CN113145191A - Sample bottle, sample placing device, slice-making dyeing machine and slice-making dyeing method - Google Patents
Sample bottle, sample placing device, slice-making dyeing machine and slice-making dyeing method Download PDFInfo
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- CN113145191A CN113145191A CN202110470346.8A CN202110470346A CN113145191A CN 113145191 A CN113145191 A CN 113145191A CN 202110470346 A CN202110470346 A CN 202110470346A CN 113145191 A CN113145191 A CN 113145191A
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- 238000004043 dyeing Methods 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims description 56
- 238000000605 extraction Methods 0.000 claims description 14
- 238000010186 staining Methods 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 7
- 239000002699 waste material Substances 0.000 claims description 7
- 239000012535 impurity Substances 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 10
- 238000012546 transfer Methods 0.000 abstract description 7
- 210000004027 cell Anatomy 0.000 description 21
- 239000000243 solution Substances 0.000 description 16
- 238000009434 installation Methods 0.000 description 14
- 238000011084 recovery Methods 0.000 description 12
- 239000010902 straw Substances 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 238000004064 recycling Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000001575 pathological effect Effects 0.000 description 4
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- 230000002380 cytological effect Effects 0.000 description 3
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- 230000008569 process Effects 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000010827 pathological analysis Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
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- 239000000284 extract Substances 0.000 description 1
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- 239000011521 glass Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
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- 210000003802 sputum Anatomy 0.000 description 1
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Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The invention discloses a sample bottle, a sample placing device, a slice-making dyeing machine and a slice-making dyeing method, wherein the sample bottle comprises a bottle body, the bottle body is provided with an upper opening, a filter screen is arranged in the bottle body, and the filter screen divides the bottle body into a first space and a second space; the side of the upper opening opposite to the first space is a sample placing side, and the side of the upper opening opposite to the second space is a sample extracting side. The invention can conveniently realize the transfer and enrichment of the sample and effectively improve the detection efficiency.
Description
Technical Field
The invention belongs to the field of medical detection, and particularly relates to a sample bottle, a sample placing device, a slice-making dyeing machine and a slice-making dyeing method.
Background
The steps of the liquid-based cytological pathological examination are as follows: sampling, preparing slices, staining and observing and diagnosing. For each step of the liquid-based cytological pathological examination, the corresponding in vitro diagnostic equipment for realizing the liquid-based cytological pathological examination comprises a sample transfer machine, a centrifugal machine, a liquid-based oscillator, a slice production and dyeing machine and the like. Wherein, the slide-making and dyeing machine is commonly used for making and dyeing human cells or bacteria samples before pathological analysis. The method removes interference components in a specimen through a special reagent, and performs batch slide and staining on cells to prepare a thin-layer cell sheet for pathological diagnosis. Is suitable for the pathological examination of cervical cells, sputum/alveolar lavage fluid, urine cells, serosal cavity cells, needle-aspiration cells and other liquid-based cells.
In the process of implementing the invention, the inventor finds that at least the following problems exist in the prior art:
the structural design of the existing film-making dyeing machine and the matched sample container is unreasonable, the sample container has a single function, and the transfer and enrichment of the sample cannot be conveniently realized in the detection process, so that the detection efficiency is influenced.
Disclosure of Invention
The invention aims to provide a sample bottle, a sample placing device, a slice-making dyeing machine and a slice-making dyeing method.
The technical scheme is as follows:
the invention provides a sample bottle, which comprises a bottle body, wherein the bottle body is provided with an upper opening, a filter screen is arranged in the bottle body, and the filter screen divides the bottle body into a first space and a second space; the side of the upper opening opposite to the first space is a sample placing side, and the side of the upper opening opposite to the second space is a sample extracting side.
In one embodiment, the bottle body comprises an inner bottle and an outer bottle, the inner bottle is arranged in the outer bottle and is positioned on one side of the outer bottle, the first space is formed in the inner bottle, the second space is formed between the inner bottle and the outer bottle, and the filter screen is arranged on the inner bottle.
In one embodiment, the filter screen is disposed at the bottom of the inner bottle and on the opposite side of the second space.
