CN115477993A - AI intelligent adipose-derived stem cell culture method - Google Patents
AI intelligent adipose-derived stem cell culture method Download PDFInfo
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 34
- 238000004113 cell culture Methods 0.000 title claims abstract description 19
- 238000003756 stirring Methods 0.000 claims abstract description 64
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 230000000903 blocking effect Effects 0.000 claims abstract description 25
- 238000000926 separation method Methods 0.000 claims abstract description 18
- 238000011049 filling Methods 0.000 claims abstract description 14
- 210000000577 adipose tissue Anatomy 0.000 claims abstract description 13
- 210000001519 tissue Anatomy 0.000 claims abstract description 13
- 238000000605 extraction Methods 0.000 claims abstract description 12
- 210000001593 brown adipocyte Anatomy 0.000 claims abstract description 8
- 210000004369 blood Anatomy 0.000 claims abstract description 7
- 239000008280 blood Substances 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000003925 fat Substances 0.000 claims description 38
- 210000004027 cell Anatomy 0.000 claims description 13
- 238000005096 rolling process Methods 0.000 claims description 12
- 230000029087 digestion Effects 0.000 claims description 9
- 239000006285 cell suspension Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 claims description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 210000001789 adipocyte Anatomy 0.000 claims description 3
- 210000000601 blood cell Anatomy 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000012894 fetal calf serum Substances 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 230000008929 regeneration Effects 0.000 claims description 3
- 238000011069 regeneration method Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- 230000004888 barrier function Effects 0.000 claims 2
- 238000004062 sedimentation Methods 0.000 abstract description 2
- 238000004904 shortening Methods 0.000 abstract description 2
- 238000002156 mixing Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000000746 purification Methods 0.000 description 2
- 235000013547 stew Nutrition 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005429 filling process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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Abstract
The invention provides an AI intelligent adipose-derived stem cell culture method, which comprises the following steps: s1, selecting fat of a targeted part, and extracting different raw materials before application according to different fat components contained in different parts, such as large, medium and small fat cells, brown fat cells or brown fat cells, and according to the amount and weakness of tissues in various regions of a body and different factors of blood; s2, extracting required fat components, and purifying and selecting fat with various densities; s3, filling the parts according to the concrete filling positions; compared with the comparison file, the separation and extraction equipment provided by the invention can be used for blocking the mixed liquid of the adipose tissues which generates the vortex due to stirring after the stirring is stopped on the basis of keeping the stirring work, damaging the vortex generated by the mixed liquid and rapidly standing the mixed liquid, thereby shortening the time for sedimentation and layering, greatly reducing the probability of the self-apoptosis of the adipose tissues and being convenient to use.
Description
Technical Field
The invention relates to the technical field of adipose-derived stem cell culture setting, in particular to an AI intelligent adipose-derived stem cell culture method.
