CN108220139A - A kind of difference position double helix stirring integratedization solid fermentation device - Google Patents
A kind of difference position double helix stirring integratedization solid fermentation device Download PDFInfo
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- CN108220139A CN108220139A CN201810237361.6A CN201810237361A CN108220139A CN 108220139 A CN108220139 A CN 108220139A CN 201810237361 A CN201810237361 A CN 201810237361A CN 108220139 A CN108220139 A CN 108220139A
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- 238000003756 stirring Methods 0.000 title claims abstract description 27
- 239000007787 solid Substances 0.000 title claims abstract description 25
- 238000000855 fermentation Methods 0.000 title claims abstract description 23
- 230000004151 fermentation Effects 0.000 title claims abstract description 23
- 125000004122 cyclic group Chemical group 0.000 claims description 3
- 239000003818 cinder Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 abstract description 8
- 239000002893 slag Substances 0.000 abstract description 8
- 238000000605 extraction Methods 0.000 abstract description 7
- 238000001816 cooling Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 6
- 230000010354 integration Effects 0.000 abstract description 6
- 102000004190 Enzymes Human genes 0.000 abstract description 5
- 108090000790 Enzymes Proteins 0.000 abstract description 5
- 230000008439 repair process Effects 0.000 abstract description 2
- 238000002156 mixing Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000010563 solid-state fermentation Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000000047 product Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 206010037660 Pyrexia Diseases 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003638 chemical reducing agent Substances 0.000 description 2
- 239000000498 cooling water Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000012257 stirred material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
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- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of poor position double helix stirring integratedization solid fermentation devices, including cylinder, double helix agitating paddle are equipped in the cylinder, the cylinder body bottom is equipped with the sieves bottom hole plate of 40 100 mesh, and the cylinder lower sides are equipped with slag notch.Cylinder body bottom of the present invention uses 40 100 mesh sieves bottom hole plates, increases the water-soluble extraction integration of solid enzyme, not easy to pollute comprehensively using seal operation；Using poor position double helix agitating paddle, small one and large one auger leaf misplaces 90 °, promotes the loosening degree of solid koji back and forth by rotating stirring, can speed up extension and the cooling effect of mycelia；By slag notch deslagging, slag tap and repair convenient for filtering.
Description
Technical field
The invention belongs to biological fermentation equipment manufacturing technology fields, and in particular to a kind of difference position double helix stirring integratedization is solid
Body installation for fermenting.
Background technology
Solid state fermentation is a kind of traditional zymotechnique, using inert carrier as supporter, the moisture content in medium of formation
Between 30-70%, without excessive Free water, produced suitable for the fungi microbe grown under low humidified state for producing metabolism
Object.Solid state fermentation easily generates a large amount of fermentation fever, mycelia sprawling causes to train due to fermenting under finer and close media environment
Base junction group is supported, there is viscosity, air transmits difficulty, is easy to cause inside culture medium in anaerobic state, is not easy to grow.Its metabolic heat
Removal when often resulting in problem, especially mass production, often limit its large-scale production capacity.In addition, pH value in solid fermentation,
Humidity, substrate concentration not easy-regulating, reproducibility is poor, and quality is unstable, is difficult to obtain the product of high-content.Especially extensive
Generally cause the amount of labour big, bent thickness cannot be too thick, and mycelia using aerated koji making or tray koji-making in production process
The problems such as extension and air, moisture transmit cannot be resolved.
The solid-state fermenter of the prior art has drum-type, has spherical shape, has cuboid song case, has internal stirred fermentor,
There is the stirred autoclave of double-screw type, rotating blade turns over the fermentation tanks such as song.Above-mentioned solid-state fermenter mixing is uneven, easily
Agglomerating, hardly possible heat dissipation although the design of some tanks keeps the skin wet or be passed through air in early period, leads to grow unbalanced, growth
Efficiency is low, easily pollutes.
Invention content
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of poor position double helix stirring integratedization solid hair
Ferment device, to solve the above problems.