In one embodiment, the bottom of the inner bottle is lower at the side close to the second space, and is higher at the side far away from the second space, so as to form a second inclined bottom in an inclined shape, a first inclined bottom is formed at the bottom of the outer bottle, the inclined directions of the first inclined bottom and the second inclined bottom are the same, a lower opening is arranged on the second inclined bottom, and the filter screen is arranged at the lower opening.
In one embodiment, a first anti-reverse-protruding part is arranged on the inner wall of the outer bottle.
In one embodiment, the bottle further comprises a bottle cap, the bottle cap is matched with the upper opening, the radial inner side of the upper surface of the bottle cap is locally concave to form an inner groove, and the inner groove is at least opposite to the second space of the bottle body.
The invention also provides a sample placing device, which comprises a base and a sample frame, wherein a second mounting groove is formed in the base, the sample frame is placed in the second mounting groove and is in sliding fit with the second mounting groove, a plurality of sample placing positions for placing the sample bottles of claim 1 are arranged on the sample frame, the sample bottles are placed in the sample placing positions, a shielding piece is arranged on the base, the shielding piece is provided with a shielding part and a position yielding part, the shielding part corresponds to the sample bottles, and the position yielding part corresponds to the sample extracting side.
In one embodiment, the shielding member is a plurality of blocking strips arranged on the base, the blocking strips are arranged in parallel, strip holes are formed among the blocking strips, the blocking strips form the shielding portion, and the strip holes form the relief portion.
In one embodiment, the width of the strip aperture is less than the width of the sample vial.
In one embodiment, a second locking member is disposed between the sample rack and the second mounting groove.
The invention also provides a slice making dyeing machine, wherein a frame, a pipe frame, a dye solution frame, a slice making dyeing plate and the sample placing device are also arranged on the machine base; the frame is provided with a mechanical arm, and the mechanical arm is provided with a suction head facing to the pipe frame, the sample frame, the dyeing liquid frame and the slice-making dyeing plate.
The invention also provides a tablet-making and dyeing method, which comprises the following steps:
placing the sample into a sample-placing side of the sample bottle;
repeatedly sucking liquid through the suction head, driving the liquid to form liquid flows on two sides of the filter screen, and screening target cells of the sample by the liquid flows to enter a sample extraction side;
repeatedly filtering to enrich the target cells of the sample to the sample extraction side;
sucking the liquid and the target cells on the sample extraction side through a suction pipe, and transferring to a slice preparation staining plate;
staining the target cells;
the stained specimens on the slide staining plate were examined.
In one embodiment, in the above step, when the liquid is extracted through the suction head, the liquid flow drives the target cells on the sample introduction side to the sample extraction side; when the liquid is discharged through the suction head, the liquid flow washes away the sample impurities blocked on the filter screen.
In one embodiment, in the foregoing steps, before the test, the pump body pumps the cleaning liquid in the liquid bottle to empty the air in the suction head and the suction tube, the excess liquid is stored in the waste liquid tank, and after the test, the pump body pumps the liquid in the waste liquid tank into the suction head and the suction tube for cleaning the suction head and the suction tube.
In one embodiment, in the above step, the robot moves to move the suction head downward and press the pipette on the rack into the suction head.
The technical scheme provided by the invention has the following advantages and effects:
the sample bottle adopts a separation type structure and is provided with a first space and a second space, one side of an upper opening of the sample bottle, which is opposite to the first space, forms a sample placing side, and one side of the upper opening, which is opposite to the second space, forms a sample extracting side; when examining, put into the side from the sample of sample bottle and put into the sample, draw the side from the sample and insert the straw, and draw repeatedly through the reagent of straw to sample bottle, make the target cell separate from the sample, reuse straw extraction and carry out the enrichment with the sample, shift, the transfer and the enrichment of realization sample that can be convenient through this structure, whole in-process does not need the manual work to shift and link up, avoid the sample pollution among the transfer process, the structure is simple relatively, it is more convenient to operate.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles and effects of the invention.
Unless otherwise specified or defined, the same reference numerals in different figures refer to the same or similar features, and different reference numerals may be used for the same or similar features.
