CN102234606A - Simple and convenient microbial cultivation equipment capable of sterilizing and cultivation method thereof - Google Patents
Simple and convenient microbial cultivation equipment capable of sterilizing and cultivation method thereof Download PDFInfo
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- CN102234606A CN102234606A CN2010101700154A CN201010170015A CN102234606A CN 102234606 A CN102234606 A CN 102234606A CN 2010101700154 A CN2010101700154 A CN 2010101700154A CN 201010170015 A CN201010170015 A CN 201010170015A CN 102234606 A CN102234606 A CN 102234606A
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Abstract
The invention relates to a simple and convenient microorganism culture equipment capable of sterilizing and a culture method thereof, wherein the equipment comprises a culture tank, and the culture tank is provided with an exhaust port; a stirring device is arranged on the side of the culture tank in a penetrating way; a breather pipe is arranged at the side of the culture tank in a penetrating way, and gas is introduced into the breather pipe and is exhausted through an exhaust port; an air filter is positioned in the culture tank and connected with the vent pipe; a heating device is arranged at the bottom end of the culture tank; a cooling device is arranged in the culture tank. The invention has simple structure and low cost, and can be used by a user for self-culturing microorganisms in a low-cost mode, and the air filter arranged in the culture tank can kill bacteria, and the vent pipe is introduced with gas and then is exhausted from the exhaust port, so that the air in the culture tank can be continuously exhausted, and the cultured microorganisms can be prevented from being polluted by the bacteria entering the culture tank from the outside.
Description
Technical field
The invention relates to a kind of microbial cultivation equipment, be meant a kind of easy microbial cultivation equipment and cultural method thereof of sterilizing especially.
Background technology
In recent years, the use of microorganism significantly improves human life quality, the use range of microorganism is extensive, generally be used in cause of disease, insect, control of weeds more or bring out crop, or by the effective constituent of microorganism via the made product of prescription, generally separating obtained by nature, the only also artificial strain improvement of warp again is mutagenesis, eliminates and select or the gene transformation as the people.With the most nontoxicitys of product of microorganism making, and in environment, can be decomposed by biology.Microbe species is numerous, comprises bacterium, fungi, algae, protozoon or virus.Its mechanism of action can via the production (as the Su Li bacterium, Bacillus thuringiensis) of toxin, invade parasitic (as white stiff bacterium, Beauveria), pathogenicity bo (as baculovirus) and compete (cross protection that is used for plant as virus).
Microorganism can do effectively to eliminate at specific insect, to people or other animals and plants nontoxicity, again because of its tool Biodegradable, thus can the residual contamination environment, for a long time promptly the commercialization mass production be used for the control of insect pest.Yet general microorganism culturing is difficult for, must could culturing micro-organisms with specific device or equipment, and specific device or equipment manufacturing cost are too high, and non-its a large amount of use of cost can be born.Though the research of microorganism culturing is a lot of in diplomatic record, technical ripe, mostly be breadboard achievement in research, and the achievement Report that industrialization is cultivated is because of belonging to secret character, the external world is difficult for learning content.And breadboard Wei Sheng Wu Fermentation ferment groove is equipped with accessory air compressor, steam boiler, frozen water machine, bucket groove, pipeline etc. again, looks the brand specification, needs 100 to 3,000,000 yuan of costs, and general user can't afford so huge expense at all.In addition, some user, farmers' or small-sized workshop produce the microorganism of agricultural or other purposes voluntarily with big plastic tank or metal bucket, but the program of sterilizing in advance because of no culture medium, the air that feeds does not also have the processing of degerming, there are not suitable stirring or temperature control device again, do not know so culturing process is contaminated, culture effect is also not good.As for the training method of small-sized self-control microorganism, almost there is not regular reported literature.Therefore the invention provides a kind of easy microbial cultivation equipment of sterilizing, it is simple in structure, and is with low cost, and processing ease, and can avoid living contaminants is to allow the user with the culturing micro-organisms and using voluntarily of mode cheaply.
