CN212357263U - Cell culture device with monitoring function - Google Patents
Cell culture device with monitoring function Download PDFInfo
- Publication number
- CN212357263U CN212357263U CN202020578299.XU CN202020578299U CN212357263U CN 212357263 U CN212357263 U CN 212357263U CN 202020578299 U CN202020578299 U CN 202020578299U CN 212357263 U CN212357263 U CN 212357263U
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- culture
- test tube
- sleeve
- cultivateing
- cell culture
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- 238000004113 cell culture Methods 0.000 title claims abstract description 25
- 238000012544 monitoring process Methods 0.000 title claims abstract description 16
- 238000012360 testing method Methods 0.000 claims abstract description 28
- 239000002351 wastewater Substances 0.000 claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims description 26
- 238000007789 sealing Methods 0.000 claims description 11
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 3
- 230000010354 integration Effects 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 238000010367 cloning Methods 0.000 description 3
- 210000003437 trachea Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
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Abstract
The utility model discloses a cell culture device that possesses monitoring function, including cultivateing the dish, the bottom integration of cultivateing the dish is connected with waste water storage disc, longitudinal symmetry and interval equidistance distribute have the cultivation test tube in the inner chamber of cultivation dish, the center department of cultivateing the test tube is provided with heating resistor, the top lock of cultivateing the dish is connected with end cover, be connected with the function sleeve pipe perpendicularly on end cover's the inner wall, the function sleeve pipe is the cylinder physique, the function sleeve pipe is located the top of cultivateing the test tube and overlaps the outside of locating the cultivation test tube, the function sleeve pipe includes heat conduction pipe box and the net cover of ventilating, the net cover of ventilating of top fixedly connected with of heat conduction pipe box is located end cover's inner wall right side is provided with. The utility model discloses can carry out even accuse temperature heating to cultivateing the test tube, and can concentrate to accomodate the processing to waste water, simple structure, the practicality is strong.
Description
Technical Field
The utility model relates to the field of biotechnology, specifically be a cell culture device that possesses monitoring function.
Background
Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.
The prior cell culture device has the following defects in use: (1) because the thermal resistance is directly adopted for heating, the temperature in the culture cavity is not uniform enough, the temperature of the position close to a heat source is higher, and the temperature is lower on the contrary; (2) meanwhile, a waste water centralized treatment structure is lacked, so that waste water is more time-consuming to clean. For this reason, a corresponding technical scheme needs to be designed to solve the existing technical problems.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
Not enough to prior art, the utility model provides a cell culture device who possesses monitoring function has solved: (1) because the thermal resistor is directly used for heating, the temperature in the culture cavity is not uniform enough, the temperature of the position close to the thermal resistor is higher, and the temperature is lower on the contrary; (2) meanwhile, a waste water centralized treatment structure is lacked, so that the problem that waste water is time-consuming to clean is solved.
(II) technical scheme
In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: the utility model provides a cell culture device that possesses monitoring function, is including cultivateing the dish, the bottom integration of cultivateing the dish is connected with waste water storage disc, it has the cultivation test tube to cultivate longitudinal symmetry and interval equidistance distribution in the inner chamber of dish, the center department of cultivateing the test tube is provided with heating resistor, the top lock of cultivateing the dish is connected with end cover, be connected with the function sleeve pipe perpendicularly on end cover's the inner wall, the function sleeve pipe is the cylinder physique, the function sleeve pipe is located the top of cultivateing the test tube and overlaps the outside of locating the cultivation test tube, the function sleeve pipe includes the heat conduction pipe box and the net cover of ventilating, the net cover is ventilated to the top fixedly connected with of heat conduction pipe cover, is located.
As a further preferred mode of the utility model, the positive left side sealing connection of cultivation dish has the liquid column pipe that leads to.
As a further preferred mode of the utility model, the positive right side sealing connection of cultivateing the dish has the pressure regulating trachea.
As a further preferred mode of the utility model, the inner wall bottom side of cultivateing the dish is provided with the flowing back passageway, flowing back passageway intercommunication waste water storage plate, the outer wall left and right sides symmetry of cultivateing the dish is provided with the strip and buckles the handle.
As a further preferred mode of the utility model, end cover's outer wall top is provided with display instrument, display instrument with liquid level meter and temperature gauge electric connection.
As a further preferred mode of the present invention, the length of the heat conducting pipe sleeve is greater than that of the culture test tube.
(III) advantageous effects
The utility model provides a cell culture device who possesses monitoring function. The method has the following beneficial effects:
(1) this equipment is to the inside injected water of cultivateing the dish, uses heating resistor to heat simultaneously to utilize the function sleeve pipe and the cultivation test tube lock of end cover bottom, utilize the heat conduction pipe box contact hot water of function sleeve pipe bottom to carry out even heat conduction, it is unanimous to reach a plurality of cultivation test tube temperatures of cultivateing the dish inside, has improved the cell culture quality.
(2) This kind of equipment can concentrate waste water and discharge to waste water containing disc at the flowing back passageway of cultivateing the inboard interpolation of dish, cultivates the pressure regulating trachea in the dish outside simultaneously, and the atmospheric pressure of adjustable cultivation dish inner chamber, and liquid level gauge and the temperature gauge monitoring liquid level and temperature on the end cover inner wall can be through the display instrument demonstration, the monitoring of being convenient for.
Drawings
FIG. 1 is a schematic view of the overall external structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the culture tray of the present invention;
fig. 3 is a schematic view of the internal structure of the end cap of the present invention;
fig. 4 is a schematic view of the structure of the functional sleeve of the present invention.
In the figure, 1-culture tray, 2-waste water containing tray, 3-culture test tube, 4-heating resistor, 5-sealing end cover, 6-functional sleeve, 7-heat conducting sleeve, 9-ventilating net sleeve, 10-liquid level meter, 11-temperature meter, 12-liquid column pipe, 13-pressure regulating air pipe, 14-liquid discharge channel, 15-strip-shaped buckle handle and 16-display instrument.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, an embodiment of the present invention provides a technical solution: a cell culture device with a monitoring function comprises a culture tray 1, the bottom end of the culture tray 1 is integrally connected with a wastewater containing tray 2, culture test tubes 3 are symmetrically distributed in the inner cavity of the culture tray 1 up and down at equal intervals, heating resistors 4 are arranged at the center of the culture test tubes 3 and can heat injected moisture, the top of the culture tray 1 is buckled and connected with a sealing end cover 5, a functional sleeve 6 is vertically connected to the inner wall of the sealing end cover 5, the functional sleeve 6 is cylindrical, the functional sleeve 6 is positioned at the top of the culture test tubes 3 and sleeved outside the culture test tubes 3, the functional sleeve 6 comprises a heat conduction sleeve 7 and an air conduction net sleeve 9, the top end of the heat conduction sleeve 7 is fixedly connected with the air conduction net sleeve 9, the heat conduction sleeve 7 can conduct heat, and meanwhile, the ventilation net sleeve 9 can ventilate, and a liquid level gauge 10 and a temperature gauge 11 are arranged on the right side of the inner wall of the sealing end cover 5.
Further, referring to fig. 1, a liquid passing column tube 12 is hermetically connected to the left side of the front surface of the culture tray 1, and thus, water can be injected through the liquid passing column tube 12.
Further, referring to fig. 1, a pressure-regulating air tube 13 is hermetically connected to the right side of the front surface of the culture tray 1, and the pressure-regulating air tube 13 can be used to inject air or extract air to regulate the internal air pressure.
Further, referring to fig. 2, the bottom side of the inner wall of the culture tray 1 is provided with a drainage channel 14, and the left and right sides of the outer wall of the culture tray 1 are symmetrically provided with strip-shaped clasps 15, so that the drainage channel 14 can be used for conveying liquid into the culture tray 1.
Further, referring to fig. 1, a display instrument 16 is arranged on the top of the outer wall of the end cap 5, the display instrument 16 is electrically connected with the liquid level meter 10 and the temperature meter 11, and the liquid level meter 10 and the temperature meter 11 can be used for measuring the liquid level and the temperature, and the display instrument 16 is used for displaying.
Specifically, referring to fig. 1, the length of the heat conducting sleeve 7 is greater than that of the culture tube 3, and the heat conducting sleeve 7 can completely wrap the culture tube 3 due to the design, so that the heat conducting effect is better.
And (4) supplementary notes: the liquid level meter is in a model number of 'E7M', the thermometer is in a model number of 'WSSX-401', and the heating resistor is in a model number of 'ZC-PT 100-01'.
The working principle is as follows: during the use, at first the accessible leads to liquid column pipe 12 to cultivateing 1 inside injected water of dish, 6 covers of function sleeve pipe of 5 bottoms of end cover are established and are being cultivateed test tube 3 outsidely, heat to fixed temperature through using heating resistor 4, hot water evenly conducts the heat to heat conduction pipe box 7, and finally transmit and train test tube 3 for, it is even unanimous that the multiunit is cultivateed test tube 3 temperature to whole having guaranteed, the waste water that has used simultaneously, accessible flowing back passageway 14, transmit to waste water containing disc 2, concentrate and carry out unified processing, it is comparatively convenient.
The utility model comprises 1-culture tray, 2-waste water containing tray, 3-culture test tube, 4-heating resistor, 5-sealing end cover, 6-functional sleeve, 7-heat conducting sleeve, 9-ventilating net cover, 10-liquid level meter, 11-temperature meter, 12-liquid column pipe, 13-pressure regulating air pipe, 14-liquid discharge channel, 15-strip buckle handle, 16-display instrument, all of which are general standard components or components known by technicians in the field, the structure and principle of the utility model can be known by technical manuals or conventional experimental methods, the utility model solves the problems that (1) the heating is directly realized by adopting a thermal resistor, the temperature in the culture cavity is not uniform enough, the temperature of the position close to the thermal resistor is higher, and the temperature is lower on the contrary; (2) lack waste water centralized processing structure simultaneously, lead to clearing up the waste water more problem consuming time, the utility model discloses a combination of each other of above-mentioned part, this equipment is to the inside injected water of cultivateing the dish, use heating resistor to heat simultaneously, and utilize the function sleeve pipe and the cultivation test tube lock of end cover bottom, utilize the heat conduction pipe box contact hot water of function sleeve pipe bottom to carry out even heat conduction, it is unanimous to reach a plurality of cultivation test tube temperatures of cultivateing the dish inside, the cell culture quality has been improved, the flowing back passageway of adding in the inboard of cultivateing the dish, can concentrate waste water and discharge to waste water containing disc, cultivate the pressure regulating trachea in the dish outside simultaneously, the atmospheric pressure of adjustable cultivation dish inner chamber, and liquid level gauge and temperature gauge on the end cover inner wall can monitor liquid level and temperature, show through the.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above, it will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (6)
1. A cell culture device with a monitoring function, characterized in that: comprises a culture tray (1), the bottom end of the culture tray (1) is integrally connected with a waste water containing tray (2), culture test tubes (3) are vertically and symmetrically distributed in the inner cavity of the culture tray (1) at equal intervals, a heating resistor (4) is arranged at the center of the culture test tube (3), the top of the culture tray (1) is buckled and connected with a sealing end cover (5), the inner wall of the sealing end cover (5) is vertically connected with a functional sleeve (6), the functional sleeve (6) is cylindrical, the functional sleeve (6) is positioned at the top of the culture test tube (3) and sleeved outside the culture test tube (3), the functional sleeve (6) comprises a heat-conducting sleeve (7) and a ventilation net sleeve (9), the top end of the heat conduction pipe sleeve (7) is fixedly connected with a ventilation net sleeve (9), and a liquid level meter (10) and a temperature meter (11) are arranged on the right side of the inner wall of the sealing end cover (5).
2. The cell culture apparatus with monitoring function according to claim 1, characterized in that: the left side of the front surface of the culture disc (1) is hermetically connected with a liquid through column tube (12).
3. The cell culture apparatus with monitoring function according to claim 1, characterized in that: the right side of the front surface of the culture disc (1) is hermetically connected with a pressure regulating air pipe (13).
4. The cell culture apparatus with monitoring function according to claim 1, characterized in that: a liquid drainage channel (14) is arranged on the bottom side of the inner wall of the culture tray (1), and strip-shaped buckling handles (15) are symmetrically arranged on the left side and the right side of the outer wall of the culture tray (1).
5. The cell culture apparatus with monitoring function according to claim 1, characterized in that: the liquid level meter is characterized in that a display instrument (16) is arranged on the top of the outer wall of the sealing end cover (5), and the display instrument (16) is electrically connected with the liquid level meter (10) and the temperature meter (11).
6. The cell culture apparatus with monitoring function according to claim 1, characterized in that: the length of the heat conduction pipe sleeve (7) is larger than that of the culture test tube (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020578299.XU CN212357263U (en) | 2020-04-17 | 2020-04-17 | Cell culture device with monitoring function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020578299.XU CN212357263U (en) | 2020-04-17 | 2020-04-17 | Cell culture device with monitoring function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212357263U true CN212357263U (en) | 2021-01-15 |
Family
ID=74132848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020578299.XU Expired - Fee Related CN212357263U (en) | 2020-04-17 | 2020-04-17 | Cell culture device with monitoring function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212357263U (en) |
-
2020
- 2020-04-17 CN CN202020578299.XU patent/CN212357263U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210115 |