CN208055308U - A kind of umbilical cord tissue mescenchymal stem cell separator - Google Patents
A kind of umbilical cord tissue mescenchymal stem cell separator Download PDFInfo
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- CN208055308U CN208055308U CN201721648736.5U CN201721648736U CN208055308U CN 208055308 U CN208055308 U CN 208055308U CN 201721648736 U CN201721648736 U CN 201721648736U CN 208055308 U CN208055308 U CN 208055308U
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- manipulator
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- umbilical cord
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- stem cell
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Abstract
The utility model is related to technical field of cell separation more particularly to a kind of umbilical cord tissue mescenchymal stem cell separators, it includes controller, main body and control door（3）, disengagement chamber（2）It is interior to be equipped with centrifuge tube shelf（4）, culture dish frame（5）, the first culture bottle rack（6）, centrifuge（7）, shaking table（8）, the first manipulator（9）, charger（10）, the second manipulator（11）, liquid getting device（12）, third manipulator（13）, piping and druming mechanism（14）And the 4th manipulator（15）, the culture chamber（1）Inside it is equipped with the second culture bottle rack（16）, this umbilical cord tissue mescenchymal stem cell separator can be automatically separated mescenchymal stem cell.
Description
Technical field
The utility model is related to technical field of cell separation more particularly to a kind of umbilical cord tissue mescenchymal stem cell separation dresses
It sets.
Background technology
Mescenchymal stem cell is one kind of stem cell, embryo's layer in mesoderm growing early stage, has higher differentiation latent
Can, it is widely present in the connective tissues such as the marrow, fat, umbilical cord of human body and organ interstitial, there is self-renewing, height to rise in value
And multi-lineage potential can be divided into fat, bone, cartilage, muscle, cardiac muscle, endothelium etc. in vitro under specific inductive condition
Various Tissues cell still has differentiation potential after continuously cultivating and freeze, and is increasingly becoming pole in organizational project and field of gene
The cell origin having practical value increasingly is valued by people for clinical treatment.
The mescenchymal stem cell of the prior art is typically all to be detached by human hand work, and such mode of operation has
Following disadvantage：1, using manual operation, it is difficult to accurately control the dosage of physiological saline and culture solution；2, the operation ring being manually operated
Border is generally uncontrolled, may influence the mescenchymal stem cell separated in this way；3, using manual operation, thus efficiency compared with
It is low, and it is easy error；4, using manual operation, cannot arbitrarily selected disengaging time, can just be carried out when needing operator
Lock out operation.
Utility model content
Technical problem to be solved in the utility model is：A kind of umbilical cord group that can be automatically separated mescenchymal stem cell is provided
Knit mescenchymal stem cell separator.
Technical solution used by the utility model is：A kind of umbilical cord tissue mescenchymal stem cell separator, it includes
Controller, the main body equipped with culture chamber and disengagement chamber and the control door being arranged between culture chamber and disengagement chamber, in disengagement chamber
Equipped with centrifuge tube shelf, culture dish frame, the first culture bottle rack, centrifuge, shaking table, the first manipulator, charger, the second machinery
Hand, liquid getting device, third manipulator, piping and druming mechanism and the 4th manipulator, culture chamber is interior to be equipped with the second culture bottle rack, and controls
Door processed, charger, liquid getting device, piping and druming mechanism, centrifuge, shaking table, the first manipulator, the second manipulator, third manipulator and
4th manipulator is electrically connected with the controller,
Charger, for as liquid is added in container；
Liquid getting device, for taking out liquid in container or the liquid of taking-up being rejoined back container；
Mechanism is blown and beaten, for blowing and beating the liquid in container；
Centrifuge, for being centrifuged to centrifuge tube；
Shaking table rocks culture for constant temperature；
First manipulator is moved for capturing container；
Second manipulator, for capture umbilical cord and umbilical cord chopping after particle；
Third manipulator, for cutting umbilical cord；
4th manipulator, for squeezing out the remaining Cord blood of umbilical cord.
