CN106544372A - A kind of method that gamma aminobutyric acid is purified from zymotic fluid - Google Patents
A kind of method that gamma aminobutyric acid is purified from zymotic fluid Download PDFInfo
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Abstract
The invention discloses a kind of method that gamma aminobutyric acid is isolated and purified from zymotic fluid, belongs to biological technical field.It includes the preparation and pretreatment of (1) zymotic fluid, (2) cation seperation column absorption, (3) dilute alkaline soln wash-out, (4) anion column decolourizes, (5) electrodialysis desalination, (6) concentration and drying and other steps, prepare solid-state gamma aminobutyric acid of the purity higher than 95%.The present invention have process is simple, energy consumption is low, production environment is good, without potential safety hazard the features such as, there is good facilitation to the industrialized production of gamma aminobutyric acid.
Description
Technical field
The present invention relates to biological technical field, more particularly to a kind of to separate from zymotic fluid, purify GABA
Method.
Background technology
GABA is a kind of animal nerve inhibitor, with hypotensive, treatment epilepsy, promotes sleep, reduces nerve
It is excited, strengthen the physiological actions such as memory, just because of this, GABA and its derivative have become the heat of medicament research and development
One of point.The acquisition of GABA at present has three kinds of approach：Plant extract, chemical synthesis and biofermentation.Plant extraction method
Due to GABA, in plant, content is relatively low, therefore there is a problem of extracting difficult, and chemical rule is swift in response, but
Have the disadvantage that energy consumption is big, high cost, and with certain potential safety hazard.Hence with Production by Microorganism Fermentation gamma-amino fourth
Acid is the Main way of current research.Lactic acid bacteria is a kind of microorganism of aliment security level, is had in the food such as Yoghourt, pickles
Extensively apply, there are some researches show that lactic acid bacteria can produce GABA using glutamic acid.
Title:A kind of extraction separation and purification method application of GABA, (patent) number:CN201410477049.6,
The applying date:2014.09.18.A kind of extraction separation and purification method of GABA is disclosed, is comprised the following steps：(1) will
Rice is smashed to pieces, adds ethanol water to be extracted, by extract centrifugation after extraction, take supernatant after smashing to pieces；(2) will be upper
Clear liquid is concentrated, after after concentration adjusted supernatant pH, by 732 cationic ion-exchange resins for activating；(3) again with ammoniacal liquor pair
732 cationic ion-exchange resins are eluted, and collect eluent, eluent is concentrated after wash-out, dense with ethanol purification again after concentration
Contracting thing, after purifying 3 times, obtains GABA.Independent claims item is：1. the extraction of a kind of GABA separates pure
Change method, it is characterised in that the extraction separation and purification method of described GABA is comprised the following steps：(1) rice is smash
It is broken, add ethanol water to be extracted after smashing to pieces, by extract centrifugation after extraction, take supernatant；(2) it is supernatant is dense
Contracting, after after concentration adjusted supernatant pH, by 732 cationic ion-exchange resins for activating；(3) again with ammoniacal liquor to 732 sun from
Sub-exchange resin is eluted, and collects eluent, eluent is concentrated after wash-out, uses ethanol purification concentrate after concentration again, pure
After changing 3 times, GABA is obtained.
