WO2013066013A1 - Récipient pour la séparation centrifuge - Google Patents

Récipient pour la séparation centrifuge Download PDF

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Publication number
WO2013066013A1
WO2013066013A1 PCT/KR2012/008958 KR2012008958W WO2013066013A1 WO 2013066013 A1 WO2013066013 A1 WO 2013066013A1 KR 2012008958 W KR2012008958 W KR 2012008958W WO 2013066013 A1 WO2013066013 A1 WO 2013066013A1
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WO
WIPO (PCT)
Prior art keywords
chamber
valve body
centrifugal
decanted
cover
Prior art date
Application number
PCT/KR2012/008958
Other languages
English (en)
Korean (ko)
Inventor
박현정
Original Assignee
Park Hyun Jeong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Park Hyun Jeong filed Critical Park Hyun Jeong
Priority to CN201280060192.4A priority Critical patent/CN103974778A/zh
Priority to SG11201403711UA priority patent/SG11201403711UA/en
Publication of WO2013066013A1 publication Critical patent/WO2013066013A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B1/00—Centrifuges with rotary bowls provided with solid jackets for separating predominantly liquid mixtures with or without solid particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Definitions

  • the present invention relates to a centrifuge container mounted on a centrifuge, and more particularly, to accurately classify a layer of a centrifuged material by using a difference in specific gravity and centrifugal force of a physiological complex fluid such as blood or bone marrow.
  • the present invention relates to a centrifugal container for easily recovering a component layer to be obtained.
  • centrifuge is a device that separates materials by using centrifugal force generated when an object rotates.
  • centrifuges are used for the purpose of separating by weight the material mixed with the liquid or cells having a higher specific gravity and adhesion than the liquid. Centrifugation of a complex fluid, such as blood, using a centrifuge divides the fluid into several layers by specific gravity.
  • Blood is used as a major indicator for determining various diseases and health conditions
  • platelets containing abundant growth factors in the blood are used for therapeutic purposes.
  • Blood is composed of red blood cells, white blood cells, platelets, etc.
  • platelets are mainly present in plasma, and plasma is classified into a platelet rich plasma (PRP) layer and a platelet plasma layer (PPP) layer.
  • PRP platelet rich plasma
  • PPP platelet plasma layer
  • PRP concentrated platelets
  • Centrifuges are divided into various types according to the amount of sample to be centrifuged, the rotational speed, and the type of the rotor.
  • the centrifuge accommodates the centrifuge material and separates them into layers by the difference in specific gravity due to centrifugal force. And a plurality of chambers for decanting and recovering a portion of the separated layer.
  • Patent No. 10-0435264 discloses a plurality of chambers for receiving a material to be centrifuged, means for rotating the chambers for centrifuging the material, and a first chamber of the chambers. Disclosed is a centrifuge comprising means for securing the chambers to a predetermined position to discharge the suspended solids to the second chamber.
  • the sample vessel can be fixed in the selected direction during centrifugation or after centrifugation to separate and discharge the float between two or more chambers of the vessel, but the first chamber and the second chamber are arranged side by side adjacent to each other.
  • the fluid communication between the chambers is made only through the bridge formed in the lid, so that after the centrifugation of the container is stopped, the floating material can be poured along with the other chambers, but the container is rotating for the centrifugation.
  • the present invention has been made in order to solve the above problems, the component material in the separation of the component materials of different specific gravity constituting the physiological complex fluid, such as blood or bone marrow using a centrifugal force by the rotation of the centrifuge It is an object of the present invention to provide a container for centrifugation that allows for accurate separation and rapid and easy recovery of a component layer to be obtained.
  • the first chamber 110 is a centrifugation target material is accommodated, and the floating material centrifuged from the material in the first chamber 110 is A main body (100) received and decanted from the first chamber (110) and including a second chamber (120) positioned around an outer circumference of the first chamber (110); A cover 200 covering an upper portion of the main body 100 and forming a fluid communication passage P of the decanted suspended material between the first chamber 110 and the second chamber 120.
  • the second chamber surrounds the entire outer surface of the first chamber 110 to guide the decanted floating material to the second chamber 120 in communication with the passage P. Characterized in that the guide portion 115 formed to be inclined toward the (120) side.
  • an upper end portion of the outer wall 111 of the first chamber 110 is provided with a connecting portion 112 for forming the passage P, and the cover 200 is spaced apart from an upper circumference of the connecting portion 112.
  • a protrusion 210 covering the state, and the depression 220 is formed outside the protrusion 210, the passage (P) is the connection portion 112, the protrusion 210 and the depression 220 It is characterized in that formed in the upper circumference of the first chamber 110 by the spaced space between the stepped 211 formed in the boundary of the.
  • the bottom surface of the guide portion 115 is spaced apart from the recessed portion 220 of the cover 200 to one side of the first chamber 110 across the side where the second chamber 120 is located.
  • the upper surface 115a may be positioned, and the inclined surface 115b along the circumference of the first chamber 110 may extend from the upper surface 115a to both sides thereof, and may be connected to the second chamber 120.
  • connection part 112 may be connected to an upper end of the inner wall 122 of the second chamber 120, and the remaining part of the connection part 112 may be configured to be connected to the guide part 115.
  • the inclined surface 115b has a helical shape and may be installed in a symmetrical shape around the first chamber 110 with respect to the upper surface 115a.
  • first chamber 110 may have a cylindrical shape
  • second chamber 120 may have a shape in which a cross-sectional area is gradually narrowed from an upper end to a lower end.
