CN113355231A

CN113355231A - A collection isolated culture integrative device for clinical endometrium stem cell

Info

Publication number: CN113355231A
Application number: CN202110773109.9A
Authority: CN
Inventors: 徐珊; 薛翔; 李晓莉; 张雪; 万秋园
Original assignee: Second Affiliated Hospital School of Medicine of Xian Jiaotong University
Current assignee: Second Affiliated Hospital School of Medicine of Xian Jiaotong University
Priority date: 2021-07-08
Filing date: 2021-07-08
Publication date: 2021-09-07

Abstract

The invention discloses an integrated device for collecting, separating and culturing clinical endometrial stem cells, which comprises a separation control console, a semiconductor refrigerator, a first motor, a first centrifugal chamber, a first filter barrel, a first collection tank, a second motor, a second centrifugal chamber, a second filter barrel, a first liquid storage tank, a third motor, a third centrifugal chamber, a third filter barrel, a second collection tank, a fourth motor, a fourth centrifugal chamber, a fourth filter barrel, a second liquid storage tank, a first suction pump, a connecting hose, a collecting handle, a micro motor, a shaft lever, a rotary-cut sheet, a culture sleeve, a heater and a culture tank, wherein the collection device can improve the collection amount and the cell activity rate of endometrium and greatly reduce the pollution probability in the collection process, and the device can be operated conveniently by virtue of an integrated structure, thereby realizing electronic automation and quantitative regulation and control, and a multi-component separation culture mode is utilized, can also enhance the migration ability of cells, so that the cells have multidirectional differentiation potential, and finally achieve the aim of rapid amplification in vitro.

Description

A collection isolated culture integrative device for clinical endometrium stem cell

Technical Field

The invention relates to the technical field of medical instruments, in particular to an integrated device for collecting, separating and culturing clinical endometrial stem cells.

Background

In recent years, researchers have discovered that hematopoietic stem cells are capable of forming certain specialized heart cells that may be used throughout the day to treat and repair weakened or damaged hearts. Research data show that the number of stem cells obtained from menstrual blood is about 30 times that of stem cells extracted from bone marrow, and the stem cells can be used for treating various diseases such as premature ovarian failure, muscular dystrophy, heart diseases and the like after being cultured, so the menstrual blood can become the largest stem cell source in the world.

Modern science finds that women have a unique stem cell, namely endometrial stem cells, in monthly menstrual blood, and the number and the activity of the endometrial stem cells are more than 30 times of those of stem cells from autologous bone marrow. Endometrial stem cells are stem cells with high proliferative capacity found in menstrual blood of female menstrual periods. It has the potential of multidirectional differentiation, such as osteogenic differentiation, odontoblastic differentiation, differentiation into cardiovascular cells and hepatic cells. The success rate of stem cells collected from menstrual blood is 100 times higher than that of bone marrow stem cells. Regenerative medicine requires seed cells with extremely strong dryness, and female uterus is soil for regenerating fresh cells.

According to the above, currently, when collecting, separating and culturing the endometrial stem cells in clinic, because there is no collection, separation and culture integrated device for the endometrial stem cells in the prior art, in the actual operation process, there are many defects and shortcomings, specifically as follows;

1. the steps are complicated, the process is complicated, time and labor are wasted, repeated centrifugal separation operation is needed manually, the labor intensity and time are increased, and the cost is increased.

2. Because a plurality of processes such as collection, separation and culture cannot be intensively completed in one set of equipment, the interference of the external environment is easily caused, and the culture effect of the endometrial stem cells is greatly influenced.

3. The method cannot realize electronization and automation, lacks quantitative regulation and control, has great randomness of manual operation, and hinders the culture and application of endometrial stem cells on a larger scale.

Therefore, in view of the above disadvantages, it is necessary to design an integrated device for collecting, separating and culturing clinical endometrial stem cells.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: provides an integrated device for collecting, separating and culturing clinical endometrial stem cells, and solves the problems in the background art.