It is equipped with environment adjusting machine structure in culture chamber and disengagement chamber, environment adjusting machine structure is electrically connected with the controller.
Charger includes liquid feeding head, multiple Bottles for housing different liquids and for extracting the liquid in Bottle
The actuating unit of body, and multiple Bottles are connected to by switching mechanism with liquid feeding head, switching mechanism and actuating unit with control
Device electrical connection processed.
Liquid getting device includes liquid taking head, waste liquid bottle, switch piece and is connected to liquid taking head for generating the dynamic of forward and reverse suction
Power part, multiple Bottles and waste liquid bottle are connected to by switch piece with liquid taking head, and switch piece and force piece are and controller
Electrical connection.
It is both provided with pipette tips on liquid feeding head and liquid taking head, and is equipped with pipette tips frame in disengagement chamber, and it further includes for more
The 5th manipulator of the pipette tips on liquid feeding head and liquid taking head is changed, the 5th manipulator is electrically connected with the controller.
Using the above structure compared with prior art, the utility model has the following advantages：It is controlled by controller each
Component operation, and then being automatically separated and cultivating for mescenchymal stem cell may be implemented, in this way control are accurate, and separative efficiency compared with
Height, and without limit disengaging time, even if operator not detach automatically.
Regulating mechanism is set, can be very good the environment in control disengagement chamber and culture chamber in this way, such as gas concentration lwevel,
Temperature and humidity etc., so that smaller to impact cell being separately cultured room.
Control liquid feeding head by switching mechanism goes out liquid type, and a liquid feeding head is only needed to can be realized plus difference in this way
The demand of kind class I liquid I occupies small volume, easy to use.
By switch piece come control take liquid after liquid be prepare throw away or reuse, can serve as intermediate mistake in this way
It crosses, operates more convenient succinct.
Provided with pipette tips frame and the 5th manipulator, adding liquid every time in this way or take liquid can be by the 5th manipulator
To replace pipette tips so that experiment is more accurate.
Description of the drawings
Fig. 1 is a kind of structural schematic diagram of umbilical cord tissue mescenchymal stem cell separator of the utility model.
As shown in the figure：1, culture chamber；2, disengagement chamber；3, door is controlled；4, centrifuge tube shelf；5, culture dish frame；6, the first culture
Bottle rack；7, centrifuge；8, shaking table；9, the first manipulator；10, charger；11, the second manipulator；12, liquid getting device；13,
Three manipulators；14, mechanism is blown and beaten；15, the 4th manipulator；16, the second culture bottle rack；17, pipette tips frame；18, the 5th machinery
Hand.
Specific implementation mode
The utility model is described further with specific implementation mode below in conjunction with attached drawing, but the utility model is not only
It is limited to detailed description below.
A kind of umbilical cord tissue mescenchymal stem cell separator, it includes controller, equipped with culture chamber 1 and disengagement chamber 2
Main body and the control door 3 being arranged between culture chamber and disengagement chamber are equipped with centrifuge tube shelf 4, culture dish frame 5, the in disengagement chamber 2
One culture bottle rack 6, centrifuge 7, shaking table 8, the first manipulator 9, charger 10, the second manipulator 11, liquid getting device 12, third
Manipulator 13, piping and druming mechanism 14 and the 4th manipulator 15, culture chamber 1 is interior to be equipped with the second culture bottle rack 16, and controls door
3, charger 10, liquid getting device 12, piping and druming mechanism 14, centrifuge 7, shaking table 8, the first manipulator 9, the second manipulator 11, third machine
Tool hand 13 and the 4th manipulator 15 are electrically connected with the controller,
Charger, for as liquid is added in container；
Liquid getting device, for taking out liquid in container or the liquid of taking-up being rejoined back container；
Mechanism is blown and beaten, for blowing and beating the liquid in container；
Centrifuge, for being centrifuged to centrifuge tube；
Shaking table rocks culture for constant temperature；
First manipulator is moved for capturing container；
Second manipulator, for capture umbilical cord and umbilical cord chopping after particle；
Third manipulator, for cutting umbilical cord；
4th manipulator, for squeezing out the remaining Cord blood of umbilical cord.