  • the protrusion 210 of the cover 200 is formed with an inlet port 212 for injecting the centrifugation target material into the first chamber 110, the depression 220 of the cover 200 A discharge port 222 for taking out the decanted material into the second chamber 120 may be formed.
  • the valve body 140 is disposed to be movable along the longitudinal direction of the first chamber (110) so as to be located in the boundary layer between the centrifuged material; In the centrifugal separation process, the suspended solids are separated through the opening 141 formed in the center of the bottom surface of the valve body 140 and at the same time the outer circumferential surface of the valve body 140 and the first chamber 110 are separated from each other. Moving through a gap formed between inner circumferential surfaces; In the decanting process, the valve body 140 of the first chamber 110 is formed through a gap between the valve body 140 and the first chamber 110 which are inclined to one side based on the central axis of the first chamber 110. It may be configured to be guided by the air pressure flowing into the lower side to guide the suspended solids to the second chamber 120 side.
  • centrifugal container According to the centrifugal container according to the present invention, to smoothly guide the floating material toward the second chamber side between the first chamber into which the material to be centrifuged and the second chamber in which the centrifuged floating material is separated from the sediment and accommodated.
  • the fluid communication passage and the guide portion along the entire circumference of the first chamber, there is an effect that can quickly and easily separate the suspended matter from the precipitate.
  • the material centrifuged by the action of the valve body provided inside the first chamber is automatically decanted to the second chamber side, and the cross-sectional shape of the second chamber is formed to be narrower and inclined toward the lower side so that the floating material is formed. There is an effect that can be easily recovered.
  • FIG. 1 is a perspective view of a container for centrifugation according to the present invention
  • FIG. 2 is a cross-sectional view taken along line A-A of FIG.
  • FIG. 3 is an exploded perspective view of a container for centrifugation according to the present invention.
  • FIG. 4 is a front view of the container for centrifugation according to the present invention.
  • FIG. 5 is a side view of the container for centrifugation according to the present invention.
  • FIG. 6 is a rear view of the container for centrifugation according to the present invention.
  • FIG. 7 is a plan view of the main body of the container for centrifugation according to the present invention.
  • FIG. 8 to 11 is a state diagram showing a centrifugation process of blood using a centrifugal container according to the present invention.
  • centrifuge container 100 main body
  • first chamber 111 outer wall
  • connecting portion 115 guide portion
  • valve body 141 opening
  • step 212 inlet port
  • red blood cells 302 plasma
  • FIG. 1 is a perspective view of a centrifugal container according to the present invention
  • FIG. 2 is a sectional view taken along line AA of FIG. 1
  • FIG. 3 is an exploded perspective view of a centrifugal container according to the present invention
  • FIG. 5 is a side view of the centrifugal container according to the present invention
  • FIG. 6 is a rear view of the centrifugal container according to the present invention
  • FIG. 7 is a plan view of the main body of the centrifugal container according to the present invention.
  • the centrifugal container 10 is mounted inside the centrifuge to separate and extract necessary components from the complex fluid, such as when separating red blood cells and plasma from blood, or separating stem cells from bone marrow.
  • the first chamber 110 into which the centrifugal target material such as blood or bone marrow is introduced and received, and the second chamber in which the suspended solids centrifuged from the complex fluid injected into the first chamber 110 are decanted and received.
  • a main body (100) including a guide portion (115) for guiding the suspended material decanted from the first chamber (110) to the second chamber (120) side; And a lid 200 covering an upper portion of the main body 100 and forming a fluid communication passage P of floating material decanted from the first chamber 110 to the second chamber 120.
  • the first chamber 110 is formed by a cylindrical outer wall 111 whose upper portion is opened and the side and bottom surfaces are sealed, and the centrifugal flows into the first chamber 110 inside the first chamber 110.
  • a cup-shaped valve body 140 is provided that is supported by a difference in specific gravity from the material to be separated and is movable in the longitudinal direction of the first chamber 110 so as to be located at the boundary layer between the materials to be centrifuged.
  • the second chamber 120 is spaced apart from the outside of the first chamber 110 to surround a portion of the outer wall 111 of the first chamber 110, and is concentric with the outer wall 111 of the first chamber 110.
  • An inner wall 122 and an outer wall 121 forming a structure are included.
  • the upper end of the inner wall 122 of the second chamber 120 is interconnected by the upper end of the first chamber 110 and the connection portion 112.
  • the second chamber 120 has a shape in which the cross-sectional area is gradually narrowed from the upper end to the lower end, and the lower part of the second chamber 120 makes it easy to take out the suspended substances contained in the second chamber 120.
  • the inclined first bottom portion 123 and the second bottom portion 124 have a narrow cross-sectional area.
  • the cover 200 has a protrusion 210 covering the upper circumference of the connecting portion 112 in a spaced apart state, and a depression 220 is formed outside the protrusion 210, and recessed with the protrusion 210. Steps 211 are formed at the boundary due to the difference in height between the parts 220.
  • the protrusion 210 has an inlet port 212 for injecting a centrifugal target material such as blood or bone marrow into the first chamber 110 to face the inside of the first chamber 110, the depression
  • the discharge port 222 for extracting the suspended solids centrifuged from the blood or bone marrow to the outside and the air vent 224 for communicating the air inside the main body 100 are formed in the 220. have.
