CN208125478U - LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine - Google Patents
LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine Download PDFInfo
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- CN208125478U CN208125478U CN201820649609.5U CN201820649609U CN208125478U CN 208125478 U CN208125478 U CN 208125478U CN 201820649609 U CN201820649609 U CN 201820649609U CN 208125478 U CN208125478 U CN 208125478U
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Abstract
The utility model discloses a kind of LCT Thinprep pap test and the quick film-makings of cell DNA and dyeing all-in-one machine, including cabinet, motor is equipped in the middle part of the intracorporal bottom wall of case, the output end of the motor is coaxially connected with rotation axis, the rotation axis top is in the middle part of partition and end is coaxially connected with centrifugation frame, the roof of the centrifugation frame is equipped with cyclic annular lifting groove, bottom wall two sides in the lifting groove are equipped with cylinder, the output end of two cylinders is equipped with telescopic rod, two telescopic rod top ends are fixedly connected with cyclic rings type frame, ring-like lens channel is equipped in the roof of the ring-like frame, observation piece is equipped in lens channel described in two groups, the cabinet top is equipped with case lid, the case lid bottom end is respectively equipped with No.1 cricoid cartilage and No. two cricoid cartilages.The utility model is easy to use, and dyeing effect is good, can once carry out multiple groups sectioning cells and dyeing work, promote working efficiency.
Description
Technical field
The utility model relates to technical field of medical equipment more particularly to a kind of LCT Thinprep pap test and cell DNA are fast
Fast film-making and dyeing all-in-one machine.
Background technique
Liquid based cytology is using liquid-based thin-layer cell detection system (LCT) detection cervical cell and carries out cytology specification sieve
Choosing and diagnosis are more advanced a kind of cervical cancer cell Examined effects in the world at present, with traditional papanicolaou test phase
Than, hence it is evident that the satisfaction and abnormal cervical cell recall rate of sample are improved, uterine neck anti-cancer cytolgical examination is not only can be found that
Cervical carcinoma and precancerous lesion can also detect microorganism infection such as mould, trichomonad, virus, Chlamydia etc..Liquid-based technology is application
In an advanced technology of woman uterus cancer screening.Therefore, LCT liquid basal cell system is collected to sample from clinician
Piece, each step among these is all particularly critical for making high-quality cell sheet, and LCT Thinprep pap test and cell DNA are quickly made
Piece and dyeing all-in-one machine can supplement the deficiency of conventional cytology or pathological diagnosis method, pathologist can be helped to tumprigenicity greatly
The judgement of matter makes it according to the above analysis, makes the early diagnosis report of early stage (uterine neck) cancer and precancerous lesion screening respectively.
Existing LCT Thinprep pap test and the quick film-making of cell DNA and dyeing integrative machine have the following disadvantages it
Place：Existing apparatus working efficiency is low, and the film-making time is long and quality not can guarantee, and dyeing effect is poor, the feelings for the sample that is easily damaged
Condition, causes cell DNA to quantify the lag of ploidy analysis report, and the state of an illness for influencing patient is made a definite diagnosis and treated.
Utility model content
The purpose of this utility model is to provide a kind of LCT Thinprep pap test and the quick film-makings of cell DNA and dyeing one
Body machine, has easy to use, can once carry out multiple groups sectioning cells and dyeing work, the advantages of work efficiency is high solves existing
There is device dyeing effect poor, the problem of film-making time length.
According to a kind of LCT Thinprep pap test of the utility model embodiment and the quick film-making of cell DNA and dyeing one
Machine, including cabinet, the intracorporal bottom wall middle part of case are equipped with motor, and the output end of the motor is coaxially connected with rotation axis, institute
Rotation axis top is stated in the middle part of partition and end is coaxially connected with centrifugation frame, the roof of the centrifugation frame is equipped with cyclic annular lifting
Slot, the bottom wall two sides in the lifting groove are equipped with cylinder, and the output end of two cylinders is equipped with telescopic rod, described in two
Telescopic rod top end is fixedly connected with cyclic rings type frame, and ring-like lens channel, eyeglass described in two groups are equipped in the roof of the ring-like frame
Observation piece is equipped in slot, the cabinet top is equipped with case lid, and the case lid bottom end is respectively equipped with No.1 cricoid cartilage and No. two
The side liquid feeding end of cricoid cartilage, the No.1 hose and No. two hoses run through case lid bottom wall and with the No.1 that is located in case lid
Micro pump communicates, and the input terminal of the No.1 micro pump is communicated with No.1 fluid-through tube, is equipped with No.1 control in the No.1 fluid-through tube
The right end of liquid valve, the No.1 fluid-through tube is connected with spray tank, the other side liquid pumping side of the No.1 hose and No. two hoses
Run through case lid bottom wall and communicated with water main, No. two liquid-controlling valves, the end of the water main and two are equipped in the water main
The input terminal of number micro pump communicates, and the output end of No. two micro pumps is equipped with No. two fluid-through tubes, the end of No. two fluid-through tubes
End is communicated through case lid bottom wall and with leakage fluid dram.
