CN206219583U - Continuous sampling and state observation device in a kind of microcarrier cell digestion process - Google Patents
Continuous sampling and state observation device in a kind of microcarrier cell digestion process Download PDFInfo
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- CN206219583U CN206219583U CN201621232047.1U CN201621232047U CN206219583U CN 206219583 U CN206219583 U CN 206219583U CN 201621232047 U CN201621232047 U CN 201621232047U CN 206219583 U CN206219583 U CN 206219583U
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Abstract
The utility model provides continuous sampling and state observation device in a kind of microcarrier cell digestion process, for being sampled in digestion process and state observation to the microcarrier cell in bioreactor, including cell observation container, peristaltic pump, microscope and camera, the two ends of cell observation container pass through the outlet and entrance of the first pipeline and the second pipeline connection to the bioreactor respectively, first pipeline is extend into bioreactor under liquid level, peristaltic pump is arranged in the first pipeline, and microscope is placed between cell observation container and camera.The utility model device is simple to operate, can realize continuous sampling, and sample actual effect is good, can facilitate and treatments of the sample state is observed in time, effectively ensures that microcarrier cell can terminate digesting in the best opportunity；The device ensure that the uniformity and effective recovery sample of sample and stoste by flexibly changing the direction of rotation of peristaltic pump, it is to avoid waste.
Description
Technical field
The utility model belongs to biology techniques field, and in particular to one kind company of realization in microcarrier cell digestion process work(
Continuous sampling and the device of convenient observation Digestive States, the device ensure that sample uniformity and wave that is ageing and avoiding sample
Take.
Background technology
Micro-carriers cell culture is a kind of emerging mass cell culture technique, is the big rule of current anchorage-dependent cells
The main method of mould culture.There is homogeneous culture to have flat board culture concurrently and the advantage of the culture that suspends for it, condition of culture (temperature, pH
Value, dissolved oxygen, gas concentration lwevel etc.) it is easily controlled, and incubation systematization, automation, it is difficult to be contaminated.
The process of current micro-carriers cell culture digestion includes the culture of carrier cell, thin in the cleaning of body cell, carrier
The processes such as the digestion of born of the same parents, the digestion termination of carrier cell, existing carrier cell culture digestion operating process generally exists following
Problem：(1) representativeness of sample is poor, to grasp the optimal time point for terminating digestion, repeated sampling is just needed, after every sub-sampling
Can all there is carrier cell to remain at sample tap, so may result in the sample for taking next time inconsistent with stoste；(2) sample when
Effect property is poor, and sample is different from the environment residing for stoste from this period for being sampled to observation, more long in the external environment residence time,
I.e. there is nonsynchronous situation in sample with the Digestive States of stoste；(3) waste big, sampling number of times is more, and sample can not be reclaimed,
These samples will be discarded all, there is larger waste；(4) security is poor, and same sample tap wants being sampled for continuous several times,
Sampling interval time is short, the time of the sterilizing that do not carried out disinfection to sample tap, and pollution probability is big.
Utility model content
In order to solve the above problems, the utility model provides continuous sampling and state in a kind of microcarrier cell digestion process
Observation device.
Above-mentioned purpose of the present utility model is realized by following technical scheme：
Continuous sampling and state observation device in a kind of microcarrier cell digestion process, in bioreactor (8)
Microcarrier cell be sampled in digestion process and state observation, it is including cell observation container (2), peristaltic pump (1), micro-
Mirror (3) and camera (4), the two ends of cell observation container (2) are communicated to by the first pipeline (6) and the second pipeline (7) respectively
The outlet (81) of the bioreactor (8) and entrance (82), the first pipeline (6) extend into the liquid level in bioreactor (8)
Under, peristaltic pump (1) is arranged in the first pipeline (6), and microscope (3) is placed between cell observation container (2) and camera (4).
In above-mentioned microcarrier cell digestion process in continuous sampling and state observation device, the bioreactor (8)
Outlet (81) and entrance (82) place is respectively arranged with outlet valve (T81) and inlet valve (T82).
