CN202471476U - One-step smear preparation device for liquid-based cytology smears - Google Patents
One-step smear preparation device for liquid-based cytology smears Download PDFInfo
- Publication number
- CN202471476U CN202471476U CN2012200064531U CN201220006453U CN202471476U CN 202471476 U CN202471476 U CN 202471476U CN 2012200064531 U CN2012200064531 U CN 2012200064531U CN 201220006453 U CN201220006453 U CN 201220006453U CN 202471476 U CN202471476 U CN 202471476U
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- bottle
- smear
- film
- making
- smear preparation
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Abstract
The utility model discloses a one-step smear preparation device for liquid-based cytology smears. The one-step smear preparation device for the liquid-based cytology smears comprises a sample centrifugal machine, a hanging basket disposed on the sample centrifugal machine and a smear preparation bottle reversely placed in the hanging basket. The smear preparation bottle comprises a bottle cover, a bottle body and a filter net disposed between the bottle cover and the bottle body. Slide loading holes for loading glass slides are symmetrically disposed on the left and right side walls of the bottle cover. The size of the slide loading holes is slightly larger than that of the glass slides. After the smear preparation bottle containing sample cells is oscillated, the glass slides are loaded in the smear preparation bottle, then the smear preparation bottle is centrifuged, and smear preparation is completed in one step. The one-step smear preparation device for the liquid-based cytology smears achieves the effect of combining a preservation liquid bottle and a centrifugation smear-preparation bin into one, simplifies procedures of centrifugation smear-preparation, eliminates a transferring step of a smear-preparation procedure, achieves the procedures of cell analysis, cell precipitation and the smear preparation in one step and improves stability of smear preparation quality. As a cell smear preparation device, the one-step smear preparation device for the liquid-based cytology smears is applied to the field of cell smear preparation.
Description
Technical field
The utility model relates to a kind of pelletizer, especially a kind of one step of liquid based cytology smear pelletizer.
Background technology
Medically, the doctor can be through the observation for cell smear, diseases such as diagnosis women cervical carcinoma.Traditionally, manual method is adopted in the cell film-making, and promptly the doctor will gather sample by hand and be coated in equably on the glass sheet, through alcohol fixation, dye, and examine under a microscope then.And along with the development of modern medicine, this employing traditional hand method is carried out the cell film-making and is progressively eliminated.
Now; The application of cell tabletting technology mainly contains two kinds: the one, and membrane type thin-layer cell technology (TCT technology); Be that the cell that collects is put into the specimen bottle that cell-preservation liquid is housed; Use stirrer dispersin polymerization cell particulate together, utilize suction function that cell is adsorbed on the filter membrane surface again, at last the cell transfer on the filtrator is processed a cell monolayer smear to microslide; The 2nd, liquid basal cell learn a skill (LCT technology); Be preservation liquid with collecting cell carry out proportion centrifugal after; Mucus in the sample, blood and inflammatory cell are separated; Collect remaining cell, process thin-layer cell on microslide, comprise discrete steps such as sample pre-treatment, sample transfer, separation and Extraction, film-making.But all there is certain defective in two kinds of flaking methods.Particularly, the shortcoming of TCT technology is to lose the part cell easily, and slice-making quality is unstable, and length consuming time, and consumptive material is expensive etc.The shortcoming of LCT technology then is the completion that need distribute of film-making step, and complex steps and equipment needed thereby are too much etc.
The utility model content
To the defective that existing cell tabletting technology exists, the purpose of the utility model provides a kind of one step of the liquid based cytology smear pelletizer that can simplify film-making flow process and raising slice-making quality stability.
One step of the utility model liquid based cytology smear pelletizer; Comprise the sample hydro-extractor, be installed in the Hanging Basket on the sample hydro-extractor and oppositely be placed into the film-making bottle in the Hanging Basket; The film-making bottle comprise bottle cap, bottle and be installed on bottle cap and bottle between filter screen; Bottle cap sidewall left-right symmetric has the load hole of loading microslide, and bore size is slightly larger than microslide.
Further, filter screen is a circular configuration, comprises screen pack and fixed mount; The filter screen size is coincide with bottle body and bottleneck.
Further, the filter screen fixed mount is provided with O-ring seal, is used for the film-making bottle is sealed, and makes bottle, bottle cap and filter screen to being installed in the even distribution of microslide pressure in the load hole.
Further, film-making bottle bottle inside bottom is provided with the radial line of inclination along medial surface.
Further, Hanging Basket is the rectangular slot structure, and inside dimension and microslide size are coincide; Level is installed on the sample hydro-extractor, and during film-making, film-making bottle cap symmetry is down put into Hanging Basket; Cover sample hydro-extractor loam cake, realize the centrifugal treating of sample hydro-extractor the film-making bottle.