  • the inlet port 212 and the outlet port 222 may be made of a material capable of sealing, such as rubber, floating material through the inlet or outlet port 222 of the material to be centrifuged through the inlet port 212 Withdrawal is inserted into the inlet port 212 and the discharge port 222, such as a syringe needle inserted in the centrifugal separation material into the first chamber 110 or decanted by the second chamber 120 is suspended The material is extracted.
  • a material capable of sealing such as rubber, floating material through the inlet or outlet port 222 of the material to be centrifuged through the inlet port 212
  • Withdrawal is inserted into the inlet port 212 and the discharge port 222, such as a syringe needle inserted in the centrifugal separation material into the first chamber 110 or decanted by the second chamber 120 is suspended
  • the material is extracted.
  • the space between the connecting portion 112 of the upper end of the outer wall 111 of the first chamber 110 and the step 211 of the cover 200 is a fluid between the first chamber 110 and the second chamber 120. Forming a communication passage (P), the suspended solids by centrifugal movement to the second chamber 120 through the passage (P).
  • the fluid communication passage (P) is configured to be formed over the entire section of the connection portion 112 around the upper end of the first chamber 110, so that the moving path of the centrifuged floating material is not limited to a specific direction but in all directions. Since it is formed to be movable, it is possible to move smoothly from the first chamber 110 to the second chamber 120 in the decanting process after centrifugation to quickly separate the suspended matter from the sedimentation material and collect it in the second chamber 120. It becomes possible.
  • the floating material passing through the connection part 112 located directly above the second chamber 120 is the second chamber 120. It will be moved directly to the inside of. Floating material passing through the connection portion 112 in a position deviating from the area around the first chamber 110 occupied by the second chamber 120 flows through the guide portion 115 and is led to the second chamber 120 side.
  • the guide portion 115 has a top surface 115a connected to the connection portion 112 to one side of the first chamber 110 across the side where the second chamber 120 is located, the The inclined surface 115b inclined downward in a helical shape along the circumference of the first chamber 110 on both sides of the circumferential direction with respect to the top surface 115a extends in a symmetrical structure so that the circumferential sides of the second chamber 120 Is connected to. Accordingly, the suspended solids generated by centrifugation flows through the inclined surface 115b of the guide 115 after passing through the passage P formed entirely around the upper end of the outer wall 111 of the first chamber 110. Guided to the chamber 120 side, the second chamber 120 is accommodated in the lower cross-sectional area formed narrow.
  • the fastening part 130 extending in a cam shape to the lower part of the guide part 115 is inserted into and fixed to a frame (not shown) of the centrifuge, and serves as a means for mounting the centrifuge container 10 to the centrifuge. do.
  • the valve body 140 provided inside the first chamber 110 may be configured to separate the precipitate and the floating material during centrifugation of the centrifugal separation material to prevent the precipitate from being discharged together with the floating material during the decanting of the floating material.
  • the upper portion has an open cup shape, and an opening portion 141 is formed at the center of the bottom surface of the valve body 140, and is formed between the outer surface of the valve body 140 and the inner surface of the first chamber 110.
  • a gap is formed, and the floating material having a specific gravity smaller than that of the valve body 140 in the centrifugal separation process passes through the opening 141 and the gap to move upwardly of the valve body 140, and has a specific gravity higher than that of the valve body 140. Large deposits are located below the valve body 140.
  • FIG. 8 to 11 is a state diagram showing a centrifugation process of blood using a centrifugal container according to the present invention.
  • the centrifugal container 10 is mounted in the centrifuge (not shown).
  • the centrifuge When the centrifuge is rotated for a predetermined time by operating a centrifuge to separate the red blood cells 301 and the plasma 302 from the blood 300, the blood 300 as shown in FIG.
  • the blood 300 By the centrifugal force acting, the blood 300 is centrifuged into the red blood cells 301 and the plasma 302, and the specific gravity and the fluid resistance are below the first chamber 110 to the lower side (the right side in the drawing) of the valve body 140.
  • the relatively large red blood cells 301 are precipitated, and the plasma 302, which is a floating material having a relatively low specific gravity and fluid resistance, has an opening 141 formed on the bottom surface of the valve body 140 and the valve body 140. Passing through the gap between the outer surface and the inner surface of the first chamber 110 is moved to the upper side (left side in the drawing) of the valve body 140.
  • the rotation speed of the centrifuge is reduced, as shown in FIG. 10.
  • the red blood cells 301 deposited on the lower portion of the valve body 140 exert a pressure on the bottom surface of the valve body 140 such that the valve body 140 is formed in the first chamber ( It is inclined to one side based on the central axis of the 110, the air flows into the lower portion of the first chamber 110 through the gap between the valve body 140 and the first chamber 110, the bottom of the valve body 140
  • the pressure is further acted on the surface by the introduced air pressure, thereby the valve body 140 is pushed to the upper side (left side in the drawing), and the level of the red blood cells 301 is lowered.
  • a portion of the plasma 302 which is a floating material on the upper portion of the valve body 140, flows directly into the second chamber 120 disposed below by weight, and the other portion of the plasma 302 is directly introduced into the main body ( After passing through the fluid communication passage (P) formed between the 100 and the cover 200 flows through the guide portion 115 is collected to the second chamber 120 side.
  • the level of the red blood cells 301 is further lowered due to the inflow of air, and the valve body 140 is further pushed upward. Most of the plasma 302 is decanted and collected toward the second chamber 120, and the plasma 302 collected in the second chamber 120 can be easily taken out through the discharge port 222.
  • the present invention is not limited thereto, and the present invention is not limited thereto. All can be applied.