In above-mentioned microcarrier cell digestion process in continuous sampling and state observation device, the cell observation container (2)
It is transparent glass tube or poly- carbon ester pipe.
In above-mentioned microcarrier cell digestion process in continuous sampling and state observation device, the cell observation container (2)
It is elongated cylindrical, its a diameter of 1.0~1.5cm.
In above-mentioned microcarrier cell digestion process in continuous sampling and state observation device, the camera (4) is arranged on
On the special purpose interface of microscope (3).
In above-mentioned microcarrier cell digestion process in continuous sampling and state observation device, set on the pump housing of peristaltic pump (1)
There is the button for controlling pump head direction of rotation.
Continuous sampling and state observation device also include a controller (5) in above-mentioned microcarrier cell digestion process, described
Peristaltic pump (1), outlet valve (T81), inlet valve (T82), camera (4) are each coupled electrically to the controller (5).
Continuous sampling and state observation device also include a display screen (11) in above-mentioned microcarrier cell digestion process, described
Display screen (11) is electrically connected respectively to controller (5) and camera (4).
In above-mentioned microcarrier cell digestion process in continuous sampling and state observation device, the display screen (11) is provided with
Human-computer interaction interface.
The utility model uses above-mentioned technical proposal, obtains following technique effect：The utility model device is simple to operate, energy
Continuous sampling is enough realized, and sample actual effect is good, can facilitate and treatments of the sample state is observed in time, effectively ensures carrier cell
Can terminate digesting in the best opportunity；The device ensure that sample with stoste by the direction of rotation of flexibly change peristaltic pump
Uniformity and effective recovery sample, it is to avoid waste.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model device.
Reference is expressed as in figure：
1：Peristaltic pump, 2：Cell observation container, 3：Microscope, 4：Camera, 5：Controller, 6：First pipeline, 7：Second
Pipeline；
8：Bioreactor, 81：Outlet, 82：Entrance, T81：Outlet valve, T82：Inlet valve；
9：Motor, 10：Agitator, 11：Display.
Specific embodiment
Below in conjunction with the drawings and specific embodiments, to continuous sampling in microcarrier cell digestion process of the present utility model and
State observation device is described in detail.
Fig. 1 shows that (bold portion represents physical connection, and chain-dotted line represents electricity for the structural representation of the utility model device
Connection), as shown in figure 1, the utility model device is mainly used in the microcarrier cell in bioreactor 8 in digestion process
It is sampled and state observation, including cell observation container 2, peristaltic pump 1, microscope 3 and camera 4, cell observation container 2
Two ends are communicated to outlet 81 and the entrance 82 of bioreactor 8 by the first pipeline 6 and the second pipeline 7 respectively, and peristaltic pump 1 is set
In the first pipeline 6, microscope 3 is placed between cell observation container 2 and camera 4, wherein：
Bioreactor 8 is tank structure, outlet 81 and entrance 82 that tank body side is set, and is exporting 81 Hes respectively
Outlet valve T81, inlet valve T82 are set at entrance 82, and the outlet 81 and entrance 82 are used to connect the utility model device simultaneously
Tank interior with bioreactor 8 forms circulation loop.
Cell observation container 2 is the material of transparent glass tube, poly- carbon ester pipe or other transparent and resistance to 125 DEG C of high temperature above
The tubular container being made, it has good translucency, the container can be elongated cylindrical or other shapes, its length according to
Sampling requirement and observation condition are customized, and preferred length is 10~15cm；The diameter or width of pipe has certain limitation, too greatly
If microscope 3 be difficult to focus on, the diameter or width of pipe is preferably 1.0~1.5cm.The two ends of cell observation container 2 lead to respectively
Cross the outlet 81 of the first pipeline 6, second pipe 7 and bioreactor 8, entrance 82 to be connected, the first pipeline 6 extend into biological anti-
Under answering the liquid level in the tank body of device 8.Microscope 3 is placed directly over cell observation container 2, camera 4 is arranged on the special of microscope 3
With on interface, for the cell state in observation of cell observation container 2, and observed cell is taken pictures.
