CN205907261U - Circulation tumor cells autosegregation device - Google Patents
Circulation tumor cells autosegregation device Download PDFInfo
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- CN205907261U CN205907261U CN201620821384.8U CN201620821384U CN205907261U CN 205907261 U CN205907261 U CN 205907261U CN 201620821384 U CN201620821384 U CN 201620821384U CN 205907261 U CN205907261 U CN 205907261U
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- cell
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- circulating tumor
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Abstract
The utility model relates to a circulation tumor cells autosegregation device, the red blood cell analyses and explains adds the red blood cell in the unit and analyses and explains liquid and blood, and the red blood cell analyses and explains the red blood cell of liquid in with blood and analyses and explains, circulation tumor cells separating element includes circulation pipe way, cell separating module, circulation control module, circulation control module install on the circulation pipe way, the cell separating module install on the circulation pipe way, circulation control module control blood is in circulation pipe says that the mesocycle flows, cell separating module will the white cell filtration of blood during circulation pipe says, circulation tumor cells collector unit will circulation pipe says that the circulation tumor cells who flows collects. The utility model relates to a circulation tumor cells autosegregation device has implemented the process to circulation tumor cells's integrated autosegregation, has improved circulation tumor cells's separation efficiency to the intact of cell can be kept well at disengaging process, the analysis is conveniently carried out.
Description
Technical field
The utility model is related to a kind of automatic cell separating device, and more particularly, to a kind of circulating tumor cell is automatically separated dress
Put.
Background technology
With the development of microflow control technique, research field increases, and is always with micro-fluidic screening circulating tumor cell and studies
Focus.Three steps typically to be carried out to circulating tumor cell, the first step is to remove red blood cell；Second step is to filter out circulation
Tumour cell；3rd step is whether the cell that evaluation and screening goes out can confirm that as cancer cell, the enforcement to this three steps at present,
Automaticity is relatively low, and needs to separate to complete these three steps, lacks the system that automatic integratedization completes these steps
Device, makes the automatic degree of separation process not high, inefficiency.Further, since implementing step by step, blood and cell may be made in reality
It is damaged during applying.
Utility model content
The technical problem that the utility model solves is: builds a kind of circulating tumor cell automatic separating apparatus, overcomes existing
In technology circulating tumor cell separation process, automatic degree is not high, the technical problem of inefficiency.
The technical solution of the utility model is: builds a kind of circulating tumor cell automatic separating apparatus, breaks including red blood cell
Solution unit, circulating tumor cell separative element, circulating tumor cell collector unit, described red blood cell cracks unit, described circulation
Tumour cell separative element and described circulating tumor cell collector unit are sequentially connected, described red blood cell crack add in unit red
Cell cracks liquid and blood, and red blood cell cracks liquid and cracks the red blood cell in blood, described circulating tumor cell separative element bag
Include circulating line, cell separation module, circulation control module, described circulation control module is arranged on described circulating line, institute
State cell separation module to be arranged on described circulating line, described circulation control module controls blood to follow in described circulating line
Circulation moves, and the leukoreduction filter of blood in described circulating line, described circulating tumor cell are collected by described cell separation module
The circulating tumor cell that described circulating line flows out is collected by unit.
Further technical scheme of the present utility model is: described cell separation module includes leukoreduction filter net.
Further technical scheme of the present utility model is: described circulating line is circulating line.
Further technical scheme of the present utility model is: described red blood cell cracks unit and separates with described circulating tumor cell
First shutoff valve is set between unit.
Further technical scheme of the present utility model is: described circulating tumor cell separative element and described circulating tumor are thin
Second shutoff valve is set between born of the same parents' collector unit.
Further technical scheme of the present utility model is: also includes reflux line, described cell separation module includes also wrapping
Include the container collecting tissue fluid and tissue fluid screen pack, described container passes through described tissue fluid screen pack and described reflux line
Connect described circulating line.
Further technical scheme of the present utility model is: described circulation control module is blood pump.
Further technical scheme of the present utility model is: described circulating tumor cell collector unit includes negative pressure cell and collects
Module.
Further technical scheme of the present utility model is: described circulation control module connect described red blood cell crack unit and
Described circulating line.
Further technical scheme of the present utility model is: also includes tumour cell marking fluid and adds mouth.