FIG. 1 is a block diagram of the whole of the slide-dyeing machine in an embodiment of the present invention;
FIG. 2 is a side view of the pipe rack mounting structure in an embodiment of the present invention;
FIG. 3 is a transverse cross-sectional view of the pipe rack mounting structure in an embodiment of the present invention;
FIG. 4 is a longitudinal cross-sectional view of the pipe rack mounting structure in an embodiment of the present invention;
FIG. 5 is a top view of the pipe rack mounting structure in an embodiment of the present invention;
FIG. 6 is a top view of the pipe rack mounting structure in an embodiment of the present invention;
FIG. 7 is a longitudinal cross-sectional view of the sample holder in an embodiment of the present invention;
FIG. 8 is a cross-sectional view of the sample rack and sample bottle in an embodiment of the present invention;
FIG. 9 is a cross-sectional view of the sample rack, sample bottle and pipette of an embodiment of the present invention;
FIG. 10 is a cross-sectional view of the sample vial in an embodiment of the present invention;
FIG. 11 is an exploded view of the sample vial in accordance with an embodiment of the present invention;
FIG. 12 is a block diagram of the outer bottle in an embodiment of the present invention;
FIG. 13 is a block diagram of the inner bottle in an embodiment of the present invention;
FIG. 14 is a block diagram of the recovery tank in an embodiment of the present invention;
description of reference numerals:
10. a machine base, 11, a machine frame, 111, a translation bracket, 112, a mechanical arm, 12, a first installation groove, 121, a longitudinal sliding groove, 13, a second installation groove, 14, a third installation groove, 15, a recovery box, 151, a handle, 152, a recovery cavity, 16 and a recovery opening,
20. a pipe frame 201, a first positioning hole 202, a guide projection 21, a first locking member 211, a guide inclined surface 22, a straw placing position 23, a first position sensing element 24, a first lifting lug 251, a bottom plate 252, a connecting plate 253, a top plate,
30. a sample rack 301, a sample placing position 302, a magnetic block 303, a second position sensing element 31, a second lifting lug 32, a first positioning part,
40. a sample bottle 41, an outer bottle 411, a first anti-reverse-protrusion part 412, a first inclined bottom 413, an external thread 42, an inner bottle 421, a filter net 422, a second anti-reverse-protrusion part 423, a second inclined bottom 431, a first space 432, a second space 441, a sample placing side 442, a sample extracting side 45, a second positioning part 46, a bottle cap 461, an inner groove,
71. a dye solution rack 711, a dye solution placing position 72, a slice preparation dyeing plate 721 and a slide placing position,
81. a barrier strip 82, a strip hole 83, a tube-removing sheet 831 and a tube-removing notch,
93. suction head 94, liquid bottle 95, waste liquid groove 96 and suction tube.
Detailed Description
In order to facilitate an understanding of the invention, specific embodiments thereof will be described in more detail below with reference to the accompanying drawings.
Unless specifically stated or otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In the case of a real-world scenario incorporating the technical solution of the present invention, all technical and scientific terms used herein may also have meanings corresponding to the purpose of achieving the technical solution of the present invention.
As used herein, unless otherwise specified or defined, "first" and "second" … are used merely for name differentiation and do not denote any particular quantity or order.
As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items, unless specified or otherwise defined.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly secured to the other element or intervening elements may also be present; when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present; when an element is referred to as being "mounted on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "on" another element, it can be directly on the other element or intervening elements may also be present.
As used herein, unless otherwise specified or defined, the terms "comprises," "comprising," and "comprising" are used interchangeably to refer to the term "comprising," and are used interchangeably herein.
It is needless to say that technical contents or technical features which are contrary to the object of the present invention or clearly contradicted by the object of the present invention should be excluded.
As shown in fig. 1 to 14, the slice-making and dyeing machine includes a machine base 10, a frame 11 is provided on the machine base 10, a translation bracket 111 is provided on the frame 11, a mechanical arm 112 is provided on the translation bracket 111, and a suction head 93 capable of moving up and down is mounted on the mechanical arm 112. The frame 10 is provided with a dye solution rack 71, a tube rack 20, a slide preparation staining plate 72 and two sample racks 30, the sample racks 30 are provided with a plurality of sample placing positions 301, the dye solution rack 71 is provided with a plurality of dye solution placing positions 711, and the slide preparation staining plate 72 is provided with a plurality of slide placing positions 721; the suction head 93 is movable up and down and left and right with respect to the rack 11, and the suction head 93 is directed toward the tube rack 20, the sample rack 30, the dye solution rack 71, or the slide dyeing plate 72.
But pipe support 20, sample frame 30 all adopt pull formula structure: a first mounting groove 12, a second mounting groove 13 and a third mounting groove 14 are formed on the base 10.
Please focus on and refer to fig. 2 to 5, the pipe rack 20 is installed at the first installation groove 12 and is in sliding fit with the first installation groove 12, the pipe rack 20 can be movably drawn out relative to the first installation groove 12, a first locking member 21 is arranged between the pipe rack 20 and the first installation groove 12, a plurality of through holes are arranged on the pipe rack 20 to form a plurality of straw placement positions 22, the pipe rack 20 is installed on the first installation groove 12 in a sliding fit manner, when in use, the pipe rack 20 can be pulled out of the first installation groove 12 in a pulling manner, the pipe rack 20 can also be pushed into the first installation groove 12, the pipe rack 20 can be conveniently drawn out, so as to conveniently place and take out the straws 96, or perform operations such as maintenance and cleaning, and the use is very convenient.
In this embodiment, an elastic member (not shown) is disposed below the first locking member 21, the outer side of the first locking member 21 is lower than the inner side, an inclined guiding-in slope 211 is formed at the upper portion of the first locking member 21, the first locking member 21 is located at the outer end of the first mounting groove 12 and corresponds to the outer end of the pipe frame 20, and a first positioning hole 201 corresponding to the first locking member 21 is disposed at the bottom of the pipe frame 20. When the pipe frame 20 is slid in, the pipe frame 20 can slide in along the lead-in slope 211 and lock the outer end of the pipe frame 20 by the first locking member 21, and also can lock at the first positioning hole 201 of the pipe frame 20 by the first locking member 21.
A first position sensing element 23 is provided on the first installation groove 12, the first position sensing element 23 facing the pipe frame 20. The first position sensor 23 can sense the sliding position of the pipe carrier 20 and give an alarm when the position of the pipe carrier 20 is not slid in place. The first position sensing element 23 may be a light sensing element, a magnetic sensing element, or other type of sensing element.
The two ends of the pipe support 20 are both provided with first lifting lugs 24, and the first lifting lugs 24 are higher than the heights of the table tops at the two ends of the first mounting groove 12. The pipe frame 20 can be pushed, pulled, moved and the like through the first lifting lug 24, and convenience of operation is improved. In order to avoid interference and influence on operation, the first lifting lug 24 is higher than the heights of the table tops at two ends of the first mounting groove 12.
Please refer to fig. 3, the pipe rack 20 includes a bottom plate 251, a connecting plate 252, and a top plate 253, the bottom plate 251 is connected to the top plate 253 through the connecting plate 252, the top plate 253 is provided with a plurality of through holes to form a plurality of straw placement positions 22, the width of the bottom plate 251 is greater than that of the top plate 253, guiding protrusions 202 are formed on two sides of the bottom plate 251, longitudinal sliding grooves 121 are formed on two sides of the first mounting groove 12, and the guiding protrusions 202 are inserted into the sliding grooves and are in sliding fit with the sliding grooves. The guide protrusions 202 are formed on both sides of the bottom plate 251 by connecting the bottom plate 251 and the top plate 253 with the connection plate 252, which is simple in structure and easy to install.
Please refer to fig. 6 to 13, two sample racks 30 are provided, two sample racks 30 are disposed in the second mounting groove 13 and are in sliding fit with the second mounting groove 13, the second mounting groove 13 is disposed on the base 10 of the slice-making and dyeing machine of the present embodiment, the sample racks 30 are disposed in the second mounting groove 13 and are in sliding fit with the second mounting groove 13, when in use, the sample racks 30 can be pulled out of the second mounting groove 13 by pulling, and the sample racks 30 can also be pushed into the second mounting groove 13, so as to perform operations such as batch loading of samples, maintenance and cleaning of the sample racks 30, and the use is very convenient, the two sample racks 30 are two, and a user can use two sample racks 30 at the same time or in a staggered manner according to a detection requirement, so as to improve the working efficiency.
