CN109706056A - Nucleic acid-extracting apparatus - Google Patents
Nucleic acid-extracting apparatus Download PDFInfo
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- CN109706056A CN109706056A CN201910152648.3A CN201910152648A CN109706056A CN 109706056 A CN109706056 A CN 109706056A CN 201910152648 A CN201910152648 A CN 201910152648A CN 109706056 A CN109706056 A CN 109706056A
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- nucleic acid
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- extracting apparatus
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- 102000039446 nucleic acids Human genes 0.000 claims abstract description 95
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 95
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 95
- 238000010521 absorption reaction Methods 0.000 claims abstract description 33
- 238000000605 extraction Methods 0.000 claims abstract description 27
- 238000004891 communication Methods 0.000 claims description 36
- 239000007788 liquid Substances 0.000 claims description 27
- 238000002156 mixing Methods 0.000 claims description 19
- 239000000463 material Substances 0.000 claims description 17
- 239000002245 particle Substances 0.000 claims description 16
- 230000005540 biological transmission Effects 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 14
- 238000009938 salting Methods 0.000 claims description 8
- 239000000919 ceramic Substances 0.000 claims description 4
- 238000005336 cracking Methods 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 229910021389 graphene Inorganic materials 0.000 claims description 3
- 229910052761 rare earth metal Inorganic materials 0.000 claims description 3
- 150000002910 rare earth metals Chemical class 0.000 claims description 3
- 229920001971 elastomer Polymers 0.000 claims description 2
- 239000000806 elastomer Substances 0.000 claims description 2
- 229910021426 porous silicon Inorganic materials 0.000 claims description 2
- 239000010802 sludge Substances 0.000 abstract description 6
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- 238000010586 diagram Methods 0.000 description 10
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- 238000001179 sorption measurement Methods 0.000 description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 8
- 230000008569 process Effects 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000006249 magnetic particle Substances 0.000 description 6
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
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- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 239000005373 porous glass Substances 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 235000009518 sodium iodide Nutrition 0.000 description 3
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- 238000010828 elution Methods 0.000 description 2
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 230000000640 hydroxylating effect Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
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- 239000000395 magnesium oxide Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- JKQOBWVOAYFWKG-UHFFFAOYSA-N molybdenum trioxide Chemical compound O=[Mo](=O)=O JKQOBWVOAYFWKG-UHFFFAOYSA-N 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 description 2
- 229910001950 potassium oxide Inorganic materials 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 2
- 229910001948 sodium oxide Inorganic materials 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
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- 239000002699 waste material Substances 0.000 description 2
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- BZSXEZOLBIJVQK-UHFFFAOYSA-N 2-methylsulfonylbenzoic acid Chemical compound CS(=O)(=O)C1=CC=CC=C1C(O)=O BZSXEZOLBIJVQK-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
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- 108010010803 Gelatin Proteins 0.000 description 1
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- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
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- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 1
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Abstract
The present invention relates to nucleic acid extraction technical fields, disclose a kind of nucleic acid-extracting apparatus, which includes: ontology, and the ontology has arrival end and the outlet end of inner cavity and the connection inner cavity；Filter device is equipped at the outlet end；Being full of in the inner cavity has porous nucleic acid absorption medium, and the diameter of the porous nucleic acid absorption medium is greater than the filter opening aperture of the filter device.A kind of nucleic acid-extracting apparatus provided by the invention, the nucleic acid absorption medium made of porous material carries out adsorbing and extracting to nucleic acid solution, porous media is capable of increasing absorption surface, improve nucleic acid extraction efficiency, the complex sample such as high-precision of excrement, sludge or soil is particularly suitable for detect, for the device of the invention without cooperating the complex devices such as centrifuge to use, structure is simple, convenient and efficient, is applicable in portable nucleic acid rapidly extracting on site simultaneously.
Description
Technical field
The present invention relates to nucleic acid extraction technical fields, more particularly to a kind of nucleic acid-extracting apparatus.
