CN210620844U - Active white blood cell collecting device - Google Patents
Active white blood cell collecting device Download PDFInfo
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- CN210620844U CN210620844U CN201921194781.7U CN201921194781U CN210620844U CN 210620844 U CN210620844 U CN 210620844U CN 201921194781 U CN201921194781 U CN 201921194781U CN 210620844 U CN210620844 U CN 210620844U
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Abstract
The utility model relates to an active white blood cell collection device, its characterized in that: collect bag, plasma including centrifugal bag, erythrocyte and collect bag, cell clamp and water conservancy diversion needle, the upper portion of centrifugal bag be provided with the first pipe of going into the blood vessel of connecting the blood taking needle, connecting the erythrocyte and collecting the bag to and be used for connecting the first joint of water conservancy diversion needle, the bottom of centrifugal bag is provided with the second pipe of connecting the plasma and collecting the bag, and the erythrocyte is collected and is provided with the third pipe between bag and the plasma collection bag, all be provided with the switch on going into blood vessel, first pipe, second pipe, the third pipe, the centrifugal bag is close to upper end and lower extreme department and respectively sets up a leucocyte pilot line. The utility model has simple structure and low cost, and can collect a large amount of active leucocytes with little influence on the health of the blood-sampled person.
Description
Technical Field
The present invention relates to a leukocyte collection device in blood, and more particularly to a leukocyte separation structure.
Background
The cellular immunotherapy is widely applied to the tumor treatment, and comprises the steps of collecting human autoimmune cells (mainly leukocytes), increasing the number of the human autoimmune cells by thousands of times through in vitro culture, enhancing the targeting killing function, and then infusing the human autoimmune cells back to a human body to kill pathogens, cancer cells and mutated cells in blood and tissues, break immune tolerance, activate and enhance the immunocompetence of the body, and take the dual effects of treatment and health care into consideration.
In tumor treatment, 50-100ml (by using a syringe) of peripheral blood is conventionally collected in a single time for in vitro target cell culture, but the effect is not ideal, and the original cells are not obtained enough in the single collection. The reason is that the number of white blood cells of a patient is small, and collected red blood cells and blood plasma cannot be returned to the patient, so that a part of blood cells are lost, a large amount of blood can not be collected, and the patient can be injured. In addition, the active leucocytes can also be applied to the development of leucocyte diagnostic reagents.
The existing leukocyte filtering device can separate the leukocytes from the blood, but the separated leukocytes lose activity and are generally treated as medical wastes along with the device. If a method for maintaining the activity of the white blood cells can be found, the comprehensive utilization of blood resources can be a new way.
Peripheral blood is separated into plasma layer, leukocyte and platelet layer, and erythrocyte layer from top to bottom after centrifugation.
Disclosure of Invention
The object of the present invention is to improve and innovate the drawbacks and problems of the background art by providing a simple device capable of collecting large quantities of activated leukocytes and allowing the return of the residual blood.
The technical scheme of the invention is that an active white blood cell collecting device is constructed, the active white blood cell collecting device comprises a centrifugal bag, a red blood cell collecting bag, a plasma collecting bag, a cell clamp and a flow guide needle, wherein the centrifugal bag is provided with a blood inlet pipe connected with a blood taking needle, a first conduit connected with the red blood cell collecting bag is arranged at the upper part of the centrifugal bag, and a first joint used for being connected with the flow guide needle;
the cell clamp is two clamping plates with one ends hinged together, and the cell clamp also comprises a control device for controlling the two clamping plates to open and close;
the guide needle comprises a needle tube with the length of 10-20cm and a second joint which is arranged at one end of the needle tube and is matched with the first joint.
The first connector is a short pipe connected with the interior of the centrifugal bag, an external thread arranged at one end of the short pipe and a first cap screwed on the external thread through threads to seal the short pipe, the second connector is a second cap, and the central opening of the second cap is connected with the needle tube or the second cap and the needle tube are of an integrated structure.
The guide needle is made of high-temperature resistant plastic or metal material.
Preferably, the bottom of the centrifugal bag is provided with a hanging hole.
The method for collecting the white blood cells by the active white blood cell collecting device comprises the following steps:
the free end of the blood inlet pipe is connected with a blood taking needle, a corresponding switch is opened, blood is drained into the centrifugal bag, and sterile heat sealing cutting is carried out on the blood inlet pipe after the required amount of blood is collected;
placing the centrifugal bag, the erythrocyte collecting bag and the plasma collecting bag into a centrifuge for centrifugal operation to separate blood into layers;
inverting the centrifuged centrifugation bag, opening a switch on the first conduit, enabling the red blood cells positioned at the bottommost layer in the centrifugation bag to flow into the red blood cell collection bag, and cutting off the first conduit in a sterile heat sealing manner when the white blood cell layer is lowered to the corresponding white blood cell membrane layer indicating line;
placing the centrifuge bag between two splints of the cell clip, opening a switch on the second conduit, operating the control device to draw the two splints together, squeezing the centrifuge bag to make the upper plasma flow upwards and enter the plasma collecting bag through the second conduit, and cutting off the second conduit by aseptic heat sealing when the leucocyte layer rises to the corresponding leucocyte membranous layer indicating line;
opening a switch on the third conduit to communicate the erythrocyte collecting bag with the plasma collecting bag, and after the internal substances are mixed, connecting the transfusion device with the needle insertion port to return the mixture to the blood-sampled person;
the first connector and the second connector are aseptically connected, the needle tube is communicated with the inside of the centrifugal bag, and the blood components remaining in the centrifugal bag, which are mainly white blood cells, are introduced into the apparatus of the next step through the needle tube.
