CN110484502A - A kind of activity leukocyte collection device and collection method - Google Patents
A kind of activity leukocyte collection device and collection method Download PDFInfo
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- CN110484502A CN110484502A CN201910685325.0A CN201910685325A CN110484502A CN 110484502 A CN110484502 A CN 110484502A CN 201910685325 A CN201910685325 A CN 201910685325A CN 110484502 A CN110484502 A CN 110484502A
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- 210000000265 leukocyte Anatomy 0.000 title claims abstract description 16
- 230000000694 effects Effects 0.000 title claims description 11
- 238000000034 method Methods 0.000 title claims description 8
- 238000005119 centrifugation Methods 0.000 claims abstract description 41
- 210000004369 blood Anatomy 0.000 claims abstract description 40
- 239000008280 blood Substances 0.000 claims abstract description 40
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 33
- 210000002381 plasma Anatomy 0.000 claims abstract description 30
- 210000004027 cell Anatomy 0.000 claims abstract description 17
- 210000004204 blood vessel Anatomy 0.000 claims abstract description 14
- 239000006071 cream Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- 230000008676 import Effects 0.000 claims description 3
- 238000001802 infusion Methods 0.000 claims description 3
- 239000007769 metal material Substances 0.000 claims description 3
- 230000000630 rising effect Effects 0.000 claims description 2
- 230000008821 health effect Effects 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 4
- 210000005259 peripheral blood Anatomy 0.000 description 4
- 239000011886 peripheral blood Substances 0.000 description 4
- 230000036541 health Effects 0.000 description 3
- 239000012503 blood component Substances 0.000 description 2
- 238000010241 blood sampling Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000005934 immune activation Effects 0.000 description 1
- 230000006058 immune tolerance Effects 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002906 medical waste Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/02—Blood transfusion apparatus
- A61M1/029—Separating blood components present in distinct layers in a container, not otherwise provided for
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
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- Engineering & Computer Science (AREA)
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- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Veterinary Medicine (AREA)
- Vascular Medicine (AREA)
- Anesthesiology (AREA)
- Public Health (AREA)
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Abstract
The present invention relates to a kind of active leukocyte collection devices, it is characterized by comprising centrifugation bags, red blood cell bag, blood plasma collecting bag, cell folder and stream guidance needle, the top of the centrifugation bag is provided with connection blood taking needle and enters blood vessel, connect the first conduit of red blood cell bag, and the first connector for connecting stream guidance needle, the bottom of the centrifugation bag is provided with the second conduit of connection blood plasma collecting bag, third conduit is provided between red blood cell bag and blood plasma collecting bag, it is described enter blood vessel, first conduit, second conduit, switch is provided on third conduit, a leucocyte index line is respectively arranged in the centrifugation bag at top and bottom.The configuration of the present invention is simple, it is at low cost, it is again small to the health effect of person of blood collecting may largely to collect active leucocyte.
Description
Technical field
The present invention relates to the leukocyte collection devices in blood, the in particular to separated structure of leucocyte.
Background technique
Cellular immunotherapy is widely used in oncotherapy, is that acquisition human autoimmune's cell is (mainly white thin
Born of the same parents), by vitro culture, increase its quantity thousandfold, then the enhancing of targeting killing ability feeds back to human body again to kill
Blood and tissue in pathogen, cancer cell, mutation cell, break immune tolerance, activation and enhancing body immunocompetence,
Take into account the double effects for the treatment of and health care.
In oncotherapy, conventional single acquisition 50-100ml(syringe) peripheral blood carries out external aim cell culture,
But effect is undesirable, and initial cell obtains inadequate in single acquisition.The quantity of leucocyte for being primarily due to patient is less, and acquires
Red blood cell and blood plasma afterwards can not be fed back to patient, caused a part of haemocyte to be lost, therefore can not largely take a blood sample, otherwise can
Patient harm.In addition, active leucocyte applies also for the exploitation of leucocyte class diagnostic reagent.
Existing leucocyte filtration device can separate leucocyte from blood, but the leucocyte after separation loses work
Property, generally with device as treatment of!medical waste.If the method for keeping leukocyte activity can be found, to the synthesis of blood resource
Using will be a new outlet.
Peripheral blood can be layered after centrifugal treating, from top to bottom respectively plasma layer, leucocyte and platelet layer and red thin
Born of the same parents' layer.
