CN114891630B

CN114891630B - System and method for detecting vitality of cerasus campanulata pollen

Info

Publication number: CN114891630B
Application number: CN202210446973.2A
Authority: CN
Inventors: 李蒙; 伊贤贵; 段一凡; 汪小飞; 王贤荣
Original assignee: Nanjing Forestry University
Current assignee: Nanjing Forestry University
Priority date: 2022-04-26
Filing date: 2022-04-26
Publication date: 2023-03-21
Anticipated expiration: 2042-04-26
Also published as: CN114891630A

Abstract

The invention discloses a primrose florescence vitality detection system which comprises a detection table, a bearing rack, brackets, a mixer, storage tanks, trays, a culture dish, a microscope and a driving circuit, wherein the detection table and the brackets are connected with the upper end surface of the detection table, two brackets are symmetrically distributed on two sides of the detection table, one bracket is connected with at least two storage tanks and one mixer, the other bracket is provided with at least one microscope, the trays are embedded in the cavity of the detection table, the culture dish is connected with the upper end surface of the trays, and the driving circuit is embedded in the bearing rack. The detection method comprises the steps of system setting, detection presetting, cultivation detection and detection operation. On one hand, the invention improves the integration and modularization degree of the structure, thereby greatly improving the flexibility of the use of the detection equipment and the convenience of maintenance operation; the other side has high automation degree of detection operation and high operation precision, and can effectively improve the efficiency of pollen activity detection operation, thereby greatly improving the precision of the pollen activity detection operation.

Description

System and method for detecting vitality of cerasus campanulata pollen

Technical Field

The invention relates to a system and a method for detecting the vitality of cerasus campanulata pollen, belonging to the technical field of plant breeding equipment.

Background

At present, in the works of cerasus campanulata hybridization, breeding and the like, the pollen germination capacity of plants is often required to be detected, so that reliable basis is provided for the works of hybridization breeding and the like, aiming at the requirement, various pollen activity detection equipment and technologies are developed currently, for example, equipment such as a cerasus campanulata pollen storage and in-vitro culture viability detection system and method with the patent application number of 2021106656743", a major camellia pollen viability measuring device with the patent application number of 2017205906045 and the like can meet the requirements of pollen activity detection operation, but the equipment structure is complex and relatively fixed in different degrees in actual use, the equipment structure cannot be flexibly adjusted according to the use requirement, so that the pollen activity detection equipment is poor in use flexibility and universality, high in operation difficulty and relatively high in equipment maintenance operation cost, in addition, in the detection process, the detection of multiple groups of samples cannot be simultaneously realized, and the detection of the multiple groups of samples cannot be effectively maintained under the same induction environment for detection, so that the data of the detection operation have larger deviation, and the effective detection efficiency can be relatively low.

Disclosure of Invention

Therefore, in order to solve the problem, the development of a primula sikkmensis pollen activity detection system and a method thereof is urgently needed to meet the needs of practical use. Disclosure of Invention

In order to solve the defects in the prior art, the invention provides a closterium-caerulea pollen activity detection system and a method thereof.

A primula sikkmensis flower powder activity detection system comprises a detection platform, a bearing frame, brackets, a mixer, a feeding port, storage tanks, a peristaltic pump, a tray, a rotary driving mechanism, a culture dish, a microscope and a driving circuit, wherein the bearing frame is of a rectangular frame structure with a cross section, the detection platform and the brackets are connected with the upper end surface of the bearing frame, at least one detection platform is provided, the axis of the detection platform is vertically distributed with the upper end surface of the bearing frame, two brackets are symmetrically distributed on two sides of the detection platform, the brackets are in a shape like 20866and are connected with at least two storage tanks and one mixer, the storage tanks are mutually connected in parallel and are communicated with the peristaltic pump through a guide pipe, the peristaltic pump is connected with the brackets and is communicated with the mixer through the guide pipe, the mixer is communicated with the feeding port through the peristaltic pump, and at least one microscope is arranged on the other bracket, the microscope and the feeding port are both positioned above the detection table, the distance between the microscope and the center of the detection table is not more than 30% of the width of the detection table, the axes of the microscope and the feeding port are intersected with the upper end face of the detection table, the detection table is of a cavity structure with a rectangular axial section, guide ports are arranged on the side surface positions corresponding to the bracket, operation ports are arranged on the upper end face of the position corresponding to the bracket, the tray is of a disc structure coaxially distributed with the detection table and is embedded in the cavity of the detection table and connected with the bottom of the cavity of the detection table through a rotary driving mechanism, at least two culture dishes are uniformly distributed around the axis of the tray and connected with the upper end face of the tray, and when the culture dishes are positioned at the operation ports, the culture dishes are coaxially distributed with the operation ports and are positioned outside the cavity of the detection table through the operation ports and the guide ports, and the driving circuit is embedded in the bearing frame and is electrically connected with the detection table, the peristaltic pump and the rotary driving mechanism respectively.

