CN215288820U - Adipose-derived stem cell extraction device - Google Patents
Adipose-derived stem cell extraction device Download PDFInfo
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- CN215288820U CN215288820U CN202120365647.XU CN202120365647U CN215288820U CN 215288820 U CN215288820 U CN 215288820U CN 202120365647 U CN202120365647 U CN 202120365647U CN 215288820 U CN215288820 U CN 215288820U
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Abstract
The utility model discloses an adipose-derived stem cell extraction element, include: the upper end of the tank body is provided with an inlet, the lower end of the tank body is provided with an outlet, and the lower end of the inner cavity of the tank body is provided with a filter screen; the stirring tool is arranged in the tank body; the driving device is connected with the stirring tool and used for providing power for the stirring tool; one end of the first hose is connected with the inlet; one end of the second hose is connected with the outlet; the first peristaltic pump is connected to the first hose; and the second peristaltic pump is connected to the second hose. The device automatically extracts fat, saves manpower, improves preparation efficiency, reduces artificial errors, has controllable equipment parameters, solves the problem of batch difference of manual operation, is carried out in a closed environment, greatly reduces pollution risks, has large one-time treatment capacity, and is suitable for the pharmaceutical industry.
Description
Technical Field
The utility model relates to a biological medicine research and development field, in particular to adipose-derived stem cell extraction element.
Background
Adipose-derived stem cells (ADSCs) are stem cells with the potential for multi-directional differentiation that are isolated from adipose tissue. Adipose tissue is abundant in human body, a large number of adipose-derived stem cells (ADSCs) obtained by liposuction have the potential of self-renewal, proliferation and multidirectional differentiation, can be differentiated into adipocytes, chondrocytes, myocytes, osteoblasts, nerve cells, glial cells and islet cells, can secrete various angiogenesis promoting factors and anti-apoptosis factors to resist inflammation and oxidation, can resist the damage of oxygen free radicals, and is expected to become a stem cell source for repairing damaged tissues and organs.
The preparation process of the adipose-derived stem cells comprises the steps of collection, transportation, primary separation preparation and the like of adipose tissues. The collected adipose tissues are subjected to washing, collagenase digestion, sieving, centrifugation and other processes to obtain a vascular stromal component (SVF), and the SVF is further cultured to obtain the adipose-derived stem cells. The whole preparation process of the adipose-derived stem cells needs to ensure strict sterility and prevent cell contamination.
The existing adipose-derived stem cell extraction methods generally comprise two methods: one is to complete washing, collagenase digestion, sieving, centrifugation and other operations by an artificial preparation method. And the other is to adopt automatic fat stem cell separation equipment which is generally imported equipment, has high equipment cost and small equipment handling capacity and is not suitable for the pharmaceutical industry.
The traditional adipose-derived stem cell preparation method comprises the steps of after fat collection, subpackaging adipose tissues into a container which can be closed by a pipette, adding physiological saline or other washing liquid for vibration cleaning, standing for a few minutes after cleaning, and after the adipose tissues are separated from water, sucking the washing liquid on the lower layer by the pipette, wherein the washing process needs to be repeated for several times, and time and labor are wasted. And after the washing is finished, adding collagenase for vibration digestion, and filtering, centrifuging and washing the digested cell suspension to obtain the SVF.
SUMMERY OF THE UTILITY MODEL
To solve at least one technical problem of the prior art, the present disclosure provides an adipose-derived stem cell extraction device, comprising:
the upper end of the tank body is provided with an inlet, the lower end of the tank body is provided with an outlet, and the lower end of the inner cavity of the tank body is provided with a filter screen;
the stirring tool is arranged in the tank body;
the driving device is connected with the stirring tool and used for providing power for the stirring tool;
one end of the first hose is connected with the inlet;
one end of the second hose is connected with the outlet;
the first peristaltic pump is connected to the first hose; and
and the second peristaltic pump is connected to the second hose.
The beneficial effects of this embodiment are that, transport the adipose tissue motion through first peristaltic pump and second peristaltic pump, stir the internal adipose tissue of jar through drive arrangement drive stirring tool, these actions are all that the device is automatic to be accomplished, have saved the manpower greatly, have improved preparation efficiency, have reduced artificial mistake, and equipment parameter is controllable, has solved manual operation's the different problem between the batch, and whole device goes on under inclosed environment, greatly reduced the pollution risk. The device of the embodiment has large one-time treatment capacity and is suitable for the pharmaceutical industry.
In some embodiments, the stirring tool comprises a stirring shaft and blades disposed on the stirring shaft.
In some embodiments, the driving device comprises a servo motor.
In some embodiments, the apparatus further comprises a PLC controller electrically connected to the driving device, the first peristaltic pump and the second peristaltic pump. For controlling the operation of the drive means, the first peristaltic pump and the second peristaltic pump.
In some embodiments, a human-computer interface is also included. The human-computer interface provides an interface for controlling and monitoring the operation of the drive device, the first peristaltic pump and the second peristaltic pump.
