CN201296758Y - Rotary filter for bioreactor and bioreactor comprising same - Google Patents
Rotary filter for bioreactor and bioreactor comprising same Download PDFInfo
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- CN201296758Y CN201296758Y CN 200820122457 CN200820122457U CN201296758Y CN 201296758 Y CN201296758 Y CN 201296758Y CN 200820122457 CN200820122457 CN 200820122457 CN 200820122457 U CN200820122457 U CN 200820122457U CN 201296758 Y CN201296758 Y CN 201296758Y
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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Abstract
The utility model relates to a rotary filter (1) for a bioreactor, which is characterized by comprising a shell (13), a hollow rotating shaft (14), a collection chamber (16) and a motor (18), wherein the motor (18) drives the hollow rotating shaft (14) and the shell (13) to rotate; one end of the hollow rotating shaft (14) is communicated with a chamber enclosed by the shell (13); the hollow rotating shaft (14) are integrally formed or in sealing connection with the shell (13); the other end of the hollow rotating shaft (14) is communicated with the collection chamber (16); and the shell (13) is provided with a filter screen or made of the filter screen. The rotary filter for the bioreactor is arranged outside a culture tank, the mounting and the replacement are convenient, and on-line cleaning can be performed without controlling the liquid level of a culture medium in the culture tank and restricting the stirring mode of the culture tank.
Description
Technical field
The utility model relates to a kind of revolving filter and comprises the bio-reactor of this strainer, is specifically related to a kind of bio-reactor that is used for the revolving filter of bio-reactor and comprises this revolving filter.
Background technology
In the liquid culture process of isolated organ (for example tip of a root of plant, stem apex, cotyledon, leaf primordium, the flower primordium etc.) in vitro tissue (for example callus of plant) of organism of organism (for example various microorganisms of bacterium, fungi, virus etc.), organism or the cell (for example various zooblast) of organism, all need to regularly replace substratum guaranteeing supply, and in time discharge the metabolic waste of cultured object to the enough nutrition of cultured object.Also need sampling at any time from the substratum at cultured object place simultaneously, with the growing state of monitoring cultured object.Therefore to carry out that biological liquid cultivates device---the requirement of bio-reactor is very high, such as in carrying out described replacing substratum and sampling testing process, strictness prevents that assorted bacterium from entering into the pollution that bio-reactor causes cultured object from various channels.
As shown in Figure 1, the prior biological reactor comprises culture tank 102, feed storage tank 103, collection storage tank 104 and the revolving filter 101 that is positioned at culture tank 102, and wherein, revolving filter 101 is fixed on the solid stir shaft 109 of culture tank 102.The rotation of solid stir shaft 109 drives paddle 110 and revolving filter 101 fixed thereon and rotates together, prevents the cultured object in the substratum or the filtering net of its clogged with fragments revolving filter.Culture tank 102 links to each other by feed-pipe 105 with feed storage tank 103; Culture tank 102 links to each other by collection tube 107 with collection storage tank 104.Feed-pipe 105 1 end openings stretch into feed storage tank 103 bottoms from feed storage tank 103 tops, and the other end opening is positioned at culture tank 102 tops, and fresh feed pump 106 is housed on the feed-pipe 105, fresh culture can be pumped into culture tank 102.Collection tube 107 1 end openings stretch into revolving filter 101 bottoms through culture tank 102 top covers from revolving filter 101 tops, the other end opening is positioned at collects storage tank 104 tops, and collecting pump 108 is housed on the collection tube 107, can pumps into and collect storage tank 104 filtering out substratum behind cultured object or its fragment.Substratum in the collection tube can be used for (a) and detect the cultured object metabolite content with monitoring cultured object growing state; (b) separate the useful products (such as penicillin, antibody etc.) that produces by cultured object metabolism; (c) reclaiming wherein nutritive ingredient by treatment steps such as purifying treatment utilizes etc. again.
The existing revolving filter shortcoming that is used for bio-reactor comprises: revolving filter upper end open, the liquid level of nutrient solution in the restriction culture tank.
As shown in Figure 1, the bio-reactor revolving filter of prior art, lower end closed, upper end open is to guarantee that collection tube can be inserted into filter bottom and also needn't rotate with the rotation of revolving filter.Therefore the liquid level of nutrient solution can not surpass the opening of revolving filter upper end in the culture tank, otherwise the nutrient solution that has cultured object or its fragment in the culture tank will sneak in the revolving filter, does not reach the purpose that obtains filtrate.Therefore the bio-reactor of prior art must comprise the device of the substratum liquid level height in the accurate control bio-reactor, such as level electrode etc., has increased the cost of bio-reactor greatly.
