CN113207502B

CN113207502B - Wild early cherry tissue culture rapid propagation system and method thereof

Info

Publication number: CN113207502B
Application number: CN202110666259.XA
Authority: CN
Inventors: 李蒙; 段一凡; 伊贤贵; 陈飞; 王贤荣
Original assignee: Nanjing Forestry University
Current assignee: Nanjing Forestry University
Priority date: 2021-06-16
Filing date: 2021-06-16
Publication date: 2022-02-08
Anticipated expiration: 2041-06-16
Also published as: CN113207502A

Abstract

The invention relates to a wild early cherry tissue culture rapid propagation system and a method thereof, and the system comprises a bearing box, an irradiation light source, a mixing tank, a peristaltic pump, a liquid collecting tank, a bearing tray, a guide slide rail and a driving circuit, wherein the guide slide rail is connected with the side wall of the bearing box, the liquid collecting tank is in sliding connection with the side wall of the bearing box through the guide slide rail, the bearing tray is in sliding connection with the side wall of the bearing box through the guide slide rail, the mixing tank is communicated with the peristaltic pump through a flow guide pipe, the peristaltic pump is respectively communicated with the liquid collecting tanks through flow dividing pipes, and the driving circuit is embedded on the outer side surface of the bearing box. The cultivation method comprises three steps of system prefabrication, stock germination cultivation and stock germination root development. On one hand, the invention can realize synchronous sprouting, root raising and seedling hardening, thereby effectively improving the working efficiency of plant cultivation operation; on the other hand, the cultivation regulator and the environment can be flexibly adjusted according to different stages of plant cultivation, and the success rate of cultivation and propagation is greatly improved.

Description

Wild early cherry tissue culture rapid propagation system and method thereof

Technical Field

The invention relates to a wild early cherry tissue culture rapid propagation system and a method thereof, belonging to the technical field of plant breeding.

Background

In the tissue culture and rapid propagation of wild early cherry, cutting propagation is one of important propagation means, and in order to meet the requirement of cutting propagation, a large number of related devices and technologies are currently developed, such as the tissue culture and rapid propagation method of the mountain cherry with publication number of CN111802247A, publication number of 20201023 and patent application number of 202010712527.2; the publication number is CN112293255A, the publication number is 20210202, the patent application number is 202011224241.6, and the patent name is a tissue culture rapid propagation method of large cherry rootstock Gisela No. 6; publication number is CN206835849U, publication date is 20180105, patent application number is 201720781292.6, the patent name is a tissue culture case that plant tissue culture used, although can satisfy the needs of breeding to a certain extent, it is complicated to cultivate the work flow, and cultivate and lack effectual linking cooperation between equipment, often need use multiple auxiliary assembly, and need carry out operation such as position change many times to the stock of cutting the cuttage reproduction, thereby lead to cultivating the work efficiency of operation low, and frequently change equipment and stock transfer and also lead to cultivating the manufacturing cost and the intensity of labour height of work, stock breeds and cultivates survival rate low, seriously influenced wild early cherry tissue culture and breed efficiency and the success rate of operation, lead to wild early cherry to cultivate the work of breeding and be difficult to develop smoothly.

Disclosure of Invention

In order to solve the defects in the prior art, the invention provides a wild early cherry tissue culture rapid propagation system and a method thereof, which can simultaneously realize the configuration and supply operation of nutrient solution and the regulation of culture temperature, humidity and illumination environment, thereby achieving the purpose of flexibly regulating culture regulator and environment according to different stages of plant culture and greatly improving the success rate of culture and propagation.

