CN106075956B - Platelet-rich blood plasma PRP extraction and centrifugation apparatus and use method thereof - Google Patents
Platelet-rich blood plasma PRP extraction and centrifugation apparatus and use method thereof Download PDFInfo
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- CN106075956B CN106075956B CN201610365011.9A CN201610365011A CN106075956B CN 106075956 B CN106075956 B CN 106075956B CN 201610365011 A CN201610365011 A CN 201610365011A CN 106075956 B CN106075956 B CN 106075956B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
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Abstract
The invention provides a PRP extraction and centrifugation apparatus and a use method thereof. The PRP extraction and centrifugation apparatus comprises a first centrifuge bottle and a second centrifuge bottle, the second centrifuge bottle is nested in the first centrifuge bottle in a suspending manner, the first centrifuge bottle and the second centrifuge bottle are connected with a tee pipe coupling through a first non-return valve and a second non-return valve, and the controlled flow directions of the first non-return valve and the second non-return valve are opposite; the bottle cap of the first centrifuge bottle is provided with a centrifuge bottle nesting port and a second conduit port; the centrifuge nest port is positioned in the center of the first centrifuge bottle; a conduit on the second conduit port is connected with the tee pipe coupling through the first non-return valve; the bottle cap of the second centrifuge bottle is provided with a second gas introduction port, a second blood plasma inlet and a third conduit port; and the a conduit at the second blood plasma inlet is connected with the tee pipe coupling through the second non-return valve. The PRP extraction and centrifugation apparatus allows two-stage centrifuge of blood plasma to be carried out without blood transfer, so the blood is prevented from being polluted.
Description
Technical field
The present embodiments relate to technical field of medical instruments, more particularly to a kind of PRP extract centrifugal separating device and its
Using method.
Background technology
Platelet rich plasma (Platelet Rich Plasma, abbreviation PRP) is by containing height obtained from centrifugal blood
The thrombocyte plasma of concentration.The blood platelet of human body also contains in addition to it can provide cohesion when hemostasis in blood platelet
Have much relevant with a wound healing and bone regeneration various growth factors, such as platelet derived growth factor, conversion growth because
Son, IGF, EGF and VEGF etc..Growth factor in blood platelet,
Differentiation and propagation to cell plays facilitation, and these growth factors have each other cooperative response, thin with other promotions
The factor interaction of cytoactive and impact, maintain the balance of organizational environment jointly, have to wound healing, reparation and regeneration
Important effect.Further, since PRP can be extracted from patient's self-blood, after extracted enrichment is processed, can be used for related disease
The treatment of disease, thus abundance, draw materials conveniently, and preparation method is simple and body can be allowed to absorb.Itself PRP is using can keep away
The problem for exempting from viral transmission and immunological rejection occurs.Therefore PRP is widely applied in different medical domains.Such as orthopaedics, mouth
Chamber section, decorative sursery, sports medical science and aesthetic medicine.
At present, specialty is there is no to be used for the detached equipment listing of extraction of PRP, mostly using open manual secondary centrifuging
Method prepares PRP, but the following shortcoming for existing strongly limit it in clinical application：1. outside contamination, Duo Gerong are easily subject to
Shift in device, increased platelet activation and contaminated chance；2. recovery rate of blood platelet is relatively low；3. easily by the shadow of operation factors
Ring, preparation method is not sufficiently stable；4. without special application tool, in-convenience in use.
The content of the invention
For solve problem, the present invention provides a kind of platelet rich plasma PRP and extracts centrifugal separating device, including first is centrifuged
Bottle and the second centrifugal bottle, second centrifugal bottle is vacantly nested in first centrifugal bottle, first centrifugal bottle and described
Second centrifugal bottle is connected by tee pipe coupling, and the first check valve and the second check valve, institute are provided with the tee pipe coupling
The control flow direction for stating the first check valve and second check valve is contrary；
The first centrifugation bottle cap is provided with the first blow vent, centrifugal bottle nesting mouth, the first blood plasma inlet, first leads
The mouth of pipe and the second conduit mouth；Centrifugal bottle nesting mouth is located at the center of first centrifugal bottle, for it is nested place second from
Heart bottle；Conduit in second conduit mouth is connected by the first check valve with tee pipe coupling conducting；
The second centrifugation bottle cap is provided with the second blow vent, the second blood plasma inlet and the 3rd conduit mouth；Described
The conduit of two blood plasma inlets is connected by the second check valve with tee pipe coupling conducting.