Title:A kind of separation purifying technique Application Number (patent) of GABA:CN201010167007.4, application
Day:2010.05.10.A kind of separation purifying technique of GABA is disclosed, it efficiently solves bioanalysis and prepares γ-ammonia
Base butyric acid causes purification procedures lengthy and tedious as fermentation broth contents are complicated, industrializes the problem of high cost.The present invention passes through
Impurity in conversion fluid is reduced using biotransformation method, so as to simplify separating technology, then conversion fluid is simply decolourized, is obtained
To high-purity, the GABA of high-recovery.The inventive method is simple, and product recovery rate is high, is adapted to fairly large industry
Metaplasia is produced.Principal claim:A kind of separation purifying technique of gamma aminobutyric acid, the step of the technique includes following orders：(1) conversion method
Production gamma aminobutyric acid：Thalline culture is carried out first, by centrifugation or plate-frame filtering collects thalline, is suspended in acetate buffer solution
(pH 4.8), adds substrate L Glu, conversion reaction is carried out at 30 DEG C.(2) gamma aminobutyric acid conversion fluid pretreatment：Will conversion
Liquid is by centrifugation or is filtered to remove thalline, takes supernatant and heats 30min at 70-80 DEG C, first filtered with filter paper, then is filtered with 0.45 μm
Film carries out suction filtration, collects filtrate stand-by.(3) process of activated carbon：Activated carbon is processed according to conventional method, burgy → go from
(120 DEG C, 8h) → be cooled to room temperature is washed → filtered → be dried to sub- washing → hot deionized water, standby.(4) gamma aminobutyric acid is located in advance
Reason liquid decolourizes：The filtrate that step (2) is obtained is adjusted to pH5.0, active carbon powder is added according to 2% addition, at 60 DEG C
Lower insulated and stirred 30min, after double-layer filter paper is filtered, then carries out suction filtration with 0.45 μm of filter membrane, collects filtrate stand-by.(5) γ amino
Butyric acid destainer is concentrated in vacuo：The filtrate that step (4) is obtained is concentrated in vacuo to into thin viscous, heat preservation for standby use at 55 DEG C.(6) γ ammonia
Base butyric acid concentrate is crystallized：The concentrate of step (5) is added the 95% of 3 times of volumes ethanol, at 4 DEG C after static 12h, is filtered
Collect crystal.(7) washing of gamma aminobutyric acid crystal：The gamma aminobutyric acid crystal that step (6) is collected is brilliant according to 3 mls/g
The ratio of body adds 95% ethanol, 40 DEG C of stirring 1h that crystal is collected by filtration.(8) drying of gamma aminobutyric acid crystal：By step
(7) the gamma aminobutyric acid crystal collected dries 12 14h in 80 DEG C of baking oven, obtains the finished product of gamma aminobutyric acid.
Title:From the method for separating and purifying gamma-aminobutyric acid in glutaminase decarboxylase enzymolysis liquid, Application Number (patent):
CN201010207446.3.The applying date:2010.06.22.The invention is related to one kind and separates from Glutamic acid decarboxylase enzymolysis liquid
The method of purifying GABA (GABA), the method are comprised the following steps：Glutaminase decarboxylase enzymolysis liquid rich in GABA
Filtration clarification is carried out into film separation system, when being concentrated to 2～10 times, plus water-dialyzing dialysis, final amount of filtrate is 1～3 times of enzyme
Solution liquid measure；Filtrate is pumped into into ion exchange system desalination, desalinization liquor enters decolorizing resin or activated carbon column decoloring, clear after decolouring
Liquid obtains GABA products by evaporimeter concentration, drying or crystallization and recrystallization.Compared with prior art, the present invention
Separation purifying technique it is simple, rationally, operation is short, easy to operate, and isolated GABA purity is high, of light color, good dispersion,
Total recovery is high；Have the advantages that operating cost is low, filtering accuracy is high, cycles of concentration is high, enzyme concentrate concentration is high, be conducive to enzyme dense
The recycling of contracting thing or solidification recycling, it is to avoid secondary pollution.Principal claim:From glutaminase decarboxylase enzymolysis liquid
The method of separating and purifying gamma-aminobutyric acid, it is characterised in that the method is comprised the following steps：Will be enriched in the paddy of GABA
Aminoacyl ammonia decarboxylase enzymolysis liquid carries out filtration clarification into film separation system, when being concentrated to 2～10 times, plus water-dialyzing dialysis, most
Whole amount of filtrate is 1～3 times of fermentation liquid measure；Filtrate is pumped into into ion exchange system desalination, desalinization liquor enters decolorizing resin or activity
Charcoal post is decolourized, and the clear liquid after decolouring is concentrated by evaporimeter, and solid concentration reaches 30%～45%, concentrate drying
Device is dried or crystallizes and recrystallize, and obtains GABA product.
Above in the report that relevant lactic acid bacteria produces GABA, purifying, refined gamma-amino fourth from zymotic fluid
The step of acid includes：The technological process such as zymotic fluid decolouring, cation seperation column absorption, ammoniacal liquor wash-out, concentrated in vacuo, ethanol precipitation, tool
There are complex process, high labor intensive, wherein ammoniacal liquor, the use of ethanol easily causes production environment badly, and have
Very big potential safety hazard.Therefore, it is necessary to be directed to the purification of GABA in zymotic fluid, exploitation one kind can shorten production
Link, reduce labour cost, the novel production process for improving production environment, eliminating safe hidden trouble.
The content of the invention
In order to solve at present fermentation high labor intensive, production environment difference present in GABA purifying process with
And potential safety hazard it is high the problems such as, the present invention provide it is a kind of from zymotic fluid separate, purifying GABA method.