Of the present utility model having the technical effect that builds a kind of circulating tumor cell automatic separating apparatus, breaks including red blood cell
Solution unit, circulating tumor cell separative element, circulating tumor cell collector unit, described red blood cell cracks unit, described circulation
Tumour cell separative element and described circulating tumor cell collector unit are sequentially connected, described red blood cell crack add in unit red
Cell cracks liquid and blood, and red blood cell cracks liquid and cracks the red blood cell in blood, described circulating tumor cell separative element bag
Include circulating line, cell separation module, circulation control module, described circulation control module is arranged on described circulating line, institute
State cell separation module to be arranged on described circulating line, described circulation control module controls blood to follow in described circulating line
Circulation moves, and the leukoreduction filter of blood in described circulating line, described circulating tumor cell are collected by described cell separation module
The circulating tumor cell that described circulating line flows out is collected by unit.A kind of circulating tumor cell of the utility model is automatically separated dress
Put, implement the process that the integration to circulating tumor cell is automatically separated, improve the separative efficiency of circulating tumor cell, and
And the intact of cell can be kept well in separation process, conveniently it is analyzed.
Brief description
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the structural representation of the utility model circulating tumor cell separative element.
Specific embodiment
With reference to specific embodiment, technical solutions of the utility model are further illustrated.
As shown in figure 1, specific embodiment of the present utility model is: build a kind of circulating tumor cell and be automatically separated dress
Put, crack unit a, circulating tumor cell separative element b, circulating tumor cell collector unit c, described red blood cell including red blood cell
Crack unit a, described circulating tumor cell separative element b and described circulating tumor cell collector unit c to be sequentially connected, described red
Cell cracks and adds red blood cell to crack liquid and blood in unit a, and red blood cell cracks liquid and cracks the red blood cell in blood, described follows
Ring tumour cell separative element b includes circulating line 5, cell separation module 6, circulation control module 4, described circulation control module
4 are arranged on described circulating line 5, and described cell separation module 6 is arranged on described circulating line 5, described ring flow control module
Block 4 controls blood to circulate in described circulating line 5, and described cell separation module 6 is by blood in described circulating line 5
Leukoreduction filter, the circulating tumor cell that described circulating line 5 flows out is collected by described circulating tumor cell collector unit c.Tool
In body embodiment, described circulation control module 4 is blood pump, implements to circulate by blood pump, and passes through described circulation
Control module 4 can realize flow control, so more easily controls the separation process of circulating tumor cell.
As shown in Figure 1 and Figure 2, specific implementation process of the present utility model is: described red blood cell cracks unit and includes blood appearance
Device 1, described red blood cell cracks and adds red blood cell to crack liquid and blood in the blood vessel 1 of unit a, and red blood cell cracks liquid by blood
In red blood cell crack, red blood cell crack after blood enter described circulating tumor cell separative element b in, ring flow control module
Block 4 controls blood to circulate in described circulating line 5, when blood flow is to described cell separation module 6, described
Cell separation module 6 arranges leukoreduction filter net 17, and the leukoreduction filter net 17 of described cell separation module 6 is by described circulating pipe
The leukoreduction filter of blood in road 5, the tissue fluid after filtration is flowed in container 18.Because circulating tumor cell volume is larger,
Therefore, circulating tumor cell still circulates in described circulating line 5, so that leukoreduction filter is cleaner.Work as filtration
After the completion of, described circulating line 5 is opened with described circulating tumor cell collector unit c junction, and described circulating tumor cell is received
Collection unit c collects the circulating tumor cell that described circulating line 5 flows out.
As shown in figure 1, preferred embodiment of the present utility model is: described circulating line 5 is circulating line, and blood is in ring
Flow tube circulates in road, and circulating line is convenient to be circulated, and can be easily separated.
As shown in figure 1, preferred embodiment of the present utility model is: described red blood cell cracks unit s and described circulation is swollen
First shutoff valve 3 is set between oncocyte separative element b.In blood entry port, red blood cell is added to crack liquid and blood, first section
Stream valve 3 cuts out, and red blood cell cracks liquid and cracks the red blood cell in blood, and after the completion of cracking, the first shutoff valve 3 is opened, red blood cell
Blood after cracking flows into described circulating tumor cell separative element b.
As shown in figure 1, preferred embodiment of the present utility model is: described circulating line 5 also sets up the 3rd shutoff valve 9,
Between described circulating tumor cell separative element b and described circulating tumor cell collector unit c, the second shutoff valve 10 is set.Institute
When stating the blood flow in circulating line 5, the 3rd shutoff valve 9 is opened, and the second shutoff valve 10 cuts out, and realizes described circulating line 5
In blood circulation flowing.During blood flow in described circulating line 5, the second shutoff valve 10 is opened, and the 3rd shutoff valve 9 closes
Close, in described circulating line 5, detached circulating tumor cell flows in described circulating tumor cell collector unit c.
