CN205590682U - D - amino acid oxidase extraction element for zymotic fluid - Google Patents
D - amino acid oxidase extraction element for zymotic fluid Download PDFInfo
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- CN205590682U CN205590682U CN201620393146.1U CN201620393146U CN205590682U CN 205590682 U CN205590682 U CN 205590682U CN 201620393146 U CN201620393146 U CN 201620393146U CN 205590682 U CN205590682 U CN 205590682U
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- centrifuge
- filter press
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- extraction element
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Abstract
The utility model provides a D the amino acid oxidase is the extraction element for the zymotic fluid, including flocculation and precipitation jar, a centrifuge, liquid storage pot I, filter press, the II, the 2nd centrifuge of liquid storage pot and immobilization jar, the feed inlet of flocculation and precipitation jar is linked together through the discharge gate of pipeline with the fermentation cylinder, and the discharge gate of flocculation and precipitation jar passes through pipe connection a to centrifuge, a centrifuge's mother liquor export is linked together with filter press's entry, and filter press's export feeds through to the 2nd centrifuge's feed inlet, and the 2nd centrifuge's mother liquor export is through pipeline intercommunication immobilization jar. The utility model discloses effectively detached the macromolecular species in the zymotic fluid, micro molecule impurity and some saline impurity, the separation precision is high, has improved active ingredient's concentration, equipment is compact, the device is simple common, and area is little, and it is with low costs to go into operation, the operability of device is good, low in labor strength, the batch processing volume is big, green.
Description
Technical field
This utility model relates to a kind of D-AAO fermentation liquid extraction element, belongs to technical field of chemical production equipment.
Background technology
It is pharmaceuticals industry development trend, the research to biological enzyme agent from now on that biological enzyme produces antibiotic, is premise and the basis of development enzyme process antibiotic.The industrialization of 7-ACA production biological enzyme agent is the key technology of development enzyme process antibiotic, is conducive to promoting China's pharmaceuticals industry in production technology industry, the raising of fermentation engineering level of industry.
D-AAO (DAAO) is a kind of exemplary protein enzyme with flavin adenine as prothetic group, catalytic reaction substrate is had to stereoisomerism selectivity and the broad spectrum activity of height, be widely used in the amino acid whose qualitative and quantitative analysis of D-, biosensor, l-amino acid and the production of 2-ketoacid；Its most important value is to be applied to two-step method to produce 7-amino-cephalosporanic acid (7-ACA).With cephalosporin as raw material, D-AAO oxidation cephalosporin generates Glularyl-7-amino-cephalo-alkanoic acid (GL-7-ACA), then deacylated tRNA generates 7-ACA under GL-7-ACA ACY is catalyzed.
The production process of D-AAO mainly includes seed culture, ferments, extracts and the several workshop section of immobilization.Fermentation is the basic operation in biological engineering, and sweat completes in aqueous, and target product in fermentation liquor content is the most less, and sweat can produce the materials such as substantial amounts of thalline, foreign protein and colloidal solid, thus results in productivity and the Quality Down of enzyme.Therefore, the extraction element of D-AAO fermentation liquid, determine the effective ingredient purity of final products, the stability of product quality, and then the production to follow-up 7-ACA and also have a certain impact.At present, conventional extraction element mostly is film filter, and the rate of filtration is slow, filter efficiency is low, and the service life of filter membrane is short, and production cost is high.
Utility model content
The purpose of this utility model is to provide a kind of D-AAO fermentation liquid extraction element, it is achieved the rapidly and efficiently filtration to D-AAO fermentation liquid.
For achieving the above object, this utility model adopts the following technical scheme that
A kind of D-AAO fermentation liquid extraction element, including flocculation sediment tank, the first centrifuge, fluid reservoir I, filter press, fluid reservoir the II, second centrifuge and immobilization tank；The charging aperture of described flocculation sediment tank is connected with the discharging opening of fermentation tank by pipeline, and the discharging opening of flocculation sediment tank is connected to the first centrifuge by pipeline；The mother liquor outlet of the first centrifuge is connected with the entrance of filter press, the charging aperture of the outlet of filter press to the second centrifuge, and the mother liquor outlet of the second centrifuge passes through pipeline communication immobilization tank；
The fluid reservoir I of temporary mother solution it is provided between the mother liquor outlet and the entrance of filter press of the first centrifuge；The fluid reservoir II for keeping in mother solution it is also equipped with between the outlet and the entrance of the second centrifuge of filter press.
Further improvement of the utility model is: the flocculant entrance of the filtered solid outlet connection flocculation sediment tank of described first centrifuge.
Further improvement of the utility model is: be respectively arranged with dnockout pump A, dnockout pump B, dnockout pump C, dnockout pump D between described fermentation tank and flocculation sediment tank, between flocculation sediment tank and the first centrifuge, between fluid reservoir I and filter press, between fluid reservoir II and the second centrifuge.
