CN108239599B - Air bacteria sampling, culturing and detecting integrated device - Google Patents
Air bacteria sampling, culturing and detecting integrated device Download PDFInfo
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- CN108239599B CN108239599B CN201810254356.6A CN201810254356A CN108239599B CN 108239599 B CN108239599 B CN 108239599B CN 201810254356 A CN201810254356 A CN 201810254356A CN 108239599 B CN108239599 B CN 108239599B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
Abstract
The invention discloses an air bacteria sampling, culturing and detecting integrated device, which comprises a base, a sampling box, an incubator and a detecting box, wherein the sampling box, the incubator and the detecting box are all arranged on the base and are separated by a partition plate, a sampling pipe is arranged in the sampling box, a sampling pump is arranged on the sampling pipe, a sampling nozzle is arranged at the outer opening of the sampling pipe, a sealing cover is arranged on the sampling nozzle, a biochemical incubator is arranged in the incubator, a base is arranged in the biochemical incubator, a micro-vibrator is arranged on the surface of the base, a culture dish is arranged on the micro-vibrator, a lighting lamp is also arranged at the top of the biochemical incubator, a detecting platform is arranged at the bottom of the detecting box, a detecting dish and a micro-lens are arranged on the detecting platform, a processing device is arranged right above the detecting dish, and a filtering membrane and adsorption paper are arranged in the processing device. Sterile culture and accurate detection.
Description
Technical Field
The invention relates to the technical field of bacteria culture, in particular to an air bacteria sampling, culturing and detecting integrated device.
Background
bacteria are one of the main groups of organisms, belonging to the bacterial domain. Is also the most abundant class of all organisms, and it is estimated that there are approximately 5 x 10^30 in total. The shape of the bacteria is quite diverse, mainly spherical, rod-like, and spiral. Bacteria also have a great influence on human activities. On the one hand, bacteria are the causative agents of many diseases, including tuberculosis, gonorrhea, anthrax, syphilis, plague, trachoma, etc. are all caused by bacteria. However, the use of bacteria by humans is also frequent, for example in the manufacture of cheese and yoghurt and fermented glutinous rice, the manufacture of some antibiotics, the treatment of waste water, etc., all in relation to bacteria. Bacteria are also widely used in the field of biotechnology. Bacterial culture is a technique in which bacteria are grown and propagated artificially. The bacteria are widely distributed in nature, large in quantity and multiple in types, can benefit mankind, and can also become a cause of diseases. Most bacteria can be cultured artificially by inoculating them on a culture medium and allowing them to grow and propagate. The cultured bacteria are used for research, identification and application. Bacterial culture is a complex technique. The existing bacterium research device on the market has the defects of single function, complex sampling, inaccurate detection and the like.
Disclosure of Invention
The invention aims to provide an air bacteria sampling, culturing and detecting integrated device to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: an air bacteria sampling, culturing and detecting integrated device comprises a base, a sampling box, an incubator and a detecting box, wherein the sampling box, the incubator and the detecting box are arranged on the upper surface of the base, a first clapboard is arranged between the sampling box and the incubator, a second clapboard is arranged between the incubator and the detecting box, a sampling pipe is arranged inside the sampling box, the sampling pipe is horizontally arranged, a sampling pump is arranged above the sampling pipe, a sampling nozzle is vertically arranged at the left end of the sampling pipe, the sampling nozzle is flush with the left side surface of the sampling box, a sealing cover is vertically arranged outside the sampling nozzle, the diameter of the sealing cover is slightly larger than that of the sampling nozzle, a first guide plate and a second guide plate are arranged on the right side of the sampling pipe, the first guide plate and the second guide plate are arranged in a laterally-arranged 'eight' shape, and an opening formed by the first guide plate and the second guide plate is flush with the central point of the sampling pipe in the horizontal direction, sampling bottom of the case portion still is equipped with the sampling bottle, the sampling bottle constitutes for colorless transparent borosilicate glass, be equipped with the cork on the sampling bottle opening, be equipped with biochemical incubator in the incubator, the incubator medial surface all is equipped with the heat preservation, the detection bottom of the case portion is equipped with testing platform, testing platform sets up in the intermediate position, the testing platform upper surface is equipped with the detection ware, the detection ware constitutes for colorless transparent borosilicate glass, it is equipped with the microscope camera lens to detect the ware right side, be equipped with processing apparatus directly over the detection ware, processing apparatus is fixed in the bottom of detection case through first stabilizer blade and second stabilizer blade, the processing apparatus top is equipped with puts in the mouth.