The embodiment provides a kind of poor position double helix stirring integratedization solid fermentation device, including cylinder, cylinder
It is equipped with double helix agitating paddle in vivo, cylinder body bottom is equipped with the sieves bottom hole plate of 40-100 mesh, and cylinder lower sides are equipped with out
Cinder notch.
Further, double helix agitating paddle includes small stirrer paddle and big stirrer paddle, small stirrer paddle and big agitating paddle
Leaf misplaces 90 ° and arranges, big stirrer paddle is cyclic annular stirrer paddle.
Further, the width of the big stirrer paddle and small stirrer paddle is 40-60mm, outside the big agitating paddle leaf joint
The ratio of diameter and the barrel diameter is 2：3；The ratio of the small stirrer paddle outer diameter and the barrel diameter is 1：3；
Further, double helix agitating paddle distance from bottom sieves bottom hole plate 1-2cm.
Further, the side of double helix agitating paddle is furnished with scraper plate, barrel 10mm of the scraper plate outer end away from cylinder.
Compared with prior art the beneficial effects of the invention are as follows：
(1) by increasing the sieves bottom hole plate of 40-100 mesh (aperture 0.425-0.150mm) in solid-state fermenter, increase
Add the water-soluble extraction integration of solid enzyme, it is not easy to pollute comprehensively using seal operation.
(2) hypha body is formed based on fungus growth in solid ferment process, connects into the bulk of large area, generated a large amount of
Fermentation fever, the problem of causing heat-transfer effect bad, using poor position double helix stirring slurry, small one and large one auger blade, dislocation
90 ° of arrangements promote the loosening degree of solid koji by rotating stirring back and forth, accelerate extension and the cooling effect of mycelia, favorably
In industrialization, efficiency of pcr product is improved.
(3) high enzyme activity product is formed by solid fermentation, can be directly used using the device as mixing tank, directly addition extraction
It takes agent that extraction is mixed, coarse filtration liquid is directly filtered out under the action of pressurization by sieves bottom hole plate, effectively reduce pollution,
Integration operation is convenient for industrialization.
(4) by side slag notch, Production With Residue of Enzymatic Hydrolysis can quickly be removed.
Description of the drawings
Fig. 1 is a kind of structure diagram of poor position double helix stirring integratedization solid fermentation device of the present invention；
Fig. 2 is a kind of structural representation of poor position double helix stirring integratedization solid fermentation device double helix agitating paddle of the present invention
Figure.
Specific embodiment
The present invention is described in detail for shown each embodiment below in conjunction with the accompanying drawings, but it should explanation, these
Embodiment is not limitation of the present invention, those of ordinary skill in the art according to these embodiment institute work energy, method,
Or equivalent transformation or replacement in structure, all belong to the scope of protection of the present invention within.
Join shown in Fig. 1, a kind of poor position double helix stirring integratedization solid fermentation device is present embodiments provided, including support
Foot 1, steam loop, chuck ellipse head 3, ellipse head 4, sieves bottom hole plate 5, tetrafluoro scraper plate 6 (guarantee cylinder lateral wall
Material scrape off), jacket cylinder 7, cylinder 8, speed reducer 9, reductor shaft 91, double helix agitating paddle 10, small stirrer paddle 10,
Big stirrer paddle 102, slag notch 11, visor/lamp hole mouth 12, feed inlet 13, thermometer mouth/drain port 14, liquid outlet 15, cooling
Water inlet 16, cooling water outlet 17, steam port 18, cylinder 8 is interior to be equipped with double helix agitating paddle 10, and double helix agitating paddle 10 is by subtracting
Fast arbor 91 is connect with speed reducer 9, and 8 bottom of cylinder is equipped with the sieves bottom hole plate 5 of 40-100 mesh, is set on 8 lower sides of cylinder
There is slag notch 11.