Further improvement of the utility model is: described first centrifuge is butterfly centrifugal machine, and its separation is equivalent to 1000 mesh filter cloth specifications, and centrifugal rotational speed is 7500rpm.
Further improvement of the utility model is: the filter cloth specification of described filter press is 400 mesh, and filter pressure is 0.1 ± 0.05MPa, and filter pressing temperature is 10 ± 2 DEG C.
Further improvement of the utility model is: described second centrifuge is tube centrifuge, and its separation is equivalent to 1500 mesh filter cloth specifications, and centrifugal rotational speed is 10000rpm.
The beneficial effects of the utility model are:
This utility model provides a kind of D-AAO fermentation liquid extraction element, by the flocculation sediment tank being sequentially connected with, the first centrifuge, filter press and the setting of the second centrifuge, effectively eliminate the macromolecular substances in fermentation liquid, small molecular weight impurity and some salt impurity, separation accuracy is high, improve the concentration of effective ingredient, make the impurities in liquid content content the lowest, D-AAO entering immobilization tank the highest, it is ensured that the purity of D-AAO product and quality.
This utility model facility compact, device are the most common, and floor space is little, and investment cost is low；The good operability of device, labor intensity is low.Process batch of the present utility model is big, it is possible to meet quantity-produced batch requirement；And do not introduce other impurity and fresh water, and the repeatable utilization of flocculant, environmental protection, it is suitable for industrialization promotion.
The difference of the molecular size filtered as required, this utility model selects different size and the equipment of model.The fermentation liquid impurity particle adding flocculant is very big, and the butterfly centrifugal machine using separation to be equivalent to 1000 mesh filter cloth specifications is centrifuged, and centrifugal speed is fast, and separating degree is good；Then the filter press using filter cloth specification to be 400 mesh carries out high-rate fitration under filter pressing, then the pressure effect being suitable for and falls to have the impurity of larger particles；The tube centrifuge finally using separation to be equivalent to 1500 mesh filter cloth specifications is centrifuged, in order to remove the impurity of smaller particle.So setting of classification remove impurity, on the one hand plays a good protection to centrifuge and filter press, in the service life of extension device, on the other hand can alleviate the loss of equipment, dramatically speed up the rate of filtration.
Fluid reservoir I, fluid reservoir II it is respectively provided with, the fermentation liquid after temporary separation, it is to avoid rear stage equipment is caused damage by the pressure of previous stage equipment after first centrifuge of the present utility model, filter press.
Accompanying drawing explanation
Fig. 1 is integrated connection schematic diagram of the present utility model；
In figure, the direction of arrow is the flow direction of fermentation liquid.
In figure, each list of reference numerals is: 1, fermentation tank, 2, flocculation sediment tank, and the 3, first centrifuge, 4, fluid reservoir I, 5, filter press, 6, fluid reservoir the II, 7, second centrifuge, 8, immobilization tank, 9, dnockout pump A, 10, dnockout pump B, 11, dnockout pump C, 12, dnockout pump D.
Detailed description of the invention
Below in conjunction with the accompanying drawings this utility model is described further.
A kind of D-AAO fermentation liquid extraction element, as it is shown in figure 1, include flocculation sediment tank the 2, first centrifuge 3, fluid reservoir I 4, filter press 5, fluid reservoir the II 6, second centrifuge 7 and immobilization tank 8.The main device extracting separation dependence of D-AAO fermentation liquid includes flocculation sediment tank the 2, first centrifuge 3, filter press the 5, second centrifuge 7, and these device sequences arrange, by effective removal of impurity various in fermentation liquid.
The charging aperture of flocculation sediment tank 2 is connected by the discharging opening of pipeline with fermentation tank 1, and the discharging opening of flocculation sediment tank 2 is connected to the first centrifuge 3 by pipeline.The effect of described flocculation sediment tank 2 is the addition by flocculant, the partial impurities in absorption fermentation liquid, and beneficially later separation.
First centrifuge 3 is for carrying out initial gross separation by the fermentation liquid after flocculation, and its separation is equivalent to 1000 mesh filter cloth specifications.Filter cake obtained by centrifuge dripping still has flocculation, is sent back to the flocculant entrance of flocculation sediment tank 2 by the filtered solid outlet of the first centrifuge 3, it is achieved the recycling of flocculant.The liquid outlet of the first centrifuge is connected with the fluid reservoir I being used for keeping in first separation liquid.
The outlet of fluid reservoir I is connected by the entrance of pipeline with filter press 5, and the fermentation liquid after initial gross separation is admitted to filter press and carries out secondary separation.Filter press 5 can remove some large granular impurities in fermentation liquid, and its filter cloth specification is 400 mesh, and filter pressure is 0.1MPa, and filter pressing temperature is 10 DEG C..The outlet of filter press 5 is connected with the fluid reservoir II being used for keeping in secondary separation liquid.