Summary of the invention
One of purpose of the present invention is to provide a kind of easy microbial cultivation equipment and cultural method thereof of sterilizing, and it is simple in structure, to allow the user voluntarily with mode culturing micro-organisms cheaply and use.
One of purpose of the present invention, be to provide a kind of easy microbial cultivation equipment and cultural method thereof of sterilizing, it is so that bacterium is eliminated by an air filter that is arranged at a culture tank, and feed a gas by a ventpipe, gas is discharged via a venting port of culture tank, air in the culture tank is constantly discharged, pollute cultured microorganism to avoid bacterium to enter in the culture tank by the external world.
To achieve the above object, the present invention is a kind of easy microbial cultivation equipment of sterilizing, and comprises:
One culture tank has a venting port;
One whipping appts is arranged in the side of this culture tank;
One ventpipe is arranged in the side of this culture tank, and this ventpipe feeds a gas, and this gas is discharged via this venting port;
One air filter is located in this culture tank and is connected with this ventpipe;
One heating unit is located at the bottom of this culture tank; And
One refrigerating unit is located in this culture tank.
Among the present invention, wherein the material of this culture tank comprises iron and steel.
Among the present invention, wherein this culture tank has a venting port, and this venting port is provided with a vent cap.
Among the present invention, wherein this vent cap is provided with a pore.
Among the present invention, wherein this air filter comprises a filter membrane.
Among the present invention, wherein the material of this filter membrane comprises tetrafluoroethylene.
Among the present invention, wherein the hole of this filter membrane is the 0.18-0.26 micron.
Among the present invention, wherein this ventpipe has an air intake vent and air outlet, and this air intake vent is positioned at outside this culture tank, and this air outlet is positioned within this culture tank.
Among the present invention, wherein the position of this air intake vent is higher than the position of this air outlet.
Among the present invention, more comprise a Bubbled stone, this Bubbled stone is located in this culture tank and with this ventpipe and is connected.
Among the present invention, wherein this refrigerating unit more comprises a cooling tube, and this cooling tube has an input terminus and an output terminal, and the position of this input terminus is lower than the position of this output terminal.
Among the present invention, more comprise a thermometer, this thermometer is located at a side of this culture tank.
Among the present invention, wherein this whipping appts comprises:
One stirring motor is located at the outside of this culture tank;
One stir shaft is arranged in the side of this culture tank and connects with this stirring motor; And
One agitating vane is located at this stir shaft and is positioned at this culture tank.
Among the present invention, wherein this heating unit is a gas oven or other well heater.
The present invention also discloses a kind of cultural method of easy microbial cultivation equipment of sterilizing simultaneously, and it comprises:
(1) substratum boiling sterilization: substratum is placed in the culture tank, and heated and boiled;
(2) import air: import air before the substratum boiling sterilization finishes in the substratum that boils, this air is the air filter of being located in this culture tank through;
(3) cooling substratum;
(4) there is not assorted bacterium inoculation: will plant bacterium liquid and under not having assorted bacterium state, pour into and finish inoculation in the culture tank;
(5) keep " malleation " in cultivating operating period.
Among the present invention, wherein the assorted bacterium inoculation of this nothing of step (four) is that the vent cap of this culture tank is opened some, keeps sterile air under the state that opening is constantly discharged, and will plant bacterium liquid and pour in this culture tank and finish inoculation.
Among the present invention, wherein step (five) this to keep " malleation " mode be the state that only has sterile air to discharge from culture tank continue to import microbiological free air, to make this culture tank remain on, to lower contaminated chance.
The beneficial effect that the present invention has: easy microbial cultivation equipment of sterilizing of the present invention, not only simple in structure, cost of manufacture is cheap, to allow the user with the culturing micro-organisms and using voluntarily of mode cheaply, and by being arranged at air filter in the culture tank so that bacterium is eliminated, and make ventpipe feed the mode that gas is discharged via venting port again, and the air in the culture tank is constantly discharged, pollute cultured microorganism to avoid bacterium to enter in the culture tank by the external world.
