disk floating in the boundary layer between the centrifuged materials is provided inside the first chamber, One side has a structure in which a groove is formed to allow the suspended solids to pass to the other side of the disc.
  • the sample vessel can be fixed in the selected direction during centrifugation or after centrifugation to separate and discharge the suspended solids between two or more chambers of the vessel. It has a structure formed with a groove for fluid communication on one side of the centrifugal process, in response to the change of the boundary layer of the material to be centrifuged in the centrifugation process, the disk is moved along the longitudinal direction of the first chamber and rotated and oscillated As the separated suspended solids do not pass smoothly by interference with the grooves formed in the disk, there is a problem in that the centrifugation takes a long time.
  • the first chamber and the second chamber are disposed side by side adjacent to each other, and the fluid communication between the chambers is made only through a bridge formed in the lid, so that the cross-sectional area of the passage where the centrifuged suspended material moves to another chamber The narrow is formed there is a problem that takes a lot of time to decanter floating material.
  • the present invention has been made to solve the above problems, the component material constituting the physiological complex fluid such as blood or bone marrow in the separation by using the difference in centrifugal force and specific gravity of each component by the rotation of the centrifuge It is an object of the present invention to provide a container for centrifugation which allows the separation of substances to be made quickly and accurately.
  • Another object of the present invention is to prevent suspended solids from mixing with suspended solids in the process of rapidly decanting the centrifuged suspended solids into another chamber, thereby maintaining the purity of the suspended solids in the decanting process, while maintaining the suspended solids between the chambers. It is to provide a centrifugal container that can easily and quickly perform the decanting operation by securing a wide cross-sectional area.
  • a centrifugal container capable of rapid centrifugation according to the present invention for achieving the above object includes a first chamber 110 in which a material for centrifugation is received and a centrifugation from a material in the first chamber 110.
  • the inside of the first chamber 110 is disposed to be linearly reciprocated along the longitudinal direction of the first chamber 110 so as to be located at the boundary layer between the centrifuged materials, and a plurality of through holes 141 on the bottom surface.
  • the floating material is centrifuged in the centrifugal process is moved through the plurality of through holes 141 and at the same time the outer surface of the valve body 140 and the first Characterized by moving through the gap formed between the inner surface of the chamber (110).
  • the inner surface of the first chamber 110 and the outer surface of the valve body 140 are based on the central axis of the first chamber 110 along a direction parallel to the longitudinal direction of the first chamber 110.
  • a plurality of guide ribs 114 and guide grooves 142 are formed at positions corresponding to each other at predetermined angular intervals, so that the reciprocating movement of the valve body 140 is allowed in the centrifugal separation process, but rotational movement is prevented. Can be configured.
  • valve body 140 is formed in a cup shape in which the top is open and the bottom surface and the side is connected, the through hole 141 formed in the bottom surface of the valve body 140 is the bottom surface of the valve body 140 It may be configured to be formed at regular intervals along the circumferential direction in the form of a central portion and concentric circles.
  • the clearance between the valve body 140 and the first chamber 110, and the through-hole 141 is allowed to pass through the floating material separated from the centrifugation target material during centrifugal separation, from the centrifugation target material
  • the sediment to be separated is preferably formed to a size that does not pass.
  • the main body 100, the second chamber (circumferentially in communication with the passage (P) to surround the outer surface of the first chamber 110 to guide the decanted floating material toward the second chamber 120 side 120 may be provided with a guide portion 115 formed to be inclined toward the side.
  • connection portion 112 for forming the passage P is provided at an upper end portion of the outer wall 111 of the first chamber 110, and the cover 200 is spaced apart from an upper circumference of the connection portion 112.
  • the protrusions 210 and the recesses 220 are formed outside the protrusions 210, and the passages P are connected to the connection part 112, the protrusions 210, and the recesses 220. It may be configured to be formed around the upper portion of the first chamber 110 by the spaced space between the stepped 211 formed in the boundary portion.
  • the bottom surface of the guide portion 115 is spaced apart from the recessed portion 220 of the cover 200 to one side of the first chamber 110 across the side where the second chamber 120 is located.
  • the upper surface 115a may be positioned, and the inclined surface 115b along the circumference of the first chamber 110 may extend from the upper surface 115a to both sides thereof, and may be connected to the second chamber 120.
  • the inclined surface 115b may have a helical shape and may be configured to have a symmetrical shape around the first chamber 110 with respect to the upper surface 115a.
  • the second chamber 120 may be configured in a shape in which the cross-sectional area is gradually narrowed from the upper end to the lower end.
  • a plurality of through-holes are formed in the bottom surface of the valve body positioned in the boundary layer between the centrifuged materials, and the outside of the valve body allows only the linear reciprocating motion while the valve body is prevented from rotating in the first chamber.
  • the size of the through-hole formed in the bottom surface of the valve body and the gap between the outer surface of the valve body and the inner surface of the first chamber to form a size that passes through the floating material, but the precipitate does not pass, thereby the centrifuged floating material In the process of decanting to the second chamber side, there is an advantage in that the precipitate material is prevented from being mixed with the suspended material to maintain the purity of the suspended material to be obtained.
  • FIG. 1 is a perspective view of a centrifugal container capable of rapid centrifugation according to an embodiment of the present invention
  • FIG. 2 is a cross-sectional view taken along line A-A of FIG.
  • FIG. 3 is an exploded perspective view of a container for centrifugation according to an embodiment of the present invention.
  • FIG. 4 is a front view of a container for centrifugation according to an embodiment of the present invention.
  • FIG. 5 is a side view of a container for centrifugation according to an embodiment of the present invention.
  • FIG. 6 is a rear view of the container for centrifugation according to an embodiment of the present invention.
  • FIG. 7 is a plan view of the main body of the container for centrifugation according to an embodiment of the present invention.
  • FIG. 8 is an exploded perspective view of a main body and a valve body of a centrifugal container according to another embodiment of the present invention.
  • 9 to 12 is a state diagram showing the centrifugation and decanting process of blood using the centrifuge container according to the present invention.
  • centrifuge container 100 main body
  • first chamber 111 outer wall
  • connecting portion 114,142a guide rib
  • valve body 141 opening
  • step 212 inlet port
  • red blood cells 302 plasma
  • FIG. 1 is a perspective view of a centrifugal container capable of rapid centrifugation according to an embodiment of the present invention
  • FIG. 2 is an AA cross-sectional view of FIG. 1
  • FIG. 3 is an exploded perspective view of a centrifugal container according to an embodiment of the present invention.
  • 4 is a front view of a centrifugal container according to an embodiment of the present invention
  • FIG. 5 is a side view of a centrifugal container according to an embodiment of the present invention
  • FIG. 6 is a centrifugal container according to an embodiment of the present invention.
  • Figure 7 is a plan view of the main body of the centrifugal container according to an embodiment of the present invention
  • Figure 8 is an exploded perspective view of the body and the valve body of the centrifugal container according to another embodiment of the present invention to be.
  • the centrifugal container 10 is mounted inside the centrifuge in order to separate and recover necessary components from complex fluids, such as separating red blood cells and plasma from blood, or separating stem cells from bone marrow.
  • the first chamber 110 into which the centrifugal target material such as blood or bone marrow is introduced and received, and the second chamber in which the suspended solids centrifuged from the complex fluid injected into the first chamber 110 are decanted and received.
  • a main body 100 comprising a 120; And a lid 200 covering an upper portion of the main body 100 and forming a fluid communication passage P of floating material decanted from the first chamber 110 to the second chamber 120.
  • the first chamber 110 is formed by a cylindrical outer wall 111 whose upper portion is opened and the side and bottom surfaces are sealed, and the centrifugal flows into the first chamber 110 inside the first chamber 110.
  • the valve body 140 is provided by the difference in specific gravity with the material to be separated and positioned in the boundary layer between the materials to be centrifuged.
