CN201032478Y - Liquid based thin layer cell processor - Google Patents
Liquid based thin layer cell processor Download PDFInfo
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- CN201032478Y CN201032478Y CNU2007200030553U CN200720003055U CN201032478Y CN 201032478 Y CN201032478 Y CN 201032478Y CN U2007200030553 U CNU2007200030553 U CN U2007200030553U CN 200720003055 U CN200720003055 U CN 200720003055U CN 201032478 Y CN201032478 Y CN 201032478Y
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Abstract
The utility model discloses a medical examination and biological study apparatus for smear pretreatment of cervical exfoliated cells, in particular to a liquid based thin cell layers processor to fully separate cell in samples from mucus and non-diagnosis component via eccentric plane-rotating oscillation. The utility model consists of a cell centrifugal bottle and a rotating oscillator. The cell centrifugal bottle comprises a bottle body structure with a double-layered conical bottle bottom, and the rotating oscillator comprises an eccentric plane-rotating oscillating platform structure. The cell centrifugal bottle contains cell treatment liquid; when being plane rotated and oscillated on the oscillation platform, the cell treatment liquid being mixed with the sample in the bottle will produce high speed laminar flow and horizontal centrifugation, so the cell and mucus and non-diagnosis component are fully separated, and the cells will conglomerate at the bottom of the double-layered conical bottle after stopping the rotating oscillator. The liquid based thin cell layers processor of the utility model can be conveniently used, and can rapidly, evenly and highly effectively pretreat the liquid based cells.
Description
Technical field
The utility model relates to and a kind of cervical exfoliated cell is carried out the medical test of smear pre-treatment and the instrument and equipment of biological study, is specially a kind of liquid based thin-layer cell processor that cell in the sample and mucus and non-diagnosis composition is fully separated by the flat rotating vibration of off-centre.
Background technology
By cervical exfoliated cell is carried out smear, dyeing, it is the important step of cytology check examination cervical carcinoma, general at present vertically centrifugal smear and the filtering membrane marking smear of concentrating of test tube that adopt, these two kinds of methods all mainly rely on treating fluid the decomposition of mucus are realized separating of cell and mucus and non-diagnosis composition, often separate fully inadequately, the shortcoming that has cell transition gathering or density inequality, has the cell of diagnostic value to be covered or be filtered.
Summary of the invention
The purpose of this utility model provide a kind of easy to use, can high efficiency separation cell and mucus and non-diagnosis composition in the sample, be convenient to the liquid based thin-layer cell processor of even smear.
For achieving the above object, the utility model liquid based thin-layer cell processor is made of jointly cell centrifugation bottle, gyrate shaker.The bottom of its cell centrifugation bottle has the Bottle structure at the bottom of the conical flask; Gyrate shaker has eccentric flat rotating vibration platform structure.
Above-mentioned liquid based thin-layer cell processor, the bottom of wherein said cell centrifugation bottle is a double-decker, at the bottom of wherein inner wall layer is conical flask.
Above-mentioned liquid based thin-layer cell processor, eccentric flat rotating vibration platform links by eccentric wheel and drive motor on its gyrate shaker, what vibration platform upper surface was provided with that several place the cell centrifugation bottles has a Di Yuan hole, and supporting with cell centrifugation bottle elasticity, the cell centrifugation bottle is placed on having in the inner chamber of Di Yuan hole of vibration platform.
Above-mentioned liquid based thin-layer cell processor, its cell centrifugation bottle is the double layered cone bottom structure, at the bottom of inner wall layer was conical flask, outer wall layer was for to have Di Yuan hole inner chamber shape adapting with described, and bottleneck in the top has the lid that matches with it.
The utility model liquid based thin-layer cell processor, the technical scheme that is adopted is: by the high-frequency plane of vibration platform rotational oscillation, make the cell treating fluid that is mixed with sample in the cell centrifugation bottle produce centrifugal along the high speed Laminar Flow and the level of bottle circumferencial direction, under high speed laminar flow and horizontal action of centrifugal force, cell is fully separated with mucus and non-diagnosis composition, slow down at gyrate shaker and gradually to stop and subsequently in the static process, cell is because of the more heavy conical lower portion that combines in the cell centrifugation bottle of proportion, and double layered cone internal layer pyramidal structure can make cell along cell centrifugation bottle tube wall smoothly to the conical lower portion sedimentation, layer structure makes that the garden hole shape of placing culture flask on shape and the vibration platform of cell centrifugation bottle is suitable, has increased the stability of bottle.
