CN113598051A - Anoectochilus roxburghii sugar-free tissue culture equipment and culture method - Google Patents
Anoectochilus roxburghii sugar-free tissue culture equipment and culture method Download PDFInfo
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- CN113598051A CN113598051A CN202110950193.7A CN202110950193A CN113598051A CN 113598051 A CN113598051 A CN 113598051A CN 202110950193 A CN202110950193 A CN 202110950193A CN 113598051 A CN113598051 A CN 113598051A
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- 241000934230 Anoectochilus roxburghii Species 0.000 title claims description 4
- 238000012136 culture method Methods 0.000 title abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 115
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 94
- 229920000742 Cotton Polymers 0.000 claims abstract description 47
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 47
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 45
- 239000002689 soil Substances 0.000 claims abstract description 28
- 238000003973 irrigation Methods 0.000 claims abstract description 22
- 230000002262 irrigation Effects 0.000 claims abstract description 22
- 238000010521 absorption reaction Methods 0.000 claims abstract description 18
- 238000001514 detection method Methods 0.000 claims abstract description 9
- 238000012360 testing method Methods 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 44
- 239000004745 nonwoven fabric Substances 0.000 claims description 22
- 241000719836 Anoectochilus formosanus Species 0.000 claims description 17
- 244000247747 Coptis groenlandica Species 0.000 claims description 6
- 235000002991 Coptis groenlandica Nutrition 0.000 claims description 6
- 240000002853 Nelumbo nucifera Species 0.000 claims description 6
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 6
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 230000008635 plant growth Effects 0.000 claims description 3
- 241000719837 Anoectochilus Species 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 4
- 230000000903 blocking effect Effects 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000009423 ventilation Methods 0.000 description 5
- 230000012010 growth Effects 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000001651 autotrophic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000011120 plywood Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention discloses a roxburgh anoectochilus terminal bud sugar-free tissue culture device and a culture method, and relates to the technical field of roxburgh anoectochilus terminal bud sugar-free tissue culture, in particular to a culture room placing frame and a culture box assembly, wherein a first conjunction block is fixed on the surface of a layer plate inside the culture room placing frame, the culture box assembly is fixed at the top of a second conjunction block, a culture tray is placed inside the box body, two sides of the inner wall of a U-shaped member are provided with occlusion teeth, the U-shaped member is connected with a cotton bar through the occlusion teeth, one end of a water conveying pipeline is connected with a water inlet pipe, the top of the box body is connected with a box cover, and a carbon dioxide detection device is arranged on the side face of a vertical plate of the culture room placing frame. According to the roxburgh anoectochilus terminal bud sugar-free tissue culture equipment and the culture method, the cotton rod blocks the water outlet hole, so that soil passes through the water outlet hole and enters the inside of the water conveying pipeline, the blocking phenomenon is avoided, and meanwhile, water is guided out from the inside of the water conveying pipeline based on the water absorption effect of the cotton rod and the water absorption rod to realize drip irrigation and provide water for test tube seedlings.
Description
Technical Field
The invention relates to the technical field of anoectochilus formosanus sugar-free tissue culture, in particular to anoectochilus formosanus sugar-free tissue culture equipment and a culture method.
Background
The anoectochilus roxburghii sugar-free tissue culture technology is also called photoautotrophic micropropagation technology, which is a novel plant micropropagation technology for changing the variety of carbon sources in plant tissue culture, replacing sugar with CO2 as the carbon source of a plant body, and promoting the photosynthesis of a plant by inputting CO2 gas as the carbon source and controlling environmental factors influencing the growth and development of test-tube plantlets to change the mixotrophic type of the test-tube plantlets into the autotrophic type so as to produce high-quality plantlets.
The existing gold thread lotus sugar-free tissue culture technology needs to use a culture box, test-tube seedlings are transplanted into the culture box, water is supplemented to the test-tube seedlings inside the culture box for water saving and better control of watering amount by adopting a drip irrigation mode, but because drip irrigation is carried out by burying a water pipe into soil, soil is easy to cause blockage when penetrating through a drip irrigation orifice and entering the water pipe.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a roxburgh anoectochilus terminal bud sugar-free tissue culture device and a culture method, which solve the problem that the existing roxburgh anoectochilus terminal bud sugar-free tissue culture technology proposed in the background art needs to use a culture box and transplant test-tube seedlings into the culture box, so that water is usually supplemented to the test-tube seedlings in the culture box in a drip irrigation mode for saving water and better controlling watering amount, but because a water pipe is buried in soil in the drip irrigation, the soil easily penetrates through a drip irrigation orifice and enters the water pipe to cause blockage.
