CN107142202B - A kind of cell extraction separation method that multifunction automatic biological cell is extracted separation control device and realized using the device - Google Patents
A kind of cell extraction separation method that multifunction automatic biological cell is extracted separation control device and realized using the device Download PDFInfo
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Abstract
A kind of cell extraction separation method that multifunction automatic biological cell is extracted separation control system and realized using the system, is related to blood product technical field.The present invention is to solve the problem of to be easy the cells such as contaminated, blood platelet when extracting sample at present and be easy that the mode low efficiency of therapeutic effect and existing extraction sample is activated and influenced on unsuitable opportunity.Control signal output connects micro machine control signal input to double ECU chips all the way, the driving signal input of micro machine driving signal output end connecting eccentric wheel, eccentric wheel, which rotates clockwise, is clipped in flow-stopping clip on perfusion tube, eccentric wheel, which rotates counterclockwise, opens flow-stopping clip, normal saline bag and biological cell storage bag respectively pass through 1 perfusion tube and import drop pot one end, the drop pot other end connects cell collecting bag and cell filter one end by perfusion tube simultaneously, and the cell filter other end connects syringe injection end and devil liquor recovery bag by perfusion tube simultaneously.It is separated for biological cells.
Description
Technical field
The present invention relates to a kind of biological cells to extract separation control device and control method, belongs to blood product technology neck
Domain.
Background technique
Lymphocyte and platelet rich plasma with the development of laboratory medicine and biotechnology, in monocyte (MNC)
(PRP), the various factors in platelet lysates liquid (PL), are widely used for the multiple fields of scientific research, regenerative therapy.
Mononuclearcell under ultra clean environment by induction, activation, quantity amplification or genetic modification and etc. can be with
Particular kind of immunocyte is obtained, can be used for the adoptive treatment of tumour and targeted therapy.General method is by lymphocyte
Or antigen presenting cell extracts, and by processes such as in vitro culture, modification, activation, differentiation, obtains its quantity and activity greatly
Width improves, and then feeds back to patient's body again, plays antiviral and antitumor action.
Rich in blood platelet technology for treatment of festering caused by bone tissue reparation, burn treating, chronic ulcer and diabetes
Deng playing highly effective facilitation；In terms of beauty, PRP and PL mention color for wrinkle reduction, Firm, removal eye pouch, subtract
Light scar, change pigmentation etc., also play an important role.
So mononuclearcell and the extraction quality rich in platelet products, be the key that influence its function and effect because
Element.
Currently, filtration method also has been used to remove cell from blood, while retaining other blood constituents for then making
With.This method usually in the form of expendable by cell retention in filter, while other blood constituents being allowed to pass through
Filter simultaneously enters collection vessel.For example, filter can be used to remove leucocyte-removing from blood, therefore make of the same race after transfusing blood exempt from
The incidence of epidemic disease reaction minimizes.The typical way that leucocyte removes is carried out using the filter made of plastic fiber net
's.Usually arrange the net that leucocyte is retained in the pseudostructure with adequate thickness, so that cell retention spreads filter
Thickness, therefore keep filter do not block, because if leucocyte is retained in plane surface and will block.
Other than the Physical entrapment of cell, the material and high surface area of filter allow leucocyte irreversibly to adhere to
In surface.In these adherent cells is much exactly the cell thirsted in some medical care precesses.It is generated to retain and be adhered to
The combination of filter establishes the highly efficient process for removing leucocyte-removing before treatment of blood transfusion.However, when leucocyte is the phase
When the cell of prestige, this filter method is no advantage.
Summary of the invention
The present invention is that when extracting sample, the cells such as easy contaminated, blood platelet are easy unsuitable in order to solve at present
Opportunity is activated and influences therapeutic effect, and the problem of the existing mode low efficiency for extracting sample.A kind of more function are now provided
It can automatic biological cell extraction separation control device and the cell extraction separation method using device realization.