Further, first and second check valve is provided with liquid feeding end and outlet end；The liquid feeding end with it is described go out liquid
End is isolated by funnel-form interlayer, and the beaker flask of the funnel-form interlayer is located at the outlet end；The beaker flask bottom is provided with logical
Hole, makes the liquid feeding end be connected with the outlet end；The outlet end is provided with screen pack, before the funnel-form interlayer
Side；Be provided with lightweight bead between the beaker flask and the screen pack, the lightweight bead it is straight with diameter greater than the through hole
Footpath；
The liquid feeding end of first check valve is connected with the conduit in second conduit mouth；First check valve goes out
Liquid end is connected with the first interface of the tee pipe coupling；
The liquid feeding end of second check valve is connected with the second interface of the tee pipe coupling；Second check valve
Outlet end is connected with the second blood plasma inlet.
Further, the conduit, between check valve and tee pipe coupling using flexible pipe connect.
Further, first centrifugal bottle and the second centrifugal bottle bottom are the coniform of closing.
Further, the 3rd interface of the tee pipe coupling is provided with storage area ball.
Further, the centrifugal bottle nesting mouth, the first conduit mouth, the second conduit mouth and the 3rd conduit mouth are provided with close
Envelope rubber ring.
The present invention also provides the using method that a kind of any one PRP as mentioned above extracts centrifugal separating device, including：
Will containing anti-coagulants new blood from the first blood plasma inlet inject the first centrifugal bottle in, by the PRP extract from
Heart separator is placed in a centrifuge carries out first time centrifugation；
After the completion of being centrifuged for the first time, blood completes layering, is extracted by the conduit of the first conduit mouth using syringe and is in
The red blood cell layer of bottom；
When syringe extract to red blood cell layer with the PRP lines of demarcation in intermediate layer when, using syringe by the
The conduit of two conduit mouths extracts PRP layer liquid, until extracting to PRP layers and on the supernatant layer line of demarcation on upper strata；
PRP layers liquid in syringe is injected in the second centrifugal bottle by the second blood plasma inlet, by PRP extract from
Heart separator is again placed in carrying out second centrifugation in centrifuge；
After the completion of secondary centrifuging, the bottom red blood cell layer in the second centrifugal bottle is extracted with syringe；
When syringe is extracted to red blood cell layer and PRP layer lines of demarcation, exchange new syringe for and received by the 3rd conduit mouth
Collect the PRP layers in the second centrifugal bottle.
Further, after the completion of being centrifuged for the first time, PRP layer liquid is extracted by the conduit of the second conduit mouth using syringe
When injecting to the second centrifugal bottle, contain anti-coagulants new blood to the injection of the first centrifugal bottle again.
Further, collect secondary centrifuging after the second centrifugal bottle in PRP layers when, can take out according to actual needs
Different amounts of clear liquid is taken, variable concentrations PRP is mixedly configured into.
The PRP provided using the present invention extracts centrifugal separating device, and secondary centrifuging can be carried out to blood plasma, and is carrying out two
During secondary centrifugation, without the need for blood is transferred to outside centrifugal bottle, directly shifted inside device, will not be contaminated；It is another
Aspect, second when being centrifuged is carried out in less centrifugal bottle, and PRP layers are obvious with the line of demarcation of red blood cell layer, easily take
Go out rich platelet blood.The PRP provided using the present invention extracts the using method of centrifugal separating device, the rich platelet blood of extraction
Slurry concentration is high, will not be contaminated.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are these
Some bright embodiments, for those of ordinary skill in the art, without having to pay creative labor, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 extracts centrifugal separating device structural representation for the PRP that the present invention is provided；
Fig. 2 is the first centrifugal bottle Bottle cap structure schematic diagram in Fig. 1；
Fig. 3 is the second centrifugal bottle Bottle cap structure schematic diagram in Fig. 1；
Fig. 4 is one-way valve structures schematic diagram in Fig. 1；
Fig. 5 is filter screen structure schematic diagram in Fig. 4；
Fig. 6 A are the schematic diagram that check valve is extracted in centrifugal separating device use state one in PRP；
Fig. 6 B are the schematic diagram that check valve is extracted in centrifugal separating device use state two in PRP；
Fig. 7 is another preferred embodiment schematic diagram of tee pipe coupling in Fig. 1；
Fig. 8 is the layering schematic diagram of blood after centrifugation.