In order to achieve the purpose, the invention is realized by the following technical scheme: a gold thread lotus sugar-free tissue culture device comprises a culture room rack and a culture box assembly, wherein a first conjunction block is fixed on the surface of a layer plate inside the culture room rack, the top of the first conjunction block is connected with a second conjunction block, the culture box assembly is fixed on the top of the second conjunction block, the culture box assembly comprises a box body, a culture tray, a water conveying pipeline, a water outlet hole, a U-shaped member, meshing teeth, a cotton stick and a water absorption rod, the culture tray is placed inside the box body, the water conveying pipeline is arranged inside the culture tray, the water outlet hole is formed in the surface of the water conveying pipeline, the U-shaped member is arranged inside the water outlet hole, the meshing teeth are arranged on two sides of the inner wall of the U-shaped member, the U-shaped member is connected with the cotton stick through the meshing teeth, the water absorption rod is arranged inside the cotton stick, one end of the water conveying pipeline is connected with a water inlet pipe, and the top of the box body is connected with a box cover, and the surface of the box cover is provided with a ventilation hole, and the side surface of the vertical plate of the culture room placing rack is provided with carbon dioxide detection equipment.
Optionally, the apopore is inside to be the back taper form, and the outer wall of U type profile and the apopore pore inner wall laminating.
Optionally, the U-shaped fastener is connected to the cotton swab through the water outlet holes, the engaging teeth, and the water outlet holes are equidistantly distributed on the surface of the water pipeline.
Optionally, the top of the first fitting block and the bottom of the second fitting block are both in a shape of a spur, and the top of the first fitting block fits with the bottom of the second fitting block.
Optionally, the culture box assembly further comprises a drain hole and a non-woven fabric layer, the drain hole is formed in the bottom of the culture tray, and the non-woven fabric layer is arranged inside the drain hole.
Optionally, the drainage holes are uniformly distributed at the bottom of the culture tray, and the outer opening structure of the non-woven fabric layer is matched with the inner opening structure of the drainage holes.
A method for culturing sugar-free tissue culture of anoectochilus formosanus comprises the following culturing steps:
s1, transplanting test-tube plantlets:
transplanting test-tube plantlets of anoectochilus formosanus into a culture tray, arranging the culture tray in the box body, connecting a water conveying pipeline with a water inlet pipe, then covering a box cover, and setting the temperature, the carbon dioxide content and the illumination intensity in a culture chamber;
s2, carbon dioxide monitoring:
the carbon dioxide detection equipment monitors the content of carbon dioxide in the culture room in real time, and controls the carbon dioxide supply device to supply carbon dioxide to the culture room when the content of carbon dioxide is insufficient until the indoor carbon dioxide reaches a preset value;
s3, drip irrigation:
when the test-tube plantlet needs to be subjected to drip irrigation, a water body is injected into the water conveying pipeline through the water inlet pipe, the water absorption rod absorbs liquid and transfers the liquid to the inside of the cotton rod, and the cotton rod absorbs the liquid more along with the increase of the liquid absorption rod, so that the liquid is separated out and seeped into soil, and the root system of the test-tube plantlet can be absorbed conveniently;
s4, discharging water liquid:
redundant liquid seeps to the bottom of the soil and flows to the inside of the box body through the drainage hole after drip irrigation, solid-liquid analysis is realized through the non-woven fabric layer, the liquid seeps through the non-woven fabric layer and continues to slide downwards, and the soil is reserved inside the culture tray.
Optionally, in the step S1, a plug-in movable connection manner is adopted between the water conveying pipeline and the water inlet pipe.
Optionally, in the step S3, the water body may be replaced with a nutrient solution required for plant growth according to actual conditions.
The invention provides a roxburgh anoectochilus terminal bud sugar-free tissue culture device and a culture method, which have the following beneficial effects:
1. according to the roxburgh anoectochilus terminal bud sugar-free tissue culture equipment and the culture method, the cotton rod blocks the water outlet hole, so that soil passes through the water outlet hole and enters the inside of the water conveying pipeline, the blocking phenomenon is avoided, and meanwhile, water is guided out from the inside of the water conveying pipeline based on the water absorption effect of the cotton rod and the water absorption rod to realize drip irrigation and provide water for test tube seedlings.
