CN203999608U - A kind of biological specimen cascade filtration and nucleic acid purification device - Google Patents
A kind of biological specimen cascade filtration and nucleic acid purification device Download PDFInfo
- Publication number
- CN203999608U CN203999608U CN201420316267.7U CN201420316267U CN203999608U CN 203999608 U CN203999608 U CN 203999608U CN 201420316267 U CN201420316267 U CN 201420316267U CN 203999608 U CN203999608 U CN 203999608U
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- nucleic acid
- column
- biological specimen
- acid purification
- purification device
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- 238000001914 filtration Methods 0.000 title claims abstract description 56
- 238000001821 nucleic acid purification Methods 0.000 title claims abstract description 40
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 55
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 55
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 55
- 238000001179 sorption measurement Methods 0.000 claims abstract description 54
- 239000012528 membrane Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 description 7
- 239000011148 porous material Substances 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002365 multiple layer Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a kind of biological specimen cascade filtration and nucleic acid purification device, belongs to bio-filter technology field, and it has solved the problem that filtration unit of the prior art cannot complete filtration and nucleic acid purification simultaneously.This biological specimen cascade filtration and nucleic acid purification device, comprise the Filter column being also provided with on nucleic acid adsorption column and nucleic acid adsorption column, and Filter column is socketed in nucleic acid adsorption column.This biological specimen cascade filtration and nucleic acid purification device utilize Filter column to filter, utilize nucleic acid adsorption column to carry out nucleic acid purification, can reach easily the object of filtration and the integrated operation of nucleic acid purification, realize biological specimen filtration and nucleic acid purification and carry out simultaneously.
Description
Technical field
The utility model belongs to bio-filter technology field, relates to a kind of biological specimen filter device, particularly a kind of biological specimen cascade filtration and nucleic acid purification device.
Background technology
Filtration is conventional technical process in chemistry, biology, in existing filtering technique, filtering apparatus can only use the filtering material in single aperture, when needs obtain the different purification effect requiring, often needs filtering material filter 23～5 time of different pore size; In the adsorption process of prior art, target product is separated with impurity, need to remove filtrate residual, to scavenging solution be pressed in adsorption medium with pressure pump, if while changing washing lotion or target product wash-out, just cannot operate continuously, while changing instrument, vacuum state is destroyed, and adsorption effect is poor.
In addition, biological specimen carries out should carrying out cascade filtration before nucleic acid purification, after filtering, carry out again nucleic acid absorption to reach purification effect, what yet in prior art, filtration and purifying adopted is different equipment, in transfer process, easily produce contaminating impurity, and handoff procedure is comparatively loaded down with trivial details, and purification efficiency is low.
Summary of the invention
The purpose of this utility model is to have the problems referred to above for existing technology, has proposed a kind of filtration unit that has biological specimen filtration and nucleic acid purification function concurrently.
The purpose of this utility model can realize by following technical proposal: a kind of biological specimen cascade filtration and nucleic acid purification device, comprise nucleic acid adsorption column, it is characterized in that, on described nucleic acid adsorption column, be also provided with Filter column, described Filter column is socketed in nucleic acid adsorption column.
Utilize Filter column to filter, nucleic acid adsorption column carries out nucleic acid absorption, can reach easily the object of filtration and the integrated operation of nucleic acid purification, realizes biological specimen filtration and nucleic acid purification and carries out simultaneously, raises the efficiency.
In above-mentioned biological specimen cascade filtration and nucleic acid purification device, described Filter column outside is provided with outside screw, and described nucleic acid adsorption column inwall is provided with internal thread, and described Filter column is threaded with nucleic acid adsorption column.
In above-mentioned biological specimen cascade filtration and nucleic acid purification device, described Filter column is a plurality of and can mutually superposes, and Filter column outside is provided with outside screw, between each Filter column and next Filter column, is and is threaded.
In above-mentioned biological specimen cascade filtration and nucleic acid purification device, described nucleic acid adsorption column is provided with internal thread, and the clamping mouth that has been threaded below described nucleic acid adsorption column, is provided with nucleic acid adsorption film in described clamping mouth.
In above-mentioned biological specimen cascade filtration and nucleic acid purification device, described nucleic acid adsorption film is provided with compacting ring, and described clamping gonys portion is provided with discharge port.Clamping mouth is removably connected to nucleic acid adsorption column bottom, utilizes this clamping mouth to can be used for being connected with container, also can be used for being arranged on centrifuge tube, carrying out centrifugal treating.
In above-mentioned biological specimen cascade filtration and nucleic acid purification device, described Filter column comprises the filtering membrane that shaft and shaft bottom arrange, and described filtering membrane is provided with compacting ring, and described Filter column bottom is provided with discharge port.By selecting the filtering membrane of different pore size, realize cascade filtration.
In above-mentioned biological specimen cascade filtration and nucleic acid purification device, described clamping mouth is provided with for the clamping shirt rim with the clamping of centrifuge tube inwall.