Background technique
The extracting method of usual nucleic acid is to carry out break process to the biological samples material such as cell, organization material first, is lost
Nuclease living discharges nucleic acid, and then except its hetero-organization or cell components such as deproteinized, polysaccharide, lipids, to obtain high quality
Nucleic acid.When extracting nucleic acid from complex samples such as excrement, sludge or soil, it is also necessary to be filtered before break process or pure
Change, to remove the particulate contamination in excrement, sludge or soil.However, being needed in the extraction process using centrifuge etc.
Complex device.
In recent years, with the appearance of solid phase adsorption technology and biochemical development, nucleic acid extraction is greatly facilitated.
However, being applicable in the bottleneck that portable nucleic acid rapid extracting method on site is still current nucleic acid extraction field.
Solid phase extraction techniques are mainly electrostatic, affine, ion exchange or the hydrogen bond etc. utilized between solid-phase adsorbent and nucleic acid
Interaction, to achieve the purpose that separate nucleic acid.Compared to traditional extracting method, solid phase extraction techniques have rapidly and efficiently
The advantages of, and organic phase and the shortcomings that being not completely separated from of water phase in liquid phase extraction can be overcome.Currently, solid phase extractions can divide
It is extracted for non magnetic solid phase extractions and Magneto separate.
Non magnetic solid phase extractions nucleic acid is mainly carried out in a manner of spin-column chromatography, by centrifugal action, to reach point
Purpose from absorption nucleic acid.Solid phase extractions process is generally divided into four cracking, combination, cleaning, elution steps, compared to traditional
Method, this method can greatly shorten the extraction time of nucleic acid.Nowadays numerous nucleic acid extraction kits are based on the method hair
Zhan Erlai.The disadvantage of this method is that, it is necessary to it carries out by centrifuge, when carrying out the operation of a large amount of samples, can not keep away
The generation for exempting from cross contamination, easily causes false positive results.
The magnetic-particle that Magneto separate extracts in nucleic acid for nucleic acid separation need to have superparamagnetism and surface functional groups
The two characteristics.Firstly, superparamagnetism guarantees that the aggregation and dispersion of magnetic-particle can be controlled by externally-applied magnetic field: secondly, magnetic
The functional groups of particle surface, have an effect with nucleic acid molecules under certain condition, enriched nucleic acid.Applied magnetic particle carries out
Nucleic acid extraction mainly includes three processes: one, nucleic acid molecules form magnetic-particle-nucleic acid complexes in conjunction with magnetic-particle；
Two, under the action of externally-applied magnetic field, separating magnetic particles-nucleic acid complexes；Three, Nucleic Acid Elution.Furthermore, it is necessary to the particle that is magnetic
In conjunction with nucleic acid molecules and isolated solution environmental.For example, Fe3O4 magnetic nanoparticle can be in the item of PEG-6000 and sodium chloride
DNA under part in enrichment of cell lysate.Magnetic-particle used at present include for example, the magnetic-particle of Silica-coated,
Carboxylated magnetic nanoparticle, the magnetic nanoparticle of gelatin coatings, magnetic nanoparticle of methacrylic acid modification etc., point
It not be used to extract DNA, RNA in corn, milk, bacterium or virus.This method technical costs is higher, related examination in the market
Agent box is expensive, although the nucleic acid extracted is purer, spininess is to micro-example, and the nucleic acid extracted is less, and
It is not suitable for clinical detection.
Currently, a variety of convenient and practical nucleic acid-extracting apparatus are disclosed in the prior art, but these devices or needs
It is to be improved with the cooperation of other complex devices (for example, centrifuge) or extraction efficiency.For the high efficiency extraction of nucleic acid, or spy
Be not complex sample high-precision detection etc. for be still insufficient.
Summary of the invention
(1) technical problems to be solved
Need to be complicated with the use of centrifuge etc. the purpose of the present invention is to solve nucleic acid-extracting apparatus in the prior art
Equipment and the not high technical problem of extraction efficiency, provide a kind of nucleic acid-extracting apparatus.
(2) technical solution
For the technical problems in the prior art, the present invention provides a kind of nucleic acid-extracting apparatus, which includes: this
Body, the ontology have arrival end and the outlet end of inner cavity and the connection inner cavity；Filter device is equipped at the outlet end；
Being full of in the inner cavity has porous nucleic acid absorption medium, and the diameter of the porous nucleic acid absorption medium is greater than the filter device
Filter opening aperture.
