CN106172009A - Plant sugar-free culture systems and plant cultivation container - Google Patents
Plant sugar-free culture systems and plant cultivation container Download PDFInfo
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- CN106172009A CN106172009A CN201610669136.0A CN201610669136A CN106172009A CN 106172009 A CN106172009 A CN 106172009A CN 201610669136 A CN201610669136 A CN 201610669136A CN 106172009 A CN106172009 A CN 106172009A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
Present invention is disclosed a kind of plant sugar-free culture systems and plant cultivation container, including: airtight culturing room, culturing rack, culture vessel, air mixing machine structure, CO2Storage container, electromagnetic valve, steam line, CO2Densitometer, air-conditioning；Place at least one culturing rack in described airtight culturing room, culturing rack is placed at least one culture vessel；CO2Storage container is connected with electromagnetic valve by steam line, and electromagnetic valve is by steam line simultaneously and CO2Densitometer is connected with the air outlet of air-conditioning；Described airtight culturing room is internal by air-conditioning temperature control, and the interior circulation of line space of going forward side by side gas, to ensure CO2The efficient utilization of gas；Described culture vessel can carry out natural ventilation and forced ventilation, can the effectively CO in adding container2And adjusting air humidity.The invention can ensure that plant in incubation, required illumination, CO2, temperature, humidity sufficiently met, particularly can regulate air velocity according to plant growing situation and photosynthesis, supplement CO2Gas and the effective supply of nutritional solution.
Description
Technical field
The invention belongs to technical field of plant culture, relate to a kind of plant culturing system, particularly relate to a kind of plant sugar-free
Culture systems.
Background technology
The target of micropropagation is to obtain that substantial amounts of heredity is identical, physiology is consistent, growth promoter is planted normally at short notice
Strain.But, in traditional plant tissue culture micropropagation, the carbon source of plantlet growth depends on the sugar in culture medium,
But sugar promotes the pollution of microorganism；For avoiding polluting, it has to use airtight small container to cultivate, cause plant physiology
The outstanding problem such as disorder, seedling poor quality, transplanting survival rate are low.And, small container complex operation, human resources consumes greatly, machine
Tool degree is low, it is difficult to realize the factorial praluction that plant tissue culture is the most numerous.
It is with CO that plant sugar-free cultivates micropropagation2Sugar in substitutive medium, as carbon source, uses artificial environment to control hands
Section, provides suitable illumination, temperature, humidity, CO for the growth of plantlet in container2The condition such as concentration, nutrition, thus promote to plant
The growth promoter of thing and Fast-propagation.Overcoming pollution rate in traditional group training high, growth and development of plants is bad, physiology and appearance is disorderly
Disorderly, deformity, growth promoter delay or the problem such as death；It is remarkably improved seedling quality, shortens cultivation cycle, improve work and produce
Rate, reduces production cost.
But, although plant sugar-free is cultivated the research of micropropagation and tests the most extremely successful, but actual application is still subject to
To certain restriction, one of them main cause is exactly the technology needing to apply micro environment control aspect specialty.The most fully
Understand the physiological property of plantlet in container, the environment in container, the environment outside container, the physics of culture vessel or structural property
Between relation, on the contrary it will not be possible to be successfully applied to Photoautotrophic growth and grow system, use the minimum energy and raw material production high-quality
Plant.Photoautotrophic growth grows the failure that the complexity of control system can cause producing, and culture vessel is the field of plantlet growth
Institute, is one of most important influence factor in plant tissue culture, it is ensured that plantlet CO in culture vessel2Supply and concentration
Regulation and control are one of its key technologies, so must design and culture vessel that invention plant sugar-free is the most numerous and CO2Supplement supply system
System.
In view of this, nowadays in the urgent need to designing a kind of new culture systems, in order to overcome existing training method to exist
Drawbacks described above.
Summary of the invention
The technical problem to be solved is: provide a kind of plant sugar-free culture systems, it is ensured that plant is being cultivated
During, required illumination, CO2, temperature, humidity are sufficiently met, particularly can be according to plant growing situation and photosynthetic
Effect regulation air velocity, supplements CO2 gas and the effective supply of nutritional solution.Achieve answering of Sugar Free Plant Tissue Culture fast breeding technique
With and promote, the batch production automated production for seedling provides guarantee.