Reference：
The tee pipe coupling of 10 first 20 second centrifugal bottle of centrifugal bottle 30
The blow vent of 41 first check valve, 42 second check valve 101 first
The conduit mouth of 102 centrifugal bottles nesting mouth, 103 first blood plasma inlet 104 first
The blood plasma blow vent of 105 second conduit mouth, 201 second blow vent 202 second
The second interface of 203 the 3rd conduit mouth, 301 first interface 302
The liquid feeding end of 303 the 3rd interface, 304 storage area ball 401
The through hole of 402 outlet end, 403 beaker flask 404
The lightweight bead of 405 screen pack 406
Specific embodiment
To make purpose, technical scheme and the advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
The a part of embodiment of the present invention, rather than the embodiment of whole.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
The embodiment of the present invention provides a kind of PRP and extracts centrifugal separating device, and referring to Fig. 1~Fig. 3, the PRP extracts centrifugation point
Include the first centrifugal bottle 10 and the second centrifugal bottle 20 from device, second centrifugal bottle 20 is vacantly nested in first centrifugal bottle
In 10, first centrifugal bottle 10 is connected with second centrifugal bottle 20 by tee pipe coupling 30, in the tee pipe coupling
30 controlling streams for being provided with the first check valve 41 and the second check valve 42, first check valve 41 and second check valve 42
To contrary；
As shown in Fig. 2 the bottle cap of the first centrifugal bottle 10 is provided with the first blow vent 101, centrifugal bottle nesting mouth 102, the first blood
Slurry inlet 103, the first conduit mouth 104 and the second conduit mouth 105；
Wherein：The effect of first blow vent 101 is the effect that air pressure is adjusted when extracting and inject liquid；
Centrifugal bottle nesting mouth 102 is used to place the second centrifugal bottle 20；
First blood plasma inlet 103 is used to new blood is injected in the first centrifugal bottle 10；
Conduit extends to the bottom of the first centrifugal bottle 10 through the first conduit mouth 104 and the second conduit mouth 105, and first
The conduit of conduit mouth 104 is used to extract the red blood cell layer of the bottom of the first centrifugal bottle 10 after the completion of centrifugation；Second conduit mouth 105
Conduit is used to extract the PRP (conduit of the first conduit mouth 104 is extracted after red blood cell layer) in remaining intermediate layer, and by three-way pipe
Joint 30 is delivered in the second centrifugal bottle 20.
As shown in figure 3, the bottle cap of the second centrifugal bottle 20 is provided with the second blow vent 201, the second blood plasma inlet 202 and the 3rd
Conduit mouth 203, wherein：
The effect of second blow vent 201 is the effect that air pressure is adjusted when extracting and inject liquid；
The conduit of the second blood plasma inlet 202 by the tee pipe coupling 30 conducting connect, for input from first from
The PRP that heart bottle 10 is extracted；
Conduit passes through the 3rd conduit mouth 203, and extends to the bottom of the second centrifugal bottle 20, for extracting centrifugation after second from
The red blood cell layer of the bottom of heart bottle 20.
When being embodied as, the check valve in above-described embodiment can be as shown in Figure 4：First check valve 41 and the second check valve
42 are provided with liquid feeding end 401 and outlet end 402, and liquid feeding end 401 is isolated with outlet end 402 by funnel-form interlayer.Funnel-form interlayer
Beaker flask 403 is located at outlet end 402.The bottom of beaker flask 403 is provided with through hole 404, makes liquid feeding end 401 be connected with outlet end 402.
Outlet end 402 is provided with screen pack 405 (as shown in Figure 5), positioned at funnel-form interlayer front；Between beaker flask 403 and screen pack 405
It is provided with lightweight bead 406, the diameter with diameter greater than through hole 404 of lightweight bead 406.When air pressure or hydraulic pressure are from outlet end 402
When putting on beaker flask 403, lightweight bead 406 is adjacent to through hole 404, and liquid or gas will not flow out from through hole 404.Work as air pressure
Or hydraulic pressure from liquid feeding end 401 put on through hole 404 when, lightweight bead 406 can float, and no longer be adjacent to through hole 404, liquid or
Gas can enter outlet end 402 from through hole 404.The one side of screen pack 405 is used to prevent lightweight bead 406 by gas or liquid
Body is rushed to check valve, and on the other hand, liquid or gas smoothly screen pack 405 can pass through.