Further, the porous nucleic acid absorption medium be porous glass beads, rare earth material particle, graphene particles,
One of porous silicon, porous ceramic particles, porous glycan particle or a variety of mixtures.
Further, the salting liquid of auxiliary nucleic acid extraction cracking function is also preinstalled in the inner cavity of the ontology.
Further, the ontology is elastomer.
Further, further includes: be removably mounted on the inlet cover of the arrival end and be removably mounted on described
Outlet cap outside outlet end.
Further, further includes: agitating device；The agitating device includes: the mixing part being placed in the chamber body
Described external rotary driving part is arranged in part, and the driving force of the transmitting rotary driving part drives described stir
Mix the transmission parts of component rotation；The transmission parts are mounted in the mounting hole of the inlet cover, to connect the mixing part
Part and rotary driving part.
Further, the transmission parts include: driven shaft and bearing；The driven shaft is rotatably installed by bearing
In the mounting hole；One end of the driven shaft is connected with the drive shaft of the rotary driving part, and the other end is stirred with described
Component is mixed to be connected.
Further, the rotary driving part includes but is not limited to Minitype transmission motor, spring leaf or spring wire.
Further, the outlet cap includes: control valve, first outlet and second outlet, and the control valve includes making
First outlet and the first state of second outlet closing make first outlet is closed and second outlet is opened the second state and make
The third state that first outlet is opened and second outlet is closed.
Further, the outlet cap includes: valve opening, and the control valve is set to the valve opening；
Further, the control valve includes: the first communication port and the second communication port, and the control valve can be in the valve opening
Interior relative motion makes the control valve have the first state, second state and the third state, first shape
State is that first communication port moves to and opens the first outlet, and second communication port moves to and makes described second to go out
The position that mouth is closed；Second state moves to for first communication port closes the first outlet, and described second
Communication port moves to the position for opening the second outlet；The third state be first communication port move to make it is described
First outlet is closed, and second communication port moves to the position for closing the second outlet.
Further, the control valve further include: the first limiting section, the second limiting section, third limiting section, locating part and bullet
Property element；The locating part and elastic element are embedded at respectively in the outlet cap, and the locating part is arranged in the elasticity
The end of element and pushing the first limiting section, the second limiting section and third limiting section to by the elastic element makes the locating part exist
In the first state, second state, third state engaging corresponding with one of them.
(3) beneficial effect
A kind of nucleic acid-extracting apparatus provided in an embodiment of the present invention, nucleic acid absorption medium is to core made of porous material
Acid solution carries out adsorbing and extracting, and porous media is capable of increasing absorption surface, improves nucleic acid extraction efficiency, is particularly suitable for complicated sample
The product such as high-precision of excrement, sludge or soil detects, while the device of the invention is without cooperating the complex devices such as centrifuge to make
With structure is simple, convenient and efficient, is applicable in portable nucleic acid rapidly extracting on site.
Detailed description of the invention
Fig. 1 is the overall structure diagram of one embodiment of nucleic acid-extracting apparatus of the present invention；
Fig. 2 is the overall structure diagram of another embodiment of nucleic acid-extracting apparatus of the present invention.
Fig. 3 is the overall structure diagram of the another embodiment of nucleic acid-extracting apparatus of the present invention.
Fig. 4 is the structural schematic diagram for the embodiment that outlet cap of the present invention is in the third state；
Fig. 5 is the structural schematic diagram for the embodiment that outlet cap of the present invention is in first state；
Fig. 6 is the structural schematic diagram for the embodiment that outlet cap of the present invention is in the second state；
Fig. 7 is the structural schematic diagram of an embodiment of reception device of the present invention.
Wherein:
Ontology 1, arrival end 1a, outlet end 1b, filter device 2, inlet cover 3, outlet cap 4, agitating device 5, seal washer
6, inner cavity 11, control valve 41, first outlet 42, second outlet 43, valve opening 44, mixing component 51, rotary driving part 52, transmission
Component 53, the first communication port 411, the second communication port 412, the first limiting section 413, the second limiting section 414, third limiting section 415,
Locating part 416, elastic element 417, reception device 418.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiments of the present invention will be described in further detail.Following instance
For illustrating the present invention, but it is not intended to limit the scope of the invention.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood at this with concrete condition
Concrete meaning in invention.