The outlet of fluid reservoir II charging aperture by pipeline communication to the second centrifuge 7, the fermentation liquid after secondary separation is admitted to the second centrifuge 7 and carries out three separation.Second centrifuge 7 is for removing the little granule foreign of some in fermentation liquid, and its separation is equivalent to 1500 mesh filter cloth specifications..The liquid outlet of the second centrifuge 7 passes through pipeline communication immobilization tank 8, it is achieved enzyme immobilizatio reacts.
The mobilization dynamic of fermentation liquid is provided by dnockout pump, and this utility model arranges four dnockout pumps altogether, and wherein, dnockout pump A9 is arranged between fermentation tank 1 and flocculation sediment tank 2；Dnockout pump B10 is arranged between flocculation sediment tank 2 and the first centrifuge 3；Dnockout pump C11 is arranged between fluid reservoir I 4 and filter press 5；Dnockout pump D12 is arranged between fluid reservoir II 6 and the second centrifuge 7.
Use process of the present utility model is:
1, fermentation liquid is flowed out by fermentation tank, sends into flocculation sediment tank under the effect of dnockout pump A, adds flocculant in fermentation liquid, and after a period of time stirs, a part of impurity is adsorbed or sticks on flocculant；
2, the fermentation liquid in flocculation sediment tank is sent under the effect of dnockout pump B the first centrifuge 3, be centrifuged separating；Centrifugal filter cake is sent flocculation sediment tank back to and is done flocculant recycling, and centrifuge mother liquor gets rid of keeps in into fluid reservoir I；
3, the liquid in fluid reservoir I carries out filter pressing separation through dnockout pump C feeding filter press, filters the impurity that granule is bigger, and gained filtrate is sent into fluid reservoir II and kept in；
4, the liquid in fluid reservoir II is sent into the second centrifuge through dnockout pump D and is carried out secondary centrifuging separation, removes the impurity that granule is less；Extracting through three times and separate, in remaining liq, the purity of D-AAO is the highest, is sent directly into immobilization tank and carries out enzyme immobilization.
The foregoing is only preferred embodiment of the present utility model, all impartial changes done according to this utility model claim and modification, all should belong to covering scope of the present utility model.
Claims (5)
1. a D-AAO fermentation liquid extraction element, it is characterised in that: include flocculation sediment tank (2), the first centrifuge (3), fluid reservoir I (4), filter press (5), fluid reservoir II (6), the second centrifuge (7) and immobilization tank (8)；The charging aperture of described flocculation sediment tank (2) is connected by the discharging opening of pipeline with fermentation tank (1), and the discharging opening of flocculation sediment tank (2) is connected to the first centrifuge (3) by pipeline；The mother liquor outlet of the first centrifuge (3) is connected with the entrance of filter press (5), the outlet of filter press (5) passes through pipeline communication immobilization tank (8) to the charging aperture of the second centrifuge (7), the mother liquor outlet of the second centrifuge (7)；
The fluid reservoir I (4) of temporary mother solution it is provided between the mother liquor outlet and the entrance of filter press (5) of the first centrifuge (3)；The fluid reservoir II (6) for keeping in mother solution it is provided with between the outlet and the entrance of the second centrifuge (7) of filter press (5).
A kind of D-AAO fermentation liquid extraction element the most according to claim 1, it is characterised in that: the flocculant entrance of filtered solid outlet connection flocculation sediment tank (2) of described first centrifuge (3).
A kind of D-AAO fermentation liquid extraction element the most according to claim 1, it is characterised in that: it is respectively arranged with dnockout pump A (9), dnockout pump B (10), dnockout pump C (11), dnockout pump D (12) between described fermentation tank (1) and flocculation sediment tank (2), between flocculation sediment tank (2) and the first centrifuge (3), between fluid reservoir I (4) and filter press (5), between fluid reservoir II (6) and the second centrifuge (7).
A kind of D-AAO fermentation liquid extraction element the most according to claim 1, it is characterised in that: described first centrifuge (3) is butterfly centrifugal machine, and the second centrifuge (7) is tube centrifuge.
A kind of D-AAO fermentation liquid extraction element the most according to claim 1, it is characterised in that: the filter cloth of described filter press (5) is 400 mesh filter clothes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620393146.1U CN205590682U (en) | 2016-05-04 | 2016-05-04 | D - amino acid oxidase extraction element for zymotic fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620393146.1U CN205590682U (en) | 2016-05-04 | 2016-05-04 | D - amino acid oxidase extraction element for zymotic fluid |
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| Publication Number | Publication Date |
|---|---|
| CN205590682U true CN205590682U (en) | 2016-09-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620393146.1U Expired - Fee Related CN205590682U (en) | 2016-05-04 | 2016-05-04 | D - amino acid oxidase extraction element for zymotic fluid |
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| Country | Link |
|---|---|
| CN (1) | CN205590682U (en) |
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2016
- 2016-05-04 CN CN201620393146.1U patent/CN205590682U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160921 |
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| CF01 | Termination of patent right due to non-payment of annual fee |