In order to solve the technical problems, the technical scheme of the invention is as follows: a collection, separation and culture integrated device for clinical endometrial stem cells comprises a separation control console, a semiconductor refrigerator, a first motor, a first centrifugal chamber, a first filter barrel, a first collection tank, a second motor, a second centrifugal chamber, a second filter barrel, a first liquid storage tank, a third motor, a third centrifugal chamber, a third filter barrel, a second collection tank, a fourth motor, a fourth centrifugal chamber, a fourth filter barrel, a second liquid storage tank, a first suction pump, a connecting hose, a collection handle, a micro motor, a shaft lever, a rotary-cut sheet, a culture sleeve, a heater and a culture tank, wherein the semiconductor refrigerator is fixedly arranged at the rear end of the separation control console, the semiconductor refrigerator is connected with the separation control console through bolts, the first motor is fixedly arranged at the lower end of the left side inside the separation control console, the first motor is connected with the separation control console through bolts, the first centrifugal chamber is positioned at the middle end of the left side inside the separation control console, the first centrifugal chamber is a cylindrical cavity, the first filter barrel is positioned in the first centrifugal chamber, the first filter barrel is rotatably connected with the first centrifugal chamber, the first filter barrel is connected with the first motor through a coupling, the first collection tank is fixedly arranged at the upper end of the left side of the separation control console, the first collection tank is connected with the separation control console through a bolt, the second motor is fixedly arranged at the lower side of the front end in the separation control console, the second motor is connected with the separation control console through a bolt, the second centrifugal chamber is positioned at the middle end of the front side in the separation control console, the second centrifugal chamber is a cylindrical cavity, the second filter barrel is positioned in the second centrifugal chamber, the second filter barrel is rotatably connected with the second centrifugal chamber, the second filter barrel is connected with the second motor through a coupling, and the first liquid storage tank is fixedly arranged at the upper end of the front side of the separation control console, the first liquid storage tank is connected with the separation console through a bolt, the third motor is fixedly arranged at the lower side of the rear end in the separation console, the third motor is connected with the separation console through a bolt, the third centrifugal chamber is arranged at the middle end of the rear side in the separation console, the third centrifugal chamber is a cylindrical cavity, the third filter barrel is arranged in the third centrifugal chamber, the third filter barrel is rotatably connected with the third centrifugal chamber, the third filter barrel is connected with the third motor through a coupling, the second collection tank is fixedly arranged at the upper end of the rear side in the separation console, the second collection tank is connected with the separation console through a bolt, the fourth motor is fixedly arranged at the lower end of the right side in the separation console, the fourth motor is connected with the separation console through a bolt, and the fourth centrifugal chamber is arranged at the middle end of the right side in the separation console, the fourth centrifugal chamber is a cylindrical cavity, the fourth filter vat is positioned in the fourth centrifugal chamber, the fourth filter vat is rotatably connected with the fourth centrifugal chamber, the fourth filter vat is connected with the fourth motor through a coupler, the second liquid storage tank is fixedly arranged at the upper end of the right side in the separation control console, the second liquid storage tank is connected with the separation control console through a bolt, the first suction pump is fixedly arranged at the upper end of the left side of the separation control console, the first suction pump is connected with the separation control console through a bolt, the connecting hose is fixedly arranged at the left side of the first suction pump, the connecting hose is connected with the first suction pump through hot melting, the acquisition handle is fixedly arranged at the left side of the connecting hose, the acquisition handle is connected with the connecting hose through internal and external threads, the micro motor is fixedly arranged at the lower end of the right side in the acquisition handle, and the micro motor is connected with the acquisition handle through hot melting, the shaft lever set firmly in the micro motor left side, shaft lever and micro motor adopt the coupling joint, and the shaft lever with gather the handle and adopt and rotate the connection, rotary-cut piece set firmly in shaft lever left side port, rotary-cut piece and shaft lever adopt the tight fit to be connected, and rotary-cut piece and gather the handle and adopt and rotate the connection, the culture cover set firmly in separation control cabinet right side, culture cover and separation control cabinet adopt bolted connection, the heater set firmly in culture cover inside bottom, heater and culture cover adopt bolted connection, the culture tank place inside the culture cover, culture tank and culture cover adopt swing joint.

Further, separation control cabinet right side still set firmly the honeycomb duct, honeycomb duct and separation control cabinet adopt the hot melt to be connected, just honeycomb duct and fourth centrifugal chamber adopt the hot melt to be connected, the honeycomb duct upper end still set firmly the second suction pump, second suction pump and honeycomb duct adopt the hot melt to be connected, separation control cabinet front end still set firmly control panel, control panel and separation control cabinet adopt bolted connection, just control panel respectively with semiconductor refrigerator, first motor, second motor, third motor, fourth motor, first suction pump, micro motor and second suction pump adopt the signal of telecommunication line connection, separation control cabinet rear end upper side still set firmly handrail frame, handrail frame and separation control cabinet adopt bolted connection.

