CN109988700A - A kind of stem cell separator - Google Patents
A kind of stem cell separator Download PDFInfo
- Publication number
- CN109988700A CN109988700A CN201711476496.XA CN201711476496A CN109988700A CN 109988700 A CN109988700 A CN 109988700A CN 201711476496 A CN201711476496 A CN 201711476496A CN 109988700 A CN109988700 A CN 109988700A
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- China
- Prior art keywords
- stem cell
- tank body
- taper
- cavity
- collection portion
- Prior art date
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 119
- 238000002347 injection Methods 0.000 claims abstract description 33
- 239000007924 injection Substances 0.000 claims abstract description 33
- 239000000126 substance Substances 0.000 claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 claims description 35
- 238000010438 heat treatment Methods 0.000 claims description 20
- 238000007789 sealing Methods 0.000 claims description 20
- 238000000926 separation method Methods 0.000 claims description 20
- 238000009434 installation Methods 0.000 claims description 18
- 230000008676 import Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 description 16
- 239000002699 waste material Substances 0.000 description 15
- 239000000463 material Substances 0.000 description 12
- 239000000376 reactant Substances 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 230000009514 concussion Effects 0.000 description 4
- 238000001976 enzyme digestion Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 238000013517 stratification Methods 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 210000003716 mesoderm Anatomy 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 210000003981 ectoderm Anatomy 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Abstract
The present invention provides a kind of stem cell separators, including tank body, it is equipped with taper in tank body and reacts cavity, the top of tank body is equipped with the injection conduit and draw catheter that the closed connection of cavity is reacted with taper, the lower part of tank body, which is provided with, reacts the stem cell collection portion that cavity is connected with taper, and stem cell collection portion and tank body are completed to exchange with external substance by injection conduit and draw catheter；And driving device, driving device are detachably arranged in the bottom wall outer of tank body, driving device is for driving tank body to rotate to be shaken or be centrifugated.The configuration of the present invention is simple, it is low in cost, full-automatic operation and efficiently separating and extracting for stem cell can be precisely controlled.
Description
Technical field
The invention belongs to medicine instrument technical field, specially a kind of stem cell separator.
Background technique
Stem cell refers to the initial cell with self-renewing and multi-lineage potential, is the cells of origin of body, in spy
The cell as one or more composition tissues or organ can be broken up under conditions of fixed, it is widely used in preclinical medicine and grinds
Study carefully.Self-renewing refers to that stem cell can maintain the size of stem cell population by cell division；Multidirectional Differentiation refers to stem cell
With plasticity, different histocytes can be divided into different environments to constitute the organizer of the various complexity of body
Official.
Mescenchymal stem cell (MSCs) is one of stem cell, from the mesoderm and ectoderm of mesoderm growing early stage, because
It has the characteristics that multi-lineage potential, hematopoiesis support and promote stem cell implantation, immunoregulation and self-replacation and increasingly by
To the concern of people.Constantly seek and improve MSCs acquisition source and method be further investigation and utilization MSCs necessity before
It mentions, the separation of MSCs at present and culture lack unified approach, and common separating and extracting process is to use tissue block to originally culture
Method or enzyme digestion obtain mesenchymal stem cells.
And the separation and Extraction process of mescenchymal stem cell is mostly manual operation at present, the degree of automation is low, separation step
It is rapid to be reacted first to inject stem cell stoste, water and enzyme into reaction vessel, extracting solution completion is regathered after complete reaction
Separating step, the process steps are cumbersome, and randomness is big, it is easy to lead to separation because of human operational error not exclusively, time-consuming consumption
Power；There is also some stem cell extract equipments at present, but these device structures are complicated, volume is larger, expensive, are not suitable for
It is promoted and applied in clinical medicine.
Summary of the invention
The technical problem to be solved in the present invention is that defect in the prior art is overcome, to provide a kind of stem cell separation
Device, the stem cell separator structure is simple, low in cost, full-automatic operation and the height that can be precisely controlled stem cell
Effect separation and extraction.