Term " nucleic acid " of the invention refers to the inhereditary material of organism comprising DNA (DNA) and ribose
Two class of nucleic acid (RNA).Term " nucleic acid extraction " of the invention refers to the process of isolates and purifies nucleic acid from sample.Sample of the invention
Product had both included the complex mixture containing organism, also included the mixture containing nucleic acid substances.Wherein organism includes microorganism, example
The tissue or cell of such as bacterium, virus and animal individual (for example, mammal, the mankind)." mixture " herein had both wrapped
Include the mixture containing multi-solvents, also include the mixture of the ingredient containing many kinds of solids, further include simultaneously containing solvent and solid at
The mixture divided.The example of complex mixture containing organism includes excrement, sludge etc. containing multiple-microorganism, and nucleic acid mentions at this time
Take the process including separating nucleic acid from isolated organism in complex mixture and from organism.Mixture containing nucleic acid substances
Example include cell pyrolysis liquid, blood constituent (without cell) etc., nucleic acid extraction includes that nucleic acid is separated from mixture at this time
Substance, or further include the process that nucleic acid is separated from the combination of protein and nucleic acid.
Fig. 1 gives the structural schematic diagram of one embodiment of nucleic acid-extracting apparatus of the present invention；Fig. 2 gives nucleic acid of the present invention and mentions
Take the structural schematic diagram of another embodiment of device；Fig. 3 gives the structural representation of the another embodiment of nucleic acid-extracting apparatus of the present invention
Figure.As shown in Figs. 1-3.The nucleic acid-extracting apparatus includes: ontology 1, and the ontology 1 has inner cavity 11 and the connection inner cavity 11
Arrival end 1a and outlet end 1b；Filter device 2 is equipped at the outlet end 1b；Being full of in the inner cavity 11 has porous nucleic acid to inhale
Attached medium, the diameter of the porous nucleic acid absorption medium are greater than the filter opening aperture of the filter device 2.
Specifically, ontology 1 is the main part for nucleic acid extraction, is a kind of shell structure with inner cavity 11, shape
Including but not limited to cylindric (as shown in Figure 1), the funnel-form (as shown in Figure 2) of shape, round table-like (as shown in Figure 3) or multiple rule
The combination of shape, preferably funnel-form.Extruding and the revertible material after releasing stress, such as elasticity can be used in ontology 1
Material, such material can control volume or pressure change in internal cavities, be conducive to the discharge of liquid in internal cavities, from
And avoid using centrifuge or vacuumize or push rod plug control pressure change.
The arrival end 1a of ontology 1 is one end of shell, and outlet end 1b is one end opposite with arrival end 1a, preferably arrival end
1a is placed in the top of ontology 1, and outlet end 1b is placed in the bottom of ontology 1.The sealing cover for opening arrival end 1a, by nucleic acid to be extracted
Sample or sample solution are added in inner cavity 11 by arrival end 1a, and cover sealing cover to sealing state, and mentioned in the cavity
It takes, the waste liquid or nucleic acid extraction liquid after the completion of extracting are flowed out by outlet end 1b.Nucleic acid extraction process is in independent sealed ontology 1
It carries out, the various contamination phenomenons being likely to occur is effectively reduced.
Nucleic acid absorption medium is loaded in the inner cavity 11 of ontology 1, to the nucleic acid in adsorbing separation liquid to be extracted, nucleic acid absorption
No more than full in the 1/2 of entire inner cavity 11, quantity can be determined according to actual needs medium volume.In order to enhance the absorption of nucleic acid
Binding force improves the extraction efficiency of nucleic acid, and porous nucleic acid absorption medium, the particulate matter being made of porous materials, hole can be used
The preferred 1-20nm of diameter, porous media can increase adsorption surface area, and enhancing the binding force of nucleic acid, especially aperture is the hole 1-20nm
The relatively suitable nucleic acid of diameter enters the interior surface of porous media.Wherein, the diameter of porous media be influence nucleic acid absorption it is important because
Element, diameter should not be too large, excessive, and surface area becomes smaller, and influence adsorption capacity, also unsuitable too small, although surface area becomes larger,
Meeting blocking filtering device 2, influences to filter, and cannot be effectively separated with the particulate matter in sample, it is generally the case that porous
The diameter of medium is between 1-1000 microns, preferably 10-500 microns, 30-300 microns more preferable, 50-200 microns more preferable.