It is equipped with environment adjusting machine structure in culture chamber 1 and disengagement chamber 2, environment adjusting machine structure is electrically connected with the controller.
Charger 10 is including liquid feeding head, multiple Bottles for housing different liquids and for extracting in Bottle
The actuating unit of liquid, and multiple Bottles are connected to by switching mechanism with liquid feeding head, switching mechanism and actuating unit with
Controller is electrically connected.
Liquid getting device 12 includes liquid taking head, waste liquid bottle, switch piece and is connected to liquid taking head for generating forward and reverse suction
Force piece, multiple Bottles and waste liquid bottle are connected to by switch piece with liquid taking head, switch piece and force piece with control
Device is electrically connected.
It is both provided with pipette tips on liquid feeding head and liquid taking head, and is equipped with pipette tips frame 17 in disengagement chamber, and it further includes being used for
It replaces liquid feeding head and the 5th manipulator 18 of the pipette tips on liquid taking head, the 5th manipulator 18 is electrically connected with the controller.
A kind of control method of umbilical cord tissue mescenchymal stem cell separator, it includes the following steps：
S1, the centrifuge tube equipped with umbilical cord is placed on centrifuge tube shelf；
S2, the first manipulator capture No. 1 culture dish and are moved to addition 10ml capacity physiological saline under charger, then by 1
Number culture dish is put back on culture dish frame；
S3, the second manipulator are placed into No. 1 culture dish from crawl umbilical cord in the centrifuge tube with umbilical cord and clamp navel
Band one end；
S4, the 4th manipulator squeeze out remaining Cord blood in umbilical cord since umbilical cord one end；
Umbilical cord is moved in No. 2 culture dishes, while clamping umbilical cord by S5, the second manipulator, and third robot work will
Umbilical cord cuts into graininess；
No. 2 culture dishes are moved to addition 10ml capacity physiological saline under charger by S6, the first manipulator, then the first machine
Tool No. 2 culture dishes of hand rolling, clean granular umbilical cord, No. 2 culture dishes are moved under liquid getting device and inhale by the first manipulator later
Walk the liquid in culture dish；
Operation in S7, repetition step S6 2 times；
Particle in No. 2 culture dishes is taken out and is added in No. 1 empty centrifuge tube by S8, the second manipulator, is then moved to
0.2% clostridiopetidase A II of 20ml capacity is added under charger, then No. 1 centrifuge tube is placed into 37 DEG C by the first manipulator, 250rpm
Shaking table digests the 2h times；
S9, the first manipulator remove No. 1 centrifuge tube from shaking table, are moved to taking-up 10ml capacity cell liquid under liquid getting device,
Then No. 1 centrifuge tube is put back to centrifuge tube rack by the first manipulator, No. 2 empty centrifuge tubes is taken out, later with No. 2 centrifuge tubes
It is moved to the 10ml capacity cell liquid for being added under liquid getting device and taking out just now；
S10, the first manipulator, which further take out No. 1 centrifuge tube and be moved under liquid getting device, further takes out 10ml capacity cell liquid, then
No. 1 centrifuge tube is put back to centrifuge tube rack by the first manipulator, takes out No. 3 empty centrifuge tubes, is moved later with No. 3 centrifuge tubes
The 10ml capacity cell liquid taken out just now is added under to liquid getting device；
No. 2 centrifuge tubes and No. 3 centrifuge tubes are moved to addition 40ml capacity under charger by S11, the first manipulator respectively
Then No. 2 centrifuge tubes and No. 3 centrifuge tubes are moved to an off scheming by eluent respectively again, with the rotating speed of 300rpm carry out from
The heart 5min times；
S12, the first manipulator take out No. 2 centrifuge tubes, are moved under liquid getting device the 10ml capacity drawn in No. 2 centrifuge tubes
Then No. 2 centrifuge tubes are placed back into centrifuge tube rack by supernatant, pick up No. 4 empty centrifuge tubes later and be moved under liquid getting device
The 10ml capacity supernatants taken out just now are added, then rock No. 4 centrifuge tubes, and No. 4 centrifuge tubes are moved to an off in scheming；
S13, the first manipulator take out No. 3 centrifuge tubes, are moved under liquid getting device the 10ml capacity drawn in No. 3 centrifuge tubes