To solve above-mentioned technical problem, one aspect of the present invention is：
A kind of method that GABA is purified from zymotic fluid, the method are comprised the following steps：
(1) preparation and pretreatment of zymotic fluid：Lactobacillus brevis SC221 fermentations prepare the zymotic fluid containing GABA, so
Filtration sterilization is carried out to zymotic fluid with ceramic membrane afterwards, obtain except fermented liquid；
(2) cation seperation column absorption：Watery hydrochloric acid is pre-processed to acid strong cationic resin, obtains hydrogen form cation tree
Fat, then with hydrogen form cation resin adsorption except the GABA in fermented liquid；
(3) dilute alkaline soln wash-out：The GABA adsorbed by hydrogen form cation resin is washed with dilute alkaline soln
It is de-, collect GABA eluent；
(4) anion column decolourizes：Watery hydrochloric acid is pre-processed to basic anionic resin, obtains chlorine type resin anion (R.A.),
Then GABA eluent is decolourized with chlorine type resin anion (R.A.), obtains GABA efflux；
(5) electrodialysis desalination：Electrodialysis desalination is carried out to GABA efflux, desalination GABA is collected；
(6) concentrate and be dried：NF membrane reverse osmosis concentration is carried out to desalination GABA, trapped fluid is collected, is dried,
Solid-state GABA is obtained.
Lactobacillus brevis SC221, has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserves
Numbering is CGMCC No 11437.
Watery hydrochloric acid generally refers to the hydrochloric acid that mass percentage concentration is less than 10%.
It is as the further improvement of technical scheme, in the preparation of above-described zymotic fluid and pre-treatment step, described
The molecular weight of ceramic membrane is molecular weight 200-400kw, and the described electrical conductivity except fermented liquid is 30-50ms/cm.
Used as the further improvement of technical scheme, above-described hydrogen form cation resin adsorption is except in fermented liquid
The loading speed of GABA is 1-2BV/h.
Used as the further improvement of technical scheme, the concentration of the dilute alkaline soln in the above step (3) is 0.1-1mol/
L, described elution flow rate are 1-2BV/h.
As the further improvement of technical scheme, with chlorine type resin anion (R.A.) to gamma-amino fourth in the above step (4)
The loading speed decolourized by sour eluent is 1-2BV/h.
Used as the further improvement of technical scheme, in the above step (6), the film pressure of NF membrane reverse osmosis concentration is
0.5-1MPa。
Used as the further improvement of technical scheme, above-described electrodialysis desalination is to GABA efflux conductance
Rate stops desalination when being down to below 300 μ s/cm.
Used as the further improvement of technical scheme, above-described alkali is NaOH, the one kind in potassium hydroxide.
Used as the further improvement of technical scheme, above-described resin cation is 001*7.
Used as the further improvement of technical scheme, above-described resin anion (R.A.) is D201.
The invention has the advantages that：
1. degerming technique of the present invention using ceramic membrane, compared with prior art, with efficiently, easily advantage, be adapted to
In industrial applications.
2. the present invention before zymotic fluid decolouring first carried out resin cation and the selectivity of GABA is adsorbed.The step for
Be different from after the first decolouring in conventional technique and adsorb, this change can reduce protein in subsequent eluate, residual sugar and other
The content of the non-purpose thing such as amino acid, also so that the follow-up decolouring of eluent becomes more efficient.
3. the GABA adsorbed using other aqueous slkali wash-out resin cations by the present invention, replaces with volatile ammonia
Water, the step for can improve production environment compared with conventional technique well.
4. the present invention concentrates and is spray-dried production GABA finished products using NF membrane, replaces to ethanol precipitation in conventional technique
The steps such as GABA, deposit sample crystallization, this change can eliminate safe hidden trouble, and reduce production cost, and with conventional technique
Compare, can equally obtain the very high GABA product of purity.
Description of the drawings
Fig. 1：The process flow diagram of GABA is purified from zymotic fluid.
Specific embodiment
Below in conjunction with the accompanying drawings presently preferred embodiments of the present invention is described in detail, so that advantages and features of the invention energy
It is easier to be readily appreciated by one skilled in the art, apparent clearly defines so as to make to protection scope of the present invention.
It is prepared by the zymotic fluid of GABA：
Lactobacillus brevis SC221 (is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserves and compile
Number for CGMCC No 11437) ferment prepare contain GABA zymotic fluid, fermentation medium is the MRS containing sodium glutamate
Culture medium：Beef extract albumen powder 10g/L；Peptone 10g/L；Dusty yeast 5g/L；Sodium acetate 5g/L；K2HPO42g/L；Citric acid
Two ammonium 2g/L；Magnesium sulfate 0.2g/L；Manganese sulfate 0.2g/L；Tween-80 1mL/L；Glucose 50g/L；Sodium glutamate 20-50g/
L.Fermentation temperature is 35 DEG C, and mixing speed is 100rpm, controls pH 5.0, is passed through without air.