Description of drawings
Fig. 1 is the side-view of the easy microbial cultivation equipment of sterilizing of preferred embodiment of the present invention; And
Fig. 2 is the top view of the easy microbial cultivation equipment of sterilizing of preferred embodiment of the present invention.
[figure number simple declaration]
10 culture tanks, 12 venting ports
14 vent cap, 142 pores
16 product flow export 20 whipping apptss
22 stirring motors, 24 stir shafts
26 agitating vanes, 30 ventpipes
32 air intake vents, 34 air outlets
36 air filters, 38 Bubbled stones
40 heating units, 50 refrigerating units
52 cooling tubes, 522 input terminuss
52 4 output terminals, 60 thermometers
Embodiment
Further understand and understanding for making constitutional features of the present invention and the effect reached had, cooperate detailed explanation, be described as follows in order to preferred embodiment and accompanying drawing:
See also Fig. 1 and Fig. 2, it is the side-view and the top view of the easy microbial cultivation equipment of sterilizing of preferred embodiment of the present invention; Easy microbial cultivation equipment of sterilizing of the present invention comprises a culture tank 10, a whipping appts 20, a ventpipe 30 and a heating unit 40; Culture tank 10 tops have a venting port 12; Whipping appts 20 is arranged in the side of culture tank 10; Ventpipe 30 is arranged in the side of culture tank 10, and ventpipe 30 feeds a gas by an air compressor, and gas can be air, and gas is discharged via exhaust 12; Heating unit 40 is located at the bottom of culture tank 10.
The present invention is that the example that is made as with the microorganism that contains the Su Li bacterium explains.And the material of making the culture tank 10 of microorganism can be iron and steel.Whipping appts 20 of the present invention comprises a stirring motor 22, a stir shaft 24 and an agitating vane 26, and stirring motor 22 is located at the outside of culture tank 10; Stir shaft 24 is arranged in the side of culture tank 10 and connects with stirring motor 22; Agitating vane 26 is located at stir shaft 24 and is positioned at culture tank 10.In addition, stirring motor 22 can connect again establishes a current transformer adjusting stirring motor 22 rotating speeds, and the present invention is adjusted into the revolution of stirring motor 22 about 300rpm.
The present invention makes the culture medium of microorganism can suitably allocate and adjust pH with molasses and amido acid solution, with culture medium pour into volume be about 100 liters culture tank 10 to about 7 one-tenth full.Culture medium can bubble in the process that stirs aerated culture, so add defoamer in advance as one feels fit.Then culture medium is boiled with gas oven heating unit 40.Heating unit 40 of the present invention can be a gas oven (or other well heater), and is provided with thermometer 60 in a side of culture tank 10.After the gas stove fire boils culture medium, then the gas stove fire is changeed little, and keep culture medium in boiling state, make culture medium boiling sterilization one hour.And but the vent cap 14 mat groove internal pressures of culture tank of the present invention 10 are opened voluntarily.The present invention more can be provided with pore 142 in vent cap 14, and vent cap 14 lids are tight, and the gas of culture tank 10 is constantly discharged by pore 142, therefore can make culture tank 10 keep semi-open state, discharges steaminess or air.
Ventpipe 30 of the present invention has an air intake vent 32 and air outlet 34, and air intake vent 32 is positioned at outside the culture tank 10, and air outlet 34 is positioned within the culture tank 10.The position of air intake vent 32 is higher than the position of air outlet 34.After so can making clean gas via ebullient culture medium heat sterilization, in culture medium, overflowing outside venting port 12, so can avoid bacterium by entering culture medium in the venting port 12, the position of air intake vent 32 also can be higher than the highest liquid level of culture medium usually, flows out from air intake vent 32 to avoid culture medium.