2. This gold thread lotus sugar-free group banks up equipment and cultivation method with earth, inside the apopore was inserted to the cotton stick after being wrapped up by U type odd piece, thereby U type odd piece both ends atress was close to the centre gripping cotton stick, and the interlock tooth card is gone into the cotton stick inside in order to improve the centre gripping firmness simultaneously, is favorable to preventing that the cotton stick is pushed out the apopore inside under the water flow effect, and the later stage is held between the fingers U type odd piece both ends simultaneously and is close to each other and lift and can take out the cotton stick, conveniently replaces it.
3. According to the roxburgh anoectochilus terminal bud sugar-free tissue culture equipment and the culture method, the top of the first conjunction block is in conjunction with the bottom of the second conjunction block, so that the box body is stably placed on the surface of the laminate of the culture room placing frame, the box body is prevented from sliding, and the first conjunction block and the second conjunction block can be separated only by lifting the box body, so that the box body is convenient to take down.
4. According to the gold thread lotus sugar-free tissue culture equipment and the culture method, after drip irrigation, redundant liquid seeps downwards to the bottom of soil and flows into the box body through the drainage holes, solid-liquid analysis is achieved through the non-woven fabric layer, the liquid seeps through the non-woven fabric layer and continuously slides downwards, soil is reserved inside the culture tray, and the soil is prevented from running off along with liquid discharge.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the case according to the present invention;
FIG. 3 is a schematic view of the internal structure of the water outlet hole of the present invention;
FIG. 4 is an enlarged view of the structure at A in FIG. 2 according to the present invention;
FIG. 5 is an enlarged view of the structure at B in FIG. 2 according to the present invention.
In the figure: 1. placing a culture room rack; 2. a first engagement block; 3. a second engagement block; 4. a culture box assembly; 401. a box body; 402. a culture tray; 403. a water delivery pipeline; 404. a water outlet hole; 405. a U-shaped profile; 406. engaging teeth; 407. a cotton swab; 408. a water-absorbing rod; 409. a drain hole; 410. a non-woven fabric layer; 5. a water inlet pipe; 6. a box cover; 7. a ventilation hole; 8. carbon dioxide detection equipment.
Detailed Description
Referring to fig. 1 to 5, the present invention provides a technical solution: a sugarless gold thread lotus tissue culture device comprises a culture room rack 1 and a culture box assembly 4, wherein a first conjunction block 2 is fixed on the surface of a layer plate in the culture room rack 1, the top of the first conjunction block 2 is connected with a second conjunction block 3, the culture box assembly 4 is fixed on the top of the second conjunction block 3, the culture box assembly 4 comprises a box body 401, a culture tray 402, a water conveying pipeline 403, a water outlet hole 404, a U-shaped component 405, a meshing tooth 406, a cotton stick 407 and a water absorption rod 408, the culture tray 402 is placed in the box body 401, the water conveying pipeline 403 is arranged in the culture tray 402, the surface of the water conveying pipeline is provided with a water outlet hole 404, the U-shaped component 405 is arranged in the water outlet hole 404, the meshing teeth 406 are arranged on two sides of the inner wall of the U-shaped component 405, the U-shaped component 405 is connected with the cotton stick 407 through the meshing teeth 406, and the water absorption rod 408 is arranged in the cotton stick 407, one end of the water conveying pipeline 403 is connected with a water inlet pipe 5, the top of the box body 401 is connected with a box cover 6, the surface of the box cover 6 is provided with a ventilation hole 7, and the side surface of a vertical plate of the culture room placing frame 1 is provided with a carbon dioxide detection device 8;
the specific operation is as follows, test-tube plantlets of anoectochilus formosanus are transplanted into a culture tray 402, the culture tray 402 is arranged in a box body 401, a second conjunction block 3 at the bottom of the box body 401 is made to be consistent with a first conjunction block 2, the box body 401 is stably arranged on the surface of a laminate of a culture room placing frame 1 at the moment, a water conveying pipeline 403 is connected with a water inlet pipe 5, then a box cover 6 is covered, the temperature, the carbon dioxide content and the illumination intensity in the culture room are set, the carbon dioxide content in the culture room is monitored in real time through a carbon dioxide detection device 8, a carbon dioxide supply device is controlled to supply carbon dioxide to the culture room when the carbon dioxide content is insufficient until the indoor carbon dioxide reaches a preset value required by growth of the test-tube plantlets of the anoectochilus formosanus, and the ventilation holes 7 are arranged on the surface of the box cover 6 to facilitate indoor air circulation, so that the test-tube plantlets can absorb the carbon dioxide in the air for culture, when then need driping irrigation the test tube seedling, by inlet tube 5 to the inside water injection body of conduit 403, water absorption rod 408 absorbs liquid and transmits to inside cotton swab 407, cotton swab 407 increases along with its absorption liquid, lead to liquid to separate out infiltration to soil in, thereby so that test tube seedling's root system absorbs, and cotton swab 407 is inserted apopore 404 after being wrapped up by U type other 405 inside, thereby U type other 405 both ends atress is close to centre gripping cotton swab 407, interlock tooth 406 card is inside in order to improve the centre gripping firmness simultaneously, be favorable to preventing that cotton swab 407 from being pushed out apopore 404 under the water flow effect inside, hold between the two ends of U type other 405 each other and lift and can take out cotton swab 407 in later stage simultaneously, the convenience is replaced it.