Compared with prior art, this biological specimen cascade filtration and nucleic acid purification device utilize Filter column to filter, utilize nucleic acid adsorption column to carry out nucleic acid purification, can reach easily the object of filtration and the integrated operation of nucleic acid purification, realize biological specimen filtration and nucleic acid purification carries out simultaneously, in addition, Filter column also can multiple-layer stacked, realize the filtration of different pore size, also can realize a plurality of samples processes simultaneously, raise the efficiency, the second, Filter column and nucleic acid adsorption column, can distinguish large, medium and small three kinds of specifications, to meet the demand of different samples; The 3rd, the pore size of three kinds of specification nucleic acid adsorption column bottom discharge ports is the same, can connect vacuum unit, also may be rotated down, and is positioned in the centrifugal body of 1.5mL or 2.0mL, carries out high speed centrifugation, raises the efficiency.
Accompanying drawing explanation
Fig. 1 is the sectional structure schematic diagram of the present embodiment 1;
Fig. 2 is the use view of the present embodiment 1;
Fig. 3 is another use view of the present embodiment 1;
Fig. 4 is the use state graph of the present embodiment 2.
In figure, 1, sole plate; 2, nucleic acid adsorption column; 21, nucleic acid adsorption film; 22, compacting ring; 23, discharge port; 24, clamping mouth; 25, clamping shirt rim; 26, upper cover; 3, Filter column; 4, outside screw; 5, internal thread; 6, centrifuge tube.
Embodiment
Be below specific embodiment of the utility model by reference to the accompanying drawings, the technical solution of the utility model is further described, but the utility model be not limited to these embodiment.
Embodiment 1
As depicted in figs. 1 and 2, this biological specimen cascade filtration and nucleic acid purification device, comprise nucleic acid adsorption column 2 and the sole plate 1 being fixedly connected with nucleic acid adsorption column 2, is also provided with Filter column 3 on nucleic acid adsorption column 2, and Filter column 3 is socketed in nucleic acid adsorption column 2.Utilize Filter column 3 to filter, nucleic acid adsorption column 2 carries out nucleic acid absorption, can reach easily the object of filtration and the integrated operation of nucleic acid purification, realizes biological specimen filtration and nucleic acid purification and carries out simultaneously, raises the efficiency.Filter column 3 outsides are provided with outside screw 4, and described nucleic acid adsorption column 2 inwalls are provided with internal thread 5, and described Filter column 3 is threaded with nucleic acid adsorption column 2.Filter column 3 is for a plurality of and can mutually superpose, and Filter column 3 outsides are provided with outside screw 4, between each Filter column 3 and next Filter column 3, is and is threaded.Nucleic acid adsorption column 2 is provided with internal thread, and the described nucleic acid adsorption column 2 belows clamping mouth 24 that has been threaded, is provided with nucleic acid adsorption film 21 in described clamping mouth 24.Nucleic acid adsorption film 21 is provided with compacting ring 22, and described clamping mouth 24 bottoms are provided with discharge port 23.Clamping mouth 24 is removably connected to nucleic acid adsorption column 2 bottoms, utilizes this clamping mouth 24 to can be used for being connected with container 1, also can be used for being arranged on centrifuge tube carrying out centrifugal treating, by selecting the filtering membrane 31 of different pore size to realize cascade filtration.
This biological specimen cascade filtration and nucleic acid purification device are for filtering biological specimen and nucleic acid absorption, utilize Filter column 3 to filter, utilize nucleic acid adsorption column 2 to carry out nucleic acid purification, can reach easily the object of filtration and the integrated operation of nucleic acid purification, realize biological specimen filtration and nucleic acid purification carries out simultaneously, in addition, Filter column 3 also can multiple-layer stacked, realizes the filtration of different pore size, when can realize a plurality of biological specimen, filter, raise the efficiency.
Be illustrated in figure 2 the use state graph of this device, utilize Filter column 3 to be socketed on nucleic acid adsorption column 2, the clamping mouth 24 of nucleic acid adsorption column 2 is installed on sole plate 1, and sole plate 1 is for accepting biological specimen, for improving nucleic acid quality of adsorption, stackable a plurality of Filter columns 3 carry out classified filtering.Meanwhile, for improving sample process efficiency, can on sole plate 1, place a plurality of nucleic acid purification devices and filter and nucleic acid purification simultaneously.
Be illustrated in figure 3 another use state graph of this device, the discharge port 23 of the Filter column 3 clamping mouth 24 that has been threaded, by Filter column 3 by pending sample be contained in clamping mouth 24 interior after, utilize the upper cover 26 of clamping mouth 24 that sample is covered in clamping mouth, then the clamping shirt rim 25 on clamping mouth 24 is connected with centrifuge tube 6 inwalls, put into whizzer and carry out centrifugal treating, reach the effect of fast purifying.
