CN102796779B - Biological method for preparing gamma-aminobutyric acid - Google Patents
Biological method for preparing gamma-aminobutyric acid Download PDFInfo
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- CN102796779B CN102796779B CN2012103045965A CN201210304596A CN102796779B CN 102796779 B CN102796779 B CN 102796779B CN 2012103045965 A CN2012103045965 A CN 2012103045965A CN 201210304596 A CN201210304596 A CN 201210304596A CN 102796779 B CN102796779 B CN 102796779B
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- Prior art keywords
- aminobutyric acid
- liquid
- clear liquid
- exchange resin
- gamma
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- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 title claims abstract description 183
- 229960003692 gamma aminobutyric acid Drugs 0.000 title claims abstract description 92
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 title claims abstract description 89
- 238000010170 biological method Methods 0.000 title abstract 3
- 239000007788 liquid Substances 0.000 claims abstract description 66
- 238000000034 method Methods 0.000 claims abstract description 28
- 238000002360 preparation method Methods 0.000 claims abstract description 18
- 241000894006 Bacteria Species 0.000 claims abstract description 16
- 239000013078 crystal Substances 0.000 claims abstract description 16
- 238000004519 manufacturing process Methods 0.000 claims abstract description 15
- 239000000843 powder Substances 0.000 claims abstract description 15
- 238000006243 chemical reaction Methods 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 12
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- 238000000909 electrodialysis Methods 0.000 claims abstract description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 6
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- 238000002425 crystallisation Methods 0.000 claims description 15
- 230000008025 crystallization Effects 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 14
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 13
- 239000004721 Polyphenylene oxide Substances 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 10
- 239000003456 ion exchange resin Substances 0.000 claims description 10
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 10
- 239000000758 substrate Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 9
- 210000001082 somatic cell Anatomy 0.000 claims description 9
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 7
- 239000000919 ceramic Substances 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 7
- 235000013922 glutamic acid Nutrition 0.000 claims description 7
- 239000004220 glutamic acid Substances 0.000 claims description 7
- 229910052708 sodium Inorganic materials 0.000 claims description 7
- 239000011734 sodium Substances 0.000 claims description 7
- QWCKQJZIFLGMSD-UHFFFAOYSA-N 2-Aminobutanoic acid Natural products CCC(N)C(O)=O QWCKQJZIFLGMSD-UHFFFAOYSA-N 0.000 claims description 6
- QWCKQJZIFLGMSD-GSVOUGTGSA-N D-alpha-aminobutyric acid Chemical compound CC[C@@H](N)C(O)=O QWCKQJZIFLGMSD-GSVOUGTGSA-N 0.000 claims description 6
- 230000002378 acidificating effect Effects 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000003729 cation exchange resin Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 239000011347 resin Substances 0.000 claims description 6
- 229920005989 resin Polymers 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 229920002774 Maltodextrin Polymers 0.000 claims description 5
- 239000005913 Maltodextrin Substances 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 5
- 229920002472 Starch Polymers 0.000 claims description 5
- 239000002518 antifoaming agent Substances 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 5
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 5
- 235000019800 disodium phosphate Nutrition 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 229940035034 maltodextrin Drugs 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 229920000570 polyether Polymers 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 239000001632 sodium acetate Substances 0.000 claims description 5
- 235000017281 sodium acetate Nutrition 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 4
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 229940073490 sodium glutamate Drugs 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 239000003957 anion exchange resin Substances 0.000 claims description 3
- 239000002585 base Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000013016 damping Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 16
- 235000014655 lactic acid Nutrition 0.000 abstract description 8
- 239000004310 lactic acid Substances 0.000 abstract description 5
- 238000003786 synthesis reaction Methods 0.000 abstract description 5
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- 239000012295 chemical reaction liquid Substances 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 11