Background
In the fat filling process, firstly, fat tissues are extracted from the body of a reshaper, fat stem cells are cultured after a series of treatments and then are filled and injected, the fat stem cells are injected immediately after the fat extraction and cultivation, most of the fat stem cells are simultaneously subjected to fat extraction and fat transplantation, the cultivation time is very short, if the cultivation time is too long, the fat is absorbed by the body or undergoes self-apoptosis, the transplantation effect is greatly reduced, and the prospect is possibly not reached;
according to the prior patent CN113621474A, an apparatus for extracting adipose-derived stem cells by negative pressure separation is disclosed, which comprises: the separation extractor is provided with a stirring device towards the inside of the upper cover, the upper cover is also provided with a to-be-treated mixed liquid inlet, the bottom of the separation extractor extends downwards to be provided with a guide pipe, the guide pipe is communicated with the inside of the separation extractor, and one end of the guide pipe is provided with a flow speed regulator; the collector comprises an upper-layer filtering part and a lower-layer collecting part which are movably connected, the other end of the conduit is inserted into the upper-layer filtering part, the bottom of the upper-layer filtering part is provided with a filter screen, and the upper part of the lower-layer collecting part is open; and the negative pressure device comprises a first pipeline, a negative pressure storage tank, a second pipeline and a suction device, the first pipeline enables the separation extractor to be communicated with the negative pressure storage tank, and the second pipeline enables the negative pressure storage tank to be communicated with the suction device. The device provided by the invention has the advantages of simple structure, convenience in operation and good sealing effect, and the filter screen can filter out residual impurities; the separation that this patent provided draws fat stem cell's equipment can carry out effectual stirring to the mixture of adipose tissue and treatment fluid through setting up rabbling mechanism, thereby can guarantee that the separation purification work high efficiency of fat goes on, but the in-process that stirs the mixture can make the internal mixture of jar produce the vortex, the vortex still can last longer time under inertial effect after stopping the stirring, therefore can not accomplish the work of deposiing with faster time, make the time of deposiing the layering cause the extension, and we know the work that the fat of taking out was cultivated and need accomplish in faster time, and this kind of equipment is then very big improvement the probability that the fat takes place to die by oneself, be not convenient for use.
Therefore, there is a need to provide a new AI intelligent adipose stem cell culture method to solve the above technical problems.
Disclosure of Invention
In order to solve the technical problems, the invention provides an AI intelligent adipose-derived stem cell culture method.
The invention provides an AI intelligent adipose-derived stem cell culture method, which comprises the following steps:
s1, selecting fat of a targeted part, and extracting different raw materials before application according to different fat components contained in different parts, such as large, medium and small fat cells, brown fat cells or brown fat cells, and according to the amount and weakness of tissues in various regions of a body and different factors of blood;
s2, extracting required fat components, and purifying and selecting fats with various densities;
s3, preparing SVF-gel and PRF with various densities according to specific filling positions for filling;
1) Extracting the sucked adipose tissues by a separation and extraction device, removing residual blood cells and tissue fragments, and then weighing and digesting;
2) Removing upper undigested adipose tissues after digestion, then removing supernatant after centrifuging the lower cell suspension for ten minutes at 1500r \ min by a centrifuge to obtain adipose-derived stem cell mass at the bottom of a centrifuge tube, then re-suspending the adipose-derived stem cell mass, adjusting the density to 1 × 105ml < -1 >, and then inoculating the adipose-derived stem cell mass in a cell culture flask;
3) After the stem cells grow over the culture bottle, passage is carried out according to one-to-two, the cells are washed once by using D-Hanks, then 2ml of mixed enzyme solution is added for digestion, after the cells are observed to be obviously deformed and shrunk into spheres under a microscope, the digestion is stopped by using a culture medium containing 10% fetal calf serum immediately, the bottom of the bottle is lightly blown by a pipette, the cells are ensured to be completely separated from the suspension, and then the cell suspension is centrifuged for 5min by a centrifuge at 1000r \min;
4) Finally, reuniting the cell clusters obtained by centrifugation, adjusting the density to be proper density and then inoculating;
s4, an AI fat filling technology is applied in a targeted manner, so that AI fat is managed at a filling part automatically, blood is continuously nourished, and each AI adipocyte can play a regeneration operation function automatically;
the separation and extraction equipment used in the step S3 comprises a tank body, a feed inlet is fixed at the top of the tank body, a first liquid outlet is fixed at the bottom of the tank body, a second liquid outlet is fixed on the side wall of the tank body, the feed inlet, the first liquid outlet and the second liquid outlet are communicated with the tank body, a stirring device used for stirring the fat tissue inside the tank body is connected to the middle of the tank body through a bearing in a rotating mode, a blocking device used for destroying the mixed liquid eddy current of the fat tissue formed due to stirring is connected to the side wall of the tank body through a bearing in a rotating mode, and the blocking device is linked with the stirring device.
Preferably, agitating unit includes (mixing) shaft, stirring leaf, motor, worm and worm wheel, and the middle part of the jar body is rotated through the bearing and is connected with the (mixing) shaft, and the outer wall symmetry equidistance of (mixing) shaft is fixed with the stirring leaf, and the stirring leaf is located the inboard of the jar body, and the top of the jar body is fixed with the motor, and the output of motor is fixed with the worm, and the worm passes through the bearing to be connected with the top of the jar body is rotated, and the top of (mixing) shaft is fixed with the worm wheel, and the worm wheel is connected with the worm meshing.
Preferably, the stopping device comprises an overturning rod, a torsion spring, a stopping plate and a linkage assembly, the overturning rod is symmetrically and rotatably connected to the side wall of the tank body through a bearing, the torsion spring is fixed to both ends of the overturning rod, one end, far away from the overturning rod, of the torsion spring is fixed to the inner wall of the tank body, the stopping plate is fixed to the middle of the overturning rod, the stopping plate is located on the inner side of the tank body, the linkage assembly is fixed to the top of the overturning rod, and one end of the linkage assembly is fixed to the stirring device.
Preferably, the two blocking plates are arranged on the inner side of the tank body in a central symmetry manner.
Preferably, the blocking plate is arranged in an arc shape.
Preferably, the linkage assembly comprises a transverse connection piece, a follow-up column, sliding sleeves, a transverse movement driving rod, a driving frame, a driving disc and universal rollers, wherein the transverse connection piece is fixed at the top of the turnover rod, the top of the transverse connection piece is rotatably connected with the follow-up column through a bearing, the two sliding sleeves are fixed at the top of the tank body, the inner wall of each sliding sleeve is slidably connected with the transverse movement driving rod, the driving frame is fixed at one end, close to the follow-up column, of the transverse movement driving rod, the follow-up column is in rolling connection with the inner wall of the driving frame, the driving disc is fixed at the end part of the worm, the universal rollers are fixed at one end, close to the driving disc, of the transverse movement driving rod, and the universal rollers are matched with the side walls of the driving disc.
Preferably, the side wall of the driving disc is symmetrically formed with a recess, and the universal roller is in pressing contact with the inner wall of the recess.
Preferably, the connecting portion between the two ends of the recess and the side wall of the driving disk is smoothly arranged.
Preferably, a hopper is fixed at the top of the feeding hole and is arranged in a shape of a big end up.
Preferably, the lower extreme equidistance of jar body outer wall is fixed with the bracing piece, and the bottom of bracing piece is fixed with the support base.
Compared with the related technology, the AI intelligent adipose-derived stem cell culture method provided by the invention has the following beneficial effects:
1. compared with the comparison document, the separation and extraction equipment provided by the invention has the advantages that the blocking device is arranged, on the basis of keeping the stirring work, the fat tissue mixed liquid which generates vortex due to stirring can be blocked after the stirring is stopped, the vortex generated by the fat tissue mixed liquid is damaged, and the fat tissue mixed liquid can be rapidly static, so that the time for sedimentation and layering is shortened, the probability of self-apoptosis of fat is greatly reduced, and the use is convenient;
2. the blocking device provided by the invention is in mechanical linkage with the stirring device, so that the timeliness of the blocking work is ensured, namely the blocking work can be started immediately after the stirring work is finished.
Drawings
FIG. 1 is a schematic view of the overall structure of the separation and extraction apparatus of the present invention;
FIG. 2 is a schematic view of a first port location configuration of the present invention;
FIG. 3 is a schematic view of a stirring device according to the present invention;
FIG. 4 is a second schematic structural view of a stirring device according to the present invention;
FIG. 5 is a schematic view of a blocking device according to the present invention;
FIG. 6 is a second schematic view of the blocking device of the present invention;
FIG. 7 is a schematic view of a linkage assembly of the present invention.
Reference numbers in the figures: 1. a tank body; 2. a feed inlet; 2a, a hopper; 3. a first liquid outlet; 4. a second liquid outlet; 5. a stirring device; 51. a stirring shaft; 52. stirring blades; 53. a motor; 54. a worm; 55. a worm; 6. a blocking device; 61. a turning rod; 62. a torsion spring; 63. a blocking plate; 64. a linkage assembly; 641. a transverse connecting sheet; 642. a follower post; 643. a sliding sleeve; 644. transversely moving the driving rod; 645. a drive frame; 646. a drive plate; 646a, a recess; 647. a universal roller; 7. a support bar; 8. a support base.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
Specific implementations of the present invention are described in detail below with reference to specific embodiments.
Referring to fig. 1 and 2, a method for culturing AI intelligent adipose-derived stem cells according to an embodiment of the present invention includes the following steps:
s1, selecting fat of a targeted part, and extracting different raw materials before application according to different fat components contained in different parts, such as large, medium and small fat cells, brown fat cells or brown fat cells, and according to the quantity and weakness of tissues in various regions of a body and different factors of blood;
s2, extracting required fat components, and purifying and selecting fats with various densities;
s3, preparing SVF-gel and PRF with various densities according to specific filling positions for filling;
1) Extracting the sucked adipose tissues by a separation and extraction device, removing residual blood cells and tissue fragments, and then weighing and digesting;
2) Removing undigested adipose tissues at the upper layer after digestion, removing supernatant after centrifuging the cell suspension at 1500r \ min375g for ten minutes by a centrifugal machine to obtain adipose-derived stem cell clusters at the bottom of a centrifugal tube, then re-suspending the adipose-derived stem cell clusters, adjusting the density to 1 × 105ml < -1 >, and then inoculating the adipose-derived stem cell clusters in a cell culture bottle;
3) After the stem cells are overgrown in a culture flask, passage is carried out by one-to-two, firstly, the cells are washed once by using D-Hanks, then, 2ml of mixed enzyme solution 0.25 percent EDTA is added for digestion, after the cells are observed to be obviously deformed and shrunk into spheres under a microscope, the digestion is immediately stopped by using a culture medium containing 10 percent fetal calf serum, the bottom of the flask is lightly blown by a pipette to ensure that the cells are completely separated from the suspension, and then, the cell suspension is centrifuged for 5min by 1000 \min167g through a centrifuge;
4) Finally, the cell mass obtained by centrifugation is gathered again, and inoculation is carried out after the density is adjusted to be proper;
s4, an AI fat filling technology is applied in a targeted manner, so that AI fat is managed at a filling part automatically, blood is continuously nourished, and each AI adipocyte can play a regeneration operation function automatically;
wherein separation extraction equipment that uses in step S3 includes a jar body 1, the top of jar body 1 is fixed with feed inlet 2, the bottom of jar body 1 is fixed with first liquid outlet 3, the lateral wall of jar body 1 is fixed with second liquid outlet 4, and feed inlet 2, first liquid outlet 3 and second liquid outlet 4 all are linked together with jar body 1, the middle part of jar body 1 is connected with through the bearing rotation and is used for carrying out the agitating unit 5 that stirs to the adipose tissue that inside was handled, it is connected with the blocking device 6 that is used for destroying the mixed liquid vortex of adipose tissue that the stirring leads to forming to rotate through the bearing on the lateral wall of jar body 1, and blocking device 6 and agitating unit 5 carry out the linkage.
It should be noted that, the separation extraction equipment that this application provided can not effectual shortening mixed liquid after the stirring layering time that stews in comparison with the reference file to make mixed liquid after the stirring can accomplish the layering work that stews comparatively fast, and then shortened the time of whole separation purification, thereby the effectual probability that reduces the fat emergence and die by oneself, more convenient to use.