The sample rack 30 and the second mounting groove 13 are provided with a second locking member therebetween, in this embodiment, the base 10 is provided with a magnetic attraction block 302, and a position of the sample rack 30 corresponding to the magnetic attraction block 302 is made of a magnetic attraction material, so that it can be understood that the magnetic attraction material can be a local part, i.e., the magnetic attraction block or an iron block is mounted on the local part, and the whole sample rack 30 can also be processed by using an iron material. The magnetic block 302 forms a second locking member, and when the sample rack 30 slides in place, the position of the sample rack 30 is locked by the magnetic block 302, so that the sample rack 30 is prevented from falling off; a second position sensing member 303 is provided on the second mounting groove 13, and the second position sensing member 303 faces the sample rack 30. The sliding position of the sample holder 30 can be sensed by the second position sensing element 303, and an alarm is given when the position of the sample holder 30 is not slid in place. The second position sensing element 303 may be a photo sensing element, a magnetic sensing element, or other type of sensing element. The two sample racks 30 can be replaced alternately as required, so that the influence on the detection of the samples caused by the replacement of the samples is avoided, and the detection efficiency is improved.
The sample holder 30 is provided with second lifting lugs 31 at both ends thereof. The sample rack 30 can be pushed, pulled, moved and the like through the second lifting lug 31, and convenience of operation is improved. In order to avoid interference and facilitate operation, the first lifting lug 24 is higher than the heights of the table tops at two ends of the first mounting groove 12.
The sample placing station 301 is provided with a first positioning portion 32, the sample bottle 40 is provided with a second positioning portion 45, and when the sample bottle 40 is placed in the sample placing station 301, the first positioning portion 32 corresponds to the second positioning portion 45. In this embodiment, the first positioning portion 32 is a positioning protrusion provided on the sample placement position 301, and the second positioning portion 45 is a positioning notch provided at the bottom of the sample bottle 40, and the positioning notch is deviated to one side of the sample bottle 40. If the sample bottle 40 is placed in the opposite direction or the sample bottle 40 is rotated by a certain angle, the sample bottle 40 cannot be placed in the sample bottle holder, and the first positioning portion 32 and the second positioning portion 45 are arranged, so that the direction of the sample bottle 40 can be kept consistent, and the pipette 96 can be correctly inserted into the sample extraction side 442 to complete the sample enrichment and transfer operation.
The structure of the sample bottle 40 used in this embodiment is mainly shown in fig. 10 to 13: the sample bottle 40 includes an inner bottle 42 and an outer bottle 41, the inner bottle 42 is disposed in the outer bottle 41, the inner bottle 42 is located on one side of the outer bottle 41, a first space 431 is formed in the inner bottle 42, a second space 432 is formed between the inner bottle 42 and the outer bottle 41, and the filter screen 421 is disposed at the bottom of the inner bottle 42 and located on the opposite side of the second space 432. The inner bottle 42 has a lower bottom near the second space 432 and a higher bottom far from the second space 432, and has an inclined second inclined bottom 423, the outer bottle 41 has a first inclined bottom 412 at the bottom, the first inclined bottom 412 and the second inclined bottom 423 have the same inclination direction, the second inclined bottom 423 is provided with a lower opening, and the strainer 421 is provided at the lower opening.
The inner bottle 42 and the outer bottle 41 are both provided with upper openings, the edge of the upper opening of the outer bottle 41 is provided with an external thread 413, the internal thread on the inner side of the bottle cap 46 is matched with the external thread 413 and covers the upper opening of the outer bottle 41, the radial inner side of the upper surface of the bottle cap 46 is partially recessed to form an internal groove 461, and when the suction pipe 96 is inserted, the tilting or slipping of the suction pipe can be effectively avoided. A side of the upper opening of the inner bottle 42 is a sample-putting side 441, and the sample-putting side 441 corresponds to the first space 431; the position opposite to the second space 432 between the inner bottle 42 and the outer bottle 41 is a sample taking side 442.