The present invention more is provided with an air filter 36 in culture tank 10, air filter 36 connects with ventpipe 30, use the gas that will be input into culture medium a membrane filtration, with airborne impurity of filtering or bacterium, to produce clean gas by air filter 36.The filter membrane material can be hydrophobic polytetrafluoroethylene (PTFE) or other material, and the hole of filter membrane is about 0.18-0.26 micron (as being preferably 0.22 micron), also visual actual demand and change the filter membrane of different aperture.During the culture medium heat sterilization, air filter 36 and air downstream pipeline thereof are subjected to heat sterilization in the lump.Finished preceding ten minutes in culture medium boiling sterilization, the unlatching air compressor pours into the air through dehumidifying, about 50～100 liters/minute of flow will may blow into fully sterilization in the ebullient culture medium still with the air of assorted bacterium in the pipeline on the air filter 36 downstream culture medium liquid levels.Again by the air outlet 34 aseptic air filled cavity of emerging, discharged by the pore 142 or the venting port 12 of the vent cap 14 of culture tank 10 again by sterile air behind air filter 36 and downstream pipeline thereof for airflow.Culture tank 10 finished to cultivating from this moment, keep always and pour into microbiological free air, and aseptic air is discharged from pore 142 or venting port 12, avoid with " malleation " in the air admission culture tank 10 of assorted bacterium, play to keep pouring into microbiological free air this moment, the culture medium suck-back is gone into air filter 36 and is damaged strainer in the time of also can avoiding follow-up culture medium to cool off always.
The present invention more comprises porous one Bubbled stone 38, and Bubbled stone 38 is located in the culture tank 10 and with ventpipe 30 and is connected, and Bubbled stone 38 has the porous crack, and in the time of can making clean gas squeeze into culture medium, it is more tiny and average that bubble becomes.
The present invention is provided with a refrigerating unit 50 in culture tank 10, refrigerating unit 50 more comprises a cooling tube 52, and cooling tube 52 is a screwed pipe, and cooling tube 52 has an input terminus 522 and an output terminal 524, and input terminus 522 all is positioned at outside the culture tank 10 with output terminal 524.The position of input terminus 522 is lower than the position of output terminal 524.After culture medium sterilization is finished, the input terminus 522 of cooling tube 52 is fed cooling liqss, water for example, and flow out from the output terminal 524 of cooling tube 52.So that culture medium is cooled to 30 ℃, the vent cap 14 of culture tank 10 is opened a crack by cooling tube 52, to keep sterile air by constantly discharging in the seam.In the last germ-free air flow that blows, bacterial classification bacterium liquid is poured in the culture tank 10 to finish inoculation.Build the vent cap 14 of culture tank 10, start whipping appts 20, and make stirring motor 22 keep about 300rpm.
Based on above-mentioned explanation, cultural method of the present invention comprises:
(1) substratum boiling sterilization: substratum is placed in this culture tank 10, and heated and boiled;
(2) import air: (for example preceding 10 minutes) import air in the substratum that boils before the substratum boiling sterilization finishes, and this air is the air filter 36 in this is located at culture tank 10;
(3) cooling substratum;
(4) there is not assorted bacterium inoculation: will plant bacterium liquid and under not having assorted bacterium state, pour in the culture tank 10 and finish inoculation; The vent cap 14 that is about to this culture tank 10 is opened some, keeps sterile air under the state that opening is constantly discharged, and will plant bacterium liquid and pour in this culture tank 10 and finish inoculation;
(5) keep " malleation " in cultivating operating period; Promptly contain the assorted enterable state of bacterium air, to lower contaminated chance to continue importing microbiological free air, to make this culture tank remain on only have sterile air not have the external world from the culture tank discharge.