Further, first solenoid valve has still set firmly between first centrifugal chamber and the second centrifugal chamber, first solenoid valve adopt the hot melt to be connected with first centrifugal chamber and second centrifugal chamber respectively, just first solenoid valve and control panel adopt the signal of telecommunication line to be connected, first centrifugal chamber and first collection tank between still set firmly first imbibition pump, first imbibition pump adopt the hot melt to be connected with first centrifugal chamber and first collection tank respectively, just first imbibition pump and control panel adopt the signal of telecommunication line to be connected.

Further, a second electromagnetic valve is fixedly arranged between the second centrifugal chamber and the third centrifugal chamber, the second electromagnetic valve is respectively connected with the second centrifugal chamber and the third centrifugal chamber in a hot melting mode, the second electromagnetic valve is connected with the control panel in an electric signal line mode, a first liquid injection pump is fixedly arranged between the second centrifugal chamber and the first liquid storage tank, the first liquid injection pump is respectively connected with the second centrifugal chamber and the first liquid storage tank in a hot melting mode, and the first liquid injection pump is connected with the control panel in an electric signal line mode.

Further, a third electromagnetic valve is fixedly arranged between the third centrifugal chamber and the fourth centrifugal chamber, the third electromagnetic valve is respectively connected with the third centrifugal chamber and the fourth centrifugal chamber in a hot melting mode, the third electromagnetic valve is connected with the control panel in an electric signal line mode, a second liquid suction pump is fixedly arranged between the third centrifugal chamber and the second collecting tank, the second liquid suction pump is respectively connected with the third centrifugal chamber and the second collecting tank in a hot melting mode, and the second liquid suction pump is connected with the control panel in an electric signal line mode.

Further, a second liquid injection pump is fixedly arranged between the fourth centrifugal chamber and the second liquid storage tank, the second liquid injection pump is respectively connected with the fourth centrifugal chamber and the second liquid storage tank in a hot melting mode, and the second liquid injection pump is connected with the control panel through an electric signal line.

Further, first accumulator, first liquid jar, second accumulator and second hold liquid tank top still all set firmly sealed apron, sealed apron respectively with first accumulator, first liquid jar, second accumulator and second hold liquid jar and adopt inside and outside threaded connection.

Furthermore, the inside left side of collection handle still set firmly two solid fixed ring sections, two solid fixed ring sections and collection handle adopt the hot melt to be connected.

Furthermore, the front end of the culture sleeve is fixedly provided with a temperature controller, the temperature controller is connected with the culture sleeve by bolts, and the temperature controller is respectively connected with the semiconductor refrigerator and the heater by electric signal lines.

Further, the culture tank top still set firmly the connection lid, connection lid and culture tank adopt inside and outside threaded connection, just connection lid and honeycomb duct adopt inside and outside threaded connection.

Compared with the prior art, the device for collecting, separating and culturing the clinical endometrial stem cells has the following advantages:

1. firstly, the collection amount of endometrium and the survival rate of stem cells in endometrium can be improved through the collection device, and the pollution probability in the collection process of endometrium can be greatly reduced, so that the collection amount of endometrium and the survival rate of stem cells extracted from endometrium are improved.

2. Secondly, with the help of the isolated culture device of integral type, not only can make things convenient for medical personnel to operate, realized electronization, automation and reach the regulation and control of quantization, solved the big problem of the complete manual operation randomness of tradition, with the help of the culture method behind the multiunit centrifugation in addition to can strengthen the migration ability of endometrium stem cell, make it have multidirectional differentiation potential, finally reach the quick amplification purpose in vitro.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a front view of an integrated device for collecting, separating and culturing clinical endometrial stem cells;

FIG. 2 is a top view of an integrated device for collecting, separating and culturing clinical endometrial stem cells;

FIG. 3 is a sectional view of an A-direction of an integrated device for collecting, separating and culturing clinical endometrial stem cells;

FIG. 4 is a B-direction cross section of an integrated device for collecting, separating and culturing clinical endometrial stem cells;

FIG. 5 is a C-direction cross section of an integrated device for collecting, separating and culturing clinical endometrial stem cells;

FIG. 6 is an enlarged cross-sectional view of the harvesting handle portion;

FIG. 7 is an enlarged cross-sectional view of the slicing portion of the double securing ring;

FIG. 8 is a perspective view of an integrated device for collecting, separating and culturing clinical endometrial stem cells, which is shown in FIG. 1;

fig. 9 is a perspective view of an integrated device for collecting, separating and culturing clinical endometrial stem cells, and the device is shown in fig. 2.