Further, the No.1 hose is fixedly connected by fixing buckle with case lid bottom wall with No. two hoses.
Further, the bottom end of the No.1 hose and No. two hoses is placed with multiple droppers, the dropper respectively with
No.1 hose, No. two hoses communicate.
Further, the observation piece roof is equipped with titration chamber.
Further, it is equipped with groove in the middle part of the roof of the centrifugation frame, is equipped in the groove and holds liquid bucket.
Further, the spray tank is fixed on case lid inner roof wall.
The utility model has an advantageous effect in that compared with prior art：
1, intracavitary to titrating by being provided with No.1 micro pump, No. two micro pumps, No.1 hose, No. two hoses, dropper
Medical fluid is injected and is drawn, and keeps equipment use more convenient, while promoting dyeing quality and film-making efficiency, when reducing film-making
Between, made sample tablet quality is stable, cell is complete, is evenly distributed, and film-making and dyeing efficiency are high；
2, sample disposal, system can be realized by being provided with motor, ring-like frame, holding liquid bucket, No.1 micro pump, No. two micro pumps
Piece, dyeing are once completed in an instrument, it can be achieved that quickly making with machine simultaneously to sample LCT Thinprep pap test and cell DNA
Piece and dyeing, accelerate film-making and dyeing time, that reduces doctor makes a definite diagnosis the time, enables the patient to obtain the effective treatment as early as possible.
Detailed description of the invention
Attached drawing is used to provide a further understanding of the present invention, and constitutes part of specification, practical with this
Novel embodiment is used to explain the utility model together, does not constitute limitations of the present invention.In the accompanying drawings：
Fig. 1 be the utility model proposes a kind of LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine
Structural schematic diagram；
Fig. 2 be the utility model proposes a kind of LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine
Cabinet top view；
Fig. 3 be the utility model proposes a kind of LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine
Case lid bottom view.
In figure：1- cabinet, 2- motor, 3- partition, 4- centrifugation frame, that 5- holds liquid bucket, 6- cylinder, 7- telescopic rod, 8- is ring-like
Frame, 9- case lid, 10- No.1 hose, No. bis- hoses of 11-, 12- fixing buckle, 13- dropper, 14- No.1 micro pump, 15- No.1 draining
Valve, 16- spray tank, No. bis- micro pumps of 17-, 18- observe piece, 19- leakage fluid dram.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Referring to Fig.1-3, a kind of LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine, including cabinet 1,
Motor 2 is equipped in the middle part of bottom wall in cabinet 1, the output end of motor 2 is coaxially connected with rotation axis, and partition 3 is run through on rotation axis top
Middle part and end are coaxially connected with centrifugation frame 4, and the roof of centrifugation frame 4 is equipped with cyclic annular lifting groove, and the bottom wall two sides in lifting groove are equal
Equipped with cylinder 6, the output end of two cylinders 6 is equipped with telescopic rod 7, two 7 tops of telescopic rod and the fixed company of cyclic rings type frame 8
It connects, ring-like lens channel is equipped in the roof of ring-like frame 8, observation piece 18 is equipped in two groups of lens channels, 1 top of cabinet is equipped with case lid
9,9 bottom end of case lid is respectively equipped with No.1 cricoid cartilage 10 and No. two cricoid cartilages 11, and the one of No.1 hose 10 and No. two hoses 11
Side liquid feeding end runs through 9 bottom wall of case lid and communicates with the No.1 micro pump 14 being located in case lid, the type that No.1 micro pump 14 uses
It number is BasePump, the input terminal of No.1 micro pump 14 is communicated with No.1 fluid-through tube, and No.1 liquid-controlling valve is equipped in No.1 fluid-through tube
15, the right end of No.1 fluid-through tube is connected with spray tank, and No.1 hose 10 and the other side liquid pumping side of No. two hoses 11 are run through
9 bottom wall of case lid is simultaneously communicated with water main, is equipped with No. two liquid-controlling valves in water main, and the end of water main is defeated with No. two micro pumps 17
Enter end to communicate, the output ends of No. two micro pumps 17 is equipped with No. two fluid-through tubes, the ends of No. two fluid-through tubes through 9 bottom wall of case lid and with
Leakage fluid dram 19 communicates；By being provided with 14, No. two micro pumps 17 of No.1 micro pump, No.1 hose 10, No. two hoses 11, dropper
13 pairs of intracavitary medical fluids of titration are injected and are drawn, and keep equipment use more convenient, while promoting dyeing quality and film-making effect
Rate reduces the film-making time, and made sample tablet quality is stable, cell is complete, is evenly distributed, and film-making and dyeing efficiency are high.
No.1 hose 10 is fixedly connected by fixing buckle 12 with 9 bottom wall of case lid with No. two hoses 11, No.1 hose 10 and two
The bottom end of number hose 11 is placed with multiple droppers 13, and dropper 13 is communicated with 10, No. two hoses 11 of No.1 hose respectively, observation
18 roof of piece is equipped with titration chamber, is centrifuged in the middle part of the roof of frame 4 and is equipped with groove, is equipped in groove and holds liquid bucket 5, and spray tank 16 is fixed
On 9 inner roof wall of case lid；By be provided with motor 2, ring-like frame 8, hold liquid bucket 5,14, No. two micro pumps 17 of No.1 micro pump can be real
Existing sample disposal, film-making, dyeing are once completed in an instrument, it can be achieved that same to sample LCT Thinprep pap test and cell DNA
The quick film-making of Shi Tongji and dyeing, accelerate film-making and dyeing time, that reduces doctor makes a definite diagnosis the time, enables the patient to obtain as early as possible
Effective treatment.
In use, first collected cell liquid is numbered, and by cell liquid shaker vibrate 45min (150 times/
Min), the titration processed cell liquid dropped on observation piece 18 is intracavitary, then observation piece 18 is placed sequentially on ring-like frame 8
Two groups of lens channels in be fixed.
Centrifugal treating：Motor 2 is opened, motor 2 drives rotation axis to make the speed rotation 5min for being centrifuged frame with 800r/min,
Complete the centrifugal treating to cell liquid.
Abandon supernatant processing：Cylinder 6 drives telescopic rod 7 increase ring-like frame 8, and dropper 13 is extended to and titrates intracavitary, unlatching
No. two micro pumps 17, No. two micro pumps 17 drive No.1 hoses 10 and No. two 11 pairs of hose titration it is intracavitary obtain the upper of sample
Clear liquid, which drains into, to be held in liquid bucket 5, and control cylinder 6 declines ring-like frame 8, completes to operate the abandoning supernatant of all samples.
Add ethyl alcohol：Cylinder 6 drives telescopic rod 7 increase ring-like frame 8, and it is intracavitary that dropper 13 is extended to titration, and it is micro- to open No.1
Amount pump 17, makes No.1 micro pump 17 draw the ethyl alcohol medical fluid in spray tank 16, by No.1 hose 10 and No. two hoses 11
Dropper 13 carries out 50% ethyl alcohol of injection to the titration chamber on corresponding observation piece 18, completes to all samples plus ethyl alcohol behaviour
Make；Secondary above-mentioned centrifugation step is repeated, secondary above-mentioned abandoning supernatant step is repeated.
It is fixed liquid：Cylinder 6 drives telescopic rod 7 increase ring-like frame 8, and dropper 13 is extended to and titrates intracavitary, unlatching No.1
Micro pump 14 makes No.1 micro pump 14 draw the formalin medicament in spray tank 16, passes through No.1 hose 10 and No. two hoses
Dropper 13 on 11 carries out 4% formalin buffer of injection to the titration chamber on corresponding observation piece 18, completes to institute
Have sample is fixed liquid operation.
It stirs and evenly mixs：Cylinder 6 drives telescopic rod 7 increase ring-like frame 8, and dropper 13 is extended to the intracavitary injection cell of titration and is delayed
Appropriate fliud flushing.Computerized control system is with CO8000 principle under 600nm light or Lamb wave is micro- measures the thin of cell suspension
Born of the same parents' density keeps the cell density of each sample tube roughly the same the pallium cell injection Cell Buffer of different densities, it is ensured that institute
The quality of cell patch processed is high.Motor 2 is opened, motor 2 drives the sample in observation piece 18 to be rotated, in natural sedimentation
10min, then forward and reverse rotate turntable 3 times, so that the cell being overlapped on cell patch vibration is fallen on underlying membrane on piece and are evenly distributed,
It obtains cell monolayer diaphragm and obtains cell suspension.
Dyeing：6 control ring type frame 8 of cylinder rises, and opens 14 pairs of titration 10% formalin of intracavitary administration of No.1 micro pump
Buffer；No. two micro pumps 17 are opened again surplus annex solution has been adsorbed to be discharged into and hold in liquid bucket 5, then inject the hydrolysis of 5mol/L hydrochloric acid
5min；Again surplus annex solution is adsorbed to be discharged into and held in liquid bucket 5, then has been injected into distilled water and carry out rinsing 10s；Repeat removal waste fluid.