A kind of multifunction automatic biological cell extraction separation control device, it includes double ECU chips, micro machine, negative pressure
Isolating device 1, normal saline bag 2, biological cell storage bag 3 and disposable biological cell separation apparatus；
Disposable biological cell separation apparatus includes that 6 current limlitings are pressed from both sides, drop pot 5, cell filter 6, cell filter are fixed
10,6 folder 7, devil liquor recovery bag 8, cell collecting bag 9 and syringe current limliting folders are respectively defined as No.1 current limliting folder 4-1, No. two limits
Stream folder 4-2, No. three current limliting folder 4-3, No. four current limliting folder 4-4, No. five current limlitings press from both sides 4-5 and No. six current limliting and press from both sides 4-6；
Negative-pressure isolation device 1 is body structure, and the negative-pressure cofferdam 1-1 of rectangle is provided in cabinet；
Disposable biological cell separation apparatus is mounted in negative-pressure cofferdam 1-1 and is mounted on postnotum；
Each current limliting folder includes that eccentric wheel 4-1-1, flow-stopping clip 4-1-2 and current limliting press from both sides switch gate 4-1-3；
It is rectangle cover structure that current limliting, which presss from both sides switch gate 4-1-3, and micro machine is fixed on the postnotum inside negative-pressure cofferdam 1-1
On, rectangular cover is fixed on the postnotum of box house, and micro machine, eccentric wheel 4-1-1 and flow-stopping clip 4-1-2 are arranged at square
In shape cover；
Double ECU chips interconnect, and double ECU chips control signal that control signal output connects micro machine all the way inputs
End, the driving signal input of the driving signal output end connecting eccentric wheel 4-1-1 of micro machine, eccentric wheel 4-1-1's is clockwise
Flow-stopping clip 4-1-2 is clipped on perfusion tube by rotation, and flow-stopping clip 4-1-2 is opened in rotating counterclockwise for eccentric wheel 4-1-1；
Normal saline bag 2 and biological cell storage bag 3 respectively import one end of drop pot 5 by 1 perfusion tube, drip the another of pot 5
One end connects one end of cell collecting bag 9 and cell filter 6 by perfusion tube simultaneously, and the other end of cell filter 6 passes through
Perfusion tube connects the injection end and devil liquor recovery bag 8 of syringe 10 simultaneously, and the another way control signal output of double ECU chips connects
Connect the push-in end of syringe 10；
Drop pot 5 is for observing medical fluid flow velocity；
No.1 current limliting folder 4-1 is arranged in normal saline bag 2 and drips on the perfusion tube between pot 5, No. two current limliting folder 4-2 settings
On perfusion tube between biological cell storage bag 3 and drop pot 5, No. three current limliting folder 4-3 settings are in drop pot 5 and cell filter 6
Between perfusion tube on, No. four current limlitings folder 4-4 are arranged on the perfusion tube between cell filter 6 and devil liquor recovery bag 8, No. five
Current limliting folder 4-5 is arranged on the perfusion tube between cell filter 6 and cell collecting bag 9, and No. six current limliting folder 4-6 are arranged in cell
On perfusion tube between filter 6 and syringe 10；
Cell filter 6 is fixed on the backboard of negative-pressure cofferdam 1-1 by cell filter fixing clamp 7.
The invention has the benefit that
Using disposable biological cell separation apparatus and the switch control for combining double ECU chips to press from both sides current limliting, in time, have
Effect neatly changes filtration mode and filtration flow direction, is truly realized multifunction automatic, and be greatly saved physical strength material resources, from
And increase cell filtration efficiency, improve operation accuracy and reduce medical biologic contamination risk, different biologies can be directed to
The advantages of cell characteristics are efficiently filtered.Cell filtration efficiency improves 6 times or more compared with prior art.