In order to achieve the above objectives, the present invention adopts the following technical scheme:
A kind of stem cell separator, comprising:
Tank body, the interior taper that is equipped with of the tank body react cavity, and the top of the tank body, which is equipped with, reacts cavity with the taper
The injection conduit and draw catheter of closed connection, the lower part of the tank body are provided with react that cavity is connected with the taper dry
Cell collection portion, the stem cell collection portion and the tank body are completed and the external world by the injection conduit and the draw catheter
The exchange of substance；And
Driving device, the driving device are detachably arranged in the bottom wall outer of the tank body, and the driving device is used
In the drive tank body rotation to be shaken or be centrifugated.
Preferably: the cross section of the taper reaction cavity is ellipse.
Further: the stem cell collection portion is two, and two stem cell collection portions are relatively set to the cone
The long axis both ends of shaped reaction cavity.
Further: the lower part of the tank body, which is outward extended with, reacts the guide pipe that cavity is connected with the taper,
The guide pipe is detachably connected with the stem cell collection portion, and the guide pipe is used to install the stem cell collection portion and will
Substance in the taper reaction cavity imports in the stem cell collection portion.
Yet further: the guide pipe is arranged obliquely.
Yet further: the guide pipe is threadedly coupled with the stem cell collection portion.
Further: the stem cell collection portion and the tank body are integrally formed, the stem cell collection portion with it is described
The material of tank body is resin.
Preferably: being provided with guide protrusions on the bottom wall in the tank body for constituting the taper reaction cavity.
Preferably: the junction of the injection conduit and the draw catheter and the tank body is provided with sealing dress
It sets, the sealing device makes the injection conduit and the draw catheter react cavity dynamic sealing with the taper.
Preferably: the bottom of the tank body is equipped with installation protrusion, offers and the driving device in the installation protrusion
The mounting groove that output shaft matches, the driving device realized and then the output shaft is installed in the mounting groove with
The tank body is detachably connected.
Preferably: the stem cell separator further includes heating apparatus and controller, and the heating apparatus is used for institute
It states tank body and carries out heated at constant temperature, the controller is connect with the heating apparatus and the driving device signal respectively, the control
Device processed is used to control the heated condition of the heating apparatus and the operating status for controlling the driving device.
The invention has the benefit that
1, stem cell separator provided by the invention includes tank body and driving device, and tank body is used for containing stem cell
Raw material is digested and is separated, and driving device is for being centrifuged the reactant of tank body, and wherein tank body lower part is provided with
Stem cell collection portion, and stem cell collection portion reacts cavity with the taper of tank interior and is connected and the reaction of composition collection and is collected in one
The accommodating space of body, which only passes through the injection conduit that top of the tank is arranged in and draw catheter is completed and external substance
Exchange, therefore entire tank body is a sealable device, and injection conduit is used for the injection into accommodating space and contains stem cell
The reactive materials such as raw material, water, enzyme, after the completion of reactive material digests in accommodating space, it is high that driving device drives tank body to carry out
Speed rotation, so that reaction product is centrifugated, stem cell reacts the side of cavity along taper during centrifuge separation
Wall falls into the stem cell collection portion that tank body lower part is arranged in, and the draw catheter that cavity is connected finally is reacted with taper and passes through power
Pump draws away the waste liquid after enzymatic hydrolysis, and entire separation and Extraction process all carries out under closed environment, avoids sample and the external world
The contacts such as driving device or control device cause pollution of the microorganism to stem cell, and then realize the safety asepsis point of stem cell
From, since tank body is at low cost, and both can be used as reaction vessel can also be installed directly on the output shaft of driving device constitute from
Center device is centrifuged it is not necessary that liquid to be transferred in other centrifugal devices, therefore not only easy to use but also can be used as primary
Property consumptive material convenient for secondary replacement use, i.e., it is every to carry out stem cell and extract directly replacing tank body and being connected with tank body
Injection conduit and draw catheter, efficiently avoid avoiding the cross contamination between different samples；
