CN105779277A - Nucleic acid extraction suction tube - Google Patents
Nucleic acid extraction suction tube Download PDFInfo
- Publication number
- CN105779277A CN105779277A CN201510464228.0A CN201510464228A CN105779277A CN 105779277 A CN105779277 A CN 105779277A CN 201510464228 A CN201510464228 A CN 201510464228A CN 105779277 A CN105779277 A CN 105779277A
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- CN
- China
- Prior art keywords
- nucleic acid
- suction pipe
- acid extraction
- suction tube
- extraction suction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Abstract
The invention provides a nucleic acid extraction suction tube integrating the functions of filtration, adsorption, washing and elution. The nucleic acid extraction suction tube is characterized in that a suction tube, an annular rubber ring, a nucleic acid adsorption sheet, a long-nozzle small post and a protection cap are sequentially connected. Under the blowing and sucking effect of the suction tube, a nucleic acid sample and treatment liquid repeatedly go into and out of the nucleic acid absorption sheet through the long-nozzle small post, and the goals of extracting and purifying the nucleic acid from the sample by filtering impurities, adsorbing nucleic acid, washing and eluting the nucleic acid are achieved.
Description
Technical field
The present invention relates to one and extract nucleic acid device.
Background technology
Currently, molecular Biological Detection nearly all needs to extract sample nucleic, and nucleic acid extraction quality and efficiency decide the success or failure of testing result.Nucleic acid extraction technology chemically method develops into physisorphtion, although technical merit has bigger lifting, but being required for the equipment such as high speed centrifugation and repeatedly move liquid, the troublesome operation such as centrifugal, being unfavorable for grass-roots unit and field work, this is the primary obstacle that molecular Biological Detection technology is promoted in basic unit.
The chemical methodes such as tradition phenol-chloroform method extract nucleic acid, although the nucleic acid yield that the method is extracted is high, purity is good, effect is certain, but use the poisonous and harmful substance such as phenol and chloroform, operating process will repeatedly open pipe liquid feeding, be centrifuged, abandon liquid, frequently change centrifuge tube and nozzle, job step is tediously long numerous and diverse, causes extraction work efficiency low.Utilize the centrifugal column extraction method that the characteristic of SiO2 specific bond nucleic acid grows up, the method does not use the poisonous and harmful substance such as phenol and chloroform, nucleic acid yield and purity are also superior to chemical method, and the method is currently used main method for extracting nucleic acid, uses also the most extensive.Centrifugal column method extract nucleic acid exist bigger deficiency be centrifuge tube need repeatedly to uncap liquid feeding, be centrifuged, abandon liquid operation, when detection limit is big, cause very big operating pressure to operator, instrument.The nucleic acid extraction suction pipe of integration is to develop on post centrifugation technique basis, by highly integrated to the absorption of nucleic acid, washing and eluting function in a branched pipette, its principle is to embed in suction pipe by SiO2 material, sample is inhaled in suction pipe after lysate liquid effect, by SiO2 filtration of material in way, nucleic acid is wherein only had to be adsorbed securely, other impurity is blown and discards, then repeatedly inhale/blow cleaning mixture and remove other impurity in film and salt, treat that film is dried, sucking eluent by the Nucleic Acid Elution in film, the eluent obtained is nucleic acid extraction liquid.Extraction process does not need the miscellaneous equipments such as centrifuge, simple and convenient, and the Nucleic acid quality of extraction is suitable with centrifugal column method, greatly facilitates staff especially basic unit or the detection of field work personnel's nucleic acid extraction.
Summary of the invention
In view of this, it is an object of the invention to provide a kind of nucleic acid extraction suction pipe, the absorption of nucleic acid, washing and eluting function are integrated in a branched pipette by this nucleic acid extraction suction pipe, reduce the outfit of instrument and equipment, reagent storage and task complexity, thus improving nucleic acid extraction work efficiency and convenience.
For realizing reaching above-mentioned purpose, the technical solution used in the present invention is: the nucleic acid extraction suction pipe of integration, it is linked in sequence by suction pipe, ring apron, nucleic acid absorption sheet, long nozzle pillar, head protector, ring apron compresses nucleic acid absorption sheet, suction sheet is made to be close to long nozzle pillar bottom surface, suction pipe is locked long nozzle pillar cylinder sealing, and head protector is locked long nozzle pillar.Under suction pipe pressure-vaccum effect, nucleic acid samples and process liquid, through long nozzle pillar, pass in and out nucleic acid absorption sheet repeatedly, reach impurity screening, absorption nucleic acid, washing and eluting nucleic acid, reach the purpose of extraction, purification of nucleic acid from sample.Nucleic acid extraction suction pipe extracts nucleic acid and comprises the following specific steps that: S1: with cracking-in conjunction with liquid and sample blending, crack 10 minutes；S2: inhale/blow sample dissociation-in conjunction with liquid with nucleic acid extraction suction pipe, make liquid substantially pass nucleic acid absorption sheet, repeatedly inhale/blow 3 times, finally outside abundant for lysate discharge pipe；S3: nucleic acid extraction suction pipe is sucked cleaning mixture and is about 1mL, makes cleaning mixture through nucleic acid absorption sheet, blows and abandon, and repeats to suck/blow out cleaning mixture 2 times, is finally fully blown out by cleaning mixture, stand and make suction sheet dry in 5 minutes；S4: nucleic acid extraction suction pipe sucks eluent and is about 100uL, makes liquid fully infiltrate suction sheet, stands 2 minutes, by eluent blowout to another clean plastic tube, eluent is the nucleic acid of extraction purification.Above-mentioned cracking-in conjunction with liquid is high concentration guanidinesalt or other high level salt solution, and cleaning mixture is 70～100% alcohol water blends, and eluent is pure water or low salt buffer.Nucleic acid extraction suction pipe and auxiliary reagent store under normal temperature condition and transport, and are valid for one year.