Fermentation ends obtain the zymotic fluid containing GABA.
A kind of method that GABA is purified from zymotic fluid
Alpha-aminobutyric acid content is detected：According to light industry of China standard QB/T 4587-2013 methods describeds, sample Jing is adjacent
After phthalaldehyde is derivative, detected with high performance liquid chromatography, chromatographic column is C18Post, detector are UV-detector.
Colorimetric determination decolorizing effect：Feed liquid is scanned in each wavelength, the position of its maximum absorption band is found out, with this
Investigation foundation of the absorbance change at wavelength as decolorizing effect.Percent of decolourization computing formula is as follows：Percent of decolourization %=(A0-A)/
A0× 100%.A0, A respectively decolourize before and after light absorption value.
Embodiment 1
(1) preparation and pretreatment of zymotic fluid：Lactobacillus brevis SC221 fermentations prepare the zymotic fluid containing GABA, so
It is that 200kw ceramic membranes carry out filtration sterilization to zymotic fluid with molecular cut off afterwards, obtain that electrical conductivity is 39.7ms/cm degerming
Zymotic fluid.After testing, the yield of GABA is 95.1%.
(2) cation seperation column absorption：The watery hydrochloric acid of concentration 3% is pre-processed to acid strong cationic resin 001*7, is obtained
Hydrogen form cation resin, then with hydrogen form cation resin adsorption except the GABA in fermented liquid, loading speed is
1.0 column volumes/hour (BV/h).
(3) dilute alkaline soln wash-out：It is 0.5mol/L diluted alkalines (NaOH) solution to hydrogen form cation resin institute with concentration
The GABA of absorption is eluted, and elution flow rate is 1.0BV/h, collects GABA eluent.Detection gamma-amino
Butyric acid yield, eluent electrical conductivity and percent of decolourization:Test result indicate that, the GABA rate of recovery is 97.3%, eluent electricity
Conductance is down to 6.9ms/cm, and compared with zymotic fluid, percent of decolourization is 66%.
(4) anion column decolourizes：Basic anionic resin D201 is pre-processed with the watery hydrochloric acid of concentration 3%, obtained
Chlorine type resin anion (R.A.), is then decolourized to GABA eluent with chlorine type resin anion (R.A.), the loading speed of decolouring
For 1.0BV/h, GABA efflux is obtained.Detection GABA yield and percent of decolourization:Test result indicate that, it is cloudy from
In sub- post decolorization, the GABA rate of recovery is 92.1%, compared with zymotic fluid, percent of decolourization>95%.
(5) electrodialysis desalination：Electrodialysis desalination, GABA efflux conductance are carried out to GABA efflux
Rate stops desalination when being down to below 300 μ S/cm, collect desalination GABA.The detection GABA rate of recovery:As a result table
Bright, during electrodialysis desalination, GABA yield is 95.6%.
(6) concentrate and be dried：NF membrane reverse osmosis concentration, the mould of reverse osmosis concentration are carried out to desalination GABA
Power remains 0.5MPa, collects trapped fluid (the detection GABA rate of recovery:As a result show, γ during electrodialysis desalination-
Aminobutyric acid yield is 89.5%.), it is spray-dried, you can obtain solid-state GABA finished product.To contain GABA
On the basis of zymotic fluid, GABA yield>70%, percent of decolourization>95%, gained GABA purity>97%.
Embodiment 2
(1) preparation and pretreatment of zymotic fluid：Lactobacillus brevis SC221 fermentations prepare the zymotic fluid containing GABA, so
It is that 300kw ceramic membranes carry out filtration sterilization to zymotic fluid with molecular cut off afterwards, obtain that electrical conductivity is 48.6ms/cm degerming
Zymotic fluid, the yield of GABA is 93.7%.
(2) cation seperation column absorption：The watery hydrochloric acid of concentration 5% is pre-processed to acid strong cationic resin 001*7, is obtained
Hydrogen form cation resin, then with hydrogen form cation resin adsorption except the GABA in fermented liquid, loading speed is
1.5 column volumes/hour (BV/h).
(3) dilute alkaline soln wash-out：It is 0.8mol/L diluted alkalines (potassium hydroxide) solution to hydrogen form cation resin institute with concentration
The GABA of absorption is eluted, and elution flow rate is 1.5BV/h, collects GABA eluent.Detection gamma-amino
Butyric acid yield, eluent electrical conductivity and percent of decolourization：Test result indicate that, the GABA rate of recovery is 95.4%, eluent electricity
Conductance is down to 8.8ms/cm, and compared with zymotic fluid, percent of decolourization is 71%.
(4) anion column decolourizes：Basic anionic resin D201 is pre-processed with the watery hydrochloric acid of concentration 3%, obtained
Chlorine type resin anion (R.A.), is then decolourized to GABA eluent with chlorine type resin anion (R.A.), the loading speed of decolouring
For 1.5BV/h, GABA efflux is obtained.Detection GABA yield and percent of decolourization:Test result indicate that, it is cloudy from
In sub- post decolorization, the GABA rate of recovery is 93.8%, compared with zymotic fluid, percent of decolourization>95%.
(5) electrodialysis desalination：Electrodialysis desalination, GABA efflux conductance are carried out to GABA efflux
Rate stops desalination when being down to below 250 μ S/cm, collect desalination GABA.The detection GABA rate of recovery：As a result table
Bright, during electrodialysis desalination, GABA yield is 94.9%.
(6) concentrate and be dried：NF membrane reverse osmosis concentration, the mould of reverse osmosis concentration are carried out to desalination GABA
Power remains 0.8MPa, collects trapped fluid (the detection GABA rate of recovery:As a result show, γ during electrodialysis desalination-
Aminobutyric acid yield is 91.7%.), it is spray-dried, you can obtain solid-state GABA finished product.To contain GABA
On the basis of zymotic fluid, GABA yield>70%, percent of decolourization>95%, gained GABA purity>95%.
Embodiment 3
(1) preparation and pretreatment of zymotic fluid：Lactobacillus brevis SC221 fermentations prepare the zymotic fluid containing GABA, so
Afterwards with being that 400kw ceramic membranes carry out filtration sterilization to zymotic fluid with molecular weight, the degerming fermentation that electrical conductivity is 31.6ms/cm is obtained
Liquid.The yield of GABA is 96.3%.
(2) cation seperation column absorption：The watery hydrochloric acid of concentration 8% is pre-processed to acid strong cationic resin 001*7, is obtained
Hydrogen form cation resin, then with hydrogen form cation resin adsorption except the GABA in fermented liquid, loading speed is
2.0 column volumes/hour (BV/h).
(3) dilute alkaline soln wash-out：It is 1.0mol/L diluted alkalines (NaOH) solution to hydrogen form cation resin institute with concentration
The GABA of absorption is eluted, and elution flow rate is 1.5BV/h, collects GABA eluent.Detection gamma-amino
Butyric acid yield, eluent electrical conductivity and percent of decolourization：Test result indicate that, the GABA rate of recovery is 96.9%, eluent electricity
Conductance is down to 6.7ms/cm, and compared with zymotic fluid, percent of decolourization is 68%.
(4) anion column decolourizes：Anion column decolourizes：Basic anionic resin D201 is entered with the watery hydrochloric acid of concentration 3%
Row pretreatment, obtains chlorine type resin anion (R.A.), then GABA eluent is decolourized with chlorine type resin anion (R.A.),
The loading speed of decolouring is 2.0BV/h, obtains GABA efflux.Detection GABA yield and percent of decolourization：It is real
Test result to show, in anion column decolorization, the GABA rate of recovery is 92.6%, compared with zymotic fluid, percent of decolourization>
95%.
(5) electrodialysis desalination：Electrodialysis desalination, GABA efflux conductance are carried out to GABA efflux
Rate stops desalination when being down to below 200 μ S/cm, collect desalination GABA.The detection GABA rate of recovery：As a result table
Bright, during electrodialysis desalination, GABA yield is 96.6%.
(6) concentrate and be dried：NF membrane reverse osmosis concentration, the mould of reverse osmosis concentration are carried out to desalination GABA
Power remains 1.0MPa, collects trapped fluid (the detection GABA rate of recovery:As a result show, γ during electrodialysis desalination-
Aminobutyric acid yield is 87.8%.), it is spray-dried, you can obtain solid-state GABA finished product.To contain GABA
On the basis of zymotic fluid, GABA yield>70%, percent of decolourization>95%, gained GABA purity>98%.
In above example, electrodialysis desalination needs to adjust GABA efflux pH to 7.0-7.5.
Embodiments of the invention are the foregoing is only, the scope of the claims of the present invention is not thereby limited, it is every using this
Equivalent structure or equivalent flow conversion that bright specification and accompanying drawing content are made, or directly or indirectly it is used in other related skills
Art field, is included within the scope of the present invention.
Claims (10)
1. it is a kind of from zymotic fluid purify GABA method, it is characterised in that：The method is comprised the following steps：
(1) preparation and pretreatment of zymotic fluid：Lactobacillus brevis SC221 fermentations prepare the zymotic fluid containing GABA, Ran Houyong
Ceramic membrane carries out filtration sterilization to zymotic fluid, obtain except fermented liquid；
(2) cation seperation column absorption：Watery hydrochloric acid is pre-processed to acid strong cationic resin, obtains hydrogen form cation resin, so
Afterwards with hydrogen form cation resin adsorption except the GABA in fermented liquid；
(3) dilute alkaline soln wash-out：The GABA adsorbed by hydrogen form cation resin is eluted with dilute alkaline soln, received
Collection GABA eluent；
(4) anion column decolourizes：Watery hydrochloric acid is pre-processed to basic anionic resin, obtains chlorine type resin anion (R.A.), then
GABA eluent is decolourized with chlorine type resin anion (R.A.), obtain GABA efflux；
(5) electrodialysis desalination：Electrodialysis desalination is carried out to GABA efflux, desalination GABA is collected；
(6) concentrate and be dried：NF membrane reverse osmosis concentration is carried out to desalination GABA, trapped fluid is collected, is dried, you can
Obtain solid-state GABA.
2. it is according to claim 1 it is a kind of from zymotic fluid purify GABA method, it is characterised in that：It is described
Zymotic fluid preparation and pre-treatment step in, the molecular weight of described ceramic membrane is 200-400kw, described except fermented liquid
Electrical conductivity be 30-50ms/cm.
3. it is according to claim 1 and 2 it is a kind of from zymotic fluid purify GABA method, it is characterised in that：Institute
The hydrogen form cation resin adsorption stated is 1-2BV/h except the loading speed of the GABA in fermented liquid.
4. it is according to claim 3 it is a kind of from zymotic fluid purify GABA method, it is characterised in that：It is described
The concentration of the dilute alkaline soln in step (3) is 0.5-1mol/L, and described elution flow rate is 1-2BV/h.
5. it is according to claim 1 and 2 it is a kind of from zymotic fluid purify GABA method, it is characterised in that：Institute
The loading speed decolourized to GABA eluent with chlorine type resin anion (R.A.) in stating step (4) is 1-2BV/h.
6. it is according to claim 1 and 2 it is a kind of from zymotic fluid purify GABA method, it is characterised in that：Institute
The film pressure for stating NF membrane reverse osmosis concentration in step (6) is 0.5-1MPa.
7. it is according to claim 1 and 2 it is a kind of from zymotic fluid purify GABA method, it is characterised in that：Institute
The electrodialysis desalination stated stops desalination when being down to below 300 μ s/cm to GABA efflux electrical conductivity.
8. a kind of method that according to claim 1 or 4 purifies GABA from zymotic fluid, it is characterised in that：Institute
The alkali stated is NaOH, the one kind in potassium hydroxide.
9. it is according to claim 1 it is a kind of from zymotic fluid purify GABA method, it is characterised in that：It is described
Resin cation model 001*7.
10. it is according to claim 1 it is a kind of from zymotic fluid purify GABA method, it is characterised in that：It is described
Resin anion (R.A.) be D201.
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| CN109761832A (en) * | 2019-03-05 | 2019-05-17 | 内蒙古精晶生物科技有限公司 | A kind of purification process of 4-Aminobutanoicacid |
| CN113214160A (en) * | 2021-05-27 | 2021-08-06 | 无锡晶海氨基酸股份有限公司 | Method for efficiently purifying histidine bulk drug without ammonia nitrogen discharge |
| CN114014771A (en) * | 2021-06-30 | 2022-02-08 | 安徽华恒生物科技股份有限公司 | Ultrahigh-purity amino acid, and preparation method and application thereof |
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| CN114058653A (en) * | 2021-12-29 | 2022-02-18 | 南通励成生物工程有限公司 | Method for preparing gamma-aminobutyric acid through biosynthesis method |
| CN114058653B (en) * | 2021-12-29 | 2024-02-09 | 南通励成生物工程有限公司 | Method for preparing gamma-aminobutyric acid by biological synthesis method |
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