CN103130664A - Process method of extracting gamma-aminobutyric acid through membrane separation technique - Google Patents
Process method of extracting gamma-aminobutyric acid through membrane separation technique Download PDFInfo
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- CN103130664A CN103130664A CN 201110383174 CN201110383174A CN103130664A CN 103130664 A CN103130664 A CN 103130664A CN 201110383174 CN201110383174 CN 201110383174 CN 201110383174 A CN201110383174 A CN 201110383174A CN 103130664 A CN103130664 A CN 103130664A
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- aminobutyric acid
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Abstract
The invention discloses a process method of extracting gamma-aminobutyric acid through membrane separation technique. The process method of extracting the gamma-aminobutyric acid through the membrane separation technique effectively resolves the problems that separation and purification steps are complex, the cost is high, and the obtaining rate and the product purity are low and unstable when the gamma-aminobutyric acid is prepared in later period in a microbiological fermentation mode. According to the process method of extracting the gamma-aminobutyric acid through the membrane separation technique, steps of preprocessing, decoloration, microfiltration, nanofiltration, reverse osmosis concentration, later-period crystallization, washing, drying, and the like are carried out on fermentation liquor to obtain the gamma-aminobutyric acid with the high purity and the high recovery rate. The process method of extracting the gamma-aminobutyric acid through the membrane separation technique can keep the natural quality of the gamma-aminobutyric acid to the maximum extent, is characterized by being easy to grasp and popularize, low in production cost, high in the obtaining rate of product, high in purity, and the like, and has strong potential to be popularized in industrial production.
Description
Technical field
The present invention relates to a kind of processing method of extracting γ-aminobutyric acid with membrane separation technique, belong to biological technical field.
Background technology
γ-aminobutyric acid (GABA) has another name called aminobutyric acid, is a kind of nonprotein amino acid, is the product that L-glutamic acid is transformed by L-Glutamic decarboxylase.It is formed in the bodies of aminal and plant widely, and in plant, γ-aminobutyric acid is mainly as the anti-stress material; In Mammals, it is as main inhibitory neurotransmitter, extensively is present in central nervous system, peripheral nervous system and unifies in some endocrine cells, and the normal physiological function of animal body is played an important role.The physiological actions such as that γ-aminobutyric acid has is stable, anticonvulsion, hypotensive, hypermnesis have application widely in medicine and foodstuffs industry.
The preparation method of γ-aminobutyric acid mainly contains two kinds at present: microbe fermentation method and chemical synthesis.The Production by Microorganism Fermentation γ-aminobutyric acid is safe, pollution-free, and the γ-aminobutyric acid that China Ministry of Health obtains on September 27th, 2009 approval take Production by Microorganism Fermentation is as new resource food, but is only 20% to purity requirement.
Domestic extraction and separation process to natural fermented γ-aminobutyric acid is still immature at present, and extraction step is mainly: fermented liquid is removed mycelium (centrifugation, filtration), initial gross separation (absorption, ion-exchange, extraction), purifying (crystallization, recrystallization), dry packing.This processing step is more, equipment cost is higher, and the product yield and purity lower and unstable, run into certain difficulty in suitability for industrialized production.
Summary of the invention
The purpose of this invention is to provide a kind of processing method of γ-aminobutyric acid of extracting with membrane separation technique from fermented liquid.This processing method is mainly used the membrane separation plants such as microfiltration membrane, nanofiltration membrane, does not need to be implemented in the long equipment situation of too complicated, costliness and disengaging time, make its technological process concisely smooth and easy, technology condition be easy to grasp and control; And effectively improve yield and the purity that γ-aminobutyric acid is extracted, keep to greatest extent the natural sex of γ-aminobutyric acid, and strengthen the keeping quality of γ-aminobutyric acid.The present invention has reached the expection purpose.