Additionally, the present invention also provides for a kind of plant cultivation container, it is ensured that plant in incubation, required illumination,
CO2, temperature, humidity are sufficiently met, and particularly can regulate air velocity according to plant growing situation and photosynthesis, mend
Fill the effective supply of CO2 gas and nutritional solution.Achieve application and the popularization of Sugar Free Plant Tissue Culture fast breeding technique, for the work of seedling
Factory's automated production provides guarantee.
For solving above-mentioned technical problem, the present invention adopts the following technical scheme that
A kind of plant sugar-free culture systems, described plant sugar-free culture systems includes: airtight culturing room, culturing rack, cultivation
Container, fan, CO2Steel cylinder, electromagnetic valve, steam line, CO2Densitometer, air-conditioning；
Size according to room in described airtight culturing room, places the culturing rack of several multilamellars, culturing rack is placed many
Individual culture vessel；CO2Steel cylinder is connected with electromagnetic valve by steam line, and electromagnetic valve is by steam line simultaneously and CO2Densitometer
It is connected with the air outlet of air-conditioning；
Described airtight culturing room is internal by air-conditioning temperature control, and the interior circulation of line space of going forward side by side gas, to ensure CO2Gas efficient
Utilize；
Described culture systems organically combines mandatory ventilation and natural ventilation, the CO of high concentration2Pass through air-conditioning
Air outlet mix with air after, be distributed in culturing room uniformly, then enter to cultivate by the air-vent on culture vessel and hold
Photosynthesis and growth promoter is carried out for plant in device；
Described culture systems is CO2Steel cylinder is connected with electromagnetic valve by donor pipeline, electromagnetic valve by pipeline simultaneously with
CO2Densitometer is connected with the air outlet of air-conditioning, makes CO2Concentration control and supply mode simple；
Equipped with fan on the culturing rack of described culture systems multilamellar, when container uses natural ventilation, the operation of fan promotees
Enter the exchange of air-flow inside and outside container, it is ensured that CO2The supply of gas；
The CO of high concentration in described steel cylinder2Gas is by electromagnetic valve control, by steam line at the air outlet of air-conditioning and sky
After gas mixing, delivered to each corner of airtight culturing room uniformly, then entered in container by the air-vent on culture vessel,
For cultivating used by plant；
The CO of airtight culturing room2Concentration is by electromagnetic valve and CO2Densitometer is connected to control, CO2Controlled concentration is: C3 plant
1000～2000ppm, C4 plant 2000～3000ppm, CO2The time of supply is Tong Bu with light application time；
Described culture vessel includes: culture vessel upper strata, culture vessel lower floor, nutritional solution unhurried current hole, nutritional solution output
Mouth, nutrient input entrance, compression pump, air-vent；
Described culture vessel divides upper and lower two-layer, and the dividing plate between the upper and lower two-layer of container leaves nutritional solution unhurried current hole, container
Lower floor leaves the delivery outlet of nutritional solution, and the input port of nutritional solution, input port and delivery outlet are left by nutritional solution pipe in container upper strata
Being connected with compression pump, top or the side of culture vessel have air-vent；
Described culture vessel is made up of transparent material, and culture vessel is cuboid or square or spherosome；
Leaving nutritional solution unhurried current hole on dividing plate between the upper and lower two-layer of described culture vessel, container lower floor leaves nutritional solution
Delivery outlet, container upper strata is left the input port of nutritional solution, input port and delivery outlet and is connected with compression pump by nutritional solution pipe, it is achieved
Circulating of nutritional solution；
Described culture vessel is divided into upper and lower two-layer, carries out solid culture, or carries out liquid culture and aerosol cultivation；Carry out
When liquid culture or aerosol are cultivated, there is external nutritional solution circulating line, the nutrient infusion of lower floor to upper strata, then pass through nutrition
Liquid unhurried current hole slowly flow back into lower floor and realizes nutritional solution recirculation；Only need to be by regulating the circulation time of nutritional solution, it is achieved various
Cultivate；If using gelatinous culture matrix, then need not recirculating nutrient solution；
Leaving nutritional solution unhurried current hole on dividing plate between the upper and lower two-layer of described culture vessel, unhurried current hole is one or more；Institute
Top or the side of stating culture vessel have air-vent, and air-vent is one or more, stick ventilated membrane and carry out natural ventilation；Or
Inserting trachea uses air pump and filter to carry out mandatory ventilation；Top or the side of described culture vessel have air-vent, thoroughly
Pore is one or more.
A kind of plant cultivation container, described plant cultivation container includes: described culture vessel includes: on culture vessel
Layer, culture vessel lower floor, nutritional solution unhurried current hole, nutrient input outlet, nutrient input entrance, compression pump；
Described culture vessel divides upper and lower two-layer, and the dividing plate between the upper and lower two-layer of container leaves nutritional solution unhurried current hole, container
Lower floor leaves the delivery outlet of nutritional solution, and the input port of nutritional solution, input port and delivery outlet are left by nutritional solution pipe in container upper strata
Being connected with compression pump, top or the side of culture vessel have air-vent.
As a preferred embodiment of the present invention, described culture vessel is made up of transparent material, and culture vessel is cuboid
Or square or spherosome.
As a preferred embodiment of the present invention, the dividing plate between the upper and lower two-layer of described culture vessel leaves nutritional solution and delays
Discharge orifice, container lower floor leaves the delivery outlet of nutritional solution, and container upper strata is left the input port of nutritional solution, input port and delivery outlet and passed through
Nutritional solution pipe is connected with compression pump, it is achieved circulating of nutritional solution.
As a preferred embodiment of the present invention, described culture vessel is divided into upper and lower two-layer, can carry out solid culture, liquid
Cultivate and aerosol is cultivated；When carrying out liquid culture or aerosol cultivation, there is external nutritional solution circulating line, the nutrient input of lower floor
Enter to upper strata, more slowly flow back into lower floor realize nutritional solution recirculation by nutritional solution unhurried current hole；Only need to be by regulation nutritional solution
Circulation time, it is achieved various cultivations；If using gelatinous culture matrix, then need not recirculating nutrient solution.
As a preferred embodiment of the present invention, the dividing plate between the upper and lower two-layer of described culture vessel leaves nutritional solution and delays
Discharge orifice, unhurried current hole is one or more；
The top of described culture vessel or side have air-vent, and air-vent is one or more, stick ventilated membrane and carry out certainly
So ventilation；Or insertion trachea uses air pump and filter to carry out mandatory ventilation；Top or the side of described culture vessel have
Air-vent, air-vent is one or more.
The present invention is to be realized, if carrying out solid culture can use agar by following cultural method, it is also possible to
The material of porous is as culture matrix, such as: Vermiculitum, perlite, peat soil etc., uses sugar-free culture-medium (not sugaring and organic
Thing, only adds a great number of elements, iron salt and trace element).Nutritional solution and substrate carry out high temperature or autoclaving, and culture vessel cleans
Can use after sterilizing with the hot-water soak of more than 70 degree after clean.If carrying out liquid culture or aerosol cultivation, cultivating and holding
The lower floor of device adds nutritional solution, and plant is cultivated on upper strata, realizes liquid training by the time of regulation nutritional solution supply and aerosol is trained；?
In incubation, the air inflow of logical regulation container meets plant growing and photosynthesis to CO2, the demand of air humidity.
The beneficial effects of the present invention is: the plant sugar-free culture systems that the present invention proposes, natural ventilation and pressure are changed
Two kinds of methods of gas combine, and during well solving plant culturing, supplement the CO2 in culture vessel and regulate asking of humidity
Topic, simplifies production routine；Solve tradition tissue culture present in growth and development of plants is bad, physiology and appearance is disorderly, abnormal
Shape, growth promoter delay or the problem such as death；The growth of plantlet can be remarkably promoted, improve seedling quality, shorten cultivation cycle
50%, significantly reduce production cost, beneficially the scale of plant fast propagation, batch production and automated production.
The present invention supplies CO2, gas circulates, temperature and humidity controls to combine, make CO2 distribution gentle
The motion of stream evenly, has effectively facilitated growth and the photosynthesis of plant, and plant strain growth is the most healthy and the strongest, well developed root system, transplants
Survival rate is high.