Peristaltic pump 1 is arranged on the first pipeline 6, and the power for providing sample drawn, the pump housing of the peristaltic pump is provided with
Button for controlling pump head direction of rotation, when button is pressed to the right, pump head is rotated forward along clockwise direction, when button is pressed to the left
During side, pump head is inverted counterclockwise.Peristaltic pump 1 is rotated forward for extracting sample, is inverted for making in pipeline and cell observation container 2
Sample is all back in the tank body of bioreactor 8.During sampling, outlet valve T81 and inlet valve T82 is first turned on, opened
Open peristaltic pump 1 to rotate forward, sample is circulated 20-30 seconds between bioreactor 8 and cell observation container 2, stop peristaltic pump 1
Operation, peristaltic pump 1 prevents the back flow of sample in cell observation container 2, completes primary sample.After the completion of sampling, observation, open compacted
Dynamic pump 1 is inverted, and the sample in pipeline and cell observation container 2 is all back in the tank body of bioreactor 8.
The utility model device also includes a controller 5, peristaltic pump 1, outlet valve T81, inlet valve T82, camera 4
The controller is each coupled electrically to, controller 5 is used to control the opening/stopping of each valve, the rotating forward of peristaltic pump 1, reversion and stops
Operation and take pictures action and the photo transmission order of camera 4.
The utility model device also includes a display screen 11, and the display screen is electrically connected to controller 5 and camera 4, controls
Device 5 can control camera 4 to be transferred directly to the photo of shooting to be shown on display screen 11 in real time, or will be by controller 5
It is sent to again on display screen 11；Display screen 11 is also provided with human-computer interaction interface simultaneously, for setting taking pictures for camera 4
The parameters such as frequency, every run time of sub-sampling peristaltic pump 1, and show the running status and parameter and photograph of the utility model device
The photo that camera 4 shoots, is easy to the operation and observation of experimenter.
It is assembled into microcarrier cell digestion process of the present utility model according to above-mentioned annexation with upper-part and is continuously taken
Sample and state observation device, the device are simple to operate, can realize continuous sampling, and sample actual effect is good, can facilitate to sample
Product Digestive States are observed in time, effectively ensure that carrier cell can terminate digesting in the best opportunity；The device is by flexibly changing compacted
The direction of rotation of dynamic pump 1, ensure that the uniformity and effective recovery sample of sample and stoste, it is to avoid waste.
The use step of the utility model device is as follows：
Step one：Outlet valve T81 and inlet valve T82 is opened in the control of controller 5, and is opened peristaltic pump 1 and rotated forward, and makes life
Digestive juice in thing reactor 8 together with carrier cell bioreactor 8, cell observation container 2 and between connecting pipe
Middle circulation 20~30 seconds, so that the last time sample wash in remaining in pipeline and cell observation container 2 is clean, it is ensured that take every time
The sample of sample and the uniformity of stoste；When digestive juice is full of cell observation container 2 together with carrier cell, peristaltic pump 1 is turned off, made
Sample stands 10~15 seconds in cell observation container 2.
Step 2：The focal length of regulation microscope 3 and camera 4, and camera is set by display screen 11 take pictures frequency, control
The control camera 4 of device processed 5 taken pictures to the sample in cell observation container 2, by photo upload to controller 5, and real-time exist
Shown on display screen 11.
Step 3：The photo shown by display screen 11 observe the Digestive States of sample, when having 70% on carrier~
During 80% cell detachment carrier, the carrier cell digestion being judged in bioreactor 8 is completed, and now, closes outlet valve T81
With inlet valve T82, and toward nutrient solution is added in bioreactor 8, terminate digestion；Otherwise, return to step one, repeats above-mentioned mistake
Journey.
It will be appreciated by those skilled in the art that these embodiments are merely to illustrate the utility model without limiting this practicality newly
The scope of type, the various equivalent variations made to the utility model and modification belong to the utility model disclosure.