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Abstract

La présente invention vise à fournir un récipient pour la séparation centrifuge, permettant une séparation précise de matières constitutives et d'obtenir une collecte rapide et facile d'une couche de composants, lors de la séparation de matières constitutives de différents poids qui comportent des fluides physiologiques complexes, tel que le sang ou la moelle osseuse, au moyen de la force centrifuge provenant des rotations d'une centrifugeuse. À cet effet la présente invention concernent un récipient comportant : un corps principal comprenant une première chambre dans laquelle un matériau pour la séparation centrifuge est contenu, et une seconde chambre dans laquelle des solides en suspension qui sont séparés par centrifugation depuis le matériau dans la première chambre sont décantés et reçus, la seconde chambre étant disposée autour de la circonférence extérieure de la première chambre ; et un couvercle qui recouvre la partie supérieure du corps principal et forme un chemin de communication fluidique (P) entre les première et seconde chambres pour les solides en suspension qui sont décantés.
PCT/KR2012/008958 2011-10-31 2012-10-29 Récipient pour la séparation centrifuge WO2013066013A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201280060192.4A CN103974778A (zh) 2011-10-31 2012-10-29 离心分离用容器
SG11201403711UA SG11201403711UA (en) 2011-10-31 2012-10-29 Container for centrifugal separation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0111847 2011-10-31
KR1020110111847A KR101197908B1 (ko) 2011-10-31 2011-10-31 원심분리용 용기