As shown in Figure 1, use the shortcoming of the bio-reactor of existing revolving filter to comprise:
(1) revolving filter installing/dismounting complicated operation.
Bio-reactor usually is used for the liquid culture of large-scale organism or its isolated organ, tissue and cell.According to demand of practical production, the scale of bio-reactor can be raised to several kilolitres from several, even reaches tons up to ten thousand, millions of tons (such as the required fermentor tank of production of antibiotics).Because behind the bio-reactor operation certain hour, cultured object or its fragment may stop up the filtering net of revolving filter, must dismantle revolving filter and clean its filtering net.The prior biological reactor must be installed revolving filter earlier when installing first, the culture tank top cover is installed again; When the dismounting revolving filter, must at first open top cover.If but in cultured object growth cycle process, in a single day the top cover of bio-reactor is opened, cultured object wherein contacts with extraneous, can cause the generation of pollution incident.
(2) can't on-line cleaning
The advantage of bio-reactor is and can cultivates organism and isolated organ, tissue and cell by continuous large-scale, but every kind of cultured object all has its certain growth cycle.If rotate the obstruction of strainer in the cultured object growth cycle, the prior biological reactor can't be realized not stopping the cultivation in the bio-reactor and be rotated the cleaning of strainer.In addition,, can stop cultivating, also must revolving filter be disassembled, carry out ultrasonic cleaning according to above-mentioned complex operations even a growth cycle finishes.
(3) the stir shaft load is big, increase sealing difficulty, and its motor takies the culture tank superjacent air space
Because the revolving filter of prior art is driven by the solid stir shaft of culture tank, thereby stir shaft not only will drive paddle and rotate, but also the rotation of the revolving filter of will loading, thereby need powerful motor that power is provided.And the power of motor is big more, its volume that takies is also big more, thereby the motor of prior art bio-reactor stir shaft can take the finite space of bioreactor culture jar top, requirement to the bio-reactor auxiliary facility is improved, increase such as the requirement to the height of equipment place factory building, the maintenance difficulty increases.
In addition, because the load of stir shaft increases, the mechanical seal difficulty of stir shaft and culture tank top cover also must increase, and causes the bio-reactor sealing easily not exclusively, causes the generation of pollution incident.
To sum up, prior art is used for the revolving filter of bio-reactor and comprises that also there is above shortcoming in the bio-reactor of revolving filter, needs a kind of bio-reactor that can overcome the revolving filter that is used for bio-reactor of above-mentioned shortcoming and comprise this revolving filter badly.
The utility model content
Technical problem to be solved in the utility model is to overcome the revolving filter that is used for bio-reactor that is installed in culture tank inside at present must accurately control substratum liquid level in the culture tank, the installing/dismounting complicated operation, can't on-line cleaning, drive by the culture tank stir shaft and to cause that the culture tank sealing difficulty is big, motor takes up room big shortcoming; A kind of revolving filter that is used for bio-reactor that is installed in the culture tank outside is provided, and it is convenient install to change, can on-line cleaning, need not to control substratum in the culture tank liquid level, do not limit the alr mode of culture tank.
In order to solve the problems of the technologies described above, the technical scheme that the utility model adopted is: a kind of revolving filter 1 that is used for bio-reactor is provided, wherein: this revolving filter 1 comprises housing 13, hollow rotary shaft 14, collection chamber 16 and motor 18, wherein, motor 18 drives hollow rotary shaft 14 and housing 13 rotations; One end of hollow rotary shaft 14 communicates with the chamber that housing 13 surrounds, and hollow rotary shaft 14 and housing 13 are integrally formed or be tightly connected; The other end of hollow rotary shaft 14 communicates with collection chamber 16; Described housing 13 is provided with filtering net or is made by filtering net.
The utility model also provides a kind of bio-reactor that uses the utility model revolving filter, and wherein: this bio-reactor comprises culture tank 2, sleeve 12 and revolving filter 1, and wherein culture tank 2 and sleeve 12 communicate, and sleeve 12 is positioned at culture tank 2 outsides; Revolving filter 1 is positioned at sleeve; This revolving filter 1 comprises housing 13, hollow rotary shaft 14, collection chamber 16 and motor 18, and wherein, motor 18 drives hollow rotary shaft 14 and housing 13 rotates; One end of hollow rotary shaft 14 communicates with the chamber that housing 13 surrounds, and hollow rotary shaft 14 and housing 13 are integrally formed or be tightly connected; The other end of hollow rotary shaft 14 communicates with collection chamber 16; Described housing 13 is provided with filtering net or is made by filtering net.