The porous nucleic acid absorption medium of the present embodiment includes porous glass beads, rare earth material particle, graphene particles, more
Any one porous adsorbing material or above two in the porous adsorbing materials such as hole silicon, porous ceramic particles, porous glycan particle
And the mixing porous material of two or more porous adsorbing materials, the outer surface of porous nucleic acid absorption medium have more hydroxyl or
Amino, for enhancing the adsorption function of nucleic acid.Diameter meet above-mentioned condition it is micron-sized under the conditions of, different porous nucleic acid are inhaled
Attached medium all has identical adsorption function to the absorption of nucleic acid.
The implementation case is illustrated by taking porous glass beads as an example, and ingredient includes silica, aluminium oxide, oxidation
Calcium, magnesia, sodium oxide molybdena and potassium oxide etc..The content of silica therein is 70-75wt%, and the content of aluminium oxide is 1-
2.5wt%, the content of calcium oxide are 8-10wt%, and the content of magnesia is 1.5-4.5wt%, the content of sodium oxide molybdena and potassium oxide
The sum of be 13-15wt%.The glass bead surface of mentioned component has the hydroxyl or amino of more content, is more advantageous to nucleic acid substances
Absorption.
It is further preferred that the surface of particulate matter of the invention is chemically modified, to increase the adsorbed hydroxyl content.Example
Such as, piranha solution can be used to be chemically modified hydroxylating to surface particles, the piranha solution is the dense sulphur of heat
Acid and dioxygen aqueous mixtures, for removing all organic matters of porous nucleic acid absorption dielectric surface, while can make most of material
Hydroxylating occurs for the surface of material.
It is possible to further pre-install salting liquid in the inner cavity of ontology 1 11.It is molten that salting liquid is known in the art salt salt
Liquid has the miscellaneous function of nucleic acid extraction cracking, including but not limited to sodium iodide, guanidinium isothiocyanate, sodium chloride and sodium chlorate
One of solution or a variety of combinations.From the aspect of adsorption effect, preferably salting liquid is sodium iodide and guanidinium isothiocyanate.
IodineSodium Solution and guanidinium isothiocyanate are very beneficial for the absorption of nucleic acid.In view of safety non-toxic, more preferable sodium iodide is molten
Liquid.Consider from economy point, preferably sodium chloride.The concentration of salting liquid is not especially limited, but the concentration of salting liquid should not mistake
Low, the adsorption effect of too low then nucleic acid is insufficient, also unsuitable excessively high, and the adsorption effect enhancing of excessively high then increased costs and nucleic acid is unknown
It is aobvious, usually in 2.5mol/L between 8mol/L, preferably 3-6mol/L, more preferable 3.5-5mol/L.The pH value of salting liquid is not yet
Preferably too low, too low, impurity especially protein and lipid material are easily deposited in nucleic acid absorption medium, are unfavorable for the suction of nucleic acid
It is attached, it is also unsuitable excessively high, it should not especially be higher than the PKa value of nucleic acid absorption medium, will lead to adsorption effect decline obviously, usual salt is molten
The pH value of liquid is 3-6, preferably 3.5-5, more preferably 4.
Filter device 2 is used for the particulate matter and liquid component being filtered to remove in liquid, may be provided at the outlet end 1b of ontology 1
Inside, close or abutting outlet end 1b, filter opening aperture are less than the diameter of nucleic acid absorption medium, nucleic acid absorption medium are retained in
In the inner cavity 11 of ontology 1.But the aperture of filter opening is also unsuitable too small, too small to be then easy to be blocked by the particle in liquid, usual filter opening
Aperture be greater than 1 micron, preferably greater than 5 microns, more preferably greater than 10 microns.Filter device 2 be preferably nylon wire, metal mesh,
The combination of one or more of sand core, wherein the material of sand core includes the combination of glass, ceramics or both.