Summary of the invention
The purpose of the present invention is being improved and being innovated for disadvantage present in background technique and problem, a kind of energy is provided
Largely collect the simple mechanism that active leucocyte and residual blood can be fed back.
The technical scheme is that construct a kind of active leukocyte collection device, including centrifugation bag, red blood cell bag,
Blood plasma collecting bag, cell folder and stream guidance needle, the top for entering blood vessel, being centrifuged bag that the centrifugation bag is provided with connection blood taking needle are set
It is equipped with the first conduit of connection red blood cell bag, and the first connector for connecting stream guidance needle, the bottom of the centrifugation bag
It is provided with the second conduit of connection blood plasma collecting bag, third conduit, institute are provided between red blood cell bag and blood plasma collecting bag
State and be additionally provided with contact pin mouth on red blood cell bag, it is described enter blood vessel, the first conduit, the second conduit, be respectively provided on third conduit
There is switch, a leucocyte film layer index line is respectively arranged in the centrifugation bag at top and bottom；
The cell folder is the hinged clamping plate in two pieces of one end, further includes the control device for controlling the folding of two pieces of clamping plates；
The stream guidance needle includes the needle tubing and be arranged in needle tubing one end that length is 10-20cm, is matched with the first connector
Second connector.
First connector is to connect the short tube being centrifuged inside bag, the external screw thread of short tube one end is arranged in, and passing through
Screw thread is tightened on the first nut cap that short tube is closed on external screw thread, and second connector is the second nut cap, second lid
Cap central opening connection needle tubing or the second nut cap are structure as a whole with needle tubing.
The stream guidance needle is made of high-temperature resistance plastice or metal material.
More preferably, the centrifugation bag bottom is provided with hanging hole.
Another technical solution of the invention is the method for carrying out leukocyte collection using foregoing active leukocyte collection device,
Include:
The free end connection blood taking needle for entering blood vessel, opens corresponding switch, by blood drainage to centrifugation bag, is collected into aequum
Blood after, carry out sterile heat seal truncation to blood vessel is entered；
Centrifugation bag, red blood cell bag, blood plasma collecting bag are put into centrifuge and carry out centrifugally operated, is layered blood；
Centrifugation bag after centrifugation is inverted, the first supravasal switch is opened, makes to be centrifuged the red blood cell stream for being located at the bottom in bag
Enter red blood cell bag, when leukocytic cream is down at corresponding leucocyte film layer index line, the sterile heat seal of the first conduit is cut
It is disconnected；
Bag will be centrifuged to be inverted between two pieces of clamping plates of cell folder, open the second supravasal switch, operating control device makes
Two pieces of clamping plates are drawn close, and centrifugation bag is squeezed, and are kept upper plasma upper and are entered blood plasma collecting bag by the second conduit, work as leukocytic cream
When rising at corresponding leucocyte film layer index line, the sterile heat seal of the second conduit is truncated；
The supravasal switch of third is opened, red blood cell bag and blood plasma collecting bag are communicated, after inner material mixing, infusion
Device connecting contact pin mouth feeds back mixture to person of blood collecting；
By the first connector and the sterile connection of the second connector, make to communicate inside needle tubing and centrifugation bag, by remaining blood in centrifugation bag
Composition imports the instrument of the next step by needle tubing, which is mainly leucocyte.
The advantages of the present invention: the configuration of the present invention is simple, it can be achieved that active leucocyte extraction, and it is remaining
Blood constituent is in gnotobasis, can be fed back in person of blood collecting's body, therefore can carry out a large amount of leucocytes without wasting quilt
The blood of blood sampling person reduces the influence to its health to the greatest extent, can the progress of one acquisition 100-400ml peripheral blood using the present invention
Leucocyte extracts.
It is disposable product that the present invention, which is centrifuged bag, red blood cell bag, blood plasma collecting bag and its connecting conduit, without contacting
It can be used repeatedly for the cell folder of blood and the stream guidance needle made of high temperature resistant material, and stream guidance needle needs to carry out sterilization treatment, first
High temperature is selected to carry out bacterium mode.
Detailed description of the invention
Fig. 1 is the overall structure diagram of one embodiment.
Fig. 2 is the structural schematic diagram that cell presss from both sides in embodiment.
Fig. 3 is the vertical view alternate position schematic diagram of cell folder.
Fig. 4 is the installation relation schematic diagram of first interface and stream guidance needle in embodiment.