The application can with it is different at sleeving plastic multiple-bag together under the action of different cell filtering devices, realize blood
Ingredient separation, cell filtration, blood constituent save integration, prevent blood because opening cause germ contamination risk, avoid because
The loss of biological cell caused by maloperation.
The application is provided with negative-pressure isolation device, in closed environment, is realized by air inlet interface and air draft interface empty
Gas secure exchange, reach particle and microorganism in internal environment continue it is controllable, and using express passway carry out biological cell and
The sterile transfer of medical disposable material, transfer, it is ensured that the protection of operator and biological cell.
Detailed description of the invention
Fig. 1 is the schematic illustration of disposable biological cell separation apparatus；
Fig. 2 is the structural schematic diagram of current limliting folder；
Fig. 3 is the structural schematic diagram for dripping pot, infrared drop pot folder and infrared drop pot folder sensor；
Fig. 4 is the structural schematic diagram of negative-pressure isolation device.
Specific embodiment
Specific embodiment 1: present embodiment is illustrated referring to FIG. 1, FIG. 2 and FIG. 4, one described in present embodiment
Kind of multifunction automatic biological cell extracts separation control device, it include double ECU chips, micro machine, negative-pressure isolation device 1,
Normal saline bag 2, biological cell storage bag 3 and disposable biological cell separation apparatus；
Disposable biological cell separation apparatus includes that 6 current limlitings are pressed from both sides, drop pot 5, cell filter 6, cell filter are fixed
10,6 folder 7, devil liquor recovery bag 8, cell collecting bag 9 and syringe current limliting folders are respectively defined as No.1 current limliting folder 4-1, No. two limits
Stream folder 4-2, No. three current limliting folder 4-3, No. four current limliting folder 4-4, No. five current limlitings press from both sides 4-5 and No. six current limliting and press from both sides 4-6；
Negative-pressure isolation device 1 is body structure, and the negative-pressure cofferdam 1-1 of rectangle is provided in cabinet；
Disposable biological cell separation apparatus is mounted in negative-pressure cofferdam 1-1 and is mounted on postnotum；
Each current limliting folder includes that eccentric wheel 4-1-1, flow-stopping clip 4-1-2 and current limliting press from both sides switch gate 4-1-3；
It is rectangle cover structure that current limliting, which presss from both sides switch gate 4-1-3, and micro machine is fixed on the postnotum inside negative-pressure cofferdam 1-1
On, rectangular cover is fixed on the postnotum of box house, and micro machine, eccentric wheel 4-1-1 and flow-stopping clip 4-1-2 are arranged at square
In shape cover；
Double ECU chips interconnect, and double ECU chips control signal that control signal output connects micro machine all the way inputs
End, the driving signal input of the driving signal output end connecting eccentric wheel 4-1-1 of micro machine, eccentric wheel 4-1-1's is clockwise
Flow-stopping clip 4-1-2 is clipped on perfusion tube by rotation, and flow-stopping clip 4-1-2 is opened in rotating counterclockwise for eccentric wheel 4-1-1；
Normal saline bag 2 and biological cell storage bag 3 respectively import one end of drop pot 5 by 1 perfusion tube, drip the another of pot 5
One end connects one end of cell collecting bag 9 and cell filter 6 by perfusion tube simultaneously, and the other end of cell filter 6 passes through
Perfusion tube connects the injection end and devil liquor recovery bag 8 of syringe 10 simultaneously, and the another way control signal output of double ECU chips connects
Connect the push-in end of syringe 10；
Drop pot 5 is for observing medical fluid flow velocity；
No.1 current limliting folder 4-1 is arranged in normal saline bag 2 and drips on the perfusion tube between pot 5, No. two current limliting folder 4-2 settings
On perfusion tube between biological cell storage bag 3 and drop pot 5, No. three current limliting folder 4-3 settings are in drop pot 5 and cell filter 6
Between perfusion tube on, No. four current limlitings folder 4-4 are arranged on the perfusion tube between cell filter 6 and devil liquor recovery bag 8, No. five
Current limliting folder 4-5 is arranged on the perfusion tube between cell filter 6 and cell collecting bag 9, and No. six current limliting folder 4-6 are arranged in cell
On perfusion tube between filter 6 and syringe 10；
Cell filter 6 is fixed on the backboard of negative-pressure cofferdam 1-1 by cell filter fixing clamp 7.