A separation control console 1, a semiconductor refrigerator 2, a first motor 3, a first centrifugal chamber 4, a first filter tank 5, a first collection tank 6, a second motor 7, a second centrifugal chamber 8, a second filter tank 9, a first liquid storage tank 10, a third motor 11, a third centrifugal chamber 12, a third filter tank 13, a second collection tank 14, a fourth motor 15, a fourth centrifugal chamber 16, a fourth filter tank 17, a second liquid storage tank 18, a first suction pump 19, a connecting hose 20, a collecting handle 21, a micro motor 22, a shaft rod 23, a rotary-cut sheet 24, a culture sleeve 25, a heater 26, a culture tank 27, a flow guide pipe 101, a second suction pump 102, a control panel 103, an armrest frame 104, a first electromagnetic valve 401, a first liquid suction pump 402, a second electromagnetic valve 801, a first liquid injection pump 802, a third electromagnetic valve 1201, a second liquid suction pump 1202, a second liquid injection pump 1601, a sealing cover plate 1801, a double-slice fixing ring 2101, Temperature controller 2501, connection lid 2701.

The following detailed description will be further described in conjunction with the above-identified drawings.

Detailed Description

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the concepts underlying the described embodiments, however, it will be apparent to one skilled in the art that the described embodiments may be practiced without some or all of these specific details, and in other cases well-known process steps have not been described in detail.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the invention.