Publications (1)

Publication Number Publication Date
WO2013066013A1 true WO2013066013A1 (fr) 2013-05-10

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Application Number Title Priority Date Filing Date
PCT/KR2012/008958 WO2013066013A1 (fr) 2011-10-31 2012-10-29 Récipient pour la séparation centrifuge

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KR (1) KR101197908B1 (fr)
CN (1) CN103974778A (fr)
SG (1) SG11201403711UA (fr)
WO (1) WO2013066013A1 (fr)

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WO2015021189A1 (fr) 2013-08-06 2015-02-12 Regenerative Sciences, Llc Dispositif et procédés d'isolement de partie adipeuse de moelle osseuse
WO2018155764A1 (fr) * 2017-02-27 2018-08-30 (주)어핀텍 Appareil étanche à l'air destiné à séparer et purifier un extrait
KR101897979B1 (ko) * 2017-03-30 2018-09-12 신현순 성분별 분리추출이 용이한 원심분리용기

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KR101894966B1 (ko) 2017-03-30 2018-09-04 신현순 원심분리용 용기
KR101874548B1 (ko) 2017-04-28 2018-07-04 신현순 성분별 분리추출이 용이한 디캔팅키트
KR102271814B1 (ko) * 2018-01-09 2021-06-30 신현순 원심분리용기용 성분분리 플로터
KR102051207B1 (ko) * 2018-01-30 2019-12-03 이준석 원심분리용 피스톤
KR102407040B1 (ko) 2020-05-22 2022-06-08 황의재 원심분리용 용기

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US4026433A (en) * 1975-01-16 1977-05-31 Egidia Crippa Container provided with an outer testpiece for the analysis of urine and other acid liquids
KR100435264B1 (ko) * 1995-05-05 2004-07-31 하베스트 테크놀로지스 코포레이션 자동멀티-디갠팅을이용한원심분리기,성분분리방법및침전물분리장치
US20110160031A1 (en) * 2002-08-02 2011-06-30 Harvest Technologies Corporation Decanting centrifuge with vibration isolation
JP2008284339A (ja) * 2007-05-21 2008-11-27 Samsung Kwangju Electronics Co Ltd 真空掃除機用サイクロン集塵ユニット

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015021189A1 (fr) 2013-08-06 2015-02-12 Regenerative Sciences, Llc Dispositif et procédés d'isolement de partie adipeuse de moelle osseuse
KR20160039565A (ko) * 2013-08-06 2016-04-11 리제너러티브 사이언시즈, 엘엘씨 골수 지방 부분 분리 장치 및 방법
JP2016533811A (ja) * 2013-08-06 2016-11-04 リジェネレイティブ サイエンシーズ， エルエルシー 骨髄脂肪部分単離のデバイスおよび方法
EP3030279A4 (fr) * 2013-08-06 2017-03-08 Regenerative Sciences, LLC Dispositif et procédés d'isolement de partie adipeuse de moelle osseuse
US9976115B2 (en) 2013-08-06 2018-05-22 Regenexx, LLC Bone marrow adipose portion isolation device and methods
US10537596B2 (en) 2013-08-06 2020-01-21 Regenexx Llc Bone marrow adipose portion isolation device and methods
KR102349673B1 (ko) * 2013-08-06 2022-01-11 리제넥스 엘엘씨 골수 지방 부분 분리 장치 및 방법
WO2018155764A1 (fr) * 2017-02-27 2018-08-30 (주)어핀텍 Appareil étanche à l'air destiné à séparer et purifier un extrait
KR101897979B1 (ko) * 2017-03-30 2018-09-12 신현순 성분별 분리추출이 용이한 원심분리용기

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