In present embodiment, disposable biological cell separation apparatus is placed in inside negative-pressure cofferdam 1-1, negative-pressure cofferdam 1-
1 is completely enclosed aseptic processing environment.
Specific embodiment 2: present embodiment is to a kind of biology of multifunction automatic described in specific embodiment one
Cell extraction separation control device is described further, and in present embodiment, each current limliting folder further includes detection sensor 4-1-
4；
Detection sensor 4-1-4 includes pressure sensor or ultrasonic bubble sensor；
Pressure sensor is used to detect the pressure of infusion liquid in pipe；
Ultrasonic bubble sensor is used to detect the bubbles volume and Air Bubble Size of infusion liquid in pipe.
Specific embodiment 3: illustrating present embodiment referring to Fig. 3, present embodiment is to specific embodiment one
A kind of multifunction automatic biological cell extracts separation control device and is described further, and in present embodiment, it is also
Sensor 11 and infrared drop pot folder 12 are pressed from both sides including infrared drop pot；
Infrared drop pot folder 12 presss from both sides 12 tops and is provided with infrared drop pot folder sensor 11 for being clipped in drop pot 5, infrared drop pot,
Infrared drop pot folder sensor 11 is for detecting in drop pot 5 whether have medical fluid instillation.
In this real-time mode, infrared drop pot folder sensor 11 drips in pot 5 for detecting in drop pot 5 whether have medical fluid instillation
Often drip down a drop of liquid, and infrared drop pot folder sensor 11 will detect a signal, and every 20 drop is exactly that 20 signals are
1ml does not detect signal when continuous 2-3 times according to inner parameter setting, will call the police, inform in possible perfusion tube without liquid
?.
Infrared drop pot folder sensor 11 includes infrared drop pot folder transmitting terminal and infrared drop pot clamping connection receiving end, passes through infrared drop pot
Transmitting terminal and infrared drop pot clamping connection receiving end are pressed from both sides to detect the instillation of medical fluid in drop pot 5.
Specific embodiment 4: present embodiment is to a kind of biology of multifunction automatic described in specific embodiment one
Cell extraction separation control device is described further, and in present embodiment, the top of negative-pressure isolation device 1 is provided with air inlet interface
13 and air draft interface 14, LED illumination lamp and ultraviolet sterilizing lamp built in negative-pressure isolation device 1；
Touching display screen 15 is installed on the front panel of negative-pressure isolation device 1, the push button signalling input of touching display screen 15/
Output end connects the push button signalling input/output terminal of double ECU chips, and the front panel of negative-pressure cofferdam 1-1 is transparent glass, transparent
It is driveed on glass there are two hole, which is tightly connected with protection oversleeve gloves 16 respectively, protection oversleeve gloves 16
For to being operated inside negative-pressure isolation device 1.
In present embodiment, it is provided with manhole on the postnotum outside negative-pressure isolation device 1, for overhauling negative pressure isolation
Device 1.
Specific embodiment 5: present embodiment is to a kind of biology of multifunction automatic described in specific embodiment four
Cell extraction separation control device is described further, and it is sterile to be additionally provided with negative pressure in present embodiment, in negative-pressure isolation device 1
Transferring cabin 1-2 is provided with a transmission channels door 17 between negative pressure sterile transfer cabin 1-2 and negative-pressure cofferdam 1-1.
In present embodiment, protection oversleeve gloves 16 are taken, in an aseptic environment, by normal saline bag 2, biological cell
Storage bag 3 and disposable biological cell separation apparatus are moved in negative-pressure cofferdam 1-1 from negative pressure sterile transfer cabin 1-2.