Reaction cavity is set as taper both and can have biggish reaction compartment, but also has preferable airtightness, keeps away
Exempt from reactant spluttering during centrifugation, while side wall has certain slope, reactant is fallen back to more easily along side wall
Bottom, layering are also easier to；
Enzymatic hydrolysis can be made to separate in reactant in the bottom connection stem cell collection portion of taper reaction cavity dry
Cell is deposited solely in together by centrifugal action, and is not easy to mix with waste liquid, to release the limit to draw catheter position
Draw catheter need to be only fixed on tank body by system, and the bottom end of draw catheter is disposed proximate to the bottom of taper reaction cavity
, the purpose of full automatic high efficiency separation is further realized, separates the present invention preferably with waste liquid, avoids generating dry thin
The problem that born of the same parents are removed when waste liquid is drawn, separation and Extraction effect are good；
Integral device of the present invention is simply light, at low cost, and operating process is full-automatic, avoids many and diverse separation step of tradition
Suddenly；
2, the cross section of taper reaction cavity is set as ellipse, then reacting cavity is elliptical cone, and elliptical cone is most
At major diameter, centrifugal force is maximum, and the long axis both ends that stem cell collection portion is reacted to cavity with taper at this time are connected, when driving fills
When setting drive tank body centrifuge separation, taper reacts the direct targetedly quilt under the guiding role of its side wall of the stem cell in cavity
Assemble the concentration that the present apparatus is greatly increased into stem cell collection portion, to realize efficiently separating for stem cell；
3, there are two types of form, one is empty corresponding to taper reaction for the connection of stem cell collection portion and tank body in the present invention
Guide pipe is respectively set in the tank body lower part at chamber long axis both ends, and the guide pipe and tank body are integrally formed, and is extended naturally by tank body lower part
Out, it can be not only used for being detachably connected with stem cell collection portion (as being threadedly coupled), and can be more smoothly by cone
Substance in shaped reaction cavity is imported into stem cell collection portion, and the setting of guide pipe can be horizontally disposed or dip down
Tiltedly setting, guide pipe are arranged so that the stem cell after being separated very easily enters in stem cell collection portion, to increase
The mode for having added separative efficiency of the invention, and being detachably connected with stem cell collection portion be convenient for pipetting collect it is dry thin
Born of the same parents；As another enforceable mode, it can be not provided with guide pipe, be directly integrally formed stem cell collection portion and tank body,
It is possible thereby to enhance leakproofness and controllability of the invention, the pollution risk of the present apparatus is reduced, at this time stem cell collection portion and tank
The material of body selects resin escapable cost, and after the completion of centrifuge separation convenient for by stem cell collection portion from medical disposable material
Tank body on cut, can be completed and subsequent the programs such as pipette, store；
4, guide protrusions are provided on the bottom wall in tank body of the present invention for constituting taper reaction cavity, guide protrusions can be with
For two raised lines being symmetrically disposed on bottom wall long axis, when driving device at runtime, raised line can play the role of stirring,
The concussion for being conducive to stem cell mixes and centrifugation layering；
5, whole device of the present invention is salable state, and the exchange of substance is only by injection conduit and draw catheter come real
It is existing, therefore can be very good to avoid outside contamination, due to needing driving device that tank body is driven to be shaken and be centrifuged, the present invention exists
The junction of injection conduit and draw catheter and tank body is provided with sealing device, so that injection conduit, draw catheter
Dynamic sealing is formed between tank body, specifically, which includes seal ring bracket and the axle bed on tank body, injection
Conduit or draw catheter pass through the axis hole on axle bed, axle bed sealing ring built in axis hole, axle bed sealing ring and injection conduit or absorption
Conduit forms cooperation, in addition injects in the axial direction of conduit or draw catheter and also sets up Simmer ring, Simmer ring bracket, sealing
Coil support is mounted on axle bed, and baffle ring is equipped between Simmer ring and axle bed sealing ring, and baffle ring inner wall is equipped with bulge loop, in gear
Sealing ring top, baffle ring inner wall, bulge loop, injection conduit or draw catheter below ring surround accommodating cavity；
6, the connection type between inventive drive means and tank body are as follows: it is equipped with installation protrusion in the bottom of tank body,