  • the valve body 140 has a relatively high specific gravity during centrifugal separation of the material to be centrifuged, and thus has a relatively high specific gravity and moves outwardly from the center of rotation, and has a relatively small specific gravity and is relatively close to the center of rotation.
  • the shape is a cup shape with an open top
  • a gap is formed between the outer surface of the valve body 140 and the inner surface of the first chamber 110 so that the floating material can pass therethrough, and the floating material passes through the bottom surface of the valve body 140.
  • a plurality of through holes 141 can be formed.
  • the through hole 141 may be formed at a predetermined interval along the circumferential direction in the form of a central portion and concentric circles of the bottom surface of the valve body 140.
  • the gap formed between the outer surface of the valve body 140 and the inner surface of the first chamber 110 and the plurality of through holes 141 formed on the bottom surface of the valve body 140 are centrifuged floating materials. Silver is preferably passed through, but the precipitate is formed to a size that does not pass.
  • the floating material having a specific gravity smaller than that of the valve body 140 due to the action of the centrifugal force may have a gap between the valve body 140 and the first chamber 110 and a plurality of through holes ( It passes through the 141 and moves to the upper side of the valve body 140, the precipitate material having a specific gravity greater than the valve body 140 is located below the valve body 140.
  • the valve body 140 in order to smoothly move the suspended solids through the plurality of through holes 141 formed on the bottom surface of the valve body 140, the valve body 140 is along the longitudinal direction of the first chamber 110 in the centrifugation process.
  • the linear reciprocating motion is possible, but the rotational motion is prevented, so that the interference between the floating material and the through hole 141 to move upward of the valve body 140 needs to be minimized.
  • the inner side surface of the first chamber 110 and the outer side surface of the valve body 140 are arranged along a direction parallel to the longitudinal direction of the first chamber 110.
  • Each of the plurality of guide ribs 114 and the corresponding number of guide grooves 142 are formed at predetermined angular intervals based on the central axis.
  • the guide ribs 114 are formed on the inner surface of the first chamber 110, the guide grooves 142 are formed on the outer surface of the valve body 140, and are formed at two places with a 180 ° interval. Although shown as an example, the guide rib 114 and the guide groove 142 may be formed in a position opposite to each other, the number of installation may also be configured differently.
  • the guide groove 114a is formed on the inner surface of the first chamber 110, and the guide rib 142a is formed on the outer surface of the valve body 140.
  • the guide grooves 114a and the guide ribs 142a may be formed in three places with a space of 120 °, respectively.
  • the guide grooves 114a and the guide ribs 142a may be formed in four places with a space of 90 °.
  • the installation number be selected in the range of 2-4 places.
  • the second chamber 120 is spaced apart from the outside of the first chamber 110 to surround a portion of the outer wall 111 of the first chamber 110, and is concentric with the outer wall 111 of the first chamber 110. It is configured to include an inner wall 122 and an outer wall 121 forming a structure. The upper end of the inner wall 122 of the second chamber 120 is interconnected by the upper end of the first chamber 110 and the connection portion 112.
  • the second chamber 120 has a shape in which the cross-sectional area is gradually narrowed from the upper end to the lower end, and the lower part of the second chamber 120 makes it easy to take out the suspended substances contained in the second chamber 120.
  • the first bottom portion 123 and the second bottom portion 124 having an inclined shape are gradually narrower in cross section toward the lower portion.
  • the cover 300 has a protrusion 210 covering the upper circumference of the connecting portion 112 in a spaced apart state, and a depression 220 is formed outside the protrusion 210, and recessed with the protrusion 210. Steps 211 are formed at the boundary due to the difference in height between the parts 220.
  • the protrusion 210 has an inlet port 212 for injecting a centrifugal target material such as blood or bone marrow into the first chamber 110 to face the inside of the first chamber 110, the depression
  • the discharge port 222 for extracting the suspended solids centrifuged from the blood or bone marrow to the outside and the air vent 224 for communicating the air inside the main body 100 are formed in the 220. have.