Specific embodiment 6: present embodiment is to a kind of biology of multifunction automatic described in specific embodiment five
Cell extraction separation control device is described further, and in present embodiment, it further includes warning device；
The inductive signal output end of infrared drop pot folder sensor 11, the inductive signal output end of detection sensor 4-1-4 divide
Not Lian Jie double ECU chips control signal input, the control signal output of double ECU chips is separately connected the report of warning device
Alert signal input part.
In present embodiment, the purpose using double ECU chips is to prevent an ECU wafer damage, can with another into
Row operation, does not influence normally to operate.The collected value output of sensor is to double ECU chips, the setting with double ECU chip interiors
Value is compared, if it is not the same, then alarming, and alarm signal is shown in touch display screen.
Specific embodiment 7: present embodiment is raw using a kind of multifunction automatic described in specific embodiment five
The biological cell that object cell extraction separation control device is realized extracts separation control method, in present embodiment, the method packet
Include following steps:
Step 1: opening biological cell extracts separator, opens ultraviolet sterilizing lamp and carry out ultraviolet sterilization function
30min；
Step 2: ultraviolet sterilization lamp is closed, by normal saline bag 2, biological cell storage bag 3 and disposable biological cell point
It is placed on inside negative pressure sterile transfer cabin 1-2 from device through surface sterilizing；
Step 3: normal saline bag 2, biological cell are stored by transmission channels door 17 with oversleeve gloves 16 using protection
Bag 3 and disposable biological cell separation apparatus are moved in negative-pressure cofferdam 1-1 from negative pressure sterile transfer cabin 1-2, then will be given birth to
Reason bag of saline 2, biological cell storage bag 3 and disposable biological cell separation apparatus are installed to the inside back of negative-pressure cofferdam 1-1
On plate, and sufficiently exclude the liquid level in drop pot 5；
Step 4: carrying out flushing of pipeline: 4-3 and No. four control No.1 current limliting folder 4-1, No. three current limlitings folders current limliting folder 4-4 are opened
It opens, other current limliting clip closures, the salt water in normal saline bag 2 flows through drop pot 5 and cell filter 6, into devil liquor recovery bag 8；
Step 5: carrying out cell forward filter: control No. two current limliting folder 4-2, No. three current limlitings press from both sides 4-3 and No. four current limliting and press from both sides 4-
4 open, other current limliting clip closures, and the blood in biological cell storage bag 3 flows through drop pot 5 and cell filter 6, return into waste liquid
Closing bag 8；
Reversely collect Step 6: carrying out cell: No. five current limlitings of control press from both sides 4-5 and No. six current limliting folder 4-6 unlatching, other current limlitings
Clip closure, control 10 inverse injection reagent of syringe flow through cell filter 6, into cell collecting bag 9, realize cell extraction point
From.
Embodiment 1:
The biological cell that separation control device is realized, which is extracted, according to a kind of multifunction automatic biological cell extracts separation control
Method processed changes syringe 10 into cell collecting bag, the described method comprises the following steps:
Step 1: opening biological cell extracts separation system, opens ultraviolet sterilizing lamp and carry out ultraviolet sterilization function
30min；
Step 2: ultraviolet sterilization lamp is closed, by normal saline bag 2, biological cell storage bag 3 and disposable biological cell point
It is placed on inside negative pressure sterile transfer cabin 1-2 from device through surface sterilizing；
Step 3: normal saline bag 2, biological cell are stored by transmission channels door 17 with oversleeve gloves 16 using protection
Bag 3 and disposable biological cell separation apparatus are moved in negative-pressure cofferdam 1-1 from negative pressure sterile transfer cabin 1-2, then will be given birth to
Reason bag of saline 2, biological cell storage bag 3 and disposable biological cell separation apparatus are installed to the inside back of negative-pressure cofferdam 1-1
On plate, and sufficiently exclude the liquid level in drop pot 5；
Step 4: carrying out flushing of pipeline: 4-3 and No. four control No.1 current limliting folder 4-1, No. three current limlitings folders current limliting folder 4-4 are opened
It opens, other current limliting clip closures, the salt water in normal saline bag 2 flows through drop pot 5 and cell filter 6, into devil liquor recovery bag 8；
Step 5: carrying out cell forward filter: control No. two current limliting folder 4-2, No. three current limlitings press from both sides 4-3 and No. six current limliting and press from both sides 4-
6 open, other current limliting clip closures, and the blood in biological cell storage bag 3 flows through drop pot 5 and cell filter 6, receive into cell
Collect bag, realizes that cell is collected.