The problem that instant invention overcomes existing nucleic acid extraction technical operation complexity and the equipment of dependence, only need in a nucleic acid extraction suction pipe when extracting nucleic acid, simple to operate quickly, do not need other instrument and equipment auxiliary, the Nucleic acid quality extracted is suitable with other extracting method, the requirement of conventional molecular Biological Detection can be met, thus significantly reducing nucleic acid extraction difficulty, solve a sample pre-treatments difficult problem in molecular Biological Detection work, especially solve grass-roots unit and a difficult problem for field sample nucleic acid extraction.
Accompanying drawing explanation
Fig. 1 is nucleic acid extraction suction pipe decomposing schematic representation；
In figure, nucleic acid extraction suction pipe resolves into five parts, is 1 respectively, inhales ball, and 2, ring apron, 3, nucleic acid absorption sheet, 4, long nozzle pillar, 5, head protector；
Fig. 2 is nucleic acid extraction suction pipe schematic appearance
Detailed description of the invention
Embodiment 1Shown in Fig. 1, the present invention includes the long nozzle pillar 4 of core part, is close to nucleic acid suction sheet 3 bottom long nozzle pillar 4, clamping nucleic acid absorption sheet 3 ring apron 2, inhales ball 1 by sealant long nozzle pillar 4 top that is locked, and be locked long nozzle pillar 4 lower portion cap 5.
Shown in Fig. 2, when using nucleic acid extraction suction pipe, take head protector 5, alternately suck-blowout sample dissociation liquid, cleaning mixture, eluent by clutching-loosen suction ball 1 action.
Embodiment 2Nucleic acid extraction suction pipe extracts solution example nucleic acid
1. respectively at nucleic acid extraction suction pipe wall record sample number into spectrum, take crack-be respectively placed in 2 centrifuge tubes standby in conjunction with liquid 500uL, eluent 100uL, cleaning mixture 3000mL is placed in 3 centrifuge tubes, 1000uL/ manage, standby.
2. take blood serum sample 100uL and cracking-fully mix, room temperature effect 10 minutes in conjunction with liquid；
3. sample dissociation-in conjunction with liquid is inhaled/blown to nucleic acid extraction suction pipe, makes liquid fully infiltrate suction sheet, repeatedly inhales/blow 3 times, finally outside abundant for lysate discharge pipe；
4. sucking/blow out 3 pipe cleaning mixture successively by previous step nucleic acid extraction suction pipe, make liquid fully infiltrate suction sheet, often pipe cleaning mixture repeats to suck/blow out 2 times, is finally fully blown out by cleaning mixture；
5. drying at room temperature 5 minutes；
6. previous step nucleic acid extraction suction pipe is sucked eluent and be about 100uL, make liquid fully infiltrate suction sheet, stand 2 minutes, by eluent blowout to another clean plastic tube；
7. in order to increase elution efficiency, previous step blowing out eluent and again sucks protokaryon acid extraction suction pipe, repeat step 6, eluent is the nucleic acid of extraction purification.
Embodiment 3Nucleic acid extraction suction pipe extracts suspension sample or solid sample nucleic acid
1. respectively at nucleic acid extraction suction pipe wall record sample number into spectrum, take crack-in conjunction with liquid 1000uL, add the mixing of 1uL mercaptoethanol standby.Cleaning mixture 6000uL is placed in 3 plastic tubes, and 2000uL/ manages, standby.Eluent 100uL is placed in 1 centrifuge tube standby；
2. suspension sample stands after taking 200uL and cracking-fully mix in conjunction with liquid, solid sample weigh 20mg and cracking-in conjunction with liquid be fully ground broken after stand, room temperature effect 20 minutes, make sample dissociation-fully precipitate in conjunction with liquid；
3. sample dissociation-in conjunction with liquid supernatant is inhaled/blown to nucleic acid extraction suction pipe, makes liquid fully infiltrate suction sheet, repeatedly inhales/blow 3 times, finally outside abundant for lysate discharge pipe；
4. sucking/blow out 3 pipe cleaning mixture successively by previous step nucleic acid extraction suction pipe, make liquid fully infiltrate suction sheet, often pipe cleaning mixture repeats to suck/blow out 2 times, is finally fully blown out by cleaning mixture；
5. drying at room temperature 5 minutes；
6. previous step nucleic acid extraction suction pipe is sucked eluent and be about 100uL, make liquid fully infiltrate suction sheet, stand 2 minutes, by eluent blowout to another clean plastic tube；
7. in order to increase elution efficiency, previous step blowing out eluent and again sucks protokaryon acid extraction suction pipe, repeat step 6, eluent is the nucleic acid of extraction purification.