The mounting groove to match with driving device output shaft is offered in installation protrusion, driving device is by corresponding to installation for output shaft
Slot carries out installation and is detachably connected to realize with the tank body, of course for further increasing between driving device and tank body
Stability, can also set output shaft to the double-layer structure with inner core and shell, and the shape of inner core and mounting groove matches,
The accommodating cavity formed between shell and inner core and the peripheral wall of installation protrusion match；When installation, inner core is placed in mounting groove, shell
It is wrapped on the outer wall of installation protrusion, is fastenedly connected so as to realize；
7, the present invention is also provided with heating apparatus and controller, effect of the heating apparatus in controller in the bottom of tank body
Under heated at constant temperature can be carried out to tank body, so that reactant is sufficiently reacted, controller also can control the operation shape of driving device simultaneously
State to ensure that the optimal reactive temperature and centrifuge speeds of enzyme digestion reaction, and then improves stem cell separator
Separating effect.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is the structural schematic diagram of one embodiment of the invention；
Fig. 2 is tank body and the perspective view in stem cell collection portion 1 in the above embodiment of the present invention；
Fig. 3 is tank body and the perspective view in stem cell collection portion 2 in the above embodiment of the present invention；
Fig. 4 is tank body and the top view in stem cell collection portion in the above embodiment of the present invention；
Fig. 5 is tank body and the bottom view in stem cell collection portion in the above embodiment of the present invention.
Description of symbols:
1, tank body；11, taper reacts cavity；12, guide protrusions；13, installation protrusion；14, guide pipe；131, mounting groove；
2, conduit is injected；3, draw catheter；4, stem cell collection portion；5, driving device；6, interior room is reacted；61, filter hole；7, heating dress
It sets；8, controller.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
Personnel's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" be based on the orientation or positional relationship shown in the drawings, merely to
Convenient for description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation,
It is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood at this with concrete condition
Concrete meaning in invention.
As long as in addition, the non-structure each other of technical characteristic involved in invention described below different embodiments
It can be combined with each other at conflict.
As shown in Figure 1, the present invention provides a kind of stem cell separator, including tank body 1, tank body 1 are interior anti-equipped with taper
Cavity 11 is answered, the top of tank body 1 is equipped with injection conduit 2 and draw catheter 3 that the closed connection of cavity 11 is reacted with taper, tank body 1
Lower part be provided with and react the stem cell collection portion 4 that cavity 11 is connected with taper, stem cell collection portion 4 and tank body 1 pass through note
Enter the material transfer between conduit 2 and the completion of draw catheter 3 and other component；And driving device 5, driving device 5 are detachable
The bottom wall outer for being set to tank body 1, driving device 5 for drive tank body 1 rotation to be shaken or be centrifugated.
Stem cell separator provided by the invention includes tank body 1 and driving device 5, and tank body 1 is used for containing stem cell
Raw material digested and separated, driving device 5 is for being centrifuged the reactant of tank body 1, the course of work are as follows: first
It first passes through injection conduit 2 and the stoste containing stem cell tissue successively is added into the taper of tank body 1 reaction cavity 11, open driving
Device 5 carries out reciprocating rotation to tank body 1 and generates the stoste progress physical separation shaken and made containing stem cell tissue, stratification
Afterwards, waste liquid is exchanged to free surrounding space by draw catheter 3, cavity 11 is then reacted to the taper of tank body 1 by injection conduit 2
Interior plus water repeatedly shaken, cleaned, stratification, and the upper layer waste liquid after cleaning layering is moved to extraneous empty by draw catheter 3
Between, it is chemically reacted finally by injection conduit 2 is enzyme into the taper of tank body 1 reaction cavity 11, during reaction,
Driving device 5 is opened, reciprocating rotation concussion is carried out to tank body 1 and is allowed to sufficiently react, after the reaction was completed the fortune of switching driving device 5
Row mode, and then be centrifuged at a high speed to tank body 1, stem cell is entered along the side wall of tank body 1 by isolated stem cell
In collection portion 4, after stratification, under the action of draw catheter 3, waste liquid is successively moved, and is most removed afterwards through cleaning is repeated several times
After waste liquid, the stem cell being gathered in stem cell collection portion 4 can be obtained.