Embodiment 2:
The biological cell that separation control device is realized, which is extracted, according to a kind of multifunction automatic biological cell extracts separation control
Method processed, the described method comprises the following steps:
Step 1: opening biological cell extracts separator, opens ultraviolet sterilizing lamp and carry out ultraviolet sterilization function
30min；
Step 2: ultraviolet sterilization lamp is closed, by normal saline bag 2, biological cell storage bag 3 and disposable biological cell point
It is placed on inside negative pressure sterile transfer cabin 1-2 from device through surface sterilizing；
Step 3: normal saline bag 2, biological cell are stored by transmission channels door 17 with oversleeve gloves 16 using protection
Bag 3 and disposable biological cell separation apparatus are moved in negative-pressure cofferdam 1-1 from negative pressure sterile transfer cabin 1-2, then will be given birth to
Reason bag of saline 2, biological cell storage bag 3 and disposable biological cell separation apparatus are installed to the inside back of negative-pressure cofferdam 1-1
On plate, and sufficiently exclude the liquid level in drop pot 5；
Step 4: carrying out flushing of pipeline: 4-3 and No. four control No.1 current limliting folder 4-1, No. three current limlitings folders current limliting folder 4-4 are opened
It opens, other current limliting clip closures, the salt water in normal saline bag 2 flows through drop pot 5 and cell filter 6, into devil liquor recovery bag 8；
Step 5: carrying out cell forward filter: No. five current limlitings of control press from both sides 4-5 and No. six current limliting folder 4-6 unlatching, other current limlitings
Folder 4 is closed, and control syringe 10 injects reagent and flows through cell filter 6, into cell collecting bag 9, is then extracted as needed
Sample is detected in cell collecting bag 9.
Claims (7)
1. a kind of multifunction automatic biological cell extracts separation control device, which is characterized in that it includes double ECU chips, micro-
Motor, negative-pressure isolation device (1), normal saline bag (2), biological cell storage bag (3) and disposable biological cell separation apparatus,
Disposable biological cell separation apparatus includes that 6 current limliting folders, drop pot (5), cell filter (6), cell filters are fixed
(7), devil liquor recovery bag (8), cell collecting bag (9) and syringe (10) are pressed from both sides, 6 current limliting folders are respectively defined as No.1 current limliting folder
(4-1), No. two current limliting folders (4-2), No. three current limliting folders (4-3), No. four current limliting folders (4-4), No. five current limliting folders (4-5) and No. six limits
Stream folder (4-6),
Negative-pressure isolation device (1) is body structure, and the negative-pressure cofferdam (1-1) of rectangle is provided in cabinet,
Disposable biological cell separation apparatus is mounted in negative-pressure cofferdam (1-1) and is mounted on postnotum,
Each current limliting folder includes that eccentric wheel (4-1-1), flow-stopping clip (4-1-2) and current limliting press from both sides switch gate (4-1-3),
It is rectangle cover structure that current limliting, which presss from both sides switch gate (4-1-3), and micro machine is fixed on the internal postnotum of negative-pressure cofferdam (1-1)
On, rectangular cover is fixed on the postnotum of box house, and micro machine, eccentric wheel (4-1-1) and flow-stopping clip (4-1-2) are respectively provided with
In rectangular cover,
Double ECU chips interconnect, the control signal input of double ECU chips control signal output connection micro machine all the way,
The driving signal input of the driving signal output end connecting eccentric wheel (4-1-1) of micro machine, eccentric wheel (4-1-1) it is clockwise
Flow-stopping clip (4-1-2) is clipped on perfusion tube by rotation, and flow-stopping clip (4-1-2) is opened in rotating counterclockwise for eccentric wheel (4-1-1),