Preferably, the inside driving motor that still is equipped with of sampling case, the driving motor right flank is equipped with the power, the driving motor left surface is equipped with the drive pivot, drive pivot left end is equipped with the sampling face, sampling face left surface is equipped with the sampling ware, the sampling ware constitutes for colorless transparent borosilicate glass, the height of sampling ware and the height of sampling face equal, the sampling ware sets up directly over the sampling bottle.
preferably, biochemical incubator bottom is equipped with the base, the base upper surface is equipped with the microseismic ware, be equipped with the culture dish on the microseismic ware, the culture dish constitutes for colorless transparent borosilicate glass, biochemical incubator top is equipped with the light, the light sets up directly over the culture dish, the left surface of biochemical incubator is equipped with the thermometer, the inside temperature of biochemical incubator sets up to 37 ℃, the outside of biochemical incubator still is equipped with the viewing aperture, the viewing aperture constitutes for transparent glass.
Preferably, a filtering membrane and adsorption paper are arranged in the treatment device, the filtering membrane and the adsorption paper are horizontally arranged, the width of the filtering membrane and the width of the adsorption paper are equal to the width of the treatment device, the filtering membrane is arranged above the adsorption paper, the filtering membrane is a hydrophilic polysulfone filtering membrane with an equivalent filtering pore size of 0.2 μm, and the adsorption paper is made of super absorbent resin.
Preferably, a micropore filter screen is arranged at the opening of the sampling nozzle.
preferably, a glucose culture medium is arranged in the culture dish.
Preferably, a first through hole is formed in the middle of the first partition plate, and a second through hole is formed in the middle of the second partition plate.
Preferably, sampling box and incubator bottom are equipped with miniature pump, the miniature pump right side is equipped with the empty storehouse, the width in empty storehouse equals with the width of detection case, the inside sterilization jar that is equipped with of empty storehouse, the sterilization jar passes through pipe connection and detects the ware, be equipped with the control valve on the pipe.
Preferably, from top to bottom be equipped with four guide plates, fifth guide plate and third guide plate in the sterilization tank, sterilization tank left side inner wall is equipped with first ultraviolet sterilamp, sterilization tank right side inner wall is equipped with second ultraviolet sterilamp and third ultraviolet sterilamp, first ultraviolet sterilamp and fifth guide plate flush, second ultraviolet sterilamp and fourth guide plate flush, third ultraviolet sterilamp and third guide plate flush, the sterilization tank bottom is equipped with the discharge port.
Preferably, the method of use comprises the steps of:
A. In the sampling box, air in an environment needing to be detected is extracted through a sampling pump, and dust is filtered out of the air through a sampling nozzle;
B. The bacteria enter the flow guide port through the sampling pipe, the bacteria pass through the flow guide port and are uniformly dispersed on the rotating sampling vessel, and the sampling vessel can be temporarily stored through a sampling bottle;
C. in the incubator, sample bacteria are put into a culture dish containing a glucose culture medium, a lighting lamp above the culture dish is favorable for light culture, the sample bacteria are quickly cultured at the most proper temperature of 37 ℃, and the bacteria culture medium can be overturned by shaking of a micro-vibrator regularly in the process, so that the growth speed of the bacteria is accelerated;
D. in the detection case, the culture medium that contains the sample bacterium gets into processing apparatus through putting in mouthful, filters through filtration membrane, gets rid of unnecessary culture medium through adsorbing paper, arranges the test dish in at last, can observe the situation of sample bacterium through the microscope camera lens.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention has simple principle and compact structure, integrates bacteria sampling, culturing and detecting into a whole, simplifies the bacteria research work and improves the utilization rate of the bacteria sampling, culturing and detecting integrated device.
