Solid state fermentation culture medium sterilization equipment
Technical Field
The invention relates to the field of culture medium sterilization, in particular to a sterilization device for a solid fermentation culture medium.
Background
When various fungi are cultured by the solid fermentation medium, the solid fermentation medium needs to be killed or organisms in materials and equipment are removed in advance by adopting a physical or chemical method, the proliferation of microorganisms which are unfavorable for the fungi is reduced, a layer of film is easily formed around cells when the contents of fat, powdered sugar and protein in the medium are higher in the sterilization process, even some foams are formed, namely a heat insulation layer is formed, the heat transfer is difficult, and the heat is difficult to penetrate through to kill and protect the microorganisms.
Disclosure of Invention
Aiming at the defects of the prior art, the invention is realized by the following technical scheme: the utility model provides a solid state fermentation culture medium sterilization apparatus, its structure includes motor, pivot, sampling tube, thermometer interface, thermoelectric lotus root interface, cooling tube, impeller, air-supply line, jar body, the motor is installed to jar body top one side, the corresponding jar internal mounting of motor has the sampling tube, install the cooling tube on the corresponding jar body of sampling tube bottom, the jar body on cooling tube top is improved level and is opened there is a thermometer interface, installs the air-supply line with the corresponding jar body opposite side of sampling tube, install the pivot in the middle of jar internal portion is positive, the pivot bottom is provided with impeller, impeller comprises center pin, blade, center pin surface both sides horizontal installation has the blade, blade and center pin movable mounting cooperation, install the entrance to a cave more than two on the blade.
As a further optimization of the technical scheme, the elimination wheels are installed in the hole and are arranged in a straight line.
As the further optimization of this technical scheme, eliminate the wheel and constitute the structure of a fretwork form by fixing clip, annular frame, spliced pole, linking post, annular frame surface level is equipped with the fixing clip, the fixing clip is fixed with annular frame, annular frame is fixed through the spliced pole more than two and linking post, linking post is equipped with more than two, and these link up the post and become even equidistance distribution inside annular frame, and adjacent two link up the post and pass through spliced pole horizontally connect together.
As a further optimization of the technical scheme, the vertical upper end and the vertical lower end of the connecting column are both provided with a connecting notch, a cone is in threaded connection in the connecting notch, and the cone is in a structure with a narrow upper part and a wide lower part.
As a further optimization of the technical scheme, triangular prisms are arranged on the front end face and the back end face of the tail end of the blade far away from the central shaft, and the triangular prisms and the blade are fixed.
As a further optimization of the technical scheme, two rows of triangular ridges are arranged on the side surface of the central shaft close to the bottom of the blade, more than two rows of triangular ridges are arranged in each row, the two rows of triangular ridges are centrosymmetric about the central axis of the blade, and the triangular ridges and the central shaft are horizontally fixed.
As a further optimization of the technical scheme, the extending direction of the triangular spines is parallel to the central axis of the blades.
Advantageous effects
Compared with the prior art, the solid fermentation culture medium sterilization equipment has the following advantages:
1. according to the invention, the culture medium material in the tank body can be scattered through the holes on the blades in the rotating process, so that a layer of film or even some foams can be prevented from being formed around cells in the solid fermentation medium, namely, a heat insulation layer is formed.
2. The blades of the invention can be provided with the eliminating wheels, the blades are scattered by the hollow-out eliminating wheels, the connecting columns which are uniformly arranged at equal intervals are scattered uniformly when the eliminating wheels are scattered, and the scattering effect is improved.
3. According to the invention, the triangular prism on the blade and the conical design on the eliminating wheel reduce the contact area with the solid fermentation culture medium, increase the pressure, and are easier to penetrate into the culture medium, so that the scattering effect is improved.
4. According to the triangular prism, when the triangular spines come out from the other side face of the blades, the triangular spines are contacted with the materials of the culture mediums again and are broken up again, and the broken culture mediums are not easy to clamp between the triangular spines and the blades because the triangular spines and the blades are parallel to each other, so that the culture mediums can fall off conveniently.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic sectional view of a solid fermentation medium sterilizing apparatus according to the present invention.
Fig. 2 is a schematic structural view of the stirring impeller of the present invention.
Fig. 3 is a schematic plan view of an elimination wheel of the present invention.
Fig. 4 is a schematic perspective view of the elimination wheel of the present invention.
Fig. 5 is a schematic perspective view of the cone of the present invention.
Fig. 6 is a schematic top view of a stirring impeller according to the present invention.
FIG. 7 is an enlarged view of the structure A in FIG. 6 according to the present invention.
In the figure: the device comprises a motor 1, a rotating shaft 2, a sampling tube 3, a thermometer interface 4, a thermoelectric lotus root interface 5, a cooling tube 6, a stirring impeller 7, an air inlet pipe 8, a tank body 9, a central shaft 7-1, blades 7-2, an eliminating wheel 7-3, a fixing clamp a, an annular outer frame b, a connecting column c, a connecting column d, a cone e, a triangular prism g and a triangular ridge f.
Detailed Description
In order to make the technical means, the original characteristics, the achieved purposes and the effects of the invention easy to understand, the following description and the accompanying drawings further illustrate the preferred embodiments of the invention.
Examples
Referring to fig. 1-7, the present invention provides a sterilization apparatus for a solid fermentation medium, which structurally comprises a motor 1, a rotating shaft 2, a sampling tube 3, a thermometer port 4, a thermoelectric coupling port 5, a cooling tube 6, a stirring impeller 7, an air inlet tube 8 and a tank 9, wherein the motor 1 is installed on one side of the top end of the tank 9, the sampling tube 3 is installed inside the tank 9 corresponding to the motor 1, the cooling tube 6 is installed on the tank 9 corresponding to the bottom of the sampling tube 3, the thermometer port 4 is horizontally arranged on the tank 9 at the top end of the cooling tube 6, the air inlet tube 8 is installed on the other side of the tank 9 corresponding to the sampling tube 3, the rotating shaft 2 is installed in the middle inside the tank 9, and the stirring impeller 7 is installed at the bottom of the rotating shaft 2.
The stirring impeller 7 is composed of a central shaft 7-1 and blades 7-2, the blades 7-2 are horizontally arranged on two sides of the outer surface of the central shaft 7-1, the blades 7-2 are movably arranged and matched with the central shaft 7-1, and more than two holes are formed in the blades 7-2.
The elimination wheels 7-3 are arranged in the hole, and the elimination wheels 7-3 are arranged in a straight line.
The eliminating wheel 7-3 is of a hollow structure consisting of a fixing clamp a, an annular outer frame b, connecting columns c and linking columns d, the fixing clamp a is arranged on the outer surface of the annular outer frame b in a horizontal mode, the fixing clamp a is fixed with the annular outer frame b, the annular outer frame b is fixed with the linking columns d through the connecting columns c more than two, the linking columns d are distributed in the annular outer frame b in an even and equidistant mode, the adjacent two linking columns d are connected together through the connecting columns c in a horizontal mode, the hollow design of the eliminating wheel 7-3 improves the direct scattering effect of the culture medium, and the materials of the culture medium are scattered more.
The upper end and the lower end of the linking column d are both provided with a linking notch, a cone e is connected with the linking notch through threads, the cone e is in narrow upper part and wide lower part, and is firstly contacted with the culture base material, so that the culture base material is more easily scattered relative to the end surface of the cylinder, and a heat insulation layer is prevented from being formed.
The triangular prisms g are arranged on the front end face and the back end face of the tail end of the blade 7-2 far away from the central shaft 7-1, the triangular prisms g and the blade 7-2 are fixed, a solid-state fermentation culture medium is not prone to deep penetration due to long-term stacking, the edges of the triangular prisms g are firstly in contact with materials of the culture medium, the contact area is small, and the triangular prisms g are easy to deep and more convenient to break up.
The side surface of the central shaft 7-1, which is close to the bottom of the blade 7-2, is provided with two rows of triangular spines f, each row is provided with more than two triangular spines, the two rows of triangular spines f are centrosymmetric about the central axis of the blade 7-2, the triangular spines f and the central shaft 7-1 are horizontally fixed, and the culture medium material passing through the eliminating wheel 7-3 is punctured, so that the culture medium material is further scattered, and the heat shield is prevented from being turned over again.
The extension direction of the triangular spines f is parallel to the central axis of the blades 7-2, and scattered culture medium materials are not easy to block in the space of the horizontal positions of the blades 7-2 and the triangular spines f.
Putting a solid fermentation culture medium into a tank body 9, wherein a layer of film is formed around cells more easily when the content of fat, powdered sugar and protein in the culture medium is higher in the sterilization process, the rotating shaft 2 rotates in the high-temperature sterilization process, the blade 7-2 also rotates along with the rotation, and the hollow-out elimination wheel 7-3 can gradually break up the film formed by high temperature in the culture medium when the solid fermentation culture medium is broken up in the rotation process of the hole on the blade 7-2, and can also be arranged in the hole, and the hollow-out elimination wheel 7-3 can break up the culture medium material more easily relative to a single hole and is more unfavorable for forming a heat insulation layer of the solid fermentation culture medium, in addition, the engagement columns d which are uniformly and equidistantly distributed can uniformly separate the material of the culture medium to break up the material of the culture medium more easily, so as to improve the damage effect of the heat insulation layer, the upper surface and the lower surface of the connecting column d are both provided with cones e, the upper surfaces are narrow and the lower surfaces are wide, no matter the cones e rotate forwards or reversely, the tips of the cones e are firstly contacted with the culture medium materials, and can more easily go deep into the solid fermentation culture medium relative to the end surface of the cylinder, the convenience of scattering the stirring impeller 7 is improved, the scattered culture medium materials come out from the other side surface of the blade 7-2, when the side surface comes out, the triangular spines f are contacted with the culture medium materials again and are scattered again, because the triangular spines f are parallel to the blade 7-2, the scattered culture medium materials are not easy to be clamped between the two, the culture medium materials are convenient to fall off, in the process, the culture medium materials passing through the impeller 7-3 are eliminated, the culture medium materials are further scattered, and the triangular prisms g on the blade 7-2 can also penetrate into the culture medium and other, preventing the reforming of the heat shield.
While there have been shown and described what are at present considered the fundamental principles of the invention, the essential features and advantages thereof, it will be understood by those skilled in the art that the present invention is not limited by the embodiments described above, which are merely illustrative of the principles of the invention, but rather, is capable of numerous changes and modifications in various forms without departing from the spirit or essential characteristics thereof, and it is intended that the invention be limited not by the foregoing descriptions, but rather by the appended claims and their equivalents.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.