This invention simplifies technological process and the equipment of existing tissue culture technology, it is possible to use the cultivation that volume is big or little is held
Device, and make cultivation can take into account multiple training method, solid training, liquid training, substrate training, aerosol training etc. simultaneously, and operate steadily, can
Control property is strong, and management is simple, and operating cost is low.
The present invention the cycle-index by regulation nutritional solution, the air humidity controlled in culture vessel easily and effectively,
And create an optimal rhizospheric environment for plant growing, the water of flowing, the increase of dissolved oxygen, the culture medium of sugar-free promote plant
Self carries out photosynthesis, and these factors can promote root system of plant to be formed and growth, significantly improves the rooting rate of plantlet.
Accompanying drawing explanation
Fig. 1 is the composition schematic diagram of plant sugar-free culture systems of the present invention.
Fig. 2 is the structural representation of culture vessel of the present invention.
Detailed description of the invention
Describe the preferred embodiments of the present invention below in conjunction with the accompanying drawings in detail.
Embodiment one
Referring to Fig. 1, present invention is disclosed a kind of plant sugar-free culture systems, described plant sugar-free culture systems includes:
Airtight culturing room 1, culturing rack 2, culture vessel 3, fan 5, CO2Steel cylinder 6, electromagnetic valve 7, steam line 8, CO2Densitometer 9, air-conditioning
10。
The interior size according to room of described airtight culturing room 1, places the culturing rack 2 of several multilamellars, culturing rack 2 is placed
Multiple culture vessels 3；CO2Steel cylinder 6 is connected with electromagnetic valve 7 by donor pipeline 8, electromagnetic valve 7 by the while of steam line 8 with
CO2Densitometer 9 is connected with the air outlet of air-conditioning 10.Described airtight culturing room 1 is internal by air-conditioning 10 temperature control, line space of going forward side by side gas
Interior circulation, to ensure CO2The efficient utilization of gas.
Described culture systems organically combines mandatory ventilation and natural ventilation, the CO of high concentration2Pass through air-conditioning
Air outlet mix with air after, be distributed in culturing room uniformly, then enter to cultivate by the air-vent 4 on culture vessel and hold
Photosynthesis and growth promoter is carried out for plant in device；
Described culture systems is CO2Steel cylinder is connected with electromagnetic valve by donor pipeline, electromagnetic valve by pipeline simultaneously with
CO2Densitometer is connected with the air outlet of air-conditioning, makes CO2Concentration control and supply mode simple；
Equipped with fan on the culturing rack of described culture systems multilamellar, when container uses natural ventilation, the operation of fan promotees
Enter the exchange of air-flow inside and outside container, it is ensured that CO2The supply of gas；
The CO of high concentration in described steel cylinder2Gas, by electromagnetic valve control, passes through CO2Steam line air-conditioning air outlet with
After air mixing, delivered to each corner of airtight culturing room uniformly, then entered container by the air-vent on culture vessel
In, for cultivating used by plant；
The CO of airtight culturing room2Concentration is by electromagnetic valve and CO2Densitometer is connected to control, CO2Controlled concentration is: C3 plant
1000～2000ppm, C4 plant 2000～3000ppm, CO2The time of supply is Tong Bu with light application time.
Referring to Fig. 2, described culture vessel includes: culture vessel upper strata 31, culture vessel lower floor 32, nutritional solution unhurried current
Hole 33, nutrient input outlet 34, nutrient input entrance 35, compression pump 36, air-vent 37.
Described culture vessel divides upper and lower two-layer, and the dividing plate between the upper and lower two-layer of container leaves nutritional solution unhurried current hole, container
Lower floor leaves the delivery outlet of nutritional solution, and the input port of nutritional solution, input port and delivery outlet are left by nutritional solution pipe in container upper strata
Being connected with compression pump, top or the side of culture vessel have air-vent.
Described culture vessel is made up of transparent material, and culture vessel is cuboid or square or spherosome.Described cultivation
Leaving nutritional solution unhurried current hole on dividing plate between the upper and lower two-layer of container, container lower floor leaves the delivery outlet of nutritional solution, container upper strata
Leave the input port of nutritional solution, input port and delivery outlet to be connected with compression pump by nutritional solution pipe, it is achieved the recycle stream of nutritional solution
Dynamic.