The sample bottle 40 of the present embodiment has a partition structure, and has a first space 431 and a second space 432, wherein a side of an upper opening of the sample bottle 40 opposite to the first space 431 forms a sample input side 441, and a side of the upper opening opposite to the second space 432 forms a sample extraction side 442; when the detection is carried out, a sample is placed from the sample placing side 441 of the sample bottle 40, the suction pipe 96 is inserted from the sample extracting side 442, the reagent in the sample bottle 40 is repeatedly sucked through the suction pipe 96, the target cells are separated from the sample, the suction pipe 96 is used for extracting, the sample is enriched and transferred, the sample can be conveniently transferred and enriched through the structure, the structure is relatively simple, and the operation is more convenient.
The filter screen 421 is located on the opposite side of the second space 432, and when the liquid is repeatedly sucked through the suction pipe 96, the structure can play a role in buffering and balancing, so that the phenomenon that the liquid has strong impact on the local part of the filter screen 421 by liquid flow under the action of the suction pipe 96 to influence the extraction of target cells of a sample is avoided, and the generation of impurities is also reduced. After the sample is repeatedly washed by the liquid flow, the target cells in the sample are enriched towards the bottom of the outer bottle 41 through the first inclined bottom 412, so that the consumption of the required sample is reduced, and the use cost of the sample and consumables is reduced.
The inner wall of the outer bottle 41 is provided with a first anti-reverse protrusion 411, the outer wall of the inner bottle 42 is provided with a second anti-reverse protrusion 422, the first anti-reverse protrusion 411 is close to one side of the second space 432, the inner bottle 42 is only put in according to a set direction, if the inner bottle 42 is inclined when put in, the outer bottle 41 cannot be put in the inner bottle 42, and the position of the inner bottle 42 in the outer bottle 41 can be ensured through the structure. The sample bottle 40 has a double-layer structure, and when in use, the outer bottle 41 can be used alone or the inner bottle 42 can be used in combination with the outer bottle 41 according to different detection purposes and different instruments used.
Please refer to fig. 1, fig. 6, fig. 8 and fig. 9, a plurality of barrier strips 81 are disposed on the base 10 to form a shielding member, and when the sample bottle 40 is placed at the sample placing position 301, the shielding member corresponds to the sample bottle 40; wherein, a plurality of blend stops 81 are parallel to each other, all form a hole 82 between a plurality of blend stops 81, when the suction head 93 moves up, can avoid the straw 96 to drive sample bottle 40 and move up, the width of a hole 82 is less than the width of sample bottle 40, can avoid sample bottle 40 to deviate from a hole 82, also can avoid the straw 96 to insert from sample side of putting in 441, causes the sample pollution.
A pipe removing piece 83 is arranged on the machine base 10, the pipe removing piece 83 is located at the side of the second mounting groove 13, and a pipe removing notch 831 is arranged on the pipe removing piece 83. The upper surface of the machine base 10 is provided with a recovery port 16, the recovery port 16 is located below the duct removing plate 83, the machine base 10 is provided with a third installation groove 14, a recovery box 15 is placed in the third installation groove 14 and is in sliding fit with the third installation groove 14, the recovery box 15 is located below the recovery port 16, a recovery cavity 152 is arranged in the recovery box 15, and a handle 151 is arranged on the outer side of the recovery box 15. After the suction pipes 96 are used, the mechanical arm 112 drives the suction heads 93 to move to the pipe-detaching notches 831, the suction heads 93 are lifted upwards, the suction pipes 96 fall off from the suction heads 93 and fall into the recycling bin 15, when the suction heads 93 in the recycling bin 15 reach a certain number, the recycling bin 15 can be drawn out of the third mounting groove 14, the suction pipes 96 in the recycling bin 15 are discarded and recycled, and after recycling, the recycling bin 15 slides into the third mounting groove 14.
The machine base 10 is further provided with a waste liquid tank 95 and a liquid bottle 94, and the machine base 11 is provided with a pump body (not shown in the figure) which is connected with the suction head 93 and the liquid bottle 94 through connecting pipes. The suction tube 96 at the end of the suction head 93 can be pressure-controlled by the pump body to perform a suction operation of a reagent or a sample, and the entire line can be cleaned, emptied, and the like by using absolute ethanol in the liquid bottle 94.