Compared with prior art the beneficial effect that the utility model had is: because the housing 13 of revolving filter of the present utility model is provided with filtering net or is made by filtering net, and there is not direct opening between the culture tank as prior art, therefore nutrient solution can filtered just flow directly into and pollutes filtrate in the culture tank, thereby need not to limit the liquid level of nutrient solution in the culture tank.Need not to install the also parts of the interior nutrient solution page of culture tank of accurate control thus, thereby greatly reduce cost.
Bio-reactor of the present utility model because sleeve 12 is positioned at culture tank 2 outsides and revolving filter 1 is positioned at sleeve, therefore can be implemented in line and cleans.Promptly need not to stop the interior culturing process of culture tank, just can carry out at any time dismounting, replacing and ultrasonic cleaning the revolving filter that in the cultured object growth cycle, takes place to stop up to cultured object.Under the situation that can stop cultivating, more can dismantle at an easy rate, replacing and ultrasonic cleaning the revolving filter that take place to stop up.Owing to need not to use the solid turning axle in the culture tank to drive revolving filter of the present utility model, therefore reduced the load of culture tank stir shaft greatly, reduced the difficulty of mechanical seal, and its motor takies the culture tank superjacent air space and significantly reduces.And those skilled in the art can also adopt other alr mode as required, and such as only in the culture tank bottom rotatable paddle being set, bio-reactor maintenance therefore of the present utility model is more convenient.
Description of drawings
Fig. 1 is built-in revolving filter of prior art and the bioreactor construction synoptic diagram that comprises this revolving filter;
Fig. 2 is the utility model embodiment 1 external placed type revolving filter and the bioreactor construction synoptic diagram that comprises this revolving filter;
Fig. 3 is the utility model embodiment 2 external placed type revolving filters and the bioreactor construction synoptic diagram that comprises this revolving filter.
Among Fig. 1:
101 revolving filters, 102 culture tank, 103 feed storage tank 104 are collected storage tank
105 feed-pipes, 106 fresh feed pumps, 107 collection tubes, 108 collecting pumps
109 solid stir shaft 110 paddles
Among Fig. 2 and Fig. 3:
1 revolving filter, 2 culture tank, 3 feed storage tank
4 collect storage tank 5 feed-pipes 6 fresh feed pumps
7 collection tubes, 8 collecting pumps, 9 discharge nozzles
10 discharging pumps, 11 feed back pipes, 12 sleeves
13 revolving filter housings, 14 hollow rotary shafts, 15 hollow rotary shaft chambers
16 collection chamber, 17 mechanical seals, 18 motors
Embodiment
The revolving filter 1 that is used for bio-reactor of the present utility model, wherein, this revolving filter 1 comprises housing 13, hollow rotary shaft 14, collection chamber 16 and motor 18, wherein, motor 18 drives hollow rotary shaft 14 and housing 13 rotates; One end of hollow rotary shaft 14 communicates with the chamber that housing 13 surrounds, and hollow rotary shaft 14 and housing 13 are integrally formed or be tightly connected; The other end of hollow rotary shaft 14 communicates with collection chamber 16; Described housing 13 is provided with filtering net or is made by filtering net.
Wherein, motor 18 can be this area various motors that revolving filter is rotated commonly used, such as fdbm, dc servomotor, inverter motor, step motor, gear motor and linear motor etc.The turning unit (such as hollow rotary shaft 14) that motor drives adopts mechanical seal 17 (delimiting with circle) with the interface (such as collection chamber 16 and sleeve 12) of fixing part in Fig. 2 and Fig. 3,, because making the filtrate in the collection chamber 16 contact with outside air, the rotation of hollow rotary shaft perhaps prevents to prevent because the rotation of hollow rotary shaft mixes the filtrate in sleeve 12 interior unfiltered nutrient solutions and the collection chamber 16.
Described mechanical seal (mechanical seal) is meant the device that prevents escape of liquid that is made of keeping applying and relatively sliding under the effect of hydrodynamicpressure and shim mechanism elastic force (or magnetic force) and under the cooperation of auxiliary seal perpendicular to the end face of rotation at least one pair of.Such as the wavy metal seal of tube is that elastic force load maintainer and auxiliary seal are the mechanical seal of metallicity line pipe.The composition of mechanical seal generally comprises: a. major parts: rotating ring and stationary ring.B. auxiliary sealing member: sealing-ring (O shape, X-shaped, U type, wedge shape, rectangle soft graphite, the rubber-coated O circle of PTFE etc. are arranged).C. stretch compensation mechanism: spring, throw-out collar.D. driving member: play dustpan seat and key or various screw.