Embodiment 2
Be another embodiment of this device as described in Figure 4, its structure and principle of work are substantially the same manner as Example 1, different places are, the size of the nucleic acid adsorption column 2 of the present embodiment can directly be placed in the centrifuge tube of 1.5 mL or 2.0 mL, therefore without clamping mouth, nucleic acid adsorption film 21 is positioned at nucleic acid adsorption column bottom, and compress nucleic acid adsorption film 21 by compacting ring 22, nucleic acid adsorption column 2 is directly socketed on 1.5ml or 2.0ml centrifuge tube 6, sample to be filtered is contained in upper cover 26 on the interior bonnet of nucleic acid adsorption column, puts into whizzer and carries out fast purifying.
Specific embodiment described herein is only to the explanation for example of the utility model spirit.The utility model person of ordinary skill in the field can make various modifications or supplements or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present utility model or surmount the defined scope of appended claims.
Claims (6)
1. a biological specimen cascade filtration and nucleic acid purification device, comprise nucleic acid adsorption column, it is characterized in that, on described nucleic acid adsorption column, be also provided with Filter column, described Filter column is socketed in nucleic acid adsorption column, described Filter column outside is provided with outside screw, and described nucleic acid adsorption column inwall is provided with internal thread, and described Filter column is threaded with nucleic acid adsorption column.
2. biological specimen cascade filtration according to claim 1 and nucleic acid purification device, is characterized in that, described Filter column is a plurality of and can mutually superposes, and Filter column outside is provided with outside screw, between each Filter column and next Filter column, is and is threaded.
3. biological specimen cascade filtration according to claim 1 and 2 and nucleic acid purification device, it is characterized in that, described nucleic acid adsorption column is provided with internal thread, and the clamping mouth that has been threaded below described nucleic acid adsorption column, is provided with nucleic acid adsorption film in described clamping mouth.
4. biological specimen cascade filtration according to claim 3 and nucleic acid purification device, is characterized in that, described nucleic acid adsorption film is provided with compacting ring, and described clamping gonys portion is provided with discharge port.
5. biological specimen cascade filtration according to claim 4 and nucleic acid purification device, is characterized in that, described Filter column comprises the filtering membrane that shaft and shaft bottom arrange, and described filtering membrane is provided with compacting ring, and described Filter column bottom is provided with discharge port.
6. biological specimen cascade filtration according to claim 3 and nucleic acid purification device, is characterized in that, described clamping mouth is provided with for the clamping shirt rim with the clamping of centrifuge tube inwall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420316267.7U CN203999608U (en) | 2014-06-16 | 2014-06-16 | A kind of biological specimen cascade filtration and nucleic acid purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420316267.7U CN203999608U (en) | 2014-06-16 | 2014-06-16 | A kind of biological specimen cascade filtration and nucleic acid purification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203999608U true CN203999608U (en) | 2014-12-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420316267.7U Expired - Lifetime CN203999608U (en) | 2014-06-16 | 2014-06-16 | A kind of biological specimen cascade filtration and nucleic acid purification device |
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| Country | Link |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779277A (en) * | 2015-01-13 | 2016-07-20 | 温和心 | Nucleic acid extraction suction tube |
| CN106086003A (en) * | 2016-06-17 | 2016-11-09 | 易初丽 | A kind of nucleic acid purification system |
| CN110628573A (en) * | 2019-11-11 | 2019-12-31 | 甘肃农业大学 | Potato nucleic acid extraction device and method |
| CN112375756A (en) * | 2020-11-12 | 2021-02-19 | 安徽森芃生物科技有限责任公司 | Method for fully-automatic nucleic acid extraction aiming at solid sample |
| CN113717837A (en) * | 2021-08-13 | 2021-11-30 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification post |
-
2014
- 2014-06-16 CN CN201420316267.7U patent/CN203999608U/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779277A (en) * | 2015-01-13 | 2016-07-20 | 温和心 | Nucleic acid extraction suction tube |
| CN106086003A (en) * | 2016-06-17 | 2016-11-09 | 易初丽 | A kind of nucleic acid purification system |
| CN110628573A (en) * | 2019-11-11 | 2019-12-31 | 甘肃农业大学 | Potato nucleic acid extraction device and method |
| CN110628573B (en) * | 2019-11-11 | 2023-04-21 | 甘肃农业大学 | Potato nucleic acid extraction device and method |
| CN112375756A (en) * | 2020-11-12 | 2021-02-19 | 安徽森芃生物科技有限责任公司 | Method for fully-automatic nucleic acid extraction aiming at solid sample |
| CN113717837A (en) * | 2021-08-13 | 2021-11-30 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification post |
| CN113717837B (en) * | 2021-08-13 | 2023-10-03 | 通用生物(安徽)股份有限公司 | Detachable medicinal nucleic acid purification column |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190626 Address after: Room 101, Building 2636 Yuhangtang Road, Cangqian Street, Yuhang District, Hangzhou City, Zhejiang Province Patentee after: Hangzhou Beiwo Medical Technology Co.,Ltd. Address before: U.S.A. Patentee before: Shi Jingyu |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141210 |