Said process is turned on the cell centrifugation bottle cap after finishing, and inhales and removes the above partially liq of bottle inner cone, and the cell mixture of conical lower portion enrichment can carry out follow-up smear, dying operation.
The utility model liquid based thin-layer cell processor and existing test tube be centrifugal smear and the filtering membrane marking smear of concentrating vertically, and the outstanding advantage of comparing is:
1, cell is abundant with separating of mucus and non-diagnosis composition, can significantly reduce because of the vertical centrifuge method of test tube insufficiently makes on the smear cell transition assemble even agglomerating influence to detection accuracy to what the sample cell separated with mucus, also can avoid filtration method there being the cell filtration of diagnostic value to fall the omission that is caused.
2, visible component all evenly is enriched at the bottom of the double layered cone bottle of cell centrifugation bottle in the tested sample, and reliable sample guarantee will be provided follow-up even smear, can improve the abnormal cell recall rate greatly after smear, dyeing.
The utility model liquid based thin-layer cell processor and the similar vertical centrifuge method comparison of test tube: 1, cost reduces significantly; the vertical centrifuge method film-making of similar test tube; need to be equipped with special oscillator and hydro-extractor; import equipment quotation (is example with someiyoshine board hydro-extractor) is more than 100,000 yuans; homemade hydro-extractor quotation (is example with Hunan, Changsha instrument hydro-extractor) is also ten thousand yuans of 2-3; the utility model liquid based thin-layer cell processor is the unifications of two machines; only 3000 yuans of quotations make things convenient for basic hospital to promote the popularization use.2, because the vibration of sample and centrifugally all carry out in the cell centrifugation bottle and once finish has reduced operation link, very simple and efficient.3, the circular hole quantity on the vibration platform can become different size for selecting for use according to the specimen amount size design, and maximum specification can be established 36 holes, and the single job time can finish in 5 minutes, thereby sample disposal efficient height.
The utility model is described in further detail below in conjunction with drawings and Examples.
Description of drawings
Figure 1 shows that the cell centrifugation bottle front view cross-sectional schematic in the utility model liquid based thin-layer cell processor.
Fig. 2 is the front view cross-sectional schematic in the vibration terraced garden hole inner chamber that cell centrifugation bottle shown in Figure 1 is installed in gyrate shaker.
Fig. 3 is a vibration platform vertical view synoptic diagram.
Among the figure 1, at the bottom of the double layered cone bottle; 2, the cell centrifugation bottle; 3, the cell centrifugation bottle cap; 4, drive motor; 5, eccentric wheel; 6, the vibration platform; 7, the Di Yuan hole is arranged.
Embodiment
As shown in Figure 1, the cell centrifugation bottle of the utility model liquid based thin-layer cell processor comprises having at the bottom of the double layered cone bottle 1 Bottle structure, and its inner wall layer is taper, and outer wall layer is a cylindrical shape, covers 3 in that the bottleneck of the end of bottle 2 is supporting.
As shown in Figure 2, the gyrate shaker of the utility model liquid based thin-layer cell processor includes vibration platform 6, links by eccentric wheel 5 and drive motor 4, and the torque of drive motor 4 sends vibration platform 6 to by eccentric wheel 5, makes it produce the plane and rotatablely moves.
Cell centrifugation bottle bottle 2 is placed on having in 7 inner chambers of Di Yuan hole of vibration platform 6.
Embodiment: the utility model liquid based thin-layer cell processor is applied to gynaecology's cervical carcinoma screening for example.
Have now in the flaking method based on vertical centrifuge method of test tube and filtration method film-making, these two kinds of flaking methods are all abundant inadequately with separating of mucus and non-diagnosis composition because of cell in the sample, easily cause the cell transition to assemble, have the cell of diagnostic value to be covered or be filtered, cause the possibility of omission bigger, cervical carcinoma examination omission then may bring fatal influence to the patient, because cervical carcinoma is the cancer that present unique that the World Health Organization (WHO) generally acknowledges can early prevent, early check, early cure.