Specific embodiment
To facilitate the understanding of the present invention, a more comprehensive description of the invention is given in the following sections with reference to the relevant attached drawings.In attached drawing
Give preferred embodiment of the invention.But the invention can be realized in many different forms, however it is not limited to this paper institute
The embodiment of description.On the contrary, purpose of providing these embodiments is make it is more thorough and comprehensive to the disclosure.
It should be noted that when element be considered as " setting " on the other element, it can be directly setting or company
Connect on the other element or may be simultaneously present centering elements.
Unless otherwise defined, the skill of all technical and scientific terms and technical field of the invention used herein
The normally understood meaning of art personnel is identical.Term used in the description is intended merely to describe specifically to implement purpose, is not
It is intended to limit the present invention.
Embodiment 1
As shown in Figs 1-4, a kind of active leukocyte collection device, including centrifugation bag 1, red blood cell bag 2, blood plasma collecting bag 3,
Cell folder 4 and stream guidance needle 5, the top of the centrifugation bag 1 are provided with entering blood vessel 6, connecting red blood cell for connection blood taking needle
The bottom of first conduit 7 of bag 2, and the first connector 8 for connecting stream guidance needle 5, the centrifugation bag 1 is provided with connection blood plasma
Second conduit 9 of collecting bag 3, is provided with third conduit 10, the red blood cell between red blood cell bag 2 and blood plasma collecting bag 3
Be additionally provided with contact pin mouth 20 on collecting bag 2, it is described enter blood vessel 6, the first conduit 7, the second conduit 9, be respectively provided on third conduit 10
There is switch 11, a leucocyte film layer index line 12 is respectively arranged in the centrifugation bag 1 at top and bottom；
The cell folder 4 clamping plate 13 hinged for two pieces of one end further includes the control dress for controlling two pieces of clamping plates 13 and opening and closing
Set 14；
The stream guidance needle 5 includes the needle tubing 15 and be arranged in 15 one end of needle tubing, with 8 phase of the first connector that length is 10-20cm
Second connector 16 of cooperation.
First connector 8 is to connect the short tube 17 being centrifuged inside bag 1, the external screw thread 18 that 17 one end of short tube is arranged in,
And the first nut cap 19 that short tube 17 is closed on external screw thread 18 is tightened on by screw thread, second connector 16 is the second lid
Cap, the second nut cap central opening connect needle tubing 15, and the second nut cap is structure as a whole with needle tubing 15.
More preferably, the stream guidance needle 5 is made of high-temperature resistance plastice or metal material, to reuse after sterilizing, saves
About cost.
Hanging hole can be set in 1 bottom of centrifugation bag, facilitates the inversion operation of centrifugation bag.
Embodiment 2
The application method of activity leukocyte collection device described in embodiment 1, comprising the following steps:
The free end connection blood taking needle for entering blood vessel 6, opens corresponding switch, by blood drainage to centrifugation bag 1, receives needed for wanting
After the blood of amount, sterile heat seal truncation is carried out to blood vessel 6 is entered；
Centrifugation bag 1, red blood cell bag 2, blood plasma collecting bag 3 are put into centrifuge and carry out centrifugally operated, is layered blood；
Centrifugation bag 1 after centrifugation is inverted, the switch on the first conduit 7 is opened, makes to be centrifuged the red blood cell for being located at the bottom in bag 1
Red blood cell bag 2 is flowed into, when leukocytic cream is down at corresponding leucocyte index line 12, by the sterile heat seal of the first conduit 7
Truncation；Here corresponding leucocyte film layer index line 12 refers to underlying leucocyte film layer index line when operation；
Bag 1 will be centrifuged to be inverted between two pieces of clamping plates 13 of cell folder 4, open the switch on the second conduit 9, operation control dress
Setting 14 draws close two pieces of clamping plates 13, squeezes centrifugation bag 1, keeps upper plasma upper and enters blood plasma collecting bag by the second conduit 9
3, when leukocytic cream rises at corresponding leucocyte film layer index line, the sterile heat seal of the second conduit is truncated；Here corresponding
Leucocyte film layer index line 12 refers to the leucocyte film layer index line being located above when operation；
The switch on third conduit 10 is opened, red blood cell bag 2 and blood plasma collecting bag 3 is communicated, blood plasma is imported into red blood cell
Mixture is fed back infusion device connecting contact pin mouth to person of blood collecting after inner material mixing, blood plasma is imported red by collecting bag
Cell collecting bag can prevent red blood cell rupture to avoid the meaningless conveying of red blood cell；
By the first connector 8 and the sterile connection of the second connector 5, make to communicate inside needle tubing 15 and centrifugation bag 2, it will be remaining in centrifugation bag 1
Blood component by needle tubing 15 import the next step container, which is mainly leucocyte.Needle tubing length requirement
For 10-20cm, multiple containers are disposably perfused respectively, otherwise needs to use dropper and repeatedly shifts blood component repeatedly,
There is contaminated risk.