Described hollow rotary shaft 14 and revolving filter housing 13 can be one of the forming, and help the two synchronous rotation.Described hollow rotary shaft 14 and revolving filter housing 13 also can be two parts that are tightly connected, and help dismounting and clean respectively.Especially probability that stops up and the influence of exchanging liquid process and sampling process take place usually greater than hollow rotary shaft 14 in housing 13, need to clean separately revolving filter housing 13.Therefore preferred described hollow rotary shaft 14 and being tightly connected of revolving filter housing 13 are shrink-fit, can prevent the interface pollution filtrate of the nutrient solution of filtered in the sleeve 12 by hollow rotary shaft 14 and revolving filter housing 13.Preferred described housing 13 tops and bottom are the stainless steel substrates sealing, and middle portion is provided with filtering net.
The filtering net of described housing 13 can be operable various filtering nets in the biological culture, such as stainless steel filtering net, peptide alloy filtering net, nylon filtering net.Those skilled in the art can select the number of plies of filtering net according to the diameter of cultured object and fragment thereof, such as the 1-6 layer.And the utility model does not have special requirement to the mesh diameter of filtering net, as long as the filtering net mesh diameter of described housing 13 is less than the diameter of cultured object in the bio-reactor or its fragment.According to the size of cultured object, those skilled in the art can select mesh, are 5-150um such as the diameter of preferred filtering net mesh.
Described cultured object is selected from the group of being made up of the cell of in vitro tissue of the isolated organ of organism, organism, organism and organism.Described organism can for this area can practical liquid culture microorganism, such as virus, viroid, soft virus, bacterium, actinomycetes, chlamydozoan, mycoplasma, Rickettsiae, cyanobacteria, fungi (as yeast), micro-algae, protozoon etc.The isolated organ of described organism can be the tip of a root of plant, stem apex, cotyledon, leaf primordium, flower primordium etc.The in vitro tissue of described organism can be callus of plant etc.The three-dimensional cell of described organism can for the cells of various organisms such as zooblast.Described cultured object can also be cultivated attached to obtaining in liquid on the microcarrier such as zooblast attached on certain carrier.Described zooblast can be passage cell and diploid cells such as MRC5,2BS such as BHK21 cell, Chinese hamster ovary celI, NS0 cell, Vero cell, 293 cells, Mac145 cell, MA104 cell, PK cell, ST cell, mdck cell; Primary cells such as bull testis, suslik kidney.The size of the cell of the isolated organ of described organism, organism, the in vitro tissue of organism and organism is as well known to those skilled in the art.
Preferably, a end that can described hollow rotary shaft 14 inserts the chamber that housing 13 surrounds, and the internal diameter of hollow rotary shaft 13 openings more helps the collection of filtrate less than the shell internal diameter of body 14 of rotating filter.The other end of described hollow rotary shaft 14 is that hollow rotary shaft 14 parts are positioned at collection chamber 16 with collection chamber 16 modes of communicating, and hollow rotary shaft 14 is positioned at hole (as shown in Fig. 2 and Fig. 3, the hollow axle axle that revolves has opening on sidewall) or filtering net is set on the part of collection chamber 16.Filtering net preferably is set, can carries out secondary filtration filtrate.The filtering net of described hollow rotary shaft 14 satisfies the requirement of above-mentioned housing 13 filtering nets, and the filtering net of preferred described hollow rotary shaft 14 is littler than the mesh diameter of above-mentioned housing 13 filtering nets.
Preferred described collection chamber 16 communicates with collection storage tank 4 by collection tube 7, and collection tube 7 is provided with the nutrient solution after filtering is pumped into the collecting pump 8 of collecting storage tank 4 by collection chamber 16, flows to collection chamber 16 and collects storage tank 4 through hollow rotary shaft chamber 15 from the chamber that housing 13 surrounds more to help filtrate by the generation negative pressure.
Bio-reactor of the present utility model is for using the bio-reactor of the utility model revolving filter, and wherein, this bio-reactor comprises culture tank 2, sleeve 12 and revolving filter 1, and wherein culture tank 2 and sleeve 12 communicate, and sleeve 12 is positioned at culture tank 2 outsides; Revolving filter 1 is positioned at sleeve; This revolving filter 1 comprises housing 13, hollow rotary shaft 14, collection chamber 16 and motor 18, and wherein, motor 18 drives hollow rotary shaft 14 and housing 13 rotates; One end of hollow rotary shaft 14 communicates with the chamber that housing 13 surrounds, and hollow rotary shaft 14 and housing 13 are integrally formed or be tightly connected; The other end of hollow rotary shaft 14 communicates with collection chamber 16; Described housing 13 is provided with filtering net or is made by filtering net.