  • the inlet port 212 and the outlet port 222 may be made of a material that can be kept airtight, such as rubber, floating material through the inlet or outlet port 222 of the material to be centrifuged through the inlet port 212 Withdrawal is inserted into the inlet port 212 and the discharge port 222, such as a syringe needle inserted in the centrifugal separation material into the first chamber 110 or decanted by the second chamber 120 is suspended The material is extracted.
  • the space between the connecting portion 112 of the upper end of the outer wall 111 of the first chamber 110 and the stepped portion 211 of the cover 200 is fluid communication between the first chamber 110 and the second chamber 120.
  • the passage P is formed, and the floating material by centrifugation moves to the second chamber 120 through the passage P.
  • the fluid communication passage (P) is configured to be formed over the entire section of the connection portion 112 around the upper end of the first chamber 110, so that the moving path of the centrifuged suspended material is not limited to a specific direction, but the first chamber portion. Since it is formed to be movable through all directions around the upper end of the 110, the suspended solids can move smoothly from the first chamber 110 to the second chamber 120 in the decanting process after centrifugation to precipitate the suspended solids. It can be quickly separated from the material and collected in the second chamber 120.
  • the floating material passing through the connection part 112 located directly above the second chamber 120 is the second chamber 120.
  • the floating material which moves directly inside of and passes through the connection part 112 at a position deviating from the area around the first chamber 110 occupied by the second chamber 120 flows through the guide part 115 and flows into the second part. Guided to the chamber 120 side.
  • the guide portion 115 has a top surface 115a connected to the connection portion 112 on one side of the first chamber 110 opposite to the side where the second chamber 120 is located, and the top surface 115a is positioned.
  • the inclined surface 115b inclined downward in a helical shape along the circumference of the first chamber 110 to both sides of the circumferential direction extends in a symmetrical structure and is connected to both circumferential sides of the second chamber 120. Accordingly, the centrifuged floating material flows through the inclined surface 115b of the guide part 115 after passing through a passage P formed around the upper end of the outer wall 111 of the first chamber 110. Guided to the side), the second chamber 120 is accommodated in the lower cross-sectional area formed narrow.
  • the fastening portion 130 extending in a cam shape in the lower portion of the guide portion 115 is inserted and fixed to the frame (not shown) of the centrifuge and means for mounting the centrifuge container 10 to the centrifuge It serves as.
  • FIG. 9 to 12 is a state diagram showing the centrifugation and decanting process of blood using the centrifuge container according to the present invention.
  • FIG 9 illustrates a state after the blood 300, which is a material for centrifugal separation, is injected into the first chamber 110.
  • the syringe needle passes through the inlet port 212 to form a first chamber.
  • the blood 300 is injected into the chamber 110.
  • the centrifugal container 10 is mounted in the centrifuge (not shown).
  • the centrifuge When the centrifuge is rotated for a predetermined time by operating a centrifuge to separate the red blood cells 301 and the plasma 302 from the blood 300, the blood 300 as shown in FIG. Due to the difference in centrifugal force and specific gravity acting, the blood 300 is centrifuged into the red blood cells 301 and the plasma 302, and the lower side of the valve body 140 (the right side in the drawing) is located in the lower portion of the first chamber 110.
  • the red blood cells 301 having a relatively high specific gravity and fluid resistance are precipitated, and the plasma 302 which is a floating material having a relatively low specific gravity and fluid resistance has a plurality of through holes 141 formed on the bottom surface of the valve body 140. It passes through the gap between the outer surface of the valve body 140 and the inner surface of the first chamber 110 to move to the upper side (left side in the drawing) of the valve body 140.
  • the valve body 140 is rotated by the guide rib 114 and the guide groove 142 provided on the outer surface of the valve body 140 and the inner surface of the first chamber 110.
  • the linear reciprocating motion is performed along the longitudinal direction of the first chamber 110, and the plasma 302 centrifuged from the blood 300 is formed in a plurality of through holes 141 on the bottom surface of the valve body 140. And, it passes through the gap between the valve body 140 and the first chamber 110 can be moved smoothly to the upper side of the valve body 140 to enable rapid centrifugation.