Integration solid fermentation device provided in this embodiment, cylinder body bottom use 40-100 mesh (aperture 0.425-
Punching sieve plate 0.150mm) does staving bottom, increases the water-soluble extraction integration of solid enzyme, comprehensively using seal operation, no
Easy to pollute, fermentation tank (device) bottom is passed through air, and air transmission is carried out by sieves bottom hole plate, beneficial to stirring material dispersion
It is swapped with air, is conducive to the growth of aerial hyphae；By the mixing of stirring, contribute to prolonging for vegetative hyphae in mycelium
It stretches；Using poor position double helix agitating paddle, small one and large one auger leaf misplaces 90 °, and solid is promoted back and forth by rotating stirring
Bent loosening degree, can speed up extension and the cooling effect of mycelia；By (selecting larger) slag notch deslagging, convenient for filtering
It slags tap and repairs.
Join shown in Fig. 2, double helix agitating paddle 10 includes small stirrer paddle 101 and big stirrer paddle 102, small stirrer paddle
101 misplace 90 ° with big stirrer paddle 102 arranges, big stirrer paddle 102 is cyclic annular stirrer paddle.
In the present embodiment, 8 radius of cylinder is 300mm, and the width of small stirrer paddle 101 is 40mm, and stirring radius is
100mm, the ring outer radius of big stirrer paddle 102 is 200mm, and ring inside radius is 140mm, blade thickness 60mm.
In the present embodiment, bottom stirring blade and bottom angled degree are smaller, and 8 bottom of double helix agitating paddle is away from filtering sieve pore
Bottom plate 5 has 1-2cm, and being conducive to bottoms material can be stirred.The side of double helix agitating paddle 10 is furnished with tetrafluoro scraper plate 6, and tetrafluoro is scraped
Barrel 10mm of 6 outer end of plate away from cylinder 8.
The integrated solid-state fermentation tank provided through this embodiment makes ventilation culture, process cooling, double helix mixing, leaching
Carry, filter, cleaning and carried out all in hermetically sealed can, by pressurization, accelerated filtration carries out separation of solid and liquid, filtrate collect carry out it is next
Work is walked, filter residue is excluded from slag-drip opening.Fermentation process includes:It is trained in the low sterilising medium of water content with mould microorganism
It supports, improves the fast-growth of fungal hyphae by increasing humidity and moisture, air, promoted using poor position double helix agitating paddle
Mass transfer, the effect for passing oxygen are played in mixing, reduce fermentation fever, and adjusting is formed with secretes a large amount of metabolism enzyme product conducive to mould, carries
It is pure to carry out mixing extraction by adding in extractant, and pass through pressurization filtering on filtering sieve tray and form coarse filtration liquid.
It can be tuned into form different workshop sections according to production technology by the integration solid-state fermentation tank, material water mixing can be carried out,
High-temperature sterilization, gas supply culture, extracting technology can once be completed in the tank, reduce labour workload, reduce mycotic spore
Diffusion pollution, can effectively realize automation control.
Embodiment 1
Take wheat bran and water proportionally 1:0.5 dispensing, is added in feeding port, and startup is stirred material, is formed loose
Material.
Chuck is passed through steam sterilizing material, stops stirring when temperature reaches 95 DEG C, continues to add into tank by live (open) steam
Enter disinfection, temperature keeps the temperature 45min when reaching 121 DEG C.
Filtrated air cooling is passed through, is passed through jacket water (J.W.) cooling, when temperature drops to 100 DEG C in tank, starts stirring, it is quick mixed
Close heat dissipation.
When temperature of charge reaches 34 DEG C, seed can be added in sterilising medium by inoculation mouth, by reversely stirring
Mix seed mixture liquid.
Since solid-state fermentation tank has moisturizing effect, humid air is passed through by bottom, passes through the sieves bottom hole of bottom
Plate evenly spreads to culture medium, accelerates mycelial growth, at the same control early period culture 15hr in keep tank in temperature at 30 DEG C
Or so, temperature is at 28 DEG C in 18-32hr holding tanks.
To cultured wet triton, excessively positive stirring, releases from the slag-drip opening of side, for drying, can also pass through chuck
Heating and thermal insulation reduces the Qushui River point in fermentation tank, convenient for drying.
Extractant can also be added in, stirring extraction by pressurization, using sieves bottom hole plate slag-liquid separation, further divides
From purifying filtered fluid.
The cultivation temperature that the device is used according to the fungal strain adjustment moisture content relationship used and mould.
Those listed above is a series of to be described in detail only for feasibility embodiment of the invention specifically
Bright, they are not to limit the scope of the invention, all equivalent implementations made without departing from skill spirit of the present invention
Or change should all be included in the protection scope of the present invention.
It is obvious to a person skilled in the art that the present invention is not limited to the details of above-mentioned exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requirement rather than above description limit, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.
Claims (5)
1. a kind of difference position double helix stirring integratedization solid fermentation device, which is characterized in that including cylinder, be equipped in the cylinder
Double helix agitating paddle, the cylinder body bottom are equipped with the sieves bottom hole plate of 40-100 mesh, and the cylinder lower sides are equipped with out
Cinder notch.
2. a kind of poor position double helix stirring integratedization solid fermentation device according to claim 1, which is characterized in that described
Double helix agitating paddle includes small stirrer paddle and big stirrer paddle, and the small stirrer paddle misplaces 90 ° with the big stirrer paddle
Arrangement, the big stirrer paddle are cyclic annular stirrer paddle.
3. a kind of poor position double helix stirring integratedization solid fermentation device according to claim 2, which is characterized in that described
The width of big stirrer paddle and small stirrer paddle is the ratio of 40-60mm, the big agitating paddle leaf joint outer diameter and the barrel diameter
It is 2：3；The ratio of the small stirrer paddle outer diameter and the barrel diameter is 1：3.
4. a kind of poor position double helix stirring integratedization solid fermentation device according to claim 1, which is characterized in that described
Sieves bottom hole plate 1-2cm described in double helix agitating paddle distance from bottom.
5. a kind of poor position double helix stirring integratedization solid fermentation device according to claim 1, which is characterized in that described
It is furnished with scraper plate, barrel 10mm of the scraper plate outer end away from the cylinder in the side of double helix agitating paddle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810237361.6A CN108220139B (en) | 2018-03-22 | 2018-03-22 | Differential double-screw stirring integrated solid fermentation device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810237361.6A CN108220139B (en) | 2018-03-22 | 2018-03-22 | Differential double-screw stirring integrated solid fermentation device |
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| Publication Number | Publication Date |
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| CN108220139A true CN108220139A (en) | 2018-06-29 |
| CN108220139B CN108220139B (en) | 2020-09-18 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108531384A (en) * | 2018-07-13 | 2018-09-14 | 泸州品创科技有限公司 | Compound caproic acid bacteria solution installation for fermenting |
| CN109265296A (en) * | 2018-11-11 | 2019-01-25 | 李祥 | The preparation method of biological farmyard manure |
| CN112409039A (en) * | 2020-11-25 | 2021-02-26 | 武汉达鑫源有机肥有限责任公司 | Organic fertilizer stack type fermentation method taking mushroom dregs as substrate |
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| CN208182976U (en) * | 2018-03-22 | 2018-12-04 | 云南师范大学 | A kind of difference position double helix stirring integratedization solid fermentation device |
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2018
- 2018-03-22 CN CN201810237361.6A patent/CN108220139B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201577497U (en) * | 2009-12-02 | 2010-09-15 | 许朝辉 | Automatic soy sauce fermenting device |
| CN102199521A (en) * | 2011-04-07 | 2011-09-28 | 华东理工大学 | Enclosed solid state fermentation bioreactor |
| CN202246500U (en) * | 2011-09-29 | 2012-05-30 | 王天娇 | Blender with double-ribbon scrapers |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108531384A (en) * | 2018-07-13 | 2018-09-14 | 泸州品创科技有限公司 | Compound caproic acid bacteria solution installation for fermenting |
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| CN112409039A (en) * | 2020-11-25 | 2021-02-26 | 武汉达鑫源有机肥有限责任公司 | Organic fertilizer stack type fermentation method taking mushroom dregs as substrate |
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