A wild early cherry tissue culture rapid propagation system comprises a bearing box, an irradiation light source, a mixing tank, a peristaltic pump, liquid collecting tanks, a bearing tray, a guide slide rail, a temperature and humidity sensor, an illuminometer, a spectrometer, a solid culture substrate and a driving circuit, wherein the guide slide rail is a plurality of, is uniformly distributed around the axis of the bearing box, is connected with the side wall of the bearing box and is vertically distributed at the bottom of the bearing box, the liquid collecting tanks are a plurality of and are embedded in the bearing box, are in sliding connection with the side wall of the bearing box through the guide slide rail and are distributed in parallel with the bottom of the bearing box, and are uniformly distributed in the same plane distributed in parallel with the bottom of the bearing box, the bearing tray is embedded in the bearing box and is coaxially distributed with the bearing box, is in sliding connection with the side wall of the bearing box through the guide slide rail and is positioned above each liquid collecting tank, a plurality of positioning holes are uniformly distributed on the bearing tray, and are uniformly distributed along the axis direction of the liquid collecting tanks, and the locating hole axis is perpendicular and crossing with the catch bowl axis, at least one of compounding jar, with bearing box outer surface connection to communicate through honeycomb duct and peristaltic pump, the peristaltic pump in addition communicates with one of them end of each catch bowl respectively through the shunt tubes, solid culture medium is located the bearing box to be located and bear the weight of tray below, temperature and humidity sensor inlays in bearing box bottom to with the coaxial distribution of bearing box, illuminometer, spectrum appearance are all at least two, encircle bearing box axis equipartition and inlay in bearing box up end, at least one of irradiation light source is located bearing box top at least 5 centimetres department to be connected with the bearing box lateral surface through coupling mechanism, drive circuit inlays in bearing box lateral surface to respectively with irradiation light source, compounding jar, peristaltic pump, temperature and humidity sensor, illuminometer, spectrum appearance electrical connection.

Furthermore, the liquid collecting tank is of a U-shaped groove body structure in cross section, a hard porous adsorption layer with the thickness not less than 5 mm is arranged at the bottom of the groove body, and a sewage draining outlet is formed in the bottom of the side wall of the bearing box and is communicated with the mixing tank through a guide pipe.

Furthermore, the irradiation light source comprises a bearing shell, a semiconductor heat dissipation mechanism, a bearing panel, at least one far infrared irradiation light source, at least one ultraviolet irradiation light source and at least four white light irradiation light sources, wherein the lower end surface of the bearing shell is connected with the bearing panel to form a closed cavity structure with a rectangular cross section, the semiconductor heat dissipation mechanism is embedded in the bearing shell, a plurality of heat dissipation ports are arranged on the upper end surface of the bearing shell corresponding to the semiconductor heat dissipation mechanism, the far infrared irradiation light sources, the ultraviolet irradiation light sources and the white light irradiation light sources are all embedded in the bearing panel, the white light irradiation light sources and the bearing panel are coaxially distributed, the optical axis of the white light irradiation light sources is vertical to the surface of the bearing panel, the far infrared irradiation light sources and the ultraviolet irradiation light sources are uniformly distributed around the white light irradiation light sources and are distributed at intervals between the far infrared irradiation light sources and the ultraviolet irradiation light sources, the area of the white light irradiation light source is 50% -70% of the area of the bearing panel, the areas of the far infrared irradiation light source and the ultraviolet irradiation light source are 10% -25% of the area of the bearing panel, and the area of the bearing panel is 10% -50% of the area of the upper end face of the bearing box.

Further, the locating hole all set for a position anchor clamps, positioning anchor clamps include spring cushion, shell fragment, base, holding ring and guiding arm, the base is two at least, encircle locating hole axis equipartition and with locating hole lateral wall sliding connection, the terminal surface passes through the shell fragment before the base and is connected with spring cushion, the base up end passes through the guiding arm and is connected with a holding ring, the guiding arm is articulated through the elasticity hinge between base and holding ring, and the guiding arm axis with bear the tray up end and be 0-90 contained angles, spring cushion and holding ring are the circular arc structure with the coaxial distribution of locating hole, and coaxial distribution between the spring cushion of same pedestal connection and the holding ring.

Further, coupling mechanism include spout, slider, flexible snakelike pole, universal hinge, wherein the spout is connected with the bearing box lateral surface to it is closed loop configuration to encircle the bearing box axis, slider and spout sliding connection are passed through to flexible snakelike pole lower terminal surface, and articulated through universal hinge between flexible snakelike pole and slider, flexible snakelike pole up end is articulated through universal hinge and irradiation light source lateral surface.