The invention has the advantages and beneficial effects that: the invention has simple structure, can realize the extraction of active white blood cells, and the residual blood components are in a sterile environment and can be returned to the body of a blood-sampled person, thereby being capable of carrying out a large amount of white blood cells without wasting the blood of the blood-sampled person and reducing the influence on the health of the blood-sampled person as much as possible.
The centrifugal bag, the erythrocyte collecting bag, the plasma collecting bag and the connecting conduit thereof are disposable articles, the cell clamp which is not contacted with blood and the guide needle made of high-temperature resistant materials can be repeatedly used, the guide needle needs to be sterilized, and a high-temperature bacterium coming mode is preferred.
Drawings
Fig. 1 is a schematic view of the overall structure of an embodiment.
FIG. 2 is a schematic diagram showing the structure of a cell holder according to the example.
FIG. 3 is a schematic top view of an alternate position of the cell holder.
Fig. 4 is a schematic view of the installation relationship between the first interface and the guide needle in the embodiment.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "disposed" on another element, it can be directly disposed or attached to the other element or intervening elements may also be present.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
Example 1
As shown in fig. 1-4, an active white blood cell collecting device comprises a centrifuge bag 1, a red blood cell collecting bag 2, a plasma collecting bag 3, a cell clamp 4 and a diversion needle 5, wherein an inlet tube 6 connected with a blood taking needle, a first conduit 7 connected with the red blood cell collecting bag 2 and a first connector 8 used for connecting the diversion needle 5 are arranged at the upper part of the centrifuge bag 1, a second conduit 9 connected with the plasma collecting bag 3 is arranged at the bottom of the centrifuge bag 1, a third conduit 10 is arranged between the red blood cell collecting bag 2 and the plasma collecting bag 3, a needle insertion opening 20 is further arranged on the red blood cell collecting bag 2, switches 11 are arranged on the inlet tube 6, the first conduit 7, the second conduit 9 and the third conduit 10, and a white blood cell membrane layer indicating line 12 is respectively arranged at the position of the centrifuge bag 1 close to the upper end and the lower end;
the cell clamp 4 is two clamping plates 13 with one ends hinged together, and also comprises a control device 14 for controlling the two clamping plates 13 to open and close;
the guide needle 5 comprises a needle tube 15 with the length of 10-20cm and a second connector 16 which is arranged at one end of the needle tube 15 and is matched with the first connector 8.
The first connector 8 is a short pipe 17 connected with the interior of the centrifugal bag 1, an external thread 18 arranged at one end of the short pipe 17, and a first cap 19 screwed on the external thread 18 through threads to seal the short pipe 17, the second connector 16 is a second cap, the central opening of the second cap is connected with the needle tube 15, and the second cap and the needle tube 15 are of an integrated structure.
Preferably, the guide needle 5 is made of high-temperature resistant plastic or metal material, so that the guide needle can be reused after sterilization, and the cost is saved.
The bottom of the centrifugal bag 1 can be provided with a hanging hole, so that the inversion operation of the centrifugal bag is convenient.
The method of using the activated leukocyte collection device of embodiment 1, comprising the steps of:
the free end of the blood inlet pipe 6 is connected with a blood taking needle, a corresponding switch is opened, blood is drained into the centrifugal bag 1, and sterile heat sealing cutting is carried out on the blood inlet pipe 6 after the required amount of blood is received;
placing the centrifugal bag 1, the erythrocyte collecting bag 2 and the plasma collecting bag 3 into a centrifuge for centrifugal operation to separate blood into layers;
inverting the centrifuged centrifugation bag 1, opening a switch on the first conduit 7 to enable the red blood cells positioned at the bottommost layer in the centrifugation bag 1 to flow into the red blood cell collection bag 2, and cutting off the first conduit 7 in a sterile heat sealing manner when the white blood cell layer is lowered to the corresponding white blood cell indication line 12; the corresponding leucocyte membrane layer indicator line 12 here refers to the leucocyte membrane layer indicator line which is located below during operation;
placing the centrifugal bag 1 between two clamping plates 13 of the cell clamp 4 in an inverted manner, opening a switch on the second conduit 9, operating the control device 14 to enable the two clamping plates 13 to be closed, extruding the centrifugal bag 1 to enable upper-layer plasma to flow upwards and enter the plasma collecting bag 3 through the second conduit 9, and cutting off the second conduit through aseptic heat sealing when a leucocyte layer rises to a corresponding leucocyte membrane layer indicating line; the corresponding leukocyte membrane layer indicator 12 here refers to the leukocyte membrane layer indicator lying above during operation;
opening the switch on the third conduit 10 to communicate the erythrocyte collecting bag 2 with the plasma collecting bag 3, introducing the plasma into the erythrocyte collecting bag, mixing the internal substances, connecting the transfusion device with the needle insertion port to return the mixture to the blood-sampled person, and introducing the plasma into the erythrocyte collecting bag to avoid unnecessary transportation of the erythrocytes and prevent the erythrocytes from breaking;
the first adapter 8 and the second adapter 5 are aseptically connected, the needle tube 15 is communicated with the inside of the centrifugal bag 2, and the blood components remaining in the centrifugal bag 1, mainly white blood cells, are introduced into the container of the next step through the needle tube 15. The length of the needle tube is required to be 10-20cm, a plurality of containers are respectively filled at one time, otherwise, a dropper is needed to repeatedly transfer blood components for a plurality of times, and the risk of pollution is caused.
The invention has the advantages and beneficial effects that: the invention has simple structure, can realize the extraction of active white blood cells, and the residual blood components are in a sterile environment and can be returned to the body of a blood-sampled person, thereby being capable of carrying out a large amount of white blood cells without wasting the blood of the blood-sampled person and reducing the influence on the health of the blood-sampled person as much as possible.
The working principle of the invention is as follows: the invention utilizes the principle of layering after blood centrifugation to respectively guide out and mix the blood plasma and the red blood cells from the upper part and the lower part and then return the mixture to the blood-sampled person, thereby ensuring enough white blood cells, reducing the blood loss of the blood-sampled person to the maximum extent and reducing the adverse effect on the body to the minimum. Meanwhile, the device has simple structure, part of components can be used repeatedly, and the cost is low. According to the common medical knowledge, in order to ensure the quality of the blood material, an appropriate amount of anticoagulant is previously filled in a place where it is needed, such as a centrifugal bag, to avoid blood cell coagulation.
The embodiments of the present invention are described only for the preferred embodiments of the present invention, and not for the limitation of the concept and scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the design concept of the present invention shall fall into the protection scope of the present invention, and the technical content of the present invention which is claimed is fully set forth in the claims.
Claims (4)
1. An activated leukocyte collection device, comprising: the blood taking and guiding device comprises a centrifugal bag, an erythrocyte collecting bag, a plasma collecting bag, a cell clamp and a guiding needle, wherein the centrifugal bag is provided with a blood inlet pipe connected with a blood taking needle, a first conduit connected with the erythrocyte collecting bag at the upper part of the centrifugal bag and a first connector used for connecting the guiding needle, a second conduit connected with the plasma collecting bag is arranged at the bottom of the centrifugal bag, a third conduit is arranged between the erythrocyte collecting bag and the plasma collecting bag, a needle inserting port is also arranged on the erythrocyte collecting bag, switches are arranged on the blood inlet pipe, the first conduit, the second conduit and the third conduit, and a leukocyte membrane layer indicating line is respectively arranged at the position, close to the upper end and the lower end, of the centrifugal bag;
the cell clamp is two clamping plates with one ends hinged together, and the cell clamp also comprises a control device for controlling the two clamping plates to open and close;
the guide needle comprises a needle tube with the length of 10-20cm and a second joint which is arranged at one end of the needle tube and is matched with the first joint.
2. The activated leukocyte collection device of claim 1 wherein: the first connector is a short pipe connected with the interior of the centrifugal bag, an external thread arranged at one end of the short pipe and a first cap screwed on the external thread through threads to seal the short pipe, the second connector is a second cap, and the central opening of the second cap is connected with the needle tube or the second cap and the needle tube are of an integrated structure.
3. The activated leukocyte collection device of claim 1 wherein: the guide needle is made of high-temperature resistant plastic or metal material.
4. The activated leukocyte collection device of claim 1 wherein: the bottom of the centrifugal bag is provided with a hanging hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921194781.7U CN210620844U (en) | 2019-07-27 | 2019-07-27 | Active white blood cell collecting device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921194781.7U CN210620844U (en) | 2019-07-27 | 2019-07-27 | Active white blood cell collecting device |
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| CN210620844U true CN210620844U (en) | 2020-05-26 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110484502A (en) * | 2019-07-27 | 2019-11-22 | 王太林 | A kind of activity leukocyte collection device and collection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110484502A (en) * | 2019-07-27 | 2019-11-22 | 王太林 | A kind of activity leukocyte collection device and collection method |
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