CN206033773U - Prokaryotes protein pretreatment systems - Google Patents
Prokaryotes protein pretreatment systems Download PDFInfo
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- CN206033773U CN206033773U CN201621080671.4U CN201621080671U CN206033773U CN 206033773 U CN206033773 U CN 206033773U CN 201621080671 U CN201621080671 U CN 201621080671U CN 206033773 U CN206033773 U CN 206033773U
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- membrane group
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- tank
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Abstract
The utility model belongs to protein preliminary treatment field, concretely relates to prokaryotes protein pretreatment systems. This system is equipped with first valve including hyperplasia jar, supersound thermostat water bath, frame filter press, micro -filtration membrane group, ultrafiltration membrane group and the centrifuge of order connection in proper order on the connecting tube between hyperplasia jar and the supersound thermostat water bath, all is equipped with the second valve on the connecting tube between supersound thermostat water bath and the frame filter press, is equipped with first booster pump on the connecting tube between frame filter press and the micro -filtration membrane group, is equipped with the second booster pump between micro -filtration membrane group and the milipore filter. The rotating cutting tool is advantaged b, including steps such as hyperplasia, ultrasonication, multi -stage filtration and centrifugations, can breed prokaryotes in a large number to acquire much more more analysis protein of treating, need not to adopt chemical reagent to carry out cell wall broken handle to prokaryotes, do not have also that the phase changes, the yield of protein is high, active good, and easily extensive batchization preliminary treatment.
Description
Technical field
This utility model belongs to protein preprocessing technical field, and in particular to a kind of prokaryote protein pretreatment system.
Background technology
Prokaryote is minimum, visually sees less than must examine under a microscope.Most prokaryotes are aquatic, its energy
Aerobic respiration is carried out under water, is the unicellular animal initially produced on the earth.The life that prokaryote is made up of prokaryotic cell
Thing, including cyanobacteria, antibacterial, actinomycetes, spirillum, mycoplasma etc..Prokaryote small volume, protein content are few, therefore to original
The biological protein of core be analyzed or processing and utilization before need to carry out corresponding pretreatment, its pretreatment should include giving birth to protokaryon
Thing carries out fast breeding, cell wall broken, Protein Extraction, protein-enriched and purification etc..
Utility model content
Technical problem to be solved in the utility model is to provide a kind of prokaryote protein pretreatment system, and which is by original
The biological propagation of core, cell wall are broken, Protein Extraction, the relevant apparatus of enrichment and purification carry out efficient combination, right to facilitate
Prokaryote protein carries out fast and effectively pretreatment.
The technical scheme that this utility model solves above-mentioned technical problem is as follows：A kind of prokaryote protein pretreatment system
System, propagation tank which includes being linked in sequence successively, ultrasonic constant temp water-bath, frame filter press, micro-filtration membrane group, ultrafilter membrane group and from
Centrifugal separator, the connecting pipe between the propagation tank and ultrasonic constant temp water-bath are provided with the first valve, the ultrasonic constant temp
The second valve is equipped with connecting pipe between water-bath and frame filter press, between the frame filter press and micro-filtration membrane group
Connecting pipe be provided with the first booster pump, be provided with the second booster pump between the micro-filtration membrane group and the ultrafilter membrane.
On the basis of above-mentioned technical proposal, this utility model can also do following improvement.
Further, the tank wall of the propagation tank is made of clear material, and the tank wall is externally provided with light compensating lamp, institute
State, the shaft of the agitator is stretched in the tank body, and the bottom of the tank body is provided with heating
Device, the tank body top are communicated with Agitation Tank, and the tank body is provided with integrated monitor device, and the integrated monitor device can monitor tank body
Interior temperature, oxygen concentration and prokaryote concentration.When prokaryote amount is less, can be using the numerous of above-mentioned propagation tank rapid, high volume
Grow prokaryote so as to which total protein reaches the degree for being available for analyzing or utilize, such as when prokaryote is cyanophyceae, can beat
Light compensating lamp reinforcement light is opened, starting heater makes culture-liquid temp in tank body maintain in the range of 35-45 DEG C, and adds by Agitation Tank
Plus appropriate Exogenous Glucose, to promote cyanophyceae fast breeding.Integrated monitor device can obtain the situation in propagation tank in real time, so as to
Appropriate regulation, which can monitor core biological concentration, can stop propagation when which reaches suitable concentration, and start follow-up pretreatment behaviour
Make.
