CN211881580U - Tissue culture case that plant tissue culture used - Google Patents

Abstract

The utility model relates to a tissue culture case that plant tissue culture used, comprising a box body and a box cover, case lid demountable installation is on the box, be provided with logical liquid pipe on the box, logical liquid pipe intercommunication has the device of supply water or nutrient solution, be provided with the breather pipe on the case lid, the breather pipe intercommunication has and is used for letting in CO₂The device of (2), be provided with a plurality of gas pockets on the case lid, the case lid is the bilayer, and the inside cavity intercommunication of bilayer case lid has the connecting pipe that is used for letting in cooling water. The utility model discloses simple structure, it is convenient to use, adopts the utility model discloses cultivate, can effectively optimize vegetation environment through manual control dynamic adjustment, improve the photosynthetic rate of plant, promoted the growth and development of plant to obtain high-quality kindAnd (5) seedling.

Description

Tissue culture case that plant tissue culture used

Technical Field

The utility model relates to a plant incubator technical field, concretely relates to tissue culture case that plant tissue culture used.

Background

At present, in general sugar culture micropropagation, the plantlets are heterotrophic or mixotrophic for a long time by using sugar (such as sucrose, white granulated sugar, fructose and the like) as a main carbon source, and the sugar is regarded as an essential substance in plant tissue culture and added into a culture medium.

In traditional tissue culture, the microenvironment for plant growth is rarely studied, and the focus of research is on the formulation of the culture medium, the amount of hormones and the addition of organic substances. Due to the presence of sugars in the culture medium, in order to prevent contamination, generally small culture vessels are used or only small culture vessels are used. Therefore, the photosynthetic rate, rooting rate and rooting quality of the plants are greatly limited.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a: to the not enough of existence among the above-mentioned prior art, the utility model provides a tissue culture case that plant tissue culture used.

The utility model adopts the technical scheme as follows:

the utility model provides a tissue culture case that plant tissue culture used, includes box and case lid, and case lid demountable installation is provided with liquid pipe on the box, and liquid pipe intercommunication has the device of supply water or nutrient solution, is provided with the breather pipe on the case lid, and the breather pipe intercommunication has and is used for letting in CO₂The device, the case lid is provided with a plurality of gas pockets, and the case lid is the bilayer, and the inside cavity intercommunication of bilayer case lid has the connecting pipe that is used for letting in the cooling water.

Furthermore, the device for supplying water or nutrient solution is a pump connected with a water source or nutrient solution source, the pump is electrically connected with a controller, the supply amount of the water or nutrient solution can be controlled through the controller, and manual control dynamic adjustment required by plant growth is realized.

Further, for introducing CO₂Is arranged with CO₂A source connected pump electrically connected with a controller, through which the introduction of CO can be controlled₂The amount of the fertilizer is realized to provide the optimal photosynthetic raw material for the growth of plants.

Further, a temperature sensor is installed on the side, close to the box body, of the inner layer of the box cover, and the temperature sensor is electrically connected with a controller.

Furthermore, the connecting pipe includes outlet pipe and oral siphon, and the outlet pipe is located the top of case lid, and the oral siphon is close to the bottom of case lid, and the oral siphon is connected with the pump, and the pump electricity is connected with the controller. When the temperature sensor detects that the temperature in the box body is higher than the set temperature, the pump is controlled through the controller, so that cooling water is introduced to cool the water, and a proper temperature is provided for plant growth.

Further, the box body is made of opaque plastic, and the box cover is made of transparent or semitransparent plastic.

Further, the air hole is provided with an air hole cover matched with the air hole for closing.

Further, a handle is arranged on the box cover.

To sum up, owing to adopted above-mentioned technical scheme, the beneficial effects of the utility model are that:

adopt the utility model discloses tissue culture case is cultivateed, can conveniently optimize vegetation environment through manual control dynamic adjustment, provides the best CO for seedling propagation growth₂The device comprises a controller, a liquid inlet pipe, a liquid outlet pipe, a liquid inlet pipe, a liquid outlet pipe, a liquid; the introduction of CO from the vent pipe can be controlled by a controller₂The amount of the compound fertilizer is used for providing optimal photosynthetic raw materials for plant growth; when the temperature sensor detects that the temperature in the box body is higher than the set temperature, the cooling water is introduced into the double-layer box cover from the connecting pipe through the controller to cool the box, the suitable temperature is provided for the growth of plants, the photosynthetic rate of the plants is improved, and the promotion is realizedThe growth and development of plants are saved, the common greenhouse domestication link is saved, the rooting seedlings can generate a large amount of adventitious roots as soon as possible, the mixotrophic type is changed into the autotrophic type, and then high-quality seedlings are obtained; through using the utility model discloses an incubator, with the subculture with two into one in the process of rooting culture, the culture cycle has shortened more than 40%, has reduced the influence of the microbial contamination in the plant micropropagation production process by a wide margin, has eliminated the disorder in the aspect of plantlet physiology and form for the seedling quality is showing and is improving, has improved the rooting rate and the quality of rooting of plant, especially to woody plant, has greatly improved the rooting rate and the quality of rooting of plant, test tube seedling transplantation survival rate is showing and is improving; therefore, adopt the utility model discloses carry out plant sugar-free group and organize rapid propagation, can effectively save the investment, reduction in production cost compares with traditional micropropagation technique, and the seedling production combined cost reduces 30% on average, group banks up the simplification of production technology with earth, and the flow shortens, and the integrated level of technique and equipment improves, has reduced the operation technique degree of difficulty and labor intensity, changes in popularization and application on the large-scale production, can realize plug seedling commercial process, also can realize extensive container automatic batch production.

Drawings

FIG. 1 is a block diagram of the present invention;

labeled as: 1-box body, 2-box cover, 3-liquid pipe, 4-air pipe, 5-handle, 6-air hole, 7-culture medium, 8-water outlet pipe and 9-water inlet pipe.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention, i.e., the described embodiments are only some, but not all embodiments of the invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiment of the present invention, all other embodiments obtained by the person skilled in the art without creative work belong to the protection scope of the present invention.

It is noted that relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The present invention will be described in detail with reference to fig. 1.

Examples

The embodiment of the utility model provides a tissue culture case that plant tissue culture used, including box 1 and case lid 2, case lid 2 demountable installation is on box 1, is provided with liquid pipe 3 on the box 1, and liquid pipe 3 intercommunication has the device of supply water or nutrient solution, is provided with breather pipe 4 on case lid 2, and breather pipe 4 intercommunication has and is used for letting in CO₂The device of (2), be provided with a plurality of gas pockets 6 on the case lid (2), case lid (2) are the bilayer, and the inside cavity intercommunication of bilayer case lid (2) has the connecting pipe that is used for letting in cooling water.

The utility model relates to a tissue culture rooting seedling indoor sugar-free domestication stage culture container which adopts the plant sugar-free tissue culture rapid propagation technology to culture, can effectively carry out natural ventilation and forced ventilation, and is introduced with CO through the ventilation pipe 4₂As plant bodies instead of sugarThe carbon source promotes the transition of the heterotrophic growth to the autotrophic growth of the plants, and the illumination, the temperature and the CO required by the growth of the plants can be regulated and controlled by automatic control equipment and the like connected with the carbon source₂And the environment factors further change the seedling from the mixotrophic type to the autotrophic type so as to produce high-quality seedlings. In addition, porous inorganic substances such as vermiculite, perlite, fiber and F lor ia l ite (mixture of vermiculite and fiber) are placed in the box body 1 to serve as a culture medium 7, and a proper amount of nutrient solution and rooting agent are added, so that the rooting rate and the rooting quality of the plantlets can be greatly improved.

The device for supplying water or nutrient solution is a pump connected with a water source or nutrient solution source, the pump is electrically connected with a controller, the supply amount of the water or nutrient solution can be controlled through the controller, and manual control dynamic adjustment required for plant growth is realized.

Wherein for introducing CO₂Is arranged with CO₂A source-connected pump electrically connected to the controller, by means of which CO can be achieved₂Forced introduction and controlled introduction of CO₂In an amount to achieve optimal carbon source conditions for plant growth.