Claims (3)
1. an integrated nucleic acid extraction suction pipe, is linked in sequence by suction pipe, ring apron, nucleic acid absorption sheet, long nozzle pillar, head protector, it is characterized in that: under suction pipe pressure-vaccum effect, and nucleic acid samples and process liquid, through long nozzle pillar, pass in and out nucleic acid absorption sheet repeatedly.
2. the nucleic acid extraction suction pipe of integration according to claim 1, is characterized in that: ring apron compresses nucleic acid absorption sheet, makes suction sheet be close to long nozzle pillar bottom surface.
3. the nucleic acid extraction suction pipe of integration according to claim 1, is characterized in that: suction pipe and long nozzle pillar cylinder are tightly connected, and during use, head protector can unload from nucleic acid extraction suction pipe.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2015200209322 | 2015-01-13 | ||
| CN201520020932 | 2015-01-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105779277A true CN105779277A (en) | 2016-07-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510464228.0A Pending CN105779277A (en) | 2015-01-13 | 2015-08-03 | Nucleic acid extraction suction tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105779277A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107574165A (en) * | 2017-09-27 | 2018-01-12 | 广州和实生物技术有限公司 | A kind of method for extracting nucleic acid and the extraction element for realizing this method |
| CN107815392A (en) * | 2017-11-10 | 2018-03-20 | 广州和实生物技术有限公司 | A kind of simple nucleic acid-extracting apparatus |
| CN109706056A (en) * | 2019-01-14 | 2019-05-03 | 北京君立康博科技有限公司 | Nucleic acid-extracting apparatus |
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| WO2000075623A1 (en) * | 1999-06-08 | 2000-12-14 | Dna Research Innovations Limited | Sample processing device |
| US20010018219A1 (en) * | 2000-02-28 | 2001-08-30 | Yoshiaki Igarashi | Method of recovering a plurality of nucleic acids by an identical stationary phase and an apparatus thereof |
| CN101684463A (en) * | 2008-09-28 | 2010-03-31 | 杭州优思达生物技术有限公司 | Method and kit for rapidly extracting nucleic acids from trace clinical samples |
| CN102472695A (en) * | 2009-07-09 | 2012-05-23 | 凸版印刷株式会社 | Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus |
| CN203663494U (en) * | 2014-01-14 | 2014-06-25 | 精专生医股份有限公司 | Sample extraction device, suction component thereof, fluid control device and accommodating suite |
| CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
| CN203999608U (en) * | 2014-06-16 | 2014-12-10 | 史经宇 | A kind of biological specimen cascade filtration and nucleic acid purification device |
-
2015
- 2015-08-03 CN CN201510464228.0A patent/CN105779277A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000075623A1 (en) * | 1999-06-08 | 2000-12-14 | Dna Research Innovations Limited | Sample processing device |
| US20010018219A1 (en) * | 2000-02-28 | 2001-08-30 | Yoshiaki Igarashi | Method of recovering a plurality of nucleic acids by an identical stationary phase and an apparatus thereof |
| CN101684463A (en) * | 2008-09-28 | 2010-03-31 | 杭州优思达生物技术有限公司 | Method and kit for rapidly extracting nucleic acids from trace clinical samples |
| CN102472695A (en) * | 2009-07-09 | 2012-05-23 | 凸版印刷株式会社 | Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus |
| CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
| CN203663494U (en) * | 2014-01-14 | 2014-06-25 | 精专生医股份有限公司 | Sample extraction device, suction component thereof, fluid control device and accommodating suite |
| CN203999608U (en) * | 2014-06-16 | 2014-12-10 | 史经宇 | A kind of biological specimen cascade filtration and nucleic acid purification device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107574165A (en) * | 2017-09-27 | 2018-01-12 | 广州和实生物技术有限公司 | A kind of method for extracting nucleic acid and the extraction element for realizing this method |
| CN107815392A (en) * | 2017-11-10 | 2018-03-20 | 广州和实生物技术有限公司 | A kind of simple nucleic acid-extracting apparatus |
| CN109706056A (en) * | 2019-01-14 | 2019-05-03 | 北京君立康博科技有限公司 | Nucleic acid-extracting apparatus |
| CN109706056B (en) * | 2019-01-14 | 2024-02-27 | 北京君立康科技发展有限责任公司 | Nucleic acid extraction device |
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Application publication date: 20160720 |