Conduit in second conduit mouth 105 is connected with the liquid feeding end 401 of the first check valve 41, and first check valve 41 goes out liquid
End 402 is connected with the first interface 301 of tee pipe coupling 30.The outlet end of the second blood plasma inlet 202 and the second check valve 42
402 connections, the liquid feeding end 401 of the second check valve 42 is connected with the second interface 302 of tee pipe coupling 30.
When being embodied as, when three interface 303 with syringe connecting tee pipe joint 30, by the second conduit mouth
When conduit on 105 extracts the blood inside the first centrifugal bottle 10, the lightweight bead 406 in the first check valve 41 can float, no
Through hole 404 is adjacent to again, and at the same time, the lightweight bead 406 of the second check valve 42 can be held is adjacent to through hole 404, and blood will not
Second centrifugal bottle 20 is entered by the second check valve 42, therefore, blood can only pass through the first check valve 41 from the first check valve 41
And tee pipe coupling 30 enters syringe inside (as shown in Figure 6A).
When the outside injecting blood of syringe, the lightweight bead 406 of the first check valve 41 compacted can be adjacent to through hole 404,
Blood will not enter the first centrifugal bottle 10 by the first check valve 51, and at the same time, blood is pressed downward the light of the second check valve 42
Matter bead 406, lightweight bead 406 is no longer adjacent to through hole 404, and blood can only enter the second centrifugal bottle 20 from the second check valve 42
(as shown in Figure 6B).
Preferably, the conduit and the first check valve 41 in the second conduit mouth 105, the first check valve 41 and tee pipe coupling
30, the check valve 42 of tee pipe coupling 30 and second is connected between second check valve 42 and the second blood plasma inlet 202 using flexible pipe
Connect.Using flexible pipe connecting conduit, check valve and tee pipe coupling 30, easy to operate, good airproof performance will not leakage.
It is preferred that the first centrifugal bottle 10 and the bottom of the second centrifugal bottle 20 be it is coniform.It is coniform using coniform bottom
Bottom causes bottom liquid relatively to concentrate, and when the blood inside centrifugal bottle is extracted, can more completely extract inside centrifugal bottle
Blood.
It is preferred that the 3rd interface 303 of tee pipe coupling 30 is provided with storage area ball 304 (as shown in Figure 7), storage area
Ball 304 is easy to temporarily deposit blood.Because PRP is typically less, storage area ball can be arranged according to the specification of the first centrifugal bottle
Volume size, is advisable with reaching the detached PRP of the first centrifugal bottle of storage, and so PRP can be drawn in syringe,
Only need to be cached to storage area ball.
It is preferred that conduit, centrifugal bottle, check valve, tee pipe coupling 30 and storage empty 304 are made using transparent material,
It is easy to the blood situation of real-time monitored device interior.
It is preferred that centrifugal bottle nesting mouth 102, the first conduit mouth 104, the second conduit mouth 105 and the 3rd conduit mouth 203 are equal
It is provided with sealing rubber ring.Each conduit mouth on centrifugation bottle cap, centrifugal bottle nesting mouth 102 is respectively provided with sealing rubber ring
(medical), is easy to the installation and fixation of conduit and centrifugal bottle, and can reach good sealing effectiveness.
The present invention also provides the using method that a kind of any one PRP as mentioned above extracts centrifugal separating device, concrete real
Shi Shi：
Will containing anti-coagulants new blood from the first blood plasma inlet inject the first centrifugal bottle in, by the PRP extract from
Heart separator is placed in a centrifuge carries out first time centrifugation；
After the completion of being centrifuged for the first time, blood completes layering, is extracted by the conduit of the first conduit mouth using syringe and is in
The red blood cell layer of bottom；As shown in figure 8, being generally divided into three layers after centrifugal blood, bottom is red blood cell layer, and intermediate layer is PRP layers,
The superiors are supernatant layer；Wherein, supernatant layer and the PRP that the line of demarcation of intermediate layer PRP layers is a, red blood cell layer and intermediate layer
The line of demarcation of layer is b；
When syringe is extracted to red blood cell layer and is under the PRP layers line of demarcation b in intermediate layer, passed through using syringe
The conduit of the second conduit mouth extracts PRP layer liquid, until extracting to PRP layers and on the supernatant layer line of demarcation a on upper strata；This
When, the liquid of extraction is just contained and mixes unclear liquid between PRP and PRP and clear liquid, red blood cell；
Extraction night in syringe is injected in the second centrifugal bottle by the second blood plasma inlet, PRP is extracted and is centrifuged
Separator is again placed in carrying out second centrifugation in centrifuge；
After being centrifuged twice, the bottom red blood cell layer in the second centrifugal bottle is extracted with syringe；
When syringe is extracted to red blood cell layer and PRP layer lines of demarcation, exchange new syringe for and received by the 3rd conduit mouth
Collect the PRP layers in the second centrifugal bottle.
