CN102603814B - Method for increasing crystalizing efficiency of xylose in xylose mother solution - Google Patents
Method for increasing crystalizing efficiency of xylose in xylose mother solution Download PDFInfo
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- CN102603814B CN102603814B CN201210056432.5A CN201210056432A CN102603814B CN 102603814 B CN102603814 B CN 102603814B CN 201210056432 A CN201210056432 A CN 201210056432A CN 102603814 B CN102603814 B CN 102603814B
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Abstract
The invention belongs to the fields of biology and chemical industry, and particularly discloses a method for increasing the crystalizing efficiency of xylose in a xylose mother solution. The method comprises the following steps of: diluting the xylose mother solution; adding nutritional components; sterilizing; inoculating yeast for fermenting; removing glucose and galactose from the xylose mother solution; centrifugally separating yeast out of a zymotic fluid; filtering a clear zymotic fluid with a cross flow tube film; and removing a colloid contained in the zymotic fluid and fermentation metabolic products such as free proteins and the like produced in a fermenting process to obtain an ultra-filtered clear solution containing xylose and arabinose for use in subsequent production. The method has the advantages of simple steps, low treatment cost, high separating accuracy, good separating effect, great reduction in the subsequent treatment difficulty, great increase in product yield and suitability for wide popularization and application.
Description
(1) technical field
The invention belongs to biology and chemical field, particularly a kind of method improving xylose crystalline efficiency in xylose mother liquid.
(2) background technology
Xylose product mainly with corn cob, bagasse etc. for raw material, through the white crystals body that the operations such as hydrolysis, purification, evaporation, crystallization, centrifugal, oven dry are made, in light industry, food, chemical industry, tool has been widely used.Xylose mother liquid concentration is about 60%, and wherein wood sugar 40 ~ 50%, in addition, glucose 15 ~ 20%, pectinose 15 ~ 30%, semi-lactosi 8 ~ 12%, due to the existence of these components, xylose mother liquid cannot crystallization, can only sell as byproduct.
Although there is report to utilize simulation moving-bed purifying xylose mother liquor to produce the technique (patent No. 200710014175.8) of wood sugar and pectinose at present; and large-scale production; but the pre-treatment of xylose mother liquid is not thorough; the thalline of the meta-bolites that the semi-lactosi wherein existed, colloid, albumen and yeast fermentation produce and fragmentation can not thoroughly remove, and brings very large difficulty to decolorization filtering.Because traditional vacuum drum filters and Plate Filtration technique, the direction of liquid-flow is consistent with the direction of filtration, in liquid, the impurity such as colloid is attached directly on filtration medium very soon, by plugged filtration media, the materials such as colloid contained in feed liquid and albumen can not be removed, fermented liquid after general Plate Filtration decolouring, on average the filtration yield of every square metre of filter cloth is at 2 ~ 3m
3, have to afterwards change filter cloth, clean; In addition, also need in vacuum drum filtration procedure to consume the flocculating aidss such as a large amount of diatomite, add the running cost of filtration on the one hand, flocculating aids can take away partial material on the other hand, adds the loss of material; Finally, because the HPLC chromatographic peak distance of wood sugar and semi-lactosi is very near, utilize simulation moving-bed parting liquid semi-lactosi can not be gone out from wood sugar component, semi-lactosi major part is caused all to be present in wood sugar component, the purity of wood sugar is significantly reduced, and the existence of semi-lactosi makes the yield of xylose crystalline about 30%.
(3) summary of the invention
The present invention, in order to make up the deficiencies in the prior art, provides the method for xylose crystalline efficiency in the raising xylose mother liquid that a kind of step is simple, separation accuracy is high.