Technique of the present invention comprises following key step:
(1) gamma-aminobutyric acid fermentation pre-treatment: fermented liquid is sterilized in fermentor tank (121 ℃ 30min), are cooled to normal temperature;
(2) γ-aminobutyric acid pretreatment fluid decolouring: step (1) treatment solution is added gac according to 2% addition, process 25min for 85 ℃, gac is processed according to ordinary method;
(3) micro-filtration: step (2) is processed the microfiltration membrane that the destainer that obtains flows through aperture 0.2 μ m, and the controlling diaphragm pipe pressure is between 0-0.4MPa, and flow velocity 60L/h, filtering particle diameter are greater than the impurity of 0.5 μ m, and the filtrate of collection is to non-conservation tank;
(4) nanofiltration: micro-filtration filtrate is flowed into by pipeline in the film pipe that the macromolecular nano filtering membrane below molecular weight cut-off 300 dalton is housed, the controlling diaphragm pipe pressure is between 0-1.5MPa, and flow velocity is 200L/h, collects effluent liquid to non-conservation tank;
(5) reverse osmosis concentration: after nanofiltration finishes, flow into the nanofiltration filtrate of collecting in the film pipe that the macromolecular nano filtering membrane below molecular weight cut-off 100 dalton is housed by pipeline, the controlling diaphragm pipe pressure is between 0-1.5MPa, flow velocity is 200L/h, the abandoned stream fluid, when the solution to be concentrated volume is concentrated into the 20L left and right, stop concentrating, get concentrated solution.
(6) crystallization of γ-aminobutyric acid concentrated solution, washing, drying: the concentrated solution of step (5) collection is added the ethanol of 3 times of volumes 95%, 4 ℃ of standing 16h filter and collect crystal; Add 95% ethanol in the 3ml/g ratio again, 45 ℃ are stirred 1h, filter and collect crystal; 100 ℃ of fluidized drying 1h of crystal with collecting obtain the γ-aminobutyric acid finished product.
Adopt the gamma-aminobutyric acid fermentation of above-mentioned art breading different batches, simplified separation purifying technique, improved product yield and purity.Total yield is 90% left and right, and the γ-aminobutyric acid purity that obtains is 99% left and right.Method has that easy grasp is easily promoted, the product yield and purity high and keep the characteristics such as natural quality, cost be low, have the potentiality of promoting in suitability for industrialized production.
The present invention is further illustrated below in conjunction with example.
Embodiment
Embodiment 1:
The main component of gamma-aminobutyric acid fermentation: γ-aminobutyric acid 168g/L, Pidolidone 0.28g/L transforms volume 350L.
Fermented liquid is warming up to 121 ℃ of sterilization 30min in fermentor tank, pass through fermentor tank chuck circulating water to normal temperature after sterilization finishes.treatment solution adds gac according to 2% addition, process 25min to decolour for 85 ℃, destainer is flow through the ceramic microfiltration membrane of aperture 0.2 μ m, the controlling diaphragm pipe pressure is between 0-0.4MPa, flow velocity 60L/h, the filtrate of collecting flows in the film pipe that the macromolecular nano filtering membrane below molecular weight cut-off 300 dalton is housed by pipeline, the controlling diaphragm pipe pressure is between 0-1.5MPa, flow velocity is 200L/h, collecting effluent liquid flows in the film pipe that the macromolecular nano filtering membrane below molecular weight cut-off 100 dalton is housed by pipeline, the controlling diaphragm pipe pressure is between 0-1.5MPa, flow velocity is 200L/h, the abandoned stream fluid, when the solution to be concentrated volume is concentrated into the 20L left and right, stop concentrating, get concentrated solution.The ethanol that adds 3 times of volumes 95% in the concentrated solution, 4 ℃ of standing 16h filter and collect crystal, then add 95% ethanol in the 3ml/g ratio, and 45 ℃ are stirred 1h, filter and collect crystal; 100 ℃ of fluidized drying 1h of crystal with collecting obtain the γ-aminobutyric acid finished product.The γ-aminobutyric acid total yield is 89.2%, and purity is 99.2%.