Claims (9)
1. continuous sampling and state observation device in a kind of microcarrier cell digestion process, in bioreactor (8)
Microcarrier cell is sampled and state observation in digestion process, it is characterised in that including cell observation container (2), wriggle
Pump (1), microscope (3) and camera (4), the two ends of cell observation container (2) pass through the first pipeline (6) and the second pipeline respectively
(7) outlet (81) and the entrance (82) of the bioreactor (8) are communicated to, the first pipeline (6) extend into bioreactor (8)
Under interior liquid level, peristaltic pump (1) is arranged in the first pipeline (6), and microscope (3) is placed in cell observation container (2) and camera
(4) between.
2. continuous sampling and state observation device in microcarrier cell digestion process according to claim 1, its feature exist
In the outlet (81) of the bioreactor (8) and entrance (82) place are respectively arranged with outlet valve (T81) and inlet valve
(T82)。
3. continuous sampling and state observation device in microcarrier cell digestion process according to claim 1, its feature exist
In the cell observation container (2) is transparent glass tube or poly- carbon ester pipe.
4. continuous sampling and state observation device in microcarrier cell digestion process according to claim 3, its feature exist
In the cell observation container (2) is elongated cylindrical, its a diameter of 1.0~1.5cm.
5. continuous sampling and state observation device in microcarrier cell digestion process according to claim 1, its feature exist
In the camera (4) is on the special purpose interface of microscope (3).
6. continuous sampling and state observation device in microcarrier cell digestion process according to claim 1, its feature exist
In the pump housing of peristaltic pump (1) is provided with the button for controlling pump head direction of rotation.
7. continuous sampling and state observation device in the microcarrier cell digestion process according to any one of claim 1 to 6,
Characterized in that, also including a controller (5), the peristaltic pump (1), outlet valve (T81), inlet valve (T82), camera
(4) it is each coupled electrically to the controller (5).
8. continuous sampling and state observation device in microcarrier cell digestion process according to claim 7, its feature exist
In also including a display screen (11), the display screen (11) is electrically connected respectively to controller (5) and camera (4).
9. continuous sampling and state observation device in microcarrier cell digestion process according to claim 8, its feature exist
In the display screen (11) is provided with human-computer interaction interface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201621232047.1U CN206219583U (en) | 2016-11-17 | 2016-11-17 | Continuous sampling and state observation device in a kind of microcarrier cell digestion process |
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| CN201621232047.1U CN206219583U (en) | 2016-11-17 | 2016-11-17 | Continuous sampling and state observation device in a kind of microcarrier cell digestion process |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441424A (en) * | 2018-04-23 | 2018-08-24 | 福州大北农生物技术有限公司 | A kind of microcarrier biological reactor and its culture porcine circovirus 2 type method |
| CN108441413A (en) * | 2018-03-28 | 2018-08-24 | 安徽东方帝维生物制品股份有限公司 | A kind of microcarrier suspension culture on-line period device and operating method |
| CN108562459A (en) * | 2018-03-20 | 2018-09-21 | 骆中庚 | A kind of sampler of bioreactor |
-
2016
- 2016-11-17 CN CN201621232047.1U patent/CN206219583U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108562459A (en) * | 2018-03-20 | 2018-09-21 | 骆中庚 | A kind of sampler of bioreactor |
| CN108562459B (en) * | 2018-03-20 | 2020-12-04 | 阮炳旭 | Sampling device of bioreactor |
| CN108441413A (en) * | 2018-03-28 | 2018-08-24 | 安徽东方帝维生物制品股份有限公司 | A kind of microcarrier suspension culture on-line period device and operating method |
| CN108441413B (en) * | 2018-03-28 | 2020-05-26 | 安徽东方帝维生物制品股份有限公司 | Microcarrier suspension culture online sampling device and operation method |
| CN108441424A (en) * | 2018-04-23 | 2018-08-24 | 福州大北农生物技术有限公司 | A kind of microcarrier biological reactor and its culture porcine circovirus 2 type method |
| CN108441424B (en) * | 2018-04-23 | 2023-08-22 | 福州大北农生物技术有限公司 | Microcarrier biological reaction tank and method for culturing porcine circovirus type 2 by same |
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