1 lower part of tank body of the invention is provided with stem cell collection portion 4, and stem cell collection portion 4, which is one, has accommodating cavity
Container, which reacts cavity 11 with the taper inside tank body 1 and is connected composition collection reaction and to be collected in integrated accommodating empty
Between, which passes through the injection conduit 2 that 1 top of tank body is arranged in and the completion of draw catheter 3 and the material between other component
Transhipment, therefore entire tank body 1 is a sealable device, injection conduit 2 is used for the injection into accommodating space and contains stem cell
The reactive materials such as raw material, water, enzyme, after the completion of reactive material digests in accommodating space, driving device 5 drives tank body 1 to carry out
High speed rotation, so that reaction product is centrifugated, stem cell reacts cavity 11 along taper during centrifuge separation
Side wall fall into the stem cell collection portion 4 that 1 lower part of tank body is set, the draw catheter that cavity 11 is connected finally is reacted with taper
3 are drawn away the waste liquid after enzymatic hydrolysis by kinetic pump, and entire separation and Extraction process all carries out under closed environment, effectively avoids
The pollution of fungi and bacterium；Wherein reaction cavity, which is set as taper both, can have biggish reaction compartment, but also have
Preferable airtightness avoids reactant spluttering during centrifugation, while side wall has certain slope, and reactant is easier
Bottom is fallen back to along side wall, layering is also easier to；It can make in the bottom connection stem cell collection portion 4 of taper reaction cavity 11
It obtains the stem cell for digesting and separating in reactant to be deposited solely in together by centrifugal action, and is not easy to mix with waste liquid,
To release limitation to 3 position of draw catheter, draw catheter 3 need to be only fixed on tank body 1, and by the bottom of draw catheter 3
End is disposed proximate to the bottom of taper reaction cavity 11, further realizes the purpose of full automatic high efficiency separation, makes the present invention
It is preferably separated with waste liquid, avoids leading to the problem of what stem cell was removed when waste liquid is drawn, separation and Extraction effect
Fruit is good；Integral device of the present invention is simply light, at low cost, and operating process is full-automatic, avoids many and diverse separating step of tradition.
Wherein injection conduit 2 pass through tank body 1 top one end react with taper cavity 11 be connected to, the other end with fill water,
Enzyme, the reservoir containing stem cell tissue stoste；Cavity 11 is reacted with taper in top one end that draw catheter 3 passes through tank body 1
Connection, the end at the end extend to 1 bottom of tank body, and convenient for more drawing waste liquid, and position is not necessarily to carry out manual once fixing
Adjustment, usual distance of the end away from 1 bottom wall of tank body are not more than 0.5mm, and the other end is sequentially connected kinetic pump and waste liquid is held
Container, whole device of the present invention are salable state, and the exchange of substance is only realized by injection conduit 2 and draw catheter 3, because
This can be very good to be avoided the cross contamination between cell sample, due to need driving device 5 drive tank body 1 carry out concussion and from
The heart, the present invention are provided with sealing device in the junction of injection conduit 2 and draw catheter 3 and tank body 1 and (do not show in figure
Out), so that forming dynamic sealing between injection conduit 2, draw catheter 3 and tank body 1, specifically, which includes
Seal ring bracket and the axle bed on tank body 1 inject the axis hole of conduit 2 or draw catheter 3 on axle bed, axis built in axis hole
Seat sealing ring, axle bed sealing ring and injection conduit 2 or draw catheter 3, which are formed, to be cooperated, and conduit 2 or draw catheter 3 are in addition injected
Simmer ring, Simmer ring bracket are also set up in axial direction, Simmer ring bracket is mounted on axle bed, and Simmer ring and axle bed seal
Baffle ring is equipped between circle, baffle ring inner wall is equipped with bulge loop, sealing ring top, baffle ring inner wall below baffle ring, bulge loop, injection
Conduit 2 or draw catheter 3 surround accommodating cavity；The setting of sealing device can make to inject conduit 2 and draw catheter 3 and tank body 1
Between form dynamic sealing, guarantee the closure of taper reaction cavity 11, avoid pollution and mixed under high speed centrifugation state
Close the spillage of object.