As shown in Fig. 2 preferred embodiment of the present utility model is: also include reflux line 7, described cell separation module
6 include container 18, tissue fluid screen pack 19 and the filter chamber 16 also including collecting tissue fluid, and tissue fluid screen pack 19 is arranged on
In filter chamber 16, filter chamber 16 is socketed on described circulating line 5, and described container 18 passes through described tissue fluid screen pack 19 and institute
State reflux line 7 and connect described circulating line 5.Tissue fluid after filtering is passed through by tissue fluid screen pack 19 by reflux line 7
It is back to described circulating line 5, proceeds to circulate, during circulating, leucocyte is more thoroughly crossed and is filtered dry
Only, leave circulating tumor cell, realize the separation of circulating tumor cell.
As shown in figure 1, preferred embodiment of the present utility model is: described circulating tumor cell collector unit c includes bearing
Pressure cell collection module 15, described circulating tumor cell collector unit c also includes tumour cell marking fluid and adds mouth 11 and thin
Born of the same parents' counting module 13, adds mouth 11 to add tumour cell marking fluid from tumour cell marking fluid, makes described circulating line 5 flow through
Circulating tumor cell be labeled.Negative pressure cell collection module 15 passes through negative pressure, so that circulating tumor cell is collected to negative pressure cell
Move in module 15, counted by cell count module 13.
As shown in figure 1, preferred embodiment of the present utility model is: described circulation control module 4 connects described red blood cell
Crack unit a and described circulating line 5.Described circulation control module 4 directly receive described red blood cell crack unit a process after
Blood, then flows into described circulating line 5 by circulation control module 4 again, is more convenient to control.
Of the present utility model having the technical effect that builds a kind of circulating tumor cell automatic separating apparatus, breaks including red blood cell
Solution unit a, circulating tumor cell separative element b, circulating tumor cell collector unit c, described red blood cell cracks unit a, described
Circulating tumor cell separative element b and described circulating tumor cell collector unit c is sequentially connected, and described red blood cell cracks unit a
Middle addition red blood cell cracks liquid and blood, and red blood cell cracks liquid and cracks the red blood cell in blood, and described circulating tumor cell divides
Include circulating line 5, cell separation module 6, circulation control module 4 from unit b, described circulation control module 4 is arranged on described
On circulating line 5, described cell separation module 6 is arranged on described circulating line 5, and described circulation control module 4 controls blood
Described circulating line 5 circulates, described cell separation module 6 by the leukoreduction filter of blood in described circulating line 5,
The circulating tumor cell that described circulating line 5 flows out is collected by described circulating tumor cell collector unit c.The utility model is a kind of
Circulating tumor cell automatic separating apparatus, implement the process that the integration to circulating tumor cell is automatically separated, improve and follow
The separative efficiency of ring tumour cell, and the intact of cell can be kept well in separation process, conveniently it is analyzed.
Above content be with reference to specific preferred embodiment further detailed description of the utility model it is impossible to
Identification is of the present utility model to be embodied as being confined to these explanations.Ordinary skill for the utility model art
For personnel, without departing from the concept of the premise utility, some simple deduction or replace can also be made, all should regard
For belonging to protection domain of the present utility model.
Claims (10)
1. a kind of circulating tumor cell automatic separating apparatus crack unit, circulating tumor cell it is characterised in that including red blood cell
Separative element, circulating tumor cell collector unit, described red blood cell cracks unit, described circulating tumor cell separative element and institute
State circulating tumor cell collector unit to be sequentially connected, described red blood cell cracks and adds red blood cell to crack liquid and blood in unit, red
Cell cracks liquid and cracks the red blood cell in blood, and described circulating tumor cell separative element includes circulating line, cell separation
Module, circulation control module, described circulation control module is arranged on described circulating line, and described cell separation module is arranged on
On described circulating line, described circulation control module controls blood to circulate in described circulating line, described cell separation
By the leukoreduction filter of blood in described circulating line, described circulating tumor cell collector unit is by described circulating line stream for module
The circulating tumor cell going out is collected.
2. according to claim 1 circulating tumor cell automatic separating apparatus it is characterised in that described cell separation module bag
Include leukoreduction filter net.
