CN109777730A - A kind of culture sampler and application method for lake water bacterium - Google Patents
A kind of culture sampler and application method for lake water bacterium Download PDFInfo
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- CN109777730A CN109777730A CN201910127680.6A CN201910127680A CN109777730A CN 109777730 A CN109777730 A CN 109777730A CN 201910127680 A CN201910127680 A CN 201910127680A CN 109777730 A CN109777730 A CN 109777730A
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- lake water
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- probe tube
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Abstract
The invention discloses a kind of culture samplers and application method for lake water bacterium, including sampling cabinet, agitator tank and incubator, sampling cabinet bottom is equipped with rotating base, upper end is equipped with through-hole on the left of sampling cabinet, through hole is equipped with probe tube, it samples bottom of the tube and is equipped with sampling head, probe tube is connect with sampling pump, impurity filtering net is installed inside sampling cabinet, sampling cabinet bottom right side is equipped with leakage fluid dram, connecting flange is installed at leakage fluid dram, connecting flange connected drainage pipe, agitator tank is mounted on the right side of sampling cabinet, drain pipe is connected to agitator tank, agitator tank connects incubator by communicating pipe, structure of the invention principle is simple, it can be realized the quick sampling to lake water, uniform stirring and constant temperature incubation, it can be improved the culture efficiency of lake water bacterium, further improve analysis efficiency and analysis precision.
Description
Technical field
The present invention relates to lake water Bacteria Culture technical field, specially a kind of culture sampler for lake water bacterium and
Application method.
Background technique
Bacterium is one of the main groups of biology, belongs to bacterium domain.It is also most one kind of quantity in all biologies, according to estimating
Meter, there are about 5 × 10^30 for sum.The shape of bacterium is quite various, mainly there is spherical, rod-shaped and helical form.Bacterium
Have a great impact to mankind's activity.On the one hand, bacterium is the pathogen of many diseases, including pulmonary tuberculosis, stranguria syndrome, anthracnose,
The diseases such as syphilis, the plague, sand holes are caused by bacterium.However, the mankind also utilize bacterium often, for example, cheese and Yoghourt and
The production of fermented glutinous rice, the manufacture of part antibiotic, processing of waste water etc., it is all related with bacterium.In technical field of biology, bacterium has
Also extensive use is write.Bacteria Culture is a kind of technology for by artificial means breeding bacterial growth.Bacterium is divided in nature
Cloth is extremely wide, and quantity is big, and type is more, it can promote the well-being of mankind, and can also become pathogenic reason.Most of bacteriums can be manually square
Method culture, that is, be inoculated on culture medium, its growth and breeding is made.The bacterium come is turned out for studying, identifying and applying.Carefully
Bacterium culture is a complicated technology.Existing lake water bacterial sampling apparatus structure is complicated, and cumbersome, and therefore, having must
It improves.
Summary of the invention
The purpose of the present invention is to provide a kind of culture samplers and application method for lake water bacterium, on solving
State the problem of proposing in background technique.
To achieve the above object, the invention provides the following technical scheme: a kind of culture sampler for lake water bacterium,
Including sampling cabinet, agitator tank and incubator, the sampling cabinet bottom is equipped with rotating base, and upper end is equipped on the left of the sampling cabinet
Through-hole, the through hole are equipped with probe tube, and the sampling bottom of the tube is equipped with sampling head, and the probe tube is connect with sampling pump,
Impurity filtering net is installed, the sampling cabinet bottom right side is equipped with leakage fluid dram, installs at the leakage fluid dram inside the sampling cabinet
There are connecting flange, the connecting flange connected drainage pipe, the agitator tank is mounted on the right side of sampling cabinet, and the drain pipe connection is stirred
Case is mixed, the agitator tank connects incubator by communicating pipe；
Rabbling mechanism is equipped in the agitator tank, the rabbling mechanism includes agitating shaft, paddle and stirring motor, the stirring
Motor is mounted at the top of agitator tank, and the agitating shaft is mounted on inside agitator tank, and stirring motor, institute are connected at the top of the agitating shaft
It states paddle and is fixedly mounted on agitating shaft outer wall, the paddle uses arc paddle, is provided on the paddle multiple logical
Hole, the agitator tank bottom are equipped with tapered discharging hole.
Preferably, the incubator intracavity bottom is equipped with culture dish, and the culture dish is colorless and transparent pyrex
It constitutes, the incubator inner cavity top is equipped with headlamp, and the headlamp is set to the surface of culture dish, the incubator
Side wall is equipped with temperature sensor, and the culture dish bottom is equipped with heated at constant temperature heating wire and temperature controller, the constant temperature
Heating heating wire, temperature sensor are separately connected temperature controller.
Preferably, the impurity filtering net includes screen frame, the first strainer and the second strainer, and first strainer and second filter
Net is respectively and fixedly installed in screen frame, and first sieve meshes are less than the second sieve meshes.
Preferably, the rotating base includes upper pedestal and lower base, and it is convex to be equipped with the first hemispherical on the inside of the upper pedestal
It rises, is equipped with and symmetrical second hemispherical projections of the first hemispherical projections, first hemispherical projections on the inside of the lower base
And second be equipped with axis hole between hemispherical projections, is equipped with rotary shaft in the axis hole.
Preferably, the probe tube uses elastic telescopic probe tube, is equipped with flow control valve on the probe tube, and
Water depth sensor is also equipped in the sampling pipe outer wall.
Preferably, the drain pipe uses elastic telescopic tube body.
Preferably, application method the following steps are included:
A, probe tube is protruded into lake water, opens sampling pump later, sample pump work, probe tube is sampled lake water；
B, the lake water after sampling enters in sampling cabinet, and lake water carries out sampling cabinet bottom after contaminant filter net filtration；
C, the lake water after sampling is discharged into agitator tank by drain pipe；
D, rabbling mechanism is opened, agitating shaft rotation drives paddle rotation, and paddle carries out uniform stirring to sampling lake water；
E, the lake water after uniform stirring passes through in the culture dish that is discharged into communicating pipe in incubator；
F, heated at constant temperature electric-heating-wire-heating is controlled by controller later, heated at constant temperature heating wire carries out the lake water in culture dish
Heated at constant temperature, the bacteria culture media in culture dish carry out fast culture to the bacterium in lake water.
Compared with prior art, the beneficial effects of the present invention are:
(1) structure of the invention principle is simple, can be realized quick sampling, uniform stirring and constant temperature incubation to lake water, Neng Gouti
The culture efficiency of gaolu water bacteria, further improves analysis efficiency and analysis precision.
(2) agitator tank that the present invention uses can carry out uniform stirring to the lake water of collection, can be improved subsequent bacterium
Analysis precision.
(3) rotating base that the present invention uses can be realized 360 ° of any rotations, can be realized the rotation control to incubator
System, it is convenient that lake water in different zones is sampled.
(4) in the present invention, the impurity filtering net installed in sampling cabinet, which can be realized, is filtered sampling lake impurities in water,
Follow-up cultivation is facilitated to detect.
Detailed description of the invention
Fig. 1 is schematic structural view of the invention；
Fig. 2 is rotary base structure schematic diagram of the present invention；
Fig. 3 is impurity filtering net side view of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1-3 is please referred to, the present invention provides a kind of technical solution: a kind of culture sampler for lake water bacterium, packet
Sampling cabinet 1, agitator tank 2 and incubator 3 are included, 1 bottom of sampling cabinet is equipped with rotating base 4, the 1 left side upper end of sampling cabinet
Equipped with through-hole, the through hole is equipped with probe tube 5, and 5 bottom of probe tube is equipped with sampling head 6, the probe tube 5 and sampling
Pump 7 connects, and impurity filtering net 8 is equipped with inside the sampling cabinet 1, and 1 bottom right side of sampling cabinet is equipped with leakage fluid dram, the row
Connecting flange 9, the 9 connected drainage pipe 10 of connecting flange are installed, drain pipe 10 uses elastic telescopic tube body at liquid mouth；Institute
It states agitator tank 2 and is mounted on 1 right side of sampling cabinet, the drain pipe 10 is connected to agitator tank 2, and the agitator tank 2 is by connecting communicating pipe 11
Connect incubator 3；Wherein, probe tube 5 uses elastic telescopic probe tube, is equipped with flow control valve 31 on the probe tube 5, and
Water depth sensor 32 is also equipped on 5 outer wall of probe tube, the probe tube of use can be realized length adjustment, and flow tune
Section valve can be realized the adjusting to water flow, and water depth sensor can acquire the depth of sample water.
It is equipped with rabbling mechanism in the agitator tank 2, the rabbling mechanism includes agitating shaft 12, paddle 13 and stirring motor
14, the stirring motor 14 is mounted on 2 top of agitator tank, and the agitating shaft 12 is mounted on inside agitator tank 2, the agitating shaft 12
Top connects stirring motor 14, and the paddle 13 is fixedly mounted on 12 outer wall of agitating shaft, and the paddle 13 is stirred using arc
Leaf is mixed, multiple through-holes 15 are provided on the paddle 13,2 bottom of agitator tank is equipped with tapered discharging hole 16.The present invention uses
Agitator tank can to the lake water of collection carry out uniform stirring, can be improved subsequent bacterial analysis precision.
In the present invention, 3 intracavity bottom of incubator is equipped with culture dish 17, and the culture dish 17 is colorless and transparent borosilicic acid glass
Glass is constituted, and 3 inner cavity top of incubator is equipped with headlamp 18, and the headlamp 18 is set to the surface of culture dish 17, institute
The side wall for stating incubator 3 is equipped with temperature sensor 19, and 17 bottom of culture dish is equipped with heated at constant temperature heating wire 20 and temperature
Controller 21 is spent, the heated at constant temperature heating wire 20, temperature sensor 19 are separately connected temperature controller 21.
In the present invention, impurity filtering net 8 includes screen frame 22, the first strainer 23 and the second strainer 24, first strainer 23
It is respectively and fixedly installed in screen frame 22 with the second strainer 24,23 mesh of the first strainer is less than 24 mesh of the second strainer.This hair
In bright, the impurity filtering net installed in sampling cabinet, which can be realized, is filtered sampling lake impurities in water, and follow-up cultivation is facilitated to examine
It surveys.
In the present invention, rotating base 4 includes upper pedestal 25 and lower base 26, is equipped with the first hemisphere on the inside of the upper pedestal 25
Shape protrusion 27, be equipped on the inside of the lower base 26 with symmetrical second hemispherical projections 28 of the first hemispherical projections 27, described the
It is equipped with axis hole 29 between one hemispherical projections 27 and the second hemispherical projections 28, rotary shaft 30 is installed in the axis hole 29.This
The rotating base that invention uses can be realized 360 ° of any rotations, can be realized the rotation control to incubator, convenient to difference
Lake water in region is sampled.
Working principle: application method of the invention the following steps are included:
A, probe tube is protruded into lake water, opens sampling pump later, sample pump work, probe tube is sampled lake water；
B, the lake water after sampling enters in sampling cabinet, and lake water carries out sampling cabinet bottom after contaminant filter net filtration；
C, the lake water after sampling is discharged into agitator tank by drain pipe；
D, rabbling mechanism is opened, agitating shaft rotation drives paddle rotation, and paddle carries out uniform stirring to sampling lake water；
E, the lake water after uniform stirring passes through in the culture dish that is discharged into communicating pipe in incubator；
F, heated at constant temperature electric-heating-wire-heating is controlled by controller later, heated at constant temperature heating wire carries out the lake water in culture dish
Heated at constant temperature, the bacteria culture media in culture dish carry out fast culture to the bacterium in lake water.
In conclusion structure of the invention principle is simple, quick sampling, uniform stirring and the constant temperature training to lake water can be realized
It supports, can be improved the culture efficiency of lake water bacterium, further improve analysis efficiency and analysis precision.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of culture sampler for lake water bacterium, including sampling cabinet (1), agitator tank (2) and incubator (3), special
Sign is: sampling cabinet (1) bottom is equipped with rotating base (4), and upper end is equipped with through-hole on the left of the sampling cabinet (1), described
Through hole is equipped with probe tube (5), and probe tube (5) bottom is equipped with sampling head (6), the probe tube (5) and sampling pump (7)
Connection, the sampling cabinet (1) is internal to be equipped with impurity filtering net (8), and sampling cabinet (1) bottom right side is equipped with leakage fluid dram, institute
It states and is equipped at leakage fluid dram connecting flange (9), connecting flange (9) the connected drainage pipe (10), the agitator tank (2) is mounted on
On the right side of sampling cabinet (1), the drain pipe (10) is connected to agitator tank (2), and the agitator tank (2) passes through communicating pipe (11) connection culture
Case (3)；
Rabbling mechanism is equipped in the agitator tank (2), the rabbling mechanism includes agitating shaft (12), paddle (13) and stirring electricity
Machine (14), the stirring motor (14) are mounted at the top of agitator tank (2), and the agitating shaft (12) is mounted on agitator tank (2) inside,
Stirring motor (14) are connected at the top of the agitating shaft (12), the paddle (13) is fixedly mounted on agitating shaft (12) outer wall, institute
It states paddle (13) and uses arc paddle, be provided with multiple through-holes (15) on the paddle (13), agitator tank (2) bottom
Equipped with tapered discharging hole (16).
2. a kind of culture sampler for lake water bacterium according to claim 1, it is characterised in that: the incubator
(3) intracavity bottom is equipped with culture dish (17), and the culture dish (17) is colorless and transparent pyrex composition, the incubator
(3) inner cavity top is equipped with headlamp (18), and the headlamp (18) is set to the surface of culture dish (17), the incubator
(3) side wall is equipped with temperature sensor (19), and culture dish (17) bottom is equipped with heated at constant temperature heating wire (20) and temperature
It spends controller (21), the heated at constant temperature heating wire (20), temperature sensor (19) are separately connected temperature controller (21).
3. a kind of culture sampler for lake water bacterium according to claim 1, it is characterised in that: the impurity mistake
Strainer (8) includes screen frame (22), the first strainer (23) and the second strainer (24), first strainer (23) and the second strainer (24)
It is respectively and fixedly installed in screen frame (22), the first strainer (23) mesh is less than the second strainer (24) mesh.
4. a kind of culture sampler for lake water bacterium according to claim 1, it is characterised in that: the rotation bottom
Seat (4) includes upper pedestal (25) and lower base (26), is equipped with the first hemispherical projections (27) on the inside of the upper pedestal (25), described
It is equipped with and symmetrical second hemispherical projections (28) of the first hemispherical projections (27), first hemispherical on the inside of lower base (26)
Axis hole (29) are equipped between raised (27) and the second hemispherical projections (28), rotary shaft (30) are installed in the axis hole (29).
5. a kind of culture sampler for lake water bacterium according to claim 1, it is characterised in that: the probe tube
(5) elastic telescopic probe tube is used, is equipped with flow control valve (31) on the probe tube (5), and the probe tube (5) is outside
Water depth sensor (32) are also equipped on wall.
6. a kind of culture sampler for lake water bacterium according to claim 1, it is characterised in that: the drain pipe
(10) elastic telescopic tube body is used.
7. realizing a kind of application method of culture sampler for lake water bacterium described in claim 1, it is characterised in that:
Its application method the following steps are included:
A, probe tube is protruded into lake water, opens sampling pump later, sample pump work, probe tube is sampled lake water；
B, the lake water after sampling enters in sampling cabinet, and lake water carries out sampling cabinet bottom after contaminant filter net filtration；
C, the lake water after sampling is discharged into agitator tank by drain pipe；
D, rabbling mechanism is opened, agitating shaft rotation drives paddle rotation, and paddle carries out uniform stirring to sampling lake water；
E, the lake water after uniform stirring passes through in the culture dish that is discharged into communicating pipe in incubator；
F, heated at constant temperature electric-heating-wire-heating is controlled by controller later, heated at constant temperature heating wire carries out the lake water in culture dish
Heated at constant temperature, the bacteria culture media in culture dish carry out fast culture to the bacterium in lake water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910127680.6A CN109777730A (en) | 2019-02-20 | 2019-02-20 | A kind of culture sampler and application method for lake water bacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910127680.6A CN109777730A (en) | 2019-02-20 | 2019-02-20 | A kind of culture sampler and application method for lake water bacterium |
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| Publication Number | Publication Date |
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| CN109777730A true CN109777730A (en) | 2019-05-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910127680.6A Pending CN109777730A (en) | 2019-02-20 | 2019-02-20 | A kind of culture sampler and application method for lake water bacterium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110639034A (en) * | 2019-09-19 | 2020-01-03 | 泉州台商投资区春凯机械科技有限公司 | Solid state fermentation culture medium sterilization equipment |
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| CN207497198U (en) * | 2017-12-02 | 2018-06-15 | 张伟 | A kind of nurse, which relieves, uses clinical thermometer management box |
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2019
- 2019-02-20 CN CN201910127680.6A patent/CN109777730A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183382A (en) * | 2011-02-19 | 2011-09-14 | 中国科学院广州地球化学研究所 | Water passive sampler of pollutants |
| CN205398603U (en) * | 2016-02-16 | 2016-07-27 | 张嘉璐 | Incubator |
| CN206701802U (en) * | 2017-04-28 | 2017-12-05 | 广元市海鹏生物科技有限公司 | A kind of resin reverse washing tank |
| CN107058074A (en) * | 2017-06-18 | 2017-08-18 | 合肥职业技术学院 | A kind of packaged type microorganism classification sampling, culture and detection integrated apparatus |
| CN107966323A (en) * | 2017-11-20 | 2018-04-27 | 李正梅 | Water quality monitoring equipment with spot sampling structure |
| CN207497198U (en) * | 2017-12-02 | 2018-06-15 | 张伟 | A kind of nurse, which relieves, uses clinical thermometer management box |
| CN208254869U (en) * | 2018-04-15 | 2018-12-18 | 惠州东江威立雅环境服务有限公司 | Environment monitoring sewage sampling device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110639034A (en) * | 2019-09-19 | 2020-01-03 | 泉州台商投资区春凯机械科技有限公司 | Solid state fermentation culture medium sterilization equipment |
| CN110639034B (en) * | 2019-09-19 | 2021-10-08 | 北京炳洋科技有限公司 | Solid state fermentation culture medium sterilization equipment |
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Application publication date: 20190521 |