As shown in fig. 3, the inside of the water outlet 404 is inverted cone-shaped, and the outer wall of the U-shaped fastener 405 is attached to the inner wall of the water outlet 404;
when the cotton swab 407 is wrapped by the U-shaped fastener 405 and inserted into the water outlet 404, the two ends of the top of the U-shaped fastener 405 are closed based on the internal shape of the water outlet 404, so that the cotton swab 407 is clamped and prevented from falling off.
As shown in fig. 2 and 3, the U-shaped profile 405 is engaged with the cotton swab 407 through the outlet holes 404 and the engaging teeth 406, and the outlet holes 404 are equidistantly distributed on the surface of the water pipe 403;
based on the soft characteristic of cotton swab 407, interlock tooth 406 can block into inside cotton swab 407 when U type profile 405 folds, is favorable to improving the clamping force to cotton swab 407, prevents that it from taking place to drop by the influence that the water body flows, and apopore 404 is equidistance form distribution and can make cotton swab 407 evenly distributed in soil on conduit 403 surface to make test tube plantlet evenly absorb moisture.
As shown in fig. 1-2, the top of the first fitting block 2 and the bottom of the second fitting block 3 are both in a stabbing shape, and the top of the first fitting block 2 fits with the bottom of the second fitting block 3;
when box body 401 settled in the plywood surface of cultivateing room rack 1, the top of first agreeing with piece 2 agrees with the bottom of piece 3 with the second and agrees with, should set up and make box body 401 equidistance neatly arrange, improve pleasing to the eye degree, also difficult emergence separation simultaneously avoids box body 401 to have the accident to take place to slide the cultivation that influences test tube seedling.
As shown in fig. 5, the culture box assembly 4 further comprises a drainage hole 409 and a non-woven fabric layer 410, the bottom of the culture tray 402 is provided with the drainage hole 409, and the non-woven fabric layer 410 is arranged inside the drainage hole 409;
specifically, after the drip irrigation, the excess liquid in the soil seeps down to the bottom of the soil and flows into the box 401 through the drainage hole 409, the solid-liquid analysis is performed by the nonwoven fabric layer 410, the liquid seeps down the nonwoven fabric layer 410, and the soil is retained in the culture tray 402.
As shown in fig. 2 and 5, the drainage holes 409 are uniformly distributed at the bottom of the culture tray 402, and the outer opening structure of the non-woven fabric layer 410 is matched with the inner opening structure of the drainage holes 409;
the drain holes 409 are uniformly distributed at the bottom of the culture tray 402, so that redundant moisture at each part in the soil can be drained, and the phenomenon that the growth of test-tube plantlets is influenced by the deposition of moisture in the culture tray 402 is prevented.
A method for culturing sugar-free tissue culture of anoectochilus formosanus comprises the following culturing steps:
s1, transplanting test-tube plantlets:
transplanting test-tube plantlets of anoectochilus formosanus into a culture tray 402, arranging the culture tray 402 in a box body 401, connecting a water conveying pipeline 403 with a water inlet pipe 5, then covering a box cover 6, and setting the temperature, the carbon dioxide content and the illumination intensity in a culture chamber;
s2, carbon dioxide monitoring:
the carbon dioxide detection device 8 monitors the content of carbon dioxide in the culture room in real time, and controls the carbon dioxide supply device to supply carbon dioxide to the culture room when the content of carbon dioxide is insufficient until the indoor carbon dioxide reaches a preset value;
s3, drip irrigation:
when the test-tube plantlet needs to be subjected to drip irrigation, a water body is injected into the water conveying pipeline 403 through the water inlet pipe 5, the water absorption rod 408 absorbs liquid and transfers the liquid to the inside of the cotton rod 407, and the cotton rod 407 absorbs the liquid more along with the increase of the liquid absorption, so that the liquid is separated out and seeped into soil, and the root system of the test-tube plantlet is absorbed conveniently;
s4, discharging water liquid:
after drip irrigation, the excessive liquid seeps to the bottom of the soil and flows to the inside of the box body 401 through the drainage hole 409, solid-liquid analysis is realized through the non-woven fabric layer 410, the liquid seeps the non-woven fabric layer 410 to slide down continuously, and the soil is kept inside the culture tray 402.