Embodiment 2:
The main component of gamma-aminobutyric acid fermentation: γ-aminobutyric acid 161g/L, Pidolidone 0.32g/L transforms volume 350L.
Fermented liquid is warming up to 121 ℃ of sterilization 30min in fermentor tank, pass through fermentor tank chuck circulating water to normal temperature after sterilization finishes.treatment solution adds gac according to 2% addition, process 25min to decolour for 85 ℃, destainer is flow through the ceramic microfiltration membrane of aperture 0.2 μ m, the controlling diaphragm pipe pressure is between 0-0.4MPa, flow velocity 60L/h, the filtrate of collecting flows in the film pipe that the macromolecular nano filtering membrane below molecular weight cut-off 300 dalton is housed by pipeline, the controlling diaphragm pipe pressure is between 0-1.5MPa, flow velocity is 200L/h, collecting effluent liquid flows in the film pipe that the macromolecular nano filtering membrane below molecular weight cut-off 100 dalton is housed by pipeline, the controlling diaphragm pipe pressure is between 0-1.5MPa, flow velocity is 200L/h, the abandoned stream fluid, when the solution to be concentrated volume is concentrated into the 20L left and right, stop concentrating, get concentrated solution.The ethanol that adds 3 times of volumes 95% in the concentrated solution, 4 ℃ of standing 16h filter and collect crystal, then add 95% ethanol in the 3ml/g ratio, and 45 ℃ are stirred 1h, filter and collect crystal; 100 ℃ of fluidized drying 1h of crystal with collecting obtain the γ-aminobutyric acid finished product.The γ-aminobutyric acid total yield is 89.8%, and purity is 99.4%.
Claims (5)
1. a membrane separation technique is extracted the processing method of γ-aminobutyric acid
(1) gamma-aminobutyric acid fermentation pre-treatment: fermented liquid is sterilized in fermentor tank (121 ℃ 30min), are cooled to normal temperature;
(2) γ-aminobutyric acid pretreatment fluid decolouring: step (1) treatment solution is added gac according to 2% addition, process 25min for 85 ℃, gac is processed according to ordinary method;
(3) micro-filtration: step (2) is processed the microfiltration membrane that the destainer that obtains flows through aperture 0.2 μ m, and the controlling diaphragm pipe pressure is between 0-0.4MPa, and flow velocity 60L/h, filtering particle diameter are greater than the impurity of 0.5 μ m, and the filtrate of collection is to non-conservation tank;
(4) nanofiltration: micro-filtration filtrate is flowed into by pipeline in the film pipe that the macromolecular nano filtering membrane below molecular weight cut-off 300 dalton is housed, the controlling diaphragm pipe pressure is between 0-1.5MPa, and flow velocity is 200L/h, collects effluent liquid to non-conservation tank;
(5) reverse osmosis concentration: after nanofiltration finishes, flow into the nanofiltration filtrate of collecting in the film pipe that the macromolecular nano filtering membrane below molecular weight cut-off 100 dalton is housed by pipeline, the controlling diaphragm pipe pressure is between 0-1.5MPa, flow velocity is 200L/h, the abandoned stream fluid, when the solution to be concentrated volume is concentrated into the 20L left and right, stop concentrating, get concentrated solution.
(6) crystallization of γ-aminobutyric acid concentrated solution, washing, drying: the concentrated solution of step (5) collection is added the ethanol of 3 times of volumes 95%, 4 ℃ of standing 16h filter and collect crystal; Add 95% ethanol in the 3ml/g ratio again, 45 ℃ are stirred 1h, filter and collect crystal; 100 ℃ of fluidized drying 1h of crystal with collecting obtain the γ-aminobutyric acid finished product.
2. membrane separation technique is extracted the processing method of γ-aminobutyric acid as described in claim 1, it is characterized in that, in step (3), the micro-filtration filter membrane is ceramic membrane, and the aperture is 0.2 μ m.