3. according to claim 1 circulating tumor cell automatic separating apparatus it is characterised in that described circulating line be annular
Pipeline.
4. according to claim 1 circulating tumor cell automatic separating apparatus it is characterised in that described red blood cell cracks unit
First shutoff valve is set and described circulating tumor cell separative element between.
5. according to claim 1 circulating tumor cell automatic separating apparatus it is characterised in that described circulating tumor cell divides
Between unit and described circulating tumor cell collector unit, the second shutoff valve is set.
6. according to claim 2 circulating tumor cell automatic separating apparatus it is characterised in that also including reflux line, institute
State container and the tissue fluid screen pack that cell separation module includes also including collecting tissue fluid, described container passes through described tissue
Liquid screen pack and described reflux line connect described circulating line.
7. according to claim 1 circulating tumor cell automatic separating apparatus it is characterised in that described circulation control module is
Blood pump.
8. according to claim 1 circulating tumor cell automatic separating apparatus it is characterised in that described circulating tumor cell receive
Collection unit includes negative pressure cell collection module.
9. according to claim 1 circulating tumor cell automatic separating apparatus it is characterised in that described circulation control module even
Connect described red blood cell and crack unit and described circulating line.
10. according to claim 1 circulating tumor cell automatic separating apparatus it is characterised in that also including tumour cell mark
Note liquid adds mouth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620821384.8U CN205907261U (en) | 2016-07-29 | 2016-07-29 | Circulation tumor cells autosegregation device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620821384.8U CN205907261U (en) | 2016-07-29 | 2016-07-29 | Circulation tumor cells autosegregation device |
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| CN205907261U true CN205907261U (en) | 2017-01-25 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085832A (en) * | 2016-07-29 | 2016-11-09 | 深圳市体太糖科技有限公司 | A kind of circulating tumor cell automatic separation system |
| CN108359639A (en) * | 2018-01-24 | 2018-08-03 | 杭州安弼晟生物科技有限公司 | A kind of circulating tumor cell capture systems |
| CN110283714A (en) * | 2019-07-01 | 2019-09-27 | 中国人民解放军陆军军医大学第一附属医院 | Separator is detected based on Terahertz and micro-fluidic circulating tumor cell |
-
2016
- 2016-07-29 CN CN201620821384.8U patent/CN205907261U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085832A (en) * | 2016-07-29 | 2016-11-09 | 深圳市体太糖科技有限公司 | A kind of circulating tumor cell automatic separation system |
| CN108359639A (en) * | 2018-01-24 | 2018-08-03 | 杭州安弼晟生物科技有限公司 | A kind of circulating tumor cell capture systems |
| CN110283714A (en) * | 2019-07-01 | 2019-09-27 | 中国人民解放军陆军军医大学第一附属医院 | Separator is detected based on Terahertz and micro-fluidic circulating tumor cell |
| CN110283714B (en) * | 2019-07-01 | 2022-04-01 | 中国人民解放军陆军军医大学第一附属医院 | Circulating tumor cell detection and separation device based on terahertz and micro-fluidic |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171121 Address after: Two road 518000 Guangdong city of Shenzhen province Nanshan District Xili Street North No. 15 on the third floor of the 301 science and technology Patentee after: SHENZHEN YITI TERAHERTZ TECHNOLOGY CO., LTD. Address before: 518000 Guangdong city of Shenzhen province Nanshan District Xiangshan overseas Chinese overseas Chinese town west two North Road, building six floor 601 10 Maohua Patentee before: One Tai Tang Science and Technology Ltd. of Shenzhen |
|
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 518000 building 3, 5A, Taihua Indus Industrial Park, Baoan District City, Shenzhen, Guangdong. Patentee after: Shenzhen Terahertz Technology Co., Ltd. Address before: 518000 Guangdong, Shenzhen, Nanshan District Xili street, Technology north two Road 15, third floor 301 Patentee before: SHENZHEN YITI TERAHERTZ TECHNOLOGY CO., LTD. |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200915 Address after: Room A601, No.1, MeiXun digital technology factory, No.19, Jinxiu Middle Road, Laokeng community, Longtian street, Pingshan District, Shenzhen City, Guangdong Province Patentee after: SHENZHEN ET MEDICAL TECHNOLOGY Co.,Ltd. Address before: 518000 building 3, 5A, Taihua Indus Industrial Park, Baoan District City, Shenzhen, Guangdong. Patentee before: SHENZHEN TAIHEZI TECHNOLOGY Co.,Ltd. |