Using PRP provided in an embodiment of the present invention extract centrifugal separating device, extract PRP method be compared to it is traditional
Manual collection method, with purity height, collects free of contamination feature, specifically：
Traditional centrifugal bottle general requirements is larger, and PRP layers because amount it is fewer, centrifugation after on centrifugal bottle with it is red
Cell once, supernatant layer boundary it is unclear, it is general to be difficult accurately to extract PRP layers.If by PRP layers and red blood cell layer, clear liquid
Layer boundary is extracted in the lump, will carry red blood cell, causes purity not high；If in order to reach certain purity, avoiding boundary
Extract, then the PRP for extracting can be caused to measure very little, and be not easily controlled；And PRP provided in an embodiment of the present invention extracts centrifugation
Device, fractional centrifugation, when being centrifuged first time, will be including PRP layers and its with red blood cell layer, supernatant layer boundary mixed liquor in the lump
The second centrifugal bottle is input to, it is ensured that all of PRP layers are included, after secondary centrifuging, due to the PRP layers institute in the second centrifugal bottle
Accounting rate is higher, and the second centrifugal bottle, because specification is less, scale is also more apparent, therefore is easy for energy control, extracts pure
The very high PRP of degree；
And PRP provided in an embodiment of the present invention is extracted during centrifugal separating device intermediary operation, due to whole in close
Dustless state is sealed, bacterium infection is not result in；
It is preferred that to the second centrifugal bottle inject extract when, to the first centrifugal bottle injection contain anti-coagulants new blood；
By the PRP extraction centrifugal separating device be placed in a centrifuge, while in the first centrifugal bottle containing anti-coagulants new blood
It is centrifuged with the extract in the second centrifugal bottle, it is possible to achieve concurrent working, is improved extraction efficiency.
It is preferred that collect secondary centrifuging after the second centrifugal bottle in PRP layers when, can according to actual needs, extract
Different amounts of clear liquid, is configured to variable concentrations PRP, meets different demands.
Although more herein used such as the first centrifugal bottle, the second centrifugal bottle, the first check valve, second is unidirectional
Valve, tee pipe coupling, the first blow vent, the first blood plasma inlet, centrifugal bottle nesting hole, the second conduit mouth, the second blow vent, the
Two blood plasma blow vents, the 3rd conduit mouth, first interface, second interface, the 3rd interface, storage area ball, liquid feeding end, outlet end,
The terms such as beaker flask, through hole, screen pack, lightweight bead, but be not precluded from using the possibility of other terms.Using these terms
Just for the sake of more easily describing and explaining the essence of the present invention；Be construed as any additional restriction be all with
Spirit of the invention is contrary.
Finally it should be noted that：Various embodiments above only to illustrate technical scheme, rather than a limitation；To the greatest extent
Pipe has been described in detail with reference to foregoing embodiments to the present invention, it will be understood by those within the art that：Its according to
So the technical scheme described in foregoing embodiments can be modified, either which part or all technical characteristic are entered
Row equivalent；And these modifications or replacement, do not make the essence disengaging various embodiments of the present invention technology of appropriate technical solution
The scope of scheme.
Claims (9)
1. a kind of platelet rich plasma PRP extracts centrifugal separating device, it is characterised in that：Including the first centrifugal bottle (10) and second
Centrifugal bottle (20), second centrifugal bottle (20) is vacantly nested in first centrifugal bottle (10), first centrifugal bottle
(10) connected by tee pipe coupling (30) with second centrifugal bottle (20), in the tee pipe coupling (30) first is provided with
Check valve (41) and the second check valve (42), the control of first check valve (41) and second check valve (42) flows to phase
Instead；
First centrifugal bottle (10) bottle cap is provided with the first blow vent (101), centrifugal bottle nesting mouth (102), the first blood plasma note
Entrance (103), the first conduit mouth (104) and the second conduit mouth (105)；Centrifugal bottle nesting mouth (102) is positioned at described first
The center of centrifugal bottle (10), for nested the second centrifugal bottle (20) is placed；Conduit on second conduit mouth (105) is by the
One check valve (41) is connected with the tee pipe coupling (30) conducting；
Second centrifugal bottle (20) bottle cap is provided with the second blow vent (201), the second blood plasma inlet (202) and the 3rd conduit
Mouth (203)；The conduit of the second blood plasma inlet (202) is led by the second check valve (42) with the tee pipe coupling (30)
Lead to and connect.