Described culture vessel is divided into upper and lower two-layer, can carry out solid culture, liquid culture and aerosol and cultivate；Carry out liquid training
Support or aerosol cultivate time, have external nutritional solution circulating line, the nutrient infusion of lower floor to upper strata, then flow slowly by nutritional solution
Hole slowly flow back into lower floor and realizes nutritional solution recirculation；Only need to be by regulating the circulation time of nutritional solution, it is achieved various cultivations；As
Fruit is to use gelatinous culture matrix, then need not recirculating nutrient solution.
Leaving nutritional solution unhurried current hole on dividing plate between the upper and lower two-layer of described culture vessel, unhurried current hole is one or more；Institute
Top or the side of stating culture vessel have air-vent, and air-vent is one or more, stick ventilated membrane and carry out natural ventilation；Or
Inserting trachea uses air pump and filter to carry out mandatory ventilation；Top or the side of described culture vessel have air-vent, thoroughly
Pore is one or more.
Embodiment two
A kind of plant sugar-free culture systems, described plant sugar-free culture systems includes: airtight culturing room, culturing rack, cultivation
Container, air mixing machine structure, CO2Storage container, electromagnetic valve, steam line, CO2Densitometer, air-conditioning；
Size according to room in described airtight culturing room, places at least one culturing rack, culturing rack is placed at least one
Individual culture vessel；CO2Storage container is connected with electromagnetic valve by donor pipeline, and electromagnetic valve is by steam line simultaneously and CO2Dense
Degree meter is connected with the air outlet of air-conditioning；
Described airtight culturing room is internal by air-conditioning temperature control, and the interior circulation of line space of going forward side by side gas, to ensure CO2Gas efficient
Utilize；
Described culture systems organically combines mandatory ventilation and natural ventilation, the CO of high concentration2Pass through air-conditioning
Air outlet mix with air after, be distributed in culturing room uniformly, then enter to cultivate by the air-vent on culture vessel and hold
Photosynthesis and growth promoter is carried out for plant in device；
Described culture systems is CO2Storage container is connected with electromagnetic valve by donor pipeline, and electromagnetic valve is same by pipeline
Time and CO2Densitometer is connected with the air outlet of air-conditioning；
Equipped with air mixing machine structure on the culturing rack of described culture systems multilamellar, when container uses natural ventilation, air mixing machine structure
Run and promote the exchange of air-flow inside and outside container, it is ensured that CO2The supply of gas；
The CO of high concentration in described storage container2Gas, by electromagnetic valve control, passes through CO2Steam line is in the air-out of air-conditioning
After mouth mixes with air, delivered to each corner of airtight culturing room uniformly, then entered by the air-vent on culture vessel
In container, for cultivating used by plant.
Embodiment three
The present invention discloses a kind of air exchange system, and described air exchange system includes: airtight culturing room, culturing rack, culture vessel, thoroughly
Pore, fan, CO2 steel cylinder, electromagnetic valve, CO2 steam line, CO2 densitometer, air-conditioning.
Size according to room in described airtight culturing room, can place the culturing rack of multiple multilamellar, permissible on culturing rack
Place multiple culture vessel.CO2Steel cylinder is connected with electromagnetic valve by gas pipeline, and electromagnetic valve is by pipeline simultaneously and CO2Concentration
The air outlet of meter and air-conditioning is connected.
As a kind of preferred version of patent of the present invention, described airtight culturing room area is unrestricted, changeable,
But must be clean and sealing, culturing room be internal by air-conditioning temperature control, and the interior circulation of line space of going forward side by side gas, to ensure CO2Gas
Efficient utilization.
As a kind of preferred version of patent of the present invention, described air exchange system is organic to mandatory ventilation and natural ventilation knot
It is combined, after the CO2 of high concentration is mixed with air by the air outlet of air-conditioning, is distributed in culturing room uniformly, then passes through
Air-vent on culture vessel carries out photosynthesis and growth promoter for plant in entering culture vessel.
