CN109082396A - Bacterium and its application in control of plant disease is quenched in a kind of DSF colony induction signaling molecule - Google Patents
Bacterium and its application in control of plant disease is quenched in a kind of DSF colony induction signaling molecule Download PDFInfo
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Abstract
The invention discloses a kind of DSF colony induction signaling molecules, and bacterium and its application in control of plant disease is quenched.The research of the invention finds that greedy copper bacterium (CupriavidusSp.) growth and breeding can be carried out by sole carbon source, nitrogen source and the energy of DSF, and there is significant degradation effect to the DSF of higher concentration；Simultaneously the present invention also provides the greedy copper bacteria strain HN-2 of a high-efficiency degradation DSF, which was preserved in Guangdong Province's Culture Collection on August 13rd, 2018, and deposit number is GDMCC NO:60432.Greedy copper bacterium HN-2 has significant degrading activity to colony induction signaling molecule in phytopathogen, and it is environmental-friendly, there is huge application potential in the prevention and treatment for relying on the pathogenic phytopathogen that DSF and/or DSF signaling molecule analog mediates, the present invention can reduce abuse of antibiotics problem and pesticide residual contamination problem simultaneously, mitigate environmental pressure, new approaches are provided for biological controlling plant diseases, there is huge application value and wide application prospect.
Description
Technical field
The invention belongs to technical field of biological control.Bacterium is quenched more particularly, to a kind of DSF colony induction signaling molecule
And its application in control of plant disease.
Background technique
DSF(Diffusible Signal Factor) signaling molecule is derived from gram negative pathogenic bacterium, be it is a kind of not
Saturated fatty acid substance.DSF signaling molecule participates in the cell growth of regulating and controlling microbial, the formation of biomembrane and virulence factor
The important biological functions such as generation.The study found that the intervention school-based that DSF signaling molecule mediates not only is widely present in yellow list
Born of the same parents Pseudomonas (Xanthomonas) in bacterium, and germ oligotrophy unit cell (Stenotrophomonas maltophilia), xyllela fastidiosa (Xylellafastidiosa), pseudomonas aeruginosa (Pseudomonas aeruginosa), Burkholderia (Burkholderia) and many marine bacterias in be also found.In addition, a variety of DSF class signals
It has been accredited and has reported like object, such as cis- 2- dodecenoic acid, (2Z, 3Z) -11- methyl -2,5- diene -12- alkanoic acid, cis- 11-
Methyl -2- dodecenoic acid, cis- 2- certain herbaceous plants with big flowers olefin(e) acid, 12- methyl-tetradecanoic acid etc., constitute DSF colony induction signaling molecule man
Race.
Corps diseases frequently can lead to crops and drop in production over a large area, for the transport of agricultural product, storage when disease is serious
And processing but will bring huge loss.Currently, in actual production prevent and treat corps diseases frequently with chemicals.So
And the problems such as will lead to environmental pollution, bacterial drug resistance increase using a large amount of chemicals.In addition to this, it is remained on agricultural product
Chemicals can also jeopardize the health of human body.And colony induction signaling degradation is used as a kind of biological control new strategy, for solution
Certainly the drug resistance problems of microorganism provide new thinking.When carrying out biological control of diseases using this strategy, biocontrol microorganisms will not
Pathogenic bacteria are directly acted on, pathogenic bacteria will not be made to develop drug resistance, meet the requirement of highly effective and safe.
Therefore, it screens the microorganism of energy efficient degradation DSF colony induction signaling molecule and probes into its life to plant disease
Object control efficiency solves the problems, such as that abuse of antibiotics and prevention and treatment DSF and/or DSF signaling molecule are similar to abundant microbial resources
Object mediates pathogenic pathogenic bacteria to have very important realistic meaning.Xanthomonas campestris is carried out both at home and abroad at present
The research of DSF family colony induction signaling molecule quenches mechanism, but it is micro- to degrade about DSF and/or DSF signaling molecule analog
The research of biology is still rarely reported, and DSF degradation bacteria is then more rare.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the defects and deficiency of existing plant disease biological and ecological methods to prevent plant disease, pests, and erosion technology, especially needle
Prevention and treatment to the pathogenic pathogenic bacteria of DSF are relied on provides a kind of new efficient colony induction signaling DSF molecule quenches bacterium, i.e., greedy
Copper bacterium.The research of the invention finds that greedy copper bacterium has significant and quick degradation to colony induction signaling molecule DSF, and have
There is preferable antibiotic resistance, there is prevention and treatment to rely on the huge of the pathogenic bacteria of DSF and/or DSF signaling molecule analog mediation
Potentiality, to solve the problems, such as that pesticide or abuse of antibiotics and bacterial drug resistance increase provide new approaches.