Further, first valve and the second valve are electrically operated valve.The advantage of electrically operated valve is can be remotely electric
Dynamic control.
Further, the filter opening aperture of the filter cloth of the frame filter press is 45-55 μm.
Further, the filter opening aperture of the filter membrane of the micro-filtration membrane group is 2-5 μm.
Further, the filter opening aperture of the filter membrane of the ultrafilter membrane group is 0.25-0.45 μm.
Using above-mentioned three-stage filtration, can quickly by cell wall beyond isolating protein in the prokaryote after ultrasonication etc.
Remove completely, finally centrifugation removes Denatured protein, the purer protein that the feed liquid being centrifuged out is obtained after being pretreatment can enter
One-step freezing is concentrated to give analysis liquid or lyophilization obtains protein dry powder.
Further, the micro-filtration membrane group and ultrafiltration membrane component are not connected with back washing device.Back washing device can be periodically to microfiltration
Film group and ultrafilter membrane group carry out backwash cleaning regeneration, keep the lasting normal operation of system.
The beneficial effects of the utility model are：The pretreatment system includes propagation, ultrasonication, multistage filtering and centrifugation etc.
Step, can obtain substantial amounts of propagation prokaryote to be analyzed as needed, to obtain more protein to be analyzed, need not adopt
Cell wall break process is carried out to prokaryote with chemical reagent, also without phase-state change, effectively to rapidly remove various impurity micro-
Grain, the yield of protein are high, active good, and are easy to extensive mass pretreatment.
Description of the drawings
Fig. 1 is a kind of structural representation of prokaryote protein pretreatment system；
Fig. 2 is the structural representation of propagation tank in pretreatment system shown in Fig. 1.
In accompanying drawing, the list of parts representated by each label is as follows：
1. tank is bred；2. ultrasonic constant temp water-bath；3. frame filter press；4. micro-filtration membrane group；5. ultrafilter membrane group；6. centrifugation point
Disembark；7. the first valve；8. the second valve；9. the first booster pump；10. the second booster pump；11. light compensating lamps；12. agitators；13.
Heater；14. Agitation Tanks；15. integrated monitor devices；16. back washing devices.
Specific embodiment
Principle of the present utility model and feature are described below in conjunction with accompanying drawing, example is served only for explaining this practicality
It is new, it is not intended to limit scope of the present utility model.
As shown in Fig. 1 to 2, this utility model provides a kind of prokaryote protein pretreatment system, and which includes suitable successively
The propagation tank 1 of sequence connection, ultrasonic constant temp water-bath 2, frame filter press 3, micro-filtration membrane group 4, ultrafilter membrane group 5 and centrifugal separator 6,
Connecting pipe between the propagation tank 1 and ultrasonic constant temp water-bath 2 is provided with the first valve 7, the ultrasonic constant temp water-bath 2
Be equipped with the second valve 8 on the connecting pipe between frame filter press 3, between the frame filter press 3 and micro-filtration membrane group 4
Connecting pipe is provided with the first booster pump 9, is provided with the second booster pump 10 between the micro-filtration membrane group 4 and the ultrafilter membrane 5.
Further, the tank wall of the propagation tank 1 is made of clear material, and the tank wall is externally provided with light compensating lamp
11, agitator 12 is provided with the top of the tank body, the shaft of the agitator 12 is stretched in the tank body, the bottom of the tank body
Heater 13 is provided with, the tank body top is communicated with Agitation Tank 14, and the tank body is provided with integrated monitor device 15, the integrated prison
Survey device 15 and can monitor tank body temperature, oxygen concentration and prokaryote concentration.
Further, first valve 7 and the second valve 8 are electrically operated valve.
Further, the filter opening aperture of the filter cloth of the frame filter press 3 is 45-55 μm.
Further, the filter opening aperture of the filter membrane of the micro-filtration membrane group 4 is 2-5 μm.
Further, the filter opening aperture of the filter membrane of the ultrafilter membrane group 5 is 0.25-0.45 μm.
Further, the micro-filtration membrane group 4 and ultrafilter membrane group 5 are connected to back washing device 16.