Referring to fig. 3 and 4, the stirring device 5 includes a stirring shaft 51, stirring blades 52, a motor 53, a worm 54 and a worm gear 55, the middle of the tank body 1 is rotatably connected with the stirring shaft 51 through a bearing, the stirring blades 52 are symmetrically and equidistantly fixed on the outer wall of the stirring shaft 51, the stirring blades 52 are located on the inner side of the tank body 1, the motor 53 is fixed on the top of the tank body 1, the worm 54 is fixed on the output end of the motor 53, the worm 54 is rotatably connected with the top of the tank body 1 through a bearing, the worm gear 55 is fixed on the top of the stirring shaft 51, and the worm gear 55 is meshed with the worm 54.
During the use, rotate through driving motor 53, thereby drive worm 54 and rotate, and then stir worm wheel 55 and rotate, thereby the die stirring shaft 51 rotates, and then stir jar inboard mixed liquid of body 1 through stirring leaf 52, accomplish stirring work, and need emphasize, stirring work motor 53 all rotates fixed number of turns at every turn, and drive stirring shaft 51 only rotates corresponding fixed number of turns, promptly after stirring work at every turn, driving-disc 646 and stirring leaf 52 position homoenergetic reset.
Referring to fig. 5, 6 and 7, the stopping device 6 includes a turning rod 61, a torsion spring 62, a stopping plate 63 and a linkage assembly 64, the side wall of the tank 1 is symmetrically and rotatably connected with the turning rod 61 through a bearing, the torsion spring 62 is fixed at both ends of the turning rod 61, one end of the torsion spring 62 far away from the turning rod 61 is fixed with the inner wall of the tank 1, the stopping plate 63 is fixed at the middle part of the turning rod 61, the two stopping plates 63 are arranged in central symmetry with the inner side of the tank 1, the stopping plate 63 is arranged in arc shape, the stopping plate 63 is located at the inner side of the tank 1, the linkage assembly 64 is fixed at the top of the turning rod 61, one end of the linkage assembly 64 is fixed with the stirring device 5, the linkage assembly 64 includes a transverse connecting plate 641, a transverse moving column 642, a sliding sleeve 643, a transverse moving driving rod 644, a driving frame 645, a driving disk 647 and a universal roller 647, the top of the turning rod 61 is fixed with a transverse connecting column 642 through a bearing, the top of the tank 1 is fixed with two sliding sleeves 643, the inner wall of the sliding sleeve 643 is connected with a sliding sleeve 646, one end of the transverse moving column 647 and a roller 646 close to the driving rod 646, the driving disk 646 is connected with a rolling depression 646, the driving disk 646a rolling depression 646 of the driving rod 646 and a roller 646 fixed with the driving rod 646, the driving rod 646 and the rolling roller 646, the rolling roller 646a rolling depression 646 are connected with the driving rod 646, the driving disk 646, the rolling depression 646a of the rolling roller 646, the rolling roller 646 and the rolling roller 646.
When stirring work is carried out, because the motor 53 rotates fast, the worm 54 is driven to rotate fast, thereby the driving disc 646 is driven to rotate fast, and when the driving disc 646 rotates, the universal idler wheel 647 is pushed, the transverse rolling part rolls to the outer side wall of the driving disc 646 from the concave part 646a excessively, then the transverse driving rod is pushed to slide, the two transverse moving rods slide to one side far away from the driving disc 646 simultaneously, thereby the driving frame 645 is driven to move, the two overturning rods 61 can be driven to overturn simultaneously through the matching of the driving frame 645 and the driving column, thereby the blocking plate 63 is driven to overturn to the inner side wall of the tank body 1, thereby the blocking plate 63 forms avoidance to the stirring blades 52, and because the motor 53 rotates fast, the driving disc 646 rotates fast, thereby the universal idler wheel 647 can be inserted into the inner side of the concave part 646a for a short time, thereby when stirring work is carried out, the two blocking plates 63 can be stably positioned at avoidance positions, and at the stirring work is finished, the stirring shaft 51, the stirring blades 52 and the driving disc 646 stop rotating and reset, at this moment, the universal idler wheel 647 aligns to the torsion spring 62 a, then the inner side of the turning rod 61 is driven, thereby the inner side of the blocking plate can be reset to restore the vortex of the mixed liquid, thereby the mixed liquid can be restored in the state of the rotating pipe 647, and the pipe can be conveniently kept.