Again to intracavitary injection staining reagent 2ml is titrated, 10min is dyed；Repeat removal waste fluid.Again to titration 95% ethyl alcohol 2ml of intracavitary injection
Carry out dehydration 10s；Repeat removal waste fluid.Dehydration 10s is carried out to titration chamber injection dehydrated alcohol 2ml again；Repeat removal waste fluid.Again to drop
Determine chamber injection dimethylbenzene 1ml, keeps 5s；Repeat removal waste fluid；Obtain the staining section of cell DNA.
Place is not described in detail for the utility model, is the well-known technique of those skilled in the art.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine, it is characterised in that：Including cabinet
(1), motor (2) are equipped in the middle part of the bottom wall in the cabinet (1), the output end of the motor (2) is coaxially connected with rotation axis, institute
Rotation axis top is stated in the middle part of partition (3) and end is coaxially connected with centrifugation frame (4), the roof of centrifugation frame (4) is equipped with
Cyclic annular lifting groove, the bottom wall two sides in the lifting groove are equipped with cylinder (6), and the output end of two cylinders (6) is equipped with
Telescopic rod (7), two telescopic rod (7) tops are fixedly connected with cyclic rings type frame (8), in the roof of the ring-like frame (8)
Equipped with ring-like lens channel, observation piece (18) is equipped in lens channel described in two groups, cabinet (1) top is equipped with case lid (9), institute
It states case lid (9) bottom end and is respectively equipped with No.1 cricoid cartilage (10) and No. two cricoid cartilages (11), the No.1 hose (10) and two
The side liquid feeding end of number hose (11) runs through case lid (9) bottom wall and communicates with the No.1 micro pump (14) being located in case lid, institute
The input terminal for stating No.1 micro pump (14) is communicated with No.1 fluid-through tube, is equipped with No.1 liquid-controlling valve (15) in the No.1 fluid-through tube,
The right end of the No.1 fluid-through tube is connected with spray tank, the other side drawing liquid of the No.1 hose (10) and No. two hoses (11)
End is communicated through case lid (9) bottom wall and with water main, and No. two liquid-controlling valves, the end of the water main are equipped in the water main
It being communicated with the input terminal of No. two micro pumps (17), the output ends of No. two micro pumps (17) is equipped with No. two fluid-through tubes, and described two
The end of number fluid-through tube is communicated through case lid (9) bottom wall and with leakage fluid dram (19).
2. a kind of LCT Thinprep pap test according to claim 1 and the quick film-making of cell DNA and dyeing all-in-one machine,
It is characterized in that：The No.1 hose (10) is fixedly connected by fixing buckle (12) with case lid (9) bottom wall with No. two hoses (11).
3. a kind of LCT Thinprep pap test according to claim 1 and the quick film-making of cell DNA and dyeing all-in-one machine,
It is characterized in that：The bottom end of the No.1 hose (10) and No. two hoses (11) is placed with multiple droppers (13), the dropper
(13) it is communicated respectively with No.1 hose (10), No. two hoses (11).
4. a kind of LCT Thinprep pap test according to claim 1 and the quick film-making of cell DNA and dyeing all-in-one machine,
It is characterized in that：Observation piece (18) roof is equipped with titration chamber.
5. a kind of LCT Thinprep pap test according to claim 1 and the quick film-making of cell DNA and dyeing all-in-one machine,
It is characterized in that：It is equipped with groove in the middle part of the roof of centrifugation frame (4), is equipped in the groove and holds liquid bucket (5).
6. a kind of LCT Thinprep pap test according to claim 1 and the quick film-making of cell DNA and dyeing all-in-one machine,
It is characterized in that：The spray tank (16) is fixed on case lid (9) inner roof wall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820649609.5U CN208125478U (en) | 2018-05-02 | 2018-05-02 | LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820649609.5U CN208125478U (en) | 2018-05-02 | 2018-05-02 | LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine |
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| Publication Number | Publication Date |
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| CN208125478U true CN208125478U (en) | 2018-11-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201820649609.5U Active CN208125478U (en) | 2018-05-02 | 2018-05-02 | LCT Thinprep pap test and the quick film-making of cell DNA and dyeing all-in-one machine |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113188872A (en) * | 2021-05-26 | 2021-07-30 | 北京诚智光辉科技有限公司 | Cell film-making dyeing all-in-one |
-
2018
- 2018-05-02 CN CN201820649609.5U patent/CN208125478U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113188872A (en) * | 2021-05-26 | 2021-07-30 | 北京诚智光辉科技有限公司 | Cell film-making dyeing all-in-one |
| CN113188872B (en) * | 2021-05-26 | 2023-01-31 | 北京诚智光辉科技有限公司 | Cell film-making dyeing all-in-one |
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