CN109022239A - A kind of device and method with mesona concentrate fermentative lactobacillus filial piety element - Google Patents

A kind of device and method with mesona concentrate fermentative lactobacillus filial piety element Download PDF

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CN109022239A
CN109022239A CN201810730986.6A CN201810730986A CN109022239A CN 109022239 A CN109022239 A CN 109022239A CN 201810730986 A CN201810730986 A CN 201810730986A CN 109022239 A CN109022239 A CN 109022239A
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mesona
concentrate
filtrate
plc controller
filial piety
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曹华斌
焦广飞
林永英
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LONGYAN JIALIN BIOLOGICAL TECHNOLOGY Co Ltd
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LONGYAN JIALIN BIOLOGICAL TECHNOLOGY Co Ltd
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/02Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins

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Abstract

The invention discloses a kind of device and methods with mesona concentrate fermentative lactobacillus filial piety element, preparation method includes the following steps: the preparation of mesona concentrate, the production of lactic acid bacteria culturing medium, inoculation and fermentation, when the present invention uses, the displacement signal of servo motor is acquired by the displacement sensor of installation, and the input terminal of PLC controller is passed to through its output end, after PLC controller analysis processing, if displacement signal is less than PLC controller pre- defeated permissible value, then work normally, if if when displacement signal is greater than PLC controller pre- defeated permissible value, then the output end of PLC controller can issue instructions to power switch, power switch is controlled to close, prevent motor shaft abrasion aggravation, the viewfinder of setting is convenient for the observation to internal fermentation situation, the protective shell of setting, glass tube and hole slot, internal fermentation liquid into Enter glass tube, observation can be carried out by hole slot and observed convenient for liquid level, production method is easy to operate, and product quality is high.

Description

A kind of device and method with mesona concentrate fermentative lactobacillus filial piety element
Technical field
The present invention relates to lactic acid bacteria filial piety element production field, specially a kind of dress with mesona concentrate fermentative lactobacillus filial piety element It sets and method.
Background technique
Lactic acid bacteria is the general name for referring to the bacterium of glucose fermentation or lactose generation lactic acid.Due to various in modern society The influence of poor eating habits factor, the flora in human body intestinal canal is out of proportion, and lactic acid bacteria product can make in human body intestinal canal Lactic acid bacterium number increased significantly within than the short period, reduce the quantity of pernicious bacteria, finally make the strain of human body intestinal canal Group reaches balance.Meanwhile the lactose taken in outside human body is utilized by lactic acid bacteria in vivo, is fermented, and lactic acid, a side are generated Face strengthens the acidity in enteron aisle, and on the other hand, the bacteriocin in lactic acid fermented product can be reduced some spoilage organisms and low temperature Bacterium plays the balance of protection human normal physiological function and intestinal flora, and the device and method of existing lactic acid bacteria filial piety element There are some defects.
So how to design a kind of device and method with mesona concentrate fermentative lactobacillus filial piety element, it is current to become us It solves the problems, such as.
Summary of the invention
The present invention provides a kind of device and method with mesona concentrate fermentative lactobacillus filial piety element, effectively to solve above-mentioned back The problems in scape technology.
To achieve the above object, the invention provides the following technical scheme: a kind of mesona concentrate fermentative lactobacillus filial piety element Device, including device noumenon, shaft coupling, servo motor, PLC controller, displacement sensor, viewfinder, feed inlet, sampling Mouth, cooling water outlet, thermometer mouth, discharge gate, fixing bolt, protective shell, thermocouple port, electrode ports, cooling water inlet, stirring The top of axis, bubbler, agitating paddle, glass tube and hole slot, described device ontology is equipped with servo motor by shaft coupling, described The side of servo motor is equipped with PLC controller, and the side of the shaft coupling is equipped with displacement sensor, described device ontology top The two sides at end are respectively arranged with viewfinder and feed inlet, are provided with sample tap below the feed inlet, under the sample tap Side is provided with cooling water outlet, and the side in the middle part of described device ontology is provided with thermometer mouth, the lower section setting of the viewfinder There is discharge gate, the lower section of the discharge gate is equipped with protective shell by fixing bolt, is provided with thermoelectricity below the protective shell Even mouth, described device body bottom portion setting side are provided with electrode ports, and the side of described device ontology bottom end is provided with cooling water Import is plugged with agitating shaft in the middle part of described device ontology, and the top of the agitating shaft is connect with shaft coupling, the agitating shaft Top is welded with bubbler, and uniform welding has agitating paddle on the agitating shaft, and the inside of the protective shell is equipped with glass tube, institute It states and offers hole slot on protective shell, the output end of institute's displacement sensors is electrically connected the input terminal of PLC controller, the PLC The output end of controller is electrically connected power switch.