The object of the present invention is to provide the greedy copper bacterium of one plant of degradable DSF signaling molecule (CupriavidusSp.) bacterial strain
HN-2。
Another object of the present invention is to provide greedy copper bacterium in degradation colony induction signaling molecule DSF and the like and prevention and treatment
DSF mediates the application in terms of pathogenic plant disease.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
The present invention provide one plant of degradable DSF signaling molecule greedy copper bacterium (CupriavidusSp.) bacterial strain HN-2, the bacterial strain in
It is preserved on August 13rd, 2018 Guangdong Province's Culture Collection, deposit number is GDMCC NO:60432, and preservation address is
5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100.
The bacterial strain is acquired from Guangzhou, Guangdong Agricultural University Of South China experimental plot rice rhizosphere soil.Through artificial screening point
It is obtained from purifying, by morphological feature, physio-biochemical characteristics and the 16S rRNA Phylogenetic Analysis to the bacterial strain, by the bacterium
Strain be accredited as greedy copper bacterium (CupriavidusSp.).
Bacterial strain HN-2 is Gram-negative bacteria, and bacterium colony is in ecru, and round, flat, surface is smooth opaque, and edge is whole
Together.
Bacterial strain HN-2 is in the shape of a rod under scanning electron microscope, sometimes subsphaeroidal, chain pairs of or in different length.
The condition of culture of the bacterial strain is to be inoculated in culture medium, and optimum temperature is 30 DEG C, and optimal pH 7.2 connects
Kind amount is 1~3 %, and shaking speed is 200 rpm.
The bacterial strain HN-2 reaches 400 μ g/mL or more to the resistance of rifampin, big mould to chloramphenicol, tetracycline, celebrating
The resistance of element, ampicillin and kanamycins is less than 10 μ g/mL.
It has been investigated that the greedy copper bacteria strain HN-2 has significant and quickly degradation to colony induction signaling molecule DSF
Effect is resistant to the DSF at concentrations up to 5 mM, and can believe the quorum sensing DSF that initial concentration is 2 mM in 24 h
Number molecular degradation is complete, has huge application potential in the pathogen harm invention that prevention and treatment DSF is mediated.
Therefore, covet application of the copper bacterium in degradation colony induction signaling molecule DSF and/or DSF signal analog, or making
It makes preparation for dropping the application solved in DSF and/or DSF signal analog in product, it should all be within protection scope of the present invention.
Preferably, the DSF signal analog be include cis- 2- dodecenoic acid, (2Z, 3Z) -11- methyl -2,5- bis-
DSF family including alkene -12- alkanoic acid, cis- 11- methyl -2- dodecenoic acid, cis- 2- certain herbaceous plants with big flowers olefin(e) acid, 12- methyl-tetradecanoic acid
Colony induction signaling molecule.
Greedy copper bacterium mediates the application in pathogenic plant disease in prevention and treatment DSF, or relies on the pathogenic pathogenic bacteria of DSF in preparation
Prevention and treatment preparation in terms of application, also should all be within protection scope of the present invention.
Preferably, in applying described in any of the above-described, the greedy copper bacterium is greedy copper bacteria strain HN-2.
The present invention also provides a kind of methods that prevention and treatment relies on the pathogenic pathogenic fungus diseases of DSF, with the bacteria suspension of greedy copper bacterium
Plant is handled.
Preferably, the dependence DSF pathogenic pathogenic bacteria include but is not limited to that Xanthomonas campestris pv campestris causes a disease
Mutation (Xanthomonas campestris pv. campestris, Xcc) plant black rot caused by XC1, further include
Other DSF and/or DSF signal analogs mediate pathogenic disease.
Preferably, the bacteria suspension of the greedy copper bacterium is the bacteria suspension of bacterial strain HN-2.
In addition, a kind of degradation of the degradable colony induction signaling molecule DSF containing bacterial strain HN-2 and/or its bacteria suspension
The biological prevention and control agent and its correlation for the pathogenic bacteria that agent and a kind of dependence DSF containing bacterial strain HN-2/ or its bacteria suspension cause a disease are answered
With also all should be in the scope of the present invention.
Preferably, the degradation agent and biological prevention and control agent are to be prepared by bacterial strain HN-2 through the resulting bacteria suspension of everfermentation.
Preferably, the bacteria suspension the preparation method comprises the following steps: bacterial strain HN-2 is inoculated in LB solid medium tablets respectively, 30
It DEG C being incubated overnight, picking single colonie, is seeded to LB liquid medium overnight incubation according to 1 ~ 3 % of inoculum concentration, initial pH is 7.2,
Shaking speed is 200 rpm, and bacterial strain HN-2 bacterium solution is centrifuged 10 min in 4000 g, abandons supernatant, then laid equal stress on aseptic water washing
It is outstanding, obtain bacteria suspension.
It is highly preferred that the optimum medium used when preparing the bacteria suspension of greedy copper bacterium is MM culture medium, formula includes
4.5 g/L KH2PO4、2.0 g/L (NH4)2SO4、0.005 g/L FeSO4、10.5 g/L K2HPO4、0.2 g/L MgSO4·
7 H2O、0.01 g/L CaCl2、0.002 g/L MnCl2。
Furthermore it is preferred that the pH of control system is 7.0~7.5 in above-mentioned application, temperature is 28~32 DEG C, and effect is more
It is good.
It is highly preferred that the pH of control system is 7.2, temperature is 30 DEG C.
Preferably, concentration≤5 mMs of the control DSF in system.
The invention has the following advantages:
The research of the invention finds that greedy copper bacterium (CupriavidusSp.) have to colony induction signaling molecule DSF significant and quick
There is prevention and treatment to rely on the pathogenic pathogen great potential of DSF, ask solution pesticide or abuse of antibiotics and drug resistance for degradation
Topic has realistic meaning.
The present invention screens to have obtained one plant of greedy copper bacteria strain HN-2, has DSF high degrading activity, using DSF as uniquely
Carbon source, nitrogen source and the energy carry out growth and breeding, and greedy copper bacterium HN-2 is resistant to the DSF at concentrations up to 5 mM, and can in 24 h
It is complete the DSF of 2 mM to degrade.Greedy copper bacterium HN-2 and Xanthomonas campestris XC1 co-inoculation of the invention is compared to independent inoculation
The black rot degree of disease that dialogue radish fleshy root causes when XC1 is substantially reduced, to reach the effect to the prevention and treatment of white radishes black rot
Fruit.
In addition, the strain isolation is from rice rhizosphere soil, it is environmentally friendly, and adaptability is preferable.Therefore, the present invention provides
Greedy copper bacterium HN-2 have to the higher degrading activity of colony induction signaling molecule in phytopathogen, and it is environmental-friendly, according to
There is huge application potential in the prevention and treatment for the pathogenic phytopathogen for relying DSF and/or DSF signaling molecule analog to mediate, together
When the present invention can reduce abuse of antibiotics problem and pesticide residual contamination problem, mitigate environmental pressure, be biological control plant
Disease provides new approaches.
Detailed description of the invention
Fig. 1 is the colonial morphology figure of bacterial strain HN-2 of the invention on nutrient agar.
Fig. 2 is the scanning electron microscope (SEM) photograph of bacterial strain HN-2 of the invention.
Fig. 3 is the Phylogenetic analysis figure of bacterial strain HN-2 of the invention.