As a kind of preferred version of patent of the present invention, described air exchange system is CO2Steel cylinder passes through gas pipeline and electromagnetism
Valve is connected, and electromagnetic valve is by pipeline simultaneously and CO2The air outlet of densitometer and air-conditioning is connected, and makes the concentration of CO2 control and supply
Simple to mode.
As a kind of preferred version of patent of the present invention, equipped with fan on the culturing rack of described air exchange system multilamellar, work as appearance
When device uses natural ventilation, the operation of fan can effectively facilitate the exchange of air-flow inside and outside container, it is ensured that the supply of CO2 gas.
Its system operation mode is: in steel cylinder, the CO2 gas of high concentration is by electromagnetic valve control, is existed by CO2 steam line
After the air outlet of air-conditioning mixes with air, delivered to each corner of airtight culturing room uniformly, then by culture vessel
Air-vent enters in container, for cultivating used by plant.The CO2 concentration of culturing room is connected is controlled by electromagnetic valve and CO2 densitometer,
CO2 controlled concentration is: the time of C3 plant 1000-2000ppm, C4 plant 2000-3000ppm, CO2 supply is same with light application time
Step.
The present invention also discloses a kind of culture vessel, and described culture vessel includes: under culture vessel upper strata, culture vessel
Layer, nutritional solution unhurried current hole, nutrient input outlet, nutrient input entrance, compression pump, air-vent.
Described culture vessel divides upper and lower two-layer, and the dividing plate between the upper and lower two-layer of container leaves nutritional solution unhurried current hole, container
Lower floor leaves the delivery outlet of nutritional solution, and the input port of nutritional solution, input port and delivery outlet are left by nutritional solution pipe in container upper strata
Being connected with compression pump, top or the side of culture vessel have air-vent.
As a kind of preferred version of patent of the present invention, described culture vessel is made up of transparent material, its shape of culture vessel
Shape and size can arbitrarily set, and can be cuboid, square, spherosome etc.；
As a kind of preferred version of patent of the present invention, the dividing plate between the upper and lower two-layer of container leaves nutritional solution unhurried current
Hole, container lower floor leaves the delivery outlet of nutritional solution, and the input port of nutritional solution, input port and delivery outlet are left by battalion in container upper strata
Nutrient solution pipe is connected with compression pump, it is possible to achieve circulating of nutritional solution.
As a kind of preferred version of patent of the present invention, described culture vessel is divided into upper and lower two-layer, can carry out solid
Cultivate, it is also possible to carry out liquid culture and aerosol is cultivated；When carrying out liquid culture or aerosol cultivation, there is external nutritional solution circulation pipe
Road, the nutrient infusion of lower floor to upper strata, is more slowly flow back into lower floor realized nutritional solution recirculation by nutritional solution unhurried current hole.
Only just need to can realize various cultivation by regulating the circulation time of nutritional solution.If using gelatinous culture matrix such as
Agar, block that glue etc., then need not recirculating nutrient solution.
As a kind of preferred version of patent of the present invention, the dividing plate between the upper and lower two-layer of container leaves nutritional solution unhurried current
Hole, unhurried current hole can be one or more, quantity number can be according to the training method of plant: solid training, liquid training or aerosol training
Determine.
As a kind of preferred version of patent of the present invention, the top of culture vessel or side have air-vent, and air-vent can
To be one or more, ventilated membrane can be sticked and carry out natural ventilation；Also can be inserted into trachea uses air pump and filter to force
Property ventilation.
As a kind of preferred version of patent of the present invention, its shapes and sizes of culture vessel can arbitrarily set, and cultivates and holds
The top of device or side have air-vent, and air-vent can be one or more, and its most important factor is the ventilation time of container
Number, it is however generally that, the rate of ventilation of container is more suitable between 3-10, can be gradually increased with the prolongation of plant culturing time and change
Gas number of times.
In sum, the plant sugar-free culture systems that the present invention proposes, natural ventilation and two kinds of method phases of forced ventilation
In conjunction with, during well solving plant culturing, supplement the CO2 in culture vessel and the problem of regulation humidity, simplify life
Stages of labor sequence；Solve tradition tissue culture present in growth and development of plants is bad, physiology and appearance is disorderly, lopsided, growth promoter
Delay or the problem such as death；The growth of plantlet can be remarkably promoted, improve seedling quality, shorten cultivation cycle 50%, significantly drop
The scale of low production cost, beneficially plant fast propagation, batch production and automated production.
The present invention supplies CO2, gas circulates, temperature and humidity controls to combine, make CO2 distribution gentle
The motion of stream evenly, has effectively facilitated growth and the photosynthesis of plant, and plant strain growth is the most healthy and the strongest, well developed root system, transplants
Survival rate is high.
This invention simplifies technological process and the equipment of existing tissue culture technology, it is possible to use the cultivation that volume is big or little is held
Device, and make cultivation can take into account multiple training method, solid training, liquid training, substrate training, aerosol training etc. simultaneously, and operate steadily, can
Control property is strong, and management is simple, and operating cost is low.
The present invention the cycle-index by regulation nutritional solution, the air humidity controlled in culture vessel easily and effectively,
And create an optimal rhizospheric environment for plant growing, the water of flowing, the increase of dissolved oxygen, the culture medium of sugar-free promote plant
Self carries out photosynthesis, and these factors can promote root system of plant to be formed and growth, significantly improves the rooting rate of plantlet.
Here description of the invention and application is illustrative, is not wishing to limit the scope of the invention to above-described embodiment
In.The deformation of embodiments disclosed herein and change are possible, real for those skilled in the art
The various parts with equivalence of replacing executing example are known.It should be appreciated by the person skilled in the art that without departing from the present invention
Spirit or essential characteristics in the case of, the present invention can in other forms, structure, layout, ratio, and with other assembly,
Material and parts realize.In the case of without departing from scope and spirit of the present invention, embodiments disclosed herein can be entered
Other deformation of row and change.
Claims (6)
1. a plant sugar-free culture systems, it is characterised in that described plant sugar-free culture systems includes: airtight culturing room, training
Support frame, culture vessel, fan, CO2Steel cylinder, electromagnetic valve, steam line, CO2Densitometer, air-conditioning；
Size according to room in described airtight culturing room, places the culturing rack of several multilamellars, culturing rack is placed multiple training
Support container；CO2Steel cylinder is connected with electromagnetic valve by steam line, and electromagnetic valve is by steam line simultaneously and CO2Densitometer and sky
The air outlet adjusted is connected；
Described airtight culturing room is internal by air-conditioning temperature control, and the interior circulation of line space of going forward side by side gas, to ensure CO2The efficient utilization of gas；
Described culture systems organically combines mandatory ventilation and natural ventilation, the CO of high concentration2By the air-out of air-conditioning
After mouth mixes with air, it is distributed in culturing room uniformly, then enters the interior confession of culture vessel by the air-vent on culture vessel
Plant carries out photosynthesis and growth promoter；
Described culture systems is CO2Steel cylinder is connected with electromagnetic valve by donor pipeline, and electromagnetic valve is by pipeline simultaneously and CO2Dense
Degree meter is connected with the air outlet of air-conditioning, makes CO2Concentration control and supply mode simple；
Equipped with fan on the culturing rack of described culture systems multilamellar, when container uses natural ventilation, the operation of fan promotes to hold
The exchange of air-flow inside and outside device, it is ensured that CO2The supply of gas；
The CO of high concentration in described steel cylinder2Gas, by electromagnetic valve control, is mixed with air at the air outlet of air-conditioning by steam line
After, delivered to each corner of airtight culturing room uniformly, then entered in container by the air-vent on culture vessel, for cultivating
Used by plant；
The CO of airtight culturing room2Concentration is by electromagnetic valve and CO2Densitometer is connected to control, CO2Controlled concentration is: C3 plant 1000
～2000ppm, C4 plant 2000～3000ppm, CO2The time of supply is Tong Bu with light application time；
Described culture vessel includes: culture vessel upper strata, culture vessel lower floor, nutritional solution unhurried current hole, nutrient input outlet, battalion
Nutrient solution input port, compression pump, air-vent；
Described culture vessel divides upper and lower two-layer, and the dividing plate between the upper and lower two-layer of container leaves nutritional solution unhurried current hole, container lower floor
Leaving the delivery outlet of nutritional solution, the input port of nutritional solution, input port and delivery outlet are left by nutritional solution pipe and pressure in container upper strata
Power pump is connected, and top or the side of culture vessel have air-vent；
Described culture vessel is made up of transparent material, and culture vessel is cuboid or square or spherosome；
Leaving nutritional solution unhurried current hole on dividing plate between the upper and lower two-layer of described culture vessel, container lower floor leaves the output of nutritional solution
Mouthful, container upper strata is left the input port of nutritional solution, input port and delivery outlet and is connected with compression pump by nutritional solution pipe, it is achieved nutrition
Circulating of liquid；
Described culture vessel is divided into upper and lower two-layer, can carry out solid culture, liquid culture and aerosol and cultivate；Carry out liquid culture or
When aerosol is cultivated, there is external nutritional solution circulating line, the nutrient infusion of lower floor to upper strata, then delayed by nutritional solution unhurried current hole
Slug flow returns to lower floor and realizes nutritional solution recirculation；Only need to be by regulating the circulation time of nutritional solution, it is achieved various cultivations；If
Use gelatinous culture matrix, then need not recirculating nutrient solution；
Leaving nutritional solution unhurried current hole on dividing plate between the upper and lower two-layer of described culture vessel, unhurried current hole is one or more；Described training
Top or the side of supporting container have air-vent, and air-vent is one or more, sticks ventilated membrane and carries out natural ventilation；Or insert
Trachea uses air pump and filter to carry out mandatory ventilation；The top of described culture vessel or side have air-vent, air-vent
For one or more.
2. a plant cultivation container, it is characterised in that described plant cultivation container includes: described culture vessel includes: training
Support container upper strata, culture vessel lower floor, nutritional solution unhurried current hole, nutrient input outlet, nutrient input entrance, compression pump；
Described culture vessel divides upper and lower two-layer, and the dividing plate between the upper and lower two-layer of container leaves nutritional solution unhurried current hole, container lower floor
Leaving the delivery outlet of nutritional solution, the input port of nutritional solution, input port and delivery outlet are left by nutritional solution pipe and pressure in container upper strata
Power pump is connected, and top or the side of culture vessel have air-vent.
Plant cultivation container the most according to claim 2, it is characterised in that:
Described culture vessel is made up of transparent material, and culture vessel is cuboid or square or spherosome.
Plant cultivation container the most according to claim 2, it is characterised in that:
Leaving nutritional solution unhurried current hole on dividing plate between the upper and lower two-layer of described culture vessel, container lower floor leaves the output of nutritional solution
Mouthful, container upper strata is left the input port of nutritional solution, input port and delivery outlet and is connected with compression pump by nutritional solution pipe, it is achieved nutrition
Circulating of liquid.
Plant cultivation container the most according to claim 2, it is characterised in that:
Described culture vessel is divided into upper and lower two-layer, can carry out solid culture, liquid culture and aerosol and cultivate；Carry out liquid culture or
When aerosol is cultivated, there is external nutritional solution circulating line, the nutrient infusion of lower floor to upper strata, then delayed by nutritional solution unhurried current hole
Slug flow returns to lower floor and realizes nutritional solution recirculation；Only need to be by regulating the circulation time of nutritional solution, it is achieved various cultivations；If
Use gelatinous culture matrix, then need not recirculating nutrient solution.
Plant cultivation container the most according to claim 2, it is characterised in that:
Leaving nutritional solution unhurried current hole on dividing plate between the upper and lower two-layer of described culture vessel, unhurried current hole is one or more；
The top of described culture vessel or side have air-vent, and air-vent is one or more, stick ventilated membrane and carry out nature and change
Gas；Or insertion trachea uses air pump and filter to carry out mandatory ventilation；The top of described culture vessel or side have ventilative
Hole, air-vent is one or more.
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| CN201610669136.0A CN106172009B (en) | 2016-08-15 | 2016-08-15 | Plant sugar-free culture systems and plant cultivation container |
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| CN201610669136.0A CN106172009B (en) | 2016-08-15 | 2016-08-15 | Plant sugar-free culture systems and plant cultivation container |
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| CN106172009A true CN106172009A (en) | 2016-12-07 |
| CN106172009B CN106172009B (en) | 2018-08-21 |
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| CN106172009B (en) | 2018-08-21 |
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