As shown in fig. 1, 2, 3, 4, 5, 6, 7, 8, and 9, an integrated device for collection, separation, and culture of clinical endometrial stem cells includes a separation console 1, a semiconductor refrigerator 2, a first motor 3, a first centrifugal chamber 4, a first filter vat 5, a first collection tank 6, a second motor 7, a second centrifugal chamber 8, a second filter vat 9, a first liquid storage tank 10, a third motor 11, a third centrifugal chamber 12, a third filter vat 13, a second collection tank 14, a fourth motor 15, a fourth centrifugal chamber 16, a fourth filter vat 17, a second liquid storage tank 18, a first suction pump 19, a connection hose 20, a collection handle 21, a micro motor 22, a shaft 23, a rotary cutter 24, a culture sleeve 25, a heater 26, and a culture tank 27, wherein the semiconductor refrigerator 2 is fixed to the rear end of the separation console 1, and the semiconductor refrigerator 2 and the separation console 1 are connected by bolts, the first motor 3 is fixedly arranged at the lower end of the left side inside the separation control console 1, the first motor 3 is connected with the separation control console 1 through bolts, the first centrifugal chamber 4 is arranged at the middle end of the left side inside the separation control console 1, the first centrifugal chamber 4 is a cylindrical cavity, the first filter barrel 5 is arranged inside the first centrifugal chamber 4, the first filter barrel 5 is rotatably connected with the first centrifugal chamber 4, the first filter barrel 5 is connected with the first motor 3 through a coupling, the first collection tank 5 is fixedly arranged at the upper end of the left side of the separation control console 1, the first collection tank 6 is connected with the separation control console 1 through bolts, the second motor 7 is fixedly arranged at the lower side of the front end inside the separation control console 1, the second motor 7 is connected with the separation control console 1 through bolts, the second centrifugal chamber 8 is arranged at the middle end of the front side inside the separation control console 1, the second centrifugal chamber 8 is a cylindrical cavity, the second filter vat 9 is positioned in the second centrifugal chamber 8, the second filter vat 9 is rotatably connected with the second centrifugal chamber 8, the second filter vat 9 is connected with the second motor 7 through a coupler, the first liquid storage tank 10 is fixedly arranged at the upper end of the front side of the separation console 1, the first liquid storage tank 10 is connected with the separation console 1 through a bolt, the third motor 11 is fixedly arranged at the lower side of the rear end in the separation console 1, the third motor 11 is connected with the separation console 1 through a bolt, the third centrifugal chamber 12 is positioned at the middle end of the rear side in the separation console 1, the third centrifugal chamber 12 is a cylindrical cavity, the third filter vat 13 is positioned in the third centrifugal chamber 12, the third filter vat 13 is rotatably connected with the third centrifugal chamber 12, and the third filter vat 13 is connected with the third motor 11 through a coupler, the second collecting tank 14 is fixedly arranged at the upper end of the rear side of the separation control console 1, the second collecting tank 14 is connected with the separation control console 1 through bolts, the fourth motor 15 is fixedly arranged at the lower end of the right side in the separation control console 1, the fourth motor 15 is connected with the separation control console 1 through bolts, the fourth centrifugal chamber 16 is arranged at the middle end of the right side in the separation control console 1, the fourth centrifugal chamber 16 is a cylindrical cavity, the fourth filter tank 17 is arranged in the fourth centrifugal chamber 16, the fourth filter tank 17 is rotatably connected with the fourth centrifugal chamber 16, the fourth filter tank 17 is connected with the fourth motor 15 through a coupler, the second liquid storage tank 18 is fixedly arranged at the upper end of the right side in the separation control console 1, the second liquid storage tank 18 is connected with the separation control console 1 through bolts, the first suction pump 19 is fixedly arranged at the upper end of the left side of the separation control console 1, the first suction pump 19 is connected with the separation control console 1 by bolts, the connecting hose 20 is fixedly arranged at the left side of the first suction pump 19, the connecting hose 20 is connected with the first suction pump 19 by hot melting, the collecting handle 21 is fixedly arranged at the left side of the connecting hose 20, the collecting handle 21 is connected with the connecting hose 20 by internal and external threads, the micro motor 22 is fixedly arranged at the lower end of the right side in the collecting handle 21, the micro motor 22 is connected with the collecting handle 21 by hot melting, the shaft rod 23 is fixedly arranged at the left side of the micro motor 22, the shaft rod 23 is connected with the micro motor 22 by a coupler, the shaft rod 23 is rotatably connected with the collecting handle 21, the rotary cutting piece 24 is fixedly arranged at the left side port of the shaft rod 23, the rotary cutting piece 24 is connected with the shaft rod 23 by tight fit, and the rotary cutting piece 24 is rotatably connected with the collecting handle 21, the culture sleeve 25 is fixedly arranged on the right side of the separation control platform 1, the culture sleeve 25 is connected with the separation control platform 1 through bolts, the heater 6 is fixedly arranged at the bottom end inside the culture sleeve 25, the heater 26 is connected with the culture sleeve 25 through bolts, the culture tank 27 is arranged inside the culture sleeve 25, and the culture tank 27 is movably connected with the culture sleeve 25;

the device for collecting, separating and culturing the clinical endometrial stem cells has the following functions;

A. when the cells are collected, the collection handle 21 is butted with the first suction pump 19 in advance, the port on the left side of the collection handle 21 is aligned with the collected menstrual blood, then the first suction pump 19 is started to generate negative pressure suction force, so that the blood is favorably sucked into the collection handle 21, meanwhile, the micro motor 22 can be started by medical personnel, namely, the micro motor 22 drives the shaft rod 23 to drive the rotary slice 24 to rotate, and therefore, the blood is stirred, is fully dispersed, and is favorably used for subsequent centrifugal treatment;

B. when cells are separated, the first centrifugal chamber 4 can remove plasma from the cells and centrifuge the plasma, the plasma is sucked out by the aid of the first collection tank 6, the centrifuged cells are led out by the first filter barrel 5 and led into the second centrifugal chamber 8 to be filtered, then antibiotics are added to the cells to centrifuge the cells again, supernatant is discarded by the second filter barrel 9, the cells are led into the third centrifugal chamber 12 to be resuspended in PBS buffer solution, a leucocyte layer is centrifugally sucked out, the cells pass through the third filter barrel 13 and are led into the fourth centrifugal chamber 16, and then the PBS buffer solution is added to centrifuge the cells to obtain the cultured endometrial stem cells;