Referring to fig. 7, the connection portion between the two ends of the recess 646a and the side wall of the driving disc 646 is smoothly arranged, so that the gimbal roller 647 can smoothly roll onto the outer side wall of the driving disc 646.
Referring to fig. 1, a hopper 2a is fixed at the top of the feed inlet 2, and the hopper 2a is arranged in a shape of big end up and small end down, so that the mixed liquid can be conveniently added into the tank 1.
Referring to fig. 1, support rods 7 are fixed to the lower end of the outer wall of the tank 1 at equal intervals, and a support base 8 is fixed to the bottom of each support rod 7 and used for supporting the tank 1.
The circuits and controls involved in the present invention are prior art and will not be described in detail herein.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. An AI intelligent adipose-derived stem cell culture method is characterized by comprising the following steps:
s1, selecting fat of a targeted part, and extracting different raw materials before application according to different fat components contained in different parts, such as large, medium and small fat cells, brown fat cells or brown fat cells, and according to the amount and weakness of tissues in various regions of a body and different factors of blood;
s2, extracting required fat components, and purifying and selecting fats with various densities;
s3, preparing SVF-gel and PRF with various densities according to specific filling positions for filling;
1) Extracting the sucked adipose tissues by a separation and extraction device, removing residual blood cells and tissue fragments, and then weighing and digesting;
2) Removing upper undigested adipose tissues after digestion, removing supernatant after centrifuging the lower cell suspension for ten minutes at 1500r \min (375 g) by a centrifuge to obtain adipose stem cell mass at the bottom of the centrifuge tube, then re-suspending the cell mass, adjusting the density to 1 × 105ml < -1 >, and then inoculating the cell culture flask;
3) After the stem cells grow over the culture flask, passage is carried out by one-to-two passage, the cells are washed once by using D-Hanks, then 2ml of mixed enzyme solution (0.25 percent EDTA) is added for digestion, after the cells are obviously deformed and shrunk into a spherical shape under the microscope, the digestion is immediately stopped by using a culture medium containing 10 percent fetal calf serum, the bottom of the flask is lightly blown by using a pipette to ensure that the cells are completely separated from the suspension, and then the cell suspension is centrifuged for 5min at 1000r \min (167 g) by a centrifuge;
4) Finally, the cell mass obtained by centrifugation is gathered again, and inoculation is carried out after the density is adjusted to be proper;
s4, an AI fat filling technology is applied in a targeted manner, so that AI fat is automatically managed at a filling part, blood is continuously nourished, and each AI fat cell can automatically play a regeneration operation function;
wherein separation extraction equipment that uses in step S3 includes a jar body (1), the top of the jar body (1) is fixed with feed inlet (2), the bottom of the jar body (1) is fixed with first liquid outlet (3), the lateral wall of the jar body (1) is fixed with second liquid outlet (4), and feed inlet (2), first liquid outlet (3) and second liquid outlet (4) all are linked together with jar body (1), the middle part of the jar body (1) is connected with through the bearing rotation and is used for carrying out agitating unit (5) that stir to the adipose tissue that the inside was handled, be connected with through the bearing rotation on the lateral wall of the jar body (1) and be used for destroying barrier device (6) that the stirring leads to the adipose tissue mixed liquid vortex that forms, and barrier device (6) and agitating unit (5) carry out the linkage.
2. The AI intelligent adipose-derived stem cell culturing method according to claim 1, wherein the stirring device (5) comprises a stirring shaft (51), stirring blades (52), a motor (53), a worm (54) and a worm gear (55), the middle part of the tank body (1) is rotatably connected with the stirring shaft (51) through a bearing, the stirring blades (52) are symmetrically and equidistantly fixed on the outer wall of the stirring shaft (51), the stirring blades (52) are positioned on the inner side of the tank body (1), the motor (53) is fixed on the top of the tank body (1), the worm (54) is fixed on the output end of the motor (53), the worm (54) is rotatably connected with the top of the tank body (1) through a bearing, the worm gear (55) is fixed on the top of the stirring shaft (51), and the worm gear (55) is meshed with the worm (54).
3. The AI intelligent adipose-derived stem cell culture method according to claim 2, wherein the blocking device (6) comprises a turning rod (61), a torsion spring (62), a blocking plate (63) and a linkage assembly (64), the side wall of the tank body (1) is symmetrically and rotatably connected with the turning rod (61) through a bearing, the torsion spring (62) is fixed at both ends of the turning rod (61), one end of the torsion spring (62) far away from the turning rod (61) is fixed with the inner wall of the tank body (1), the blocking plate (63) is fixed in the middle of the turning rod (61), the blocking plate (63) is located at the inner side of the tank body (1), the linkage assembly (64) is fixed at the top of the turning rod (61), and one end of the linkage assembly (64) is fixed with the stirring device (5).
4. The AI intelligent adipose stem cell culture method of claim 3, wherein the two blocking plates (63) are arranged in a central symmetry with the inside of the tank (1).
5. The AI intelligent adipose stem cell culture method of claim 3, wherein the blocking plate (63) is arc-shaped.
6. The AI intelligent adipose-stem cell culture method of claim 5, wherein the linkage assembly (64) comprises a transverse connection sheet (641), a follow-up column (642), a sliding sleeve (643), a transverse movement driving rod (644), a driving frame (645), a driving disc (646) and universal rollers (647), the transverse connection sheet (641) is fixed on the top of the turning rod (61), the follow-up column (642) is rotatably connected on the top of the transverse connection sheet (641) through a bearing, the two sliding sleeves (643) are fixed on the top of the tank body (1), the transverse movement driving rod (644) is slidably connected on the inner wall of the sliding sleeve (643), the driving frame (644) is fixed on one end of the transverse movement driving rod (644) close to the follow-up column (642), the follow-up column (642) is in rolling connection with the inner wall of the driving frame (645), the driving disc (646) is fixed on the end of the worm (54), the universal rollers (647) are fixed on one end of the transverse movement driving rod (644) close to the driving disc (646), and the universal rollers (647) are matched with the side walls of the driving disc (646).
7. The AI intelligent adipose stem cell culture method of claim 6, wherein the driving disc (646) has side walls symmetrically formed with recesses (646 a), and the gimbaled rollers (647) are in pressing contact with the inner walls of the recesses (646 a).
8. The AI intelligent adipose stem cell culture method of claim 7, wherein the two ends of the recess part (646 a) are smoothly and excessively connected with the side wall of the driving disc (646).
9. The AI intelligent adipose-derived stem cell culturing method according to claim 1, wherein a hopper (2 a) is fixed to the top of the feed inlet (2), and the hopper (2 a) is arranged in a shape of a large top and a small bottom.
10. The AI intelligent adipose-derived stem cell culturing method according to claim 1, wherein the lower end of the outer wall of the tank body (1) is equidistantly fixed with support rods (7), and the bottom of the support rods (7) is fixed with a support base (8).
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116002797A (en) * | 2023-03-23 | 2023-04-25 | 胜利油田临海石油科技有限责任公司 | Device and method for recycling organic solvent from wastewater |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116002797A (en) * | 2023-03-23 | 2023-04-25 | 胜利油田临海石油科技有限责任公司 | Device and method for recycling organic solvent from wastewater |
| CN116002797B (en) * | 2023-03-23 | 2023-08-18 | 胜利油田临海石油科技有限责任公司 | Device and method for recycling organic solvent from wastewater |
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