CN206020136U - A kind of integrated apparatus for preserving for cell and filtering - Google Patents
A kind of integrated apparatus for preserving for cell and filtering Download PDFInfo
- Publication number
- CN206020136U CN206020136U CN201620908005.9U CN201620908005U CN206020136U CN 206020136 U CN206020136 U CN 206020136U CN 201620908005 U CN201620908005 U CN 201620908005U CN 206020136 U CN206020136 U CN 206020136U
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- China
- Prior art keywords
- film carrier
- bottle
- carrier frame
- integrated apparatus
- film
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
This utility model provides a kind of integrated apparatus for preserving for cell and filtering, and the integrated apparatus include：Bottle, the bottle body bottom closing is in coniform；Film carrier frame, it is arranged in the bottle, and it is arranged in the bottle inwall, the film carrier top of the trellis arranges opening, the film carrier frame bottom lock, the film carrier frame includes that two film carrier portions, the two film carriers portion are respectively arranged at the side of the film carrier frame, and the opening, two film carrier portions and the space formed with film carrier frame bottom are film carrier chamber；Filter membrane, is fixed in the film carrier portion；Bottle cap, is covered on the bottle, and bottle and film carrier frame opening are closed simultaneously.Integrated apparatus described in the utility model can be used for preserving Cell sap first, also can cell specimen is vibrated and the pretreatment process that is centrifuged in, the large granular impurities such as the mucus in cell specimen are separated in the integrated apparatus in advance, so that follow-up film-making and Coloration experiment disposably can be automatically finished.
Description
Technical field
This utility model belongs to the sectioning cells and colouring art of liquid based cytology, more particularly to a kind of for cell preservation
And the integrated apparatus for filtering.
Background technology
Medically, doctor can pass through for diseases such as the observation of cell smear, Diagnosis of Female cervical cancers.Traditionally, carefully
Born of the same parents' film-making adopts manual method, i.e. doctor equably to apply collecting sample by hand on the glass sheet, through alcohol fixation, dyes, so
After examine under a microscope.However, Conventional slide hinders observation because there is the impurity such as substantial amounts of mucus, rate of missed diagnosis is higher, now
Progressively replaced by liquid based cytology.
One of major advantage of liquid based cytology is, before film-making, by technical finesse, will be miscellaneous for mucus in Cell sap etc.
Matter is separated from Cell sap, so that sectioning cells are clear, it is easy to observe.The technology for separating mucus has multiple, wherein makes
With the most extensively and the cheapest method of cost is Filtration.Existing filtering technique is：A filter membrane is installed in preparation bin,
The cell-preservation liquid being centrifuged is transferred in preparation bin, cell carries out sedimentation film-making on microscope slide by fenestra stream, and glues
Liquid has been left on filter membrane.It is a kind of polycrystalline substance schematic diagram of preparation bin as shown in Figure 1.But, filter membrane in preparation bin
Presence, hamper the Coloration experiment of next step, it is impossible to add dyeing liquor by the tubular opening of preparation bin on microscope slide
Cell dyeing, and need to disassemble preparation bin, take out microscope slide and go to dye, so that whole experiment process can not be by instrument
Once complete, make whole experiment process become loaded down with trivial details, and excessive manual intervention in experimentation, increased the probability of error.
Content of the invention
For the problems referred to above of the prior art, this utility model provides a kind of one for preserving for cell and filtering
Makeup is put, and the integrated apparatus can be used to preserve Cell sap, while, it is also possible to cell specimen is being vibrated and is being centrifuged
In pretreatment process, just mucus in cell specimen etc. is separated in the integrated apparatus in advance, so that after
Continuous film-making and Coloration experiment once can be completed.
In order to achieve the above object, this utility model is adopted the following technical scheme that：A kind of for cell preserve and filter
Integrated apparatus, including：
Bottle, the bottle body bottom closing is in coniform；
Film carrier frame, is arranged in the bottle, and is arranged in the bottle inwall, and the film carrier top of the trellis arranges opening,
Bottom lock；The film carrier frame also includes two film carrier portions, and the two film carriers portion is respectively arranged at the side of the film carrier frame, described
The opening of film carrier frame, two film carrier portions and the space formed with the bottom of film carrier frame are film carrier chamber；
Filter membrane, is fixed in the film carrier portion；
Bottle cap, is covered on the bottle, with to bottle and film carrier frame closure of openings.
As further preferred, position limiting structure is provided with the film carrier frame outer surface, is arranged in institute for film carrier frame
State bottle inwall.
As further preferred, the limited block that the position limiting structure is extended for film carrier frame outer surface.