C. when the cells are cultured; the fourth centrifugal chamber 16 sends cells which can be cultured into the culture tank 27, and then can regulate and control the temperature inside the culture tank 27 by the mutual matching action of the culture sleeve 25, the semiconductor refrigerator 2 and the heater 26, so that the temperature reaches the optimal temperature environment of the cells, and the cells can be rapidly amplified in vitro;

a guide pipe 101 is fixedly arranged on the right side of the separation control platform 1, the guide pipe 101 is connected with the separation control platform 1 by hot melting, the draft tube 101 is connected with the fourth centrifugal chamber 16 by hot melting, the upper end of the draft tube 101 is also fixedly provided with a second suction pump 102, the second suction pump 102 is connected with the draft tube 101 by hot melting, the front end of the separation control console 1 is also fixedly provided with a control panel 103, the control panel 103 is connected with the separation control console 1 by bolts, and the control panel 103 is respectively connected with the semiconductor refrigerator 2, the first motor 3, the second motor 7, the third motor 11, the fourth motor 15, the first suction pump 19, the micro motor 22 and the second suction pump 102 by adopting electric signal lines, an armrest frame 104 is fixedly arranged on the upper side of the rear end of the separation control platform 1, and the armrest frame 104 is connected with the separation control platform 1 through bolts;

it should be noted that the flow guide tube 101 can guide the completely separated cells in the fourth centrifugal chamber 16 into the culture tank 27 by means of the suction force of the second suction pump 102, which is beneficial to the subsequent culture, the control panel 103 can realize the command control of the internal electrical components, and the control panel 103 is internally provided with the PLC programmable module, the a/D converter and the memory, so that the electric signal transmission conversion and control are realized, the electronic and automatic setting and operation of the medical care personnel are facilitated, the armrest frame 104 can facilitate the medical care personnel to grasp and carry the device, and the convenience of the operation is expanded;

a first electromagnetic valve 401 is fixedly arranged between the first centrifugal chamber 4 and the second centrifugal chamber 8, the first electromagnetic valve 401 is respectively connected with the first centrifugal chamber 4 and the second centrifugal chamber 8 in a hot melting manner, the first electromagnetic valve 401 is connected with the control panel 103 in an electric signal line manner, a first liquid suction pump 402 is fixedly arranged between the first centrifugal chamber 4 and the first collection tank 6, the first liquid suction pump 402 is respectively connected with the first centrifugal chamber 4 and the first collection tank 6 in a hot melting manner, and the first liquid suction pump 402 is connected with the control panel 103 in an electric signal line manner;

the first electromagnetic valve 401 can be opened and closed under the control of the control panel 103, when the first electromagnetic valve is opened, the plasma cells removed from the inside of the first centrifugal chamber 4 can be introduced into the second centrifugal chamber 8, and the first suction pump 402 can suck the plasma in the first filter vat 5 into the first collection tank 6, so as to clean the first filter vat 5;

a second electromagnetic valve 801 is fixedly arranged between the second centrifugal chamber 8 and the third centrifugal chamber 12, the second electromagnetic valve 801 is respectively connected with the second centrifugal chamber 8 and the third centrifugal chamber 12 in a hot melting manner, the second electromagnetic valve 801 is connected with the control panel 103 in an electric signal line, a first liquid injection pump 802 is fixedly arranged between the second centrifugal chamber 8 and the first liquid storage tank 10, the first liquid injection pump 802 is respectively connected with the second centrifugal chamber 8 and the first liquid storage tank 10 in a hot melting manner, and the first liquid injection pump 802 is connected with the control panel 103 in an electric signal line manner;

it should be noted that the second electromagnetic valve 801 can be opened and closed under the control of the control panel 103, when the second electromagnetic valve is opened, the centrifugal cells into which the antibiotics are added to the filtrate in the second centrifugal chamber 8 can be introduced into the third centrifugal chamber 12, and the first liquid injection pump 802 can inject the antibiotics in the first liquid storage tank 10 into the second centrifugal chamber 8, so as to facilitate the mixing reaction with the cells;

a third electromagnetic valve 1201 is fixedly arranged between the third centrifugal chamber 12 and the fourth centrifugal chamber 16, the third electromagnetic valve 1201 is respectively connected with the third centrifugal chamber 12 and the fourth centrifugal chamber 16 by hot melting, the third electromagnetic valve 1201 is connected with the control panel 103 by an electric signal line, a second liquid suction pump 1202 is fixedly arranged between the third centrifugal chamber 12 and the second collection tank 14, the second liquid suction pump 1202 is respectively connected with the third centrifugal chamber 12 and the second collection tank 14 by hot melting, and the second liquid suction pump 1202 is connected with the control panel 103 by an electric signal line;

it should be noted that the third electromagnetic valve 1201 can be opened and closed under the control of the control panel 103, and when the third electromagnetic valve is opened, the centrifuged PBS buffer solution in the third centrifugal chamber 12 can be supplied to the fourth centrifugal chamber 16, and the second liquid suction pump 1202 can suck the centrifuged white membrane layer into the second collection tank 14, so as to clean the inside of the third filter vat 13;

a second liquid injection pump 1601 is fixedly arranged between the fourth centrifugal chamber 16 and the second liquid storage tank 18, the second liquid injection pump 1601 is respectively connected with the fourth centrifugal chamber 16 and the second liquid storage tank 18 by hot melting, and the second liquid injection pump 1601 is connected with the control panel 103 by an electric signal line;

it should be noted that the second liquid injection pump 1601 is capable of injecting the PBS buffer solution in the second liquid storage tank 18 into the fourth centrifugal chamber 16, so as to facilitate the subsequent centrifugal separation process;

sealing cover plates 1801 are fixedly arranged at the tops of the first collecting tank 6, the first liquid storage tank 10, the second collecting tank 14 and the second liquid storage tank 18, and the sealing cover plates 1801 are respectively connected with the first collecting tank 6, the first liquid storage tank 10, the second liquid storage tank 14 and the second liquid storage tank 18 through internal and external threads;

it should be noted that the sealing cover plate 1801 can cover the first collection tank 6, the first liquid storage tank 10, the second collection tank 14, and the second liquid storage tank 18, respectively, so as to facilitate the medical staff to clean the blood plasma or add corresponding diluent, red blood cell lysate, etc. after opening, and facilitate the subsequent separation treatment of endometrial stem cells;

a double-fixing-ring slice 2101 is fixedly arranged on the left side inside the acquisition handle 21, and the double-fixing-ring slice 2102 is connected with the acquisition handle 21 through hot melting;

it should be noted that the double-fixing-ring slice 2101 can be matched with the rotary slice 24 to crush the collected blood tissue, which is not only beneficial to the subsequent separation, but also effectively avoids the problem of blockage inside the collection handle 21;

the front end of the culture sleeve 25 is fixedly provided with a temperature controller 2501, the temperature controller 2501 is connected with the culture sleeve 25 through bolts, and the temperature controller 2501 is respectively connected with the semiconductor refrigerator 2 and the heater 26 through electric signal wires;

it should be noted that the temperature controller 2501 can facilitate the medical staff to regulate and control the refrigerating temperature of the semiconductor refrigerator 2 and the heating temperature of the heater 26, and meet the requirements of different process temperatures for separation and cultivation;

the top of the culture tank 27 is also fixedly provided with a connecting cover 2701, the connecting cover 2701 and the culture tank 27 are connected by internal and external threads, and the connecting cover 2701 and the draft tube 101 are connected by internal and external threads;

the connection cover 2701 can close the culture tank 27 and can be in butt joint with the flow guide pipe 101, which is beneficial for the separated endometrial stem cells to enter the culture tank 27 through the flow guide pipe 101, and finally the culture purpose is realized.

The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.