Bio-reactor described in the utility model can wherein, use revolving filter of the present utility model for mechanical agitation type bio-reactor, magnetic agitation formula bio-reactor, air lift type biotinylated biomolecule reactor or wave bio-reactor.
In the bio-reactor of the present utility model, culture tank 2 and sleeve 12 communicate, and sleeve 12 is positioned at culture tank 2 outsides; Revolving filter 1 is positioned at sleeve.Such structure can be implemented in line and cleans.Promptly need not to stop the interior culturing process of culture tank, just can carry out at any time dismounting, replacing and ultrasonic cleaning the revolving filter that in the cultured object growth cycle, takes place to stop up to cultured object.Under the situation that can stop cultivating, more can dismantle at an easy rate, replacing and ultrasonic cleaning the revolving filter that take place to stop up.Owing to need not to use the solid turning axle in the culture tank to drive revolving filter of the present utility model, therefore reduced the load of culture tank stir shaft greatly, reduced the difficulty of mechanical seal, and its motor takies the culture tank superjacent air space and significantly reduces.And those skilled in the art can also adopt other alr mode as required, and such as only in the culture tank bottom rotatable paddle being set, bio-reactor maintenance therefore of the present utility model is more convenient.
Wherein, being tightly connected of described hollow rotary shaft 14 and housing 13 is shrink-fit.The filtering net mesh diameter of described housing 13 is less than the diameter of cultured object in the bio-reactor or its fragment.Described cultured object is selected from the group of being made up of the cell of in vitro tissue of the isolated organ of organism, organism, organism and organism.The filtering net mesh diameter of described housing (13) is 5-150um.One end of described hollow rotary shaft 14 inserts the chamber that housing 13 surrounds.The other end of described hollow rotary shaft 14 is that hollow rotary shaft 14 parts are positioned at collection chamber 16 with collection chamber 16 modes of communicating, and hollow rotary shaft 14 is positioned on the part of collection chamber 16 hole or filtering net are set.Described collection chamber 16 communicates with collecting storage tank 4 by collection tube 7, and collection tube 7 is provided with the collecting pump 8 that nutrient solution is pumped into collection storage tank 4 by collection chamber 16.In addition, under the situation that revolving filter stops up, can also liquid (as filtrate or fresh culture or washing lotion) be pumped into collection chamber 16 by collecting storage tank 4 by oppositely using collecting pump 8 pressurizations; Liquid pours in revolving filter 1 through collection chamber 16, hollow rotary shaft chamber 15, oppositely by the filtering net on the housing 13 of revolving filter, plays the effect of stopping up of eliminating.
Preferred described culture tank 2 and sleeve 12 communicate by discharge nozzle 9 and feed back pipe 11; Wherein discharge nozzle 9 one end openings stretch into culture tank 2 bottoms from culture tank 2 tops, and the other end opening is positioned at sleeve 12 bottoms, and discharge nozzle 9 is provided with the discharging pump 10 that nutrient solution is pumped into sleeve 12 by culture tank 2.Described feed back pipe 11 is provided with the feed back pump that nutrient solution is pumped into culture tank 2 by sleeve 12.Such structure can guarantee that the smooth of liquid flows between culture tank 2 and the sleeve 12.
In addition, bio-reactor of the present utility model as shown in Figure 3 can also comprise feed storage tank 3.Culture tank 2 links to each other by feed-pipe 5 with feed storage tank 3.Feed-pipe 5 one end openings stretch into feed storage tank 3 bottoms from feed storage tank 3 tops, and the other end opening is positioned at culture tank 2 tops, and fresh feed pump 6 is housed on the feed-pipe 5, fresh culture can be pumped into culture tank 2.
Below in conjunction with Fig. 2 and Fig. 3, specify the working process that the utility model is used for the revolving filter of bio-reactor and comprises the bio-reactor of this revolving filter.
Embodiment 1
Present embodiment is used for explanation: revolving filter of the present utility model and the bio-reactor that comprises this revolving filter are used to take a sample at any time with the working process of the growing state of monitoring cultured object.