Further, the solid culture medium is composed of the following substances in parts by mass: 10-15% of coconut coir, 3-7% of perlite, 1-5% of bagasse, 0-3% of biogas residue, 0-2.5% of vinasse and the balance of nutrient soil.

Furthermore, the driving circuit is based on any one of a DSP chip and an FPGA chip, and is additionally provided with an operation interface based on a display, an operation keyboard and a wiring port, and the operation interface is embedded on the outer side surface of the bearing box.

A cultivation method of a wild early cherry tissue culture rapid propagation system comprises the following steps:

s1, performing system prefabrication, namely firstly assembling a bearing box, an irradiation light source, a mixing tank, a peristaltic pump, a liquid collecting tank, a bearing tray, a guide slide rail, a temperature and humidity sensor, an illuminometer, a spectrometer and a driving circuit, then adjusting the positions of the liquid collecting tanks to enable the distance between the lower end face of the liquid collecting tank and the bottom of the bearing box to be not less than 1/2 of the depth of the bearing box, then filling a solid culture medium into the bearing box, enabling the upper end face of the solid culture medium to be located below the bottom of the liquid collecting tank, and simultaneously adding and mixing raw material medicaments into the mixing tank to prepare a nutrient solution for cultivation for later use;

s2, germinating and cultivating the rootstocks, after the step S1 is carried out, inserting the rootstocks to be cultivated into positioning holes of a bearing tray, tightly fixing and positioning the rootstocks through positioning clamps of the positioning holes, enabling the lower end faces of the rootstocks after positioning to be embedded into liquid collecting grooves and abutted against the bottom of the liquid collecting grooves, simultaneously keeping 1-5 bud embryo tissues on the rootstocks positioned on the upper end faces of the bearing tray, driving a peristaltic pump to operate, conveying nutrient solution prepared in the step S1 into the liquid collecting grooves, soaking the bottom of the rootstocks, enabling the nutrient solution to overflow from the liquid collecting grooves at a constant speed, collecting and caching the nutrient solution by solid culture matrixes, driving a radiation light source to operate, regulating the cultivation temperature of the rootstocks through the radiation light source on one hand, regulating the cultivation illumination environment of the rootstocks on the other hand, and simultaneously realizing the cultivation temperature through a temperature and humidity sensor, a illuminometer and a spectrometer, Monitoring humidity, illumination intensity and spectrum;

s3, when the stock sprouts to grow roots, after the stock sprouting rate of the stock to be cultivated in the same liquid collecting tank is larger than 80% and the sprouting degree of the sprouting stock is larger than 5 mm through the S2 step, the liquid collecting tank and the whole stock connected with the liquid collecting tank move downwards through a guide sliding rail, the liquid collecting tank and part of the stock are embedded into a solid culture matrix, the stock depth of the solid culture matrix embedded above the liquid collecting tank is not smaller than 1 cm, then the nutrient solution prepared in the S1 step is continuously supplied to the liquid collecting tank, meanwhile, the temperature, humidity and illumination of the stock cultivation are adjusted through a radiation light source, the stock cultivation is realized, and when the new root length of the stock is not smaller than 10 mm and the number of roots is not less than 5, the stock cultivation can be completed.

The system has simple structure, flexible and convenient use and good universality, can simultaneously meet the requirements of centralized cultivation operation of various plant structure types, and realizes synchronous sprouting, root-raising and seedling hardening, thereby effectively improving the working efficiency of the plant cultivation operation; on the other hand, the preparation and supply operation of nutrient solution and the regulation of the cultivation temperature, humidity and illumination environment can be simultaneously realized in the cultivation process, so that the aim of flexibly regulating the cultivation regulator and the environment according to different stages of plant cultivation is fulfilled, and the success rate of cultivation and propagation is greatly improved.

Drawings

The invention is described in detail below with reference to the drawings and the detailed description;

FIG. 1 is a schematic diagram of the system of the present invention.