In sum, the invention relates to a kind of easy microbial cultivation equipment and cultural method thereof of sterilizing, its equipment comprises a culture tank; The culture tank top has a venting port; One ventpipe is arranged in the side of culture tank, and ventpipe feeds a gas by an air compressor, and gas is discharged via venting port, and an air filter is located in the culture tank and with ventpipe and is connected.So when the culture tank heat sterilization, in passing the bacterium in the air filter is eliminated, and feed the mode that gas is discharged via venting port again by ventpipe, and the air in the culture tank is constantly discharged, pollute cultured microorganism to avoid bacterium to enter in the culture tank by the external world.And the present invention is simple in structure, and cost of manufacture is cheap, to allow the user with the culturing micro-organisms and using voluntarily of mode cheaply.
In sum, it only is a preferred embodiment of the present invention, be not to be used for limiting scope of the invention process, all equalizations of doing according to the described shape of claim scope of the present invention, structure, feature and spirit change and modify, and all should be included in the claim scope of the present invention.
Claims (17)
1. the easy microbial cultivation equipment that can sterilize is characterized in that, comprises:
One culture tank has a venting port;
One whipping appts is arranged in the side of this culture tank;
One ventpipe is arranged in the side of this culture tank, and this ventpipe feeds a gas, and this gas is discharged via this venting port;
One air filter is located in this culture tank and is connected with this ventpipe;
One heating unit is located at the bottom of this culture tank; And
One refrigerating unit is located in this culture tank.
2. as claim the 1 described easy microbial cultivation equipment of sterilizing, it is characterized in that wherein the material of this culture tank comprises iron and steel.
3. as claim the 1 described easy microbial cultivation equipment of sterilizing, it is characterized in that wherein this culture tank has a venting port, this venting port is provided with a vent cap.
4. as claim the 3 described easy microbial cultivation equipments of sterilizing, it is characterized in that wherein this vent cap is provided with a pore.
5. as claim the 1 described easy microbial cultivation equipment of sterilizing, it is characterized in that wherein this air filter comprises a filter membrane.
6. as claim the 5 described easy microbial cultivation equipments of sterilizing, it is characterized in that wherein the material of this filter membrane comprises tetrafluoroethylene.
7. as claim the 5 described easy microbial cultivation equipments of sterilizing, it is characterized in that wherein the hole of this filter membrane is the 0.18-0.26 micron.
8. as claim the 1 described easy microbial cultivation equipment of sterilizing, it is characterized in that wherein this ventpipe has an air intake vent and air outlet, this air intake vent is positioned at outside this culture tank, and this air outlet is positioned within this culture tank.
9. as claim the 8 described easy microbial cultivation equipments of sterilizing, it is characterized in that wherein the position of this air intake vent is higher than the position of this air outlet.
10. as claim the 1 described easy microbial cultivation equipment of sterilizing, it is characterized in that, more comprise a Bubbled stone, this Bubbled stone is located in this culture tank and with this ventpipe and is connected.
11. as claim the 1 described easy microbial cultivation equipment of sterilizing, it is characterized in that wherein this refrigerating unit more comprises a cooling tube, this cooling tube has an input terminus and an output terminal, the position of this input terminus is lower than the position of this output terminal.
12., it is characterized in that more comprise a thermometer, this thermometer is located at a side of this culture tank as claim the 1 described easy microbial cultivation equipment of sterilizing.
13., it is characterized in that wherein this whipping appts comprises as claim the 1 described easy microbial cultivation equipment of sterilizing:
One stirring motor is located at the outside of this culture tank;
One stir shaft is arranged in the side of this culture tank and connects with this stirring motor; And
One agitating vane is located at this stir shaft and is positioned at this culture tank.
14., it is characterized in that wherein this heating unit is a gas oven or other well heater as claim the 1 described easy microbial cultivation equipment of sterilizing.
15. the cultural method of the easy microbial cultivation equipment that can sterilize is characterized in that, it comprises:
(1) substratum boiling sterilization: substratum is placed in the culture tank, and heated and boiled;
(2) import air: import air before the substratum boiling sterilization finishes in the substratum that boils, this air is the air filter of being located in this culture tank through;
(3) cooling substratum;
(4) there is not assorted bacterium inoculation: will plant bacterium liquid and under not having assorted bacterium state, pour into and finish inoculation in the culture tank;
(5) keep " malleation " in cultivating operating period.
16. cultural method as claim the 15 described easy microbial cultivation equipments of sterilizing, it is characterized in that, wherein the assorted bacterium inoculation of this nothing of step (four) is that the vent cap of this culture tank is opened some, keep sterile air under the state that opening is constantly discharged, will plant bacterium liquid and pour in this culture tank and finish inoculation.
17. cultural method as claim the 15 described easy microbial cultivation equipments of sterilizing, it is characterized in that, wherein step (five) this to keep " malleation " mode be continue to import microbiological free air, making this culture tank remain on only has the state of sterile air from the culture tank discharge, to lower contaminated chance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010170015.4A CN102234606B (en) | 2010-05-04 | 2010-05-04 | Sterilizable and convenient microorganism culture equipment and culture method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010170015.4A CN102234606B (en) | 2010-05-04 | 2010-05-04 | Sterilizable and convenient microorganism culture equipment and culture method thereof |
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| Publication Number | Publication Date |
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| CN102234606A true CN102234606A (en) | 2011-11-09 |
| CN102234606B CN102234606B (en) | 2014-07-16 |
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| CN201010170015.4A Expired - Fee Related CN102234606B (en) | 2010-05-04 | 2010-05-04 | Sterilizable and convenient microorganism culture equipment and culture method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936162A (en) * | 2014-04-28 | 2014-07-23 | 沈阳环境科学研究院 | Artificial wetland microbial flora adjusting device |
| CN107151629A (en) * | 2017-04-25 | 2017-09-12 | 青岛天人环境股份有限公司 | The double rank desulfurizers of Intelligent biogas |
| CN108823090A (en) * | 2018-06-19 | 2018-11-16 | 重庆富农现代生物科技有限公司 | A kind of microbial bacterial agent preparation facilities system |
| CN108998353A (en) * | 2018-07-05 | 2018-12-14 | 曾小敏 | A kind of device for microculture |
| CN109097256A (en) * | 2018-07-05 | 2018-12-28 | 曾小敏 | Microbial cultivation device |
| CN109097257A (en) * | 2018-07-05 | 2018-12-28 | 曾小敏 | A kind of device for microculture |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936162A (en) * | 2014-04-28 | 2014-07-23 | 沈阳环境科学研究院 | Artificial wetland microbial flora adjusting device |
| CN103936162B (en) * | 2014-04-28 | 2015-09-09 | 沈阳环境科学研究院 | A kind of artificial swamp microorganism species setting device |
| CN107151629A (en) * | 2017-04-25 | 2017-09-12 | 青岛天人环境股份有限公司 | The double rank desulfurizers of Intelligent biogas |
| CN107151629B (en) * | 2017-04-25 | 2019-06-28 | 青岛天人环境股份有限公司 | The double rank desulfurizers of Intelligent biogas |
| CN108823090A (en) * | 2018-06-19 | 2018-11-16 | 重庆富农现代生物科技有限公司 | A kind of microbial bacterial agent preparation facilities system |
| CN108998353A (en) * | 2018-07-05 | 2018-12-14 | 曾小敏 | A kind of device for microculture |
| CN109097256A (en) * | 2018-07-05 | 2018-12-28 | 曾小敏 | Microbial cultivation device |
| CN109097257A (en) * | 2018-07-05 | 2018-12-28 | 曾小敏 | A kind of device for microculture |
| CN109097256B (en) * | 2018-07-05 | 2021-12-21 | 上海天信绿色食品有限公司 | Microorganism culture device |
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| Publication number | Publication date |
|---|---|
| CN102234606B (en) | 2014-07-16 |
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