CN210065780U - Circulating tumor cell detection device - Google Patents
Circulating tumor cell detection device Download PDFInfo
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- CN210065780U CN210065780U CN201920758570.5U CN201920758570U CN210065780U CN 210065780 U CN210065780 U CN 210065780U CN 201920758570 U CN201920758570 U CN 201920758570U CN 210065780 U CN210065780 U CN 210065780U
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Abstract
The utility model discloses a circulating tumor cell detection device, which comprises a blood sampling reflux device, a centrifuge, a dyeing device and a detection mechanism which are connected in sequence; the blood collection reflux device comprises a blood collection pipeline, a three-way control valve, a blood collector, a blood collection bag, a cell collection filter and a blood return pipeline, wherein the blood collection pipeline is externally connected with an anticoagulation factor pipeline through a valve; the cell collection filter comprises a filter body, a blood sample inlet is connected with a blood sample at the top end of the filter body, a sealing ring is arranged between the blood sample inlet and the top end of the filter body, and a fence type filter device is arranged at the center of the sealing ring; blood sample access mouth passes through the clip with the sealing washer and presss from both sides as an organic wholely, has seted up pressure measurement mouth and vacuum pump interface on the filter body lateral wall. The utility model discloses can gather the tumor cell of capacity and carry out the analysis, it is few to the patient injury, separate with collect effectual, detect the reliability height after dyeing, false positive is few, has improved the precision of testing result.
Description
Technical Field
The utility model belongs to the technical field of medical instrument, concretely relates to circulating tumor cell detection device.
Background
Circulating Tumor Cells (CTCs) are cancer cells that have detached from the tumor and entered the blood circulation, and the progression of the disease and the therapeutic effect of a cancer patient can be assessed by detecting the number of circulating tumor cells. However, since the circulating tumor cells have very low abundance in peripheral blood, about 0.3-100/ml, which is 4-7 orders of magnitude different from that of leukocytes, in practical application, the amount of CTCs enriched in 5-10ml of blood drawn each time is basically incomplete or difficult to complete, and repeated blood drawing for detection also results in large blood loss, which adversely affects the body of a patient. In addition, most of the enrichment sorting methods of the circulating tumor cells have other defects, such as membrane sorting and acoustic sorting are easy to damage the cell activity; the extrusion flow sorting can obtain a better sorting effect only when the sizes of two types of particles are greatly different; magnetic, acoustic, optical, electrical sorting introduces energy-consuming external fields, which makes the equipment expensive and complex to operate.
SUMMERY OF THE UTILITY MODEL
The utility model discloses the technical problem who solves: the circulating tumor cell detection device can collect sufficient tumor cells for analysis, has less harm to patients, good separation and collection effects, high detection reliability after dyeing and less false positive.
In order to solve the technical problem, the technical scheme of the utility model is that:
a circulating tumor cell detection device comprises a blood sampling reflux device, a centrifuge, a dyeing device and a detection mechanism which are connected in sequence;
the blood collection backflow device comprises a blood collection pipeline, a three-way control valve, a blood collector, a blood collection bag, a cell collection filter and a blood feedback pipeline, wherein the three-way control valve is respectively communicated with the blood collection pipeline, the blood collection bag and the blood feedback pipeline which are arranged in the blood collector; the blood collection pipeline is externally connected with an anticoagulation factor pipeline through a valve;
the cell collection filter comprises a filter body, a blood sample inlet is connected to a blood sample position at the top end of the filter body, a sealing ring is arranged between the blood sample inlet and the top end of the filter body, and a fence type filter device is arranged in the center of the sealing ring; blood sample access mouth passes through the clip with the sealing washer and presss from both sides as an organic wholely, has seted up pressure measurement mouth and vacuum pump interface on the filter body lateral wall, and pressure measurement mouth links to each other with external pressure measurement appearance, and vacuum pump interface is external through the vacuum control valve has the vacuum pump.
Preferably, the position of the blood collection bag relative to the collection pipeline and the blood return pipeline is changed by manual operation or a lifting device.
Preferably, the fence type filter device is oblate and 8mm in diameter, and contains about 5 thousand independent small fences with the length and the width of each 100 micrometers; the barrier gap of the small barrier is 4-7 microns (preferably 5 microns, and the best separation effect can be achieved), and the barrier type filter device is made of polystyrene materials.
Preferably, a liquid stopping clamp is further arranged between the blood collecting bag and the cell collecting filter, and the liquid stopping clamp is a clamp body formed by two mutually hinged clamping arms; the blood return pipeline is also provided with a flow speed controller.
Preferably, the centrifuge is a table centrifuge, Thermo, Legend Micro17, U.S. and the rotating speed range is 0-20000 r/min.
Preferably, the dyeing device is a fluorescent dyeing kit which is filled with a fluorescent dyeing reagent, wherein the fluorescent dyeing reagent is Hoechst3325 fluorescent dye and is purchased from Shanghai Bin Yuntian Biotech Co.
Preferably, the detection mechanism comprises a laser electron microscope and a high-throughput cell image analyzer, and the laser electron microscope is electrically connected with the high-throughput cell image analysis system. Wherein the laser electron microscope is a laser scanning confocal microscope AI, Nikon corporation of Japan; the high-throughput cell image analysis system is a Roche real-time high-throughput cell analysis system, and has the model number: xcelligene.
Since the technical scheme is used, the beneficial effects of the utility model are that:
1. the utility model discloses a blood feedback's setting can collect circulating tumor cell through a large amount of blood samplings, has guaranteed the required quantity of circulating tumor cell of experiment, and simultaneously, the feedback of blood does not bring adverse effect to patient's health again.
2. The utility model discloses blood sampling reflux unit's configuration makes whole collection process be convenient for control, effectively prolongs the mixing time of blood and anticoagulant, has avoided in blood sampling and the disengaging process because blood condenses and form the jam.
3. The utility model discloses a barrier formula filter equipment's separation principle not be based on tumor cell size, but come the separation CTCs based on tumor cell nucleus size, according to lie in that the tumor cell nucleus is big, the deformability is poor, and the form is unusual, compares with the whole size of cell, and the diameter variability of tumor cell nucleus is littleer, consequently, compares with traditional micropore array, the utility model discloses the porosity of barrier formula structure is bigger for filtration efficiency is higher, weak point consuming time, and the pressure that the cell bore when filtering is littleer.
4. The utility model discloses a centrifuge will filter the tumor cell further separation and the enrichment of collecting, can obtain purer tumor cell as far as for the dyeing and the detection of procedure at the back.
In a word, the utility model discloses can gather the tumor cell of capacity and carry out the analysis, it is few to the patient injury, and separation and collection effect are good, and it is high to detect the reliability after dyeing, and false positive is few, has improved the precision of testing result.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic structural view of a cell collection filter according to an embodiment of the present invention;
wherein, 1-the blood collecting vessel; 2-a three-way control valve; 3-a blood sampling instrument; 4-blood collection bag; 5-cell collection filter; 51-a filter body; 52-blood sample access; 53-sealing ring; 54-barrier filter devices; 55-a clamp; 56-pressure sensing port; 57-vacuum pump interface; 6-blood return line; 7-a valve; 8-an anti-coagulation factor conduit; 9-liquid stopping clip; 10-a flow rate controller; 11-a centrifuge; 12-a dyeing device; 13-detection mechanism.
Detailed Description
The invention is further explained below with reference to the drawings and examples.
As shown in fig. 1 and fig. 2, a circulating tumor cell detecting device comprises a blood collecting reflux device, a centrifuge 11, a staining device 12 and a detecting mechanism 13 which are connected in sequence;
the blood collection backflow device comprises a blood collection pipeline 1, a three-way control valve 2, a blood collector 3, a blood collection bag 4, a cell collection filter 5 and a blood feedback pipeline 6, wherein the three-way control valve 2 is respectively communicated with the blood collection pipeline 1, the blood collection bag 4 and the blood feedback pipeline 6 which are arranged in the blood collector 3, a blood inlet of the blood collection bag 4 is communicated with the three-way control valve 2 through a pipeline, a blood outlet of the blood collection bag 4 is communicated with the cell collection filter 5 through a pipeline, and the cell collection filter 5 is communicated with the three-way control valve 2 through the blood feedback pipeline 6; the blood collection pipeline 1 is externally connected with an anticoagulation factor pipeline 8 through a valve 7;
the cell collection filter 5 comprises a filter body 51, a blood sample inlet 52 is connected to a blood sample position at the top end of the filter body 51, a sealing ring 53 is arranged between the blood sample inlet 52 and the top end of the filter body 51, and a fence type filter device 54 is arranged at the center of the sealing ring 53; blood sample access port 52 and sealing washer 53 press from both sides as an organic wholely through clip 55, have seted up pressure measurement mouth 56 and vacuum pump interface 57 on the filter body 51 lateral wall, and pressure measurement mouth 56 links to each other with external pressure measurement appearance, and vacuum pump interface 57 is external through the vacuum control valve has the vacuum pump.
A liquid stopping clamp 9 is also arranged between the blood collecting bag 4 and the cell collecting filter 5, and the liquid stopping clamp 9 is a clamp body formed by two mutually hinged clamping arms; the blood return line 6 is also provided with a flow rate controller 10.
Wherein, the fence type filter device 54 is oblate, has a diameter of 8mm, and contains about 5 thousand independent small fences with length and width of 100 μm; the barrier gap of the small barrier is 5 microns, and the barrier type filter device 54 is made of polystyrene material.