The reference numbers in the figures: the device comprises a bearing box 1, an irradiation light source 2, a mixing tank 3, a peristaltic pump 4, a liquid collecting tank 5, a bearing tray 6, a guide sliding rail 7, a temperature and humidity sensor 8, an illuminometer 9, a spectrometer 10, a solid culture medium 11, a driving circuit 12, a positioning hole 13, a shunt tube 14, a connecting mechanism 15, a sewage outlet 101, a bearing shell 21, a semiconductor heat dissipation mechanism 22, a bearing panel 23, a far infrared irradiation light source 24, an ultraviolet irradiation light source 25, a white light irradiation light source 26, an elastic cushion block 131, an elastic sheet 132, a base 133, a positioning ring 134, a guide arm 135, a sliding groove 151, a sliding block 152, a flexible serpentine rod 153 and a universal hinge 154.

Detailed Description

In order to facilitate the implementation of the technical means, creation features, achievement of the purpose and the efficacy of the invention, the invention is further described below with reference to specific embodiments.

As shown in figure 1, a wild early cherry tissue culture rapid propagation system comprises a bearing box 1, an irradiation light source 2, a mixing tank 3, a peristaltic pump 4, liquid collecting grooves 5, a bearing tray 6, a guide slide rail 7, a temperature and humidity sensor 8, an illuminometer 9, a spectrometer 10, a solid culture substrate 11 and a drive circuit 12, wherein the bearing box 1 is of a cavity structure with a U-shaped cross section, a plurality of guide slide rails 7 are uniformly distributed around the axis of the bearing box 1, are connected with the side wall of the bearing box 1 and are vertically distributed with the bottom of the bearing box 1, a plurality of liquid collecting grooves 5 are embedded in the bearing box 1, are slidably connected with the side wall of the bearing box 1 through the guide slide rails 7 and are parallelly distributed with the bottom of the bearing box 1, the liquid collecting grooves 5 are uniformly distributed in the same plane which is parallelly distributed with the bottom of the bearing box 1, the bearing tray 6 is embedded in the bearing box 1 and is coaxially distributed with the bearing box 1, is slidably connected with the side wall of the bearing box 1 through the guide slide rails 7 and is positioned above the liquid collecting grooves 5, a plurality of positioning holes 13 are uniformly distributed on the bearing tray 6, the positioning holes 13 are uniformly distributed along the axial direction of the liquid collecting tank 5, the axial lines of the positioning holes 13 are vertical to and intersected with the axial line of the liquid collecting tank 5, at least one material mixing tank 3 is connected with the outer surface of the bearing box 1 and is communicated with the peristaltic pump 4 through a flow guide pipe, the peristaltic pump 4 is respectively communicated with one end of each liquid collecting tank 5 through a flow dividing pipe 14, the solid culture substrate 11 is positioned in the bearing box 1 and is positioned below the bearing tray 6, the temperature and humidity sensor 8 is embedded at the bottom of the bearing box 1 and is coaxially distributed with the bearing box 1, at least two illuminometers 9 and spectrometers 10 are uniformly distributed around the axial line of the bearing box 1 and are embedded on the upper end surface of the bearing box 1, at least one irradiation light source 2 is positioned at least 5 cm above the bearing box 1 and is connected with the outer side surface of the bearing box 1 through a connecting mechanism 15, the driving circuit 12 is embedded on the outer side surface of the bearing box 1, and are respectively and electrically connected with an irradiation light source 2, a mixing tank 3, a peristaltic pump 4, a temperature and humidity sensor 8, an illuminometer 9 and a spectrometer 10.

In this embodiment, liquid trap 5 for the cross-section be "U" font cell body structure, the stereoplasm porous adsorption layer that thickness is not less than 5 millimeters is established to its cell body bottom, bear 1 lateral wall bottoms of case and establish drain 101, drain 101 communicates with mixing jar 3 through the honeycomb duct in addition, and can establish the booster pump on the honeycomb duct between drain 101 and mixing jar 3 in addition to improve waste liquid recovery efficiency, thereby realize waste liquid recovery reuse's purpose.

In this embodiment, the irradiation light source 2 includes a bearing shell 21, a semiconductor heat dissipation mechanism 22, a bearing panel 23, a far infrared irradiation light source 24, an ultraviolet irradiation light source 25 and a white light irradiation light source 26, wherein the lower end surface of the bearing shell 21 is connected with the bearing panel 23 to form a closed cavity structure with a rectangular cross section, at least one semiconductor heat dissipation mechanism 22 is embedded in the bearing shell 21, a plurality of heat dissipation ports 27 are formed on the upper end surface of the bearing shell 21 corresponding to the semiconductor heat dissipation mechanism 22, the far infrared irradiation light source 24, the ultraviolet irradiation light source 25 and the white light irradiation light source 26 are all embedded in the bearing panel 23, wherein the white light irradiation light source 26 and the bearing panel 23 are coaxially distributed, the optical axis thereof is perpendicular to the surface of the bearing panel 23, at least four far infrared irradiation light sources 24 and ultraviolet irradiation light sources 25 are all distributed around the white light irradiation light source 26, and the far infrared

irradiation light sources

24, 25 are uniformly distributed around the white light irradiation light source 26, The ultraviolet radiation light sources 25 are distributed at intervals, wherein the area of the white light radiation light source 26 is 50% -70% of the area of the bearing panel 23, the areas of the far infrared radiation light source 24 and the ultraviolet radiation light source 25 are 10% -25% of the area of the bearing panel 23, and the area of the bearing panel 23 is 10% -50% of the area of the upper end face of the bearing box 1.

It should be noted that, the positioning holes 13 are all provided with positioning fixtures, each positioning fixture includes at least two elastic cushion blocks 131, elastic pieces 132, a base 133, a positioning ring 134 and two guide arms 135, the bases 133 are uniformly distributed around the axis of the positioning holes 13 and are slidably connected with the side walls of the positioning holes 13, the front end face of the base 133 is connected with the elastic cushion blocks 131 through the elastic pieces 132, the upper end face of the base 133 is connected with one positioning ring 134 through the guide arms 135, the guide arms 135 are hinged to the base 133 and the positioning ring 134 through elastic hinges, the axis of the guide arms 135 forms an included angle of 0-90 degrees with the upper end face of the carrying tray 6, the elastic cushion blocks 131 and the positioning rings 134 are both arc structures coaxially distributed with the positioning holes 13, and the elastic cushion blocks 131 and the positioning rings 134 connected with the same base 133 are coaxially distributed.

In addition, the connecting mechanism 15 includes a sliding groove 151, a sliding block 152, a flexible serpentine rod 153, and a universal hinge 154, wherein the sliding groove 151 is connected to the outer side of the carrying case 1 and is a closed ring structure around the axis of the carrying case 1, the lower end surface of the flexible serpentine rod 153 is slidably connected to the sliding groove 151 through the sliding block 152, the flexible serpentine rod 153 is hinged to the sliding block 152 through the universal hinge 154, and the upper end surface of the flexible serpentine rod 153 is hinged to the outer side of the irradiation light source 2 through the universal hinge 154.

Further optimally, the solid culture substrate 11 is composed of the following substances in parts by mass: 10-15% of coconut coir, 3-7% of perlite, 1-5% of bagasse, 0-3% of biogas residue, 0-2.5% of vinasse and the balance of nutrient soil.