In some embodiments, the canister comprises a body and a filter receiver disposed below the body.
In some embodiments, the body is transparent and the filter receiver is 316L stainless steel.
In some embodiments, the other end of the first hose is divided into three first branches, each of which is provided with a clip.
In some embodiments, the other end of the second hose is divided into two second branches, and each of the second branches is provided with a clamp.
In some embodiments, the mesh size is 40 mesh.
Drawings
Fig. 1 is a schematic structural diagram of an adipose-derived stem cell extraction device according to an embodiment of the present disclosure.
Fig. 2 is an exploded view of a filter receiver and a filter screen of an adipose stem cell extraction device according to an embodiment of the present disclosure.
Fig. 3 is a connection diagram of an adipose stem cell extraction device according to an embodiment of the present disclosure in use.
Description of the symbols:
the device comprises a tank body 1, a stirring tool 2, a driving device 3, a first hose 4, a second hose 5, a first peristaltic pump 6, a second peristaltic pump 7, an inlet 8, an outlet 9, a filter screen 10, a main body 11, a filtering receiver 12, a stirring shaft 13, a blade 14, a collecting bottle 15, a waste liquid barrel 16, a first branch pipe 17, a clamp 18, a second branch pipe 19, a physiological saline bag 20, a digestive enzyme bag 21, a fat bottle 22 and an accommodating cavity 23
Detailed Description
Referring to fig. 1, the adipose-derived stem cell extraction device includes a tank 1, a stirring tool 2, a driving device 3, a first hose 4, a second hose 5, a first peristaltic pump 6, and a second peristaltic pump 7.
Referring to fig. 1 and 2, an inlet 8 is provided at the upper end of the tank 1, an outlet 9 is provided at the lower end of the tank 1, and a filter screen 10 is provided at the lower end of the inner cavity of the tank 1. The inlet 8 and the outlet 9 each have a connection piece of 316L stainless steel for connecting the first hose 4 and the second hose 5, respectively. The can body 1 comprises a main body 11 and a filtering receptacle 12 connected to a lower end of the main body 11, and a filtering net 10 is disposed above the filtering receptacle 12. The body 11 is made of transparent glass, and an experimental operator can observe the state of adipose tissues in the body 11 through the body 11. The filtering net 10 is a 40-mesh stainless steel screen used for filtering the stirred adipose tissues, and the tissues enter the filtering receiver 12 for temporary storage after being filtered. The filter receptacle 12 is designed to receive a quantity of adipose tissue, for example, the filter receptacle 12 has a receiving cavity 23 therein for receiving adipose tissue, and the top of the receiving cavity 23 is open. The filter receiver 12 is mounted to the lower end of the body 11 by snap-fitting. The outlet 9 opens at the bottom of the filter receptacle 12.
Referring to fig. 1, the stirring tool 2 includes a stirring shaft 13 and a paddle 14 disposed on the stirring shaft 13. The driving device 3 is arranged above the tank body 1, and specifically comprises a servo motor, which is connected with the stirring tool 2 through a coupler and used for providing power for the stirring tool 2 so as to enable the stirring tool 2 to rotate in the tank body 1 and stir the adipose tissues in the tank body 1.
The first hose 4 and the second hose 5 are disposable medical silicone tubes. One end of the first hose 4 is connected to an inlet 8 for introducing physiological saline, digestive enzymes and adipose tissues into the tank 1. One end of the second hose 5 is connected to an outlet 9 for leading out digested adipose tissue and waste liquid from the tank 1. A first peristaltic pump 6 is connected to the first hose 4 and a second peristaltic pump 7 is connected to the second hose 5. The first peristaltic pump 6 is used for pumping the adipose tissues to the tank body 1 through the first hose 4, and the second peristaltic pump 7 is used for pumping the extracted adipose tissues and the waste liquid to the collecting bottle 15 and the waste liquid barrel 16 through the second hose 5.
Referring to fig. 1, the other end of the first hose 4 is divided into three first branches 17, and each first branch 17 is provided with a clamp 18. The other end of the second hose 5 is divided into two second branches 19, and each second branch 19 is provided with a clip 18.
The adipose-derived stem cell extraction device of the present disclosure further includes a PLC controller (not shown in the figure), and the PLC controller is electrically connected to the driving device 3, the first peristaltic pump 6 and the second peristaltic pump 7, and is configured to control the operation of the driving device 3, the first peristaltic pump 6 and the second peristaltic pump 7. Further, some embodiments of the present disclosure further include a human-computer interface (not shown), such as a touch screen. The human-computer interaction interface is electrically connected with the PLC. The human-machine interface provides an interface for controlling and monitoring the operation of the drive means 3, the first peristaltic pump 6 and the second peristaltic pump 7.