Normal saline bag (2) and biological cell storage bag (3) respectively import one end of drop pot (5) by 1 perfusion tube, drip pot (5)
The other end one end of cell collecting bag (9) and cell filter (6) is connected simultaneously by perfusion tube, cell filter (6)
The other end connects the injection end and devil liquor recovery bag (8) of syringe (10), the another way control of double ECU chips by perfusion tube simultaneously
The push-in end of signal output end connection syringe (10) processed,
Drop pot (5) is used to observe medical fluid flow velocity,
No.1 current limliting presss from both sides (4-1) setting on the perfusion tube between normal saline bag (2) and drop pot (5), and No. two current limlitings press from both sides (4-2)
Be arranged on the perfusion tube between biological cell storage bag (3) and drop pot (5), No. three current limlitings folder (4-3) setting drop pot (5) with
On perfusion tube between cell filter (6), No. four current limliting folders (4-4) are arranged in cell filter (6) and devil liquor recovery bag (8)
Between perfusion tube on, perfusion tube that No. five current limlitings folder (4-5) is arranged between cell filter (6) and cell collecting bag (9)
On, on the perfusion tube that No. six current limliting folders (4-6) are arranged between cell filter (6) and syringe (10),
Cell filter (6) is fixed on the backboard of negative-pressure cofferdam (1-1) by cell filter fixing clamp (7)；
Carry out cell forward filter: No. two current limliting folders (4-2) of control, No. three current limliting folders (4-3) and No. four current limliting folders (4-4) are opened
It opens, other current limliting clip closures, the blood in biological cell storage bag (3) flows through drop pot (5) and cell filter (6), into giving up
Liquid collection bag (8)；
Carry out cell reversely to collect: No. five current limliting folders (4-5) of control and No. six current limliting folders (4-6) are opened, other current limliting clip closures,
Control syringe (10) inverse injection reagent flows through cell filter (6), into cell collecting bag (9), realizes cell extraction point
From.
2. a kind of multifunction automatic biological cell according to claim 1 extracts separation control device, which is characterized in that
Each current limliting folder further includes detection sensor (4-1-4),
Detection sensor (4-1-4) includes pressure sensor or ultrasonic bubble sensor,
Pressure sensor is used to detect the pressure of infusion liquid in pipe；
Ultrasonic bubble sensor is used to detect the bubbles volume and Air Bubble Size of infusion liquid in pipe.
3. a kind of multifunction automatic biological cell according to claim 1 extracts separation control device, which is characterized in that
It further includes that infrared drop pot folder sensor (11) and infrared drop pot press from both sides (12),
Infrared drop pot folder (12) is provided with infrared drop pot folder sensor for being clipped in drop pot (5), infrared drop pot folder (12) top
(11), infrared drop pot folder sensor (11) is for detecting in drop pot (5) whether have medical fluid instillation.
4. a kind of multifunction automatic biological cell according to claim 1 extracts separation control device, which is characterized in that
The top of negative-pressure isolation device (1) is provided with air inlet interface (13) and air draft interface (14), and LED built in negative-pressure isolation device (1) shines
Bright lamp and ultraviolet sterilizing lamp,
It is equipped on the front panel of negative-pressure isolation device (1) touching display screen (15), the push button signalling of touching display screen (15) is defeated
Enter/output end connects the push button signalling input/output terminals of double ECU chips, the front panel of negative-pressure cofferdam (1-1) is transparent glass
Glass is driveed on transparent glass there are two hole, which is tightly connected with a protection with oversleeve gloves (16) respectively, protection sleeve
Set gloves (16) is used for operate internal to negative-pressure isolation device (1).
5. a kind of multifunction automatic biological cell according to claim 4 extracts separation control device, which is characterized in that
Negative pressure sterile transfer cabin (1-2), negative pressure sterile transfer cabin (1-2) and negative-pressure cofferdam are additionally provided in negative-pressure isolation device (1)
A transmission channels door (17) is provided between (1-1).
6. a kind of multifunction automatic biological cell according to claim 5 extracts separation control device, which is characterized in that
It further includes warning device,
The inductive signal output end of infrared drop pot folder sensor (11), the inductive signal output end of detection sensor (4-1-4) divide
Not Lian Jie double ECU chips control signal input, the control signal output of double ECU chips is separately connected the report of warning device
Alert signal input part.
7. it is thin to extract the biology that separation control device is realized using a kind of multifunction automatic biological cell described in claim 5
Born of the same parents extract separation control method, which is characterized in that the described method comprises the following steps:
Step 1: opening biological cell extracts separator, opens ultraviolet sterilizing lamp and carry out ultraviolet sterilization function 30min；
Step 2: ultraviolet sterilization lamp is closed, by normal saline bag (2), biological cell storage bag (3) and disposable biological cell point
Negative pressure sterile transfer cabin (1-2) inside is placed on through surface sterilizing from device；
Step 3: normal saline bag (2), biological cell are deposited by transmission channels door (17) with oversleeve gloves (16) using protection
Storage bag (3) and disposable biological cell separation apparatus are moved in negative-pressure cofferdam (1-1) from negative pressure sterile transfer cabin (1-2),
Then normal saline bag (2), biological cell storage bag (3) and disposable biological cell separation apparatus are installed to negative-pressure cofferdam
On the inside backboard of (1-1)；
Step 4: carrying out flushing of pipeline: control No.1 current limliting folder (4-1), No. three current limliting folders (4-3) and No. four current limliting folders (4-4)
It opens, other current limliting clip closures, the salt water in normal saline bag (2) flows through drop pot (5) and cell filter (6), into waste liquid
Collection bag (8)；
Step 5: carrying out cell forward filter: No. two current limliting folders (4-2) of control, No. three current limliting folders (4-3) and No. four current limliting folders
(4-4) is opened, other current limliting clip closures, and the blood in biological cell storage bag (3) flows through drop pot (5) and cell filter (6),
Into devil liquor recovery bag (8)；
Reversely collect Step 6: carrying out cell: No. five current limliting folders (4-5) of control and No. six current limliting folders (4-6) are opened, other current limlitings
Clip closure, control syringe (10) inverse injection reagent flow through cell filter (6), into cell collecting bag (9), realize cell
Extract separation.
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| CN113509607B (en) * | 2021-04-23 | 2023-04-18 | 温州医科大学附属第一医院 | Pulmonary artery high-pressure cardiac hybrid radiography auxiliary device |
| WO2023241597A1 (en) * | 2022-06-14 | 2023-12-21 | 南京金斯瑞生物科技有限公司 | Pipeline system for cell sorting, cell sorting device, and control method |
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| CN102971411A (en) * | 2010-07-01 | 2013-03-13 | 株式会社钟化 | Disposable set for cell culture, cell culture device and cell preparation method |
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| CN102971411A (en) * | 2010-07-01 | 2013-03-13 | 株式会社钟化 | Disposable set for cell culture, cell culture device and cell preparation method |
| CN201883098U (en) * | 2010-12-01 | 2011-06-29 | 汪华 | Automatic production device for medical cells |
| CN105498021A (en) * | 2014-09-26 | 2016-04-20 | 无锡市贝尔康电子研究所 | Medical intravenous injection system with real-time monitoring and timing control functions |
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