In step S1, the water pipe 403 and the water inlet pipe 5 are connected by a plug-in movable connection.
In the step S3, the water body can be replaced by nutrient solution required by plant growth according to actual conditions.
In summary, the roxburgh anoectochilus terminal bud sugar-free tissue culture equipment and the method for culturing the same are used, firstly, test-tube seedlings of the roxburgh anoectochilus terminal bud are transplanted into the culture tray 402, the culture tray 402 is arranged in the box body 401, then the second conjunction block 3 at the bottom of the box body 401 is made to be in conjunction with the first conjunction block 2, at the moment, the box body 401 is stably arranged on the surface of a laminate of the placing frame 1 of the culture room, and then the water conveying pipeline 403 is connected with the water inlet pipe 5;
then, the box cover 6 is covered, the temperature, the carbon dioxide content and the illumination intensity in the culture chamber are set, the carbon dioxide content in the culture chamber is monitored in real time through the carbon dioxide detection equipment 8, the carbon dioxide is supplied to the culture chamber by the carbon dioxide supply device when the carbon dioxide content is insufficient, and the indoor carbon dioxide reaches a preset value required by growth of the anoectochilus formosanus test-tube plantlets, and the ventilation holes 7 are formed in the surface of the box cover 6, so that the indoor air circulation is facilitated, and the test-tube plantlets can absorb the carbon dioxide in the air for cultivation;
then when the test-tube plantlet needs to be subjected to drip irrigation, a water body is injected into the water conveying pipeline 403 through the water inlet pipe 5, the water absorption rod 408 absorbs liquid and transmits the liquid to the inside of the cotton rod 407, the cotton rod 407 increases along with the absorbed liquid, so that the liquid is separated out and seeps into soil, the root system of the test-tube plantlet is absorbed, the cotton rod 407 is wrapped by the U-shaped part 405 and then inserted into the water outlet hole 404, the two ends of the U-shaped part 405 are stressed to be close to each other so as to clamp the cotton rod 407, meanwhile, the meshing teeth 406 are clamped into the cotton rod 407 to improve clamping firmness, the cotton rod 407 is prevented from being pushed out of the water outlet hole 404 under the action of water flow, meanwhile, the two ends of the U-shaped part 405 are clamped to be close to each other and lifted up, and the cotton rod 407 can be taken out, and the replacement of the cotton rod is facilitated;
finally, after drip irrigation, redundant liquid in the soil seeps to the bottom of the soil and flows to the inside of the box body 401 through the drain hole 409, solid-liquid analysis is realized through the non-woven fabric layer 410, the liquid seeps through the non-woven fabric layer 410 and slides downwards continuously, and the soil is kept inside the culture tray 402.
Claims (9)
1. The utility model provides a gold thread lotus sugar-free group banks up equipment with earth, includes cultivates room rack (1) and cultivates box subassembly (4), its characterized in that: a first conjunction block (2) is fixed on the surface of a layer plate in the culture chamber placing frame (1), a second conjunction block (3) is connected to the top of the first conjunction block (2), a culture box assembly (4) is fixed on the top of the second conjunction block (3), the culture box assembly (4) comprises a box body (401), a culture tray (402), a water conveying pipeline (403), a water outlet hole (404), a U-shaped part (405), a meshing tooth (406), a cotton rod (407) and a water absorbing rod (408), the culture tray (402) is placed in the box body (401), the water conveying pipeline (403) is arranged in the culture tray (402), the water outlet hole (404) is formed in the surface of the water conveying pipeline (403), the U-shaped part (405) is arranged in the water outlet hole (404), the meshing teeth (406) are arranged on two sides of the inner wall of the U-shaped part (405), u type profile (405) is connected with cotton-padded stick (407) through interlock tooth (406), and the inside of cotton-padded stick (407) is provided with water absorption stick (408), the one end of hydraulic pipeline (403) is connected with inlet tube (5), the top of box body (401) is connected with lid (6), and the surface of lid (6) has seted up draught hole (7), the riser side of cultivateing room rack (1) is provided with carbon dioxide check out test set (8).