A kind of nucleic acid-extracting apparatus provided in an embodiment of the present invention, nucleic acid absorption medium is to core made of porous material
Acid solution carries out adsorbing and extracting, and porous media is capable of increasing absorption surface, improves nucleic acid extraction efficiency, is particularly suitable for complicated sample
The product such as high-precision of excrement, sludge or soil detects, while the device of the invention is without cooperating the complex devices such as centrifuge to make
With structure is simple, convenient and efficient, is applicable in portable nucleic acid rapidly extracting on site.
On the basis of the above embodiments, in the present embodiment, nucleic acid-extracting apparatus of the invention further include: removably pacify
Inlet cover 3 mounted in the arrival end 1a and the outlet cap 4 that is removably mounted on outside the outlet end 1b.Specifically, entrance
Lid 3 and outlet cap 4 are made of, inlet cover 3 and arrival end 1a at lid-like capping and the annular side being fixed on capping
The lid of suitability closes, and the lid of outlet cap 4 and outlet end 1b suitability closes, and threaded connection can be used, the mode that snaps connection carries out
Detachable connection.Inlet cover 3 and outlet cap 4 are preferably made of hard material, preferably duroplasts, such as ABS.Inlet cover 3 and go out
Lid 4 can be sealed ontology 1, avoid influence of the nucleic acid extraction by environment, wherein can be inlet cover 3 and arrival end 1a's
Seal washer 6 is respectively set in junction and the junction of outlet cap 4 and outlet end 1b, is further sealed processing.
In the present embodiment, agitating device is equipped with by mounting hole in the inlet cover 3 of nucleic acid-extracting apparatus of the invention
5；The agitating device 5 includes: the mixing component 51 being placed in the chamber body 11, and the rotation being arranged in outside the ontology 1 is driven
Dynamic component 52, and transmit the transmission parts that the driving force of the rotary driving part 52 drives the mixing component 51 to rotate
53；The transmission parts 53 are mounted in the mounting hole of the inlet cover 3, to connect the mixing component 51 and rotary driving part
Part 52.
Wherein, transmission parts 53 can be specifically made of driven shaft and bearing, and driven shaft is rotatably mounted on by bearing
In the mounting hole of inlet cover 3, one end of driven shaft is connected with the drive shaft of rotary driving part 52, the other end and mixing component 51
It is connected.In addition, other drive mechanisms, such as elastic slice, clockwork spring etc. also can be used in transmission parts 53.
Rotary driving part 52 includes any one driving part of manual driving member, automatic driving part, including but
Be not limited to: swing handle, Minitype transmission motor, spring leaf, spring wire etc. have any one form of automatic rotation function
Driving part.Rotary driving part 52 moves mixing component 51 in the inner cavity of ontology 1 11, carries out to the liquid in inner cavity 11
Stirring promotes the absorption or desorption of nucleic acid and nucleic acid absorption medium.
Mixing component 51 is placed in the inner cavity 11 of ontology 1, and is moved in the inner cavity 11, and the flowing of liquid is promoted.
Mixing component 51 can be shown in Fig. 2 rodlike, and deviate from the central axis certain distance of 1 inner cavity 11 of ontology；Mixing component
51 can also be foliaceous shown in Fig. 3, and multiple blades are uniformly distributed about the central axis of the inner cavity 11 of ontology 1, are fixed on
The free terminal of mixing component 51 promotes liquid mixing or flowing.The width of blade is not more than the width of 1 internal cavities of ontology
1/2 or be the 1/2 of interior hollow cavity width.The length of mixing component 51 is suitable with the height of ontology 1, preferably ontology 1 height
70%-90%.
