CN2169629Y

CN2169629Y - Disposable micro porous filtering and cultivating device

Info

Publication number: CN2169629Y
Application number: CN 93210917
Authority: CN
Inventors: 叶大林
Original assignee: Individual
Current assignee: Individual
Priority date: 1993-04-24
Filing date: 1993-04-24
Publication date: 1994-06-22
Anticipated expiration: 2003-04-24

Abstract

The utility model discloses a disposable micro porous filtering and cultivating device which is used for filtering and bacterium-collecting of liquid samples and in which the bacteria is cultivated. The utility model is made of micro porous filtering film and transparent plastic, and is provided with a controllable path whose top and bottom are communicated and two four-way structures. Three filtering and cultivating devices can be used for evenly filtering simultaneously. After the filtering, the controllable path is opened, the culture media are sucked, and the bacteria cultivation can be carried out. The utility model is suitable for the sterility tests of the bacterium-collecting cultivated liquid samples, and a single filtering and cultivating device can be used as a sterilization filter.

Description

Disposable micro porous filtering and cultivating device

The utility model relates to the device of liquid filtering and microbial culture.

With the immediate prior art of the utility model be disposable film colony culture device on sale on the market, its Chinese number of patent application is 92222196.0.Its primary structure is that transparent plastics shell of making and the filter membrane that is assemblied in are wherein formed, and cylindrical, import and bleeding point side by side arranged at its top.Plastic casing is bonded by two portions up and down, and filter membrane is bonded in up and down between two portions, and filter membrane is supported by sieve tray, and an outlet is arranged at the bottom, and device is through sterilising treatment, disposable use.Its principle of work is to bleed from bleeding point, makes to form negative pressure in the device, and sample to be checked is in the import suction apparatus, take out negative pressure with sample filtering in outlet then, bacterium is by membrane retention, and resorb substratum to scale is cultivated, and is used for the sterility test of liquid sample.

The problem that prior art exists has: 1. make liquid access to plant complex operation, general sterility test need repeat 20 actions of bleeding, and filters longer duration.2. device can only singlely use, and test sample is representative poor.3.40ml the liquid layer thickness of nutrient solution in incubator has only 3cm.And one of growth conditions of order of succession is that the nutrient solution liquid layer thickness is greater than 7cm when the need order of succession is cultivated simultaneously.

The purpose of this utility model provides a kind of disposable liquid filtering and carries out the device of microbial culture therein, to realize the purpose of quick sample introduction continuous filtration.

For achieving the above object, the solution that the utility model is taked is: design is two four-way structures up and down, and three filtering culturing dish are connected in parallel; Design a controlled path; Internal diameter is made 2.5cm.

Designed two four-way structures owing in the above-mentioned solution, the filtration that makes each duplicate samples makes detection representative, and meets the requirement of sterility test all simultaneously evenly by three filtering culturing dish in ground, has accelerated filtration velocity simultaneously, increases work efficiency.The design controllable passage can utilize Ngatively pressurized sampling, and sample introduction speed is accelerated greatly.Internal diameter is made 2.5cm, make the liquid layer thickness of 40ml nutrient solution in device greater than 7cm, to satisfy the order of succession growth needs.

Below in conjunction with accompanying drawing and two embodiment the utility model is described in further detail:

Fig. 1 is the longitudinal diagram of single filtering culturing dish.

Fig. 2 is the structural representation of the utility model first embodiment.

In longitudinal diagram shown in Figure 1, injection port 1 and outlet 5 are respectively in the top and the bottom of incubator, and controlled path 4 is in a side of incubator, controlled path 4 is by switch 3 controls, shell 6 is made for transparent plastics, and a scale marks 2 is arranged on the wall, and the millipore filtration 7 that is used to collect bacterium is fixed on web plate 8.

Fig. 2 is the structural representation of first embodiment of the utility model.Its using method is: close controlled path, the injection port 1 of three incubators is connected with 10 with four-way structure 9 respectively with outlet 5, four-way opening 11 connects samples, and another four-way opening 12 connects negative pressure systems.When opening negative pressure system, make to form negative pressure in the incubator, the sample mouth enters three incubators respectively simultaneously through four-way opening 11, under suction function, filters through millipore filtration 7, enters in the receiving flask through four-way opening 12.After filtering fully, take off incubator respectively, open controlled path 4 then, injection port 1 is connected nutrient solution, outlet 5 connects negative pressure, and nutrient solution is sucked into scale (40ml), and sealing injection port 1 and outlet 5 are cultivated respectively then.

Second embodiment of the present utility model is that as shown in Figure 1 filtering culturing dish can be used as sterilization filter and uses separately.Method is that injection port 1 is connected sample, and outlet 5 connects negative pressure, opens controlled path 4.Sample is closed controlled path 4 then and is filtered promptly by injection port 1 rapid access to plant, when need once more during sample introduction, only needs to open controlled path 4, repeats above the operation, until finishing filtration.

Claims

1, a kind of disposable millipore filtration incubator, form by shell, base, the web plate of plastics and the millipore filtration that is fixed in the device, it is characterized in that: two four-way devices [9] and [10] were connected in parallel three millipore filtration incubators about it had, and each single incubator all has a controlled path [4].

2, disposable millipore filtration incubator according to claim 1, the liquid layer thickness that it is characterized in that the 40ml nutrient solution is greater than 7cm.

3, disposable millipore filtration incubator according to claim 1 is characterized in that: remove four-way device ［ 9 ］ and ［ 10 ］, and can be separately as sterilizing filter and incubator.