Meanwhile, the driving circuit 12 is based on any one of a DSP chip and an FPGA chip, and the driving circuit 12 is additionally provided with an operation interface based on a display, an operation keyboard and a wiring port, and the operation interface is embedded on the outer side surface of the carrying case 1.

s1, performing system prefabrication, namely firstly assembling a bearing box 1, an irradiation light source 2, a mixing tank 3, a peristaltic pump 4, a liquid collecting tank 5, a bearing tray 6, a guide slide rail 7, a temperature and humidity sensor 8, an illuminometer 9, a spectrometer 10 and a driving circuit 12, then adjusting the positions of the liquid collecting tanks 5 to enable the space between the lower end face of the liquid collecting tank 5 and the bottom of the bearing box 1 to be not less than 1/2 of the depth of the bearing box 1, then filling a solid culture matrix 11 into the bearing box 1, enabling the upper end face of the solid culture matrix 11 to be located below the bottom of the liquid collecting tank 5, simultaneously adding raw material medicaments into the mixing tank 3 and mixing to prepare a nutrient solution for cultivation for later use;

s2, germinating and cultivating the rootstocks, after the step S1 is carried out, inserting the rootstocks to be cultivated into the positioning holes 13 of the bearing tray 6, tightly fixing and positioning the rootstocks through the positioning clamps of the positioning holes 13, enabling the lower end faces of the rootstocks after positioning to be embedded into the liquid collecting tank 5 and to be abutted against the bottom of the liquid collecting tank 5, simultaneously keeping 1-5 bud embryo tissues on the rootstocks positioned on the upper end face of the bearing tray 6, then driving the peristaltic pump 4 to operate, conveying the nutrient solution prepared in the step S1 into the liquid collecting tank 5, soaking the bottom of the rootstocks, simultaneously enabling the nutrient solution to overflow from the liquid collecting tank 5 at a constant speed, collecting and caching the nutrient solution by the solid culture matrix 11, and driving the irradiation light source 2 to operate, on one hand, regulating the cultivation temperature of the rootstocks through the irradiation light source 2, on the other hand, regulating the cultivation light environment of the rootstocks, and simultaneously regulating the temperature and humidity sensor 8, The illuminometer 9 and the spectrometer 10 realize monitoring of the cultivation temperature, humidity, illumination intensity and spectrum;