As further, preferably, the limited block is 4, is evenly distributed on around film carrier frame outer surface.
Used as further preferably, the limited block is arranged on the outer surface of the film carrier frame lower end, and will be carried using which
Film frame is shelved at the maximum gauge of coniform bottom, i.e., the upper end of coniform bottom.
Preferably the film carrier top of the trellis outer surface is provided with supporting construction as further, for the spacing knot
Structure ensures that film carrier frame is concentric with bottle cap together and places.
As further, preferably, the supporting construction is 4 support blocks, is uniformly arranged on the film carrier top of the trellis appearance
Around face.
Used as further preferably, the length of the support block is 1-5mm, makes the cylindrical and bottle inwall at film carrier frame opening
Keep enough gaps.
Used as further preferably, the bottle cap includes that lid and lateral margin, the lid inner surface setting have internal ring to swell；
The upper end open of the bottle is sealed in the ditch that formed between the lateral margin and internal ring protuberance；Stretch into the film carrier frame upper end
The inner side of internal ring protuberance.
As further, preferably, the film carrier frame upper end is provided with projection, for stretching into the inner side of internal ring protuberance, described
Raised height is highly consistent with what internal ring swelled；The convex outer surface is cased with sealing ring.
Used as further preferably, the internal ring bump height is 1-10mm.
As further preferred, the diameter slightly smaller than bottle internal diameter of the film carrier frame.
As further, preferably, the top diameter and bottle inwall of the film carrier frame are maintained between 0.3mm-0.5mm
Gap.
As further, preferably, the film carrier top of the trellis is rounded, and bottom is rectangle.
As further, preferably, the mesh of the filter membrane is in 50-200 mesh.
As further, preferably, the bottle upper body is cylindrical.
Preferably the coniform bottom is additionally provided with outer housing as further, the outer housing upper end and bottle upper body
Connection, the outer housing lower end are that plane is used for integrated apparatus placement conveniently, to avoid coniform bottom injustice from being difficult to put
The problem that puts.
The beneficial effects of the utility model are as follows：
1st, this utility model has the functional structure of cell-preservation liquid bottle first, and its coniform bottom can be used for centrifugation
Step, and film carrier frame is loaded with inside this utility model integrated apparatus, vibrating the integrated apparatus can make addition
Cell in membrane cavity is transferred in the liquid outside membrane cavity by filter membrane, is centrifuged this utility model integrated apparatus afterwards, is beaten
Corkage lid, directly outwells the liquid and film carrier frame in bottle, it is possible to collects the cell sample through filtering, is the system of next step
Piece dyeing realizes that automatization lays a good foundation.
2nd, the filter membrane of this utility model film carrier frame is arranged on side rather than bottom surface, can increase filter area, and
Compared with bottom surface is arranged on, stop area when cell centrifugation is precipitated is reduced.
3rd, the setting and its cooperation with bottle cap of this utility model film carrier frame structure, it is ensured that be added to thin in load die cavity
Born of the same parents' sample will not leak into bottom of bottle by sidewall spacers, and have to pass through the drainage screen for carrying die cavity, it is ensured that the effect of filtration.
4th, this utility model when using, while bottle opening is sealed, tie also by fixing seal by the film carrier frame
Structure is simple and practical, and the diameter of the film carrier frame is less than bottle internal diameter so as to can pour out from bottle upper opening, so as to enter
The follow-up film-making dying operation of row.
Description of the drawings
Structural representations of the Fig. 1 for prior art preparation bin.
Structural representations (do not illustrate drainage screen) of the Fig. 2 for the embodiment of the present application integrated apparatus.
Fig. 3 is the structural representation of film carrier frame when not adding drainage screen.
Fig. 4 be plus during drainage screen film carrier frame structural representation.
Structural representations of the Fig. 5 for bottle cap inner surface.
In accompanying drawing, labelling is described as follows：1- bottles, 11- upper bodies, 12- bottoms, 13- outer housings, 2- film carrier framves, 21- are opened
Mouth, 22- film carriers portion, 23- film carrier framves bottom, 24- film carriers chamber, 25- limited blocks, 26- support blocks, 27- projections, 3- filter membranes, 4-
Bottle cap, 41- lids, 42- lateral margins, 43- internal ring protuberance, 44- ditches.
Specific embodiment
, by providing a kind of integrated apparatus for preserving for cell and filtering, the integrated apparatus can for this utility model
For preserving Cell sap, while, it is also possible to cell specimen is vibrated and the pretreatment process that is centrifuged in, just in advance in institute
State in integrated apparatus and mucus in cell specimen etc. is separated, so that follow-up film-making and Coloration experiment can be once
Complete.