The advantages of the present invention: the configuration of the present invention is simple, it can be achieved that active leucocyte extraction, and it is remaining
Blood constituent is in gnotobasis, can be fed back in person of blood collecting's body, therefore can carry out a large amount of leucocytes without wasting quilt
The blood of blood sampling person reduces the influence to its health to the greatest extent, can the progress of one acquisition 100-400ml peripheral blood using the present invention
Leucocyte extracts.
The working principle of the invention is: the present invention utilizes the principle being layered after centrifugal blood, from above and below will respectively
Blood plasma and red blood cell are fed back after exporting and mixing to person of blood collecting, can guarantee enough leucocytes, both that can reduce to greatest extent
The blood loss of person of blood collecting is preferably minimized the adverse effect to body.Meanwhile the structure of apparatus of the present invention is simple, part
Component energy Reusability, it is low in cost.According to medicine common sense, in order to guarantee the quality of blood material, in the place of needs, such as from
In heart bag, it is packed into appropriate anti-coagulants in advance to avoid haemocyte solidification.
Embodiment of the present invention is only the description carried out to the preferred embodiment of the present invention, not to the present invention
Conception and scope is defined, and under the premise of not departing from design philosophy of the present invention, engineers and technicians are to this hair in this field
The all variations and modifications that bright technical solution is made should all fall into protection scope of the present invention, the claimed skill of the present invention
Art content, is all described in the claims.
Claims (5)
1. a kind of activity leukocyte collection device, it is characterised in that: including centrifugation bag, red blood cell bag, blood plasma collecting bag, thin
Born of the same parents' folder and stream guidance needle, the centrifugation bag are provided with the top connection red blood cell for entering blood vessel, being centrifuged bag of connection blood taking needle
The bottom of first conduit of bag, and the first connector for connecting stream guidance needle, the centrifugation bag is provided with connection plasma collection
Second conduit of bag is provided with third conduit, goes back on the red blood cell bag between red blood cell bag and blood plasma collecting bag
Be provided with contact pin mouth, it is described enter blood vessel, the first conduit, the second conduit, be provided with switch on third conduit, the centrifugation bag leans on
A leucocyte film layer index line is respectively set at nearly top and bottom；
The cell folder is the hinged clamping plate in two pieces of one end, further includes the control device for controlling the folding of two pieces of clamping plates；
The stream guidance needle includes the needle tubing and be arranged in needle tubing one end that length is 10-20cm, is matched with the first connector
Second connector.
2. activity leukocyte collection device according to claim 1, it is characterised in that: first connector be connection from
Short tube inside heart bag is arranged in the external screw thread of short tube one end, and is tightened on external screw thread by screw thread to close short tube
First nut cap, second connector are the second nut cap, the second nut cap central opening connection needle tubing or the second nut cap
It is structure as a whole with needle tubing.
3. activity leukocyte collection device according to claim 1, it is characterised in that: the stream guidance needle is moulded by high temperature resistant
Material or metal material are made.
4. activity leukocyte collection device according to claim 1, it is characterised in that: the centrifugation bag bottom is provided with
Hanging hole.