On the basis of the various embodiments described above, in the present embodiment, outlet cap 4 can select independent totally-enclosed outlet
Lid, or have the outlet cap of any one form of the outlet cap of control valve and liquid outlet function, wherein it is independent it is totally-enclosed go out
Lid has a sealing function in the present invention, and when use need to be auxiliary according to using step that the centrifuge tube for being equipped with respective volume is required to carry out
Nucleic acid extraction is helped, is preferably provided with the outlet cap (as shown in Figure 4) of control valve Yu liquid outlet function in the present embodiment.
Fig. 4 gives the structural schematic diagram of one embodiment of outlet cap, as shown in figure 4, the outlet cap 4 includes: control valve
41, first outlet 42 and second outlet 43, and the control valve 41 includes close first outlet 42 and second outlet 43 the
One state makes first outlet 42 is closed and second outlet 43 is opened the second state and go out the unlatching of first outlet 42 and second
The third state that mouth 43 is closed.
Specifically, control valve 41 can be made freely to convert between above-mentioned three kinds of states as needed, to control liquid
Outflow.The embodiment of the present invention can be convenient different fluid from different outlet streams by setting first outlet 42 and second outlet 43
Out, the pollution of sample is avoided.Wherein, first outlet 42 and second outlet 43 can be of similar shape, and can also be had different
Shape preferably has different shapes, distinguishes different outlets to facilitate.In certain embodiments, utility model of the present invention
First outlet 42 be waste liquid outlet, second outlet 43 be nucleic acid extraction liquid export.Refering to what is shown in Fig. 7, first outlet 42 and
Reception device 418 can be connected under two outlets 43.Reception device 418 can manage for 1.5mLEP, and reception device 418 passes through screw thread
It is connect with first outlet 42 or second outlet 43.
Further, the outlet cap 4 includes: valve opening 44, and the control valve 41 is set in the valve opening 44；The control
Valve 41 processed includes: the first communication port 411 and the second communication port 412, the control valve 41 can the relative motion in the valve opening 44,
It is described for so that the control valve 41 is had the first state, second state and the third state, the first state
First communication port 411, which moves to, opens the first outlet 42, and second communication port 412 moves to and makes described second to go out
The positions that mouth 43 is closed；Second state moves to for first communication port 411 closes the first outlet 42, and institute
It states the second communication port 412 and moves to the position for opening the second outlet 43；The third state is first communication port
411 move to and close the first outlet 42, and second communication port 412 moves to and closes the second outlet 43
Position.
Control valve 41 is arranged in valve opening 44, and control valve 41 can be moved axially along valve opening 44, leftward position, in
Between switch between position and right positions.Control valve 41 includes the first communication port 411 and the second communication port 412, above-mentioned first-class
Port 411 and the second communication port 412 can be with annular groove-shapeds, or through hole shape etc..
Control valve 41 is in the third state in Fig. 4, which moves to for the first communication port 411 makes first outlet 42
It closes, and the second communication port 412 moves to the position for closing second outlet 43.That is, the column part of control valve 41 hinders simultaneously
Every the situation of first outlet 42 and second outlet 43.
Control valve 41 is in first state in Fig. 5, as shown in figure 5, first state is that the first communication port 411 moves to and makes the
The position that one outlet 42 is opened, and the second communication port 412 moves to the position for closing second outlet 43；That is, when the first circulation
When mouth 411 is moved axially at first outlet 42, first outlet 42 is opened, and at the same time, the column part of control valve 41 is located at
At second outlet 43, second outlet 43 is closed.
Control valve 41 is in the second state in Fig. 6, as shown in fig. 6, the second state is that the first communication port 411 moves to and makes the
One outlet 42 is closed, and the second communication port 412 moves to the position for opening second outlet 43；That is, when the second communication port 412 is moved
When moving to second outlet 43, second outlet 43 is opened, and at the same time, the column part of control valve 41 is located at first outlet 42,
First outlet 42 is closed.
In addition, refering to what is shown in Fig. 4, the control valve 41 further include: the first limiting section 413, the second limiting section 414, third
Limiting section 415, locating part 416 and elastic element 417；The locating part 416 and elastic element 417 are embedded at the outlet respectively
In lid 4, and the locating part 416 is arranged in the end of the elastic element 417 and pushes the first limit to by the elastic element 417
Position portion 413, the second limiting section 414 and third limiting section 415 make the locating part 416 in the first state, described the
The engaging corresponding with one of them of two-state, the third state.Specifically, in the present embodiment, the first limiting section 413, second limit
Position portion 414 and third limiting section 415 are annular groove.Locating part 416 is preferably steel ball, and elastic element 417 is preferably helical spring.