Then No. 3 centrifuge tubes are placed back into centrifuge tube rack by supernatant, pick up No. 5 empty centrifuge tubes later and be moved under liquid getting device
The 10ml capacity supernatants taken out just now are added, then rock No. 5 centrifuge tubes, and No. 5 centrifuge tubes are moved to an off in scheming；
S14, centrifuge work, the centrifugation 10min times are carried out with the rotating speed of 2000rpm；
S15, the first manipulator capture No. 4 centrifuge tubes and are moved to taking-up 5ml capacity supernatants under liquid getting device, then move to
10ml capacity eluents are added under charger, is moved to later under piping and druming mechanism and carries out piping and druming 5 times, move again under charger and add
Enter 40ml capacity eluents, later again moves to an off No. 4 centrifuge tubes in scheming；
S16, the first manipulator capture No. 5 centrifuge tubes and are moved to taking-up 5ml capacity supernatants under liquid getting device, then move to
10ml capacity eluents are added under charger, is moved to later under piping and druming mechanism and carries out piping and druming 5 times, move again under charger and add
Enter 40ml capacity eluents, later again moves to an off No. 5 centrifuge tubes in scheming；
S17, centrifuge work, the centrifugation 5min times are carried out with the rotating speed of 2000rpm；
S18, step S15 is repeated once to step S17；
S19, the first manipulator capture No. 4 centrifuge tubes and are moved to addition 1ml capacity culture mediums under charger, move again to and blow
It beats under mechanism and blows and beats 5 times, the cell liquid in No. 4 centrifuge tubes is poured by the first manipulator later is placed on the placement of the first culture bottle
In No. 1 empty culture bottle on frame, and No. 4 centrifuge tubes are placed back into centrifuge tube rack, then the first manipulator captures No. 1
Culture bottle is moved to addition 14ml capacity culture solutions, the first manipulator under charger and rocks culture bottle, and then control control door is beaten
It opens, then No. 1 culture bottle is moved on the second culture bottle rack in culture chamber by the first manipulator, is then shut off control
Door；
S20, the first manipulator capture No. 5 centrifuge tubes and are moved to addition 1ml capacity culture mediums under charger, move again to and blow
It beats under mechanism and blows and beats 5 times, the cell liquid in No. 5 centrifuge tubes is poured by the first manipulator later is placed on the placement of the first culture bottle
In No. 2 empty culture bottles on frame, and No. 5 centrifuge tubes are placed back into centrifuge tube rack, then the first manipulator captures No. 2
Culture bottle is moved to addition 14ml capacity culture solutions, the first manipulator under charger and rocks culture bottle, and then control control door is beaten
It opens, then No. 2 culture bottles are moved on the second culture bottle rack in culture chamber by the first manipulator, are then shut off control
Door.
Claims (5)
1. a kind of umbilical cord tissue mescenchymal stem cell separator, it is characterised in that：It includes controller, is equipped with culture chamber（1）
With disengagement chamber（2）Main body and the control door that is arranged between culture chamber and disengagement chamber（3）, disengagement chamber（2）It is interior to be equipped with centrifugation
Pipe support（4）, culture dish frame（5）, the first culture bottle rack（6）, centrifuge（7）, shaking table（8）, the first manipulator（9）, liquid feeding
Device（10）, the second manipulator（11）, liquid getting device（12）, third manipulator（13）, piping and druming mechanism（14）And the 4th manipulator
（15）, the culture chamber（1）Inside it is equipped with the second culture bottle rack（16）, and the control door（3）, charger（10）, take liquid
Device（12）, piping and druming mechanism（14）, centrifuge（7）, shaking table（8）, the first manipulator（9）, the second manipulator（11）, third manipulator
（13）And the 4th manipulator（15）It is electrically connected with the controller,
The charger, for as liquid is added in container；
Liquid getting device, for taking out liquid in container or the liquid of taking-up being rejoined back container；
Mechanism is blown and beaten, for blowing and beating the liquid in container；
Centrifuge, for being centrifuged to centrifuge tube；
Shaking table rocks culture for constant temperature；
First manipulator is moved for capturing container；
Second manipulator, for capture umbilical cord and umbilical cord chopping after particle；
Third manipulator, for cutting umbilical cord；
4th manipulator, for squeezing out the remaining Cord blood of umbilical cord.