Furthermore, examine test table for any one in rectangle and circular cavity structure, the operation mouthful position is examined test table up end and is established transparent cover, passes through spout sliding connection between transparent cover and operation mouthful lateral wall, examine test table inside and establish temperature and humidity sensor, far infrared irradiation device, ultraviolet irradiation device, and a temperature and humidity sensor, a far infrared irradiation device and an ultraviolet irradiation device constitute an adjusting group, and the adjusting group quantity is no less than two, each adjusting group symmetry distributes in two operation mouthful central point connecting wire both sides to encircle and examine test table axis distribution, just temperature and humidity sensor, far infrared irradiation device, ultraviolet irradiation device all are connected with examining test table top, and its axis intersects and the vertical distribution with the tray up end, temperature and humidity sensor, far infrared irradiation device, ultraviolet irradiation device all are connected with drive circuit electrical connection.

Furthermore, the transparent cover plate is of an L-shaped groove structure in cross section, and when the transparent cover plate and the operation port are coaxially distributed, the transparent cover plate is respectively coated outside the operation port and the guide port and forms a closed cavity structure with the detection platform.

Further, examine test table cavity in establish the baffle, the baffle respectively with examine test table up end and be connected and the vertical distribution to along two operation mouth central point connecting wire direction distributions, will examine test table interior division into two and cultivate the chamber through the baffle, and each is cultivated the test table bottom that the chamber corresponds and establishes the access hole.

Furthermore, the tray is of a U-shaped frame structure with a cross section, the bottom of the tray is of a grating plate structure, a plurality of elastic supporting blocks are uniformly distributed on the upper end surface of the tray around the axis of the tray, the height of each elastic supporting block is not more than 60% of the depth of the tray, a sucking disc is arranged on the upper end surface of each elastic supporting block and is connected with the lower end surface of each culture dish through the sucking disc, the lower end surface of each culture dish is connected with at least three elastic supporting blocks uniformly distributed around the axis of the culture dish, and the culture dishes are distributed in parallel with the upper end surface of the tray.

Further, the bracket include cross arm, stand, slider, the cross arm both ends pass through slider and stand side surface sliding connection and vertical distribution, slider rear end face and stand side surface pass through spout sliding connection, and the spout inlays in the stand side surface and with stand axis parallel distribution, the slider front end face passes through ratchet and is articulated with the cross arm terminal surface, and the terminal surface is 0-90 contained angle with the stand axis under the cross arm, wherein blender, storage tank, peristaltic pump and microscope all are connected with the cross arm, wherein the blender is connected with the terminal surface under the cross arm.

Furthermore, the driving circuit is a circuit system based on any one of a DSP chip and an FPGA chip.

A detection method of a primula sikkmensis hook pollen activity detection system comprises the following steps:

s1, system setting, namely firstly assembling a detection table, a bearing rack, a bracket, a mixer, a feeding port, a storage tank, a peristaltic pump, a tray, a rotary driving mechanism, a microscope and a driving circuit to obtain detection equipment, then installing and positioning the detection equipment to a specified working position through the bearing rack, connecting the driving circuit with an external power supply system, and sterilizing and storing each culture dish to obtain finished product detection equipment;

s2, detection presetting, namely firstly, respectively adding nutrient media and pollen for pollen cultivation into each storage tank, then, installing each culture dish for detection operation on a bracket through an operation opening corresponding to a feeding opening, then, driving a peristaltic pump and a mixer to operate to mix the nutrient media and the pollen in each storage tank, finally, adding the mixture into the culture dish through a feeding opening, finally, driving a tray to rotate by a rotary driving mechanism, rotating the culture dish with the mixture addition completed into a cultivation cavity in a detection platform, then, installing a new culture dish on the bracket at the operation opening corresponding to the feeding opening again for mixture addition, and conveying the new culture dish into the cultivation cavity until parts meeting the detection requirement are prepared, and embedding each culture dish with the mixture into the cultivation cavity;

s3, cultivation detection is carried out, after the step S2 is completed, the temperature and humidity sensor, the far infrared irradiation device and the ultraviolet irradiation device in the cultivation cavity are driven to operate, and on one hand, the mixture in the culture dish is subjected to infrared heating through the far infrared irradiation device; on the other hand, the mixture is subjected to ultraviolet radiation induction through an ultraviolet radiation device; promoting pollen germination in the mixture in the culture dish, and simultaneously detecting the environmental temperature and humidity in the culture cavity by a temperature and humidity sensor in the running process of the far infrared irradiation device and the ultraviolet irradiation device to realize the induced germination of the pollen;

and S4, detecting, namely driving the rotary driving mechanism to operate after the operation of the step S3 is completed, conveying the culture solution in the cultivation cavity after the induction germination is completed to the operation opening of the detection platform corresponding to the microscope from the cultivation cavity, and then carrying out pollen germination state detection and counting by the microscope.