As shown in Figure 2, comprise the nutrient solution of cultured object in the culture tank 2, under the effect of discharging pump 10, enter sleeve 12 through discharge nozzle 9.The housing 13 that motor 18 drives hollow rotary shaft 14 and revolving filter rotates.The sleeve 12 internal filters 1 outer filtering net of substratum on revolving filter housing 13 forms filtrate and enters the chamber that housing surrounds, and cultured object and fragment thereof are stayed outside the revolving filter housing 13, and because the rotation of revolving filter housing 13, cultured object is easy to be dispersed in sleeve 12 internal filters 1 chamber outward.This sleeve 12 internal filters 1 outer nutrient solution can be got back to culture tank 2 through feed back pipe 11.
Filtrate in the chamber that housing 13 surrounds via hollow rotary shaft chamber 15, is positioned at the hole that is provided with on the part of collection chamber 16 by hollow rotary shaft 14, enters collection chamber 16.Described collection chamber 16 communicates with collecting storage tank 4 by collection tube 7, and collection tube 7 is provided with the collecting pump 8 that filtered liquid is pumped into collection storage tank 4 by collection chamber 16.By the negative pressure that collecting pump 8 produces, filtrate is flowed into from collection chamber 16 collect storage tank 4.
Collect the filtrate of collecting in the storage tank 4, can be used for the various indexs that the detection reaction cultured object is cultivated situation, also can be used to separate the purification useful metabolite.
Present embodiment is used for explanation: revolving filter of the present utility model and the bio-reactor that comprises this revolving filter are used to take a sample at any time and/or change the working process of substratum.
As shown in Figure 3, culture tank 2 links to each other by feed-pipe 5 with feed storage tank 3.Fresh culture in the feed storage tank 3 under the effect of fresh feed pump 6, enters culture tank 2 through feed-pipe 5.
The nutrient solution that comprises cultured object in the culture tank 2 under the effect of discharging pump 10, enters sleeve 12 through discharge nozzle 9.The housing 13 that motor 18 drives hollow rotary shaft 14 and revolving filter rotates.The sleeve 12 internal filters 1 outer filtering net of substratum on revolving filter housing 13 forms filtrate and enters the chamber that housing surrounds, and cultured object and fragment thereof are stayed outside the revolving filter housing 13, and because the rotation of revolving filter housing 13, cultured object is easy to be dispersed in sleeve 12 internal filters 1 chamber outward.This sleeve 12 internal filters 1 outer nutrient solution can be got back to culture tank 2 through feed back pipe 11.
Filtrate in the chamber that housing 13 surrounds via hollow rotary shaft chamber 15, is positioned at the hole that is provided with on the part of collection chamber 16 by hollow rotary shaft 14, enters collection chamber 16.Described collection chamber 16 communicates with collecting storage tank 4 by collection tube 7, and collection tube 7 is provided with the collecting pump 8 that filtered liquid is pumped into collection storage tank 4 by collection chamber 16.By the negative pressure that collecting pump 8 produces, filtrate is flowed into from collection chamber 16 collect storage tank 4.
Fresh culture constantly flows into culture tank 2 in the feed storage tank 3, the chamber that the chamber that outmoded substratum surrounds through discharge nozzle 9, sleeve 12, revolving filter housing 13 surround forms filtrate, and filtrate arrives through hollow rotary shaft chamber 15, collection chamber 16, collection tube 7 and collects storage tank 4.Constantly increase along with collecting the filtrate of collecting in the storage tank 4, can realize the continuous replacing of substratum in the culture tank 2.Gained filtrate can be used for the various indexs that the detection reaction cultured object is cultivated situation, also can be used to separate purify have utilize be worth meta-bolites.
Present embodiment provides revolving filter of the present utility model to be used for the example of 100L bio-reactor suspension culture BHK21 cell.
Cultured object: BHK21C13 cell
Culture tank: 100L (CLavorus THR 100L series zooblast reactor)
Cell culture medium: BHSM substratum (Beijing Qingdatianyi Bioisystech Co., Ltd)
Cell cultivation process:
As shown in Figure 3, the cell inoculation density of culture tank is 1 * 10 in the bio-reactor
5Cell/ml, the temperature of control culture tank 2 is that 36-37 ℃, pH are that 6.8-7.3, dissolved oxygen are 30-60%, carries out the BHK21 suspension culture of cells, cell grows to about 1.5-3 * 10
6During cell/ml, begin to change nutrient solution by revolving filter 1 of the present utility model, the rotating speed of revolving filter 1 is 60-180rpm, and the feeding rate of feed storage tank 3 and the collection rate of collecting storage tank 4 promptly are 0.3-2 culture tank volume/sky.
The result:
The stand density of the BHK21 cell cultured continuously in the 100L zooblast reactor all can reach 10
7More than the cells/ml, move 10 days continuously, revolving filter of the present utility model stops up.