By taking the algae protein pretreatment of cyanophyceae as an example, if the cyanophyceae amount for providing is less, can be using propagation tank faster to indigo plant
Algae carries out propagation process, and light compensating lamp is opened to high light, maintains temperature to 40-41 DEG C, adds Exogenous Glucose, breed to suitable concn
Afterwards, the first valve is opened, cyanophyceae and culture fluid are entered in ultrasonic constant temp water-bath, 30 DEG C of constant temperature, and add appropriate phosphoric acid to delay
Rush solution, ultrasonic power 800-900W, extraction time 25-30min, after the completion of open the second valve and start follow-up three-level mistake
Filter (frame, microfiltration, ultrafiltration) and related booster pump, are finally centrifuged machine removing denatured protein, in the liquid after separation
Containing purer reactive protein, analysis after can be directly used for analysis or freeze concentration or lyophilization, is used further to.
Preferred embodiment of the present utility model is the foregoing is only, it is not to limit this utility model, all in this practicality
Within new spirit and principle, any modification, equivalent substitution and improvements made etc. should be included in guarantor of the present utility model
Within the scope of shield.
Claims (7)
1. a kind of prokaryote protein pretreatment system, it is characterised in that including the propagation tank (1) being linked in sequence successively, super
Sound thermostat water bath (2), frame filter press (3), micro-filtration membrane group (4), ultrafilter membrane group (5) and centrifugal separator (6), the propagation
Connecting pipe between tank (1) and ultrasonic constant temp water-bath (2) is provided with the first valve (7), ultrasonic constant temp water-bath (2)
Be equipped with the second valve (8) on the connecting pipe between frame filter press (3), frame filter press (3) and micro-filtration membrane group
(4) connecting pipe between is provided with the first booster pump (9), is provided between micro-filtration membrane group (4) and the ultrafilter membrane (5)
Two booster pumps (10).
2. a kind of prokaryote protein pretreatment system according to claim 1, it is characterised in that the propagation tank
(1) tank wall is made of clear material, and the tank wall is externally provided with light compensating lamp (11), is provided with stirring at the top of the tank body
Device (12), the shaft of agitator (12) are stretched in the tank body, and the bottom of the tank body is provided with heater (13), described
Tank body top is communicated with Agitation Tank (14), and the tank body is provided with integrated monitor device (15), and integrated monitor device (15) can supervise
Survey tank body temperature, oxygen concentration and prokaryote concentration.
3. a kind of prokaryote protein pretreatment system according to claim 1, it is characterised in that first valve
And the second valve (8) is electrically operated valve (7).
4. a kind of prokaryote protein pretreatment system according to claim 1, it is characterised in that the frame filter pressing
The filter opening aperture of the filter cloth of machine (3) is 45-55 μm.
5. a kind of prokaryote protein pretreatment system according to claim 1, it is characterised in that the micro-filtration membrane group
(4) the filter opening aperture of filter membrane is 2-5 μm.
6. a kind of prokaryote protein pretreatment system according to claim 1, it is characterised in that the ultrafilter membrane group
(5) the filter opening aperture of filter membrane is 0.25-0.45 μm.
7. a kind of prokaryote protein pretreatment system according to any one of claim 1 to 6, it is characterised in that institute
State micro-filtration membrane group (4) and ultrafilter membrane group (5) is connected to back washing device (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621080671.4U CN206033773U (en) | 2016-09-23 | 2016-09-23 | Prokaryotes protein pretreatment systems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621080671.4U CN206033773U (en) | 2016-09-23 | 2016-09-23 | Prokaryotes protein pretreatment systems |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206033773U true CN206033773U (en) | 2017-03-22 |
Family
ID=58296824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621080671.4U Expired - Fee Related CN206033773U (en) | 2016-09-23 | 2016-09-23 | Prokaryotes protein pretreatment systems |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206033773U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107103205A (en) * | 2017-05-27 | 2017-08-29 | 湖北普罗金科技有限公司 | A kind of bioinformatics method based on proteomic image data notes eukaryotic gene group |
-
2016
- 2016-09-23 CN CN201621080671.4U patent/CN206033773U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107103205A (en) * | 2017-05-27 | 2017-08-29 | 湖北普罗金科技有限公司 | A kind of bioinformatics method based on proteomic image data notes eukaryotic gene group |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170322 Termination date: 20210923 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |