CN108220139B

CN108220139B - Differential double-screw stirring integrated solid fermentation device

Info

Publication number: CN108220139B
Application number: CN201810237361.6A
Authority: CN
Inventors: 唐湘华; 黄遵锡; 李俊俊; 杨云娟; 许波; 慕跃林; 周峻沛
Original assignee: Yunnan Normal University
Current assignee: Yunnan Normal University
Priority date: 2018-03-22
Filing date: 2018-03-22
Publication date: 2020-09-18
Anticipated expiration: 2038-03-22
Also published as: CN108220139A

Abstract

The invention provides a differential double-screw stirring integrated solid fermentation device which comprises a barrel, wherein a double-screw stirring paddle is arranged in the barrel, a filtering sieve mesh bottom plate with 40-100 meshes is arranged at the bottom of the barrel, and a slag outlet is formed in the side wall of the lower part of the barrel. According to the invention, the bottom of the cylinder body adopts a 40-100-mesh filtering sieve pore bottom plate, so that the integration of solid enzyme water-soluble leaching is increased, the sealing operation is comprehensively adopted, and the pollution is not easy to occur; the differential double-helix stirring paddle is adopted, the stirring blades are arranged in a large and small mode and are staggered by 90 degrees, the loosening degree of the solid yeast is improved back and forth through forward and reverse stirring, and the extension and heat dissipation effects of hyphae can be accelerated; the slag is discharged through the slag outlet, so that the filtering, slag discharging and maintenance are convenient.

Description

Differential double-screw stirring integrated solid fermentation device

Technical Field

The invention belongs to the technical field of manufacturing of biological fermentation equipment, and particularly relates to a differential double-helix stirring integrated solid fermentation device.

Background

Solid state fermentation is a traditional fermentation process, uses an inert carrier as a support, forms a culture medium with the water content of 30-70%, does not contain excessive free water, and is suitable for producing metabolites by using fungus microorganisms growing under a low-humidity state. Solid state fermentation easily generates a large amount of fermentation heat due to fermentation in a relatively compact culture medium environment, hypha spreads to cause the culture medium to agglomerate, and the culture medium has viscosity and is difficult to transfer air, so that the inside of the culture medium is easily in an anaerobic state and is difficult to grow. The removal of the metabolic heat is often problematic, especially in high volume production, which often limits the large-scale production capacity. In addition, the pH value, humidity and substrate concentration in the solid fermentation are not easy to regulate, the reproducibility is poor, the quality is unstable, and a high-content product is not easy to obtain. Especially, the ventilation yeast making or tray yeast making is commonly adopted in the large-scale production process, which results in large labor amount, not too thick yeast thickness, and the problems of hypha extension, air and moisture transmission and the like which can not be solved.

The solid-state fermentation tank in the prior art has a drum type, a spherical shape, a cuboid curved box, an internal stirring fermentation tank, a double-spiral stirring reaction kettle, a rotating blade turning fermentation tank and the like. The solid-state fermentation tank is uneven in mixing and stirring, easy to agglomerate and difficult to radiate heat, and even though some tanks are designed to supplement moisture or introduce air in the early stage, the growth is unbalanced, the growth efficiency is low, and the pollution is easy.

Disclosure of Invention

The invention aims to provide a differential double-helix stirring integrated solid fermentation device aiming at overcoming the defects in the prior art, and aims to solve the problems.

The embodiment of the invention provides a differential double-screw stirring integrated solid fermentation device which comprises a barrel, wherein a double-screw stirring paddle is arranged in the barrel, a filtering sieve mesh bottom plate with 40-100 meshes is arranged at the bottom of the barrel, and a slag outlet is formed in the side wall of the lower part of the barrel.

Further, the double-helix stirring paddle comprises a small stirring paddle and a large stirring paddle, the small stirring paddle and the large stirring paddle are arranged in a staggered mode by 90 degrees, and the large stirring paddle is an annular stirring paddle.

Further, the width of the large stirring blade and the small stirring blade is 40-60mm, and the ratio of the outer diameter of the ring of the large stirring blade to the diameter of the cylinder body is 2: 3; the ratio of the outer diameter of the small stirring paddle to the diameter of the cylinder body is 1: 3;

furthermore, the bottom of the double-helix stirring paddle is 1-2cm away from the bottom plate of the filtering sieve pore.

Furthermore, a scraping plate is arranged on the side surface of the double-helix stirring paddle, and the distance between the outer end of the scraping plate and the wall of the cylinder body is 10 mm.

Compared with the prior art, the invention has the beneficial effects that:

(1) by adding a filtering sieve pore bottom plate with 40-100 meshes (the pore diameter is 0.425-0.150mm) in the solid state fermentation tank, the integration of solid enzyme water-soluble leaching is increased, the sealing operation is comprehensively adopted, and the pollution is not easy to occur.

(2) Based on the problem that the heat transfer effect is poor due to the fact that mycelial clusters are formed by growth of moulds in the solid-state fermentation process and are connected into large-area blocks to generate a large amount of fermentation heat, differential double-helix stirring paddles are adopted, large and small spiral stirring blades are arranged in a staggered mode by 90 degrees, the loosening degree of solid koji is improved back and forth through positive and negative rotation stirring, the extension and heat dissipation effects of the mycelial clusters are accelerated, industrialization is facilitated, and the product yield is improved.

(3) Form high enzyme activity product through solid fermentation, can directly regard as the compounding jar to use the device, directly add the extractant and mix the stirring extraction, directly filter out coarse filtration liquid under the pressurized effect through filtering the sieve mesh bottom plate, effective pollution abatement, the integrated operation is convenient for the industrialization.

(4) Through the side slag hole, the enzymolysis slag can be rapidly removed.

Drawings

FIG. 1 is a schematic structural diagram of a differential double-helix stirring integrated solid fermentation device of the present invention;

FIG. 2 is a schematic structural diagram of a double-helix stirring paddle of the differential double-helix stirring integrated solid fermentation device.

Detailed Description

The present invention is described in detail with reference to the embodiments shown in the drawings, but it should be understood that these embodiments are not intended to limit the present invention, and those skilled in the art should understand that functional, methodological, or structural equivalents or substitutions made by these embodiments are within the scope of the present invention.

Referring to fig. 1, the present embodiment provides a differential double helix stirring integrated solid fermentation device, which comprises a supporting leg 1, a steam ring, a jacket elliptical head 3, an elliptical head 4, a filtering sieve pore bottom plate 5, a tetrafluoro scraper 6 (ensuring that the materials on the side wall of the cylinder can be scraped off), a jacket cylinder 7, a cylinder 8, a speed reducer 9, a speed reducer shaft 91, and a double helix stirring paddle 10, the stirring device comprises a small stirring blade 10, a large stirring blade 102, a slag hole 11, a sight glass/lamp hole 12, a feeding hole 13, a thermometer hole/drain hole 14, a liquid outlet 15, a cooling water inlet 16, a cooling water outlet 17 and a steam port 18, wherein a double-helix stirring paddle 10 is arranged in a barrel 8, the double-helix stirring paddle 10 is connected with a speed reducer 9 through a speed reducer shaft 91, the bottom of the barrel 8 is provided with a filtering sieve pore bottom plate 5 with 40-100 meshes, and the side wall of the lower part of the barrel 8 is provided with the slag hole 11.

According to the integrated solid fermentation device provided by the embodiment, the bottom of the barrel body is made of the punching screen plate with the aperture of 40-100 meshes (the aperture is 0.425-0.150mm) as the bottom of the barrel body, so that the integration of solid enzyme water-soluble leaching is increased, the sealing operation is comprehensively adopted, the pollution is not easy to occur, air is introduced into the bottom of the fermentation tank (device), and the air is transferred through the bottom plate of the filtering screen hole, so that the material dispersion and the air exchange are favorably stirred, and the growth of aerial hyphae is favorably; mixing by stirring to help the extension of vegetative hyphae in the mycelium; the differential double-helix stirring paddle is adopted, the stirring blades are arranged in a large and small mode and are staggered by 90 degrees, the loosening degree of the solid yeast is improved back and forth through forward and reverse stirring, and the extension and heat dissipation effects of hyphae can be accelerated; slag is discharged through a (larger) slag outlet, so that the filtering, slag discharging and maintenance are convenient.

Referring to fig. 2, the double-helix stirring paddle 10 includes a small stirring paddle 101 and a large stirring paddle 102, the small stirring paddle 101 and the large stirring paddle 102 are arranged in a staggered manner by 90 °, and the large stirring paddle 102 is an annular stirring paddle.

In this embodiment, the radius of the cylinder 8 is 300mm, the width of the small stirring blade 101 is 40mm, the stirring radius is 100mm, the outer radius of the ring of the large stirring blade 102 is 200mm, the inner radius of the ring is 140mm, and the blade thickness is 60 mm.

In the embodiment, the inclination of the bottom stirring blade and the inclination of the bottom are smaller, and the distance between the bottom of the double-helix stirring paddle 8 and the bottom of the filtering sieve pore bottom plate 5 is 1-2cm, which is beneficial to stirring the bottom materials. The side surface of the double-helix stirring paddle 10 is provided with a tetrafluoro scraper 6, and the distance between the outer end of the tetrafluoro scraper 6 and the cylinder wall of the cylinder 8 is 10 mm.

Through the integration solid state fermentation jar that this embodiment provided, make that to ventilate cultivation, process cooling, double helix mixing, leaching, filtration, washing all go on in the sealed tank, through the pressurization, filter with higher speed, carry out solid-liquid separation, the filtrating is collected and is carried out next step work, and the filter residue is got rid of from row cinder notch. The fermentation process comprises culturing mould microorganism in a low water content sterilization culture medium, increasing humidity, water and air to promote the rapid growth of mould hyphae, mixing with a differential double helix stirring paddle to achieve mass transfer and oxygen transfer effects, reducing fermentation heat, regulating to form a product beneficial to mould secretion of a large amount of metabolic enzyme, purifying, adding an extractant to perform mixed extraction, and filtering on a filtering sieve plate under pressure to form a coarse filtrate.

The integrated solid fermentation tank can be adjusted to form different working sections according to the production process, can be used for mixing material and water, sterilizing at high temperature and culturing by air supply, can complete the extraction process in the tank at one time, reduces the labor workload, reduces the diffusion pollution of mould spores, and can effectively realize automatic control.

Example 1

The wheat bran and the water are mixed according to the proportion of 1:0.5, added into a feeding port, and stirred to mix the materials to form loose materials.

Steam is introduced into the jacket to sterilize the materials, stirring is stopped when the temperature reaches 95 ℃, the materials are continuously added for sterilization through direct steam entering the tank, and the temperature is kept for 45min when the temperature reaches 121 ℃.

Sterile air is introduced for cooling, jacket water is introduced for cooling, and when the temperature in the tank is reduced to 100 ℃, stirring is started, and rapid mixing and heat dissipation are carried out.

When the temperature of the material reached 34 ℃, the seeds were added to the sterile medium through the inoculation port and the seed solution was mixed by reverse stirring.

Since the solid fermentation tank has moisture keeping effect, moist air is introduced through the bottom, and uniformly dispersed into the culture medium through the bottom plate of the filtering mesh at the bottom, so as to accelerate the growth of mycelia, and simultaneously, the temperature in the tank is kept at about 30 ℃ within 15hr of pre-culture, and the temperature in the tank is kept at 28 ℃ within 18-32 hr.

The cultured wet koji is discharged from a residue discharge port on the side surface through positive stirring for drying, and the koji can also be heated and insulated through a jacket to reduce koji moisture in a fermentation tank, thereby being convenient for drying.

Or adding an extracting agent, stirring and extracting, pressurizing, separating slag and liquid by using a bottom plate of a filtering sieve pore, and further separating and purifying the filtrate.

The device adjusts the water proportion relation and the culture temperature of the mould according to the used mould strain.

The above-listed detailed description is only a specific description of a possible embodiment of the present invention, and they are not intended to limit the scope of the present invention, and equivalent embodiments or modifications made without departing from the technical spirit of the present invention should be included in the scope of the present invention.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims

1. The differential double-screw stirring integrated solid fermentation device is characterized by comprising a barrel, wherein a double-screw stirring paddle is arranged in the barrel, a filtering sieve mesh bottom plate with 40-100 meshes is arranged at the bottom of the barrel, and a slag outlet is formed in the side wall of the lower part of the barrel;

the double-helix stirring paddle comprises a small stirring paddle and a large stirring paddle, the small stirring paddle and the large stirring paddle are arranged in a staggered mode by 90 degrees, and the large stirring paddle is an annular stirring paddle;

the bottom of the double-helix stirring paddle is 1-2cm away from the bottom plate of the filtering sieve pore; the filtering sieve pore base plate is used for directly filtering out coarse filtrate under the action of pressurization; the width of the large stirring blade and the small stirring blade is 40-60mm, and the ratio of the outer diameter of the large stirring blade ring to the diameter of the cylinder body is 2: 3; the ratio of the outer diameter of the small stirring paddle to the diameter of the cylinder body is 1: 3; and a scraping plate is arranged on the side surface of the double-helix stirring paddle, and the distance between the outer end of the scraping plate and the wall of the barrel is 10 mm.