The present invention is achieved through the following technical solutions:
Improve a method for xylose crystalline efficiency in xylose mother liquid, be raw material with xylose mother liquid, mainly comprise the steps:
(1) nitrogen nutrition accounting for diluent weight 0.1 ~ 1% is added after xylose mother liquid being diluted to the concentration of 15 ~ 25%, then the pH value of diluent is regulated to be 4.0 ~ 4.5, sterilizing 30 minutes at 115 ~ 120 DEG C, barms is accessed after being cooled to 24 ~ 26 DEG C, inoculum size is often liter of diluent 12g dry yeast, then ferments at 20 ~ 40 DEG C;
(2) fermented liquid is centrifugal, centrifugal clear liquid returns fermentor tank, and dope is squeezed into yeast tank and stored;
(3) filtered at 35 ~ 80 DEG C through cross flow tube type film by centrifugal clear liquid, adopt membrane pore size to be the ultra-filtration membrane of 1000Da ~ 0.1 micron, ultrafiltration clear liquid is collected into ultrafiltration flow container, and ultrafiltration dope, again through centrifugal, reclaims feed liquid.
After xylose mother liquid dilutes by the present invention, add nutritive ingredient, sterilizing, then inoculation yeast is fermented, and goes out glucose in xylose mother liquid and semi-lactosi, after fermented liquid centrifugation goes out yeast, fermentation clear liquid is by cross-flow periosteum filtration treatment, remove the fermentating metabolism products such as the floating preteins produced in colloid contained in fermented liquid and fermenting process, finally obtain the ultrafiltration clear liquid containing wood sugar and pectinose, for subsequent production.
More excellent scheme of the present invention is:
In step (1), nitrogen nutrition is urea, ammonium chloride or ammonium sulfate, and its add-on is 0.3 ~ 0.5% of diluent weight.
In step (1), barms is one or more in bread yeast, cereuisiae fermentum or schizosaccharomyces pombe.
In step (1), during fermentation, air flow is 1:0.1 ~ 0.4, and stirring velocity is 50 ~ 100 turns/min, and fermentation time is 48h.
In step (2), the centrifugal employing disc centrifuge of fermented liquid.
In step (3), ultra-filtration membrane aperture is 100000Da, and operating temperature is 35 ~ 40 DEG C, filter pressure position 0.05 ~ 0.3MPa, and average flux is 60L/m
2h.
In step (3), described cross flow tube type membrane filtration comprises circulating filtration and continuous filtration, during continuous filtration, centrifugal clear liquid enters membrane filter plant, after a membrane filtration, before film, liquid continues to enter second group of ultra-filtration membrane, last to the 3rd group of ultra-filtration membrane, ultrafiltrated is unified is collected in ultrafiltration flow container, and before film, liquid is collected in centrifugal front tank, again through centrifugal, ultrafiltration after mixing next batch feed liquid, reach the object of maximum using feed liquid; During circulating filtration, centrifugal clear liquid enters membrane filter plant, and through certain cycling time, ultrafiltration clear liquid is collected in ultrafiltration flow container, and ultrafiltration dope is again through centrifugal, reclaims feed liquid.
The present invention is based on the basis of yeast fermentation, simulation moving-bed purifying xylose mother liquor, seek a kind of method that thoroughly can remove broken thalline and the meta-bolites produced in semi-lactosi in xylose mother liquid, colloid and Yeast fermentation process, obtain purer wood sugar and the mixed solution of pectinose.
The present invention utilizes cross flow tube type membrane filtration process, makes fermented liquid under a certain pressure by cross flow tube type film, collects filtered solution as lower operation raw material.Cross flow tube type filtration process, in vertical state, filtering direction is perpendicular to film surface, and liquid flow direction is then parallel to film surface for liquid flow direction and filtering direction, due to the quick flowing of liquid, make the impurity such as colloid can not rest on filter media surface, even if impurity is temporarily attached to filter media surface, quick flowing fluid can be washed away in time, so cross-flow filtration is not easy blocking filtering medium, the huge difference on molecular weight is there is due to impurity contained in xylose mother liquid, the broken thalline produced in colloid and Yeast fermentation process and meta-bolites belong to macromolecular substance, molecular weight is more than 100,000 or hundreds of thousands of, and wood sugar, the small molecular substance such as pectinose, molecular weight is about hundreds of, so utilize film device the macromolecular substance such as colloid can be retained, the compositions such as wood sugar then can through film, assemble in filtered solution one end, because the present invention adopts cross flow tube type membrane filtration, fermented liquid passes through at a high speed with tangential direction in film side, and the small molecules such as wood sugar pass through with the direction perpendicular to film, macromole is trapped within the fermented liquid side of film, due to the swiftly flowing souring of fermented liquid, make macromolecular substance can not at film superficial residence, be not easy to block fenestra, fermented liquid is after membrane filtration process, after activated carbon decolorizing, during Plate Filtration, on average the filtration yield of every square metre of filter cloth can reach 8 ~ 10m
3.