(2) The invention is provided with the microporous filter screen at the opening of the sampling nozzle, so that dust in the air can be removed preliminarily.
(3) according to the invention, the sampling vessel is arranged on the rotating motor, so that bacteria in the air can be uniformly collected.
(4) The temperature in the biochemical incubator is set to 37 ℃, and the most appropriate temperature is provided for bacterial culture.
(5) The top of the biochemical incubator is provided with the illuminating lamp, which is beneficial to the light and culture of bacteria.
(6) according to the invention, the micro-vibrator is arranged below the culture dish, and the bacteria can be overturned through vibration, so that the speed of bacteria culture can be improved.
(7) the treatment device is internally provided with the filter membrane and the adsorption paper, so that microorganisms in the sample can be filtered, and redundant culture medium in the sample can be removed.
(8) The invention is provided with the sterilization tank, can sterilize the culture medium containing the bacterial sample, and then discharges the culture medium, thereby avoiding causing environmental pollution.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of a sampling nozzle of the present invention;
fig. 3 is a schematic view of a sterilization tank of the present invention.
Detailed Description
the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: an air bacteria sampling, culturing and detecting integrated device comprises a base 8, a sampling box 1, an incubator 2 and a detecting box 3, wherein the sampling box 1, the incubator 2 and the detecting box 3 are arranged on the upper surface of the base 8, a first baffle 16 is arranged between the sampling box 1 and the incubator 2, a second baffle 17 is arranged between the incubator 2 and the detecting box 3, a sampling pipe 5 is arranged in the sampling box 1, the sampling pipe 5 is horizontally arranged, a sampling pump 4 is arranged above the sampling pipe 5, a sampling nozzle 6 is vertically arranged at the left end of the sampling pipe 5, the sampling nozzle 6 is flush with the left side surface of the sampling box 1, a sealing cover 7 is vertically arranged at the outer side of the sampling nozzle 6, the diameter of the sealing cover 7 is slightly larger than that of the sampling nozzle 6, a first guide plate 9 and a second guide plate 10 are arranged at the right side of the sampling pipe 5, the first guide plate 9 and the second guide plate 10 are arranged in a shape of a side-arranged 'eight', the opening that first guide plate 9 and second guide plate 10 formed flushes in the horizontal direction with sampling pipe 5 central point, sampling case 1 bottom still is equipped with sampling bottle 18, sampling bottle 18 constitutes for colorless transparent borosilicate glass, be equipped with cork 19 on the sampling bottle 18 opening, be equipped with biochemical incubator 21 in the incubator 2, incubator 2 medial surface all is equipped with heat preservation 20, detection case 3 bottom is equipped with detection platform 33, detection platform 33 sets up in the intermediate position, detection platform 33 upper surface is equipped with detection dish 34, detection dish 34 constitutes for colorless transparent borosilicate glass, detection dish 34 right side is equipped with microscope lens 35, be equipped with processing apparatus 27 directly over detection dish 34, processing apparatus 27 is fixed in the bottom of detection case 3 through first stabilizer blade 31 and second stabilizer blade 32, processing apparatus 27 top is equipped with puts in mouthful 28, the device simplifies the bacteria research work and improves the utilization rate of the bacteria sampling, culturing and detecting integrated device.
In this embodiment, sampling case 1 is inside still to be equipped with driving motor 12, driving motor 12 right flank is equipped with power 11, driving motor 12 left flank is equipped with drive pivot 13, the 13 left ends of drive pivot are equipped with sampling face 14, the 14 left flanks of sampling face is equipped with sampling dish 15, sampling dish 15 constitutes for colorless transparent borosilicate glass, and the bacterium in the even collection air can be guaranteed to the rotatory face of gathering, the height of sampling dish 15 and the height of sampling face 14 equal, sampling dish 15 sets up directly over sampling bottle 18.
In this embodiment, biochemical incubator 21 bottom is equipped with base 22, base 22 upper surface is equipped with microseismic ware 23, be equipped with culture dish 24 on the microseismic ware 23, through vibrations upset bacterium, can improve bacterial cultivation's speed, culture dish 24 constitutes for colorless transparent borosilicate glass, biochemical incubator 21 top is equipped with light 37, light 37 sets up directly over culture dish 24, is favorable to the light and the cultivation of bacterium, biochemical incubator 21's left surface is equipped with thermometer 25, biochemical incubator 21's inside temperature sets up to 37 ℃, provides the most suitable temperature for bacterial cultivation, biochemical incubator 21's the outside still is equipped with viewing aperture 26, viewing aperture 26 constitutes for transparent glass.
In this embodiment, a filtering membrane 29 and an adsorbing paper 30 are arranged in the processing device 27, the filtering membrane 29 and the adsorbing paper 30 are horizontally arranged, the widths of the filtering membrane 29 and the adsorbing paper 30 are equal to the width of the processing device 27, the filtering membrane 29 is arranged above the adsorbing paper 30, the filtering membrane 29 is a hydrophilic polysulfone filtering membrane with an equivalent filtering pore size of 0.2um, so that microorganisms in a sample can be filtered, and the adsorbing paper 30 is made of super absorbent resin so as to remove redundant culture medium in the sample.
In this embodiment, the opening of the sampling nozzle 6 is provided with a microporous filter screen 36, so that dust in the air can be removed preliminarily.
In this embodiment, a glucose medium is provided in the culture dish 24.
in this embodiment, the first partition 16 has a first through hole 38 at the middle portion thereof, and the second partition 17 has a second through hole 39 at the middle portion thereof.
In this embodiment, sampling box 1 and incubator 2 bottom are equipped with micro-water pump 40, micro-water pump 40 right side is equipped with empty storehouse 41, the width of empty storehouse 41 and detection case 3's width equal, the inside sterilization jar 42 that is equipped with of empty storehouse 41, sterilization jar 42 passes through pipe 43 and connects detection ware 34, be equipped with control valve 44 on the pipe 43.
In this embodiment, from top to bottom be equipped with fourth guide plate 45, fifth guide plate 46 and third guide plate 47 in the sterilization jar 42, sterilization jar 42 left side inner wall is equipped with first ultraviolet sterilamp 48, sterilization jar 42 right side inner wall is equipped with second ultraviolet sterilamp 49 and third ultraviolet sterilamp 50, first ultraviolet sterilamp 48 flushes with fifth guide plate 46, second ultraviolet sterilamp 49 flushes with fourth guide plate 45, third ultraviolet sterilamp 50 flushes with third guide plate 47, sterilization jar 42 bottom is equipped with discharge port 51, and the sterilization jar can carry out sterilization treatment to the culture medium that contains the bacterium sample, then discharges, avoids causing environmental pollution.