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 8
- 229960002989 glutamic acid Drugs 0.000 description 6
- 239000013530 defoamer Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 229960000448 lactic acid Drugs 0.000 description 4
- 229960004249 sodium acetate Drugs 0.000 description 4
- 241000186660 Lactobacillus Species 0.000 description 3
- 244000299461 Theobroma cacao Species 0.000 description 3
- 230000031018 biological processes and functions Effects 0.000 description 3
- 229940039696 lactobacillus Drugs 0.000 description 3
- 241000186685 Lactobacillus hilgardii Species 0.000 description 2
- 229940024606 amino acid Drugs 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000003169 central nervous system Anatomy 0.000 description 2
- 235000019219 chocolate Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 1
- 241000186226 Corynebacterium glutamicum Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000009470 Theobroma cacao Nutrition 0.000 description 1
- 229940124277 aminobutyric acid Drugs 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000015173 baked goods and baking mixes Nutrition 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 235000019462 natural additive Nutrition 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- HNJBEVLQSNELDL-UHFFFAOYSA-N pyrrolidin-2-one Chemical compound O=C1CCCN1 HNJBEVLQSNELDL-UHFFFAOYSA-N 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
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CN2012103045965A CN102796779B (en) | 2012-08-24 | 2012-08-24 | Biological method for preparing gamma-aminobutyric acid |
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CN2012103045965A CN102796779B (en) | 2012-08-24 | 2012-08-24 | Biological method for preparing gamma-aminobutyric acid |
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Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102978250B (en) * | 2012-12-20 | 2014-07-30 | 江苏久吾高科技股份有限公司 | Method for producing Gamma-aminobutyric acid through centrifugal mother liquid of glutamic acid |
CN104561160A (en) * | 2014-12-22 | 2015-04-29 | 南通励成生物工程有限公司 | Method for preparing theanine by using biological method |
CN104531795A (en) * | 2015-01-13 | 2015-04-22 | 北京格力森生物工程技术有限公司 | Method for producing high-purity gamma-aminobutyric acid |
CN105671098A (en) * | 2016-04-06 | 2016-06-15 | 河南巨龙生物工程股份有限公司 | Method for producing L-2-aminobutyric acid by fermentation process |
CN106544372A (en) * | 2016-11-04 | 2017-03-29 | 广西多得乐生物科技有限公司 | A kind of method that gamma aminobutyric acid is purified from zymotic fluid |
CN106417480B (en) * | 2016-11-21 | 2019-11-08 | 昆明理工大学 | A kind of production technology of the bread rich in γ-aminobutyric acid |
CN106417533A (en) * | 2016-11-21 | 2017-02-22 | 昆明理工大学 | Making method of gamma-aminobutyric acid containing biscuits |
CN107827765A (en) * | 2016-12-05 | 2018-03-23 | 南通励成生物工程有限公司 | A kind of gamma aminobutyric acid hemi-hydrate crystalline and preparation method thereof |
CN107326052B (en) * | 2017-06-28 | 2020-12-22 | 岭南师范学院 | Method for improving glutamate decarboxylase activity by using D101 macroporous adsorption resin |
CN107252021A (en) * | 2017-07-10 | 2017-10-17 | 秦皇岛长胜营养健康科技有限公司 | Reduce asparagus drink of Intima-medial thickness effect and its preparation method and application |
CN108300742A (en) * | 2017-07-26 | 2018-07-20 | 南通励成生物工程有限公司 | A kind of method that current adding substrate enzyme prepares γ-aminobutyric acid |
CN109369431B (en) * | 2018-11-30 | 2021-06-18 | 沧州信联化工有限公司 | Crystallization method of gamma-aminobutyric acid |
CN109761832A (en) * | 2019-03-05 | 2019-05-17 | 内蒙古精晶生物科技有限公司 | A kind of purification process of 4-Aminobutanoicacid |
CN115322450B (en) * | 2022-08-25 | 2023-12-08 | 华熙生物科技股份有限公司 | Composition and preparation method and application thereof |
CN116655485B (en) * | 2023-06-14 | 2024-01-23 | 山东福瑞达生物科技有限公司 | Process for converting and extracting gamma-aminobutyric acid |
Family Cites Families (6)
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CN1276087C (en) * | 2005-03-07 | 2006-09-20 | 浙江大学 | Process for biologically synthesizing gamma-amino butyric acid |
CN101333548A (en) * | 2008-06-26 | 2008-12-31 | 南昌大学 | Method for preparing gamma-aminobutyric acid by lactobacillus brevis |
CN101838672B (en) * | 2010-05-10 | 2014-12-17 | 江南大学 | Method for producing gamma-amino butyric acid by using immobilized lactobacillus plantarum |
CN102108370B (en) * | 2010-12-02 | 2013-03-06 | 中国农业大学 | Semicontinuous fermentation method for producing gamma-aminobutyric acid by lactic acid bacteria |
CN102174449B (en) * | 2011-03-04 | 2012-08-22 | 天津科技大学 | Method for producing high-yield gamma-propalanine and application thereof |
CN102199636A (en) * | 2011-04-13 | 2011-09-28 | 王健 | Efficient preparation process of gamma-amino-n-butyric acid |
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