Step of the present invention is simple, and processing cost is low, and separation accuracy is high, good separating effect, significantly reduces subsequent disposal difficulty, can increase substantially product yield, be suitable for wide popularization and application.
(4) embodiment
Embodiment 1:
Get and be diluted to 20% xylose mother liquid 200ml, adjust pH4.3, add 1g urea, after sterilizing, be cooled to room temperature.Inoculation bread yeast 2.4g adds bread yeast, in 35 DEG C of shaking tables, ferments.Ferment after 24 hours, fermented liquid concentration is 17.4%, and the content measuring glucose is 0.23%, and galactose content 12.32%, after 48 hours, glucose content is 0, and galactose content is 1.72%.
Embodiment 2:
Get and be diluted to 20% xylose mother liquid 200ml, adjust pH4.7, add 1g ammonium chloride, after sterilizing, be cooled to room temperature.Inoculation bread yeast 2.4g adds bread yeast, in 35 DEG C of shaking tables, ferments.Ferment after 24 hours, fermented liquid concentration is 17.8%, and the content measuring glucose is 0, and galactose content 6.828%, after 48 hours, glucose content is 0, and galactose content is 1.587%.
Embodiment 3:
Xylose mother liquid raw material pump is squeezed into 30m
3in fermentor tank, adding process water, to be diluted to concentration be 21.5%, and diluent accounts for 2/3 of fermenter volume.Add potassium primary phosphate, ammonium sulfate and magnesium sulfate in 0.4% ratio, regulate pH4.4, in 115 ~ 120 DEG C of sterilizing 30min, after temperature is down to 35 DEG C, inoculation 360kg bread yeast, starts fermentation.Fermentation condition is: temperature 35 ~ 38 DEG C, pH4.0 ~ 4.5, stirring velocity 64 turns/min, ventilation 1:0.2.Concentration after about 9h of fermenting is 18.2%, and the content measuring glucose in fermented liquid is 0.37%, and galactose content is 12.54%.Ferment after 48 hours, glucose content is 0, and galactose content is 1.39%, terminates fermentation.Then fermented liquid being squeezed into disk plate centrifuge carries out centrifugal.After fermentation liquor disk plate centrifuge is centrifugal, fermentor tank returned by clear liquid, and dope is squeezed into yeast tank and stored.
Embodiment 4:
Fermentation liquor of xylose mother liquid 502kg, concentration is 17.8%, and temperature is 34 DEG C, filter pressure 0.1 ~ 0.3MPa.Membrane pore size used is 100,000Da, after film device, obtains filtrate 466.144kg, and concentration is 17%, transparent.Film average discharge is 1447g/min.Liquid 31.52kg before film, solid content is 4.6%, and thickness is opaque.
Embodiment 5:
Get membrane filtration liquid and fermentation centrifugal clear liquid 100mL respectively, concentration is 21.5%, adds 2g gac, in 55 ~ 60 DEG C of insulation 40min, uses filter paper filtering.Cut-off filtration time is 16min, and measure membrane filtration liquid and fermentation centrifugal clear liquid volume, wherein membrane filtration liquid 85 mL, fermentation centrifugal clear liquid 41 mL, filtration efficiency is for improving about one times.