Further, according to centrifugal needs, the rotating speed and the centrifugation time of sample hydro-extractor are regulated.
The beneficial effect of the utility model is: use one step of the utility model liquid based cytology smear pelletizer; Have the film-making bottle that loads microslide load hole through designing a kind of bottle cap sidewall left-right symmetric, after having loaded microslide, the film-making bottle oppositely put into be installed in that Hanging Basket carries out centrifugal treating on the sample hydro-extractor; Accomplish film-making; Thereby realized to preserve the effect that liquid bottle and centrifugal film-making storehouse unite two into one, not only simplified the film-making flow process, and made the sample cell that collects all participate in film-making; Guarantee complete sample information, improved the stability of the slice-making quality of knowing clearly.
Description of drawings
Be described further below in conjunction with the embodiment of accompanying drawing the utility model:
Fig. 1 is the wiring layout of one step of the utility model liquid based cytology smear pelletizer;
Fig. 2 is the structural drawing of film-making bottle cap in one step of the utility model liquid based cytology smear pelletizer;
Fig. 3 is the structural drawing of film-making bottle bottle in one step of the utility model liquid based cytology smear pelletizer;
Fig. 4 is the structural drawing of film-making bottle filter screen in one step of the utility model liquid based cytology smear pelletizer;
Fig. 5 is cradle structure figure in one step of the utility model liquid based cytology smear pelletizer.
Embodiment
With reference to Fig. 1, one step of the utility model liquid based cytology smear pelletizer comprises the sample hydro-extractor, is installed in the Hanging Basket on the sample hydro-extractor and oppositely is placed into the film-making bottle in the Hanging Basket.The sample hydro-extractor that Hanging Basket is installed among the figure does not show.Wherein, the film-making bottle comprise bottle cap 1, bottle 2 and be installed on bottle cap 1 and bottle 2 between filter screen 3.
With reference to Fig. 2; Film-making bottle cap 1 sidewall left-right symmetric has the load hole of loading microslide, and bore size is slightly larger than microslide, after the film-making bottle that the sample cell will be housed carries out centrifugal treating; With the microslide face down, level is inserted in the load hole 11 of film-making bottle cap 1 sidewall.
With reference to Fig. 3, film-making bottle bottle 2 is equipped with cell-preservation liquid and sampling brush brush when film-making, be used to preserve the sample cell that collection comes.The lateral surface of bottle 2 bottlenecks has screw thread, carries out screw thread with film-making bottle cap 1 and twists and to close.As the structure of further optimization, be provided with the radial line of inclination in film-making bottle bottle 2 inside bottom along medial surface, be used for helping to adhere to sample cell detachment on the sampling brush brush to cell-preservation liquid.As required, the angle of the shape of radial line, inclination and quantity can design.
With reference to Fig. 4, filter screen 3 is a circular configuration, comprises screen pack 32 and fixed mount 31, and filter screen 3 sizes are coincide with bottle 2 bottlenecks, are installed between film-making bottle cap 1 and the bottle 2.Filter screen 3 is used for filtering mucus and bulk impurity, only allows cast-off cells and less material from screen pack 32, to pass through.In addition, filter screen 3 can also stop the sampling that is placed in film-making bottle bottle 2 cell-preservation liquids to brush the direct impact of brush to microslide.Structure as further optimization; 31 are designed with O-ring seal on filter screen 3 fixed mounts; Not only can improve the sealing effectiveness of film-making bottle when making centrifugal treating; Prevent the seepage of cell-preservation liquid and sample cell, and help the even distribution that film-making bottle bottle 2, bottle cap 1 and 3 pairs of filter screens are loaded in microslide pressure in the bottle cap 1 load hole.
With reference to Fig. 5, Hanging Basket 4 is the rectangular slot structure, and inside dimension and microslide size are coincide; Level is installed on the sample hydro-extractor, and during film-making, film-making bottle cap 1 symmetry is down put into Hanging Basket 4; Cover sample hydro-extractor loam cake, realize the centrifugal treating of sample hydro-extractor the film-making bottle.
According to centrifugal needs, the rotating speed and the centrifugation time of sample hydro-extractor are regulated, realize that enrichment is controlled to the sample cell quantity on the microslide.
Describe adopting the utility model to carry out a step film-making with a more detailed embodiment below.
After completion that the sample cell is sampled, the sampling brush is put into the film-making bottle of filling cell-preservation liquid, light downwards pressure sampling brush brush holder support being taken off sampling brush brush and staying in the film-making bottle bottle 2.Place filter screen 3 at film-making bottle 2 bottlenecks, tighten bottle cap 1.
The film-making bottle is placed on the oscillator vibration 4-5 minute, and purpose is to make on the one hand the sample cell that is attached on the sampling brush brush to elute, and breaks up mucus on the other hand, and cell dispersion is suspended in the cell-preservation liquid all cells again equably.
Slowly turn on film-making bottle cap 1, the microslide face down is inserted in the load hole 11 of film-making bottle cap 1 leakproof of screwing.
With film-making bottle cap 1 down symmetry put into level and be installed in the Hanging Basket 4 on the sample hydro-extractor, cover sample hydro-extractor loam cake, configuring parameter of noncentricity: rotating speed 600-1000rpm behind the time 4-5min, begins centrifugal.
Sample hydro-extractor loam cake is opened in centrifugal end, takes out the film-making bottle together with microslide, and film-making bottle cap 1 is just put up, unscrews bottle cap 1, carefully slowly microslide is extracted out, accomplishes film-making.
In extracting the microslide process out, note not scraping and decrease the cell smear zone, guarantee the integrality of smear outward appearance.
After the film-making process finished, follow-up routine of carrying out was fixing and dye.
More than be that preferable enforcement to the utility model specifies; But the utility model is created and is not limited to said embodiment; Those of ordinary skill in the art make all equivalent variations or replacement under the prerequisite of the utility model spirit, also can doing, and distortion that these are equal to or replacement all are included in the application's claim institute restricted portion.
Claims (5)
1. liquid based cytology smear one goes on foot pelletizer; Comprise the sample hydro-extractor, be installed in the Hanging Basket on the sample hydro-extractor and oppositely be placed into the film-making bottle in the Hanging Basket; It is characterized in that: said film-making bottle comprise bottle cap, bottle and be installed on bottle cap and bottle between filter screen; Said bottle cap sidewall left-right symmetric has the load hole of loading microslide, and bore size is slightly larger than microslide.
2. one step of liquid based cytology smear pelletizer according to claim 1, it is characterized in that: said filter screen is a circular configuration, comprises screen pack and fixed mount; Said filter screen size is coincide with bottle body and bottleneck.
3. one step of liquid based cytology smear pelletizer according to claim 2; It is characterized in that: the filter screen fixed mount is provided with O-ring seal; Be used for the film-making bottle is sealed, make bottle, bottle cap and filter screen being installed in the even distribution of microslide pressure in the load hole.
4. one step of liquid based cytology smear pelletizer according to claim 1, it is characterized in that: said film-making bottle bottle inside bottom is provided with the radial line of inclination along medial surface.
5. one step of liquid based cytology smear pelletizer according to claim 1; It is characterized in that: said Hanging Basket is the rectangular slot structure, and inside dimension and microslide size are coincide, and level is installed on the sample hydro-extractor; During film-making; Film-making bottle cap symmetry is down put into Hanging Basket, covers sample hydro-extractor loam cake, realizes the centrifugal treating of sample hydro-extractor to the film-making bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200064531U CN202471476U (en) | 2012-01-09 | 2012-01-09 | One-step smear preparation device for liquid-based cytology smears |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200064531U CN202471476U (en) | 2012-01-09 | 2012-01-09 | One-step smear preparation device for liquid-based cytology smears |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202471476U true CN202471476U (en) | 2012-10-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012200064531U Expired - Lifetime CN202471476U (en) | 2012-01-09 | 2012-01-09 | One-step smear preparation device for liquid-based cytology smears |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202471476U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018030A (en) * | 2016-06-22 | 2016-10-12 | 杭州海世嘉生物科技有限公司 | Cell smearing device |
| CN106124266A (en) * | 2016-06-22 | 2016-11-16 | 杭州海世嘉生物科技有限公司 | A kind of liquid-based cell sample manufacturing method |
| WO2021042159A1 (en) * | 2019-09-02 | 2021-03-11 | Pang Tony | A device for immobilising rare cells for cytology |
-
2012
- 2012-01-09 CN CN2012200064531U patent/CN202471476U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018030A (en) * | 2016-06-22 | 2016-10-12 | 杭州海世嘉生物科技有限公司 | Cell smearing device |
| CN106124266A (en) * | 2016-06-22 | 2016-11-16 | 杭州海世嘉生物科技有限公司 | A kind of liquid-based cell sample manufacturing method |
| CN106018030B (en) * | 2016-06-22 | 2019-07-02 | 杭州海世嘉生物科技有限公司 | Cell smearing device |
| CN106124266B (en) * | 2016-06-22 | 2019-10-18 | 杭州海世嘉生物科技有限公司 | A kind of liquid-based cell sample manufacturing method |
| WO2021042159A1 (en) * | 2019-09-02 | 2021-03-11 | Pang Tony | A device for immobilising rare cells for cytology |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20121003 |