2. a kind of umbilical cord tissue mescenchymal stem cell separator according to claim 1, it is characterised in that：The culture
Chamber（1）With disengagement chamber（2）It is inside equipped with environment adjusting machine structure, the environment adjusting machine structure is electrically connected with the controller.
3. a kind of umbilical cord tissue mescenchymal stem cell separator according to claim 1, it is characterised in that：The liquid feeding
Device（10）Multiple Bottles including liquid feeding head, for housing different liquids and the power for extracting the liquid in Bottle
Mechanism, and the multiple Bottle is connected to by switching mechanism with liquid feeding head, the switching mechanism and actuating unit with control
Device electrical connection processed.
4. a kind of umbilical cord tissue mescenchymal stem cell separator according to claim 1, it is characterised in that：It is described to take liquid
Device（12）It is connected to including liquid taking head, waste liquid bottle, switch piece and with liquid taking head force piece for generating forward and reverse suction, it is described
Multiple Bottles and waste liquid bottle are connected to by switch piece with liquid taking head, and the switch piece and force piece are electric with controller
Connection.
5. a kind of umbilical cord tissue mescenchymal stem cell separator according to claim 3 or 4, it is characterised in that：It is described
It is both provided with pipette tips on liquid feeding head and liquid taking head, and is equipped with pipette tips frame in the disengagement chamber（17）, and it further includes for more
Change the 5th manipulator of the pipette tips on liquid feeding head and liquid taking head（18）, the 5th manipulator（18）It is electrically connected with the controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721648736.5U CN208055308U (en) | 2017-12-01 | 2017-12-01 | A kind of umbilical cord tissue mescenchymal stem cell separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721648736.5U CN208055308U (en) | 2017-12-01 | 2017-12-01 | A kind of umbilical cord tissue mescenchymal stem cell separator |
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| Publication Number | Publication Date |
|---|---|
| CN208055308U true CN208055308U (en) | 2018-11-06 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108048298A (en) * | 2017-12-01 | 2018-05-18 | 何加铭 | A kind of umbilical cord tissue mescenchymal stem cell separator and its control method |
| CN110358673A (en) * | 2019-04-11 | 2019-10-22 | 武汉原生药谷生物医药科技有限公司 | Cell separation system and method |
-
2017
- 2017-12-01 CN CN201721648736.5U patent/CN208055308U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108048298A (en) * | 2017-12-01 | 2018-05-18 | 何加铭 | A kind of umbilical cord tissue mescenchymal stem cell separator and its control method |
| CN110358673A (en) * | 2019-04-11 | 2019-10-22 | 武汉原生药谷生物医药科技有限公司 | Cell separation system and method |
| CN110358673B (en) * | 2019-04-11 | 2022-09-13 | 武汉原生药谷生物医药科技有限公司 | Cell separation system and method |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230906 Address after: Room 1801, No. 38 Business Second Road, Baisha Road Street, Cixi City, Ningbo City, Zhejiang Province, 315302 Patentee after: Ningbo Lida pilot Biotechnology Co.,Ltd. Address before: 311225 No. 616, Building D, Jiangdong Second Road Pioneer Park, Dajiangdong Industrial Agglomeration Area, Hangzhou City, Zhejiang Province Patentee before: He Jiaming |