Wherein, the side of the inner layer of the box cover 2 close to the box body 1 is provided with a temperature sensor which is electrically connected with a controller; the connecting pipe includes outlet pipe 8 and oral siphon 9, and outlet pipe 8 is located the top of case lid 2, and oral siphon 9 is close to the bottom of case lid 2, and cold water is by getting into down by last outflow, and oral siphon 9 is connected with the pump, and the pump electricity is connected with the controller, detects when 1 interior temperature of box is higher than the settlement temperature as temperature sensor, through controller control pump to let in the cooling water and carry out the water-cooling, provide suitable temperature for vegetation.

The controllers can be the same controller, and a PLC controller can be adopted. PLC controller with model number S7-300 manufactured by SI EMENS company; the temperature sensor may be a DS18B20 temperature sensor manufactured by Gemini electronics.

The box body 1 is made of light-tight plastic and simulates the growth environment of a plant root system; the box cover 2 is made of transparent or semitransparent plastic which can transmit light, so that photosynthesis of plants can be realized, and meanwhile, the incubator is light in weight and convenient to move.

Wherein, the stomata 6 is provided with a matched stomata cover for sealing, and can be used for artificially regulating and controlling the natural ventilation volume of plants.

Wherein, be provided with handle 5 on case lid 2, the portability of reinforcing incubator on the one hand, on the other hand also makes things convenient for opening of case lid 2 to be used for the planting and the transfer of plant.

The foregoing is a primary feature of the present solution and its advantages, and it should be understood by those skilled in the art that the present solution is not limited by the above embodiments, and the above embodiments and descriptions are only illustrative of the principles of the present solution, and that various changes and modifications may be made therein without departing from the spirit and scope of the present solution, which fall within the scope of the appended claims and their equivalents.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a releasable connection, or an integral connection; can be mechanically or electrically connected; either directly or indirectly through an intermediary profile, or both. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Claims (8)

1. The utility model provides a tissue culture case that plant tissue culture used which characterized in that: including box (1) and case lid (2), case lid (2) demountable installation is on box (1), be provided with on box (1) and lead to liquid pipe (3), it has the device of supply water or nutrient solution to lead to liquid pipe (3) intercommunication, be provided with breather pipe (4) on case lid (2), breather pipe (4) intercommunication has and is used for letting in CO₂The device comprises a box cover (2), wherein a plurality of air holes (6) are formed in the box cover (2), the box cover (2) is double-layer, and connecting pipes for introducing cooling water are communicated with the inside of the double-layer box cover (2) in a hollow mode。

2. The tissue culture chamber for plant tissue culture according to claim 1, characterized in that: the device for supplying water or nutrient solution is a pump connected with a water source or nutrient solution source, and the pump is electrically connected with a controller.

3. The tissue culture chamber for plant tissue culture according to claim 1, characterized in that: said for introducing CO₂Is arranged with CO₂A source-connected pump electrically connected to a controller.

4. The tissue culture chamber for plant tissue culture according to claim 1, characterized in that: the temperature sensor is installed to nearly box (1) side of case lid (2) inlayer, the temperature sensor electricity is connected with the controller.

5. The tissue culture chamber for plant tissue culture according to claim 4, characterized in that: the connecting pipe includes outlet pipe (8) and oral siphon (9), outlet pipe (8) are located the top of case lid (2), oral siphon (9) are close to the bottom of case lid (2), oral siphon (9) are connected with the pump, the pump electricity is connected with the controller.

6. The tissue culture chamber for plant tissue culture according to claim 1, characterized in that: the box body (1) is made of light-tight plastic, and the box cover (2) is made of light-permeable transparent or semitransparent plastic.

7. The tissue culture chamber for plant tissue culture according to claim 1, characterized in that: the air hole (6) is provided with an air hole cover matched with the air hole (6) for sealing.

8. The tissue culture chamber for plant tissue culture according to claim 1, characterized in that: the box cover (2) is provided with a handle (5).

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