3. membrane separation technique is extracted the processing method of γ-aminobutyric acid as described in claim 1, it is characterized in that, in step (4), the nanofiltration filter membrane is aromatic polyamides class composite membrane, molecular weight cut-off 300 dalton.
4. membrane separation technique is extracted the processing method of γ-aminobutyric acid as described in claim 1, it is characterized in that, in step (5), reverse osmosis membrane is cellulose acetate membrane, and molecular weight cut-off is less than 100 dalton.
5. membrane separation technique is extracted the processing method of γ-aminobutyric acid as described in claim 1, it is characterized in that, in step (6), the γ-aminobutyric acid drying means is efficient fluidized drying, and drying temperature is 100 ℃, and be 1h time of drying.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498548A (en) * | 2014-11-27 | 2015-04-08 | 苏州嘉禧萝生物科技有限公司 | Production method for enriching gamma-aminobutyric acid from germinated brown rice |
| CN105461786A (en) * | 2016-01-23 | 2016-04-06 | 雅赛利(台州)制药有限公司 | Separation and purification process of vancomycin hydrochloride |
| CN107827766A (en) * | 2016-12-05 | 2018-03-23 | 南通励成生物工程有限公司 | A kind of gamma aminobutyric acid novel crystal forms and preparation method thereof |
| WO2018082096A1 (en) * | 2016-11-07 | 2018-05-11 | 南通励成生物工程有限公司 | NEW γ-PROPALANINE CRYSTAL FORM AND PREPARATION METHOD THEREFOR |
| CN108504562A (en) * | 2018-06-21 | 2018-09-07 | 江苏澳创生物科技有限公司 | A kind of system of production of L-threonine by fermentation and its application |
| CN112778150A (en) * | 2021-01-14 | 2021-05-11 | 华熙生物科技股份有限公司 | Novel crystal form of gamma-aminobutyric acid and preparation method thereof |
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2011
- 2011-11-28 CN CN 201110383174 patent/CN103130664A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498548A (en) * | 2014-11-27 | 2015-04-08 | 苏州嘉禧萝生物科技有限公司 | Production method for enriching gamma-aminobutyric acid from germinated brown rice |
| CN105461786A (en) * | 2016-01-23 | 2016-04-06 | 雅赛利(台州)制药有限公司 | Separation and purification process of vancomycin hydrochloride |
| CN105461786B (en) * | 2016-01-23 | 2018-11-16 | 雅赛利(台州)制药有限公司 | A kind of separation purifying technique of vancomycin hydrochloride |
| WO2018082096A1 (en) * | 2016-11-07 | 2018-05-11 | 南通励成生物工程有限公司 | NEW γ-PROPALANINE CRYSTAL FORM AND PREPARATION METHOD THEREFOR |
| US10604474B2 (en) | 2016-11-07 | 2020-03-31 | Nantong Licheng Biological Engineering Co., Ltd | Crystalline form of gamma-aminobutyric acid and preparation method thereof |
| CN107827766A (en) * | 2016-12-05 | 2018-03-23 | 南通励成生物工程有限公司 | A kind of gamma aminobutyric acid novel crystal forms and preparation method thereof |
| CN107827766B (en) * | 2016-12-05 | 2021-01-19 | 南通励成生物工程有限公司 | Gamma-aminobutyric acid crystal form and preparation method thereof |
| CN108504562A (en) * | 2018-06-21 | 2018-09-07 | 江苏澳创生物科技有限公司 | A kind of system of production of L-threonine by fermentation and its application |
| CN112778150A (en) * | 2021-01-14 | 2021-05-11 | 华熙生物科技股份有限公司 | Novel crystal form of gamma-aminobutyric acid and preparation method thereof |
| WO2022151995A1 (en) * | 2021-01-14 | 2022-07-21 | 华熙生物科技股份有限公司 | New crystal form of γ-aminobutyric acid and preparation method therefor |
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Application publication date: 20130605 |