The operation method of the slice-making and dyeing machine in this embodiment is as follows:
1. preparation of
Electrifying and starting the dyeing machine;
the bottle cap 46 of the sample bottle 40 is opened, the inner bottle 42 is placed into the outer bottle 41, when the inner bottle 42 is placed, the matching direction between the first anti-reverse protrusion 411 and the inner bottle 42 is kept track of, and if the direction is staggered, the inner bottle 42 cannot be placed; the cell preservation solution is put into the sample bottle 40 in advance, and after the detection sample is put into the sample putting side 441 of the sample bottle 40, the bottle cap 46 is covered; after a plurality of samples are collected by the plurality of sample bottles 40, the plurality of sample bottles 40 are respectively placed in the sample placing positions 301 of the sample rack 30; the sample rack 30 with the samples is slid into the second mounting groove 13, when the sample slot slides in place, the magnetic block 302 is used for magnetic attraction locking, and if the sample rack 30 does not slide in place, the second position sensing element 303 is used for sensing and displaying or prompting outwards; after the sample rack 30 slides in place, the sample putting side 441 is shielded by the barrier strips 81 of the sample rack 30, and the sample extracting side 442 is located between the two barrier strips 81;
sequentially inserting a plurality of pipettes 96 into the pipette placing positions 22 on the pipe rack 20, and sliding the pipe rack 20 into which the pipettes 96 are inserted into the first mounting groove 12; if the pipe support 20 slides in place, the pipe support 20 is locked by the first locking part 21, the pipe support 20 cannot easily fall off, and if the pipe support 20 does not slide in place, the first position sensing element 23 can display or prompt outwards after sensing;
2. extraction pipette 96
3. emptying and cleaning
Pouring absolute alcohol into the liquid bottle 94, starting the pump body, transferring the absolute alcohol in the liquid bottle 94 to the suction head 93 through the hose for cleaning and emptying the pipeline and the suction head 93, and discharging the used absolute alcohol into the waste liquid tank 95 for temporary storage;
4. enrichment of
Under the action of the pump body, the suction head 93 sucks liquid repeatedly, liquid flows are formed on two sides of the filter screen 421, when the suction head 93 sucks the liquid, the liquid flows drive the sample cell solution on the sample putting side 441 to screen out target cells through the filter screen 421 and enter the sample extraction side 442, when the suction head 93 discharges the liquid, the liquid flows wash away sample impurities blocked on the filter screen 421, the sample cell solution on the sample putting side 441 is repeatedly filtered through repeated suction of the suction head 93, and finally, a sufficient number of target cells are enriched to the sample extraction side 442.
5. Transfer of
Under the action of the pump body, the suction head 93 sucks the reagent with the sample into the suction pipe 96, the mechanical arm 112 moves, and the sample is transferred and dripped onto the slide glass on the slide-making staining plate 72;
6. dyeing and flaking
The mechanical arm 112 moves, the suction head 93 moves downwards and extracts the dye solution from the dye solution rack 71, the dye solution is dripped onto the slide on the slide preparation dyeing plate 72, and the mixture is kept still;
7. the slide is taken out for observation and diagnosis.
The above embodiments are provided to illustrate, reproduce and deduce the technical solutions of the present invention, and to fully describe the technical solutions, the objects and the effects of the present invention, so as to make the public more thoroughly and comprehensively understand the disclosure of the present invention, and not to limit the protection scope of the present invention.
The above examples are not intended to be exhaustive of the invention and there may be many other embodiments not listed. Any alterations and modifications without departing from the spirit of the invention are within the scope of the invention.
Claims (15)
1. The sample bottle is characterized by comprising a bottle body, wherein the bottle body is provided with an upper opening, a filter screen is arranged in the bottle body, and the filter screen divides the bottle body into a first space and a second space; the side of the upper opening opposite to the first space is a sample placing side, and the side of the upper opening opposite to the second space is a sample extracting side.
2. The specimen bottle according to claim 1, wherein the bottle body comprises an inner bottle and an outer bottle, the inner bottle is disposed in the outer bottle and the inner bottle is disposed on one side of the outer bottle, the first space is formed in the inner bottle, the second space is formed between the inner bottle and the outer bottle, and the filter is disposed on the inner bottle.