When the blood collecting device is actually used, the existing liquid stopping clamp 9 closes a pipeline between the blood collecting bag 4 and the cell collecting filter 5, then the three-way control valve 2 is opened, blood is collected for a patient through the blood collecting pipeline 1, the valve 7 is opened while blood is collected, and the anticoagulation factor is added through the anticoagulation factor pipeline 8, so that the blood is prevented from being coagulated in vitro for a long time; after a certain amount of blood is collected, opening the liquid stopping clamp 9, conveying the blood into the cell collecting filter 5, carrying out primary separation on the blood through the barrier type filter device 54 by adopting negative pressure, returning the separated blood into the body of a patient through the blood return pipeline 6, controlling the flow rate through the flow rate controller 10 in the return process, closing the valve 7, and closing a channel leading the three-way control valve 2 to the blood sampling instrument 3;
after separation, the tumor cells obtained on the fence type filter device 54 are eluted by eluent, then the centrifuge 11 is used for secondary separation and purification by centrifugal effect generated by different rotating speeds, the obtained tumor cells are transferred into a staining kit and stained by Hoechst3325 fluorescent dye, and after staining, detection is carried out by a laser scanning confocal microscope and a high-throughput cell analysis system.
The circulating tumor cells are marked by the fluorescent staining agent, the marked circulating tumor cells acquire distribution images of the circulating tumor cells by using a laser scanning confocal microscope, the images are transmitted to a high-throughput cell analysis system, the number of the circulating tumor cells is obtained by using the high-throughput cell image analysis system, and the reliability is high.
It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the teachings of the present invention, and these equivalents also fall within the scope of the appended claims.
Claims (6)
1. A circulating tumor cell detection device, characterized in that: comprises a blood sampling reflux device, a centrifuge, a dyeing device and a detection mechanism which are connected in sequence;
the blood collection backflow device comprises a blood collection pipeline, a three-way control valve, a blood sampling instrument, a blood collection bag, a cell collection filter and a blood feedback pipeline, wherein the three-way control valve is respectively communicated with the blood collection pipeline, the blood collection bag arranged in the blood sampling instrument and the blood feedback pipeline; the cell collection filter is communicated with the three-way control valve through a blood return pipeline; an anticoagulant factor pipeline is externally connected to the blood collection pipeline through a valve;
the cell collection filter comprises a filter body, a blood sample inlet is connected to a blood sample position at the top end of the filter body, a sealing ring is arranged between the blood sample inlet and the top end of the filter body, and a fence type filtering device is arranged in the center of the sealing ring; blood sample access mouth passes through the clip with the sealing washer and presss from both sides as an organic wholely, pressure measurement mouth and vacuum pump interface have been seted up on the filter body lateral wall, pressure measurement mouth links to each other with external pressure measurement appearance, vacuum pump interface is external through the vacuum control valve has the vacuum pump.
2. The circulating tumor cell detecting apparatus according to claim 1, wherein: the position of the blood collection bag relative to the collection pipeline and the blood return pipeline is changed by manual operation or a lifting device.
3. The circulating tumor cell detecting apparatus according to claim 1, wherein: the fence type filter device is oblate, has a diameter of 8mm, and contains about 5 thousand independent small fences with the length and the width of 100 mu m; the barrier gap of the small barrier is 4-7 microns.
4. The circulating tumor cell detecting apparatus according to claim 1, wherein: still be provided with between blood collection bag and the cell collection filter and end the liquid and press from both sides, still be provided with flow rate controller on the blood feedback pipeline.
5. The circulating tumor cell detecting apparatus according to claim 1, wherein: the dyeing device is a fluorescent dyeing kit.
6. The circulating tumor cell detecting apparatus according to claim 1, wherein: the detection mechanism comprises a laser electron microscope and a high-flux cell image analysis system, and the fluorescence electron microscope is electrically connected with the high-flux cell image analysis system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920758570.5U CN210065780U (en) | 2019-05-24 | 2019-05-24 | Circulating tumor cell detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920758570.5U CN210065780U (en) | 2019-05-24 | 2019-05-24 | Circulating tumor cell detection device |
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| CN210065780U true CN210065780U (en) | 2020-02-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111904435A (en) * | 2020-07-20 | 2020-11-10 | 连云港市第一人民医院 | Be used for detecting hematopathy mutant cell selective examination nursing device |
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2019
- 2019-05-24 CN CN201920758570.5U patent/CN210065780U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111904435A (en) * | 2020-07-20 | 2020-11-10 | 连云港市第一人民医院 | Be used for detecting hematopathy mutant cell selective examination nursing device |
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Granted publication date: 20200214 Termination date: 20210524 |
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| CF01 | Termination of patent right due to non-payment of annual fee |