CN211645269U

CN211645269U - A culture apparatus for cardiac muscle cell

Info

Publication number: CN211645269U
Application number: CN201922311322.9U
Authority: CN
Inventors: 李思熳; 李树德; 李治纲
Original assignee: Kunming Medical University
Current assignee: Kunming Medical University
Priority date: 2019-12-20
Filing date: 2019-12-20
Publication date: 2020-10-09
Anticipated expiration: 2029-12-20

Abstract

The utility model discloses a culture apparatus for cardiac muscle cell relates to the culture apparatus field, including the case, the top of case has been seted up and has been placed the recess, and the inner chamber of placing the recess is equipped with the loading board, and the top of loading board is rectangular array spaced apart and is equipped with a plurality of spacing holes, and the top of loading board has a plurality of culture dishes through a plurality of spacing hole activity joint, and the bottom of culture dish passes spacing hole and extends to the inner chamber of placing the recess, places the fixed feed liquor pipe that alternates of recess one side inner wall, places recess opposite side inner wall fixed interlude and is connected with the fluid-discharge tube. The utility model discloses utilize the case, place the setting of recess, loading board, spacing hole and culture dish, can make the experimenter take out single culture dish, then carry out the planting of cardiomyocyte in the culture dish, concentrate at last and collect and concentrate the cultivation in the case, saved the case and need take everywhere the loaded down with trivial details of removal, improved the convenience that the cardiomyocyte cultivateed.

Description

A culture apparatus for cardiac muscle cell

Technical Field

The utility model relates to a culture apparatus field, in particular to a culture apparatus for cardiac muscle cell.

Background

At present to the culture of cardiomyocyte all adopt culture plate or blake bottle as culture apparatus to use in the laboratory, and the culture plate that cardiomyocyte culture used is mostly direct to set up a plurality of circular recesses at the equidistance on a transparent glass board at equal intervals and deposit cardiomyocyte, though can carry out multiunit cardiomyocyte's culture like this, all need take whole culture dish when the cardiomyocyte of different groups of access at every turn and carry out the access of other groups's cardiomyocyte, or use a plurality of culture plates, not only influence the culture effect of other cardiomyocytes easily, and the easy danger that falls of increase of the culture plate of taking frequently, thereby the convenience that the culture plate used has been reduced.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a culture apparatus for cardiomyocyte to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a culture apparatus for myocardial cell, includes the case, the recess has been seted up on the top of case, the inner chamber of placing the recess is equipped with the loading board, the top of loading board is the spaced apart a plurality of spacing holes that are equipped with of rectangular array, the top of loading board has a plurality of culture dishes through a plurality of spacing hole activity joint, just the bottom of culture dish is passed spacing hole and is extended to the inner chamber of placing the recess, it is connected with the feed liquor pipe to place the fixed interlude of recess one side inner wall, it is connected with the fluid-discharge tube to place the fixed interlude of recess opposite side inner wall.

Preferably, one end of each of the liquid inlet pipe and the liquid outlet pipe penetrates through the outer wall of the storage box and is connected with a pipe plug in a sealing and clamping mode.

Preferably, the top of placing the inner wall of the groove is four rubber strips fixedly connected with a rectangular array, the side wall of the bearing plate is annularly provided with a clamping groove, and the clamping groove is movably clamped with the four rubber strips respectively.

Preferably, the culture dish comprises an annular circular plate and a storage tube, the annular circular plate is fixedly sleeved on the top of the outer wall of the storage tube, and a cover plate is arranged at the top end of the annular circular plate.

Preferably, one side of the annular circular plate is provided with a concave groove, an inner cavity of the concave groove is rotatably connected with a fixing piece through a pin shaft, and the top end of the fixing piece is fixedly connected with one side of the bottom end of the cover plate.

Preferably, the middle parts of the two sides of the storage box are provided with concave clamping grooves, and the storage box, the bearing plate and the culture dish are made of transparent materials.

The utility model discloses a technological effect and advantage:

1. the utility model utilizes the arrangement of the storage box, the placing groove, the bearing plate, the limiting hole and the culture dish, so that an experimenter can take out a single culture dish, then the planting of the myocardial cells in the culture dish is carried out, and finally the myocardial cells are collected in the storage box in a centralized way for centralized culture, thereby saving the trouble that the storage box needs to be taken and moved everywhere and improving the convenience of the culture of the myocardial cells;

2. the utility model discloses utilize rubber strip and draw-in groove and setting, can be convenient for dismantlement and installation between loading board and the case fixed to be convenient for carry out cleaning and disinfection to case or loading board alone, improve the experimenter to the cleaning and disinfecting convenience of case or loading board.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a schematic view of the overall exploded structure of the present invention.

Fig. 3 is a schematic view of a part of an enlarged structure of fig. 2 according to the present invention.

Fig. 4 is a schematic front sectional view of the culture dish of the present invention.