A method of with mesona concentrate fermentative lactobacillus filial piety element, characterized by the following steps:
One, the preparation of mesona concentrate
Mesona 500g is taken, 10 times of 95% ethyl alcohol is added after crushing, is impregnated for 24 hours at 60 DEG C, soaking process will be stirred often, nylon cloth Filtering, obtains filtrate A, and filter residue adds 8 times of 85% ethyl alcohol of amount, obtains liquor B according to preceding method, filter residue again in enter 6 times of 75% ethyl alcohol of amount, according to preceding Method obtains liquor C, and merging filtrate A, B, C are saved overnight in 4~8 DEG C, and after filtrate is using filter, vacuum distillation recycling ethyl alcohol will be returned Liquid after receiving ethyl alcohol pours into mild fire in enamel basin and heats, and partial moisture is evaporated, until being concentrated into 1000ml, in above-mentioned concentration 0.3% active carbon is added in liquid, boils 10min, decolourizes, filtering, filtrate answers clear, and 0.2% Tween 80 is added after cooling, stirs It is even after filtering, in filtrate be added 1%~2% benzyl alcohol, mix, then use 10% sodium hydroxide tune pH6.5 or so, 4~8 DEG C save Overnight, it after coarse filtration, is filtered with 94 flint glass F degerming funnels, or by degerming filter plate, such as not up to clarity should refilter one Secondary, the mesona concentrate after degerming is fitted into ampoule, is sealed, and ampoule is qualified mesona concentration after steam sterilizing again Liquid;
Two, the production of lactic acid bacteria culturing medium
By skimmed milk and water with the ratio of 1:7W/V, at the same be added 6% sucrose, be sufficiently mixed, sterilize 10 in 80~85 DEG C~ 15min is cooled to 35~40 DEG C, the culture substrate as production lactic acid bacteria;
Three, it is inoculated with
Using fresh yogurt and mesona concentrate as leavening, it is respectively connected to be lactic acid in the above culture substrate with 2% inoculum concentration Fermentation liquid shakes up after inoculation, then lactic fermentation liquid is mounted in the container sterilized;
Four, it ferments
Lactic fermentation liquid after inoculation is poured into the device of mesona concentrate fermentative lactobacillus filial piety element, setting temperature is 40~42 When DEG C 3~4h of culture, when fermentation, pays attention to observing, and stops fermentation after there is curdled milk, then again takes out lactic fermentation liquid, be transferred to 4 It is refrigerated more than for 24 hours in~5 DEG C of refrigerators, through this after-ripening stage, reaches mild acidity, pH4~4.5, grumeleuse even compact, no whey It is precipitated, no steam bubble completes fermentation.
According to the above technical scheme, the viewfinder is a kind of component of high temperature high voltage resistant material, and outside passes through hinge Protective cover is installed.
According to the above technical scheme, protective cover is installed, and servo motor is electrically connected control on the outside of the servo motor System switch.
According to the above technical scheme, the production method of the mesona concentrate is made after can also being extracted by mesona mycelium At mesona concentrate, method are as follows: after mycelia is dried or dried, crush, be soaked in water, boil 2h, obtain filtrate, boil 3 altogether Secondary, merging filtrate is condensed into thick, lets cool, and adding ethyl alcohol to solution alcohol content is 75%, places 48h or more, proposes filter, recycling second Alcohol obtains medical fluid, and 10% sodium hydroxide is added in medical fluid, adjusts pH to 8, stands overnight, and proposes filter, removes precipitating, filtrate dilute sulfuric acid PH to 6 is adjusted, suction filtration goes to precipitate, and adding ethyl alcohol to solution alcohol content is 85%, places 48h or more, and suction filtration goes to precipitate, and filtrate recycles wine Essence, is concentrated into alcohol-free, and 0.5% active carbon is added, heats 30min, takes filtrate spare after suction filtration, separately distilled water is taken to be heated to 60 DEG C, 1% benzyl alcohol is added, is poured into above-mentioned spare filtrate, stirs evenly after dissolution, distilled water is added to enough, it is vertical with No. 3 after coarse filtration Molten funnel refined filtration, encapsulating sterilize in ampoule to get mesona concentrate.