s3, when the stock sprouts to grow roots, after the stock sprouting rate of the stock to be cultivated in the same liquid collecting tank 5 is larger than 80% and the sprouting degree of the sprouting stock is larger than 5 mm through the S2 cultivation, the liquid collecting tank 5 and the whole stock connected with the liquid collecting tank 5 move downwards through the guide slide rail 7, the liquid collecting tank 5 and part of the stock are embedded into the solid culture matrix 11, the stock depth of the solid culture matrix 11 above the liquid collecting tank 5 is not smaller than 1 cm, then the nutrient solution prepared in the S1 step is continuously supplied into the liquid collecting tank 5, meanwhile, the stock cultivation temperature, humidity and illumination are adjusted through the irradiation light source 2, the stock cultivation is realized, and when the length of the root system of the newly born stock is not smaller than 10 mm and the number of the root system is not smaller than 5, the stock cultivation can be completed.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a quick reproductive system of wild early cherry tissue culture which characterized in that: comprises a bearing box (1), an irradiation light source (2), a mixing tank (3), a peristaltic pump (4), a liquid collecting tank (5), a bearing tray (6), a plurality of guide slide rails (7), a temperature and humidity sensor (8), an illuminometer (9), a spectrometer (10), a solid culture medium (11) and a drive circuit (12), wherein the guide slide rails (7) are uniformly distributed around the axis of the bearing box (1), are connected with the side wall of the bearing box (1) and are vertically distributed with the bottom of the bearing box (1), the liquid collecting tanks (5) are embedded in the bearing box (1), are in sliding connection with the side wall of the bearing box (1) through the guide slide rails (7) and are distributed with the bottom of the bearing box (1) in parallel, and are uniformly distributed in the same plane distributed with the bottom of the bearing box (1), the bearing tray (6) is embedded in the bearing box (1) and is coaxially distributed with the bearing box (1), through direction slide rail (7) and bearing box (1) lateral wall sliding connection and be located each collecting tank (5) top, bear a plurality of locating holes (13) of equipartition on tray (6), locating hole (13) are along collecting tank (5) axis direction equipartition, and locating hole (13) axis and collecting tank (5) axis are perpendicular and crossing, mixing tank (3) at least one, with bear box (1) surface connection to communicate through honeycomb duct and peristaltic pump (4), peristaltic pump (4) communicate with one of them end of each collecting tank (5) respectively through shunt tubes (14) in addition, solid culture medium (11) are located and bear box (1) to be located and bear tray (6) below, temperature and humidity sensor (8) inlay in bearing box (1) bottom to with bear box (1) coaxial distribution, illuminometer (9), The spectrometer (10) is at least two, encircles and bears case (1) axis equipartition and inlays in bearing case (1) up end, irradiation light source (2) at least one is located bearing case (1) top at least 5 centimetres department to be connected with bearing case (1) lateral surface through coupling mechanism (15), drive circuit (12) inlay in bearing case (1) lateral surface, and respectively with irradiation light source (2), compounding jar (3), peristaltic pump (4), temperature and humidity sensor (8), illuminometer (9), spectrometer (10) electrical connection, collecting tank (5) for the cross section be "U" font cell body structure, the porous adsorbed layer of stereoplasm that thickness is not less than 5 millimeters is established to its cell body bottom, bear case (1) lateral wall bottom and establish drain (101), drain (101) communicate through honeycomb duct and compounding jar (3) in addition, the irradiation light source (2) comprises a bearing shell (21), a semiconductor heat dissipation mechanism (22), a bearing panel (23), a far infrared irradiation light source (24), an ultraviolet irradiation light source (25) and a white light irradiation light source (26), wherein the lower end face of the bearing shell (21) is connected with the bearing panel (23) and forms a closed cavity structure with a rectangular cross section, at least one semiconductor heat dissipation mechanism (22) is embedded in the bearing shell (21), a plurality of heat dissipation ports are formed in the upper end face of the bearing shell (21) corresponding to the semiconductor heat dissipation mechanism (22), the far infrared irradiation light source (24), the ultraviolet irradiation light source (25) and the white light irradiation light source (26) are all embedded in the bearing panel (23), the white light irradiation light source (26) and the bearing panel (23) are coaxially distributed, the optical axis of the white light irradiation light source is vertically distributed with the surface of the bearing panel (23), and the far infrared irradiation light source (24) and the white light irradiation light source (26) are arranged, The ultraviolet irradiation light sources (25) are at least four, are uniformly distributed around the white light irradiation light sources (26) and are distributed at intervals between the far infrared irradiation light sources (24) and the ultraviolet irradiation light sources (25), wherein the area of the white light irradiation light sources (26) is 50% -70% of the area of the bearing panel (23), the area of the far infrared irradiation light sources (24) and the area of the ultraviolet irradiation light sources (25) are 10% -25% of the area of the bearing panel (23), the area of the bearing panel (23) is 10% -50% of the area of the upper end face of the bearing box (1), positioning holes (13) are provided with positioning clamps, each positioning clamp comprises an elastic cushion block (131), an elastic sheet (132), a base (133), positioning rings (134) and guide arms (135), the bases (133) are at least two, are uniformly distributed around the axes of the positioning holes (13) and are in sliding connection with the side walls of the positioning holes (13), the utility model discloses a bearing tray, including base (133), locating hole (13), elastic sheet (132), locating ring (134), guide arm (135), locating ring (134), elastic sheet (131) and locating ring (134), base (133) preceding terminal surface is connected with elastic sheet (132), base (133) up end is connected with a locating ring (134) through guide arm (135), guide arm (135) and base (133) and locating ring (134) are articulated through elastic hinge, and guide arm (135) axis and bear tray (6) up end and be 0-90 contained angle, elastic sheet (131) and locating ring (134) are the circular arc structure with locating hole (13) coaxial distribution, and coaxial distribution between elastic sheet (131) and locating ring (134) that same base (133) is connected.