Compared with the traditional pollen activity detection equipment, the pollen activity detection equipment has the advantages that on one hand, the integration and modularization degree of the structure is improved, so that the use flexibility and the maintenance operation convenience of the detection equipment are greatly improved, and the detection requirements of various types of pollen are effectively met; on the other hand, during operation, the detection operation has high automation degree and high operation precision, and the efficiency of the pollen activity detection operation can be effectively improved, so that the precision of the pollen activity detection operation is greatly improved.

Drawings

The invention is described in detail below with reference to the drawings and the detailed description;

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the structure of the inspection table;

FIG. 3 is a schematic view of a tray structure;

FIG. 4 is a schematic view of a bracket structure;

FIG. 5 is a flow chart illustrating a method of using the present invention.

Detailed Description

In order to facilitate the implementation of the technical means, creation features, achievement of the purpose and the efficacy of the invention, the invention is further described below with reference to specific embodiments.

As shown in fig. 1-4, a primula sikkmensis hook pollen activity detection system comprises a detection table 1, a bearing rack 2, brackets 3, a mixer 4, a feeding port 5, storage tanks 6, a peristaltic pump 7, a tray 8, a rotary driving mechanism 9, a culture dish 10, a microscope 11 and a driving circuit 12, wherein the bearing rack 2 has a cross section in a rectangular frame structure, the detection table 1 and the brackets 3 are both connected with the upper end surface of the bearing rack 2, at least one detection table 1 is vertically distributed with the axis thereof and the upper end surface of the bearing rack 2, two brackets 3 are symmetrically distributed on two sides of the detection table 1, the brackets 3 are in a shape of 20866, one bracket 3 is connected with at least two storage tanks 6 and one mixer 4, the storage tanks 6 are mutually connected in parallel and are communicated with the peristaltic pump 7 through a guide pipe, the pump 7 is connected with the bracket 3 and is communicated with the mixer 4 through the guide pipe, the mixer 4 is communicated with a feeding port 5 through a peristaltic pump 7, at least one microscope 11 is arranged on another bracket 3, the microscope 11 and the feeding port 5 are both positioned above the detection platform 1, the distance between the microscope 11 and the center of the detection platform 1 is not more than 30% of the width of the detection platform 1, the axes of the microscope 11 and the feeding port 5 are both intersected with the upper end surface of the detection platform 1, the detection platform 1 is of a cavity structure with a rectangular axial section, the side surface positions corresponding to the bracket 3 are both provided with guide ports 13, the upper end surface of the position corresponding to the bracket 3 is provided with an operation port 14, the tray 8 is of a disc structure which is coaxially distributed with the detection platform 1, is embedded in the cavity of the detection platform 1 and is connected with the bottom of the cavity of the detection platform 1 through a rotary driving mechanism 9, at least two culture dishes 10 are uniformly distributed around the axis of the tray 8 and are connected with the upper end surface of the tray 8, and when the culture dishes 10 are positioned at the operation port 14, the culture dishes 10 are coaxially distributed with the operation port 14, and is located outside the cavity of the detection table 1 through an operation port 14 and a guide port 13, and a driving circuit 12 is embedded in the bearing frame 2 and is respectively and electrically connected with the detection table 1, the peristaltic pump 7 and the rotary driving mechanism 9.

It is emphasized that, the detection platform 1 is any one of rectangular and circular cavity structures, the upper end face of the detection platform 1 at the position of the operation port 14 is provided with a transparent cover plate 15, the transparent cover plate 15 is in sliding connection with the side wall of the operation port 14 through a sliding chute 25, the detection platform 1 is internally provided with a temperature and humidity sensor 16, a far infrared irradiation device 17 and an ultraviolet irradiation device 18, the temperature and humidity sensor 16, the far infrared irradiation device 17 and the ultraviolet irradiation device 18 form an adjusting group, the number of the adjusting groups is not less than two, the adjusting groups are symmetrically distributed on two sides of a connecting line of central points of the two operation ports 14 and distributed around the axis of the detection platform 1, the temperature and humidity sensor 16, the far infrared irradiation device 17 and the ultraviolet irradiation device 18 are all connected with the top of the detection platform 1, the axis of the temperature and humidity sensor 16, the far infrared irradiation device 17 and the ultraviolet irradiation device 18 are intersected and vertically distributed with the upper end face of the tray 8, and the temperature and humidity sensor 16, the far infrared irradiation device 17 and the ultraviolet irradiation device 18 are all electrically connected with the driving circuit 12.