2. The anoectochilus formosanus sugar-free tissue culture equipment according to claim 1, which is characterized in that: apopore (404) is inside to be the back taper form, and the outer wall and apopore (404) inner wall of U type profile (405) laminate mutually.
3. The anoectochilus formosanus sugar-free tissue culture equipment according to claim 1, which is characterized in that: the U-shaped fastener (405) is connected with the cotton swab (407) in a clamping manner through the water outlet holes (404) and the meshing teeth (406), and the water outlet holes (404) are equidistantly distributed on the surface of the water conveying pipeline (403).
4. The anoectochilus formosanus sugar-free tissue culture equipment according to claim 1, which is characterized in that: the top of the first conjunction block (2) and the bottom of the second conjunction block (3) are both in a protruding thorn shape, and the top of the first conjunction block (2) is in conjunction with the bottom of the second conjunction block (3).
5. The anoectochilus formosanus sugar-free tissue culture equipment according to claim 1, which is characterized in that: the culture box assembly (4) further comprises a drain hole (409) and a non-woven fabric layer (410), the drain hole (409) is formed in the bottom of the culture tray (402), and the non-woven fabric layer (410) is arranged inside the drain hole (409).
6. The anoectochilus formosanus sugar-free tissue culture equipment according to claim 5, which is characterized in that: the drain holes (409) are uniformly distributed at the bottom of the culture tray (402), and the outer opening structure of the non-woven fabric layer (410) is matched with the inner opening structure of the drain holes (409).
7. The method for sugar-free tissue culture of Anoectochilus roxburghii according to any one of claims 1-6, which is characterized in that: comprises the following culture steps:
s1, transplanting test-tube plantlets:
transplanting test-tube plantlets of anoectochilus formosanus into a culture tray (402), arranging the culture tray (402) in a box body (401), connecting a water conveying pipeline (403) with a water inlet pipe (5), then covering a box cover (6), and setting the temperature, the carbon dioxide content and the illumination intensity in a culture room;
s2, carbon dioxide monitoring:
the carbon dioxide detection equipment (8) monitors the content of carbon dioxide in the culture room in real time, and controls the carbon dioxide supply device to supply carbon dioxide to the culture room when the content of carbon dioxide is insufficient until the indoor carbon dioxide reaches a preset value;
s3, drip irrigation:
when the test-tube plantlet needs to be subjected to drip irrigation, a water body is injected into the water conveying pipeline (403) through the water inlet pipe (5), the water absorption rod (408) absorbs liquid and transfers the liquid to the inside of the cotton rod (407), and the cotton rod (407) absorbs the liquid more along with the increase of the liquid absorption, so that the liquid is separated out and seeped into soil, and the root system of the test-tube plantlet is absorbed conveniently;
s4, discharging water liquid:
after drip irrigation, redundant liquid seeps to the bottom of the soil and flows to the interior of the box body (401) through the drain hole (409), solid-liquid analysis is realized through the non-woven fabric layer (410), the liquid seeps through the non-woven fabric layer (410) and slides downwards continuously, and the soil is kept in the culture tray (402).
8. The method for sugar-free tissue culture of anoectochilus formosanus according to claim 7, which comprises the following steps: in the step S1, a plug-in movable connection mode is adopted between the water conveying pipeline (403) and the water inlet pipe (5).
9. The method for sugar-free tissue culture of anoectochilus formosanus according to claim 7, which comprises the following steps: in the step S3, the water body may be replaced with a nutrient solution required for plant growth according to actual conditions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110950193.7A CN113598051A (en) | 2021-08-18 | 2021-08-18 | Anoectochilus roxburghii sugar-free tissue culture equipment and culture method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110950193.7A CN113598051A (en) | 2021-08-18 | 2021-08-18 | Anoectochilus roxburghii sugar-free tissue culture equipment and culture method |
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| Publication Number | Publication Date |
|---|---|
| CN113598051A true CN113598051A (en) | 2021-11-05 |
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| CN202110950193.7A Pending CN113598051A (en) | 2021-08-18 | 2021-08-18 | Anoectochilus roxburghii sugar-free tissue culture equipment and culture method |
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Application publication date: 20211105 |