  • the decanting is performed while the rotation speed of the centrifuge is reduced as shown in FIG. 11.
  • the container 10 for centrifugation is inclined at a predetermined angle to one side.
  • the red blood cells 301 and the plasma 302 flow down to the second chamber 120 by their own weight, but the centrifugal force acting in the opposite direction to the flowing direction.
  • the plasma 302, which is a suspended substance, is gradually decanted toward the second chamber 120, thereby preventing the red blood cells 301 from being mixed with the plasma 302 and decanted together.
  • the centrifuge container ( 10) is stopped again in the initial mounting state.
  • the plasma 302 decanted to the second chamber 120 is collected under the second chamber 120 narrowly formed by the first bottom part 123 and the second bottom part 124, and thus, the discharge port 222.
  • the syringe needle through it is possible to easily take out the plasma 302 to the outside.
  • centrifugation and decanting process of blood 300 has been described as an example, but the configuration and operation of the centrifugal container 10 are not limited to this embodiment.
  • Various other complex fluids such as bone marrow, etc., may be applied to the case where the components are recovered by centrifugation.

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Abstract

La présente invention vise à fournir un récipient pour séparation centrifuge permettant une séparation rapide et précise de matières constitutives, lors de la séparation de matières constitutives comportant des fluides physiologiques complexes, tel que le sang et la moelle osseuse, au moyen d'une force centrifuge dérivée de la rotation d'une centrifugeuse et de la différence en poids des matières constitutives. À cet effet, la présente invention concerne un récipient comportant : un corps principal comprenant une première chambre dans laquelle le matériau pour la séparation centrifuge est contenu, et une seconde chambre dans laquelle des solides en suspension qui sont séparés par centrifugation du matériau dans la première chambre sont décantés et reçus, la seconde chambre étant disposée autour de la circonférence extérieure de la première chambre ; et un couvercle, qui recouvre la partie supérieure du corps principal et forme un chemin de communication fluidique (P) entre les première et seconde chambres pour les solides en suspension qui sont décantés.
PCT/KR2012/008959 2011-11-01 2012-10-29 Récipient pour séparation centrifuge permettant une séparation centrifuge rapide WO2013066014A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SG11201403715SA SG11201403715SA (en) 2011-11-01 2012-10-29 Container for centrifugal separation providing rapid centrifugal separation
CN201280060174.6A CN103974777A (zh) 2011-11-01 2012-10-29 可迅速离心分离的离心分离用容器

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110112756A KR101197974B1 (ko) 2011-11-01 2011-11-01 신속한 원심분리가 가능한 원심분리용 용기
KR10-2011-0112756 2011-11-01

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WO2013066014A1 true WO2013066014A1 (fr) 2013-05-10

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KR101897979B1 (ko) * 2017-03-30 2018-09-12 신현순 성분별 분리추출이 용이한 원심분리용기
WO2022158618A1 (fr) * 2021-01-25 2022-07-28 신현순 Ensemble de décantation permettant une séparation et une extraction faciles de constituants

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KR101481539B1 (ko) * 2013-05-15 2015-01-14 (주)어핀텍 원심분리키트
JP6716700B2 (ja) * 2015-12-09 2020-07-01 メディカン カンパニー リミテッドＭｅｄｉｋａｎ Ｃｏ．， Ｌｔｄ． 遠心分離機及び遠心分離方法
CN106075956B (zh) * 2016-05-27 2017-05-17 腾科宝迪（厦门）生物科技有限公司 一种富血小板血浆prp提取离心分离装置及其使用方法
CN106076442B (zh) * 2016-06-07 2017-05-17 腾科宝迪（厦门）生物科技有限公司 一种用于prp多级并行提取的离心瓶及其使用方法
KR101894966B1 (ko) * 2017-03-30 2018-09-04 신현순 원심분리용 용기
KR101874548B1 (ko) * 2017-04-28 2018-07-04 신현순 성분별 분리추출이 용이한 디캔팅키트
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