Fig. 4 is the growing state figure of bacterial strain HN-2 of the invention in different antibiotic.
Fig. 5 be bacterial strain HN-2 of the invention using DSF as sole carbon source when growth curve and degradation curve figure.
Fig. 6 is that bacterial strain HN-2 of the invention is individually inoculated with and with xanthomonas campestris co-inoculation in white radishes fleshy root
It is sliced the incidence that white radishes fleshy root is sliced after 48 h.
Fig. 7 is that bacterial strain HN-2 of the invention is individually inoculated with and with xanthomonas campestris co-inoculation in potato slices 48
The incidence of potato slices after h.
Specific embodiment
The present invention is further illustrated below in conjunction with Figure of description and specific embodiment, but embodiment is not to the present invention
It limits in any form.Unless stated otherwise, the present invention uses reagent, method and apparatus routinely try for the art
Agent, method and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
The separation of 1 bacterial strain HN-2 of embodiment is identified
1, the separation, screening of bacterial strain
(1) for soil collection from the soil of Guangzhou, Guangdong Agricultural University Of South China experimental field rice rhizosphere, soil is in yellowish-brown.
(2) bacterial strain isolates and purifies
MM culture medium is prepared, dispenses into 250 mL triangular flasks, the MM culture medium of 50 mL, high-pressure sterilizing pot is dispensed in each bottle
Sterilizing is added DSF mother liquor after its cooling in superclean bench, makes the 50 μm/L of ultimate density of DSF in culture medium, together
When 5 g of soil sample is added in the medium.30 DEG C in shaking table, after being cultivated 7 days under the conditions of 200 rpm, turn by 10% inoculum concentration
It is connected in the MM culture medium that second batch DSF concentration is 100 μm/L.After the same terms culture 7 days, then by 10% inoculum concentration switching
In the MM culture medium for being 200 μm/L to DSF concentration, continue culture 7 days.And so on, it is continuously increased the dense of DSF in culture medium
Degree.
Isolating and purifying for bacterial strain is carried out using the method for dilution, plate coating scribing line later.1 mL end MM culture medium is taken to send out
It is 10 that zymotic fluid, which is diluted to concentration gradient with sterile water,-1、10-2、10-3、10-4、10-5、10-6、10-7、10-8Fermentation liquid, so
The fermentation liquid for drawing the 100 each concentration gradients of μ L respectively afterwards is spread evenly across on LB solid plate, culture in 30 DEG C of incubators.
It then is in the single colonie of different colonial morphologies, culture purified of crossing repeatedly on LB solid plate, until separation in picking plate
Single colonie out.Single colonie is seeded to LB liquid medium culture and obtains bacterium solution, condition of culture is 30 DEG C, 200 rpm.By bacterium solution
After being mixed in a certain proportion with glycerol, it is put into -80 DEG C of preservations.
MM culture medium prescription are as follows: KH2PO4, 4.5 g/L；(NH4)2SO4, 2.0 g/L；FeSO4, 0.005 g/L；K2HPO4,
10.5 g/L；MgSO4·7 H2O, 0.2 g/L；CaCl2, 0.01 g/L；MnCl2, 0.002 g/L.
(3) screening of bacterial strain
Isolated bacterial strain from soil sample is sieved using the MM basal medium using the DSF of 5 mM as sole carbon source
Choosing.After bacterial strain after isolating and purifying is activated, picking single colonie is seeded to 50 bases mL MM that DSF concentration is 5 mM and trains
It supports in base, cultivates 48 h under conditions of 30 DEG C, 200 rpm, carry out the extraction of DSF and measure DSF residual volume using HPLC,
Degradation rate (%)=(1-A1/A0) × 100%, A1For DSF residual concentration after bacterial strain processing, A0It is dense for the DSF residual after control treatment
Degree.