In the figure: 1. a storage box; 2. placing a groove; 3. a carrier plate; 4. a limiting hole; 5. a culture dish; 6. a rubber strip; 7. a card slot; 8. a liquid inlet pipe; 9. a liquid discharge pipe; 10. a pipe plug; 11. an annular circular plate; 12. a storage tube; 13. a cover plate; 14. slotting in a concave manner; 15. a fixing member; 16. and (7) a pin shaft.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model provides a culture device for myocardial cells as shown in figures 1-4, which comprises a storage box 1, wherein the top end of the storage box 1 is provided with a placing groove 2 which plays a role of a storage space and is convenient for bearing and placing other components, the inner cavity of the placing groove 2 is provided with a bearing plate 3, the top part of the inner wall of the placing groove 2 is fixedly connected with four rubber strips 6 in a rectangular array, the side wall of the bearing plate 3 is annularly provided with a clamping groove 7, the clamping groove 7 is movably clamped with the four rubber strips 6 respectively, the rubber strips 6 and the clamping grooves 7 are matched for use, the assembly and disassembly between the bearing plate 3 and the storage box 1 can be convenient, thereby being convenient for cleaning and disinfecting the inner cavity of the placing groove 2, the top end of the bearing plate 3 is provided with a plurality of limiting holes 4 at intervals in the rectangular array for bearing and limiting the culture dishes 5, thereby being convenient for placing, the top end of the bearing plate 3 is movably clamped with a plurality of culture dishes 5 through a plurality of limiting holes 4 and used for storing myocardial cells, the bottom ends of the culture dishes 5 penetrate through the limiting holes 4 and extend to the inner cavities of the placing grooves 2, in order to prevent the culture dishes 5 from shaking in the placing grooves 2, the length of the culture dishes 5 can be set to be the same as the height of the placing grooves 2, after the culture dishes 5 are inserted into the limiting holes 4, the bottom ends of the culture dishes 5 are attached to the bottom ends of the placing grooves 2, the inner wall of one side of the placing groove 2 is fixedly and alternately connected with a liquid inlet pipe 8, the inner wall of the other side of the placing groove 2 is fixedly and alternately connected with a liquid outlet pipe 9, the arrangement of the liquid inlet pipe 8 and the liquid outlet pipe 9 can facilitate the addition of cooling liquid into the placing groove 2, so as to carry out heat preservation on the culture dishes 5, one ends of the liquid inlet, the liquid inlet pipe 8 or the liquid outlet pipe 9 is sealed, so that the liquid inlet pipe 8 or the liquid outlet pipe 9 can be opened or closed conveniently.

Culture dish 5 comprises annular plectane 11 and deposit pipe 12, annular plectane 11 is fixed to cup joint in the top of depositing pipe 12 outer wall, annular plectane 11's top is equipped with apron 13, concave type fluting 14 has been seted up to one side of annular plectane 11, concave type fluting 14's inner chamber rotates through round pin 16 and is connected with mounting 15, and one side fixed connection of the top of mounting 15 and apron 13 bottom, one side through manual lift-up apron 13, make apron 13 rotate around the round pin 16 of concave type fluting 14 inner chamber through mounting 15, thereby be convenient for opening of apron 13 to close, thereby prevent that the cardiomyocyte in the culture dish 5 from unrestrained.

Concave type draw-in groove has all been seted up at the middle part of case 1 both sides, and the experimenter of being convenient for takes case 1, and case 1, loading board 3 and culture dish 5 are transparent material, and the experimenter of being convenient for observes the culture condition of the interior cardiomyocyte of culture dish 5.

This practical theory of operation: when carrying out the cultivation of cardiomyocyte, take away corresponding culture dish 5 from loading board 3 for each group of experimenters, then carry out the planting of cardiomyocyte at 5 inner chambers of culture dish, after the completion is planted to the cardiomyocyte in culture dish 5, each group of experimenters only need insert culture dish 5 in the spacing hole 4 on loading board 3 to the label that corresponds can be pasted on 13 tops of apron on culture dish 5, at last with whole case 1 put into the cell culture case cultivate can.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. A culture device for cardiomyocytes, comprising a storage case (1), characterized in that: the top of case (1) has been seted up and has been placed recess (2), the inner chamber of placing recess (2) is equipped with loading board (3), the top of loading board (3) is the rectangular array interval and has been seted up a plurality of spacing holes (4), the top of loading board (3) has a plurality of culture dishes (5) through a plurality of spacing hole (4) activity joint, just the bottom of culture dish (5) is passed spacing hole (4) and is extended to the inner chamber of placing recess (2), it is connected with feed liquor pipe (8) to place recess (2) one side inner wall fixed interlude, it is connected with fluid-discharge tube (9) to place recess (2) opposite side inner wall fixed interlude.

2. A culture apparatus for cardiomyocytes according to claim 1, wherein: one end of the liquid inlet pipe (8) and one end of the liquid outlet pipe (9) both penetrate through the outer wall of the storage box (1) and are connected with pipe plugs (10) in a sealing and clamping mode.

3. A culture apparatus for cardiomyocytes according to claim 1, wherein: the top of placing recess (2) inner wall is four rubber strips (6) of rectangular array fixedly connected with, draw-in groove (7) have been seted up to the lateral wall ring shape of loading board (3), just draw-in groove (7) respectively with four rubber strip (6) activity joint.

4. A culture apparatus for cardiomyocytes according to claim 1, wherein: the culture dish (5) comprises annular circular plate (11) and deposit pipe (12), fixed cover of annular circular plate (11) is in the top of depositing pipe (12) outer wall, the top of annular circular plate (11) is equipped with apron (13).

5. A culture apparatus for cardiomyocytes according to claim 4, wherein: a concave groove (14) is formed in one side of the annular circular plate (11), a fixing piece (15) is rotatably connected to the inner cavity of the concave groove (14) through a pin shaft (16), and the top end of the fixing piece (15) is fixedly connected with one side of the bottom end of the cover plate (13).

6. A culture apparatus for cardiomyocytes according to claim 1, wherein: concave type draw-in groove has all been seted up at the middle part of case (1) both sides, just case (1), loading board (3) and culture dish (5) are transparent material.