3. The specimen bottle of claim 2, wherein the filter screen is disposed at the bottom of the inner bottle and on an opposite side of the second space.
4. The specimen bottle according to claim 2, wherein the bottom of the inner bottle is lower on the side close to the second space and higher on the side far from the second space to form a second inclined bottom in an inclined shape, the bottom of the outer bottle forms a first inclined bottom, the first inclined bottom and the second inclined bottom are inclined in the same direction, and the second inclined bottom is provided with a lower opening provided with the filter screen.
5. The specimen vial of claim 2, wherein a first anti-reverse bulge is provided on an inner wall of the outer vial.
6. The sample vial of claim 2, further comprising a cap that engages the upper opening, wherein a radially inner side of an upper surface of the cap is partially recessed to form an inner recess that is opposite at least the second space of the vial body.
7. The sample placing device is characterized by comprising a base and a sample frame, wherein a second mounting groove is formed in the base, the sample frame is placed in the second mounting groove and is in sliding fit with the second mounting groove, a plurality of sample placing positions for placing the sample bottles according to claim 1 are formed in the sample frame, the sample bottles are placed in the sample placing positions, a shielding piece is arranged on the base and is provided with a shielding part and a position yielding part, the shielding part corresponds to the sample bottles, and the position yielding part corresponds to the sample extracting side.
8. The specimen positioning apparatus of claim 7, wherein the shielding member is a plurality of shielding strips disposed on the base, the plurality of shielding strips are disposed in parallel, a plurality of openings are formed between the plurality of shielding strips, the shielding strips form the shielding portion, and the openings form the positioning portions.
9. The sample placement device according to claim 8, wherein the width of the strip aperture is less than the width of the sample vial.
10. The specimen placement device according to claim 7, wherein a second locking member is provided between said specimen rack and said second mounting slot.
11. A slide-making dyeing machine, characterized in that a frame, a pipe frame, a dye solution frame, a slide-making dyeing plate and the sample placing device of claim 7 are arranged on the machine base, a plurality of dye solution placing positions are arranged on the dye solution frame, and a plurality of slide placing positions are arranged on the slide-making dyeing plate; the frame is provided with a mechanical arm, and the mechanical arm is provided with a suction head facing to the pipe frame, the sample frame, the dyeing liquid frame and the slice-making dyeing plate.
12. The method for slice-making and dyeing is characterized by comprising the following steps:
placing the sample into a sample-placing side of the sample bottle;
repeatedly sucking liquid through the suction head, driving the liquid to form liquid flows on two sides of the filter screen, and screening target cells of the sample by the liquid flows to enter a sample extraction side;
repeatedly filtering to enrich the target cells of the sample to the sample extraction side;
sucking the liquid and the target cells on the sample extraction side through a suction pipe, and transferring to a slice preparation staining plate;
staining the target cells;
the stained specimens on the slide staining plate were examined.
13. The method for slice staining of claim 12,
in the previous step, when liquid is extracted through the suction head, the liquid flow drives the target cells on the sample putting side to enter the sample extracting side; when the liquid is discharged through the suction head, the liquid flow washes away the sample impurities blocked on the filter screen.
14. The method for sheet dyeing according to claim 12, wherein in the above step, before the test, the air in the suction head and the suction tube is evacuated by sucking the cleaning liquid in the liquid bottle by the pump, the excess liquid is stored in the waste liquid tank, and after the test, the liquid in the waste liquid tank is sucked into the suction head and the suction tube by the pump for cleaning the suction head and the suction tube.
15. The method for slice-making and dyeing according to claim 12, wherein in the preceding step, the robot arm moves to move the suction head downward and press the pipette on the rack into the suction head.
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| CN202110470346.8A CN113145191B (en) | 2021-04-28 | 2021-04-28 | Sample bottle, sample placement device, slide-making dyeing machine and slide-making dyeing method |
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| CN202110470346.8A CN113145191B (en) | 2021-04-28 | 2021-04-28 | Sample bottle, sample placement device, slide-making dyeing machine and slide-making dyeing method |
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| CN113145191B (en) | 2024-02-20 |
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