The working principle of the invention is as follows: in the sampling box, need the air among the detection environment through the sampling pump extraction, the air filters the dust through the sampling mouth, get into the water conservancy diversion mouth through the sampling pipe, the sample bacterium passes water conservancy diversion mouth and evenly spreads on rotatory sampling dish, the sampling dish can be preserved temporarily through the sampling bottle, in the incubator, the sample bacterium is put into the culture dish that contains the glucose culture medium, the light of culture dish top is favorable to light and cultivates, the sample bacterium cultivates fast under most suitable 37 ℃, the in-process can be regularly through the shake upset bacterial culture medium of microseismator, accelerate bacterium growth speed, in the detection case, the culture medium that contains the sample bacterium gets into processing apparatus through putting in the mouth, filter through filtration membrane, get rid of unnecessary culture medium through adsorbing paper, arrange in the detection dish finally, can observe the situation of sample bacterium through the microscope camera lens.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. a use method of an air bacteria sampling, culturing and detecting integrated device is characterized in that: the air bacteria sampling, culturing and detecting integrated device comprises a base (8), a sampling box (1), an incubator (2) and a detection box (3), wherein the sampling box (1), the incubator (2) and the detection box (3) are arranged on the upper surface of the base (8), a first baffle plate (16) is arranged between the sampling box (1) and the incubator (2), a second baffle plate (17) is arranged between the incubator (2) and the detection box (3), a sampling pipe (5) is arranged inside the sampling box (1), the sampling pipe (5) is horizontally arranged, a sampling pump (4) is arranged above the sampling pipe (5), a sampling nozzle (6) is vertically arranged at the left end of the sampling pipe (5), the left side surface of the sampling nozzle (6) and the left side surface of the sampling box (1) are flush, a sealing cover (7) is vertically arranged outside the sampling nozzle (6), and the diameter of the sealing cover (7) is slightly larger than that of the sampling nozzle (6), the right side of sampling pipe (5) is equipped with first guide plate (9) and second guide plate (10), first guide plate (9) and second guide plate (10) are the setting of "eight" style of calligraphy of side-putting, the opening that first guide plate (9) and second guide plate (10) formed flushes on the horizontal direction with sampling pipe (5) central point, sampling case (1) bottom still is equipped with sampling bottle (18), sampling bottle (18) constitute for colorless transparent borosilicate glass, be equipped with cork (19) on sampling bottle (18) opening, be equipped with biochemical incubator (21) in incubator (2), incubator (2) medial surface all is equipped with heat preservation (20), detection case (3) bottom is equipped with detection platform (33), detection platform (33) set up in the intermediate position, detection platform (33) upper surface is equipped with detection dish (34), the detection vessel (34) is made of colorless transparent borosilicate glass, a microscope lens (35) is arranged on the right side of the detection vessel (34), a processing device (27) is arranged right above the detection vessel (34), the processing device (27) is fixed at the bottom of the detection box (3) through a first supporting leg (31) and a second supporting leg (32), and a putting-in opening (28) is formed in the top of the processing device (27);
The sampling box (1) is internally provided with a driving motor (12), the right side face of the driving motor (12) is provided with a power supply (11), the left side face of the driving motor (12) is provided with a driving rotating shaft (13), the left end of the driving rotating shaft (13) is provided with a sampling surface (14), the left side face of the sampling surface (14) is provided with a sampling vessel (15), the sampling vessel (15) is made of colorless transparent borosilicate glass, the height of the sampling vessel (15) is equal to that of the sampling surface (14), and the sampling vessel (15) is arranged right above the sampling bottle (18);
The biochemical incubator (21) is provided with a base (22) at the bottom, a micro-vibrator (23) is arranged on the upper surface of the base (22), a culture dish (24) is arranged on the micro-vibrator (23), the culture dish (24) is made of colorless transparent borosilicate glass, an illuminating lamp (37) is arranged at the top of the biochemical incubator (21), the illuminating lamp (37) is arranged right above the culture dish (24), a thermometer (25) is arranged on the left side surface of the biochemical incubator (21), the internal temperature of the biochemical incubator (21) is set to be 37 ℃, an observation port (26) is further arranged on the outer side of the biochemical incubator (21), and the observation port (26) is made of transparent glass;