5. the method for carrying out leukocyte collection using active leukocyte collection device described in claim 1, which is characterized in that packet
It includes:
The free end connection blood taking needle for entering blood vessel, opens corresponding switch, by blood drainage to centrifugation bag, is collected into aequum
Blood after, carry out sterile heat seal truncation to blood vessel is entered；
Centrifugation bag, red blood cell bag, blood plasma collecting bag are put into centrifuge and carry out centrifugally operated, is layered blood；
Centrifugation bag after centrifugation is inverted, the first supravasal switch is opened, makes to be centrifuged the red blood cell stream for being located at the bottom in bag
Enter red blood cell bag, when leukocytic cream is down at corresponding leucocyte film layer index line, the sterile heat seal of the first conduit is cut
It is disconnected；
Bag will be centrifuged to be inverted between two pieces of clamping plates of cell folder, open the second supravasal switch, operating control device makes
Two pieces of clamping plates are drawn close, and centrifugation bag is squeezed, and are kept upper plasma upper and are entered blood plasma collecting bag by the second conduit, work as leukocytic cream
When rising at corresponding leucocyte film layer index line, the sterile heat seal of the second conduit is truncated；
The supravasal switch of third is opened, red blood cell bag and blood plasma collecting bag are communicated, after inner material mixing, infusion
Device connecting contact pin mouth feeds back mixture to person of blood collecting；
By the first connector and the sterile connection of the second connector, make to communicate inside needle tubing and centrifugation bag, by remaining blood in centrifugation bag
Composition imports the instrument of the next step by needle tubing, which is mainly leucocyte.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910685325.0A CN110484502A (en) | 2019-07-27 | 2019-07-27 | A kind of activity leukocyte collection device and collection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910685325.0A CN110484502A (en) | 2019-07-27 | 2019-07-27 | A kind of activity leukocyte collection device and collection method |
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| CN110484502A true CN110484502A (en) | 2019-11-22 |
Family
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910685325.0A Pending CN110484502A (en) | 2019-07-27 | 2019-07-27 | A kind of activity leukocyte collection device and collection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111548992A (en) * | 2020-05-28 | 2020-08-18 | 章毅 | Method and device for preparing immune cells in blood in large scale |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5102407A (en) * | 1990-03-13 | 1992-04-07 | Miles Inc. | Blood separation system |
| CN2194721Y (en) * | 1994-06-21 | 1995-04-19 | 徐州市红十字中心血站 | Link bag for separation of blood white membrane |
| US5523004A (en) * | 1992-12-04 | 1996-06-04 | Terumo Kabushiki Kaisha | Method for treatment of blood using a blood bag |
| CN201205379Y (en) * | 2008-05-26 | 2009-03-11 | 四川南格尔生物医学股份有限公司 | Disposable closed single blood-sampling blood cell separator |
| CN103028154A (en) * | 2011-12-14 | 2013-04-10 | 南京双威生物医学科技有限公司 | Platelet separation bag and method for manually separating platelet |
| CN104684596A (en) * | 2012-07-25 | 2015-06-03 | 门布拉内有限公司 | Mobile system for separating donor blood by means of gravitational force |
| CN204910103U (en) * | 2015-07-03 | 2015-12-30 | 四川南格尔生物科技有限公司 | Top end bi -pass blood bag structure |
| CN210620844U (en) * | 2019-07-27 | 2020-05-26 | 王太林 | Active white blood cell collecting device |
-
2019
- 2019-07-27 CN CN201910685325.0A patent/CN110484502A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5102407A (en) * | 1990-03-13 | 1992-04-07 | Miles Inc. | Blood separation system |
| US5523004A (en) * | 1992-12-04 | 1996-06-04 | Terumo Kabushiki Kaisha | Method for treatment of blood using a blood bag |
| CN2194721Y (en) * | 1994-06-21 | 1995-04-19 | 徐州市红十字中心血站 | Link bag for separation of blood white membrane |
| CN201205379Y (en) * | 2008-05-26 | 2009-03-11 | 四川南格尔生物医学股份有限公司 | Disposable closed single blood-sampling blood cell separator |
| CN103028154A (en) * | 2011-12-14 | 2013-04-10 | 南京双威生物医学科技有限公司 | Platelet separation bag and method for manually separating platelet |
| CN104684596A (en) * | 2012-07-25 | 2015-06-03 | 门布拉内有限公司 | Mobile system for separating donor blood by means of gravitational force |
| US20150209494A1 (en) * | 2012-07-25 | 2015-07-30 | Membrana Gmbh | Mobile system for separating donor blood by means of gravitational force |
| CN204910103U (en) * | 2015-07-03 | 2015-12-30 | 四川南格尔生物科技有限公司 | Top end bi -pass blood bag structure |
| CN210620844U (en) * | 2019-07-27 | 2020-05-26 | 王太林 | Active white blood cell collecting device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111548992A (en) * | 2020-05-28 | 2020-08-18 | 章毅 | Method and device for preparing immune cells in blood in large scale |
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Application publication date: 20191122 |