Preferably, the cross section of the transparent cover plate 15 is in an L-shaped groove structure, and when the transparent cover plate 15 and the operation port 14 are coaxially distributed, the transparent cover plate 15 covers the operation port 14 and the guide port 13 respectively and forms a closed cavity structure with the detection table 1.

In addition, examine test table 1 cavity in establish baffle 19, baffle 19 respectively with examine test table 1 up end and be connected and vertical distribution to along two 14 central points of operation mouth connecting line directions distribution, will examine test table interior division into two and cultivate chamber 20 through baffle 19, and each cultivate the inspection hatchway 21 of examining test table 1 bottom that chamber 20 corresponds.

In this embodiment, the tray 8 has a frame structure with a cross section shaped like a "u", the bottom of the tray 8 is a grid plate, the upper end surface of the tray is provided with a plurality of elastic support blocks 22 uniformly distributed around the axis of the tray 8, the height of each elastic support block 22 is not more than 60% of the depth of the tray 8, the upper end surface of the tray is provided with a suction cup 23 and is connected with the lower end surface of each culture dish 10 through the suction cup 23, the lower end surface of each culture dish 10 is connected with at least three elastic support blocks 22 uniformly distributed around the axis of the culture dish 10, and the culture dishes 10 and the upper end surface of the tray 8 are distributed in parallel.

In this embodiment, the bracket 3 includes a cross arm 31, a vertical column 32, and a sliding block 33, two ends of the cross arm 31 are slidably connected to and vertically distributed on a side surface of the vertical column 32 through the sliding block 33, a rear end surface of the sliding block 33 is slidably connected to a side surface of the vertical column 32 through a sliding groove 25, the sliding groove 25 is embedded in the side surface of the vertical column 32 and is distributed parallel to an axis of the vertical column 32, a front end surface of the sliding block 33 is hinged to an end surface of the cross arm 31 through a ratchet mechanism 34, and a lower end surface of the cross arm 31 forms an included angle of 0 ° to 90 ° with the axis of the vertical column 32, wherein the mixer 4, the storage tank 6, the peristaltic pump 7, and the microscope 11 are all connected to the cross arm 31, and the mixer 4 is connected to the lower end surface of the cross arm 31.

In this embodiment, the driving circuit 12 is a circuit system based on any one of a DSP chip and an FPGA chip.

Further, the microscope 11 is any one of an optical microscope and an electron microscope.

Preferably, a sterilization cabinet 24 is arranged in the bearing rack 2, the sterilization cabinet 24 is electrically connected with the driving circuit 12, and the culture dishes 10 in standby state are stored in the sterilization cabinet 24.

As shown in fig. 5, a detection method of a primula sikkmensis pollen viability detection system comprises the following steps:

and S4, detecting, namely driving the rotary driving mechanism to run after the operation of the step S3 is completed, conveying the culture solution in the cultivation cavity after the induction germination is completed to an operation port of the detection table corresponding to the microscope from the cultivation cavity, and then detecting and counting the germination state of the pollen by the microscope.

In this embodiment, in practical operation, when each culture dish is operated, the three steps of presetting, cultivation and detection of the culture dish can be continuously performed in a circulating manner, and when the continuous operation in a circulating manner is performed, the operating efficiency of the peristaltic pump and the rotary driving mechanism is adjusted, and the operating power of the far infrared irradiation device and the operating power of the ultraviolet irradiation device are adjusted, so that the culture dish is mixed and filled for the same time as the cultivation time of the cultivation chamber on the culture dish, and the continuous circulating detection of a plurality of detection samples is realized.