The extracting method of DSF: each sample takes 5 mL into 15 mL centrifuge tubes, and 4000 g are centrifuged 5 min, takes supernatant
It is transferred in 50 mL separatory funnels, 5 mL ethyl acetate is added into separatory funnel, shake up, acutely shake 3 min, stand, point
Layer, discards lower layer's solution into 15 mL centrifuge tubes, upper liquid is filled into 50 mL round-bottomed flasks through funnel, and funnel spreads filter paper
And 5 g anhydrous sodium sulfates are housed.Lower layer's solution extracts 1 time again according to the above method.Filtrate is incorporated to round-bottomed flask, 50 DEG C of constant temperature concentrations
It is evaporated, with 3 washing round-bottomed flasks of chromatography methanol point, is settled to 2 mL, through 0.45 μM of organic membrane filtration to sample injection bottle, makes
Its residual volume is measured with HPLC method.HPLC measures DSF residual volume condition: C18Reverse chromatograms column, flow velocity are 1 mL/min, and column temperature is
35 DEG C, mobile phase is methanol: water=80:20(ν:ν), Detection wavelength is 210 nm, 20 μ L of sample volume.
Screening obtains one plant of highest bacterial strain of DSF degradation rate, is named as HN-2.
2, the identification of bacterial strain
(1) colony morphology characteristic of bacterial strain HN-2
Bacterial strain HN-2 bacterium colony is in ecru, and round, flat, surface is smooth opaque, neat in edge (Fig. 1).Under scanning electron microscope
Thallus is in the shape of a rod, sometimes subsphaeroidal, chain (Fig. 2) pairs of or in different length.
(2) the 16S rRNA identification of bacterial strain HN-2
The 16S rRNA gene of bacterial strain HN-2 is cloned, is sequenced, Blast comparison is then carried out in GenBank.As a result it shows
Show, with its sequence similarity reach 99% be greedy copper bacterium (CupriavidusSp.), systematic evolution tree is as shown in Figure 3.
Therefore, bacterial strain HN-2 identified through morphological feature and 16S rRNA Molecular Identification be greedy copper bacterium (Cupriavidus
Sp. Guangdong Province's Culture Collection, deposit number), and on August 13rd, 2018 are preserved in are as follows: GDMCC NO:60432,
Preservation address is 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100.
Embodiment 2 covets the antibiotics sensitivity analysis of copper bacterium HN-2
In order to preferably study the Biocontrol Potential for the bacterial strain HN-2 that embodiment 1 obtains, we are to the bacterial strain antibiotic sensitive
Property is studied.
As a result as shown in figure 4, the bacterial strain reaches 400 μ g/mL or more to the resistance of rifampin (RIF), to chloramphenicol
(CM), the resistance of tetracycline (TC), gentamicin (GEN), ampicillin (AMP) and kanamycins (KAN) is less than 10 μ g/
mL。
The results show that bacterial strain HN-2 shows higher drug resistance to rifampin, and to big to chloramphenicol, tetracycline, celebrating
The resistance of mycin, ampicillin and kanamycins is lower, this result is conducive in follow-up study choose suitable antibiotic work
For reference.
Embodiment 3 covets the measurement of copper bacterium HN-2 growth and DSF degradation relation curve
1, picking bacterial strain HN-2 single colonie is inoculated in LB culture medium preculture to logarithmic phase, item of the gained bacterium solution in 4000 rpm
After being centrifuged 5 min under part, liquid is discarded supernatant, thallus is rinsed and is resuspended with 0.9% sterile saline, as seed suspension, then
It is seeded in 50 mL MM basal mediums with 1~3% inoculum concentration, and adds DSF mother liquor, make 2 mM of its ultimate density,
It 30 DEG C, cultivates under the conditions of 200 rpm, timing sampling.The sample of different time points is acquired, spectrophotometric determination OD is carried out600Value
Indicate the growing state of bacterial strain HN-2, the residual quantity that HPLC measures DSF indicates bacterial strain HN-2 to the degradation situation of DSF.
2, the growth of bacterial strain HN-2 and DSF degradation relational graph are as shown in Figure 5 when using DSF as sole carbon source.As shown,
The degradation of DSF is positively correlated with strain growth, and in the presence of DSF, strain growth does not have demurrage, quickly enters in 6~12h
Logarithmic phase is grown, most fast, the strain culturing to 24 h of DSF degradation at this time, DSF degradation is completely.In control in 24 h DSF natural drop
Solution rate is less than 20%.