In order to be better understood from above-mentioned technical proposal, below in conjunction with Figure of description and specific embodiment to upper
State technical scheme to be described in detail.
The embodiment of the present application is used for the integrated apparatus that cell is preserved and filtered, including：
Bottle, the bottle body bottom closing is in coniform；
Film carrier frame, is arranged in the bottle, and is arranged in the bottle inwall, arranges opening, the film carrier at the top of which
Frame includes that two film carrier portions, the two film carriers portion are respectively arranged at the side of the film carrier frame, the opening at the top, two film carrier portions
And the space formed with film carrier frame bottom is film carrier chamber；Herein side be defined as with through bottle height centerline parallel or
Face at an angle, the angle are less than 90 degree, the film carrier frame bottom lock；
Filter membrane, is fixed in the film carrier portion；Wherein, cell sample liquid is put into the load via the film carrier frame opening
Membrane cavity, during through vibration, centrifugally operated, sample liquid can be preserved to the coniform bottom of bottle after being filtered by the filter membrane.
Bottle cap, is covered on the bottle, and bottle and film carrier frame opening are closed simultaneously；
Select to arrange film carrier portion on the larger two sides of the film carrier frame surface area, to improve filtration flow effect.
Film carrier frame lower end is advisable with just accommodating cytology brush in flat as thin as possible so as to block minimum to bottom of bottle.
Position limiting structure is provided with the film carrier frame outer surface, the bottle inwall is arranged in for film carrier frame.
The position limiting structure is the limited block that film carrier frame outer surface is extended.
The limited block is 4, is evenly distributed on around film carrier frame outer surface.
The limited block is arranged on the outer surface of the film carrier frame lower end, and film carrier frame is shelved on coniform bottom using which
At the maximum gauge in portion, i.e., the upper end of coniform bottom.
The film carrier top of the trellis outer surface is provided with supporting construction, and which ensure that film carrier frame and bottle cap together with position limiting structure
Place with one heart.
The supporting construction is 4 support blocks, is uniformly arranged on around the film carrier top of the trellis outer surface.
The bottle upper body is cylindrical.
The coniform bottom is additionally provided with outer housing, and the outer housing upper end is connected with bottle upper body, the outer housing lower end
It is that plane is used for integrated apparatus placement conveniently, to avoid coniform bottom injustice from being difficult to the problem that places.
The bottle cap includes that lid and lateral margin, the lid inner surface setting have internal ring to swell；Open the upper end of the bottle
Mouth is sealed in the ditch that formed between the lateral margin and internal ring protuberance；The inner side of internal ring protuberance is stretched in the film carrier frame upper end,
While bottle opening is sealed, the film carrier frame is also fixed by sealing.The inner side is defined as towards the center of circle or central point
Direction.Said structure, and must be through for ensureing that carrying the cell sample in die cavity will not leak into bottom of bottle by sidewall spacers
The drainage screen of overload die cavity.
The film carrier frame upper end is provided with projection, for stretching into the inner side of internal ring protuberance, the raised height and internal ring
Swelled is highly consistent.
The convex outer surface is cased with sealing ring.
The internal ring bump height is 1-10mm.
The diameter of the film carrier frame is slightly smaller than bottle internal diameter so as to can pour out from bottle upper opening.
State that film carrier top of the trellis is rounded, bottom is rectangle.
The top diameter of the film carrier frame maintains the gap of 0.3mm-0.5mm with bottle inwall so that film carrier frame is easy
Pour out from bottle upper opening.
The mesh of the filter membrane is in 50-200 mesh.
Embodiment 1
As in Figure 2-4, the embodiment of the present application is used for the integrated apparatus that cell is preserved and filtered, the integrated apparatus
Including：
Bottle 1, the bottle upper body 11 are cylindrical, and the closing of the bottle body bottom 12 is in coniform；Described coniform
Bottom is additionally provided with outer housing 13, and 13 upper end of the outer housing is connected with 11 upper body of bottle, and 13 lower end of the outer housing is that plane is used for
It is convenient that the integrated apparatus are placed, to avoid coniform bottom injustice from being difficult to the problem that places.