Claims (5)
1. improve a method for xylose crystalline efficiency in xylose mother liquid, be raw material with xylose mother liquid, it is characterized by, mainly comprise the steps:
(1) nitrogen nutrition accounting for diluent weight 0.1 ~ 1% is added after xylose mother liquid being diluted to the concentration of 15 ~ 25%, then the pH value of diluent is regulated to be 4.0 ~ 4.5, sterilizing 30 minutes at 115 ~ 120 DEG C, barms is accessed after being cooled to 24 ~ 26 DEG C, inoculum size is often liter of diluent 12g dry yeast, then ferments at 20 ~ 40 DEG C; Barms is one or more in bread yeast, cereuisiae fermentum or schizosaccharomyces pombe;
(2) fermented liquid is centrifugal, centrifugal clear liquid returns fermentor tank, and dope is squeezed into yeast tank and stored;
(3) filtered at 35 ~ 80 DEG C through cross flow tube type film by centrifugal clear liquid, adopt membrane pore size to be the ultra-filtration membrane of 100000Da, operating temperature is 35 ~ 40 DEG C, and filter pressure is 0.05 ~ 0.3MPa, and average flux is 60L/m
2h, ultrafiltration clear liquid is collected into ultrafiltration flow container, and ultrafiltration dope, again through centrifugal, reclaims feed liquid.
2. the method for xylose crystalline efficiency in raising xylose mother liquid according to claim 1, it is characterized in that: in step (1), nitrogen nutrition is urea, ammonium chloride or ammonium sulfate, and its add-on is 0.3 ~ 0.5% of diluent weight.
3. the method for xylose crystalline efficiency in raising xylose mother liquid according to claim 1, it is characterized in that: in step (1), during fermentation, air flow is 1:0.1 ~ 0.4, and stirring velocity is 50 ~ 100 turns/min, and fermentation time is 48h.
4. the method for xylose crystalline efficiency in raising xylose mother liquid according to claim 1, is characterized in that: in step (2), the centrifugal employing disc centrifuge of fermented liquid.
5. the method for xylose crystalline efficiency in raising xylose mother liquid according to claim 1, it is characterized in that: in step (3), described cross flow tube type membrane filtration comprises circulating filtration and continuous filtration.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023116302A1 (en) | 2021-12-26 | 2023-06-29 | 浙江华康药业股份有限公司 | Method for co-producing erythritol and arabinose from xylose mother liquor |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103012506A (en) * | 2012-09-07 | 2013-04-03 | 山东绿健生物技术有限公司 | Energy-saving process for extracting crystallized xylose and arabinose from xylose mother liquor |
| CN106946691A (en) * | 2017-03-31 | 2017-07-14 | 山东福洋生物科技有限公司 | A kind of method for reclaiming fermented product in fermented product mother liquor |
| CN109880861A (en) * | 2019-03-06 | 2019-06-14 | 四川金象赛瑞化工股份有限公司 | A kind of method that xylose mother liquid continuously produces furfural |
| CN112480185B (en) * | 2020-11-09 | 2024-02-09 | 河南豫鑫糖醇有限公司 | Process for extracting xylose by biological method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643752A (en) * | 2009-06-26 | 2010-02-10 | 安徽丰原发酵技术工程研究有限公司 | Method for producing xylitol and L-arabinose by xylose mother liquor |
| CN101857523A (en) * | 2010-06-07 | 2010-10-13 | 禹城绿健生物技术有限公司 | Method for producing xylitol and arabitol simultaneously by utilizing xylose mother liquid |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643752A (en) * | 2009-06-26 | 2010-02-10 | 安徽丰原发酵技术工程研究有限公司 | Method for producing xylitol and L-arabinose by xylose mother liquor |
| CN101857523A (en) * | 2010-06-07 | 2010-10-13 | 禹城绿健生物技术有限公司 | Method for producing xylitol and arabitol simultaneously by utilizing xylose mother liquid |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023116302A1 (en) | 2021-12-26 | 2023-06-29 | 浙江华康药业股份有限公司 | Method for co-producing erythritol and arabinose from xylose mother liquor |
| US11866756B2 (en) | 2021-12-26 | 2024-01-09 | Zhejiang Huakang Pharmaceutical Co., Ltd. | Methods for co-producing erythritol and arabinose by using xylose mother liquor |
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Inventor after: Yu Li Inventor after: Xin Chengfu Inventor after: Jing Wenli Inventor after: Liu Hailiang Inventor before: Xin Chengfu Inventor before: Jing Wenli Inventor before: Liu Hailiang |
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