CN208038465U - Preparation of Single cell suspensions with CO2Constant temperature incubator - Google Patents
Preparation of Single cell suspensions with CO2Constant temperature incubator Download PDFInfo
- Publication number
- CN208038465U CN208038465U CN201820190289.1U CN201820190289U CN208038465U CN 208038465 U CN208038465 U CN 208038465U CN 201820190289 U CN201820190289 U CN 201820190289U CN 208038465 U CN208038465 U CN 208038465U
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- preparation
- single cell
- cell suspension
- incubation cavity
- cavity
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- 239000006285 cell suspension Substances 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims description 27
- 238000011534 incubation Methods 0.000 claims description 54
- 238000012258 culturing Methods 0.000 claims description 25
- 239000011490 mineral wool Substances 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims 1
- 238000004321 preservation Methods 0.000 abstract description 9
- 229910002092 carbon dioxide Inorganic materials 0.000 description 23
- 238000010438 heat treatment Methods 0.000 description 9
- 230000002070 germicidal effect Effects 0.000 description 4
- 230000000740 bleeding effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides CO for preparing single cell suspension2A constant temperature incubator, relates to CO2The technical field of constant temperature incubator solves the existing CO2The light of incubator inner chamber is poor, is not convenient for observe. And the cultivation frame of inside is mostly fixed, is not convenient for highly adjust and change as required, uses inconvenient technical problem. The device comprises an incubator and CO2Generating device, its characterized in that, be provided with working chamber and heat preservation chamber in the incubator, heat preservation intracavity portion is provided with lighting device, heat preservation intracavity portion is provided with cultivates the frame, cultivate the frame with can dismantle the connection between the heat preservation intracavity wall. The utility model discloses provide lighting device and a plurality of recesses that are used for adjusting culture rack height simultaneously in the thermostated container for light in the thermostated container is good, is convenient for observe. And, cultivate the frame and can inject different recesses as required, realize the adjustable in height, convenient to use.
Description
Technical field
The utility model is related to CO2Constant incubator technical field, the preparation CO of specially a kind of single cell suspension2It is permanent
Warm incubator.
Background technology
CO2Incubator is a kind of device for cultivating cell, and one is formed similar to thin by being simulated in incubator box body
Born of the same parents, the growing environment of tissue in vivo, cultivate cell, tissue.Generally require CO2Have inside incubator constant
Temperature, humidity and gas concentration lwevel.Single cell suspension carbon dioxide incubator during preparation is important a ring
Section, existing CO2The light for cultivating box cavity is poor, is not easy to observe.And internal culturing rack is mostly fixed, is not easy to basis
The height needed is adjusted and replaces, inconvenient to use.Meanwhile existing CO2Incubator uses on the inner wall of incubator
Heating wire is set, and to promote the temperature in incubator, such heating means are easy to cause culture the temperature inside the box unevenness, i.e. inner wall
Portion temperature is higher, and spot temperature is relatively low among incubator, moreover, the speed of electric-heating-wire-heating is slow, it is very not square during use
Just.
Applicants have discovered that at least there is following technical problem in the prior art：
Existing CO2The light for cultivating box cavity is poor, is not easy to observe.And internal culturing rack is mostly fixed, it is inconvenient
It is adjusted and replaces in height as needed, it is inconvenient to use.
Utility model content
The purpose of this utility model is that carrying a kind of preparation CO of single cell suspension2Constant incubator, it is existing to solve
CO2The light for cultivating box cavity is poor, is not easy to observe.And internal culturing rack is mostly fixed, is not easy to as needed
Height is adjusted and replaces, technical problem inconvenient to use.It is preferred in many technical solutions provided by the utility model
Many technique effects (heating rate is fast, easy to operate) elaboration as detailed below that technical solution can be generated.
To achieve the above object, the utility model provides following technical scheme：
The utility model provides a kind of preparation CO of single cell suspension2Constant incubator, including insulating box and CO2Occur
Device is provided with working chamber and incubation cavity in the insulating box, and the incubation cavity is internally provided with lighting device, the incubation cavity
It is internally provided with culturing rack, is detachably connected between the culturing rack and the heat preservation cavity wall.
Preferably, several grooves are provided at left and right sides of the heat preservation cavity wall, the culturing rack is set to two relatively
The groove between, the sliding block with the fit depressions is both provided at left and right sides of the culturing rack.
Preferably, further include warm wind preparation facilities, the warm wind preparation facilities is connected to the inner cavity of incubation cavity.
Preferably, further include air-introduced machine, be set to the top of the warm wind preparation facilities, the air-introduced machine passes through induced duct
It is connected to incubation cavity.
Preferably, the induced duct is located at one end of incubation cavity inner cavity and is provided with air outlet.
Preferably, further include the refrigerating plant for being set to the left and right sides below the rear surface of the incubation cavity inner cavity.
Preferably, it is provided with mineral wool insulating layer in the outer wall inner cavity of the insulating box.
Preferably, the inner wall of the insulating box is provided with mirror face stainless steel plate.
Preferably, further include exhaust pipe, be set to the bottom of the insulating box.
Preferably, it is provided with filter device in the exhaust pipe.
The utility model provides lighting device and several grooves for adjusting culturing rack height simultaneously in insulating box,
So that the light in insulating box is good, convenient for observation.Also, culturing rack can as needed inject in different grooves, realize
It is adjustable in height, it is easy to use.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, other drawings may also be obtained based on these drawings.
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is the utility model vertical cross-sectional.
In figure：1, insulating box；2, thermal insulating door thermally insulated door；3, display panel；4, controller；5, observation window；6, working chamber；7, it keeps the temperature
Chamber；8, warm-air drier；9, air-introduced machine；10, induced duct；11, air outlet；12, refrigerating plant；13,CO2Generating means；14, outlet
Mouthful；15, flow valve；16, temperature sensor；17, humidity sensor；18,CO2Concentration sensor；19, lighting device；20, recessed
Slot；21, culturing rack；22, ultraviolet germicidal lamp；23, exhaust pipe；24, air bleeding valve.
Specific implementation mode
It, below will be to the technology of the utility model to keep the purpose of this utility model, technical solution and advantage clearer
Scheme is described in detail.Obviously, the described embodiments are only a part of the embodiments of the utility model, rather than all
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are not before making creative work
Obtained all other embodiment is put, the range that the utility model is protected is belonged to.
In the description of the present invention, it should be understood that term "center", " longitudinal direction ", " transverse direction ", "front", "rear",
The orientation or positional relationship of the instructions such as "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is based on attached drawing institute
The orientation or positional relationship shown, is merely for convenience of describing the present invention and simplifying the description, rather than indicates or imply and is signified
Device or element must have a particular orientation, with specific azimuth configuration and operation, therefore should not be understood as to this practicality
The limitation of novel protected range.
The utility model provides a kind of preparation CO of single cell suspension2Constant incubator, including insulating box and CO2Hair
Generating apparatus is provided with working chamber and incubation cavity in the insulating box, and the incubation cavity is internally provided with lighting device, the heat preservation
Chamber is internally provided with culturing rack, is detachably connected between the culturing rack and the heat preservation cavity wall.The utility model is in constant temperature
Lighting device and several grooves for adjusting culturing rack height are provided in case simultaneously so that the light in insulating box is good,
Convenient for observation.Also, culturing rack can as needed inject in different grooves, realize being adjusted in height, easy to use.
~2 are please referred to Fig.1, a kind of preparation CO of single cell suspension2Constant incubator includes insulating box 1, described
Insulating box 1 is for accommodating cell or tissue；The front surface of the insulating box 1 is hinged with thermal insulating door thermally insulated door 2, before the thermal insulating door thermally insulated door 2
Surface is disposed with display panel 3, controller 4 and observation window 5 from top to bottom.The display panel 3 is permanent for accurately showing
State in incubator, such as CO2Concentration, humidity, temperature etc..The controller 4 is held for receiving signal and accurately controlling each component
Row relevant operation.The observation window 5 is made of transparent material, can clearly be checked in insulating box 1 for user is convenient
The state of cell or tissue；Working chamber 6 and incubation cavity 7 are provided in 1 inner cavity of the insulating box, and the working chamber 6 is located at
The top of incubation cavity 7.In order to promote the temperature in insulating box 1, it is additionally provided with the inner cavity of warm wind preparation facilities and the incubation cavity 7
Connection.
The situation in incubation cavity 7 is clearly observed for the ease of user, needs to increase illumination, in the incubation cavity 7
Top at left and right sides of chamber is both provided with lighting device 19, and luminous lamp switch is additionally provided on the insulating box 1, and user presses
Lower luminous lamp switch can open lighting device 19, observe the state of the cell or tissue in 7 inner cavity of incubation cavity immediately；Institute
It states and is uniformly arranged below with several grooves 20 at left and right sides of 7 inner arm of incubation cavity, be plugged between groove 20 described in two groups relatively
Culturing rack 21 is detachably connected between the culturing rack 21 and the groove 20.The culturing rack 21 can inject as needed
In different grooves 20, to realize the adjusting in height, connection is reliable, and easy to use；7 inner cavity of the incubation cavity
It is both provided with ultraviolet germicidal lamp 22 at left and right sides of bottom end, ultraviolet radiator switch is provided on insulating box 1.User presses
Ultraviolet radiator switch can open ultraviolet germicidal lamp 22, and sterilization processing is carried out to the inner cavity of incubation cavity 7.
The warm wind preparation facilities is set to the middle position in 1 left side of the insulating box, for generating warm wind.This practicality is new
In type, the warm wind preparation facilities is set as warm-air drier 8.The top of the warm-air drier 8 is connected with air-introduced machine 9 by discharge pipe,
The air-introduced machine extracts warm wind for accelerating.And the air-introduced machine 9 is connected on the left side wall of insulating box 1；The air-introduced machine 9
Top is additionally provided with induced duct 10, and the top that the right end of the induced duct 10 runs through on the left of 1 inner cavity of insulating box extends to working chamber
In 6 inner cavity；The model of warm wind diffusion is had an impact, increased to the culture of cell or tissue in order to reduce the fast warm wind of flow velocity
It encloses, the end of the induced duct 10 is additionally provided with air outlet 11, and the left side on 7 inner cavity top of incubation cavity is run through in the bottom end of air outlet 11
Side.The size of the air outlet 11 is more than the size of the induced duct 10.
8 heating device of warm-air drier has following compared with electric-heating-wire-heating device in the prior art in the utility model
Advantage：First, the speed that warm-air drier 8 generates warm wind is fast, while by the acceleration of air-introduced machine 9 extraction warm wind is continued not
Disconnected enters in incubation cavity 7, greatly improves the heating rate of incubation cavity 7；Second, the warm wind for continuing into incubation cavity 7 is being protected
It is circulated in warm chamber 7, makes to reach in 7 short time of incubation cavity that temperature everywhere is consistent, i.e., temperature is uniform everywhere in incubation cavity 7, has
Conducive to the culture of cell or tissue；The size of third, the air outlet 11 of warm-air drier 8 is larger, be conducive to warm wind timely with compared with
Be covered with entire incubation cavity 7 on a large scale, while the speed of warm wind is slower, to the impact force of cell or tissue in incubation cavity 7 compared with
It is small, the environment of its existence is not influenced；4th, use is safer, and heating wire is set on the inner wall of insulating box 1 in the prior art,
It is also covered with heat-preservation cotton on the heating wire.Such structure has inflammable risk.And warm-air drier 8 can then eliminate this
The risk of sample, use are more safe and reliable.
In order to provide the CO needed for culture to cell or tissue2Environment, the working chamber are provided with CO on the right side of 6 inner cavity2
Generating means 13, for providing sufficient CO for cell or tissue2；The CO2The output end in 13 left side of generating means passes through connection
Pipe is provided with gas outlet 14, and the right side on 7 inner cavity top of incubation cavity is run through in the bottom end of the gas outlet 14.The gas outlet 14
Size is more than the size of connecting tube, the impact force for reducing air-flow to cell or tissue inside incubation cavity 7.Meanwhile even
It takes over and is provided with flow valve 15, for controlling whether to conveying CO in incubation cavity 72Gas, and conveying CO2The stream of gas
Measure size；It is provided with temperature sensor 16 on the left of 7 inner cavity of the incubation cavity, for detecting the real time temperature in incubation cavity 7, and it is anti-
It feeds controller 4, controller 4 controls the work or standby of the warm-air drier 8 after analyzing data；7 inner cavity top of the incubation cavity
Right side be provided with humidity sensor 17 and CO2Concentration sensor 18, for detecting real-time humidity and CO in incubation cavity 72It is dense
Degree, and by the data feedback of detection to controller 4, controller 4 controls CO after carrying out Data Analysis Services2Generating means 13 works
Or it is standby.
When temperature sensor 16 detects that the temperature in incubation cavity 7 is higher than setting value, need to carry out cooling processing, so
The left and right sides below 7 inner cavity rear surface of the incubation cavity is both provided with refrigerating plant 12.Temperature sensor 16 is by temperature height
Controller 4 is fed back in the signal of setting value, execution order is transferred to refrigerating plant 12 by controller 4 makes it start to freeze.When
When temperature sensor 16 detects that the temperature in incubation cavity 7 is in range of set value, controller 4, which controls refrigerating plant 12, to be stopped
Work, it is ensured that temperature is constant in incubation cavity 7.
In order to which the exhaust gas in 7 inner cavity of incubation cavity to be discharged, the middle position of 7 inner cavity bottom end of the incubation cavity is provided with exhaust
Pipe 23 is provided with air bleeding valve 24 in the inner cavity of the exhaust pipe 23.
The controller 4 respectively with display panel 3, warm-air drier 8, air-introduced machine 9, refrigerating plant 12, CO2Generating means 13,
Flow valve 15, temperature sensor 16, humidity sensor 17, CO2Concentration sensor 18, lighting device 19 and ultraviolet germicidal lamp 22
It is electrically connected.
Wherein, it is provided with mineral wool insulating layer in the outer wall inner cavity of the insulating box 1, effectively improves its heat insulation effect；It is described
The inner wall of insulating box 1 is provided with mirror face stainless steel plate, effectively improves its Corrosion Protection；The bottom end of the exhaust pipe 23 is connected with
Filter device reduces pollution of the gas discharge to environment.The left and right sides of the culturing rack 21 is both provided with to match with groove 20
The sliding block of conjunction so that culturing rack 21 is flexibly connected with groove 20, convenient for the adjustment of progress 21 spacing of culturing rack as needed and more
It changes, the sliding block can be pulley.
The operation principle of the utility model is as follows：
In use, the cell being collected into is placed on culturing rack 21, closes on thermal insulating door thermally insulated door 2, be arranged in controller 4
Temperature, humidity and CO2The preset value of concentration；Open CO2Generating means 13, CO2Gas is entered by connecting tube to gas outlet 14
In the inner cavity of incubation cavity 7；CO2Concentration sensor 18 detects the CO of its inner cavity2Concentration, when concentration be more than or less than preset value when,
Controller 4 controls to adjust the adjusting that valve 15 carries out flow；Warm-air drier 8 is opened, by air-introduced machine 9 so that warm wind passes through air outlet 11
Into in the inner cavity of incubation cavity 7, heat up to the inner cavity of incubation cavity 7.When temperature is more than preset value, humidity is less than preset value
When, warm-air drier 8 is closed, refrigerating plant 12 is opened, cools down to the inner cavity of incubation cavity 7.When humidity is more than preset value, temperature is small
When preset value, refrigerating plant 12 is closed, the power of warm-air drier 8 is improved, to reach the constant of 7 inner cavity temperature of incubation cavity；It is logical
Setting lighting device 19 is crossed, the light of 7 inner cavity of incubation cavity is improved；By the way that observation window 5 and display panel 3 is arranged, convenient for observing it
Inner cavity and humiture and CO2The variation of concentration；By the way that exhaust pipe 23 and air bleeding valve 24 is arranged, convenient for excluding gas；In exhaust pipe
23 end set filter device reduces pollution of the gas discharge to environment.
Above description is only a specific implementation of the present invention, but the scope of protection of the utility model is not limited to
In this, any one skilled in the art within the technical scope disclosed by the utility model, can readily occur in variation
Or replace, it should be covered within the scope of the utility model.Therefore, the scope of protection of the utility model should be with the power
Subject to the protection domain that profit requires.
Claims (10)
1. a kind of preparation CO of single cell suspension2Constant incubator, including insulating box (1) and CO2Generating means (13), it is special
Sign is, working chamber (6) and incubation cavity (7) are provided in the insulating box (1), and the incubation cavity (7) is internally provided with illumination
Device (19), the incubation cavity (7) are internally provided with culturing rack (21), the culturing rack (21) and the incubation cavity (7) inner wall
Between be detachably connected.
2. the preparation CO of single cell suspension according to claim 12Constant incubator, which is characterized in that the incubation cavity
Several grooves (20) are provided at left and right sides of inner wall, the culturing rack is set between two opposite grooves (20), institute
It states and is both provided at left and right sides of culturing rack (21) and the groove (20) matched sliding block.
3. the preparation CO of single cell suspension according to claim 22Constant incubator, which is characterized in that further include warm wind
Preparation facilities, the warm wind preparation facilities are connected to the inner cavity of incubation cavity (7).
4. the preparation CO of single cell suspension according to claim 32Constant incubator, which is characterized in that further include air inducing
Machine (9), is set to the top of the warm wind preparation facilities, and the air-introduced machine (9) is connected by induced duct (10) and incubation cavity (7)
It is logical.
5. the preparation CO of single cell suspension according to claim 42Constant incubator, which is characterized in that the induced duct
(10) one end for being located at incubation cavity (7) inner cavity is provided with air outlet (11).
6. the preparation CO of single cell suspension according to claim 52Constant incubator, which is characterized in that further include setting
The refrigerating plant (12) of the left and right sides below the rear surface of the incubation cavity (7) inner cavity.
7. the preparation CO of single cell suspension according to claim 62Constant incubator, which is characterized in that the insulating box
(1) mineral wool insulating layer is provided in outer wall inner cavity.
8. the preparation CO of single cell suspension according to claim 72Constant incubator, which is characterized in that the insulating box
(1) inner wall is provided with mirror face stainless steel plate.
9. the preparation CO of single cell suspension according to claim 82Constant incubator, which is characterized in that further include exhaust
It manages (23), is set to the bottom of the insulating box (1).
10. the preparation CO of single cell suspension according to claim 92Constant incubator, which is characterized in that the exhaust
It is provided with filter device in pipe (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820190289.1U CN208038465U (en) | 2018-02-01 | 2018-02-01 | Preparation of Single cell suspensions with CO2Constant temperature incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820190289.1U CN208038465U (en) | 2018-02-01 | 2018-02-01 | Preparation of Single cell suspensions with CO2Constant temperature incubator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208038465U true CN208038465U (en) | 2018-11-02 |
Family
ID=63951761
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820190289.1U Active CN208038465U (en) | 2018-02-01 | 2018-02-01 | Preparation of Single cell suspensions with CO2Constant temperature incubator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208038465U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111154625A (en) * | 2020-03-09 | 2020-05-15 | 河南赛诺优农科技有限公司 | Culture box |
-
2018
- 2018-02-01 CN CN201820190289.1U patent/CN208038465U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111154625A (en) * | 2020-03-09 | 2020-05-15 | 河南赛诺优农科技有限公司 | Culture box |
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