As shown in Fig. 2 or 4, the cross section that taper reacts cavity 11 is ellipse, and it is elliptical cone that taper, which reacts cavity 11,
The long axis both ends that cavity 11 is reacted in stem cell collection portion 4 with taper at this time are connected, when driving device 5 drives the centrifugation point of tank body 1
From when, taper reaction cavity 11 in stem cell under the guiding role of its side wall directly be targetedly aggregated into stem cell receipts
Collection portion 4, greatly increases the concentration of the present apparatus, to realize efficiently separating for stem cell；As shown in Figure 1, being used for structure in tank body 1
Guide protrusions 12 are provided on the bottom wall of tapered reaction cavity 11, guide protrusions 12 can be to be symmetrically disposed on bottom wall long axis
On two raised lines, when driving device 5 at runtime, raised line can play the role of stirring, and the concussion for being conducive to stem cell is mixed
Uniform centrifugation layering.
As illustrated in fig. 1 and 2, there are two types of form, one is right for the connection of stem cell collection portion 4 and tank body 1 in the present invention
Guide pipe 14 should be respectively set in 1 lower part of tank body that taper reacts 11 long axis both ends of cavity, the guide pipe 14 and the one of tank body 1 at
Type is extended out naturally by 1 lower part of tank body, can be not only used for being detachably connected with stem cell collection portion 4 (as being threadedly coupled),
And taper more smoothly can be reacted into the substance in cavity 11 and imported into stem cell collection portion 4, guide pipe 14 is set
Setting can be to be horizontally disposed with or tilting down setting, and guide pipe 14 is arranged so that the stem cell after being separated is very easy to
Enter in stem cell collection portion 4, to increase separative efficiency of the invention, and detachable with stem cell collection portion 4
The mode of connection is convenient for pipetting the stem cell collected；As another enforceable mode, it can be not provided with guide pipe 14,
Directly stem cell collection portion 4 and tank body 1 are integrally formed, it is possible thereby to enhance leakproofness and controllability of the invention, reduce this
The pollution risk of device, the material preferred resin in stem cell collection portion 4 and tank body 1 at this time, escapable cost, and when centrifuge separation
After the completion convenient for cutting stem cell collection portion 4 from the tank body 1 of medical disposable material, it can be completed and subsequent pipette, store
Program, safety and sanitation.
As shown in Fig. 3 or 5, the bottom wall outer of tank body 1 is provided with installation protrusion 13, offers and drives in installation protrusion 13
The mounting groove 131 that 5 output shaft of device matches, driving device 5 can be motor, and motor is by being mounted on mounting groove for output shaft
To complete to be detachably connected with tank body 1 in 131.It is firm between driving device 5 and tank body 1 of course for further increasing
Property, the output shaft of driving device 5 can also be set to the double-layer structure with inner core and shell, the shape of inner core and mounting groove
Match, the accommodating cavity formed between shell and inner core and the peripheral wall of installation protrusion match；When installation, inner core is placed in mounting groove
Interior, shell is wrapped on the outer wall of installation protrusion, is fastenedly connected so as to realize；
Stem cell separator is also provided with heating apparatus 7 and controller 8, and heating apparatus 7 is used to carry out constant temperature to tank body 1
Heating, heating apparatus 7 can be silica gel heating sheet, both can be set in the bottom of tank body 1, can also surround the side wall of tank body 1；
Controller 8 is connect with heating apparatus 7 and 5 signal of driving device respectively, for controlling the heated condition of heating apparatus 7 and being used for
The operating status of driving device 5 is controlled, heating apparatus 7 can carry out heated at constant temperature to tank body 1 under the action of controller, make anti-
Answer object sufficiently to react, for example, when in use by temperature setting be 37-40 DEG C, preferably 37 DEG C, to ensure that enzyme digestion reaction
Optimal reactive temperature, controller 8 also can control the operating status of driving device 5 simultaneously, such as when enzyme digestion reaction, control
Device 8 processed controls driving device 5 and drives tank body 1 to carry out positive and negative rotation so that reaction fullys shake in the substance in tank body 1, works as portion
After dividing waste liquid to be partially removed, controller 8 controls driving device 5 and runs at high speed, to stem cell is centrifugated completely to dry
In cell collection portion 4, the separating effect of stem cell separator is improved.