In operation of the adipose-derived stem cell extraction device of the present disclosure, please refer to fig. 3, the three first branch tubes 17 are connected to the saline bag 20, the digestive enzyme bag 21 and the fat bottle 22, respectively, and the two second branch tubes 19 are connected to the collection bottle 15 and the waste liquid barrel 16, respectively. The adipose-derived stem cell extraction device of the present disclosure is operated according to the following steps:
step 1: opening a clamp 18 on a first branch pipe 17 connected with a fat bottle 22, controlling a first peristaltic pump 6 to pump the fat tissue in the fat bottle 22 into the tank body 1, and closing the clamp 18 after the pumping is finished;
step 2: opening a clamp 18 on a first branch pipe 17 connected with a normal saline bag 20, controlling a first peristaltic pump 6 to pump normal saline into the tank body 1, and closing the first peristaltic pump 6 when the normal saline is added to a certain amount;
and step 3: controlling the driving device 3 and the stirring tool 2 to stir the adipose tissues in the tank body 1, opening a clamp 18 on a second branch pipe 19 connected with the waste liquid barrel 16 after the stirring is finished, controlling the second peristaltic pump 7 to pump the stirred and washed waste liquid into the waste liquid barrel 16, and repeating the step for 2 times;
and 4, step 4: opening a clamp 18 on a first branch pipe 17 connected with a digestive enzyme bag 21, controlling a first peristaltic pump 6 to pump digestive enzymes into the tank body 1, closing the first peristaltic pump 6 after the digestive enzymes are added to a specified amount, controlling a driving device 3 and a stirring tool 2 to stir, and stopping stirring after digestion is finished;
and 5: the clamp 18 on the second branch tube 19 connected to the waste liquid tank 16 is closed and the clamp 18 on the second branch tube 19 connected to the collection bottle 15 is opened, and the second peristaltic pump 7 is controlled to pump the digested adipose tissue into the collection bottle 15.
The adipose-derived stem cell extraction device is jointly completed through the first peristaltic pump 6, the second peristaltic pump 7, the stirring tool 2 and the driving device 3, so that the labor is greatly saved, and the artificial errors are reduced. The washing and digestion process of the adipose tissues is carried out in the environment that the servo motor drives the stirring tool 2 to stir, the one-time treatment capacity is large, and the method is suitable for the pharmaceutical industry. The adipose-derived stem cell extraction device has controllable process, improves the preparation efficiency, and solves the problem of batch difference of manual operation. The fat extraction process is carried out in a closed environment, so that the pollution risk is greatly reduced. Compared with import equipment, the adipose-derived stem cell extraction device disclosed by the invention has the advantages that the cost is reduced, and the popularization in the pharmaceutical industry is facilitated.
What has been described above are only some embodiments of the invention. For those skilled in the art, without departing from the inventive concept, several modifications and improvements can be made, which are within the scope of the invention.
Claims (10)
1. Adipose-derived stem cell extraction device, characterized by comprising:
the filter screen is arranged at the lower end of the inner cavity of the tank body;
a stirring tool disposed within the tank body;
the driving device is connected with the stirring tool and used for providing power for the stirring tool;
a first hose having one end connected to the inlet;
a second hose, one end of which is connected with the outlet;
the first peristaltic pump is connected to the first hose; and
and the second peristaltic pump is connected to the second hose.
2. The adipose-derived stem cell extraction device of claim 1, wherein the stirring tool comprises a stirring shaft and a paddle disposed on the stirring shaft.
3. The adipose-derived stem cell extraction device of claim 1, wherein the driving device comprises a servo motor.
4. The adipose-derived stem cell extraction device of claim 1, further comprising a PLC controller electrically connected to the driving device, the first peristaltic pump and the second peristaltic pump.
5. The adipose-derived stem cell extraction device of claim 2, further comprising a human-computer interface.
6. The adipose-derived stem cell extraction device of claim 1, wherein the canister comprises a body and a filter receiver disposed below the body.
7. The adipose-derived stem cell extraction device of claim 6, wherein the body is transparent and the filter receiver is made of 316L stainless steel.
8. The adipose-derived stem cell extraction device of claim 1, wherein the other end of the first hose is divided into three first branches, and each first branch is provided with a clamp.
9. The adipose-derived stem cell extraction device of claim 1, wherein the other end of the second hose is divided into two second branches, and each of the second branches is provided with a clamp.
10. The adipose-derived stem cell extraction device of claim 1, wherein the mesh number of the filter screen is 40 meshes.
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| CN202120365647.XU CN215288820U (en) | 2021-02-07 | 2021-02-07 | Adipose-derived stem cell extraction device |
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| CN202120365647.XU CN215288820U (en) | 2021-02-07 | 2021-02-07 | Adipose-derived stem cell extraction device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058084A (en) * | 2017-02-17 | 2017-08-18 | 广西医科大学 | SVF automatic cell separating devices and its method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058084A (en) * | 2017-02-17 | 2017-08-18 | 广西医科大学 | SVF automatic cell separating devices and its method |
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