Film carrier frame 2, is arranged in the bottle 1, and is arranged in the bottle inwall, arranges opening 21 at the top of which, described
Film carrier frame includes that two film carrier portions 22, the two film carriers portion 22 are respectively arranged at the side of the film carrier frame, the opening at the top
21st, two film carrier portions 22 and the space formed with film carrier frame bottom 23 are film carrier chamber 24；Side is defined as and runs through bottle herein
Close in the centerline parallel of height or the face being slightly tilted, the film carrier frame bottom 23；Rounded at the top of the film carrier frame 2, under
Portion is rectangle.The diameter of the film carrier frame 2 is slightly smaller than 1 internal diameter of bottle so as to can pour out from 1 upper opening of bottle.The load
The top diameter of film frame 2 maintains the gap of 0.4mm with 1 inwall of bottle so that film carrier frame is easily poured out from bottle upper opening.
Limited block 25 is provided with the film carrier frame outer surface, the bottle inwall is arranged in for film carrier frame.The limited block 25 is 4
Individual, it is evenly distributed on around film carrier frame outer surface.The limited block 25 is arranged on the outer surface of the film carrier frame lower end, and profit
Film carrier frame is shelved at the maximum gauge of coniform bottom with which, i.e., the upper end of coniform bottom.Outside the film carrier top of the trellis
Surface is provided with supporting construction, and which ensure that together with position limiting structure film carrier frame is concentric with bottle cap and places.The supporting construction is 4
Individual support block 26, is uniformly arranged on around the film carrier top of the trellis outer surface.The length of support block is 4mm, makes film carrier frame opening
The cylindrical and bottle inwall at place keeps enough gaps so that when pouring out film carrier frame from bottle upper opening, air is easily accessible bottle
Internal portion.
Filter membrane 3, is fixed in the film carrier portion 22；Wherein, sample liquid is put into described via the film carrier frame opening 21
Film carrier chamber 24, during through vibration, centrifugally operated, sample liquid can arrive the coniform bottom of bottle by the filter membrane 3 after being filtered
12 preserve.The mesh of the filter membrane is in 100 mesh.Select to arrange on the larger two sides of the film carrier frame surface area and carry
Film portion 22, improves filtration flow effect to increase filtration membrane area.
Bottle cap 4, is covered on the bottle 1, and bottle 1 and film carrier frame opening 21 are closed simultaneously；The bottle cap 4 includes covering
There is internal ring protuberance 43 body 41 and lateral margin 42,41 inner surface setting of the lid；The upper end open of the bottle is sealed in described
In the ditch 44 formed between lateral margin 42 and internal ring protuberance 43；The inner side of internal ring protuberance 43 is stretched in 2 upper end of film carrier frame, in bottle
While opening is sealed, the film carrier frame 2 is also fixed by sealing.Said structure carries the cell sample in die cavity for guarantee
Bottom of bottle will not be leaked into by sidewall spacers, and have to pass through the drainage screen 3 in film carrier chamber 24.2 upper end of film carrier frame is provided with
Raised 27, for stretching into the inner side of internal ring protuberance 43, raised 27 height is highly consistent with internal ring protuberance 43.Described interior
Ring bump height is 5mm.Raised 27 outer surface is cased with sealing ring.
Technical scheme in above-mentioned the embodiment of the present application, at least has the following technical effect that or advantage：
1st, this utility model has the functional structure of cell-preservation liquid bottle first, and its coniform bottom can be used for centrifugation
Step, and film carrier frame is loaded with inside this utility model integrated apparatus, vibrating the integrated apparatus can make addition
Cell in membrane cavity is transferred in the liquid outside membrane cavity by filter membrane, is centrifuged this utility model integrated apparatus afterwards, is beaten
Corkage lid, directly outwells the liquid and film carrier frame in bottle, it is possible to collects the cell sample through filtering, is the system of next step
Piece dyeing realizes that automatization lays a good foundation.
2nd, the filter membrane of this utility model film carrier frame is arranged on side rather than bottom surface, can increase filter area, and
Compared with bottom surface is arranged on, the stop area of cell settlement when reducing centrifugation.
3rd, the setting and its cooperation with bottle cap of this utility model film carrier frame structure, it is ensured that be added to the thin of film carrier intracavity
Born of the same parents' sample will not leak into bottom of bottle by sidewall spacers, and have to pass through the drainage screen in film carrier chamber, it is ensured that the effect of filtration.
4th, this utility model when using, while bottle opening is sealed, also fixed by sealing by the film carrier frame, knot
Structure is simple and practical, and the diameter of the film carrier frame is slightly smaller than bottle internal diameter so as to can pour out from bottle upper opening, so as to can
Carry out follow-up dying operation.