The foregoing shows and describes the general principles and features of the present invention, together with the advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, and such changes and modifications are within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a cloaca sakura pollen activity detecting system which characterized in that: the primula sikkmensis hook pollen activity detection system comprises a detection table, a bearing rack, brackets, a mixer, a feeding port, storage tanks, a peristaltic pump, a tray, a rotary driving mechanism, a culture dish, a microscope and a driving circuit, wherein the bearing rack is of a rectangular frame structure with a cross section, the detection table and the brackets are connected with the upper end surface of the bearing rack, at least one detection table is provided, the axis of the detection table is vertically distributed with the upper end surface of the bearing rack, two brackets are symmetrically distributed on two sides of the detection table, the brackets are in a shape like 20866, one bracket is connected with at least two storage tanks and one mixer, the storage tanks are mutually connected in parallel and are communicated with the peristaltic pump through a guide pipe, the peristaltic pump is connected with the brackets and is communicated with the mixer through the guide pipe, the mixer is communicated with the feeding port through the peristaltic pump, and at least one microscope is arranged on the other bracket, the microscope and the feeding port are both positioned above the detection table, the distance between the microscope and the feeding port and the center of the detection table is not more than 30% of the width of the detection table, the axes of the microscope and the feeding port are intersected with the upper end surface of the detection table, the detection table is of a cavity structure with a rectangular axial section, guide ports are arranged at the positions of the side surfaces of the detection table corresponding to the bracket, operation ports are arranged at the upper end surface of the position corresponding to the bracket, the tray is of a disc structure coaxially distributed with the detection table, is embedded in the cavity of the detection table and is connected with the bottom of the cavity of the detection table through a rotary driving mechanism, at least two culture dishes are uniformly distributed around the axis of the tray and are connected with the upper end surface of the tray, and when the culture dishes are positioned at the operation ports, the culture dishes are coaxially distributed with the operation ports and are positioned outside the cavity of the detection table through the operation ports and the guide ports, the driving circuit is embedded in the bearing frame and is respectively connected with the detection table, the peristaltic pump is electrically connected with the rotary driving mechanism; the detection table is of any one of a rectangular cavity structure and a circular cavity structure, a transparent cover plate is arranged on the upper end face of the operation port position detection table, the transparent cover plate is in sliding connection with the side wall of the operation port through a sliding chute, a temperature and humidity sensor, a far infrared irradiation device and an ultraviolet irradiation device are arranged in the detection table, one temperature and humidity sensor, one far infrared irradiation device and one ultraviolet irradiation device form an adjusting group, the number of the adjusting groups is not less than two, each adjusting group is symmetrically distributed on two sides of a connecting line of central points of the two operation ports and distributed around the axis of the detection table, the temperature and humidity sensor, the far infrared irradiation device and the ultraviolet irradiation device are all connected with the top of the detection table, the axes of the temperature and humidity sensor, the far infrared irradiation device and the ultraviolet irradiation device are intersected with and vertically distributed on the upper end face of the tray, and the temperature and humidity sensor, the far infrared irradiation device and the ultraviolet irradiation device are all electrically connected with a driving circuit; the transparent cover plate is of an L-shaped groove structure in cross section, when the transparent cover plate and the operation port are coaxially distributed, the transparent cover plate is respectively covered outside the operation port and the guide port and forms a closed cavity structure with the detection platform, a partition plate is arranged in the cavity of the detection platform, the partition plate is respectively connected with the upper end surface of the detection platform and vertically distributed and distributed along the direction of a connecting line of central points of the two operation ports, the interior of the detection platform is divided into two cultivation cavities through the partition plate, the bottom of the detection platform corresponding to each cultivation cavity is provided with an inspection port, the tray is of a frame structure with a cross section in a shape like the Chinese character 'U', the bottom of the tray is of a grating plate structure, the upper end surface of the tray is provided with a plurality of elastic supporting blocks which are uniformly distributed around the axis of the tray, the height of each elastic supporting block is not more than 60% of the depth of the tray, and the upper end surface of the tray is provided with a sucking disc, the tray comprises a cross arm, an upright post and a slide block, wherein two ends of the cross arm are in sliding connection and vertical distribution with side surfaces of the upright post through the slide block, the rear end surface of the slide block is in sliding connection with side surfaces of the upright post through a slide groove, the slide groove is embedded in the side surfaces of the upright post and is in parallel distribution with the axis of the upright post, the front end surface of the slide block is hinged with the end surface of the cross arm through a ratchet mechanism, an included angle of 0-90 degrees is formed between the lower end surface of the cross arm and the axis of the upright post, the mixer, the storage tank, the peristaltic pump and the microscope are all connected with the cross arm, the mixer is connected with the lower end surface of the cross arm, and the driving circuit is a circuit system based on any one of a DSP chip and an FPGA chip.

2. The detection method of the primula cloaca pollen viability detection system based on claim 1, characterized in that: the detection method of the primula sikkmensis hook pollen activity detection system comprises the following steps: