CN105123488A - Plant tissue culture rooting and seedling exercising integrated cultivating device and cultivating method thereof - Google Patents
Plant tissue culture rooting and seedling exercising integrated cultivating device and cultivating method thereof Download PDFInfo
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- CN105123488A CN105123488A CN201510542049.4A CN201510542049A CN105123488A CN 105123488 A CN105123488 A CN 105123488A CN 201510542049 A CN201510542049 A CN 201510542049A CN 105123488 A CN105123488 A CN 105123488A
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- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000005286 illumination Methods 0.000 claims abstract description 24
- 238000009423 ventilation Methods 0.000 claims abstract description 11
- 239000012530 fluid Substances 0.000 claims abstract description 9
- 238000004891 communication Methods 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims description 133
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 60
- 230000008635 plant growth Effects 0.000 claims description 49
- 230000007613 environmental effect Effects 0.000 claims description 46
- 230000012010 growth Effects 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 26
- 238000012258 culturing Methods 0.000 claims description 20
- 230000010354 integration Effects 0.000 claims description 18
- 235000015097 nutrients Nutrition 0.000 claims description 17
- 238000012937 correction Methods 0.000 claims description 15
- 230000001105 regulatory effect Effects 0.000 claims description 14
- 230000000243 photosynthetic effect Effects 0.000 claims description 13
- 238000003860 storage Methods 0.000 claims description 13
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- 230000003044 adaptive effect Effects 0.000 claims description 8
- 230000033228 biological regulation Effects 0.000 claims description 8
- 238000011534 incubation Methods 0.000 claims description 8
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- 230000000694 effects Effects 0.000 claims description 6
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- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 4
- 239000005416 organic matter Substances 0.000 claims description 4
- 239000003415 peat Substances 0.000 claims description 4
- 239000010451 perlite Substances 0.000 claims description 4
- 235000019362 perlite Nutrition 0.000 claims description 4
- 230000035479 physiological effects, processes and functions Effects 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000009923 sugaring Methods 0.000 claims description 4
- 239000013589 supplement Substances 0.000 claims description 4
- 230000001360 synchronised effect Effects 0.000 claims description 4
- 239000011573 trace mineral Substances 0.000 claims description 4
- 235000013619 trace mineral Nutrition 0.000 claims description 4
- 239000010455 vermiculite Substances 0.000 claims description 4
- 235000019354 vermiculite Nutrition 0.000 claims description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims description 4
- 230000003698 anagen phase Effects 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 18
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 39
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
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- 229910052760 oxygen Inorganic materials 0.000 description 4
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- 238000011160 research Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
- A01G31/06—Hydroponic culture on racks or in stacked containers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a plant tissue culture rooting and seedling exercising integrated cultivating device and a cultivating method thereof. The cultivating device comprises a plant cultivation system, an illumination system, a ventilation temperature-control system, a fluid supplying system, a CO2 supplying system and an environment control system. The plant cultivation system comprises a three-dimensional multi-layer cultivation frame and cultivation containers placed on the cultivation frame, each layer of multiple layers of the cultivation frame is provided with one cultivation container, and lifting seedling disc supports are arranged at the bottoms of the containers and used for suspending cultivation plants. The illumination system comprises lamp boxes, LED lamp tubes and fans, and the LED lamp tubes are arranged inside the lamp boxes and connected with the fans to be installed above the cultivation containers in an integrated mode without communication with the cultivation containers. On the premise of guaranteeing that space and time are saved, the cost is low, and the utilization rate of resources is high, the utilization rate of the resources and labor productivity are increased, the cultivation period is shortened, and the production cost is lowered.
Description
Technical field
The invention belongs to technical field of plant culture, relate to a kind of culture apparatus, particularly relate to that a kind of plant tissue culture is taken root, the culture apparatus of hardening integration; Meanwhile, the invention still further relates to a kind of cultural method of above-mentioned culture apparatus.
Background technology
In Plant Tissue Breeding, the growth of plantlet in vitro generally will through four-stage: 1. set up in vitro cuttings, 2. Fast-propagation, 3. take root, 4. greenhouse hardening, after above four-stage, and could as production seedling.Wherein take root difficulty, transition shoot survival percent is low is ubiquitous problem in Plant Tissue Breeding always.The condition such as light, temperature, water, gas improving plant growth is regulated by artificial environment, promote the photosynthesis of plantlet, with autotrophy with improve the environmental condition that plant takes root, promote growing of root system, and by regulating the environmental conditions such as humidity, illumination, wind speed, by plantlet in vitro take root and two stages of hardening unite two into one, and plantlet in vitro fast growth, the survival rate of cultivating are high, can directly be transplanted to greenhouse or land for growing field crops, cultivation cycle shortens 50%, improve 50% labor productivity, reduce production cost.Find in the retrieval of prior art document Investigation, China Patent No. is: 200610119240.9 disclose a kind of Aeroponic type plant sugar-free tissue culturing device, comprising: culture vessel, culturing rack, illumination system, environmental detection set, plant growth environment maintenance system.Plant growth environment maintenance system comprises CO2 source of the gas, magnetic valve, nutrient solution atomizing device, cooling device, air pump, spray pipe.CO
2source of the gas is connected with magnetic valve by pipeline, magnetic valve is connected with nutrient solution atomizing device by pipeline, nutrient solution atomizing device is connected with cooling device by pipeline, and cooling device is connected with air pump by pipeline, and air pump to be connected with culture vessel by pipeline and extends to culture vessel inside.
Name is called that the technology of Aeroponic type plant sugar-free tissue culturing device is difficult to implement in actual applications, mainly contains the reason of the following aspects: 1. CO2 can water-soluble generation carbonic acid, affects the supply of CO2 and the pH value of nutrient solution; 2. air pump can not be used for the mist that draws water, and air pump is easily damaged and nutrient solution atomizing can be caused to pollute; 3. the water smoke of prior art will could arrive culture vessel through piping, and in the way through piping, mist has become water droplet and hung on duct wall, and the amount that can enter culture vessel is little.Device cultivate to as if test-tube plantlet plantlet in vitro in other words, this is a microminiaturized seedling, very delicate, very easily dead, very high to the requirement of environmental condition, the moisture absorbed less than abundance is very easily dead, therefore, there is great limitation in the cultivation of prior art to plant tissue culture seedling.
Meanwhile, the spectral range that prior art adopts is unreasonable to the cultivation of plant, and spectral range is too narrow, only uses the blue light of 660nm ruddiness and 450nm, these two light waves are only the absorption peaks of plant chlorophyll, but not the absorption spectrum of photosynthesis of plant.Total institute is known, and it is 400-700nm that plant carries out photosynthetic effective energy light wave scope, and the blue light of 400-500nm and the red light of 600-700nm are contributed maximum to photosynthesis.In photosynthesis, ruddiness is conducive to sugar and carbohydrate synthesis, and the stipes accelerating to improve plant is grown.Blue light is conducive to the synthesis of protein, is formed with considerable influence to the growth of plant and young shoot, can suppress the elongation of plant and make plant form short strong shape, also can arrange Cell Differentiation, being beneficial to anthocyanidin, vitamin synthesis.Create the optimal conditions of plant growth, light wave type must be enriched.And the ratio of the red blue light of prior art is: 2:1, the ruddiness in this ratio can not meet plantlet growth demand, and culture efficiency is lower.
In addition, the sensor of prior art CO2 concentration is mounted in culture vessel, the humidity in culture vessel because plant cultivate needs, relative moisture generally all more than 90%, CO
2the best effort scope of concentration sensor is 0-80%, and relative moisture, more than 90%, will be drifted about, thus causes the mistake of information.The present invention is air outlet CO2 sensor being arranged on gas circulation, thus avoids the impact of high humidity, and it is more accurate to control.
In view of this, nowadays in the urgent need to designing a kind of new plant cultivation device, to overcome the above-mentioned defect of existing culture apparatus.
Summary of the invention
Technical problem to be solved by this invention is: provide that a kind of plant tissue culture is taken root, the culture apparatus of hardening integration, can improve the survival rate of transition seedling, significantly shorten cultivation cycle, reduces production cost.
In addition, the present invention also provides the cultural method of above-mentioned culture apparatus, can improve the survival rate of transition seedling, significantly shorten cultivation cycle, reduces production cost.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
Plant tissue culture is taken root, a culture apparatus for hardening integration, and described culture apparatus comprises: plant cultivation system, illumination system, ventilation temperature-controlling system, liquid-supplying system, CO2 feed system, environmental control system;
Described plant cultivation system comprises: stereoscopic multi-layer culturing rack, culture vessel, and culture vessel is placed on culturing rack, and in multiple layers of culturing rack, one deck arranges a culture vessel, and container bottom places liftable seedling disc carrier, for unsettled cultivation plant; Between each layer culture vessel, gas circulates mutually, but overall large culture space is airtight;
Described illumination system comprises lamp box, LED lamp tube, fan, and LED is placed in lamp box and is connected with fan, is arranged on above culture vessel, has a style of one's own, do not communicate with culture vessel;
Described ventilation temperature-controlling system comprises: compressor or temperature control equipment, adjustable speed fan, airduct; Compressor is arranged on the bottom of culture apparatus, and adjustable speed fan is arranged on the top of device, and adjustable speed fan is connected by airduct and each culture vessel; Arrange airduct at the rear side of each culture vessel, made it that circulated air is sent into each culture vessel uniformly, and then get back to adjustable speed fan return air inlet from the front portion of culture vessel;
Described CO2 feed system comprises: CO2 source of the gas, magnetic valve; CO2 source of the gas is connected with magnetic valve by pipeline, and is connected with the return air inlet of ventilating system adjustable speed fan; The CO2 of high concentration is fully mixed with air in advance in airduct;
Described liquid-supplying system comprises: liquid reservoir tank, water pump, feed liquid pipeline, water regulating valve; Water pump is placed in liquid reservoir tank, and liquid reservoir tank and feed liquid pipeline are connected, and water regulating valve is arranged on the water outlet of each container; Nutrient solution or water enter in the culture vessel of the most last layer of device by the power of pump and pipeline, then the effect of gravity is relied on to flow in the culture vessel of lower one deck, circulation like this, until arrive fluid storage tank after one deck of the most end, after filtering, sends into most last layer culture vessel again; Water inlet and the delivery port of each container are diagonal, the level control valve of the water outlet dress of each container, regulate arbitrarily the water-storage capacity in culture vessel as required;
Described environmental control system comprises: Temperature Humidity Sensor, CO2 concentration sensor, display screen, control cabinet, the air outlet that Temperature Humidity Sensor is installed on culture vessel inside, CO2 concentration sensor is arranged on blower fan; Described control cabinet, display screen, be arranged on the top of device, be connected with communication line between each parts of control system;
Described environmental control system comprises:
Plant variety identification module, in order to obtain the photo of plant, photo that is plant is overall or local is compared with the corresponding photo in floristics database, chooses the kind of plant variety corresponding to the highest photo of similarity as this plant;
Plant growth situation identification module, in order to the height by range sensor identification plant, judges the growth situation of plant simultaneously according to the growth time of plant; If the plant height data obtained are lower than the plant height of corresponding growth time, and the difference of height is greater than setting value, then think that this plant growth situation is not good enough;
Environmental parameter adaptive adjustment module, in order to according to plant variety, growth time section, plant growth situation, in conjunction with plant growth environment parameter control table, regulates corresponding environmental parameter automatically; Store every environmental parameter of each plant variety, each time period in plant growth environment parameter control table, and if record plant growth situation not good enough time, corresponding environmental parameter correction data; These correction data are some groups, for systemic circulation; Find that plant growth situation is improved after using one group of correction data, then improve the priority revising data, commending system preferentially uses.
Plant tissue culture is taken root, a culture apparatus for hardening integration, and described culture apparatus comprises plant cultivation system;
Described plant cultivation system comprises: stereoscopic multi-layer culturing rack, culture vessel, and culture vessel is placed on culturing rack, and in multiple layers of culturing rack, one deck arranges a culture vessel, and container bottom places liftable seedling disc carrier, for unsettled cultivation plant.
As a preferred embodiment of the present invention, between each layer culture vessel, gas circulates mutually, but overall large culture space is airtight.
As a preferred embodiment of the present invention, described culture apparatus also comprises illumination system; Described illumination system comprises lamp box, LED lamp tube, fan, and LED is placed in lamp box and is connected with fan, is arranged on above culture vessel, has a style of one's own, do not communicate with culture vessel.
As a preferred embodiment of the present invention, described culture apparatus also comprises ventilation temperature-controlling system, and ventilation temperature-controlling system comprises: compressor or temperature control equipment, adjustable speed fan, airduct;
Compressor is arranged on the bottom of culture apparatus, and adjustable speed fan is arranged on the top of device, and adjustable speed fan is connected by airduct and each culture vessel; Arrange airduct at the rear side of each culture vessel, made it that circulated air is sent into each culture vessel uniformly, and then get back to adjustable speed fan return air inlet from the front portion of culture vessel.
As a preferred embodiment of the present invention, described culture apparatus also comprises CO2 feed system, and CO2 feed system comprises: CO2 source of the gas, magnetic valve; CO2 source of the gas is connected with magnetic valve by pipeline, and is connected with the return air inlet of ventilating system adjustable speed fan; The CO2 of high concentration is fully mixed with air in advance in airduct.
As a preferred embodiment of the present invention, described culture apparatus also comprises liquid-supplying system, and liquid-supplying system comprises: liquid reservoir tank, water pump, feed liquid pipeline, water regulating valve;
Water pump is placed in liquid reservoir tank, and liquid reservoir tank and feed liquid pipeline are connected, and water regulating valve is arranged on the water outlet of each container;
Nutrient solution or water enter in the culture vessel of the most last layer of device by the power of pump and pipeline, then the effect of gravity is relied on to flow in the culture vessel of lower one deck, circulation like this, until arrive fluid storage tank after one deck of the most end, after filtering, sends into most last layer culture vessel again; Water inlet and the delivery port of each container are diagonal, the level control valve of the water outlet dress of each container, regulate arbitrarily the water-storage capacity in culture vessel as required.
As a preferred embodiment of the present invention, described culture apparatus also comprises environmental control system, and environmental control system comprises: Temperature Humidity Sensor, CO2 concentration sensor, display screen, control cabinet;
The air outlet that Temperature Humidity Sensor is installed on culture vessel inside, CO2 concentration sensor is arranged on blower fan; Described control cabinet, display screen, be arranged on the top of device, be connected with communication line between each parts of control system.
As a preferred embodiment of the present invention, described environmental control system comprises:
Plant variety identification module, in order to obtain the photo of plant, photo that is plant is overall or local is compared with the corresponding photo in floristics database, chooses the kind of plant variety corresponding to the highest photo of similarity as this plant;
Plant growth situation identification module, in order to the height by range sensor identification plant, judges the growth situation of plant simultaneously according to the growth time of plant; If the plant height data obtained are lower than the plant height of corresponding growth time, and the difference of height is greater than setting value, then think that this plant growth situation is not good enough;
Environmental parameter adaptive adjustment module, in order to according to plant variety, growth time section, plant growth situation, in conjunction with plant growth environment parameter control table, regulates corresponding environmental parameter automatically; Store every environmental parameter of each plant variety, each time period in plant growth environment parameter control table, and if record plant growth situation not good enough time, corresponding environmental parameter correction data; These correction data are some groups, for systemic circulation; Find that plant growth situation is improved after using one group of correction data, then improve the priority revising data, commending system preferentially uses.
Above-mentioned plant tissue culture is taken root, a cultural method for the culture apparatus of hardening integration, cultivates above current by plant is unsettled, and a best rhizospheric environment is created in the growth for root system of plant; Adopt sugar-free culture-medium and porous material as culture matrix in addition, according to physiology and the photosynthetic demand of plant Different growth phases, dynamic Regulate Environment controling parameters, improves constantly plant to the adaptive capacity of environment and photosynthetic capacity, reaches best culture effect.
As a preferred embodiment of the present invention, described cultural method specifically comprises the steps:
With the material of porous as culture matrix, culture matrix comprise in vermiculite, perlite, peat soil one or more; First culture matrix is carried out autoclaving, after add MS nutrient solution and stir, load in clean cave dish, the stem section preparing to take root or plantlet be transplanted in the dish of cave, the unsettled seedling disc carrier be placed in culture vessel is cultivated; Not sugaring and organic matter in MS nutrient solution, only add macroelement, molysite and trace element;
During beginning, regulate the cycle-index of water that humidity is remained on more than 95-100% by liquid-supplying system, after keeping a week, along with the prolongation of incubation time, be down to 85%, 75% and 65% respectively, until close to the humidity of extraneous planting environment; Meanwhile, the control of intensity of illumination grows from weak to strong, and within 0-1 days, does not turn on light, later along with the prolongation of incubation time increases to 20 μm of olm gradually
-2s
-1, 40 μm of olm
-2s
-1, 60 μm of olm
-2s
-1, 80 μm of olm
-2s
-1, 100 μm of olm
-2s
-1above; The speed of air-flow, from 10cm/s, 20cm/s, 40cm/s, 60cm/s, increases to 80cm/s; In a word, the regulation and control of all environmental conditions are all round Promoting plant growth, improve photosynthetic rate, and make plantlet adapt to planting environment conversion regulation and control from the environment in culture vessel to container gradually;
CO2 controlled concentration is set as: the time of C3 plant 1000-1500ppm, C4 plant 2000-2500ppm, CO2 supply is synchronous with light application time;
By above cultivation seedbed, mounted LED lamp case is as plant illumination light source, LED light spectral limit is the ruddiness of 400-700nm white light and 660nm, and its ratio is: 9:1; Because contained abundant blue light in white light, more suitably supplement some ruddiness, the effective photosynthetic rate promoting plantlet, makes plantlet grow better.
Beneficial effect of the present invention is: the plant tissue culture that the present invention proposes is taken root, the culture apparatus of hardening integration and cultural method thereof, space is saved in guarantee, save time, low cost, under the prerequisite of resources effective utilization rate, in conjunction with Plant Nutrition, plant growth surroundings regulate and control, aerodynamics, photoelectricity, the general principle of Equipment Landscape Gardening, make to which overcome plant tissue culture seedling rooting difficulty, transition shoot survival percent is low, cultivation cycle is long, the problems such as production cost is high, improve resource utilization and labor productivity to greatest extent, shorten cultivation cycle, reduce production cost.
The present invention supplies CO2, gas circulation, temperature control combine, make CO2 distribute and air-flow motion evenly, liquid-circulating and humid control are combined more be conducive to the production of plant elsewhere.
This invention simplifies the device of prior art, add liftable seedling disc carrier in container, make cultivation can take into account multiple training method, water planting, substrate culture, unsettled training simultaneously, and operate steadily, controllability is strong, and operating cost is low.
The present invention passes through the research of polynary environmental factor with the phase structure relation of growth and development of plants, the technology of environment conditioning technology and Plant Tissue Breeding is fully merged, the structure of device and Functional Design is made more to meet the demand of production and scientific research, the microenvironment conditions such as gas concentration, temperature, humidity and nutrient solution concentration needed for can allocating according to the kind of plant and growing state.Successfully will to take root, hardening two stages synthesis two will be one, and significantly shorten cultivation cycle, reduce production cost, and seedling quality significantly improve.
The present invention is realized by following cultural method, with the material of porous as culture matrix, such as: vermiculite, perlite, peat soil etc., first matrix is carried out autoclaving, after add MS nutrient solution (not sugaring and organic matter only add macroelement, molysite and trace element) and stir, load in clean cave dish, the stem section preparing to take root or plantlet are transplanted in the dish of cave, the unsettled seedling disc carrier be placed in culture vessel is cultivated.During beginning, regulate the cycle-index of water that humidity is remained on more than 95-100% by liquid-supplying system, after keeping a week, along with the prolongation of incubation time, be down to 85%, 75% and 65% respectively, until close to the humidity of extraneous planting environment.Meanwhile, the control of intensity of illumination grows from weak to strong, and within 0-1 days, does not turn on light, later along with the prolongation of incubation time increases to 20 μm of olm gradually
-2s
-1, 40 μm of olm
-2s
-1, 60 μm of olm
-2s
-1, 80 μm of olm
-2s
-1, 100 μm of olm
-2s
-1above.The speed of air-flow, from 10cm/s, 20cm/s, 40cm/s, 60cm/s, increases to 80cm/s.In a word, the regulation and control of all environmental conditions are all round Promoting plant growth, improve photosynthetic rate, and make plantlet adapt to planting environment conversion regulation and control from the environment in culture vessel to container gradually.Cultivate with this technical method, plant strain growth is healthy and strong, well developed root system, and due to need not sugar and agar, can transplant by carrying substrates, do not hinder root during transplanting, survival rate be high, fast growth, and management is simple.
The unsettled advantage of cultivating above current of plant is except cooling regulates humidity in addition, also again in the flowing of water and the increase of dissolved oxygen, promote the quick growth of root system of plant, and circulate number of times, the air humidity in control culture vessel easily and effectively by water.The present invention is that a best rhizospheric environment is created in the growth of root system of plant, the water of flowing, the increase of dissolved oxygen, the medium of sugar-free impel plant self to carry out photosynthesis, these factors can promote root system of plant to be formed and growth, significantly improve the rooting rate of plantlet.
CO2 controlled concentration of the present invention is: the time of C3 plant 1000-1500ppm, C4 plant 2000-2500ppm, CO2 supply is synchronous with light application time.
The present invention is by above cultivation seedbed, mounted LED lamp case is as plant illumination light source, and LED light spectral limit is the ruddiness of 400-700nm white light and 660nm, and its ratio is: 9:1.Because contained abundant blue light in white light, more suitably supplement some ruddiness, effectively can promote the photosynthetic rate of plantlet, plantlet is grown better
In addition, it is only uniform that LED case of the present invention sends, and is uniformity to the illumination on culture bed surface, and plant strain growth can be made so even, improves the production capacity of unit culture area and the quality of seedling.
The invention solves the growth and development of plants existed in traditional tissue cultures bad; physiology and appearance is disorderly, deformity, grows and delays or the problem such as death; compared with traditional method for plant tissue culture; plant growth of the present invention is healthy and strong, and seedling quality significantly improves, rooting rate reach 95% and on; cultivation cycle shortens 30-50%; improve 50% labor productivity, production cost reduces, and is conducive to the scale of plant fast propagation, factorial praluction.In addition, the present invention revolutionizes traditional group training technology and production pattern, and the dream of the fast numerous mechanization of plant tissue culture and Automated condtrol is achieved.
Accompanying drawing explanation
Fig. 1 is the composition schematic diagram of culture apparatus of the present invention.
Embodiment
The preferred embodiments of the present invention are described in detail below in conjunction with accompanying drawing.
Embodiment one
Present invention is disclosed that a kind of plant tissue culture is taken root, the culture apparatus of hardening integration, described culture apparatus comprises: plant cultivation system, illumination system, ventilation temperature-controlling system, liquid-supplying system, CO2 feed system, environmental control system.
Described plant cultivation system comprises: stereoscopic multi-layer culturing rack, culture vessel, and culture vessel is placed on culturing rack, and in multiple layers of culturing rack, one deck arranges a culture vessel, and container bottom places liftable seedling disc carrier, for unsettled cultivation plant; Between each layer culture vessel, gas circulates mutually, but overall large culture space is airtight.
Described illumination system comprises lamp box, LED lamp tube, fan, and LED is placed in lamp box and is connected with fan, is arranged on above culture vessel, has a style of one's own, do not communicate with culture vessel.
Described ventilation temperature-controlling system comprises: compressor or temperature control equipment, adjustable speed fan, airduct; Compressor is arranged on the bottom of culture apparatus, and adjustable speed fan is arranged on the top of device, and adjustable speed fan is connected by airduct and each culture vessel; Arrange airduct at the rear side of each culture vessel, made it that circulated air is sent into each culture vessel uniformly, and then get back to adjustable speed fan return air inlet from the front portion of culture vessel.
Described CO2 feed system comprises: CO2 source of the gas, magnetic valve; CO2 source of the gas is connected with magnetic valve by pipeline, and is connected with the return air inlet of ventilating system adjustable speed fan; The CO2 of high concentration is fully mixed with air in advance in airduct.
Described liquid-supplying system comprises: liquid reservoir tank, water pump, feed liquid pipeline, water regulating valve; Water pump is placed in liquid reservoir tank, and liquid reservoir tank and feed liquid pipeline are connected, and water regulating valve is arranged on the water outlet of each container; Nutrient solution or water enter in the culture vessel of the most last layer of device by the power of pump and pipeline, then the effect of gravity is relied on to flow in the culture vessel of lower one deck, circulation like this, until arrive fluid storage tank after one deck of the most end, after filtering, sends into most last layer culture vessel again; Water inlet and the delivery port of each container are diagonal, the level control valve of the water outlet dress of each container, regulate arbitrarily the water-storage capacity in culture vessel as required.
Described environmental control system comprises: Temperature Humidity Sensor, CO2 concentration sensor, display screen, control cabinet, the air outlet that Temperature Humidity Sensor is installed on culture vessel inside, CO2 concentration sensor is arranged on blower fan; Described control cabinet, display screen, be arranged on the top of device, be connected with communication line between each parts of control system.
Described environmental control system comprises: plant variety identification module, plant growth situation identification module, environmental parameter adaptive adjustment module.
Plant variety identification module, in order to obtain the photo of plant, photo that is plant is overall or local is compared with the corresponding photo in floristics database, chooses the kind of plant variety corresponding to the highest photo of similarity as this plant.
Plant growth situation identification module, in order to the height by range sensor identification plant, judges the growth situation of plant simultaneously according to the growth time of plant; If the plant height data obtained are lower than the plant height of corresponding growth time, and the difference of height is greater than setting value, then think that this plant growth situation is not good enough.
Environmental parameter adaptive adjustment module, in order to according to plant variety, growth time section, plant growth situation, in conjunction with plant growth environment parameter control table, regulates corresponding environmental parameter automatically; Store every environmental parameter of each plant variety, each time period in plant growth environment parameter control table, and if record plant growth situation not good enough time, corresponding environmental parameter correction data; These correction data are some groups, for systemic circulation; Find that plant growth situation is improved after using one group of correction data, then improve the priority revising data, commending system preferentially uses.
Particularly, refer to Fig. 1, culture apparatus of the present invention comprises: stereoscopic multi-layer culturing rack 1, culture vessel 2, seedling disc carrier 3, LED case 4, fan 5, fluid storage tank 6, water pump 7, feed pipe 8, water regulating valve 9, compressor 10, adjustable speed fan 11, airduct, CO2 source of the gas 13, magnetic valve, Temperature Humidity Sensor 15, CO2 concentration sensor 16, display screen 17, control cabinet 18.
Its connected mode is: culture vessel, culture vessel are placed on culturing rack, one deck culture vessel, and container bottom places liftable seedling disc carrier, for unsettled cultivation plant.Between each layer culture vessel, gas can circulate mutually, but overall large culture space is airtight.LED is placed in lamp box and is connected with fan, is arranged on above culture vessel, has a style of one's own, do not communicate with culture vessel.Water pump is placed in fluid storage tank, and fluid storage tank and feed liquid pipeline are connected, and water regulating valve is arranged on the water outlet of each container.Compressor is arranged on the bottom of culture apparatus, and adjustable speed fan is arranged on the top of device, and blower fan is connected by airduct and each culture vessel.CO2 source of the gas is connected with magnetic valve by pipeline, and be connected to the return air inlet of adjustable speed fan, CO2 concentration sensor is arranged on air outlet.The control cabinet of environmental control system, display screen, be arranged on the top of device, the air inlet that Temperature Humidity Sensor is installed on culture vessel inside, CO2 concentration sensor is arranged on blower fan, is connected with communication line between each parts of control system.
The present invention is realized by following cultural method: use the material of porous as culture matrix, such as: vermiculite, perlite, peat soil etc., first matrix is carried out autoclaving, after add MS nutrient solution (not sugaring and organic matter, only add macroelement, molysite and trace element) stir, load in clean cave dish, the stem section preparing to take root or plantlet are transplanted in the dish of cave, the unsettled seedling disc carrier be placed in culture vessel is cultivated.During beginning, regulate the cycle-index of water that humidity is remained on more than 95-100% by liquid-supplying system, after keeping a week, along with the prolongation of incubation time, be down to 85%, 75% and 65% respectively, until close to the humidity of extraneous planting environment.Meanwhile, the control of intensity of illumination grows from weak to strong, and within 0-1 days, does not turn on light, later along with the prolongation of incubation time increases to 20 μm of olm gradually
-2s
-1, 40 μm of olm
-2s
-1, 60 μm of olm
-2s
-1, 80 μm of olm
-2s
-1, 100 μm of olm
-2s
-1above.The speed of air-flow, from 10cm/s, 20cm/s, 40cm/s, 60cm/s, increases to 80cm/s.In a word, the regulation and control of all environmental conditions are all round Promoting plant growth, improve photosynthetic rate, and make plantlet adapt to planting environment conversion regulation and control from the environment in culture vessel to container gradually.Cultivate with this technical method, plant strain growth is healthy and strong, well developed root system, and due to need not sugar and agar, can transplant by carrying substrates, do not hinder root during transplanting, survival rate be high, fast growth, and management is simple.
The unsettled advantage of cultivating above current of plant is except cooling regulates humidity in addition, also again in the flowing of water and the increase of dissolved oxygen, promote the quick growth of root system of plant, and circulate number of times, the air humidity in control culture vessel easily and effectively by water.The present invention is that a best rhizospheric environment is created in the growth of root system of plant, the water of flowing, the increase of dissolved oxygen, the medium of sugar-free impel plant self to carry out photosynthesis, these factors can promote root system of plant to be formed and growth, significantly improve the rooting rate of plantlet.
CO2 controlled concentration of the present invention is: the time of C3 plant 1000-1500ppm, C4 plant 2000-2500ppm, CO2 supply is synchronous with light application time.
The present invention is by above cultivation seedbed, mounted LED lamp case is as plant illumination light source, and LED light spectral limit is the ruddiness of 400-700nm white light and 660nm, and its ratio is: 9:1.Because contained abundant blue light in white light, more suitably supplement some ruddiness, effectively can promote the photosynthetic rate of plantlet, plantlet is grown better.
In addition, it is only uniform that LED case of the present invention sends, and is uniformity to the illumination on culture bed surface, and plant strain growth can be made so even, improves the production capacity of unit culture area and the quality of seedling.
The invention solves the growth and development of plants existed in traditional tissue cultures bad; physiology and appearance is disorderly, deformity, grows and delays or the problem such as death; compared with traditional method for plant tissue culture; plant growth of the present invention is healthy and strong, and seedling quality significantly improves, rooting rate reach 95% and on; cultivation cycle shortens 30-50%; improve 50% labor productivity, production cost reduces, and is conducive to the scale of plant fast propagation, factorial praluction.In addition, the present invention revolutionizes traditional group training technology and production pattern, and the dream of the fast numerous mechanization of plant tissue culture and Automated condtrol is achieved.
In sum, the plant tissue culture that the present invention proposes is taken root, the culture apparatus of hardening integration and cultural method thereof, save space in guarantee, save time, low cost, under the prerequisite of resources effective utilization rate, in conjunction with the general principle of Plant Nutrition, plant growth surroundings regulate and control, aerodynamics, photoelectricity, Equipment Landscape Gardening, make to which overcome plant tissue culture seedling rooting difficulty, transition shoot survival percent is low, the problems such as cultivation cycle is long, and production cost is high, improve resource utilization and labor productivity to greatest extent, shorten cultivation cycle, reduce production cost.
Here description of the invention and application is illustrative, not wants by scope restriction of the present invention in the above-described embodiments.Distortion and the change of embodiment disclosed are here possible, are known for the replacement of embodiment those those of ordinary skill in the art and the various parts of equivalence.Those skilled in the art are noted that when not departing from spirit of the present invention or substantive characteristics, the present invention can in other forms, structure, layout, ratio, and to realize with other assembly, material and parts.When not departing from the scope of the invention and spirit, can other distortion be carried out here to disclosed embodiment and change.
Claims (10)
1. plant tissue culture take root, the culture apparatus of hardening integration, it is characterized in that, described culture apparatus comprises: plant cultivation system, illumination system, ventilation temperature-controlling system, liquid-supplying system, CO2 feed system, environmental control system;
Described plant cultivation system comprises: stereoscopic multi-layer culturing rack, culture vessel, and culture vessel is placed on culturing rack, and in multiple layers of culturing rack, one deck arranges a culture vessel, and container bottom places liftable seedling disc carrier, for unsettled cultivation plant; Between each layer culture vessel, gas circulates mutually, but overall large culture space is airtight;
Described illumination system comprises lamp box, LED lamp tube, fan, and LED is placed in lamp box and is connected with fan, is arranged on above culture vessel, has a style of one's own, do not communicate with culture vessel;
Described ventilation temperature-controlling system comprises: compressor or temperature control equipment, adjustable speed fan, airduct; Compressor is arranged on the bottom of culture apparatus, and adjustable speed fan is arranged on the top of device, and adjustable speed fan is connected by airduct and each culture vessel; Arrange airduct at the rear side of each culture vessel, made it that circulated air is sent into each culture vessel uniformly, and then get back to adjustable speed fan return air inlet from the front portion of culture vessel;
Described CO2 feed system comprises: CO2 source of the gas, magnetic valve; CO2 source of the gas is connected with magnetic valve by pipeline, and is connected with the return air inlet of ventilating system adjustable speed fan; The CO2 of high concentration is fully mixed with air in advance in airduct;
Described liquid-supplying system comprises: liquid reservoir tank, water pump, feed liquid pipeline, water regulating valve; Water pump is placed in liquid reservoir tank, and liquid reservoir tank and feed liquid pipeline are connected, and water regulating valve is arranged on the water outlet of each container; Nutrient solution or water enter in the culture vessel of the most last layer of device by the power of pump and pipeline, then the effect of gravity is relied on to flow in the culture vessel of lower one deck, circulation like this, until arrive fluid storage tank after one deck of the most end, after filtering, sends into most last layer culture vessel again; Water inlet and the delivery port of each container are diagonal, the level control valve of the water outlet dress of each container, regulate arbitrarily the water-storage capacity in culture vessel as required;
Described environmental control system comprises: Temperature Humidity Sensor, CO2 concentration sensor, display screen, control cabinet, the air outlet that Temperature Humidity Sensor is installed on culture vessel inside, CO2 concentration sensor is arranged on blower fan; Described control cabinet, display screen, be arranged on the top of device, be connected with communication line between each parts of control system;
Described environmental control system comprises:
Plant variety identification module, in order to obtain the photo of plant, photo that is plant is overall or local is compared with the corresponding photo in floristics database, chooses the kind of plant variety corresponding to the highest photo of similarity as this plant;
Plant growth situation identification module, in order to the height by range sensor identification plant, judges the growth situation of plant simultaneously according to the growth time of plant; If the plant height data obtained are lower than the plant height of corresponding growth time, and the difference of height is greater than setting value, then think that this plant growth situation is not good enough;
Environmental parameter adaptive adjustment module, in order to according to plant variety, growth time section, plant growth situation, in conjunction with plant growth environment parameter control table, regulates corresponding environmental parameter automatically; Store every environmental parameter of each plant variety, each time period in plant growth environment parameter control table, and if record plant growth situation not good enough time, corresponding environmental parameter correction data; These correction data are some groups, for systemic circulation; Find that plant growth situation is improved after using one group of correction data, then improve the priority revising data, commending system preferentially uses.
2. plant tissue culture take root, the culture apparatus of hardening integration, it is characterized in that, described culture apparatus comprises plant cultivation system;
Described plant cultivation system comprises: stereoscopic multi-layer culturing rack, culture vessel, and culture vessel is placed on culturing rack, and in multiple layers of culturing rack, one deck arranges a culture vessel, and container bottom places liftable seedling disc carrier, for unsettled cultivation plant.
3. plant tissue culture according to claim 2 take root, the culture apparatus of hardening integration, it is characterized in that:
Between each layer culture vessel, gas circulates mutually, but overall large culture space is airtight.
4. plant tissue culture according to claim 2 take root, the culture apparatus of hardening integration, it is characterized in that:
Described culture apparatus also comprises illumination system; Described illumination system comprises lamp box, LED lamp tube, fan, and LED is placed in lamp box and is connected with fan, is arranged on above culture vessel, has a style of one's own, do not communicate with culture vessel.
5. plant tissue culture according to claim 2 take root, the culture apparatus of hardening integration, it is characterized in that:
Described culture apparatus also comprises ventilation temperature-controlling system, and ventilation temperature-controlling system comprises: compressor or temperature control equipment, adjustable speed fan, airduct;
Compressor is arranged on the bottom of culture apparatus, and adjustable speed fan is arranged on the top of device, and adjustable speed fan is connected by airduct and each culture vessel; Arrange airduct at the rear side of each culture vessel, made it that circulated air is sent into each culture vessel uniformly, and then get back to adjustable speed fan return air inlet from the front portion of culture vessel.
6. plant tissue culture according to claim 2 take root, the culture apparatus of hardening integration, it is characterized in that:
Described culture apparatus also comprises CO2 feed system, and CO2 feed system comprises: CO2 source of the gas, magnetic valve; CO2 source of the gas is connected with magnetic valve by pipeline, and is connected with the return air inlet of ventilating system adjustable speed fan; The CO2 of high concentration is fully mixed with air in advance in airduct.
7. plant tissue culture according to claim 2 take root, the culture apparatus of hardening integration, it is characterized in that:
Described culture apparatus also comprises liquid-supplying system, and liquid-supplying system comprises: liquid reservoir tank, water pump, feed liquid pipeline, water regulating valve;
Water pump is placed in liquid reservoir tank, and liquid reservoir tank and feed liquid pipeline are connected, and water regulating valve is arranged on the water outlet of each container;
Nutrient solution or water enter in the culture vessel of the most last layer of device by the power of pump and pipeline, then the effect of gravity is relied on to flow in the culture vessel of lower one deck, circulation like this, until arrive fluid storage tank after one deck of the most end, after filtering, sends into most last layer culture vessel again; Water inlet and the delivery port of each container are diagonal, the level control valve of the water outlet dress of each container, regulate arbitrarily the water-storage capacity in culture vessel as required.
8. plant tissue culture according to claim 2 take root, the culture apparatus of hardening integration, it is characterized in that:
Described culture apparatus also comprises environmental control system, and environmental control system comprises: Temperature Humidity Sensor, CO2 concentration sensor, display screen, control cabinet;
The air outlet that Temperature Humidity Sensor is installed on culture vessel inside, CO2 concentration sensor is arranged on blower fan; Described control cabinet, display screen, be arranged on the top of device, be connected with communication line between each parts of control system;
Described environmental control system comprises:
Plant variety identification module, in order to obtain the photo of plant, photo that is plant is overall or local is compared with the corresponding photo in floristics database, chooses the kind of plant variety corresponding to the highest photo of similarity as this plant;
Plant growth situation identification module, in order to the height by range sensor identification plant, judges the growth situation of plant simultaneously according to the growth time of plant; If the plant height data obtained are lower than the plant height of corresponding growth time, and the difference of height is greater than setting value, then think that this plant growth situation is not good enough;
Environmental parameter adaptive adjustment module, in order to according to plant variety, growth time section, plant growth situation, in conjunction with plant growth environment parameter control table, regulates corresponding environmental parameter automatically; Store every environmental parameter of each plant variety, each time period in plant growth environment parameter control table, and if record plant growth situation not good enough time, corresponding environmental parameter correction data; These correction data are some groups, for systemic circulation; Find that plant growth situation is improved after using one group of correction data, then improve the priority revising data, commending system preferentially uses.
9. the plant tissue culture that one of claim 1 to 9 is described is taken root, a cultural method for the culture apparatus of hardening integration, and it is characterized in that: cultivate above current by plant is unsettled, a best rhizospheric environment is created in the growth for root system of plant; Adopt sugar-free culture-medium and porous material as culture matrix in addition, according to physiology and the photosynthetic demand of plant Different growth phases, dynamic Regulate Environment controling parameters, improves constantly plant to the adaptive capacity of environment and photosynthetic capacity.
10. cultural method according to claim 9, is characterized in that:
Described cultural method specifically comprises the steps:
With the material of porous as culture matrix, culture matrix comprise in vermiculite, perlite, peat soil one or more; First culture matrix is carried out autoclaving, after add MS nutrient solution and stir, load in clean cave dish, the stem section preparing to take root or plantlet be transplanted in the dish of cave, the unsettled seedling disc carrier be placed in culture vessel is cultivated; Not sugaring and organic matter in MS nutrient solution, only add macroelement, molysite and trace element;
During beginning, regulate the cycle-index of water that humidity is remained on more than 95-100% by liquid-supplying system, after keeping a week, along with the prolongation of incubation time, be down to 85%, 75% and 65% respectively, until close to the humidity of extraneous planting environment; Meanwhile, the control of intensity of illumination grows from weak to strong, and within 0-1 days, does not turn on light, later along with the prolongation of incubation time increases to 20 μm of olm gradually
-2s
-1, 40 μm of olm
-2s
-1, 60 μm of olm
-2s
-1, 80 μm of olm
-2s
-1, 100 μm of olm
-2s
-1above; The speed of air-flow, from 10cm/s, 20cm/s, 40cm/s, 60cm/s, increases to 80cm/s; In a word, the regulation and control of all environmental conditions are all round Promoting plant growth, improve photosynthetic rate, and make plantlet adapt to planting environment conversion regulation and control from the environment in culture vessel to container gradually;
CO2 controlled concentration is set as: the time of C3 plant 1000-1500ppm, C4 plant 2000-2500ppm, CO2 supply is synchronous with light application time;
By above cultivation seedbed, mounted LED lamp case is as plant illumination light source, LED light spectral limit is the ruddiness of 400-700nm white light and 660nm, and its ratio is: 9:1; Because contained abundant blue light in white light, more suitably supplement some ruddiness, the effective photosynthetic rate promoting plantlet, makes plantlet grow better.
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