2. The rapid propagation system for tissue culture of wild early cherry according to claim 1, wherein: connecting mechanism (15) include spout (151), slider (152), flexible serpentine pole (153), universal hinge (154), wherein spout (151) are connected with bearing box (1) lateral surface to it is closed loop configuration to encircle bearing box (1) axis, the terminal surface passes through slider (152) and spout (151) sliding connection under flexible serpentine pole (153), and just flexible serpentine pole (153) and slider (152) are articulated through universal hinge (154) within a definite time, flexible serpentine pole (153) up end is articulated through universal hinge (154) and irradiation light source (2) lateral surface.

3. The rapid propagation system for tissue culture of wild early cherry according to claim 1, wherein: the solid culture medium (11) is composed of the following substances in parts by mass: 10-15% of coconut coir, 3-7% of perlite, 1-5% of bagasse, 0-3% of biogas residue, 0-2.5% of vinasse and the balance of nutrient soil.

4. The rapid propagation system for tissue culture of wild early cherry according to claim 1, wherein: the drive circuit (12) is based on any one circuit system of a DSP chip and an FPGA chip, and the drive circuit (12) is additionally provided with an operation interface based on a display, an operation keyboard and a wiring port, and the operation interface is embedded on the outer side surface of the bearing box (1).

5. The cultivation method of the wild early cherry tissue culture rapid propagation system according to claim 1, characterized in that the method comprises the following steps: s1, prefabricating a system, namely firstly assembling a bearing box (1), an irradiation light source (2), a mixing tank (3), a peristaltic pump (4), liquid collecting tanks (5), a bearing tray (6), a guide slide rail (7), a temperature and humidity sensor (8), a luminometer (9), a spectrometer (10) and a driving circuit (12), then adjusting the positions of the liquid collecting tanks (5) to enable the space between the lower end face of the liquid collecting tank (5) and the bottom of the bearing box (1) to be not less than 1/2 of the depth of the bearing box (1), then filling a solid culture medium (11) into the bearing box (1), enabling the upper end face of the solid culture medium (11) to be located below the bottom of the liquid collecting tank (5), simultaneously adding a raw material medicament into the mixing tank (3) and mixing to prepare a nutrient solution for cultivation for later use; s2, after the step S1 is completed, the rootstocks to be cultivated are inserted into the positioning holes (13) of the bearing tray (6) and are tightly fixed and positioned by the positioning clamps of the positioning holes (13), the lower end faces of the rootstocks after being positioned are all embedded into the liquid collecting tank (5) and abut against the bottom of the liquid collecting tank (5), 1-5 bud embryo tissues are reserved on the rootstocks positioned on the upper end face of the bearing tray (6), then a peristaltic pump (4) is driven to operate, nutrient solution prepared in the step S1 is conveyed into the liquid collecting tanks (5), the bottoms of the rootstocks are soaked, the nutrient solution overflows from the liquid collecting tank (5) at a constant speed, the nutrient solution is collected and cached by a solid culture matrix (11), an irradiation light source (2) is driven to operate, the cultivation temperature of the rootstocks is adjusted by the irradiation light source (2), and the cultivation illumination environment of the rootstocks is adjusted, meanwhile, the cultivation temperature, humidity, illumination intensity and spectrum are monitored through a temperature and humidity sensor (8), an illuminometer (9) and a spectrometer (10); s3, when the stock sprouts to grow roots, after the stock sprouting rate of the stock to be cultivated in the same liquid collecting tank (5) is larger than 80% through the cultivation in the S2 step, and the sprouting degree of the sprouting shoots after sprouting is larger than 5 mm, the liquid collecting tank (5) and the whole stock connected with the liquid collecting tank are moved downwards through the guide slide rail (7), the liquid collecting tank (5) and part of the stock are embedded into the solid cultivation matrix (11), the stock depth of the solid cultivation matrix (11) above the liquid collecting tank (5) is not smaller than 1 cm, then the nutrient solution prepared in the S1 step is continuously supplied into the liquid collecting tank (5), meanwhile, the stock cultivation temperature, humidity and illumination are adjusted through the irradiation light source (2), cultivation of the stock is achieved, the stock is cultivated, the length of a new root system of the stock is not smaller than 10 mm, and the number of the root systems is not smaller than 5, and the stock cultivation can be completed.