CN108535068A - A kind of cytology DNA detections Sample preservation liquid pretreatment unit - Google Patents
A kind of cytology DNA detections Sample preservation liquid pretreatment unit Download PDFInfo
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- CN108535068A CN108535068A CN201810301599.0A CN201810301599A CN108535068A CN 108535068 A CN108535068 A CN 108535068A CN 201810301599 A CN201810301599 A CN 201810301599A CN 108535068 A CN108535068 A CN 108535068A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A kind of cytology DNA detections Sample preservation liquid pretreatment unit.The present invention relates to a kind of pretreatment units for holding cell-preservation liquid for liquid-based cell sample manufacturing.More particularly to a kind of guarantor's storage bottle for realizing the impurity in double medium filtration and removal sample, inflammatory cell, red blood cell, Mini Tissue cell.It is made of plastic bottle body, prefiltration net, two layers of filtering net film, bottle cap and suction pipe, the invention can realize that liquid basal cell is fast and safely effectively filtered and screened.Wherein：Prefiltration screen membrane filters out impurity and mucus, two layers of filtering net film have filtered out inflammatory cell, red blood cell, Mini Tissue cell, liquid basal cell effectively filter and screen, the epithelial cell of diagnostic significance can be more effectively enriched with, improve the slice-making quality of liquid basal cell sample, the more preferable generation for avoiding false negative, ensure that the accuracy of inspection.
Description
Technical field
The present invention relates to a kind of cytology DNA to detect Sample preservation liquid pretreatment unit.It is widely used in and falls off carefully
Born of the same parents learn, more particularly to it is a kind of realizes double medium filtration and removal sample in impurity, filter out inflammatory cell, red blood cell, small-sized group
Knit guarantor's storage bottle of cell.It can realize that liquid basal cell is fast and safely effectively filtered and screened, can more effectively be enriched with
The epithelial cell of diagnostic significance improves the slice-making quality of liquid basal cell sample.
Background technology
Cervical carcinoma is multiple one of the malignant tumour of women, cervical carcinoma be can early find, early treatment, can cure one
Kind disease.TCT is mainly used for the screening of cervical carcinoma, and the process of domestic liquid-based cell sample manufacturing is all realized by hand, and what is used is heavy
Drop plate, feed bin safety is poor, needs to overturn when removing liquid in feed bin and topple over, easy cross contamination, and safety is poor.
The screening of cast-off cells includes following operation：It is sampled using sample-taking brush, protects in storage bottle and preserved, vibrated
And it is uniformly dissolved, Thinprep pap test film-making and dye piece, microscopically observation.Flaking method based on manual film-making, including from
Heart sedimentation, membrane type film-making etc., centrifugal settling method are a kind of current medical most commonly used cast-off cells film-makers of testing agency
Method.
The exfoliated cell preservative fluid bottle of centrifugal settling method film-making at present is the plastic bottle of not filter screen mostly, after sampling
Sample-taking brush, which is directly placed into, to be preserved in liquid plastic bottle, and liquid basal cell, which is eluted in, to be preserved in liquid, and impurity and mucus etc. are with cast-off cells
It is attached on glass slide, influences slice-making quality and sees the observation and analytical judgment of piece doctor.
Prefiltration nethike embrane of the present invention can effectively impurity screening and mucus, it is only necessary to which normally oscillation dissolving can be realized, side
Convenient to operate；Synchronous two layers of filtering net film can filter out inflammatory cell, red blood cell, Mini Tissue cell, can more effectively be enriched with
The epithelial cell for having diagnostic significance improves the slice-making quality of liquid basal cell sample, it is ensured that film-making and dye tablet quality.
Invention content
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4, Fig. 5, Fig. 6, a kind of cytology DNA detections Sample preservation liquid pretreatment unit.
More particularly to a kind of guarantor realizing the impurity in double medium filtration and removal sample, inflammatory cell, red blood cell, Mini Tissue cell
Storage bottle.It is made of plastic bottle body, prefiltration net, two layers of filtering net film, bottle cap and suction pipe, the invention major function part is first mistake
Screen membrane and two layers of filtering net film, centre are provided with suction pipe, and the extension position of suction pipe is in the intermediate region of two membranes, prefiltration
Nethike embrane can realize that liquid basal cell is fast and safely effectively filtered and screened, and two layers of filtering net film filter out inflammation with can having handing-over
Cell, red blood cell, Mini Tissue cell, leave useful liquid basal cell, and the outlet of suction pipe is located at useful liquid basal cell region.
The prefiltration screen membrane mesh number 120-150 mesh of the cell-preservation liquid bottle of double film layer functions filters out impurity and glues
Liquid；Two layers of 1000 mesh of filtering net film mesh number, have filtered out inflammatory cell, red blood cell, Mini Tissue cell；Liquid basal cell is carried out
It effectively filters and screens, can more effectively be enriched with the epithelial cell of diagnostic significance, improve the system of liquid basal cell sample
Tablet quality, while ensure that the accuracy of inspection.
Description of the drawings
Fig. 1 is graphics after plastic bottle body of the present invention is transparent
Fig. 2 is graphics after plastic bottle body of the present invention is transparent
Fig. 3 is three dimensional sectional view of the present invention
Fig. 4 is front view of the present invention
Fig. 5 is the sectional view of left view of the present invention
Fig. 6 is the sectional view that the present invention looks up direction
1. plastic bottle body；2. two layers of filtering net film；3. prefiltration net；4. bottle cap；5. suction pipe.
Liquid in bottle is divided into three regions：Contain impurity and mucus, the promising useful liquid in middle level in the prefiltration nethike embrane of upper layer
Basal cell contains inflammatory cell, red blood cell, Mini Tissue cell below two layers of filtering net film.
Specific implementation mode
Doctor is sampled using sample-taking brush, sample-taking brush with sample be put into protect storage bottle in preserved, preserve liquid into
Row pretreatment, acquisition have the specimen preserving liquid bottle of cell sample to be transmitted to laboratory, and laboratory operation personnel receive guarantor's storage bottle
When, vibrated and be uniformly dissolved, it is no longer necessary to add separating liquid and cleaning solution, need not be shaken again and centrifuge with it is quiet
It sets, can directly take middle level to there is the epithelial cell of diagnostic significance to carry out Thinprep pap test film-making and dye piece, system is greatly saved
The piece time reduces the labor intensity of manual film-making, improves work efficiency, while better ensuring that the quality control process of sample.
Claims (4)
1. a kind of cytology DNA detects Sample preservation liquid pretreatment unit, which is characterized in that including plastic bottle body, prefiltration net,
Two layers of filtering net film, bottle cap and suction pipe can be realized effectively and filter out impurity and mucus.
2. a kind of cytology DNA according to claim 1 detects Sample preservation liquid pretreatment unit, it is characterised in that first mistake
Screen membrane mesh number 120-150 mesh can realize that impurity and mucus are acted in filtering and removal sample with a step, can be more effectively rich
Collection has the epithelial cell of diagnostic significance, improves liquid basal cell quality, promotes Thinprep pap test slice-making quality.
3. a kind of cytology DNA according to claim 2 detects Sample preservation liquid pretreatment unit, it is characterised in that according to
The smaller feature of inflammatory cell, red blood cell, Mini Tissue cell dia, two layers of 1000 mesh of filtering net film mesh number, can be effective
Inflammatory cell, red blood cell, Mini Tissue cell are filtered out, useful liquid basal cell is retained, effectively avoids inflammatory cell, red blood cell, small
Type histocyte interferes the judgement of doctor, ensures the quality of Thinprep pap test smear sedimentation.
4. a kind of cytology DNA according to claim 2 detects Sample preservation liquid pretreatment unit, suction pipe position corresponds to
Liquid is effective liquid basal cell after the filtering of prefiltration nethike embrane and two layers of filtering net film filter out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810301599.0A CN108535068A (en) | 2018-04-04 | 2018-04-04 | A kind of cytology DNA detections Sample preservation liquid pretreatment unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810301599.0A CN108535068A (en) | 2018-04-04 | 2018-04-04 | A kind of cytology DNA detections Sample preservation liquid pretreatment unit |
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| CN108535068A true CN108535068A (en) | 2018-09-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810301599.0A Pending CN108535068A (en) | 2018-04-04 | 2018-04-04 | A kind of cytology DNA detections Sample preservation liquid pretreatment unit |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109358028A (en) * | 2018-11-09 | 2019-02-19 | 江西业力医疗器械有限公司 | A kind of two Methods for Fungi Detection based on liquid-based tabletting technology and fluorescent staining technique |
| CN111238889A (en) * | 2020-01-16 | 2020-06-05 | 江西业力医疗器械有限公司 | Liquid-based fungus detection method based on flaking method |
| CN112113817A (en) * | 2020-10-09 | 2020-12-22 | 嘉兴晶铸生物科技有限公司 | Full-automatic exfoliated cell slide making method |
| CN112126590A (en) * | 2020-10-28 | 2020-12-25 | 北京中源合聚生物科技有限公司 | Cell adherent microcarrier inoculation device and method |
| US12584827B2 (en) | 2020-10-09 | 2026-03-24 | Jiaxing Jingzhu Biotechnology Co., Ltd. | Full-automatic preparation method of cast-off cell smear |
-
2018
- 2018-04-04 CN CN201810301599.0A patent/CN108535068A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109358028A (en) * | 2018-11-09 | 2019-02-19 | 江西业力医疗器械有限公司 | A kind of two Methods for Fungi Detection based on liquid-based tabletting technology and fluorescent staining technique |
| CN111238889A (en) * | 2020-01-16 | 2020-06-05 | 江西业力医疗器械有限公司 | Liquid-based fungus detection method based on flaking method |
| CN112113817A (en) * | 2020-10-09 | 2020-12-22 | 嘉兴晶铸生物科技有限公司 | Full-automatic exfoliated cell slide making method |
| CN112113817B (en) * | 2020-10-09 | 2023-03-14 | 嘉兴晶铸生物科技有限公司 | Full-automatic exfoliated cell slide making method |
| US12584827B2 (en) | 2020-10-09 | 2026-03-24 | Jiaxing Jingzhu Biotechnology Co., Ltd. | Full-automatic preparation method of cast-off cell smear |
| CN112126590A (en) * | 2020-10-28 | 2020-12-25 | 北京中源合聚生物科技有限公司 | Cell adherent microcarrier inoculation device and method |
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Application publication date: 20180914 |