According to the above technical scheme, the glass tube is a kind of component of transparent high pressure resistant material, and its bottom end and device The inside of ontology is connected, and top is provided with ventilation mouth.
According to the above technical scheme, the hole slot is internally provided with transparency protected shell, and the transparency protected shell and hole The junction of slot is provided with rubber seal.
Compared with prior art, beneficial effects of the present invention: in use, acquiring servo electricity by the displacement sensor of installation The displacement signal of machine, and the input terminal of PLC controller is passed to through its output end, after PLC controller analysis processing, if displacement When signal is less than PLC controller pre- defeated permissible value, then work normally, if PLC controller is pre- defeated to be permitted if displacement signal is greater than When being worth perhaps, then the output end of PLC controller can issue instructions to power switch, and control power switch is closed, and prevent motor shaft from grinding Damage aggravation, the viewfinder of setting are convenient for the observation to internal fermentation situation, protective shell, glass tube and the hole slot of setting, inside hair Zymotic fluid enters glass tube, can carry out observation by hole slot and observe convenient for liquid level, and production method is easy to operate, and product quality is high.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention It applies example to be used to explain the present invention together, not be construed as limiting the invention.
In the accompanying drawings:
Fig. 1 is overall structure diagram of the invention;
Fig. 2 is cross-sectional view of the invention;
Fig. 3 is protective shell structural schematic diagram of the present invention;
Fig. 4 is the structural schematic diagram of A in Fig. 1 of the present invention;
Fig. 5 is production process block diagram of the invention;
Figure label: 1, device noumenon;2, shaft coupling;3, servo motor;4, PLC controller;5, displacement sensor;6, it visits Mirror;7, feed inlet;8, sample tap;9, cooling water outlet;10, thermometer mouth;11, discharge gate;12, fixing bolt;13, it protects Shell;14, thermocouple port;15, electrode ports;16, cooling water inlet;17, agitating shaft;18, bubbler;19, agitating paddle;20, glass Pipe;21, hole slot.
Specific embodiment
Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings, it should be understood that preferred reality described herein Apply example only for the purpose of illustrating and explaining the present invention and is not intended to limit the present invention.
Embodiment: as shown in Figs. 1-5, the present invention provides a kind of device with mesona concentrate fermentative lactobacillus filial piety element, packet Include device noumenon 1, shaft coupling 2, servo motor 3, PLC controller 4, displacement sensor 5, viewfinder 6, feed inlet 7, sample tap 8, Cooling water outlet 9, thermometer mouth 10, discharge gate 11, fixing bolt 12, protective shell 13, thermocouple port 14, electrode ports 15, cooling Water inlet 16, agitating shaft 17, bubbler 18, agitating paddle 19, glass tube 20 and hole slot 21, the top of device noumenon 1 pass through shaft coupling Device 2 is equipped with servo motor 3, and the side of servo motor 3 is equipped with PLC controller 4, and the side of shaft coupling is equipped with displacement sensing Device 5, the two sides on 1 top of device noumenon are respectively arranged with viewfinder 6 and feed inlet 7, and the lower section of feed inlet 7 is provided with sample tap 8, The lower section of sample tap 8 is provided with cooling water outlet 9, and the side at 1 middle part of device noumenon is provided with thermometer mouth 10, viewfinder 6 Lower section is provided with discharge gate 11, and the lower section of discharge gate 11 is equipped with protective shell 13, the lower section of protective shell 13 by fixing bolt 12 It is provided with thermocouple port 14,1 bottom of device noumenon setting side is provided with electrode ports 15, the side setting of 1 bottom end of device noumenon There is cooling water inlet 16, the middle part of device noumenon 1 is plugged with agitating shaft 17, and the top of agitating shaft 17 is connect with shaft coupling 2, stirring The top of axis 17 is welded with bubbler 18, and uniform welding has agitating paddle 19 on agitating shaft 17, and the inside of protective shell 13 is equipped with glass Glass pipe 20 offers hole slot 21 on protective shell 13, and the output end of displacement sensor 5 is electrically connected the input terminal of PLC controller 4, The output end of PLC controller 4 is electrically connected power switch.
A method of with mesona concentrate fermentative lactobacillus filial piety element, characterized by the following steps:
One, the preparation of mesona concentrate
Mesona 500g is taken, 10 times of 95% ethyl alcohol is added after crushing, is impregnated for 24 hours at 60 DEG C, soaking process will be stirred often, nylon cloth Filtering, obtains filtrate A, and filter residue adds 8 times of 85% ethyl alcohol of amount, obtains liquor B according to preceding method, filter residue again in enter 6 times of 75% ethyl alcohol of amount, according to preceding Method obtains liquor C, and merging filtrate A, B, C are saved overnight in 4~8 DEG C, and after filtrate is using filter, vacuum distillation recycling ethyl alcohol will be returned Liquid after receiving ethyl alcohol pours into mild fire in enamel basin and heats, and partial moisture is evaporated, until being concentrated into 1000ml, in above-mentioned concentration 0.3% active carbon is added in liquid, boils 10min, decolourizes, filtering, filtrate answers clear, and 0.2% Tween 80 is added after cooling, stirs It is even after filtering, in filtrate be added 1%~2% benzyl alcohol, mix, then use 10% sodium hydroxide tune pH6.5 or so, 4~8 DEG C save Overnight, it after coarse filtration, is filtered with 94 flint glass F degerming funnels, or by degerming filter plate, such as not up to clarity should refilter one Secondary, the mesona concentrate after degerming is fitted into ampoule, is sealed, and ampoule is qualified mesona concentration after steam sterilizing again Liquid;
Two, the production of lactic acid bacteria culturing medium
By skimmed milk and water with the ratio of 1:7W/V, at the same be added 6% sucrose, be sufficiently mixed, sterilize 10 in 80~85 DEG C~ 15min is cooled to 35~40 DEG C, the culture substrate as production lactic acid bacteria;
Three, it is inoculated with
Using fresh yogurt and mesona concentrate as leavening, it is respectively connected to be lactic acid in the above culture substrate with 2% inoculum concentration Fermentation liquid shakes up after inoculation, then lactic fermentation liquid is mounted in the container sterilized;
Four, it ferments
Lactic fermentation liquid after inoculation is poured into the device of mesona concentrate fermentative lactobacillus filial piety element, setting temperature is 40~42 When DEG C 3~4h of culture, when fermentation, pays attention to observing, and stops fermentation after there is curdled milk, then again takes out lactic fermentation liquid, be transferred to 4 It is refrigerated more than for 24 hours in~5 DEG C of refrigerators, through this after-ripening stage, reaches mild acidity, pH4~4.5, grumeleuse even compact, no whey It is precipitated, no steam bubble completes fermentation.
According to the above technical scheme, viewfinder 6 is a kind of component of high temperature high voltage resistant material, and outside is installed by hinge There is protective cover.
According to the above technical scheme, the outside of servo motor 3 is equipped with protective cover, and servo motor 3 is electrically connected control Switch.
According to the above technical scheme, celestial being is made after can also extracting by mesona mycelium in the production method of mesona concentrate Careless concentrate, method are as follows: after mycelia is dried or dried, crush, be soaked in water, boil 2h, obtain filtrate, boil altogether 3 times, close And filtrate, it is condensed into thick, lets cool, adding ethyl alcohol to solution alcohol content is 75%, places 48h or more, proposes filter, and recycling ethyl alcohol obtains 10% sodium hydroxide is added in medical fluid for medical fluid, adjusts pH to 8, stands overnight, and proposes filter, removes and precipitates, and filtrate is with dilute sulfuric acid tune pH To 6, suction filtration goes to precipitate, and adding ethyl alcohol to solution alcohol content is 85%, places 48h or more, and suction filtration goes to precipitate, and filtrate recovered alcohol is dense It is reduced to alcohol-free, 0.5% active carbon is added, heats 30min, takes filtrate spare after suction filtration, separately takes distilled water to be heated to 60 DEG C, add Enter 1% benzyl alcohol, be poured into above-mentioned spare filtrate, stir evenly after dissolution, distilled water is added to enough, with No. 3 sintered filter funnels after coarse filtration Refined filtration, encapsulating sterilize in ampoule to get mesona concentrate.
According to the above technical scheme, glass tube 20 is a kind of component of transparent high pressure resistant material, and its bottom end and device sheet The inside of body 1 is connected, and top is provided with ventilation mouth.
According to the above technical scheme, hole slot 21 is internally provided with transparency protected shell, and transparency protected shell and hole slot 21 Junction is provided with rubber seal.
Based on above-mentioned, it is an advantage of the current invention that in use, the displacement sensor 5 by installation acquires servo motor 3 Displacement signal, and the input terminal of PLC controller 4 is passed to through its output end, after 4 analysis of PLC controller processing, if displacement letter When number being less than the pre- defeated permissible value of PLC controller 4, then work normally, if PLC controller 4 is pre- defeated to be permitted if displacement signal is greater than When being worth perhaps, then the output end of PLC controller 4 can issue instructions to power switch, and control power switch is closed, and prevent motor shaft from grinding Damage aggravation, the viewfinder 6 of setting are convenient for the observation to internal fermentation situation, protective shell 13, glass tube 20 and the hole slot 21 of setting, Internal fermentation liquid enters glass tube 20, can carry out observation by hole slot 21 and observe convenient for liquid level, production method is easy to operate, product matter Amount is high.
Finally, it should be noted that being not intended to restrict the invention the foregoing is merely preferred embodiment of the invention, to the greatest extent Present invention has been described in detail with reference to the aforementioned embodiments for pipe, still can be with for the technology patient of this field It modifies the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.It is all Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in guarantor of the invention Within the scope of shield.

Claims (7)

1. a kind of device with mesona concentrate fermentative lactobacillus filial piety element, including device noumenon (1), shaft coupling (2), servo motor (3), PLC controller (4), displacement sensor (5), viewfinder (6), feed inlet (7), sample tap (8), cooling water outlet (9), temperature Degree meter mouth (10), discharge gate (11), fixing bolt (12), protective shell (13), thermocouple port (14), electrode ports (15), cooling water Import (16), agitating shaft (17), bubbler (18), agitating paddle (19), glass tube (20) and hole slot (21), it is characterised in that: institute The top for stating device noumenon (1) is equipped with servo motor (3) by shaft coupling (2), and the side of the servo motor (3) is equipped with PLC controller (4), the side of the shaft coupling are equipped with displacement sensor (5), the two sides point on described device ontology (1) top It is not provided with viewfinder (6) and feed inlet (7), is provided with sample tap (8) below the feed inlet (7), the sample tap (8) Lower section be provided with cooling water outlet (9), the side in the middle part of described device ontology (1) is provided with thermometer mouth (10), the spy It is provided with below visor (6) discharge gate (11), the lower section of the discharge gate (11) is equipped with protection by fixing bolt (12) Shell (13) is provided with thermocouple port (14) below the protective shell (13), and described device ontology (1) bottom is arranged side and is arranged Have electrode ports (15), the side of described device ontology (1) bottom end is provided with cooling water inlet (16), described device ontology (1) Middle part is plugged with agitating shaft (17), and the top of the agitating shaft (17) is connect with shaft coupling (2), the top of the agitating shaft (17) It is welded with bubbler (18), there is agitating paddle (19) uniform welding on the agitating shaft (17), the inside peace of the protective shell (13) Equipped with glass tube (20), offered hole slot (21) on the protective shell (13), the output end of institute's displacement sensors (5) electrically connects The input terminal of PLC controller (4) is connect, the output end of the PLC controller (4) is electrically connected power switch.
2. a kind of method with mesona concentrate fermentative lactobacillus filial piety element, characterized by the following steps:
One, the preparation of mesona concentrate
Mesona 500g is taken, 10 times of 95% ethyl alcohol is added after crushing, is impregnated for 24 hours at 60 DEG C, soaking process will be stirred often, nylon cloth Filtering, obtains filtrate A, and filter residue adds 8 times of 85% ethyl alcohol of amount, obtains liquor B according to preceding method, filter residue again in enter 6 times of 75% ethyl alcohol of amount, according to preceding Method obtains liquor C, and merging filtrate A, B, C are saved overnight in 4~8 DEG C, and after filtrate is using filter, vacuum distillation recycling ethyl alcohol will be returned Liquid after receiving ethyl alcohol pours into mild fire in enamel basin and heats, and partial moisture is evaporated, until being concentrated into 1000ml, in above-mentioned concentration 0.3% active carbon is added in liquid, boils 10min, decolourizes, filtering, filtrate answers clear, and 0.2% Tween 80 is added after cooling, stirs It is even after filtering, in filtrate be added 1%~2% benzyl alcohol, mix, then use 10% sodium hydroxide tune pH6.5 or so, 4~8 DEG C save Overnight, it after coarse filtration, is filtered with 94 flint glass F degerming funnels, or by degerming filter plate, such as not up to clarity should refilter one Secondary, the mesona concentrate after degerming is fitted into ampoule, is sealed, and ampoule is qualified mesona concentration after steam sterilizing again Liquid;
Two, the production of lactic acid bacteria culturing medium
By skimmed milk and water with the ratio of 1:7W/V, at the same be added 6% sucrose, be sufficiently mixed, sterilize 10 in 80~85 DEG C~ 15min is cooled to 35~40 DEG C, the culture substrate as production lactic acid bacteria;
Three, it is inoculated with
Using fresh yogurt and mesona concentrate as leavening, it is respectively connected to be lactic acid in the above culture substrate with 2% inoculum concentration Fermentation liquid shakes up after inoculation, then lactic fermentation liquid is mounted in the container sterilized;
Four, it ferments
Lactic fermentation liquid after inoculation is poured into the device of mesona concentrate fermentative lactobacillus filial piety element, setting temperature is 40~42 When DEG C 3~4h of culture, when fermentation, pays attention to observing, and stops fermentation after there is curdled milk, then again takes out lactic fermentation liquid, be transferred to 4 It is refrigerated more than for 24 hours in~5 DEG C of refrigerators, through this after-ripening stage, reaches mild acidity pH4~4.5, grumeleuse even compact, no whey It is precipitated, no steam bubble completes fermentation.
3. a kind of device with mesona concentrate fermentative lactobacillus filial piety element according to claim 1, it is characterised in that: described Viewfinder (6) is a kind of component of high temperature high voltage resistant material, and outside is equipped with protective cover by hinge.
4. a kind of device with mesona concentrate fermentative lactobacillus filial piety element according to claim 1, it is characterised in that: described Protective cover is installed, and servo motor (3) is electrically connected control switch on the outside of servo motor (3).
5. a kind of method with mesona concentrate fermentative lactobacillus filial piety element according to claim 2, it is characterised in that: described Mesona concentrate, method is made after can also extracting by mesona mycelium in the production method of mesona concentrate are as follows: by mycelia After drying or drying, crushes, be soaked in water, boil 2h, obtain filtrate, boil altogether 3 times, merging filtrate, be condensed into thick, put Cold, adding ethyl alcohol to solution alcohol content is 75%, places 48h or more, proposes filter, recycles ethyl alcohol, obtains medical fluid, 10% hydrogen-oxygen is added in medical fluid Change sodium, adjust pH to 8, stand overnight, propose filter, remove precipitating, filtrate goes to precipitate with dilute sulfuric acid tune pH to 6, suction filtration, adds ethyl alcohol extremely Solution alcohol content is 85%, places 48h or more, and suction filtration goes to precipitate, filtrate recovered alcohol, is concentrated into alcohol-free, 0.5% activity of addition Charcoal heats 30min, takes filtrate spare after suction filtration, distilled water is separately taken to be heated to 60 DEG C, 1% benzyl alcohol is added, is poured into after dissolution It states in spare filtrate, stirs evenly, distilled water is added to enough, No. 3 sintered filter funnel refined filtrations are used after coarse filtration, encapsulating sterilizes in ampoule, Up to mesona concentrate.
6. a kind of method with mesona concentrate fermentative lactobacillus filial piety element according to claim 1, it is characterised in that: described Glass tube (20) is a kind of component of transparent high pressure resistant material, and its bottom end is connected with the inside of device noumenon (1), and top is set It is equipped with ventilation mouth.
7. a kind of method with mesona concentrate fermentative lactobacillus filial piety element according to claim 2, it is characterised in that: described Hole slot (21) is internally provided with transparency protected shell, and to be provided with rubber close for the junction of the transparency protected shell and hole slot (21) Seal.
CN201810730986.6A 2018-07-05 2018-07-05 A kind of device and method with mesona concentrate fermentative lactobacillus filial piety element Withdrawn CN109022239A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110639034A (en) * 2019-09-19 2020-01-03 泉州台商投资区春凯机械科技有限公司 Solid state fermentation culture medium sterilization equipment
CN113519619A (en) * 2021-07-23 2021-10-22 广州市泰伯生物科技有限公司 Method for preparing lactic acid bacteria fermentation concentrated solution and corresponding system thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110639034A (en) * 2019-09-19 2020-01-03 泉州台商投资区春凯机械科技有限公司 Solid state fermentation culture medium sterilization equipment
CN110639034B (en) * 2019-09-19 2021-10-08 北京炳洋科技有限公司 Solid state fermentation culture medium sterilization equipment
CN113519619A (en) * 2021-07-23 2021-10-22 广州市泰伯生物科技有限公司 Method for preparing lactic acid bacteria fermentation concentrated solution and corresponding system thereof
CN113519619B (en) * 2021-07-23 2023-09-12 广州市泰伯生物科技有限公司 Method for preparing lactobacillus fermentation concentrated solution and corresponding system

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