Claims

1. A collection, separation and culture integrated device for clinical endometrial stem cells is characterized by comprising a separation control console, a semiconductor refrigerator, a first motor, a first centrifugal chamber, a first filter barrel, a first collection tank, a second motor, a second centrifugal chamber, a second filter barrel, a first liquid storage tank, a third motor, a third centrifugal chamber, a third filter barrel, a second collection tank, a fourth motor, a fourth centrifugal chamber, a fourth filter barrel, a second liquid storage tank, a first suction pump, a connecting hose, a collection handle, a micro motor, a shaft lever, a rotary-cut sheet, a culture sleeve, a heater and a culture tank, wherein the semiconductor refrigerator is fixedly arranged at the rear end of the separation control console, the first motor is fixedly arranged at the lower end of the left side inside the separation control console, the first centrifugal chamber is positioned at the middle end of the left side inside the separation control console, the first filter barrel is positioned inside the first centrifugal chamber, the first filter tank is connected with the first motor by a coupler, the first collection tank is fixedly arranged at the upper end of the left side of the separation console, the second motor is fixedly arranged at the lower side of the front end in the separation console, the second centrifugal chamber is arranged at the middle end of the front side in the separation console, the second filter tank is arranged in the second centrifugal chamber, the second filter tank is connected with the second motor by a coupler, the first liquid storage tank is fixedly arranged at the upper end of the front side of the separation console, the third motor is fixedly arranged at the lower side of the rear end in the separation console, the third centrifugal chamber is arranged at the middle end of the rear side in the separation console, the third filter tank is arranged in the third centrifugal chamber, the third filter tank is connected with the third motor by a coupler, the second collection tank is fixedly arranged at the upper end of the rear side of the separation console, and the fourth motor is fixedly arranged at the lower end of the right side in the separation console, the fourth centrifugal chamber is positioned at the middle end of the right side in the separation control console, the fourth filter barrel is positioned in the fourth centrifugal chamber, the fourth filter barrel is connected with a fourth motor by a coupler, the second liquid storage tank is fixedly arranged at the upper end of the right side in the separation control console, the first suction pump is fixedly arranged at the upper end of the left side of the separation control console, the connecting hose is fixedly arranged at the left side of the first suction pump, the acquisition handle is fixedly arranged at the left side of the connecting hose, the micro motor is fixedly arranged at the lower end of the right side in the acquisition handle, the shaft lever is fixedly arranged at the left side of the micro motor, the shaft lever is rotatably connected with the acquisition handle, the rotary cutting piece is fixedly arranged at the port at the left side of the shaft lever, the rotary cutting piece is rotatably connected with the acquisition handle, the culture sleeve is fixedly arranged at the right side of the separation control console, the heater is fixedly arranged at the bottom end of the inner part of the culture sleeve, the culture tank is arranged inside the culture sleeve.

2. The device as claimed in claim 1, wherein a flow guide tube is further fixed to the right side of the separation console, the flow guide tube is connected to the fourth centrifugal chamber by thermal melting, a second suction pump is further fixed to the upper end of the flow guide tube, a control panel is further fixed to the front end of the separation console, the control panel is connected to the semiconductor refrigerator, the first motor, the second motor, the third motor, the fourth motor, the first suction pump, the micro motor and the second suction pump by electrical signal lines, and a handrail frame is further fixed to the upper side of the rear end of the separation console.

3. The device according to claim 1, wherein a first electromagnetic valve is fixedly arranged between the first centrifugal chamber and the second centrifugal chamber, the first electromagnetic valve is connected with the control panel through an electrical signal line, a first suction pump is fixedly arranged between the first centrifugal chamber and the first collection tank, and the first suction pump is connected with the control panel through an electrical signal line.

4. The device as claimed in claim 1, wherein a second electromagnetic valve is fixedly arranged between the second centrifugal chamber and the third centrifugal chamber, the second electromagnetic valve is connected with the control panel through an electric signal line, a first liquid injection pump is fixedly arranged between the second centrifugal chamber and the first liquid storage tank, and the first liquid injection pump is connected with the control panel through an electric signal line.

5. The device according to claim 1, wherein a third solenoid valve is further disposed between the third centrifugal chamber and the fourth centrifugal chamber, the third solenoid valve is connected to the control panel via an electrical signal line, a second liquid suction pump is further disposed between the third centrifugal chamber and the second collection tank, and the second liquid suction pump is connected to the control panel via an electrical signal line.

6. The device as claimed in claim 1, wherein a second infusion pump is further disposed between the fourth centrifugal chamber and the second reservoir, and the second infusion pump is connected to the control panel via an electrical signal line.

7. The device as claimed in claim 1, wherein the first collection tank, the first liquid storage tank, the second collection tank and the second liquid storage tank are all provided with sealing cover plates at top portions thereof.

8. The device as claimed in claim 1, wherein a double-fixing-ring slice is further fixed on the left side inside the collection handle.

9. The device of claim 1, wherein a temperature controller is further mounted at the front end of the culture sheath, and the temperature controller is connected to the semiconductor refrigerator and the heater by electrical signal lines.

10. The device as claimed in claim 2, wherein a connection cover is fixed on the top of the culture tank, and the connection cover and the flow guide tube are connected by internal and external threads.