A filtering membrane (29) and adsorption paper (30) are arranged in the treatment device (27), the filtering membrane (29) and the adsorption paper (30) are horizontally arranged, the widths of the filtering membrane (29) and the adsorption paper (30) are equal to the width of the treatment device (27), the filtering membrane (29) is arranged above the adsorption paper (30), the filtering membrane (29) is a hydrophilic polysulfone filtering membrane with the equivalent filtering pore diameter of 0.2 mu m, and the adsorption paper (30) is made of super absorbent resin;
The use method of the air bacteria sampling, culturing and detecting integrated device comprises the following steps:
A. In the sampling box, air in an environment needing to be detected is extracted through a sampling pump, and dust is filtered out of the air through a sampling nozzle;
B. The bacteria enter the flow guide port through the sampling pipe, the bacteria pass through the flow guide port and are uniformly dispersed on the rotating sampling vessel, and the sampling vessel can be temporarily stored through a sampling bottle;
C. In the incubator, sample bacteria are put into a culture dish containing a glucose culture medium, a lighting lamp above the culture dish is favorable for light culture, the sample bacteria are quickly cultured at the most proper temperature of 37 ℃, and the bacteria culture medium can be overturned by shaking of a micro-vibrator regularly in the process, so that the growth speed of the bacteria is accelerated;
D. in the detection case, the culture medium that contains the sample bacterium gets into processing apparatus through putting in mouthful, filters through filtration membrane, gets rid of unnecessary culture medium through adsorbing paper, arranges the test dish in at last, can observe the situation of sample bacterium through the microscope camera lens.
2. The method of claim 1, further comprising: and a micropore filter screen (36) is arranged at the opening of the sampling nozzle (6).
3. The method of claim 1, further comprising: the middle of the first clapboard (16) is provided with a first through hole (38), and the middle of the second clapboard (17) is provided with a second through hole (39).
4. The method of claim 1, further comprising: sampling case (1) and incubator (2) bottom are equipped with miniature pump (40), miniature pump (40) right side is equipped with empty storehouse (41), the width of empty storehouse (41) equals with the width of detection case (3), empty storehouse (41) inside is equipped with sterilization jar (42), sterilization jar (42) are connected detection ware (34) through pipe (43), be equipped with control valve (44) on pipe (43).
5. The method of claim 4, further comprising: from top to bottom be equipped with fourth guide plate (45), fifth guide plate (46) and third guide plate (47) in sterilization jar (42), sterilization jar (42) left side inner wall is equipped with first ultraviolet sterilamp (48), sterilization jar (42) right side inner wall is equipped with second ultraviolet sterilamp (49) and third ultraviolet sterilamp (50), first ultraviolet sterilamp (48) and fifth guide plate (46) flush, second ultraviolet sterilamp (49) and fourth guide plate (45) flush, third ultraviolet sterilamp (50) and third guide plate (47) flush, sterilization jar (42) bottom is equipped with discharge port (51).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810254356.6A CN108239599B (en) | 2018-03-26 | 2018-03-26 | Air bacteria sampling, culturing and detecting integrated device |
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| CN201810254356.6A CN108239599B (en) | 2018-03-26 | 2018-03-26 | Air bacteria sampling, culturing and detecting integrated device |
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| CN108239599A CN108239599A (en) | 2018-07-03 |
| CN108239599B true CN108239599B (en) | 2019-12-13 |
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| CN2301722Y (en) * | 1997-03-24 | 1998-12-23 | 戴景林 | Three-stage impact multifunctional air sampler |
| CN203625187U (en) * | 2013-10-25 | 2014-06-04 | 内蒙古蒙水水资源股份有限公司 | Integrated sewage treatment device |
| CN104946520A (en) * | 2015-07-02 | 2015-09-30 | 济南大学 | Biological detection, collection and culture device |
| CN205528803U (en) * | 2016-02-18 | 2016-08-31 | 宁波大学 | Microbiological incubator of circulation controllable temperature |
| CN107118961B (en) * | 2017-05-16 | 2020-06-16 | 上海德具生物科技有限公司 | Online collection and online automatic detection device for bacteria or viruses in air |
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