As shown in figure 4, locating part 416 engages with the second centrally located limiting section 414, control valve 41 is in third
State.As shown in figure 5, locating part 416 engages with the third limiting section 415 for being located at right end, control valve 41 is in first state.
As shown in fig. 6, locating part 416 engages with the first limiting section 413 for being located at left end, control valve 41 is in the second state.It is axial
The switching between each state can be realized in push-and-pull control valve 41.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (11)
1. a kind of nucleic acid-extracting apparatus characterized by comprising ontology, the ontology have inner cavity and the connection inner cavity
Arrival end and outlet end；Filter device is equipped at the outlet end；Being full of in the inner cavity has porous nucleic acid absorption medium, institute
The diameter for stating porous nucleic acid absorption medium is greater than the filter opening aperture of the filter device.
2. nucleic acid-extracting apparatus according to claim 1, which is characterized in that the porous nucleic acid absorption medium is porous
One of glass microballon, rare earth material particle, graphene particles, porous silicon, porous ceramic particles, porous glycan particle are a variety of
Mixture.
3. nucleic acid-extracting apparatus according to claim 1, which is characterized in that be also preinstalled with auxiliary in the inner cavity of the ontology
The salting liquid of nucleic acid extraction cracking function.
4. nucleic acid-extracting apparatus according to claim 1, which is characterized in that the ontology is elastomer.
5. nucleic acid-extracting apparatus according to claim 1-4, which is characterized in that further include: it is removably installed
The arrival end inlet cover and be removably mounted on the outlet cap of the outlet end.
6. nucleic acid-extracting apparatus according to claim 5, which is characterized in that further include: agitating device；The agitating device
Include: the mixing component being placed in the chamber body, described external rotary driving part is set, and described in transmitting
The driving force of rotary driving part drives the transmission parts of the mixing component rotation；The transmission parts are mounted on the entrance
In the mounting hole of lid, to connect the mixing component and rotary driving part.
7. nucleic acid-extracting apparatus according to claim 6, which is characterized in that the transmission parts include: driven shaft and axis
It holds；The driven shaft is rotatably installed in the mounting hole by bearing；One end of the driven shaft and the rotation are driven
The drive shaft of dynamic component is connected, and the other end is connected with the mixing component.
8. nucleic acid-extracting apparatus according to claim 6, which is characterized in that the rotary driving part includes but is not limited to
Minitype transmission motor, spring leaf or spring wire.
9. nucleic acid-extracting apparatus according to claim 5, which is characterized in that the outlet cap includes: control valve, first goes out
Mouth and second outlet；And the control valve includes the first state for closing first outlet and second outlet, closes first outlet
It closes the second state opened with second outlet and makes the third state that first outlet is opened and second outlet is closed.
10. nucleic acid-extracting apparatus according to claim 9, which is characterized in that the outlet cap includes: valve opening, the control
Valve processed is set to the valve opening；
The control valve includes: the first communication port and the second communication port, the control valve can the relative motion in the valve opening, make
The control valve has the first state, second state and the third state, and the first state is described first
Communication port, which moves to, opens the first outlet, and second communication port moves to the position for closing the second outlet
It sets；Second state moves to for first communication port closes the first outlet, and second communication port moves
To the position for opening the second outlet；The third state moves to for first communication port closes the first outlet
It closes, and second communication port moves to the position for closing the second outlet.
11. nucleic acid-extracting apparatus according to claim 10, which is characterized in that the control valve further include: the first limit
Portion, the second limiting section, third limiting section, locating part and elastic element；The locating part and elastic element are embedded at described respectively
In outlet cap, and the locating part be arranged in the elastic element end and by the elastic element push to the first limiting section,
Second limiting section and third limiting section make the locating part in the first state, second state, the third shape
State engaging corresponding with one of them.
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