The result shows that greedy copper bacterium HN-2 has significant and quick degradation to DSF, in the pathogenic bacteria that prevention and treatment DSF is mediated
Harm aspect has huge application potential.
Embodiment 4 is coveted copper bacterium HN-2 and is studied the biocontrol effect of white radishes black rot
1, it is black to white radishes to the pathogenic pathogenic bacteria of DSF are relied on to study greedy copper bacterium HN-2 by taking Xanthomonas campestris XC1 as an example for the present embodiment
The biocontrol effect of maize ear rot.
By bacterial strain HN-2 and the pathogenic pathogenic bacteria Xanthomonas campestris XC1 of DSF is relied on, it is flat to be inoculated in LB solid medium respectively
Plate, 30 DEG C are incubated overnight.Single colonie is picked them separately, LB liquid medium overnight incubation is seeded to, by bacterial strain HN-2 bacterium solution and XC1
Bacterium solution is centrifuged 10 min in 4000 g, abandons supernatant, then with aseptic water washing and be resuspended, obtains bacteria suspension.
Bacterial strain HN-2 bacteria suspension and strain X C1 bacteria suspension are uniformly mixed so as to obtain mixed bacteria liquid.Mixed bacteria liquid, XC1, HN-2 bacterium solution
Equal OD600=0.2, four groups of HN-2+ sterile water, HN-2+XC1, sterile water, XC1+ sterile water experiments are respectively set.By white radishes meat
Matter root is clean wash with distilled water, is sliced after appearance is dry.The crosscutting circle for obtaining about 0.2~0.3 cm of thickness of fleshy root
Piece is respectively put into culture dish (built-in with the cotton of sterile water infiltration).Each experimental group takes 100 μ L of bacterium solution, drips in white trailing plants
Foretell on fleshy root slice, is smeared and smoothened with spreading rod.Then the white radishes fleshy root being inoculated with slice is put into 28 DEG C
Culture in insulating box.Routine observation simultaneously records incidence.
2, bacterial strain HN-2 is as shown in Figure 6 to the biocontrol effect of white radishes black rot.The result shows that bacterial strain HN-2 and sarson
Black rot degree of disease caused by dialogue radish fleshy root is sliced when Xanthomonas campestris XC1 co-inoculation is more individually inoculated with XC1 obviously subtracts
Gently.According to the experimental results, bacterial strain HN-2 has obviously white radishes black rot caused by xanthomonas campestris XC1
Biocontrol effect.
Embodiment 5 is coveted copper bacterium HN-2 and is studied the biocontrol effect of potato black rot
1, it is black to potato to the pathogenic pathogenic bacteria of DSF are relied on to study greedy copper bacterium HN-2 by taking Xanthomonas campestris XC1 as an example for the present embodiment
The biocontrol effect of maize ear rot.
The pathogenic pathogenic bacteria Xanthomonas campestris XC1 of bacterial strain HN-2 and dependence DSF is lined into LB solid medium tablets respectively,
30 DEG C are incubated overnight.Single colonie is picked them separately, LB liquid medium overnight incubation is seeded to, by bacterial strain HN-2 bacterium solution and XC1 bacterium
Liquid is centrifuged 10 min in 4000 g, abandons supernatant, then with aseptic water washing and be resuspended, obtains bacteria suspension.
The suspension of bacterial strain HN-2 is uniformly mixed to obtain mixed bacteria liquid with the suspension of strain X C1.Gained bacterium solution is finally dense
Degree is OD600=2.The chemical pesticide agricultural streptomycin of black rot will be usually used in preventing and treating as positive control, by 72% agriculture when experiment
It is used with 3000 times of streptomysin soluble powder sterile water dissolved dilution.Sterile water, HN-2+ sterile water, HN-2+ is respectively set
Five groups of XC1, XC1+ sterile water, XC-1+ agricultural streptomycin experiments.By potato tubers it is clean wash with distilled water after be sliced
Processing.The crosscutting Potato Tuber Slices for obtaining about 0.2~0.3 cm of thickness of potato tubers, are gently washed by rubbing with the hands three times with distilled water,
It to remove the starch contained in stem tuber, is neatly put into the ceramic whiteware disk for being lined with filter paper, next step experiment is carried out after dry tack free.