2. PRP according to claim 1 extracts centrifugal separating device, it is characterised in that：First and second check valve
(41,42) it is provided with liquid feeding end (401) and outlet end (402)；The liquid feeding end (401) is with the outlet end (402) by funnel-form
Interlayer is isolated, and the beaker flask (403) of the funnel-form interlayer is positioned at the outlet end (402)；Beaker flask (403) bottom sets
There is through hole (404), make the liquid feeding end (401) be connected with the outlet end (402)；The outlet end (402) is provided with filtration
Net (405), positioned at funnel-form interlayer front；It is little lightweight to be provided between the beaker flask (403) and the screen pack (405)
Ball (406), the diameter with diameter greater than the through hole (404) of the lightweight bead (406)；
The liquid feeding end (401) of first check valve (41) is connected with the conduit on second conduit mouth (105)；Described first
The outlet end (402) of check valve (41) is connected with the first interface (301) of the tee pipe coupling (30)；
The liquid feeding end (401) of second check valve (42) is connected with the second interface (302) of the tee pipe coupling (30)；Institute
The outlet end (302) for stating the second check valve (42) is connected with the second blood plasma inlet (202).
3. PRP according to claim 1 extracts centrifugal separating device, it is characterised in that：The conduit, check valve and three
Connected using flexible pipe between connection for bbreather pipe.
4. PRP according to claim 1 extracts centrifugal separating device, it is characterised in that：First centrifugal bottle (10) and
Second centrifugal bottle (20) bottom is the coniform of closing.
5. PRP according to claim 1 extracts centrifugal separating device, it is characterised in that：The tee pipe coupling (30)
3rd interface (303) is provided with storage area ball (304).
6. PRP according to claim 1 extracts centrifugal separating device, it is characterised in that：The centrifugal bottle nesting mouth
(102), the first conduit mouth (104), the second conduit mouth (105) and the 3rd conduit mouth (203) are provided with sealing rubber ring.
7. the PRP any one of a kind of employing claim 1~6 extracts the using method of centrifugal separating device, its feature
It is, including：
To inject in the first centrifugal bottle from the first blood plasma inlet containing anti-coagulants new blood, the PRP will be extracted into centrifugation point
Being placed in a centrifuge from device carries out first time centrifugation；
After the completion of being centrifuged for the first time, blood completes layering, is extracted by the conduit of the first conduit mouth using syringe and is in bottom
Red blood cell layer；
When syringe is extracted to red blood cell layer and is under the PRP layers line of demarcation in intermediate layer, using syringe second is passed through
The conduit of conduit mouth extracts PRP layer liquid, until extracting to PRP layers and on the supernatant layer line of demarcation on upper strata；
PRP layers liquid in syringe is injected in the second centrifugal bottle by the second blood plasma inlet, PRP is extracted into centrifugation point
It is again placed in carrying out second centrifugation in centrifuge from device；
After the completion of secondary centrifuging, the bottom red blood cell layer in the second centrifugal bottle is extracted with syringe；
When syringe is extracted to red blood cell layer and during PRP layer lines of demarcation, exchange new syringe for and collect the by the 3rd conduit mouth
PRP layers in two centrifugal bottles.
8. PRP according to claim 7 extracts the using method of centrifugal separating device, it is characterised in that：It is centrifuged for the first time
After the completion of, using syringe by the conduit of the second conduit mouth extract PRP layers liquid to the second centrifugal bottle inject when, again to the
The injection of one centrifugal bottle contains anti-coagulants new blood.
9. PRP according to claim 7 extracts the using method of centrifugal separating device, it is characterised in that：It is secondary collecting
During PRP layers in the second centrifugal bottle after centrifugation, according to actual needs different amounts of clear liquid can be extracted, be mixedly configured into not
With concentration PRP.
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| CN106994396B (en) * | 2017-05-23 | 2019-03-29 | 武汉朗克医疗器械有限公司 | PRP continuous centrifugal separation device and separation method |
| CN110251995B (en) * | 2019-06-17 | 2021-05-14 | 郭钦 | Economical preparation method of platelet-rich plasma |
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