Present embodiment provides revolving filter of the present utility model to be used for the example of 100L bio-reactor microcarrier suspension culture Vero cell.
Cultured object: Vero cell
Culture tank: 100L (CLavorus THR 100L series zooblast reactor)
Cell culture medium: VHM substratum (Beijing Qingdatianyi Bioisystech Co., Ltd)
Cell cultivation process:
As shown in Figure 3, the microcarrier concentration of culture tank is 5g/L in the bio-reactor, and Vero cell inoculation density is 2-5 * 10
5Cell/ml, the temperature of control culture tank 2 is that 36-37 ℃, pH are that 6.8-7.3, dissolved oxygen are 30-60%, carry out the microcarrier suspension culture of Vero cell, the cell growth is after 2 days, begin to change nutrient solution by revolving filter 1 of the present utility model, the rotating speed of revolving filter 1 is 60-180rpm, and the collection rate of the feeding rate of feed storage tank 3 and collection storage tank 4 promptly is 0.3-2 culture tank volume/sky.
The result:
The stand density of the Vero cell cultured continuously in the 100L zooblast reactor all can reach 5 * 10
6Level is moved 20 days continuously, and revolving filter of the present utility model stops up.
Use revolving filter of the present utility model and comprise the bio-reactor of this revolving filter, it is little that the cultivation organism and the part that exsomatizes thereof are polluted probability, can online at any time sampling, online installing/dismounting revolving filter stops up with cleaning or clean revolving filter, cost is low, revolving filter and comprise that the maintenance of bio-reactor of this revolving filter is more convenient.
Claims (22)
1, a kind of revolving filter (1) that is used for bio-reactor, it is characterized in that: this revolving filter (1) comprises housing (13), hollow rotary shaft (14), collection chamber (16) and motor (18), wherein, motor (18) drives hollow rotary shaft (14) and housing (13) rotation; One end of hollow rotary shaft (14) communicates with the chamber that housing (13) surrounds, and hollow rotary shaft (14) and housing (13) are integrally formed or be tightly connected; The other end of hollow rotary shaft (14) communicates with collection chamber (16); Described housing (13) is provided with filtering net or is made by filtering net.
2, revolving filter according to claim 1 is characterized in that: being tightly connected of described hollow rotary shaft (14) and housing (13) is shrink-fit.
3, revolving filter according to claim 1 is characterized in that: the filtering net mesh diameter of described housing (13) is less than the diameter of cultured object in the bio-reactor or its fragment.
4, revolving filter according to claim 3 is characterized in that: described cultured object is selected from the group of being made up of the cell of in vitro tissue of the isolated organ of organism, organism, organism and organism.
5, revolving filter according to claim 4 is characterized in that, described cultured object is a zooblast.
6, revolving filter according to claim 5, it is characterized in that described cultured object is selected from the group of being made up of BHK21 cell, Chinese hamster ovary celI, NSO cell, Vero cell, 293 cells, Mac145 cell, MA104 cell, PK cell, ST cell, mdck cell, MRC5 cell, 2BS cell, bull testis cell, suslik kidney primary cell.
7, revolving filter according to claim 3 is characterized in that: the filtering net mesh diameter of described housing (13) is 5-150um.
8, revolving filter according to claim 1 is characterized in that: an end of described hollow rotary shaft (14) inserts the chamber that housing (13) surrounds.
9, revolving filter according to claim 1, it is characterized in that: the other end of described hollow rotary shaft (14) is that hollow rotary shaft (14) part is positioned at collection chamber (16) with collection chamber (16) mode of communicating, and hollow rotary shaft (14) is positioned on the part of collection chamber (16) hole or filtering net are set.
10, revolving filter according to claim 1, it is characterized in that: described collection chamber (16) communicates with collecting storage tank (14) by collection tube (7), and collection tube (7) is provided with the collecting pump (8) that nutrient solution is pumped into collection storage tank (14) by collection chamber (16).
11, a kind of bio-reactor is characterized in that: this bio-reactor comprises culture tank (2), sleeve (12) and revolving filter (1), and wherein culture tank (2) and sleeve (12) communicate, and sleeve (12) is positioned at culture tank (2) outside; Revolving filter (1) is positioned at sleeve; This revolving filter (1) comprises housing (13), hollow rotary shaft (14), collection chamber (16) and motor (18), and wherein, motor (18) drives hollow rotary shaft (14) and housing (13) rotates; One end of hollow rotary shaft (14) communicates with the chamber that housing (13) surrounds, and hollow rotary shaft (14) and housing (13) are integrally formed or be tightly connected; The other end of hollow rotary shaft (14) communicates with collection chamber (16); Described housing (13) is provided with filtering net or is made by filtering net.