The above is only a specific embodiment of the invention, but scope of protection of the present invention is not limited thereto, any to be familiar with
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by those skilled in the art, should all cover
Within protection scope of the present invention.Therefore, protection scope of the present invention should be subject to the protection scope in claims.
Claims (10)
1. a kind of stem cell separator characterized by comprising
Tank body, the interior taper that is equipped with of the tank body react cavity, and the top of the tank body is closed equipped with cavity is reacted with the taper
The injection conduit and draw catheter of connection, the lower part of the tank body, which is provided with, reacts the stem cell that cavity is connected with the taper
Collection portion, the stem cell collection portion and the tank body are by the injection conduit and the draw catheter is completed and external substance
Exchange；And
Driving device, the driving device are detachably arranged in the bottom wall outer of the tank body, and the driving device is used for band
The tank body rotation is moved to be shaken or be centrifugated.
2. stem cell separator according to claim 1, which is characterized in that the cross section of the taper reaction cavity is
Ellipse.
3. stem cell separator according to claim 2, which is characterized in that the stem cell collection portion be two, two
A stem cell collection portion is relatively set to the long axis both ends of the taper reaction cavity.
4. stem cell separator according to claim 2 or 3, which is characterized in that the lower part of the tank body extends outwardly
Have and react the guide pipe that cavity is connected with the taper, the guide pipe is detachably connected with the stem cell collection portion, institute
It states guide pipe and imports the stem cell receipts for fixing the stem cell collection portion and the taper being reacted to the substance in cavity
In collection portion.
5. stem cell separator according to claim 4, which is characterized in that the guide pipe is arranged obliquely.
6. stem cell separator according to claim 4, which is characterized in that the guide pipe and the stem cell collection
Portion is threadedly coupled.
7. stem cell separator according to any one of claim 1-3, which is characterized in that be used for structure in the tank body
Guide protrusions are provided on bottom wall at taper reaction cavity.
8. stem cell separator according to any one of claim 1-3, which is characterized in that the injection conduit and
The junction of the draw catheter and the tank body is provided with sealing device, the sealing device make the injection conduit with
And the draw catheter reacts cavity dynamic sealing with the taper.
9. stem cell separator according to any one of claim 1-3, which is characterized in that the bottom of the tank body is set
There is installation protrusion, the mounting groove to match with the driving device output shaft, the driving dress are offered in the installation protrusion
It sets and is realized by the way that the output shaft is installed in the mounting groove and then and be detachably connected with the tank body.
10. stem cell separator according to any one of claim 1-3, which is characterized in that the stem cell separation
Device further includes heating apparatus and controller, and the heating apparatus is used to carry out heated at constant temperature, the controller to the tank body
It is connect respectively with the heating apparatus and the driving device signal, the controller is used to control the heating of the heating apparatus
State and operating status for controlling the driving device.
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| CN201711476496.XA CN109988700B (en) | 2017-12-29 | 2017-12-29 | Stem cell separation device |
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| CN201711476496.XA CN109988700B (en) | 2017-12-29 | 2017-12-29 | Stem cell separation device |
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| CN109988700B CN109988700B (en) | 2023-11-17 |
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