100 μ L bacteria suspensions are taken with liquid-transfering gun, is dripped on each thin slice, is smeared and smoothened with spreading rod.Potato will be then placed with
Ceramic whiteware disk is put into 28 DEG C of insulating boxs and cultivates.Regular section carries out observing and recording lower incidence.
2, bacterial strain HN-2 is as shown in Figure 7 to the biocontrol effect of potato black rot.As shown, sterile water and HN-2+ without
In bacterium water experimental group, potato does not fall ill, illustrates sterile water and bacterial strain HN-2 to potato no pathogenicity.XC1+ sterile water is real
The potato rot of group is tested, seriously locates to be lividity, but no foul smell, only one dry vegetalbe taste, be distinguished with soft rot；XC-1+
Agricultural streptomycin experimental group potato does not fall ill, and HN-2+XC1 experimental group potato is only slight morbidity.The result shows that biocontrol microorganisms
HN-2 has apparent control efficiency and close with agricultural streptomycin control efficiency to black rot.
To sum up the result shows that, growth and breeding can be carried out as sole carbon source, nitrogen source and the energy using DSF by coveting copper bacterium HN-2,
And it is resistant to the DSF at concentrations up to 5 mM, and the DSF of 2 mM can degrade completely in 24 h, have to the DSF of high concentration
There is stable and efficient degrading activity.By greedy copper bacterium HN-2 and xanthomonas campestris XC1 co-inoculation in white radishes or potato
When, it is substantially reduced to black rot degree of disease caused by white radishes or potato when compared with independent inoculation XC1, illustrate that the bacterial strain can answer
For relying in the pathogenic pathogenic bacteria biological control of DSF and/or DSF signaling molecule analog, it is possible to reduce chemical pesticide makes
With mitigating environmental pressure, have broad application prospects and development potentiality.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (9)
1. one plant of degradable DSF colony induction signaling molecule greedy copper bacterium (CupriavidusSp.) bacterial strain HN-2, feature exist
In the bacterial strain was preserved in Guangdong Province's Culture Collection on August 13rd, 2018, and deposit number is GDMCC NO:
60432。
2. application of the greedy copper bacterium in degradation colony induction signaling molecule DSF and/or DSF signal analog, or degrade in preparation
Application in the product of DSF and/or DSF signal analog.
3. greedy copper bacterium mediates the application in pathogenic plant disease in prevention and treatment DSF, or relies on the pathogenic pathogenic bacteria of DSF in preparation
Application in terms of control agent.
4. application according to claim 2 or 3, which is characterized in that the greedy copper bacterium is to covet copper bacterium bacterium described in claim 1
Strain HN-2.
5. a kind of method that prevention and treatment relies on the pathogenic pathogenic fungus diseases of DSF, which is characterized in that with greedy copper bacterium and/or greedy copper bacterium
Bacteria suspension handles plant.
6. according to the method described in claim 5, it is characterized in that, preparing the culture medium of the bacteria suspension of greedy copper bacterium as MM culture
Base, formula include 4.5 g/L KH2PO4、2.0 g/L (NH4)2SO4、0.005 g/L FeSO4、10.5 g/L K2HPO4、
0.2 g/L MgSO4·7 H2O、0.01 g/L CaCl2With 0.002 g/L MnCl2。
7. a kind of degradation bacterial agent of degradable colony induction signaling molecule DSF, which is characterized in that contain greedy copper bacterium and/or its bacterium
Suspension.
8. a kind of biocontrol agent for the pathogenic bacteria caused a disease dependent on DSF, which is characterized in that contain greedy copper bacterium HN-2 and/or its bacterium
Suspension.
9. according to the microbial inoculum of claim 7 or 8, which is characterized in that containing described in claim 1 covet copper bacterium HN-2 and/or its
Bacteria suspension.
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