12, bio-reactor according to claim 11 is characterized in that: being tightly connected of described hollow rotary shaft (14) and housing (13) is shrink-fit.
13, bio-reactor according to claim 11 is characterized in that: the filtering net mesh diameter of described housing (13) is less than the diameter of cultured object in the bio-reactor or its fragment.
14, bio-reactor according to claim 13 is characterized in that: described cultured object is selected from the group of being made up of the cell of in vitro tissue of the isolated organ of organism, organism, organism and organism.
15, bio-reactor according to claim 14 is characterized in that, described cultured object is a zooblast.
16, bio-reactor according to claim 15, it is characterized in that described cultured object is selected from the group of being made up of BHK21 cell, Chinese hamster ovary celI, NSO cell, Vero cell, 293 cells, Mac145 cell, MA104 cell, PK cell, ST cell, mdck cell, MRC5 cell, 2BS cell, bull testis cell, suslik kidney primary cell.
17, bio-reactor according to claim 13 is characterized in that: the filtering net mesh diameter of described housing (13) is 5-150um.
18, bio-reactor according to claim 11 is characterized in that: an end of described hollow rotary shaft (14) inserts the chamber that housing (13) surrounds.
19, bio-reactor according to claim 11, it is characterized in that: the other end of described hollow rotary shaft (14) is that hollow rotary shaft (14) part is positioned at collection chamber (16) with collection chamber (16) mode of communicating, and hollow rotary shaft (14) is positioned on the part of collection chamber (16) hole or filtering net are set.
20, bio-reactor according to claim 11, it is characterized in that: described collection chamber (16) communicates with collecting storage tank (14) by collection tube (7), and collection tube (7) is provided with the collecting pump (8) that nutrient solution is pumped into collection storage tank (14) by collection chamber (16).
21, bio-reactor according to claim 11 is characterized in that: described culture tank (2) and sleeve (12) communicate by discharge nozzle (9) and feed back pipe (11); Wherein discharge nozzle (9) one end openings stretch into culture tank (2) bottom from culture tank (2) top, and the other end opening is positioned at sleeve (12) bottom, and discharge nozzle (9) is provided with the discharging pump (10) that nutrient solution is pumped into sleeve (12) by culture tank (2).
22, bio-reactor according to claim 21 is characterized in that: described feed back pipe (11) is provided with the feed back pump that nutrient solution is pumped into culture tank (2) by sleeve (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820122457 CN201296758Y (en) | 2008-09-09 | 2008-09-09 | Rotary filter for bioreactor and bioreactor comprising same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820122457 CN201296758Y (en) | 2008-09-09 | 2008-09-09 | Rotary filter for bioreactor and bioreactor comprising same |
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| Publication Number | Publication Date |
|---|---|
| CN201296758Y true CN201296758Y (en) | 2009-08-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200820122457 Expired - Fee Related CN201296758Y (en) | 2008-09-09 | 2008-09-09 | Rotary filter for bioreactor and bioreactor comprising same |
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| Country | Link |
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| CN (1) | CN201296758Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102408990A (en) * | 2010-09-25 | 2012-04-11 | 北京清大天一科技有限公司 | Stirring type animal cells culture bioreactor and its sealing method |
| CN102591384A (en) * | 2012-02-09 | 2012-07-18 | 金华华东环保设备有限公司 | Multilevel-linkage temperature and liquid level control device system |
| CN110016437A (en) * | 2019-04-26 | 2019-07-16 | 南京比瑞生物科技有限公司 | Vertical rotating tank bioreactor for large-scale culture cell |
-
2008
- 2008-09-09 CN CN 200820122457 patent/CN201296758Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102408990A (en) * | 2010-09-25 | 2012-04-11 | 北京清大天一科技有限公司 | Stirring type animal cells culture bioreactor and its sealing method |
| CN102408990B (en) * | 2010-09-25 | 2013-06-19 | 北京清大天一科技有限公司 | Stirring type animal cells culture bioreactor and its sealing method |
| CN102591384A (en) * | 2012-02-09 | 2012-07-18 | 金华华东环保设备有限公司 | Multilevel-linkage temperature and liquid level control device system |
| CN102591384B (en) * | 2012-02-09 | 2014-09-03 | 金华华东环保设备有限公司 | Multilevel-linkage temperature and liquid level control device system |
| CN110016437A (en) * | 2019-04-26 | 2019-07-16 | 南京比瑞生物科技有限公司 | Vertical rotating tank bioreactor for large-scale culture cell |
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