Concrete operation method is as follows:
1, cell centrifugation bottle cap 3 is opened the cell treating fluid that the 15ml that packs into contains 80% alcohol, the sampling brushhead that will have cervical exfoliated cell is put into bottle and the brush holder that fractures, and screws bottle cap 3.
That 2, will pack that the cell centrifugation bottle of sample places gyrate shaker vibration platform 6 into has Di Yuan hole 7 inner chambers.With 1800 rev/mins of rotational oscillations 5 minutes.
3, on vibration platform 6, take out the cell centrifugation bottle, open bottle cap 3, remove the sampling brush, remove cell centrifugation bottle straight-tube portion liquid with the sample-sucking device suction after static 3 minutes.
4, with sample injector 1 taking-up 1ml cell mixture at the bottom of cell centrifugation bottle double layered cone bottle, be added in the film-making box of centrifugal smearing machine, finish smear, dyeing and drying, mounting.
By above operation can obtain that smear is even, cell is complete, the high-quality cytology Pathologic specimen smear of missed diagnosis composition not.
Liquid based thin-layer cell processor not only is applied to cell smear, also can be used for the check and the research field of projects such as bacterium, sperm, parasite.
Claims (4)
1. a liquid based thin-layer cell processor comprises a cell centrifugation bottle and a gyrate shaker, it is characterized in that: described cell centrifugation bottle bottom has the Bottle structure at the bottom of the conical flask, and described gyrate shaker includes eccentric flat rotating vibration platform structure.
2. liquid based thin-layer cell processor according to claim 1 is characterized in that: the bottom of described cell centrifugation bottle is a double-decker, at the bottom of wherein inner wall layer is conical flask.
3. liquid based thin-layer cell processor according to claim 1 and 2, it is characterized in that: eccentric flat rotating vibration platform links by eccentric wheel and drive motor on the described gyrate shaker, what vibration platform upper surface was provided with a plurality of placement cell centrifugation bottles has a Di Yuan hole, and supporting with described cell centrifugation bottle elasticity, the cell centrifugation bottle is placed on having in the inner chamber of Di Yuan hole of vibration platform.
4. according to claim 1 or 3 described liquid based thin-layer cell processors, it is characterized in that: the cell centrifugation bottle is the double layered cone bottom structure, at the bottom of inner wall layer was conical flask, outer wall layer was for to have Di Yuan hole inner chamber shape adapting with described, and bottleneck in the top has the lid that matches with it.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200030553U CN201032478Y (en) | 2007-02-28 | 2007-02-28 | Liquid based thin layer cell processor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200030553U CN201032478Y (en) | 2007-02-28 | 2007-02-28 | Liquid based thin layer cell processor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201032478Y true CN201032478Y (en) | 2008-03-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200030553U Expired - Fee Related CN201032478Y (en) | 2007-02-28 | 2007-02-28 | Liquid based thin layer cell processor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539211A (en) * | 2012-01-09 | 2012-07-04 | 广州三瑞医疗器械有限公司 | Device and method for making liquid-based cytological smear in one step |
| CN106124266A (en) * | 2016-06-22 | 2016-11-16 | 杭州海世嘉生物科技有限公司 | A kind of liquid-based cell sample manufacturing method |
| CN107063804A (en) * | 2017-05-08 | 2017-08-18 | 深圳市孔雀生物科技有限公司 | A kind of accurate smear methods of airflow classification competent cell |
-
2007
- 2007-02-28 CN CNU2007200030553U patent/CN201032478Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539211A (en) * | 2012-01-09 | 2012-07-04 | 广州三瑞医疗器械有限公司 | Device and method for making liquid-based cytological smear in one step |
| CN106124266A (en) * | 2016-06-22 | 2016-11-16 | 杭州海世嘉生物科技有限公司 | A kind of liquid-based cell sample manufacturing method |
| CN106124266B (en) * | 2016-06-22 | 2019-10-18 | 杭州海世嘉生物科技有限公司 | A kind of liquid-based cell sample manufacturing method |
| CN107063804A (en) * | 2017-05-08 | 2017-08-18 | 深圳市孔雀生物科技有限公司 | A kind of accurate smear methods of airflow classification competent cell |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080305 Termination date: 20120228 |