Although having been described for preferred embodiment of the present utility model, those skilled in the art once know substantially
Creative concept, then can make other change and modification to these embodiments.So, claims are intended to be construed to wrap
Include preferred embodiment and fall into the had altered of this utility model scope and change.Obviously, those skilled in the art can be with
Various changes and modification is carried out to this utility model without deviating from spirit and scope of the present utility model.So, if this practicality
These new modifications and modification belong within the scope of this utility model claim and its equivalent technologies, then this utility model
It is also intended to comprising these changes and modification.
Claims (10)
1. a kind of for cell preserve and filter integrated apparatus, it is characterised in that：The integrated apparatus include：
Bottle, the bottle body bottom closing is in coniform；
Film carrier frame, is arranged in the bottle, and is arranged in the bottle inwall, and the film carrier top of the trellis arranges opening, bottom
Closing；The film carrier frame also includes that two film carrier portions, the two film carriers portion are respectively arranged at the side of the film carrier frame, the film carrier
The opening of frame, two film carrier portions and the space formed with the bottom of film carrier frame are film carrier chamber；
Filter membrane, is fixed in the film carrier portion；
Bottle cap, is covered on the bottle, to close to bottle and film carrier frame opening.
2. integrated apparatus according to claim 1, it is characterised in that：Limited location knot is set on the film carrier frame outer surface
Structure, is arranged in the bottle inwall for film carrier frame.
3. integrated apparatus according to claim 2, it is characterised in that：The position limiting structure is extended for film carrier frame outer surface
Limited block.
4. integrated apparatus according to claim 3, it is characterised in that：The limited block is 4, is evenly distributed on film carrier
Around frame outer surface.
5. integrated apparatus according to claim 2, it is characterised in that：The film carrier top of the trellis outer surface is provided with support
Structure, is placed for being ensured that together with the position limiting structure film carrier frame and bottle cap are concentric.
6. integrated apparatus according to claim 5, it is characterised in that：The supporting construction is 4 support blocks, uniformly sets
Put around the film carrier top of the trellis outer surface.
7. integrated apparatus according to claim 1, it is characterised in that：The bottle cap includes lid and lateral margin, the lid
Surface is provided with internal ring protuberance in vivo；The upper end open of the bottle is sealed in formation between the lateral margin and internal ring protuberance
In ditch；Stretch into the inner side of internal ring protuberance in the film carrier frame upper end.
8. integrated apparatus according to claim 7, it is characterised in that：The film carrier frame upper end is provided with projection, is used for
The inner side of internal ring protuberance is stretched into, the raised height is highly consistent with what internal ring swelled；The convex outer surface is cased with sealing
Circle.
9. integrated apparatus according to claim 1, it is characterised in that：The diameter of the film carrier frame is less than bottle internal diameter.
10. integrated apparatus according to claim 1 or 9, it is characterised in that：The film carrier top of the trellis is rounded, bottom
It is rectangle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620908005.9U CN206020136U (en) | 2016-08-19 | 2016-08-19 | A kind of integrated apparatus for preserving for cell and filtering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620908005.9U CN206020136U (en) | 2016-08-19 | 2016-08-19 | A kind of integrated apparatus for preserving for cell and filtering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206020136U true CN206020136U (en) | 2017-03-15 |
Family
ID=58250214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620908005.9U Expired - Fee Related CN206020136U (en) | 2016-08-19 | 2016-08-19 | A kind of integrated apparatus for preserving for cell and filtering |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206020136U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106996891A (en) * | 2017-05-22 | 2017-08-01 | 上海市第人民医院 | It is a kind of to be used for the Dyeing pipe and colouring method of not attached cell immunostaining |
| CN113145191A (en) * | 2021-04-28 | 2021-07-23 | 广州复安生物科技有限公司 | Sample bottle, sample placing device, slice-making dyeing machine and slice-making dyeing method |
-
2016
- 2016-08-19 CN CN201620908005.9U patent/CN206020136U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106996891A (en) * | 2017-05-22 | 2017-08-01 | 上海市第人民医院 | It is a kind of to be used for the Dyeing pipe and colouring method of not attached cell immunostaining |
| CN106996891B (en) * | 2017-05-22 | 2023-06-13 | 上海市第一人民医院 | Staining tube and staining method for non-adherent cell immunostaining |
| CN113145191A (en) * | 2021-04-28 | 2021-07-23 | 广州复安生物科技有限公司 | Sample bottle, sample placing device, slice-making dyeing machine and slice-making dyeing method |
| CN113145191B (en) * | 2021-04-28 | 2024-02-20 | 广州复安生物科技有限公司 | Sample bottle, sample placement device, slide-making dyeing machine and slide-making dyeing method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170315 Termination date: 20190819 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |